scholarly journals Developing a Sufficient Protocol for the Enhancement of α-Glucosidase Inhibitory Activity by Urena lobata L. Aeroponic Hairy Roots Using Exogenous Factors, a Precursor, and an Elicitor

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 548
Author(s):  
Dai Minh Cao ◽  
Phuong Thi Bach Vu ◽  
Minh Thi Thanh Hoang ◽  
Anh Lan Bui ◽  
Phuong Ngo Diem Quach
Keyword(s):  
Hairy Roots ◽  
Inhibitory Activity ◽  
High Growth ◽  
Dry Weight ◽  
Potential Method ◽  
High Growth Rate ◽  
Individual Treatment ◽  
Root Induction ◽  
Herbal Plants ◽  
Positive Correlations

Aeroponics is considered as a potential method for the culture of herbal plants due to the high growth rate, quantity and quality enhancement of secondary metabolites, and substantial environmental progress associated with this method. The aim of this study was to develop a sufficient protocol for successful Urena lobata hairy root induction by Agrobacterium rhizogenes ATCC 15834, using a precursor and elicitor to enhance α-glucosidase inhibitory activity (GIA) of aeroponic hairy roots (AHRs) in greenhouse conditions. In this study, we found that the optimized procedure (10 min, Woody plant medium (WPM), 1/25 salt strength) had an outstanding effect with a reduction in the rooting time (RT), promotion of the rooting rate (RR), and increase in the fresh weight (FW) and dry weight (DW) compared with the original procedure (30 min, Murashige and Skoog (MS) medium, 1/25 salt strength) after 30 days of culture. The highest DW, GIA, flavonoid (FLA) and phenolic (PHEL) contents were observed for individual addition of 10 mM phenylalanine (PA) or 50 mM chitosan (CS) in the late exponential phase (eighth week) with 15 days of elicitation compared to the control AHRs. However, individual treatment was less effective than the combination of the two. Positive correlations among the GIA, FLA and PHEL indicate that AHRs accumulated phenolic compounds, leading to an increase in the GIA by a synergistic effect. In conclusion, the culture of Urena lobata AHRs with PA and CS is an efficient procedure to produce GIA material in greenhouse conditions.

Hairy Roots

2021 ◽  
pp. 219-252
Keyword(s):  
Secondary Metabolites ◽  
Hairy Root ◽  
Hairy Roots ◽  
High Growth ◽  
Root Culture ◽  
Hairy Root Culture ◽  
High Growth Rate ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Transformed Root Cultures

Agrobacterium rhizogenes induces hairy root disease in plants. The neoplastic (cancerous) roots produced by A. rhizogenes infection, when cultured in hormone free medium, show high growth rate and genetic stability. These genetically transformed root cultures can produce levels of secondary metabolites comparable to that of intact plants. Several elicitation methods can be used to further enhance the production and accumulation of secondary metabolites. Thus, hairy root culture offer promise for high production and productivity of valuable secondary metabolites in many plants. Hairy roots can also produce recombinant proteins from transgenic roots, and thereby hold immense potential for pharmaceutical industry. Hairy root cultures can be used to elucidate the intermediates and key enzymes involved in the biosynthesis of secondary metabolites, and for phytoremediation due to their abundant neoplastic root proliferation property. Various applications of hairy root cultures and potential problems associated with them are discussed in this chapter.

On Stabilization of 3C-SiC Using Low Off-Axis 6H-SiC Substrates

2012 ◽  
Vol 717-720 ◽  
pp. 193-196 ◽  
Author(s):  
Valdas Jokubavicius ◽  
Björn Lundqvist ◽  
Philip Hens ◽  
Rickard Liljedahl ◽  
Rositza Yakimova ◽  
...  
Keyword(s):  
Growth Temperature ◽  
Growth Process ◽  
High Growth ◽  
Potential Method ◽  
High Growth Rate ◽  
Heteroepitaxial Growth ◽  
Bulk Properties ◽  
Combined Use ◽  
Sublimation Growth

Heteroepitaxial growth of 3C-SiC on 0.8 and 1.2 degree off-oriented 6H-SiC substrates was studied using a sublimation growth process. The 3C-SiC layers were grown at high growth rates with layer thickness up to 300 µm. The formation and the quality of 3C-SiC are influenced by the off-orientation of the substrate, the growth temperature (studied temperature range from 1750 °C to 1850°C), and the growth ambient (vacuum at 5*10-5mbar and nitrogen at 5*10-1mbar). The largest domains of 3C-SiC and the lowest number of double positioning boundaries were grown using nitrogen ambient and the highest growth temperature. The combined use of low off-axis substrate and high growth rate is a potential method to obtain material with bulk properties.

scholarly journals Evaluating α-glucosidase inhibitory activity of hairy roots cultured from some plants in malvaceae family induced by Agrobacterium rhizogenes ATCC 15834

2020 ◽  
Vol 2 (6) ◽  
pp. 165-174
Author(s):  
Vu Thi Bach Phuong ◽  
Pham Thi Anh Hong ◽  
Quach Ngo Diem Phuong
Keyword(s):  
Type 2 Diabetes ◽  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Hairy Roots ◽  
Inhibitory Activity ◽  
Root Culture ◽  
Root Induction ◽  
Hibiscus Sabdariffa ◽  
Sida Acuta

One of the most effective methods for type 2 diabetes treatments is inhibition of enzyme α-glucosidase in the intestines to slow down the release of glucose from carbohydrates in the diet, reduce plasma glucose levels and prevent hyperglycemia after meals. Therefore, seeking α-glucosidase inhibitors used in the treatment of diabetes from plant is the attention of many scientists. Based on the potential of the hairy root culture technology in increasing valuable chemical compounds accumulating, this study aimed to induce hairy roots from six plants of the Malvaceae family including Urena lobata, Abutilon indicum, Hibiscus Sabdariffa, Hibiscus rosa-sinensis, Sida acuta, Sida rhombifolia, and screening which materials has the highest in α-glucosidase inhibitory activity. We have successfully induced hairy roots from six plant species by using the Agrobacterium rhizogenes ATCC 15834 strain. The highest rates of hairy root induction were observed in Hibiscus Sabdariffa and Urena lobata. The stable introduction of rolB and rolC genes to plant genomes was confirmed by PCR. Under liquid-shake culture conditions on MS medium, hairy roots of Hibiscus sabdariffa, Urena lobata and Sida acuta showed better development than other species, and therefore, they are selected for the study of α-glucosidase inhibitory activity. This study proved that Urena lobata was stronger in inhibiting α-glucosidase activity than other studied plants, with the IC50 value of 7.65 μg/ml. The results of this study demonstrated Urena lobata hairy root might be considered as a potential supply of medicinal plants for the treatment of type 2 diabetes.  

scholarly journals Comparison of Different Strains of Agrobacterium rhizogenes for Hairy Root Induction and Betulin and Betulinic Acid Production in Morus alba

2017 ◽  
Vol 12 (4) ◽  
pp. 1934578X1701200
Author(s):  
Chang Ha Park ◽  
Shicheng Zhao ◽  
Hyeon Ji Yeo ◽  
Ye Eun Park ◽  
Thanislas Bastin Baska ◽  
...  
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Hairy Roots ◽  
Betulinic Acid ◽  
Acid Production ◽  
Leaf Tissue ◽  
Dry Weight ◽  
Morus Alba ◽  
Root Induction ◽  
Different Strains

Three Agrobacterium rhizogenes strains were tested for their ability to transform the plant Morus alba L. and to induce production of the secondary metabolites betulin and betulinic acid. All the tested strains of A. rhizogenes (R1601, LBA9402 and R1000) were able to induce hairy root formation in leaf tissue explants. Strain LBA9402 had the highest rate of infection (92.7% ± 8.8%), whereas strain R1601 had the lowest rate (87.4% ± 9.3%). The highest number of hairy roots per explant (5.6 ± 0.5) and the greatest root length (2.4 ± 0.2 mm) were obtained with strain LBA9402. We also evaluated dry weight (a measure of growth) and betulin and betulinic acid production in hairy roots and found that the highest growth (167.8 ± 14.5 mg/flask) occurred after infection with strain LBA9402. Furthermore the highest production of betulin (5.4 ± 0.4 mg/g dry weight) and betulinic acid (2.3 ± 0.2 mg/g dry weight) was noted using strain LBA9402. Among three elicitors, yeast extract showed the highest induction of betulin production (8.7 ± 0.4 mg/g) and silver nitrate induced the highest yield of betulinic acid (4.1 ± 0.2 mg/g). Our study showed that A. rhizogenes strain LBA9402 was the most effective of the three tested strains for production of transformed root cultures and betulin and betulinic acid.

Relationship between growth rate and the development of hollow stem in broccoli

2009 ◽  
Vol 60 (10) ◽  
pp. 995 ◽  
Author(s):  
M. Boersma ◽  
A. J. Gracie ◽  
P. H. Brown
Keyword(s):  
Growth Rate ◽  
High Growth ◽  
Dry Weight ◽  
High Growth Rate ◽  
Planting Density ◽  
Boron Deficiency ◽  
Absolute Growth Rate ◽  
Absolute Growth ◽  
Possible Cause

Boron deficiency is widely accepted as the dominant cause of hollow stem in broccoli (Brassica oleracea L. var. italica Plenck), although high growth rate has also been mentioned in the literature as a possible cause of the disorder. In this study, we investigated the role of growth rate and boron in the development of hollow stem. Two experiments were conducted with broccoli cv. Marathon, using planting density to manipulate growth rate. In the first experiment, plants were grown at 3 planting densities (19 512, 32 520, and 69 686 plants/ha) and either supplemented or not supplemented with foliar boron applications (0.35 kg B/ha) on two occasions. In the second experiment, broccoli plants at 2 planting densities (32 520 and 100 000 plants/ha) were treated in factorial combination from inflorescence initiation onwards with complete trace element fertiliser (1.5% B), 57% light interception, and paclobutrazol (480 g a.i./ha). The incidence and severity of hollow stem were assessed using digital image analysis and were found to increase with plant spacing but were not affected by boron application. The probability of hollow stem occurring increased with absolute growth rate in the first trial, but not the second. Absolute growth rate, measured as dry weight accumulation, was suppressed independently of planting density by the shading treatment, but did not influence the incidence or severity of hollow stem. The application of paclobutrazol did not influence absolute growth rate but reduced the severity of hollow stem at the lowest planting density. Our data support the hypothesis that the rate and pattern of growth are involved in the development of hollow stem, and that a boron deficiency is not the major causal factor.

Hairy Roots

2022 ◽  
pp. 735-759
Author(s):  
Pradip Chandra Deka
Keyword(s):  
Secondary Metabolites ◽  
Hairy Root ◽  
Hairy Roots ◽  
High Growth ◽  
Root Culture ◽  
Hairy Root Culture ◽  
High Growth Rate ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Transformed Root Cultures

Agrobacterium rhizogenes induces hairy root disease in plants. The neoplastic (cancerous) roots produced by A. rhizogenes infection, when cultured in hormone free medium, show high growth rate and genetic stability. These genetically transformed root cultures can produce levels of secondary metabolites comparable to that of intact plants. Several elicitation methods can be used to further enhance the production and accumulation of secondary metabolites. Thus, hairy root culture offer promise for high production and productivity of valuable secondary metabolites in many plants. Hairy roots can also produce recombinant proteins from transgenic roots, and thereby hold immense potential for pharmaceutical industry. Hairy root cultures can be used to elucidate the intermediates and key enzymes involved in the biosynthesis of secondary metabolites, and for phytoremediation due to their abundant neoplastic root proliferation property. Various applications of hairy root cultures and potential problems associated with them are discussed in this chapter.

scholarly journals Growth, biomass and lipid productivity of a newly isolated tropical marine diatom, Skeletonema sp. UHO29, under different light intensities

2020 ◽  
Vol 21 (4) ◽  
Author(s):  
Indrayani Indrayani ◽  
Haslianti Haslianti ◽  
Asmariani Asmariani ◽  
Wellem Muskita ◽  
Abdul Muis Balubi
Keyword(s):  
Growth Rate ◽  
Light Intensity ◽  
High Growth ◽  
Biomass Productivity ◽  
Dry Weight ◽  
High Light Intensity ◽  
High Growth Rate ◽  
Lipid Productivity ◽  
Marine Diatom ◽  
Light Intensities

Abstract. Indrayani I, Haslianti H, Asmariani A, Muskita WH, Balubi M. 2020. Growth, biomass and lipid productivity of a newly isolated tropical marine diatom, Skeletonema sp. UHO29, under different light intensities. Biodiversitas 21: 1498-1503. Light is one of the important factors affecting growth and biochemical composition of microalgae. The aim of this study was to determine growth, biomass and lipid productivity of a newly isolated marine diatom, Skeletonema sp.UHO29 under different light intensities. The Skeletonema sp. was cultured in 300 mL conical flasks containing 150 mL of f/2 medium under three different light intensities (28, 60 and 110 μmol photons m-2 s-1 ) with three replications. The cultures were incubated under ambient room temperatures, and a cycle of 12 hours light and 12 hours dark, in batch modes for two weeks. The results showed that the highest specific growth rate (0.56 d-1) was achieved at the highest light intensity (110 μmol photons m-2 s-1) and the lowest (0.11 d-1) was obtained at the lowest light intensity (28 μmol photons m-2 s-1). The highest biomass yield (0.62 g L-1) and biomass productivity (0.34 g L-1 d-1) was achieved at the highest light intensity. However, the highest lipid yield (0.175 g L-1), lipid content (28.78% ash-free dry weight) and lipid productivity (0.067 g L-1 d-1) were achieved at medium light intensity. The alga prefers high light intensity for higher growth rate and biomass productivity but it produces more lipids at medium light intensity. This study indicated that the Skeletonema sp.UHO29 is a potential species for outdoor mass cultivation and is a promising microalgal strain for use as a biodiesel feedstock due to its high growth rate, high biomass and lipid productivity.

The Job Of Microfinance For The Advancement Of Self Help Groups In India

2019 ◽  
Vol 118 (8) ◽  
pp. 236-240
Author(s):  
Dr.R. Murugesan ◽  
M. Leelavathi ◽  
Dr. K. Ravindran
Keyword(s):  
High Growth ◽  
High Growth Rate ◽  
Entrepreneurial Skills ◽  
Self Help ◽  
The Poor ◽  
Total Food ◽  
Self Help Groups ◽  
Positive Step ◽  
Food Consumed

towards jumping from the category of developing economy to developed economy there is one big factor that stops and poses a hindrance in its path of advancement and that obstacle is termed as Poverty. The Indian economic policy focuses on a high growth rate along with a equal participation of the poor so that they avail the opportunities available in the market economy. And in order to ensure the participation of the poor it has become important for the country to create a platform where the poor can easily access the various financial products. Microfinance is one such strategy for inclusive growth. Microfinance can change the life of the poor though not completely but a reasonable change can be ensured. In different phases of life women play a crucial role despite the discrimination that is faced by them. But equality can be endowed to women by enhancing the entrepreneurial skills in them. This is possible through Self Help Groups (SHGs). In India women produce around 30% of the total food consumed but she gets only 10% of the property or wealth of the country. Development of women is inevitable for the development and growth of any economy. SHGs happen to be a positive step in this direction. Along with these mediums there should be a cheap and easy source of credit for them and Microfinance fulfills the requirement. This study aims to find the role of this strong medium of Microfinance in the advancement of SHGs in India

Growth, Opium Gum Yield, and Photoperiod Response in Five Opium Poppy (Papaver somniferum L.) Cultivars

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 481d-481
Author(s):  
Z. Wang ◽  
M.C. Acock ◽  
B. Acock
Keyword(s):  
Flowering Time ◽  
Dry Matter ◽  
Dry Weight ◽  
Papaver Somniferum ◽  
Opium Poppy ◽  
Photoperiod Response ◽  
Asian Countries ◽  
Critical Photoperiod ◽  
Growth Chambers ◽  
Positive Correlations

To develop models for estimating growth, flowering time and gum yield of opium poppy, we compared variability among five cultivars (T, L, B1, B2, B3) from different latitudes in three Southeast Asian countries. Variability in the relationships between gum yield, capsule volume, and dry weight was also examined. Plants were grown in six growth chambers at a 11-, 12-, 13-, 14-, 15-, or 16-h photoperiod (PP) with a 12-h 25/20 °C thermoperiod. The main capsule was lanced for opium gum at 10, 13, and 16 d after flowering (DAF). Plants were harvested at 21 DAF and separated into leaves, stems, and capsules. Flowering time for B2 was affected least by PP and B1 the most. Flowering times for B3, L, and T were similar across the range of PPs. All cultivars showed a significant increase in flowering time from 14 to 13 h PP. Cultivars that flowered late (such as B1) had greater biomass than those that flowered earlier. However, cultivars that flowered earlier (such as L) had more dry matter partitioned into capsule than late-flowering ones. B2, B3, and L had the highest gum yields while B1 had the lowest. Positive correlations were found between gum dry weight and capsule volume (or dry weight) for T and L, but no correlations were observed between these variables for B1, B2, and B3. Our results indicated that plant dry weight varied as much as 77% and flowering time varied up to 40% even though the critical photoperiod was the same for all cultivars. The ratio of gum yield to capsule dry weight were significantly different between B1 and T.

Exploring SiC Growth Limitation of Vapor-Liquid-Solid Mechanism when Using Two Different Carbon Precursors

2013 ◽  
Vol 740-742 ◽  
pp. 323-326
Author(s):  
Kassem Alassaad ◽  
François Cauwet ◽  
Davy Carole ◽  
Véronique Soulière ◽  
Gabriel Ferro
Keyword(s):  
Growth Rate ◽  
High Growth ◽  
High Growth Rate ◽  
Carbon Precursor ◽  
Growth Limitation ◽  
Vapor Liquid Solid ◽  
Partial Pressures ◽  
Vapor Liquid Solid Mechanism ◽  
Vls Growth ◽  
Vapor Liquid

Abstract. In this paper, conditions for obtaining high growth rate during epitaxial growth of SiC by vapor-liquid-solid mechanism are investigated. The alloys studied were Ge-Si, Al-Si and Al-Ge-Si with various compositions. Temperature was varied between 1100 and 1300°C and the carbon precursor was either propane or methane. The variation of layers thickness was studied at low and high precursor partial pressure. It was found that growth rates obtained with both methane and propane are rather similar at low precursor partial pressures. However, when using Ge based melts, the use of high propane flux leads to the formation of a SiC crust on top of the liquid, which limits the growth by VLS. But when methane is used, even at extremely high flux (up to 100 sccm), no crust could be detected on top of the liquid while the deposit thickness was still rather small (between 1.12 μm and 1.30 μm). When using Al-Si alloys, no crust was also observed under 100 sccm methane but the thickness was as high as 11.5 µm after 30 min growth. It is proposed that the upper limitation of VLS growth rate depends mainly on C solubility of the liquid phase.

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