scholarly journals Effect of Deterpenated Origanum majorana L. Essential Oil on the Physicochemical and Biological Properties of Chitosan/β-Chitin Nanofibers Nanocomposite Films

Polymers ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1507
Author(s):  
Rut Fernández-Marín ◽  
Muhammad Mujtaba ◽  
Demet Cansaran-Duman ◽  
Ghada Ben Salha ◽  
Mª Ángeles Andrés Sánchez ◽  
...  
Keyword(s):  
Essential Oil ◽  
Nanocomposite Film ◽  
Water Solubility ◽  
Fungal Growth ◽  
Biological Properties ◽  
Nanocomposite Films ◽  
High Concentration ◽  
Fungal Growth Inhibition ◽  
Origanum Majorana ◽  
Chitin Nanofibers

Herein, the effect of three deterpenated fractions from Origanum majorana L. essential oil on the physicochemical, mechanical and biological properties of chitosan/β-chitin nanofibers-based nanocomposite films were investigated. In general, the incorporation of Origanum majorana L. original essential oil or its deterpenated fractions increases the opacity of the nanocomposite films and gives them a yellowish color. The water solubility decreases from 58% for chitosan/β-chitin nanofibers nanocomposite film to around 32% for the nanocomposite films modified with original essential oil or its deterpenated fractions. Regarding the thermal stability, no major changes were observed, and the mechanical properties decreased. Interestingly, data show differences on the biological properties of the materials depending on the incorporated deterpenated fraction of Origanum majorana L. essential oil. The nanocomposite films prepared with the deterpenated fractions with a high concentration of oxygenated terpene derivatives show the best antifungal activity against Aspergillus niger, with fungal growth inhibition of around 85.90%. Nonetheless, the only nanocomposite film that does not present cytotoxicity on the viability of L929 fibroblast cells after 48 and 72 h is the one prepared with the fraction presenting the higher terpenic hydrocarbon content (87.92%). These results suggest that the composition of the deterpenated fraction plays an important role in determining the biological properties of the nanocomposite films.

scholarly journals Corn Starch-Chitosan Nanocomposite Film Containing Nettle Essential Oil Nanoemulsions and Starch Nanocrystals: Optimization and Characterization

Polymers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 2113
Author(s):  
Fatemeh Kalateh-Seifari ◽  
Shima Yousefi ◽  
Hamed Ahari ◽  
Seyed Hedayat Hosseini
Keyword(s):  
Essential Oil ◽  
Nanocomposite Film ◽  
Food Packaging ◽  
Water Solubility ◽  
Corn Starch ◽  
Desirability Function ◽  
Nanocomposite Films ◽  
Function Technique ◽  
Vapor Permeability ◽  
Starch Nanocrystals

In the current study, nanocomposite films were produced based on corn starch:chitosan (CS:CH) biopolymers and the films were reinforced with nettle essential oil nanoemulsions (NEONEs) and starch nanocrystals (SNCs) to improve their physicochemical and mechanical properties. CS: CH at 70:30, 50:50, and 30:70 (w/w) ratios; SNCs at 2, 4, and 6% (w/w), and NEONEs at 0.5, 1, and 1.5% (w/w) were selected as variables. Then the various physical and mechanical attributes of chitosan-starch blended film containing SNCs and NEONEs were optimized using response surface methodology. The desirability function technique for the second-order polynomial models revealed that the following results could be achieved as the optimized treatment: water solubility of 51.56%; water absorption capacity of 128.75%; surface color of L (89.60), a (0.96), and b (1.90); water vapor permeability of 0.335 g/s Pa m, oxygen permeability of 2.60 cm3 μm/m2 d kPa; thickness of 154.41 µm, elongation at break of 53.54%; and tensile strength of 0.20 MPa at CS:CH of 38:62, SNC of 6.0%, and NEONEs of 0.41%. The nanocomposite film obtained can be employed as a novel biofunctional film with boosted physical mechanical and physical characteristics for food packaging applications.

scholarly journals Evaluation of Clausena anisata Essential Oil from Cameroon for Controlling Food Spoilage Fungi and its Potential Use as an Antiradical Agent

2011 ◽  
Vol 6 (9) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Aoudou Yaouba ◽  
Léopold Ngoune Tatsadjieu ◽  
Pierre Michel Jazet Dongmo ◽  
François Xavier Etoa ◽  
Carl Moses Fontum Mbofung ◽  
...  
Keyword(s):  
Essential Oil ◽  
Antiradical Activity ◽  
Fungal Growth ◽  
Diffusion Method ◽  
Germacrene D ◽  
Food Spoilage Fungi ◽  
Spoilage Fungi ◽  
Main Components ◽  
Fungal Growth Inhibition ◽  
Potential Use

Investigations were conducted to determine the chemical composition, antifungal and antiradical activities of the essential oil extracted from the fresh leaves of Clausena anisata (Willd.) Hook. F. ex Benth (from Cameroon) against Aspergillus flavus, A. niger, A. parasiticus and Fusarium moniliforme. The essential oil obtained by hydrodistillation was analysed by GC and GC/MS. The disc diffusion method was used to evaluate the fungal growth inhibition at various concentrations of the oil while the antiradical activity of the essential oil was studied by the DPPH (diphenyl picryl hydrazyl) method. The main components obtained were E-ocimenone (15.1%), Z-ocimenone (11.5%), γ-terpinene (11.4%) and germacrene D (10.9%). After 10 days of incubation on essential oil supplemented medium, the growth of A. flavus, A. niger, A. parasiticus and F. moniliforme were totally inhibited by 4, 5, 5 and 5 mg/mL of C. anisata essential oil, respectively. The antiradical activity of C. anisata essential oil (SC50 = 5.1 g/L) was less than that of butylated hydroxyl toluene (BHT), which was used as the reference compound (SC50 = 0.007 g/L). Results obtained in the present study indicate the possibility of exploiting C. anisata essential oil to fight strains of A. flavus, A. niger, A. parasiticus and F. moniliforme responsible for biodeterioration of stored food products.

scholarly journals EXTRAÇÃO, CARACTERIZAÇÃO QUÍMICA E ATIVIDADE ANTIFÚNGICA DE ÓLEO ESSENCIAL Syzygium aromaticum (CRAVO DA ÍNDIA)

2013 ◽  
Author(s):  
Vanessa Louzeiro Ascenção ◽  
Victor Elias Mouchrek Filho
Keyword(s):  
Essential Oil ◽  
Economic Value ◽  
Fungal Growth ◽  
Chemical Characterization ◽  
Gas Chromatography Mass Spectrometry ◽  
Major Constituent ◽  
Syzygium Aromaticum ◽  
Flower Buds ◽  
Pharmaceutical Industries ◽  
Fungal Growth Inhibition

A espécie vegetal Syzygium aromaticum, conhecida popularmente como cravo-da-índia, pertence à família das Mirtaceae e é explorada principalmente para extração industrial do óleo essencial obtido a partir dos botões florais, folhas e outras partes. Seu fruto contém um óleo essencial de grande valor econômico no mercado, devido ao elevado teor de eugenol (seu composto majoritário) o qual é largamente usado nas indústrias químicas e farmacêuticas. Neste trabalho, promoveu-se a extração do óleo essencial dos botões florais secos de Syzygium aromaticum através do método da hidrodestilação, utilizando um sistema de Clevenger modificado. Além das constantes físicas densidade, índice de refração, solubilidade, cor e aparência, foi possível, através da técnica de cromatografia gasosa/espectrometria de massas, identificar os componentes do óleo essencial e o eugenol como constituinte majoritário do óleo  essencial de Syzygium aromaticum, com 52,53%. Ao óleo essencial deu-se a aplicação fungicida, em que foi estudado o efeito antifúngico do óleo essencial de cravo da índia frente aos fungos F. oxysporum  f. sp. vasinfectum, F. oxysporum f. sp. passiflorae, F. oxysporum f. sp. lycopercisi, F. oxysporum f. sp. Tracheiphilum. Obtiveram-se ótimos resultados, já que a inibição de crescimento fúngico chegou a ser total em dois dos fungos estudados e entre 85% e 90% nos outros dois. Esse efeito justifica-se em razão do eugenol (componente majoritário do óleo) ter ação antifúngica comprovada.Palavras-chave: Óleo essencial. Cravo-da-índia. Atividade antifúngica. EXTRACTION, CHEMICAL CHARACTERIZATION AND ANTIFUNGAL ACTIVITY OF ESSENTIAL OIL Syzygium aromaticum (CLOVE)Abstract: The plant species Syzygium aromaticum, popularly known as clove, belongs to the family of Mirtaceae and operated primarily for industrial extraction of essential oil obtained from the flower buds, leaves and other parts. Its fruit contains an essential oil of great economic value in the market due to the high content of eugenol (its major compound) which is widely used in chemical and pharmaceutical industries. In this paper, we promoted the extraction of essential oil from dried flower buds of Syzygium aromaticum through the method of hydrodistillation using a modified Clevenger system. Besides the physical constants density, refractive index, solubility, color and appearance, was possible using the technique of gas chromatography / mass spectrometry to identify the components of the essential oil and eugenol as major constituent of the essential oil of Syzygium aromaticum, with 52.53 %. When essential oil gave up the fungicide application, in which we studied the antifungal effect of the essential oil of clove front fungi F. oxysporum f. sp. vasinfectum, F. oxysporum f. sp. passiflorae, F. oxysporum f. sp. lycopercisi, F. oxysporum f. sp. Tracheiphilum. There were obtained excellent results since fungal growth inhibition became total of two fungi studied and between 85% and 90% in the other two. This effect excused on account of eugenol (major component of the oil) have proven antifungal effect.Keywords: Essential oil. Clove. Antifungal activity.EXTRACCIÓN, CARACTERIZACIÓN QUÍMICA Y ACTIVIDAD ANTIFÚNGICA DE ACEITE ESENCIAL Syzygium aromaticum (clavo)Resumen: La especie vegetal Syzygium aromaticum, popularmente conocida como clavo de olor, pertenece a la familia Mirtaceae y es explotada principalmente para la extracción industrial de aceite esencial obtenido de los capullos de las flores, las hojas y otras partes. Su fruto contiene un aceite esencial de gran valor económico en el mercado debido al alto contenido de eugenol (su compuesto principal) que es ampliamente utilizado enla industria química y farmacéutica. En este trabajo, hemos promovido la extracción de aceite esencial de capullos secos de Syzygium aromaticum a través del método de hidrodestilación utilizando un sistema Clevenger modificado. Además de las constantes físicas, densidad, índice de refracción, solubilidad, color y apariencia, fue posible, usando la técnica de cromatografía de gases / espectrometría de masas identificar los componentes del aceite esencial y el eugenol como componente principal del aceite esencial de Syzygium aromaticum, con 52,53%. Al aceite esencial se le atribuyó la acitividad de fungicida, en el que se estudió el efecto antifúngico del aceite esencial de clavo frente a hongos F. oxysporum f. sp. vasinfectum, F. oxysporum f. sp. passiflorae, F. oxysporum f. sp. lycopercisi, F. oxysporum f. sp. Tracheiphilum. Se obtuvieron excelentes resultados ya quela inhibición del crecimiento fúngico fue total en dos de los hongos estudiados y entre 85% y 90% en los otros dos. Este efecto es explicado por cuenta de eugenol (componente principal del aceite) que tiene un efecto antifúngico comprobado.Palabras clave: Aceite esencial. Clavo de olor. La actividad antifúngica.

scholarly journals Potential use of lemongrass essential oil as fungicide against Aspergillus brasiliensis and as post-harvest protectant of wheat

2021 ◽  
Vol 43 ◽  
pp. e56763
Author(s):  
Lundoi Tobias Lee ◽  
Ana Paula Martinazzo ◽  
Carlos Eduardo de Souza Teodoro ◽  
Pedro Amorim Berbert
Keyword(s):  
Essential Oil ◽  
Fungal Growth ◽  
Alternative Methods ◽  
In Vitro Test ◽  
Wheat Grain ◽  
Oil Composition ◽  
Aspergillus Brasiliensis ◽  
Fungal Growth Inhibition ◽  
Lemongrass Essential Oil

Fungi are among the main responsible for damage and loss in stored grains, its control has been done through synthetic substances, which are harmful to man and the environment. Brazil, one of the leading countries in agriculture, has optimal environmental conditions for the development of mycotoxigenic fungi. Most of the synthetic chemicals used as preservatives have often been realized to be toxic to humans and also cause adverse environmental effects. Thus, it is necessary to search for alternative methods of controlling. In this study the aimed was to evaluate the efficacy of lemongrass essential oil in the control of the Aspergillus brasiliensis. In vitro and serial microdilution tests were carried out at different concentrations of essential oil and citral, which corresponds to 72% of the total oil composition. Inhibition of fungal growth on contaminated wheat grain was evaluated. The in vitro test results showed that the essential oil has fungicidal potential at concentrations from 0.6 μL mL-1, the minimum inhibitory concentration was determined at 0.8 μL mL-1. The tests with citral showed fungal control at concentrations from 0.6 μL mL-1 onwards. For wheat grain, fungal growth inhibition was obtained at the concentration of 1.6 μL mL-1. The essential oil of Cymbopogon flexuosus showed fungicidal activity against the fungus Aspergillus brasiliensis.

Fungal Growth Inhibition by Cheese Prepared Using Milk-clotting Crude Enzymes from the Edible Mushroom Hericium erinaceum

2020 ◽  
Vol 26 (1) ◽  
pp. 93-99
Author(s):  
Munekazu Kishimoto ◽  
Kazuo Nakamura ◽  
Takuto Tasaki ◽  
Kinya Matsumoto ◽  
Rina Nakano ◽  
...  
Keyword(s):  
Growth Inhibition ◽  
Fungal Growth ◽  
Edible Mushroom ◽  
Milk Clotting ◽  
Hericium Erinaceum ◽  
Fungal Growth Inhibition

Physicochemical Characterization and Antinociceptive Effect of β-cyclodextrin/Lippia pedunculosa Essential Oil in Mice

2018 ◽  
Vol 18 (9) ◽  
pp. 797-807 ◽  
Author(s):  
Paula dos Passos Menezes ◽  
Francielly de Oliveira Araujo ◽  
Tatianny Araujo Andrade ◽  
Igor Araujo Santos Trindade ◽  
Heitor Gomes de Araujo-Filho ◽  
...  
Keyword(s):  
Essential Oil ◽  
Physicochemical Properties ◽  
Water Solubility ◽  
Therapeutic Potential ◽  
Biological Activities ◽  
Antinociceptive Effect ◽  
Physicochemical Characterization ◽  
Good Tool ◽  
Main Challenge ◽  
Higher Temperature

Background: Some research studies have shown that Lippia pedunculosa essential oil (EOLP) has interesting biological activities. However, its low water solubility is the main challenge to achieve its therapeutic potential. In this context, Cyclodextrins (CDs) have been widely used in order to overcome this problem due to your capability to improve the physicochemical properties of drugs. Objective: In this perspective, the main goal of this study was to investigate how the improvement of the physicochemical properties of inclusion complexes (EOLP and β-CD) enhance the antinociceptive effect in mice. Methods: To achieve that, we prepared samples by Physical Mixture (PM), Paste Complexation (PC) and Slurry Complexation (SC) methods, followed by their physicochemical characterization. In addition, it was evaluated if the use of β-CD enhances the antinociceptive effect of EOLP in mice. Results: The analysis showed that rotundifolone (72.02%) was the major compound of EOLP and we found out based on DSC results that β-CD protected it from oxidation. In addition, TG techniques demonstrated that the best inclusion methods were PC and SC, due to their greater weight loss (10.8 and 11.6%, respectively) in the second stage (171-312°C), indicating that more complexed oil was released at the higher temperature than oil free. Other characteristics, such as changes in the typical crystalline form, and reduced particle size were observed by SEM and laser diffraction, respectively. The SC was the most effective complexation method, once the presence of rotundifolone was detected by FTIR. Based on that, SC method was used in all mice tests. In this regard, the number of paw licks was reduced for both compounds (all doses), but EOLP was more effective in reducing the nociceptive behavior. Conclusion: Therefore, CDs seem not to be a good tool to enhance the pharmacological properties of EOs rich in peroxide compounds such as rotundifolone.

Therapeutic Potential of Citronella Essential Oil: A Review

2019 ◽  
Vol 16 (4) ◽  
pp. 330-339 ◽  
Author(s):  
Ruchi Sharma ◽  
Rekha Rao ◽  
Sunil Kumar ◽  
Sheefali Mahant ◽  
Sarita Khatkar
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Environmental Protection Agency ◽  
Therapeutic Potential ◽  
Biological Properties ◽  
Toxic Encephalopathy ◽  
Practical Applications ◽  
Insect Repellents ◽  
Citronella Oil ◽  
Medicinal Value

Mosquito-borne diseases such as malaria, filariasis, chikunguniya, yellow fever, dengue and Japanese encephalitis are the major cause of remarkable morbidity and mortality in livestock and humans worldwide. Since ancient times, aromatic plants are used for their medicinal value. Essential oils derived from these plants may be used as effective alternatives/adjuvants in pharmaceuticals, biomedical, cosmetic, food, veterinary and agriculture applications. These oils have also gained popularity and interest for prevention and treatment of various disorders. However, several reports on adverse effects including skin eruption, contact artricaria or toxic encephalopathy in children are available for synthetic repellent in the literature. Thus, natural insect repellents like essential oils have been explored recently as an alternative. One such essential oil studied widely, is citronella oil, extracted mainly from Cymbopogon nardus. This essential oil has exhibited good efficacy against mosquitoes. It is a mixture of components including citronellal, citronellol, geraniol as major constituents contributing to various activities (antimicrobial, anthelmintic, antioxidant, anticonvulsant antitrypanosomal and wound healing), besides mosquito repellent action. Citronella essential oil is registered in US EPA (Environmental protection agency) as insect repellent due to its high efficacy, low toxicity and customer satisfaction. However, poor stability in the presence of air and high temperature limits its practical applications. Since specific knowledge on properties and chemical composition of oil is fundamental for its effective application, the present review compiles and discusses biological properties of citronella oil. It also sheds light on various formulations and applications of this essential oil.

scholarly journals Evaluation of Seven Essential Oils as Seed Treatments against Seedborne Fungal Pathogens of Cucurbita maxima

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2354
Author(s):  
Marwa Moumni ◽  
Mohamed Bechir Allagui ◽  
Kaies Mezrioui ◽  
Hajer Ben Amara ◽  
Gianfranco Romanazzi
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Fungal Pathogens ◽  
Seedling Emergence ◽  
Cucurbita Maxima ◽  
Melaleuca Alternifolia ◽  
Seedborne Pathogens ◽  
Origanum Majorana ◽  
Potential Use

Essential oils are gaining interest as environmentally friendly alternatives to synthetic fungicides for management of seedborne pathogens. Here, seven essential oils were initially tested in vivo for disinfection of squash seeds (Cucurbita maxima) naturally contaminated by Stagonosporopsis cucurbitacearum, Alternaria alternata, Fusarium fujikuro, Fusarium solani, Paramyrothecium roridum, Albifimbria verrucaria, Curvularia spicifera, and Rhizopus stolonifer. The seeds were treated with essential oils from Cymbopogon citratus, Lavandula dentata, Lavandula hybrida, Melaleuca alternifolia, Laurus nobilis, and Origanum majorana (#1 and #2). Incidence of S. cucurbitacearum was reduced, representing a range between 67.0% in L. nobilis to 84.4% in O. majorana #2. Treatments at 0.5 mg/mL essential oils did not affect seed germination, although radicles were shorter than controls, except with C. citratus and O. majorana #1 essential oils. Four days after seeding, seedling emergence was 20%, 30%, and 10% for control seeds and seeds treated with C. citratus essential oil (0.5 mg/mL) and fungicides (25 g/L difenoconazole plus 25 g/L fludioxonil). S. cucurbitacearum incidence was reduced by ~40% for plantlets from seeds treated with C. citratus essential oil. These data show the effectiveness of this essential oil to control the transmission of S. cucurbitacearum from seeds to plantlets, and thus define their potential use for seed decontamination in integrated pest management and organic agriculture.

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