scholarly journals Relationship between the Characteristics of Bread Wheat Grains, Storage Time and Germination

Plants ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 35
Author(s):  
Dmitry A. Afonnikov ◽  
Evgenii G. Komyshev ◽  
Vadim M. Efimov ◽  
Mikhail A. Genaev ◽  
Vasily S. Koval ◽  
...  

Seed storage is important to farmers, breeders and for germplasm preservation. During storage, seeds accumulate damage at the structural and metabolic level, which disrupt their function and reduce resistance to adverse external conditions. In this regard, issues related to seed aging prove to be relevant for maintaining the viability of genetic collections. We analyzed morphological characteristics of grains and their coat color for 44 recombinant inbred lines (RILs) of bread wheat grown in four different seasons, 2003, 2004, 2009 and 2014. Our investigations were performed in 2020. For 19 RILs from the same seasons germination was evaluated. Our results demonstrate that genotype significantly affects the variability of all seed traits, and the year of harvesting affects about 80% of them (including all the traits of shape and size). To identify the trend between changes in grain characteristics and harvesting year, we estimated correlation coefficients between them. No significant trend was detected for the grain shape/size traits, while 90% of the color traits demonstrated such a trend. The most significant negative correlations were found between the harvesting year and the traits of grain redness: the greater the storage time, the more intensive is red color component for the grains. At the same time, it was shown that grains of longer storage time (earlier harvesting year) have lighter coat. Analysis of linear correlations between germination of wheat seeds of different genotypes and harvesting years and their seed traits revealed a negative linear relationship between the red component of coat color and germination: the redder the grains, the lower their germination rate. The results obtained demonstrate manifestations of metabolic changes in the coat of grains associated with storage time and their relationship with a decrease of seed viability.

2021 ◽  
Vol 42 ◽  
pp. e69341
Author(s):  
Miguel Ángel González Pérez ◽  
Nereida Cabrera-García ◽  
Isabel Cayon-Fernández

Conservation seed banks are essential for ex-situ conservation of genetic biodiversity. These institutions are especially relevant for threatened species and play a vital role in their conservation by preserving genetic material. However, samples deposited in the seed banks must germinate when necessary to use them (i.e., recovery plans, etc.). This study uses four accessions of the endemic endangered species from Gran Canaria Island (Canary Islands), Isoplexis isabelliana (Webb & Berthel.) Masf. (Scrophulariaceae). Germination tests were carried out to measure seed viability through time and the possible impact of seed storage on their viability. These accessions have been kept in the seed bank for four months to thirty years under different storage conditions. Germination results differed for seeds after 45 days of exposition using 16 hours light and 8 hours darkness at 17 °C. Accessions kept in the seed bank, independently of storage, showed a high germination percentage (89%). Whereas the accessions with rough storage conditions showed a 0% germination rate. The results highlighted the good state of conservation of the material deposited in the Seed Bank of the Botanical Garden "Viera y Clavijo" and the reliability of the temperature and humidity conditions in which the seeds of I. isabelliana have been stored. We consider these results as momentous since several natural populations of I. isabelliana has been affected by the last forest fire on the island.


Author(s):  
Mustafa Yıldırım

In semi-arid regions, unfavorable weather conditions are the most important limiting factors for seed germination. The imbibed or germinating seeds in soil can be dried by hot and dry weather. Thus, seeds can largely lose their germination abilities until the next water uptake (called as ‘alatav’ in Turkish). Before drying, the time of the first water uptake by a seed may be more effective on seed viability at the second water uptake for germination. Therefore, this experiment was conducted to identify the first water uptake rates (WU) of seeds of three bread wheat genotypes (Bezotaja, Kirac and 13-BVD-4) per hour during 50 hours and their germination rates (GR) at the second water uptake after drying the seeds for 3-week in laboratory conditions. The results presented here indicated that the WU and GR were significantly affected by genotypes, imbibition time (T) and their interaction (1% probability level). There was also a significant negative correlation between the WU and GR (r** = –0.9295) at all times studied. Furthermore, the germination ability of Kirac lasted longest (for 38th hour) when the WU reached to 160.24%, and it was followed by Bezostaya and 13-BVD-4 genotypes (63.34% at 37th hour and 152.58% 34th hour, respectively).


Author(s):  
Hayati Akman

This study targeted to elucidate the effect of seed aging on germination and emergence rates with and shoot characteristics in wheat cultivars. For this purpose, different bread wheat cultivars stored for 7 years and non-stored were compared for coleoptile length, root mass, shoot mass, root length as well as germination and seedling emergence rates. Here, the evidence suggested that seed storage over a prolonged period affected root and Shoot growth, coleoptile length, seed germination, and seedling emergence rates adversely. By linking germination and emergence rates, the data presented here indicated that a reduction in emergence rate in long-term storage was higher than that in the germination rate. It was also found that there were significant variations among the wheat cultivars about investigated traits during long-term storage. However, the emergence rates of Kate A1 and Flamura 85 were not affected substantially by long-term storage. The study suggested future studies to focus on clarification of the process controlling natural seed aging as such knowledge allows clue the eventual consequences of long-term storage.


2016 ◽  
Vol 4 (2) ◽  
pp. 69
Author(s):  
Fitri Lestari Manurung ◽  
Melya Riniarti ◽  
Duryat .

Jengkol (Pithecellobium lobatum) is one of MPTs that is included of Fabaceae Family.  Jengkol seed is counted as recalcitrant, which is could not defend it is viability when it is stored at low temperature and humidity.  The study aims were to determine the effect of storage time and storage media to storability of jengkol seed, storage media effective to  maintain the jengkol seed viability, and the interaction between the storage media and the storage time to jengkol seed.  The reseach design of factorial (3 x 4) in a completely randomized was employed as a research method.  The first factor was the storage time (T) which was consisted of T0 (without storage), T1 (2 weeks of storage), T2 (4 weeks of storage), and T3 (6 weeks of storage). Second factor was storage media (M) which was consisted of M0 (without storage media), M1 (sawdust storage media), and M2 (rice husk storage media).  For each combination treatment was repeated 3 times.  Each unit of experiments consisted of 40 seeds.  The observed variables were germinated seeds in storage, the number of germinated seeds, avarage day to germination and germination power.  Bartlett test was used to figure out the homogenity of datas.  Varians analysis was at least one used to see if there was at least one significant treatment. The smalles significant differences test was used as further test.  The entire data were tested on 5% significant level. The results showed that storage of jengkol seed using rice husk for storage media with storage time of 6 weeks gave the best effect for maintaining the shelf life of the seeds.  The most effective media to maintain the viability of jengkol seeds is rice husk which can defend the jengkol seeds viability up to 6 weeks of storage.  Keywords : jengkol, germination, storage media, storage time


HortScience ◽  
1991 ◽  
Vol 26 (8) ◽  
pp. 1054-1057 ◽  
Author(s):  
W.J. Carpenter ◽  
G.J. Wilfret ◽  
J.A. Cornell

Gladiolus (G. grandiflorus) seed germination was light-independent, but temperature influenced the germination rate. Constant 20C promoted higher total germination (97%), fewer days (4.3) to 50% of final germination, and shorter span of days (4.8) between 10% and 90% germination than other constant temperatures, although similar results were achieved by alternating 12-h cycles of 20 to 25C. Total germination was unchanged after seed treatment for 7 days at 10 to -20C, but longer germination periods were required after treatments below -10C. Reducing seed moisture contents from 11.8% to 4.2% caused no reduction in total germination, but moisture contents below 6.6% delayed achieving 50% of final germination and extended the periods from 10% to 90% of germination. Temperature and relative humidity (RH) during storage were important in retaining seed viability, with RH having a larger effect. Smallest declines in total germination during 12 months of storage occurred at 11% and 33% RH at 15C. The statistical analysis estimated the optimum seed storage at 14C and 26% RH.


2020 ◽  
Vol 8 (2) ◽  
pp. 327
Author(s):  
Iska Hartina Anggraini ◽  
Muhammad Kamal ◽  
Eko Pramono ◽  
Kukuh Setiawan

Sorghum is a type of carbohydrate-producing serelia plant. Sorghum has a great potential to be developed in Indonesia.  Seed storing time is the time period of the seed storage before the seed is planted again, while the storage of the seed is carried out with the aim of maintaining seed viability in the save period for as long as possible and can be used for the next planting period.  The purpose of this study was to determine the effect of storage time on seed vigor and sprouts of Kawali and P/F-10-90A genotypes. This research was conducted at the Laboratory of Seed and Plant Breeding, Faculty of Agriculture, University of Lampung, Bandar Lampung which runs from February 2017 until February 2018. This study uses split plot design with 3 group replications. The main plot is the storage time (LS) that was consisted of 0, 4, 8 and 12 months storage time period. Subplot is genotype (G), which consists of Kawali (G1 ) and P / F-10-90A (G2). The results showed that seed vigor and sprouts decreased significantly at 4 and 8 months storage time with the percentage of dead seeds 16.7% and 40.7%, while the percentage of normal strong sprouts at 4 months amounted to 74% for genotype P/ F-10-90A  and 8 months 54% for genotype Kawali.  P/F-10 -90A genotypes have higher seed vigor and sprouts vigor compared to Kawali genotypes as indicated by dead seed variables, strong normal sprouts, normal sprout length canopy, long root primary roots, normal sprouts, dry weight normal sprouts, and normally strong sprouts. The effect of interaction  between storage time and genotype was shown by seed vigor of genotype P/F-10-90-A seedlings which were proven to be superior for 4, 8 and 12-month storage time period.  Excellence of genotype P/F-10-90-A make it can be stored and was allowed to be used as good seed on next season farming.


1991 ◽  
Vol 15 (4) ◽  
pp. 216-221 ◽  
Author(s):  
F. T. Bonner

Abstract The effect of cone storage on seed viability during 5 years of storage at 35°F was tested with seeds from studies with loblolly (Pinus taeda L.), slash (P. elliottii Engelm.), longleaf (P. palustris Mill.), and eastern white (P. strobus L.) pines. Results showed that: (1) with the exception of slash pine, seeds from cones stored for the longer periods retained quality during storage better than seeds from cones that were stored only short periods; (2) all species exhibited slight, but statistically significant, decreases in seed quality after 3 years of storage, and with the exception of loblolly, more substantial decreases in germination rate at 5 years; (3) with the exception of eastern white pine, declining rates of germination indicated losses of seed quality at least one year before total percent germination decreased, and (4) slash pine seeds from cones in the center of 20-bushel crates performed better in storage than those from cones at the top of the crates or from cones stored in bags, but storage container made no difference for loblolly. South. J. Appl. For. 15(4):216-221


2020 ◽  
Vol 21 (10) ◽  
Author(s):  
ISKANDAR Z. SIREGAR ◽  
KARIMA FAUZIAH MUHARAM ◽  
Y. ARIS PURWANTO ◽  
DEDE J. SUDRAJAT

Abstract. Siregar IZ, Muharam KF, Purwanto YA, Sudrajat DJ. 2020. Seed germination characteristics in different storage time of Gmelina arborea treated with ultrafine bubbles priming. Biodiversitas 21: 4558-4564.  Gmelina (Gmelina arborea) seed collected from tropical Indonesian forest experience seed deterioration during storage which is relatively faster than the gmelina seeds originating from temperate regions, such as India and Myanmar. Various treatments have been made to improve the seed viability and vigor after storage through various invigoration techniques. However, the utilization of promising novel technology such as ultrafine bubbles (UFB) has not been evaluated yet. The objective of this study was to determine the effect of seed priming using UFB, polyethylene glycol (PEG), and gibberellic acid (GA3) on the viability and vigor of gmelina seeds that have been stored for one and two years. This study was conducted by employing Completely Randomized Design (CRD) using three replicates. The study showed priming treatment had significant effect and formed average germination capacity of 80% for the seed stored for 1 year.  On the other hand, the priming treatment using PEG-0.8 MPa was the best treatment for germination capacity of 74.67% on 2 years stored seeds. UFB had significant effect on germination capacity, germination rate, growth uniformity, and germination value. This result was expected due to reactive oxygen species (ROS) produced by micro-nano bubbles that could increase physiological activity of the seed cells.


2018 ◽  
Vol 3 (2) ◽  
pp. 89
Author(s):  
Yulistiati Nengsih

Cocoa seeds are included in recalcitrant seeds that have the characteristic of aging and regrowth of seeds during storage, viability of seeds decreases when their water content is decreased (12-31%) and can not stand to be stored at low temperature and humidity. For the provision of quality seeds need to be mastered technology seed storage properly. The aim of this research is to get the proper packing technique in maintaining viability of cocoa seed (Theobroma cacao L) during storage. The research will be conducted from Pebruari to Juni 2018, at Batanghari University Basic Laboratory. The design used is the Randomized Complete environment with the treatment of cardboard packaging with several ventilation holes: P0 = cardboard without ventilation, P1 = 2% ventilation box, P2 = 4% ventilation box, P3 = 6% ventilation box and P4 = cardboard box 8 %. The observed variables were: percentage of moisture content, percentage of seeds germinated in storage, percentage of sprout gain after storage, germination rate after storage, percentage of moldy seeds in storage and identification mushrooms. To see the effect, F test was done at 5% level, then continued with Duncan test at ? 5%. The result of the research showed that the treatment of the packing ventilation had an effect on the percentage parameter of moldy and germinated seed in storage and seed viability and vigor. In the treatment between ventilation, did not show any difference, but economically recommended the use of ventilation by 8%. Until storage on day 12 of the treatment of packaging vents can withstand the fungus attack 17.19% compared to control. The amount of ventilation does not show ability to withstand viability and vigor after storage. The fungus identified in seed storage is Fusarium spp. and Aspergillus sppKeywords: Recalcitrant, viability, moisture content of seedsAbstrakBenih kakao termasuk benih rekalsitran yang mempunyai sifat mengalami penuaan dan kemunduran benih selama penyimpanan, viabilitas benih menurun apabila diturunkan kadar airnya (12-31%) dan tidak tahan disimpan pada suhu dan kelembaban rendah. Untuk pengadaan benih berkualitas perlu dikuasai teknologi penyimpanan benih secara tepat. Penelitian bertujuan untuk mendapatkan teknik pengemasan yang tepat dalam mempertahankan viabilitas benih kakao (Theobroma cacao. L) selama penyimpanan. Penelitian dilaksanakan pada bulan Pebruari sampai Juni 2018, di Laboratorium Dasar Universitas Batanghari. Rancangan yang digunakan adalah rancangan lingkungan Acak Lengkap dengan perlakuan kemasan kardus dengan beberapa lubang ventilasi yaitu : P0= kardus tanpa ventilasi, P1= kardus ventilasi 2%, P2= kardus ventilasi 4%, P3= kardus ventilasi 6 % dan P4= kardus ventilasi 8%. Peubah yang diamati adalah: Kadar air benih, persentase benih yang berkecambah dalam penyimpanan, persentase daya kecambah setelah penyimpanan, kecepatan berkecambah setelah penyimpanan, persentase benih berjamur dalam penyimpanan dan identifikasi jamur. Untuk melihat pengaruh perlakuan, dilakukan uji F pada taraf 5%, kemudian dilanjutkan dengan uji Duncan pada taraf ? 5%. Hasil penelitian menunjukkan bahwa perlakuan ventilasi pengemasan berpengaruh terhadap peubah persentase benih berjamur dan berkecambah dalam penyimpanan serta viabilitas dan vigor benih. Pada perlakuan antar ventilasi, tidak menunjukkan adanya perbedaan, akan tetapi secara ekonomis dianjurkan penggunaan ventilasi sebesar 8%. Sampai penyimpanan pada hari ke-12 perlakuan ventilasi kemasan dapat menahan serangan jamur 17,19% dibandingkan kontrol. Jumlah ventilasi tidak menunjukkan mampu menahan viabilitas dan vigor setelah penyimpanan. Jamur yang teridentifikasi dalam penyimpanan benih adalah Fusarium spp. dan Aspergillus spp.Kata kunci : Rekalsitran, viabilitas, kadar air benih


1999 ◽  
Vol 9 (1) ◽  
pp. 63-73 ◽  
Author(s):  
A. Mead ◽  
D. Gray

AbstractThe prediction of the viability of stored seeds is important both for the management of germplasm collections and for the management of commercial seed production and storage. The Ellis and Roberts model for seed viability during storage is examined, and an inadequacy of the model highlighted. A modification is proposed, based on the ‘control mortality’ probit model developed for insecticide bioassays, to take proper account of variation in initial viability. This new ‘control viability’ model, relating seed viability to storage time, is fitted to data from a carrot seed storage experiment and found to fit well for a range of storage environments. A relationship, similar to that proposed by Ellis and Roberts for the effects of storage conditions on the rate of loss of viability, is fitted to the estimated rates from this new model. Data from a second carrot seed storage experiment are used to validate this relationship.


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