Cellulose Acetate-Based Electrospun Materials with a Variety of Biological Potentials: Antibacterial, Antifungal and Anticancer

Novel eco-friendly fibrous materials with complex activities from cellulose acetate and cellulose acetate/polyethylene glycol (CA,PEG) containing 5-chloro-8-hydroxyquinoline as a model drug were obtained by electrospinning. Several methods, including scanning electron microscopy, X-ray diffraction analysis, ultraviolet-visible spectroscopy, water contact angle measurements, and mechanical tests, were utilized to characterize the obtained materials. The incorporation of PEG into the fibers facilitated the drug release. The amounts of the released drug from CA/5-Cl8Q and CA,PEG/5-Cl8Q were 78 ± 3.38% and 86 ± 3.02%, respectively (for 175 min). The antibacterial and antifungal activities of the obtained materials were studied. The measured zones of inhibition of CA/5-Cl8Q and CA,PEG/5-Cl8Q mats were 4.0 ± 0.18 and 4.5 ± 0.2 cm against S. aureus and around 4.0 ± 0.15 and 4.1 ± 0.22 cm against E. coli, respectively. The complete inhibition of the C. albicans growth was detected. The cytotoxicity of the obtained mats was tested toward HeLa cancer cells, SH-4 melanoma skin cells, and mouse BALB/c 3T3 fibroblasts as well. The CA/5-Cl8Q and CA,PEG/5-Cl8Q materials exhibited anticancer activity and low normal cell toxicity. Thus, the obtained fibrous materials can be suitable candidates for wound dressing applications and for application in local cancer treatment.

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Electrospun 5-chloro-8-hydroxyquinoline-Loaded Cellulose Acetate/Polyethylene Glycol Antifungal Membranes Against Esca

Polymers ◽

10.3390/polym11101617 ◽

2019 ◽

Vol 11 (10) ◽

pp. 1617 ◽

Cited By ~ 2

Author(s):

Spasova ◽

Manolova ◽

Rashkov ◽

Naydenov

Keyword(s):

Polyethylene Glycol ◽

Cellulose Acetate ◽

Plant Protection ◽

Bioactive Compound ◽

Mechanical Tests ◽

Fibrous Materials ◽

Water Contact ◽

Phaeomoniella Chlamydospora ◽

Optical And Mechanical Properties ◽

Surface Morphologies

Esca is one of the earliest described diseases in grapevines and causes trunk damage and the sudden wilting of the entire plant; it is caused mainly by the species Phaeomoniella chlamydospora (P. chlamydospora) and Phaeoacremonium aleophilum (P. aleophilum). In practice, there are no known curative approaches for fighting esca directly, which is a huge problem for preserving vineyards. Micro- and nanofibrous membranes from cellulose acetate (CA) and cellulose acetate/polyethylene glycol (CA/PEG) containing 5-chloro-8-hydroxyquinolinol (5-Cl8Q) were successfully prepared by electrospinning. The surface morphologies and optical and mechanical properties of the membranes were characterized by using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), ultraviolet-visible spectroscopy (UV-Vis), water contact angle measurements and mechanical tests. It was found that the bioactive compound release was facilitated by PEG. The antifungal activities of the obtained materials against P. chlamydospora and P. aleophilum were studied. We have demonstrated that 5-Cl8Q is an efficient and sustainable antifungal agent against P. chlamydospora and P. aleophilum. Moreover, for the first time, the present study reveals the possibility of using electrospun polymer membranes containing 5-Cl8Q which impede the penetration and growth of P. chlamydospora and P. aleophilum. Thus, the obtained fibrous materials can be suitable candidates for plant protection against diverse fungi.

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PLA-Based Hybrid and Composite Electrospun Fibrous Scaffolds as Potential Materials for Tissue Engineering

Journal of Nanomaterials ◽

10.1155/2017/9246802 ◽

2017 ◽

Vol 2017 ◽

pp. 1-11 ◽

Cited By ~ 9

Author(s):

Anna Magiera ◽

Jarosław Markowski ◽

Elzbieta Menaszek ◽

Jan Pilch ◽

Stanislaw Blazewicz

Keyword(s):

Tissue Engineering ◽

Mechanical Tests ◽

Hybrid Structures ◽

Poly Lactic Acid ◽

Electrospinning Technique ◽

Water Contact ◽

Human Osteoblasts ◽

Angle Measurements ◽

Contact Angle Measurements ◽

Normal Human

The aim of the study was to manufacture poly(lactic acid)- (PLA-) based nanofibrous nonwovens that were modified using two types of modifiers, namely, gelatin- (GEL-) based nanofibres and carbon nanotubes (CNT). Hybrid nonwovens consisting of PLA and GEL nanofibres (PLA/GEL), as well as CNT-modified PLA nanofibres with GEL nanofibres (PLA + CNT/GEL), in the form of mats, were manufactured using concurrent-electrospinning technique (co-ES). The ability of such hybrid structures as potential scaffolds for tissue engineering was studied. Both types of hybrid samples and one-component PLA and CNTs-modified PLA mats were investigated using scanning electron microscopy (SEM), water contact angle measurements, and biological and mechanical tests. The morphology, microstructure, and selected properties of the materials were analyzed. Biocompatibility and bioactivity in contact with normal human osteoblasts (NHOst) were studied. The coelectrospun PLA and GEL nanofibres retained their structures in hybrid samples. Both types of hybrid nonwovens were not cytotoxic and showed better osteoinductivity in comparison to scaffolds made from pure PLA. These samples also showed significantly reduced hydrophobicity compared to one-component PLA nonwovens. The CNT-contained PLA nanofibres improved mechanical properties of hybrid samples and such a 3D system appears to be interesting for potential application as a tissue engineering scaffold.

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Nanostructured Polymer Brushes With Reversibly Changing Properties

MRS Proceedings ◽

10.1557/proc-727-r2.5 ◽

2002 ◽

Vol 727 ◽

Author(s):

Denys Usov ◽

Manfred Stamm ◽

Sergiy Minko ◽

Christian Froeck ◽

Andreas Scholl ◽

...

Keyword(s):

Polymer Brushes ◽

Phase Segregation ◽

Water Contact ◽

Angle Measurements ◽

Nanostructured Polymer ◽

Contact Angle Measurements ◽

Vertical Segregation ◽

Oxygen Plasma Etching ◽

Grafting From ◽

Photoemission Electron

AbstractWe investigated the interplay between different mechanisms of the lateral and vertical segregation in the synthesized via “grafting from” approach symmetric A/B (where A and B are poly(styrene-co-2,3,4,5,6-pentafluorostyrene) and poly(methylmethacrylate), respectively) polymer brushes upon exposure to different solvents. We used X-ray photoemission electron spectroscopy and microscopy (X-PEEM), AFM, water contact angle measurements, and oxygen plasma etching to study morphology of the brushes. The ripple morphology after toluene (nonselective solvent) revealed elongated lamellar-like domains of A and B polymers alternating across the surface. The dimple-A morphology consisting of round clusters of the polymer A was observed after acetone (selective solvent for B). The top layer was enriched with the polymer B showing that the brush underwent both the lateral and vertical phase segregation. A qualitative agreement with predictions of SCF theory was found.

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Synthesis, characterization and biological activities of 1,3,4-oxadiazole derivatives of nalidixic acid and their copper complexes

10.31221/osf.io/g7mx8 ◽

2019 ◽

Author(s):

Chem Int

Keyword(s):

Nalidixic Acid ◽

Copper Complexes ◽

Biological Activities ◽

Bidentate Coordination ◽

Antibacterial And Antifungal Activities ◽

Elemental Analyses ◽

Oxadiazole Derivatives ◽

Antibacterial And Antifungal ◽

Derivatives Of ◽

Substituted 1

A series of novel 1, 3, 4-oxadiazole analogues was synthesized from cyclization of hydrazones of substituted 1-ethyl-1,4-dihydro-7-methyl-4-oxo-1,8-naphthyridine-3-carbohydrazides were prepared from nalidixic acid. The structures of synthesized oxadiazole derivatives and their copper complexes were elucidated on the basis of FTIR, elemental analyses, 1H-NMR and atomic absorption spectral analysis. It was observed from spectral data that metal ligand ratio was 1:1 in all copper complexes and they were bidentate, coordination was found to be done through oxygen of 4-oxo group and nitrogen of oxadiazole ring. The synthesized compounds were further evaluated with biological activities and compared with parent hydrazones. Copper complexes possess antibacterial and antifungal activities better than the oxadiazoles while they have better antioxidant activity then copper complexes. Parent hydrazones were better in all biological activities than synthesized oxadiazoles.

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Substituted 4-Formyl-2H-chromen-2-ones: Their Reaction with N-(2,3,4,6-Tetra-Oacetyl- β-D-galactopyranosyl)thiosemicarbazide, Antibacterial and Antifungal Activity of Their Thiosemicarbazone Products

Current Organic Chemistry ◽

10.2174/1385272824999200812132256 ◽

2020 ◽

Vol 24 (19) ◽

pp. 2272-2282

Author(s):

Vu Ngoc Toan ◽

Nguyen Minh Tri ◽

Nguyen Dinh Thanh

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Candida Albicans ◽

Selenium Dioxide ◽

Antibacterial And Antifungal Activity ◽

E Coli ◽

Antibacterial And Antifungal Activities ◽

Alkyl Ethers ◽

Antibacterial And Antifungal

Several 6- and 7-alkoxy-2-oxo-2H-chromene-4-carbaldehydes were prepared from corresponding alkyl ethers of 6- and 7-hydroxy-4-methyl-2-oxo-2H-chromen-2-ones by oxidation using selenium dioxide. 6- and 7-Alkoxy-4-methyl-2H-chromenes were obtained with yields of 57-85%. Corresponding 4-carbaldehyde derivatives were prepared with yields of 41-67%. Thiosemicarbazones of these aldehydes with D-galactose moiety were synthesized by reaction of these aldehydes with N-(2,3,4,6-tetra-O-acetyl-β-Dgalactopyranosyl) thiosemicarbazide with yields of 62-74%. These thiosemicarbazones were screened for their antibacterial and antifungal activities in vitro against bacteria, such as Staphylococcus aureus, Escherichia coli, and fungi, such as Aspergillus niger, Candida albicans. Several compounds exhibited strong inhibitory activity with MIC values of 0.78- 1.56 μM, including 8a (against S. aureus, E. coli, and C. albicans), 8d (against E. coli and A. niger), 9a (against S. aureus), and 9c (against S. aureus and C. albicans).

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Synthesis & Anticancer Evaluation of New Substituted 2-(3,4- Dimethoxyphenyl)benzazoles

Medicinal Chemistry ◽

10.2174/1573406414666180711130012 ◽

2019 ◽

Vol 15 (3) ◽

pp. 287-297 ◽

Cited By ~ 1

Author(s):

Cigdem Karaaslan ◽

Yalcin Duydu ◽

Aylin Ustundag ◽

Can O. Yalcin ◽

Banu Kaskatepe ◽

...

Keyword(s):

Hela Cells ◽

Parp Inhibitor ◽

A549 Cells ◽

Human Tumor ◽

Antimicrobial Activities ◽

Sodium Metabisulfite ◽

Human Tumor Cell Lines ◽

Antibacterial And Antifungal Activities ◽

Antibacterial And Antifungal ◽

Mitotic Inhibitor

Background: The benzazole nucleus is found in many promising small molecules such as anticancer and antibacterial agents. Bendamustine (Alkylating agent), Nocodazole (Mitotic inhibitor), Veliparib (PARP inhibitor), and Glasdegib (SMO inhibitor) are being clinically used as anticancer therapeutic which bear benzimidazole moiety. Based on the principle of bioisosterism, in the present work, 23 compounds belonging to 2-(3,4-dimethoxyphenyl)benzazoles and imidazopyridine series were synthesized and evaluated for their anticancer and antimicrobial activities. Objective: A series of new 2-(3,4-dimethoxyphenyl)-1H-benz(or pyrido)azoles were synthesized and evaluated for their anticancer and antimicrobial activities. Method: N-(5-chloro-2-hdroxyphenyl)-3,4-dimethoxybenzamide 1, was obtained by the amidation of 2-hydroxy-5-chloroaniline with 3,4-dimethoxybenzoic acid by using 1,1&'-carbonyldiimidazole. Cyclization of 1 to benzoxazole derivative 2, was achieved by p-toluenesulfonic acid. Other 1H-benz(or pyrido)azoles were prepared by the reaction between 2-aminothiophenol, ophenylenediamine, o-pyridinediamine with sodium metabisulfite adduct of 3,4-dimethoxybenzaldehyde. The NMR assignments of the dimethoxy groups were established by the NOESY spectra. Results: Compound 12, bearing two chlorine atoms at the 5(4) and 7(6) positions of the benzene moiety of benzimidazole was found the most potent analogue against A549 cells with the GI50 value of 1.5 μg/mL. Moreover, 24 showed remarkable cell growth inhibition against MCF-7 and HeLa cells with the GI50 values of 7 and 5.5 μg/mL, respectively. The synthesized compounds have no important antibacterial and antifungal activities. Conclusion: It could be concluded that the introduction of di-chloro atoms at the phenyl ring of 2-(3,4-dimethoxyphenyl)-1H-benzimidazoles increases significant cytotoxicity to selected human tumor cell lines in comparison to other all benzazoles synthesized. Unsubstituted 2-(3,4- dimethoxyphenyl)-imidazopyridines also gave good inhibitory profile against A549 and HeLa cells.

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On the Effectiveness of Oxygen Plasma and Alkali Surface Treatments to Modify the Properties of Polylactic Acid Scaffolds

Polymers ◽

10.3390/polym13101643 ◽

2021 ◽

Vol 13 (10) ◽

pp. 1643

Author(s):

Ricardo Donate ◽

María Elena Alemán-Domínguez ◽

Mario Monzón

Keyword(s):

Surface Properties ◽

Enzymatic Degradation ◽

Oxygen Plasma ◽

Physicochemical Characterization ◽

Surface Treatments ◽

Carboxyl Groups ◽

Scanning Calorimetry ◽

Water Contact ◽

Angle Measurements ◽

3D Printed

Surface modification of 3D-printed PLA structures is a major issue in terms of increasing the biofunctionality and expanding the tissue engineering applications of these parts. In this paper, different exposure times were used for low-pressure oxygen plasma applied to PLA 3D-printed scaffolds. Alkali surface treatments were also evaluated, aiming to compare the modifications introduced on the surface properties by each strategy. Surface-treated samples were characterized through the quantification of carboxyl groups, energy-dispersive X-ray spectroscopy, water contact angle measurements, and differential scanning calorimetry analysis. The change in the surface properties was studied over a two-week period. In addition, an enzymatic degradation analysis was carried out to evaluate the effect of the surface treatments on the degradation profile of the 3D structures. The physicochemical characterization results suggest different mechanism pathways for each type of treatment. Alkali-treated scaffolds showed a higher concentration of carboxyl groups on their surface, which enhanced the enzymatic degradation rate, but were also proven to be more aggressive towards 3D-printed structures. In contrast, the application of the plasma treatments led to an increased hydrophilicity of the PLA surface without affecting the bulk properties. However, the changes on the properties were less steady over time.

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Synthesis of the Bacteriostatic Poly(L-Lactide) by Using Zinc (II)[(acac)(L)H2O] (L = Aminoacid-Based Chelate Ligands) as an Effective ROP Initiator

International Journal of Molecular Sciences ◽

10.3390/ijms22136950 ◽

2021 ◽

Vol 22 (13) ◽

pp. 6950

Author(s):

Renata Barczyńska-Felusiak ◽

Małgorzata Pastusiak ◽

Piotr Rychter ◽

Bożena Kaczmarczyk ◽

Michał Sobota ◽

...

Keyword(s):

Schiff Bases ◽

Antibacterial Properties ◽

Antimicrobial Effect ◽

Zinc Complexes ◽

Antibacterial And Antifungal Activities ◽

Aspergillus Brasiliensis ◽

Acetylacetonate Complex ◽

Distorted Trigonal Bipyramid ◽

Composition Structure ◽

Antibacterial And Antifungal

The paper presents a synthesis of poly(l-lactide) with bacteriostatic properties. This polymer was obtained by ring-opening polymerization of the lactide initiated by selected low-toxic zinc complexes, Zn[(acac)(L)H2O], where L represents N-(pyridin-4-ylmethylene) tryptophan or N-(2-pyridin-4-ylethylidene) phenylalanine. These complexes were obtained by reaction of Zn[(acac)2 H2O] and Schiff bases, , the products of the condensation of amino acids and 4-pyridinecarboxaldehyde. The composition, structure, and geometry of the synthesized complexes were determined by NMR and FTIR spectroscopy, elemental analysis, and molecular modeling. Both complexes showed the geometry of a distorted trigonal bipyramid. The antibacterial and antifungal activities of both complexes were found to be much stronger than those of the primary Schiff bases. The present study showed a higher efficiency of polymerization when initiated by the obtained zinc complexes than when initiated by the zinc(II) acetylacetonate complex. The synthesized polylactide showed antibacterial properties, especially the product obtained by polymerization initiated by a zinc(II) complex with a ligand based on l-phenylalanine. The polylactide showed a particularly strong antimicrobial effect against Pseudomonas aeruginosa, Staphylococcus aureus, and Aspergillus brasiliensis. At the same time, this polymer does not exhibit fibroblast cytotoxicity.

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Epoxy/alumina composite coating on welded steel 316L with excellent wear and anticorrosion properties

Scientific Reports ◽

10.1038/s41598-021-91741-y ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Maryama Hammi ◽

Younes Ziat ◽

Zakaryaa Zarhri ◽

Charaf Laghlimi ◽

Abdelaziz Moutcine

Keyword(s):

Electrochemical Behaviour ◽

Sem Analysis ◽

Water Contact ◽

Welded Steel ◽

Melted Zone ◽

Angle Measurements ◽

Contact Angle Measurements ◽

Alumina Composite ◽

The Cost ◽

Good Contrast

AbstractThe main purpose of this study is to elaborate anticorrosive coatings for the welded steel 316L, since this later is widely used in industrial field. Hence, within this work we have studied the electrochemical behaviour of different zones of the welded steel 316 in 1 M HCl media. The macrography study of the welded steel has revealed the different areas with a good contrast. We have stated three different zones, namely; melted zone (MZ), heat affected zone (HAZ) and base metal zone (BM). Impedance studies on welded steel 316L were conducted in 1 M HCl solution, coating of Epoxy/Alumina composite was applied on different zones, in order to reveal the anti-corrosion efficiency in each zone. Scanning electron microscopy (SEM) analysis was undertaken in order to check how far the used coating in such aggressive media protects the studied zones and these findings were assessed by water contact angle measurements. The choice of this coating is based on the cost and the safety. We concluded that the Epoxy/Alumina composite has a good protecting effect regarding welded steel in aggressive media.

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Valorization of Sicanaodorifera (Vell.) Naudin Epicarp as a Source of Bioactive Compounds: Chemical Characterization and Evaluation of Its Bioactive Properties

Foods ◽

10.3390/foods10040700 ◽

2021 ◽

Vol 10 (4) ◽

pp. 700

Author(s):

Bianca R. Albuquerque ◽

Maria Inês Dias ◽

Carla Pereira ◽

Jovana Petrović ◽

Marina Soković ◽

...

Keyword(s):

Phenolic Compounds ◽

Bioactive Compounds ◽

Food Additives ◽

Chemical Characterization ◽

Dry Weight ◽

Bioactive Molecules ◽

High Concentration ◽

Minimal Inhibitory Concentrations ◽

Antibacterial And Antifungal Activities ◽

Antibacterial And Antifungal

Fruit bio-residues can be interesting for the recovery of bioactive molecules, such as phenolic compounds, tocopherols, vitamins, among others. These compounds can be targeted at the food industry and used for the development of functional foods or as food additives. In some cases, fruit epicarps are converted into by-products with non-commercial value, and generally, these fruit parts have a higher content in bioactive compounds than the fruit pulp. From this perspective, S. odorifera, a Brazilian fruit, has an inedible epicarp that could be explored to obtain biological compounds. Therefore, the aims of this study were to evaluate the chemical composition and the antioxidant, anti-proliferative, anti-inflammatory, and antimicrobial bioactivities of this by-product. S. odorifera epicarp showed a total of four organic acids, four phenolic compounds, highlighting the high concentration of anthocyanins (24 ± 1 mg/g dry weight (dw)) and high content of tocopherols (366 ± 2 mg/100 g dw). The hydroethanolic extract showed considerable antioxidant activity (EC50 values of 48.2 ± 0.5 and 27 ± 1 µg/mL for TBARS and OxHLIA assays, respectively), as also antibacterial and antifungal activities (minimal inhibitory concentrations (MICs) ≤ 2.2 mg/mL). The results obtained in this study suggest that Sicana odorifera epicarp represents a reliable option for the development of novel natural-based colorants with functional/bioactive proprieties.

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