scholarly journals An Analysis of a Compact Label-Free Guiding-Wave Biosensor Based on a Semiconductor-Clad Dielectric Strip Waveguide

Sensors ◽  
2020 ◽  
Vol 20 (12) ◽  
pp. 3368 ◽  
Author(s):  
Carlos Angulo Barrios
Keyword(s):  
Limit Of Detection ◽  
Beam Propagation Method ◽  
Three Dimensional ◽  
Silicon Oxynitride ◽  
Practical Implementation ◽  
Label Free ◽  
Sensor Performance ◽  
Strip Waveguide ◽  
Fabrication Tolerances ◽  
Photonic Biosensors

In this paper, a compact, integrated, semiconductor-clad strip waveguide label-free biosensor is proposed and analyzed. The device is based on CMOS-compatible materials such as amorphous-Si and silicon oxynitride. The optical sensor performance has been modeled by a three-dimensional beam propagation method. The simulations indicate that a 20-μm-long device can exhibit a surface limit of detection of 3 ng/cm2 for avidin molecules in aqueous solution. The sensor performance compares well to those displayed by other photonic biosensors with much larger footprints. The fabrication tolerances have been also studied in order to analyze the feasibility of the practical implementation of the biosensor.

scholarly journals Electro-Oxidation and Simultaneous Determination of Indole-3-Acetic Acid and Salicylic Acid on Graphene Hydrogel Modified Electrode

Sensors ◽  
2019 ◽  
Vol 19 (24) ◽  
pp. 5483 ◽  
Author(s):  
Xiaodong Cao ◽  
Xueting Zhu ◽  
Shudong He ◽  
Xuan Xu ◽  
Yongkang Ye
Keyword(s):  
Acetic Acid ◽  
Salicylic Acid ◽  
Modified Electrode ◽  
Limit Of Detection ◽  
Simultaneous Detection ◽  
Three Dimensional ◽  
Linear Sweep Voltammetry ◽  
Oxidation Reactions ◽  
Sensor Performance ◽  
Indole 3 Acetic Acid

A selective and sensitive electrochemical sensor was developed for simultaneous detection of phytohormones indole-3-acetic acid (IAA) and salicylic acid (SA). The sensing interface was fabricated on a porous, three-dimensional networked graphene hydrogel (GH) modified glassy carbon electrode (GCE). The electrocatalytic behavior of IAA and SA on the surface of the modified electrode (GH/GCE) was investigated by cyclic voltammetry and linear sweep voltammetry. Results show that the oxidation reactions of IAA and SA occur at different potentials, which enable their simultaneous detection at the sensing interface. Under optimal conditions, the GH/GCE exhibited good selectivity and stability and its response, unaffected by various interferents, was linear in the range of 4 to 200 μM of IAA and SA. The limit of detection (S/N = 3) achieved were 1.42 μM for IAA and 2.80 μM for SA. The sensor performance was validated by measuring for IAA and SA in real vegetable samples with satisfactory results.

A method for autonomous collision-free navigation of a quadrotor UAV in unknown tunnel-like environments

Robotica ◽  
2021 ◽  
pp. 1-27
Author(s):  
Taha Elmokadem ◽  
Andrey V. Savkin
Keyword(s):  
Unmanned Aerial Vehicles ◽  
Autonomous Underwater Vehicles ◽  
Three Dimensional ◽  
Underwater Vehicles ◽  
Practical Implementation ◽  
Challenging Problem ◽  
Quadrotor Uav ◽  
Navigation Algorithm ◽  
Aerial Vehicles ◽  
Quadrotor Uavs

Abstract Unmanned aerial vehicles (UAVs) have become essential tools for exploring, mapping and inspection of unknown three-dimensional (3D) tunnel-like environments which is a very challenging problem. A computationally light navigation algorithm is developed in this paper for quadrotor UAVs to autonomously guide the vehicle through such environments. It uses sensors observations to safely guide the UAV along the tunnel axis while avoiding collisions with its walls. The approach is evaluated using several computer simulations with realistic sensing models and practical implementation with a quadrotor UAV. The proposed method is also applicable to other UAV types and autonomous underwater vehicles.

An acoustic liner design methodology based on a statistical source model

2021 ◽  
pp. 1475472X2110238
Author(s):  
Douglas M Nark ◽  
Michael G Jones
Keyword(s):  
Design Methodology ◽  
Three Dimensional ◽  
Aircraft Engine ◽  
Flight Test ◽  
Source Model ◽  
Practical Implementation ◽  
Source Information ◽  
Fan Noise ◽  
Future Design ◽  
Acoustic Liner

The attenuation of fan tones remains an important aspect of fan noise reduction for high bypass ratio turbofan engines. However, as fan design considerations have evolved, the simultaneous reduction of broadband fan noise levels has gained interest. Advanced manufacturing techniques have also opened new possibilities for the practical implementation of broadband liner concepts. To effectively address these elements, practical acoustic liner design methodologies must provide the capability to efficiently predict the acoustic benefits of novel liner configurations. This paper describes such a methodology to design and evaluate multiple candidate liner configurations using realistic, three dimensional geometries for which minimal source information is available. The development of the design methodology has been guided by a series of studies culminating in the design and flight test of a low drag, broadband inlet liner. The excellent component and system noise benefits obtained in this test demonstrate the effectiveness of the broadband liner design process. They also illustrate the value of the approach in concurrently evaluating multiple liner designs and their application to various locations within the aircraft engine nacelle. Thus, the design methodology may be utilized with increased confidence to investigate novel liner configurations in future design studies.

scholarly journals Demonstration of a Label-Free and Low-Cost Optical Cavity-Based Biosensor Using Streptavidin and C-Reactive Protein

Biosensors ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 4
Author(s):  
Donggee Rho ◽  
Seunghyun Kim
Keyword(s):  
Limit Of Detection ◽  
Point Of Care ◽  
Low Cost ◽  
Optical Cavity ◽  
Sample Volume ◽  
Small Sample ◽  
Label Free ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Cavity Structure

An optical cavity-based biosensor (OCB) has been developed for point-of-care (POC) applications. This label-free biosensor employs low-cost components and simple fabrication processes to lower the overall cost while achieving high sensitivity using a differential detection method. To experimentally demonstrate its limit of detection (LOD), we conducted biosensing experiments with streptavidin and C-reactive protein (CRP). The optical cavity structure was optimized further for better sensitivity and easier fluid control. We utilized the polymer swelling property to fine-tune the optical cavity width, which significantly improved the success rate to produce measurable samples. Four different concentrations of streptavidin were tested in triplicate, and the LOD of the OCB was determined to be 1.35 nM. The OCB also successfully detected three different concentrations of human CRP using biotinylated CRP antibody. The LOD for CRP detection was 377 pM. All measurements were done using a small sample volume of 15 µL within 30 min. By reducing the sensing area, improving the functionalization and passivation processes, and increasing the sample volume, the LOD of the OCB are estimated to be reduced further to the femto-molar range. Overall, the demonstrated capability of the OCB in the present work shows great potential to be used as a promising POC biosensor.

scholarly journals Flow Control in Porous Media: From Numerical Analysis to Quantitative μPAD for Ionic Strength Measurements

Sensors ◽  
2021 ◽  
Vol 21 (10) ◽  
pp. 3328
Author(s):  
Pouya Mehrdel ◽  
Hamid Khosravi ◽  
Shadi Karimi ◽  
Joan Antoni López Martínez ◽  
Jasmina Casals-Terré
Keyword(s):  
Porous Media ◽  
Limit Of Detection ◽  
Fluid Dynamic ◽  
Porous Membrane ◽  
External Pressure ◽  
Paper Substrate ◽  
Sensor Performance ◽  
Food Applications ◽  
Substrate Nature

Microfluidic paper-based analytical devices (µPADs) are a promising technology to enable accurate and quantitative in situ assays. Paper’s inherent hydrophilicity drives the fluids without the need for external pressure sources. However, controlling the flow in the porous medium has remained a challenge. This study addresses this problem from the nature of the paper substrate and its design. A computational fluid dynamic model has been developed, which couples the characteristics of the porous media (fiber length, fiber diameter and porosity) to the fluidic performance of the diffusion-based µPAD sensor. The numerical results showed that for a given porous membrane, the diffusion, and therefore the sensor performance is affected not only by the substrate nature but also by the inlets’ orientation. Given a porous substrate, the optimum performance is achieved by the lowest inlets’ angle. A diffusion-based self-referencing colorimetric sensor was built and validated according to the design. The device is able to quantify the hydronium concentration in wines by comparison to 0.1–1.0 M tartaric acid solutions with a 41.3 mM limit of detection. This research showed that by proper adjustments even the simplest µPADs can be used in quantitative assays for agri-food applications.

scholarly journals Surface Plasmon Resonance Assay for Label-Free and Selective Detection of HIV-1 p24 Protein

Biosensors ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 180
Author(s):  
Lucia Sarcina ◽  
Giuseppe Felice Mangiatordi ◽  
Fabrizio Torricelli ◽  
Paolo Bollella ◽  
Zahra Gounani ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Covalent Binding ◽  
Hiv Infections ◽  
Selectivity Ratio ◽  
Selective Detection ◽  
Label Free ◽  
Self Assembled Monolayer ◽  
Label Free Detection ◽  
P24 Protein ◽  
Hiv 1

The early detection of the human immunodeficiency virus (HIV) is of paramount importance to achieve efficient therapeutic treatment and limit the disease spreading. In this perspective, the assessment of biosensing assay for the HIV-1 p24 capsid protein plays a pivotal role in the timely and selective detection of HIV infections. In this study, multi-parameter-SPR has been used to develop a reliable and label-free detection method for HIV-1 p24 protein. Remarkably, both physical and chemical immobilization of mouse monoclonal antibodies against HIV-1 p24 on the SPR gold detecting surface have been characterized for the first time. The two immobilization techniques returned a capturing antibody surface coverage as high as (7.5 ± 0.3) × 1011 molecule/cm2 and (2.4 ± 0.6) × 1011 molecule/cm2, respectively. However, the covalent binding of the capturing antibodies through a mixed self-assembled monolayer (SAM) of alkanethiols led to a doubling of the p24 binding signal. Moreover, from the modeling of the dose-response curve, an equilibrium dissociation constant KD of 5.30 × 10−9 M was computed for the assay performed on the SAM modified surface compared to a much larger KD of 7.46 × 10−5 M extracted for the physisorbed antibodies. The chemically modified system was also characterized in terms of sensitivity and selectivity, reaching a limit of detection of (4.1 ± 0.5) nM and an unprecedented selectivity ratio of 0.02.

scholarly journals Label-Free Electrochemical Biosensor Based on Au@MoS₂–PANI for Escherichia coli Detection

Chemosensors ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 49
Author(s):  
Pushap Raj ◽  
Man Hwan Oh ◽  
Kyudong Han ◽  
Tae Yoon Lee
Keyword(s):  
Escherichia Coli ◽  
Bacterial Infections ◽  
Limit Of Detection ◽  
Bacterial Pathogens ◽  
Cost Effective ◽  
Covalent Immobilization ◽  
Label Free ◽  
Self Assembled Monolayer ◽  
Detection Range ◽  
Linear Detection

Bacterial infections have become a significant challenge in terms of public health, the food industry, and the environment. Therefore, it is necessary to address these challenges by developing a rapid, cost-effective, and easy-to-use biosensor for early diagnosis of bacterial pathogens. Herein, we developed a simple, label-free, and highly sensitive immunosensor based on electrochemical detection using the Au@MoS₂–PANI nanocomposite. The conductivity of the glassy carbon electrode is greatly enhanced using the Au@MoS₂–PANI nanocomposite and a self-assembled monolayer of mercaptopropionic acid on the gold nanoparticle surface was employed for the covalent immobilization of antibodies to minimize the nonspecific adsorption of bacterial pathogens on the electrode surface. The biosensor established a high selectivity and sensitivity with a low limit of detection of 10 CFU/mL, and detected Escherichia coli within 30 min. Moreover, the developed biosensor demonstrated a good linear detection range, practical utility in urine samples, and electrode regenerative studies.

scholarly journals Fluorescence Based on Surface Plasmon Coupled Emission for Ultrahigh Sensitivity Immunoassay of Cardiac Troponin I

Biomedicines ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 448
Author(s):  
Vien Thi Tran ◽  
Heongkyu Ju
Keyword(s):  
Surface Plasmon ◽  
Cardiac Troponin ◽  
Troponin I ◽  
Limit Of Detection ◽  
Delayed Diagnosis ◽  
Cardiac Troponin I ◽  
Quantitative Detection ◽  
Fluorescence Signal ◽  
Label Free ◽  
Ultrahigh Sensitivity

This work demonstrates the quantitative assay of cardiac Troponin I (cTnI), one of the key biomarkers for acute cardiovascular diseases (the leading cause of death worldwide) using the fluorescence-based sandwich immune reaction. Surface plasmon coupled emission (SPCE) produced by non-radiative coupling of dye molecules with surface plasmons being excitable via the reverse Kretschmann format is exploited for fluorescence-based sandwich immunoassay for quantitative detection of cTnI. The SPCE fluorescence chip utilizes the gold (2 nm)-silver (50 nm) bimetallic thin film, with which molecules of the dye Alexa 488 (conjugated with detection antibodies) make a near field coupling with the plasmonic film for SPCE. The experimental results find that the SPCE greatly improves the sensitivity via enhancing the fluorescence signal (up to 50-fold) while suppressing the photo-bleaching, permitting markedly enhanced signal-to-noise ratio. The limit of detection of 21.2 ag mL−1 (atto-gram mL−1) is obtained, the lowest ever reported to date amid those achieved by optical technologies such as luminescence and label-free optical sensing techniques. The features discovered such as ultrahigh sensitivity may prompt the presented technologies to be applied for early diagnosis of cTnI in blood, particularly for emergency medical centers overloaded with patients with acute myocardial infarction who would suffer from time-delayed diagnosis due to insufficient assay device sensitivity.

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