scholarly journals Image-Based Phenotyping of Flowering Intensity in Cool-Season Crops

Sensors ◽  
2020 ◽  
Vol 20 (5) ◽  
pp. 1450 ◽  
Author(s):  
Chongyuan Zhang ◽  
Wilson Craine ◽  
Rebecca McGee ◽  
George Vandemark ◽  
James Davis ◽  
...  

The timing and duration of flowering are key agronomic traits that are often associated with the ability of a variety to escape abiotic stress such as heat and drought. Flowering information is valuable in both plant breeding and agricultural production management. Visual assessment, the standard protocol used for phenotyping flowering, is a low-throughput and subjective method. In this study, we evaluated multiple imaging sensors (RGB and multiple multispectral cameras), image resolution (proximal/remote sensing at 1.6 to 30 m above ground level/AGL), and image processing (standard and unsupervised learning) techniques in monitoring flowering intensity of four cool-season crops (canola, camelina, chickpea, and pea) to enhance the accuracy and efficiency in quantifying flowering traits. The features (flower area, percentage of flower area with respect to canopy area) extracted from proximal (1.6–2.2 m AGL) RGB and multispectral (with near infrared, green and blue band) image data were strongly correlated (r up to 0.89) with visual rating scores, especially in pea and canola. The features extracted from unmanned aerial vehicle integrated RGB image data (15–30 m AGL) could also accurately detect and quantify large flowers of winter canola (r up to 0.84), spring canola (r up to 0.72), and pea (r up to 0.72), but not camelina or chickpea flowers. When standard image processing using thresholds and unsupervised machine learning such as k-means clustering were utilized for flower detection and feature extraction, the results were comparable. In general, for applicability of imaging for flower detection, it is recommended that the image data resolution (i.e., ground sampling distance) is at least 2–3 times smaller than that of the flower size. Overall, this study demonstrates the feasibility of utilizing imaging for monitoring flowering intensity in multiple varieties of evaluated crops.

Molecules ◽  
2019 ◽  
Vol 24 (7) ◽  
pp. 1235 ◽  
Author(s):  
Jianying Yuan ◽  
Dequan Guo ◽  
Gexiang Zhang ◽  
Prithwineel Paul ◽  
Ming Zhu ◽  
...  

Image edge detection is a fundamental problem in image processing and computer vision, particularly in the area of feature extraction. However, the time complexity increases squarely with the increase of image resolution in conventional serial computing mode. This results in being unbearably time consuming when dealing with a large amount of image data. In this paper, a novel resolution free parallel implementation algorithm for gradient based edge detection, namely EDENP, is proposed. The key point of our method is the introduction of an enzymatic numerical P system (ENPS) to design the parallel computing algorithm for image processing for the first time. The proposed algorithm is based on a cell-like P system with a nested membrane structure containing four membranes. The start and stop of the system is controlled by the variables in the skin membrane. The calculation of edge detection is performed in the inner three membranes in a parallel way. The performance and efficiency of this algorithm are evaluated on the CUDA platform. The main advantage of EDENP is that the time complexity of O ( 1 ) can be achieved regardless of image resolution theoretically.


Author(s):  
Klaus-Ruediger Peters

Differential hysteresis processing is a new image processing technology that provides a tool for the display of image data information at any level of differential contrast resolution. This includes the maximum contrast resolution of the acquisition system which may be 1,000-times higher than that of the visual system (16 bit versus 6 bit). All microscopes acquire high precision contrasts at a level of <0.01-25% of the acquisition range in 16-bit - 8-bit data, but these contrasts are mostly invisible or only partially visible even in conventionally enhanced images. The processing principle of the differential hysteresis tool is based on hysteresis properties of intensity variations within an image.Differential hysteresis image processing moves a cursor of selected intensity range (hysteresis range) along lines through the image data reading each successive pixel intensity. The midpoint of the cursor provides the output data. If the intensity value of the following pixel falls outside of the actual cursor endpoint values, then the cursor follows the data either with its top or with its bottom, but if the pixels' intensity value falls within the cursor range, then the cursor maintains its intensity value.


Author(s):  
B. Roy Frieden

Despite the skill and determination of electro-optical system designers, the images acquired using their best designs often suffer from blur and noise. The aim of an “image enhancer” such as myself is to improve these poor images, usually by digital means, such that they better resemble the true, “optical object,” input to the system. This problem is notoriously “ill-posed,” i.e. any direct approach at inversion of the image data suffers strongly from the presence of even a small amount of noise in the data. In fact, the fluctuations engendered in neighboring output values tend to be strongly negative-correlated, so that the output spatially oscillates up and down, with large amplitude, about the true object. What can be done about this situation? As we shall see, various concepts taken from statistical communication theory have proven to be of real use in attacking this problem. We offer below a brief summary of these concepts.


Entropy ◽  
2021 ◽  
Vol 23 (7) ◽  
pp. 816
Author(s):  
Pingping Liu ◽  
Xiaokang Yang ◽  
Baixin Jin ◽  
Qiuzhan Zhou

Diabetic retinopathy (DR) is a common complication of diabetes mellitus (DM), and it is necessary to diagnose DR in the early stages of treatment. With the rapid development of convolutional neural networks in the field of image processing, deep learning methods have achieved great success in the field of medical image processing. Various medical lesion detection systems have been proposed to detect fundus lesions. At present, in the image classification process of diabetic retinopathy, the fine-grained properties of the diseased image are ignored and most of the retinopathy image data sets have serious uneven distribution problems, which limits the ability of the network to predict the classification of lesions to a large extent. We propose a new non-homologous bilinear pooling convolutional neural network model and combine it with the attention mechanism to further improve the network’s ability to extract specific features of the image. The experimental results show that, compared with the most popular fundus image classification models, the network model we proposed can greatly improve the prediction accuracy of the network while maintaining computational efficiency.


2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Dominik Jens Elias Waibel ◽  
Sayedali Shetab Boushehri ◽  
Carsten Marr

Abstract Background Deep learning contributes to uncovering molecular and cellular processes with highly performant algorithms. Convolutional neural networks have become the state-of-the-art tool to provide accurate and fast image data processing. However, published algorithms mostly solve only one specific problem and they typically require a considerable coding effort and machine learning background for their application. Results We have thus developed InstantDL, a deep learning pipeline for four common image processing tasks: semantic segmentation, instance segmentation, pixel-wise regression and classification. InstantDL enables researchers with a basic computational background to apply debugged and benchmarked state-of-the-art deep learning algorithms to their own data with minimal effort. To make the pipeline robust, we have automated and standardized workflows and extensively tested it in different scenarios. Moreover, it allows assessing the uncertainty of predictions. We have benchmarked InstantDL on seven publicly available datasets achieving competitive performance without any parameter tuning. For customization of the pipeline to specific tasks, all code is easily accessible and well documented. Conclusions With InstantDL, we hope to empower biomedical researchers to conduct reproducible image processing with a convenient and easy-to-use pipeline.


2007 ◽  
Vol 15 (3) ◽  
pp. 137-151 ◽  
Author(s):  
Hua Ma ◽  
Carl A. Anderson

A critical parameter in the evaluation of pharmaceutical dosage forms by hyperspectral imaging is the level of magnification. If the magnification (as set by the optical objective) is inadequate to resolve the relevant features, then the value of the imaging is diminished; if the magnification level is greater than is required, then the field of view is unnecessarily reduced. The purpose of this study was to determine an optimum magnification level for the study of powder mixing. Relevant features in this system include distribution of individual components within samples and the overall content of a given sample. In the present study, three magnification levels of near infrared (NIR) chemical imaging objectives were evaluated for their effects on imaging a blend of pharmaceutical materials (powders). High, medium and low objective magnification levels were investigated by comparing the resulting blend surface images of a two-component (salicylic acid and lactose) pharmaceutical powder mixture. Multiple images from high and medium magnification were concatenated so that an equivalent field of view was obtained for all magnification levels. Univariate images, principal component analysis score images, partial least squares predicted images and spectra extracted from different intensity regions in the area images were analysed qualitatively and quantitatively for comparison. A series of images spanning a strip across the centre of the circular field were collected at each magnification level and compared with respect to surface features elucidated and area of blend surface imaged. Analyses of images indicate that the three magnification levels delineate the component distribution for this particular powder system similarly. Images obtained at the low magnification level demonstrated adequate resolution and provided the broadest view of the blend surface. It is concluded that the low optical magnification level was adequate for the system being studied and is the preferred mode for pharmaceutical powder blend image data collection for this system.


2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Zhaolin Lu ◽  
Xiaojuan Hu ◽  
Yao Lu

Particle morphology, including size and shape, is an important factor that significantly influences the physical and chemical properties of biomass material. Based on image processing technology, a method was developed to process sample images, measure particle dimensions, and analyse the particle size and shape distributions of knife-milled wheat straw, which had been preclassified into five nominal size groups using mechanical sieving approach. Considering the great variation of particle size from micrometer to millimeter, the powders greater than 250 μm were photographed by a flatbed scanner without zoom function, and the others were photographed using a scanning electron microscopy (SEM) with high-image resolution. Actual imaging tests confirmed the excellent effect of backscattered electron (BSE) imaging mode of SEM. Particle aggregation is an important factor that affects the recognition accuracy of the image processing method. In sample preparation, the singulated arrangement and ultrasonic dispersion methods were used to separate powders into particles that were larger and smaller than the nominal size of 250 μm. In addition, an image segmentation algorithm based on particle geometrical information was proposed to recognise the finer clustered powders. Experimental results demonstrated that the improved image processing method was suitable to analyse the particle size and shape distributions of ground biomass materials and solve the size inconsistencies in sieving analysis.


2018 ◽  
Vol 30 (03) ◽  
pp. 1850024 ◽  
Author(s):  
Zeinab Heidari ◽  
Mehrdad Dadgostar ◽  
Zahra Einalou

Breast cancer is one of the main causes of women’s death. Thermal breast imaging is one the non-invasive method for cancer at early stage diagnosis. In contrast to mammography this method is cheap and painless and it can be used during pregnancy while ionized beams are not used. Specialists are seeking new ways to diagnose the cancer in early stages. Segmentation of the breast tissue is one of the most indispensable stages in most of the cancer diagnosis methods. By the advancement of infrared precise cameras, new and fast computers and nouvelle image processing approaches, it is feasible to use thermal imaging for diagnosis of breast cancer at early stages. Since the breast form is different in individuals, image segmentation is a hard task and semi-automatic or manual methods are usual in investigations. In this research the image data base of DMR-IR has been utilized and a now automatic approach has been proposed which does not need learning. Data were included 159 gray images used by dynamic protocol (132 healthy and 27 patients). In this study, by combination of different image processing methods, the segmentation of thermal images of the breast tissues have been completed automatically and results show the proper performance of recommended method.


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