scholarly journals Release and Detection of microRNA by Combining Magnetic Hyperthermia and Electrochemistry Modules on a Microfluidic Chip

Sensors ◽  
2020 ◽  
Vol 21 (1) ◽  
pp. 185
Author(s):  
Marie-Charlotte Horny ◽  
Vincent Dupuis ◽  
Jean-Michel Siaugue ◽  
Jean Gamby
Keyword(s):  
Microfluidic Chip ◽  
Local Heating ◽  
Electrochemical Techniques ◽  
Magnetic Hyperthermia ◽  
Microrna Target ◽  
Ferrite Core ◽  
Shell Nanoparticles ◽  
Lab On Chip ◽  
Biological Target ◽  
On Chip

The heating of a biologic solution is a crucial part in an amplification process such as the catalytic detection of a biological target. However, in many situations, heating must be limited in microfluidic devices, as high temperatures can cause the denaturation of the chip components. Local heating through magnetic hyperthermia on magnetic nano-objects has opened the doors to numerous improvements, such as for oncology where a reduced heating allows the synergy of chemotherapy and thermotherapy. Here we report on the design and implementation of a lab on chip without global heating of samples. It takes advantage of the extreme efficiency of DNA-modified superparamagnetic core–shell nanoparticles to capture complementary sequences (microRNA-target), uses magnetic hyperthermia to locally release these targets, and detects them through electrochemical techniques using ultra-sensitive channel DNA-modified ultramicroelectrodes. The combination of magnetic hyperthermia and microfluidics coupled with on-chip electrochemistry opens the way to a drastic reduction in the time devoted to the steps of extraction, amplification and nucleic acids detection. The originality comes from the design and microfabrication of the microfluidic chip suitable to its insertion in the millimetric gap of toric inductance with a ferrite core.

From CAD to microfluidic chip within one day: rapid prototyping of lab-on-chip cartridges using generic polymer parts

2020 ◽  
Vol 30 (11) ◽  
pp. 115012 ◽  
Author(s):  
Daniel Podbiel ◽  
Lorenz Boecking ◽  
Hannah Bott ◽  
Julian Kassel ◽  
Daniel Czurratis ◽  
...  
Keyword(s):  
Rapid Prototyping ◽  
Microfluidic Chip ◽  
Lab On Chip ◽  
On Chip

scholarly journals Epitaxial Graphene Sensors Combined with 3D Printed Microfluidic Chip for Heavy Metals Detection

Proceedings ◽  
2018 ◽  
Vol 2 (13) ◽  
pp. 982 ◽  
Author(s):  
Maria Francesca Santangelo ◽  
Ivan Shtepliuk ◽  
Donatella Puglisi ◽  
Daniel Filippini ◽  
Rositsa Yakimova ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Microfluidic Chip ◽  
Chemical Interaction ◽  
High Sensitivity ◽  
Epitaxial Graphene ◽  
Phase Detection ◽  
Lab On Chip ◽  
Sensing Platform ◽  
3D Printed ◽  
On Chip

Two-dimensional materials may constitute key elements in the development of a sensing platform where extremely high sensitivity is required, since even minimal chemical interaction can generate appreciable changes in the electronic state of the material. In this work, we investigate the sensing performance of epitaxial graphene on Si-face 4H-SiC (EG/SiC) for liquid-phase detection of heavy metals (e.g., Pb). The integration of preparatory steps needed for sample conditioning is included in the sensing platform, exploiting fast prototyping using a 3D printer, which allows direct fabrication of a microfluidic chip incorporating all the features required to connect and execute the Lab-on-chip (LOC) functions. It is demonstrated that interaction of Pb2+ ions in water-based solutions with the EG enhances its conductivity exhibiting a Langmuir correlation between signal and Pb2+ concentration. Several concentrations of Pb2+ solutions ranging from 125 nM to 500 µM were analyzed showing good stability and reproducibility over time.

scholarly journals Fast Track DNA Analysis Suite for human identification

2013 ◽  
Author(s):  
Peter T Docker ◽  
Joanna Baker ◽  
Steve Haswell
Keyword(s):  
Microfluidic Chip ◽  
Fast Track ◽  
Dna Analysis ◽  
Human Identification ◽  
Rapid Analysis ◽  
Human Interaction ◽  
Lab On Chip ◽  
Human Dna ◽  
On Chip ◽  
Proof Of Principle

This paper details the development of a portable ‘Lab on chip’ DNA analyser that was developed to facilitate rapid analysis of DNA samples for ‘at scene of crime’ and in custody suite situations where human identification is required rapidly. This system was proven to work with human DNA for 3 loci, namely VWA, D21 and D18 taken from raw sample through PCR separation to detection within 90miniutes. Once the sample was loaded onto the microfluidic chip which in turn was loaded into the instrument no further human interaction took place. This paper details the approach to the biochemistry, hardware before going on to give results proving the proof of principle and then the authors’ conclusions.

Integrated Microfluidic Flow Sensor for LAB-oN-CHIP and PoINT-oF-CARE Applications

2020 ◽  
Vol 36 (4) ◽  
pp. 112-120
Author(s):  
A.V. Zverev ◽  
M. Andronik ◽  
V.V. Echeistov ◽  
Z.H. Issabayeva ◽  
O.S. Sorokina ◽  
...  
Keyword(s):  
Numerical Simulation ◽  
Flow Rate ◽  
Microfluidic Chip ◽  
Soft Lithography ◽  
Point Of Care ◽  
Flow Sensor ◽  
Oxide Semiconductor ◽  
Dead Volume ◽  
Lab On Chip ◽  
On Chip

The results of the development and manufacture of an integrated membrane-free sensor for the control of accurate dilution of liquid samples on the microfluidic chip are presented. The proposed type of devices is intended for direct precise measurements of liquid flow rate in microchannels of laboratories-on-chip, including point-of-care systems. The sensor topology was optimized based on the numerical simulation results and technological requirements. The main characteristic of the developed sensor is the lack of a membrane in the design while maintaining the sensitivity and accuracy of the device at the level of a commercial membrane analogue. The fully biocompatible sensor was manufactured using standard microelectronics and soft lithography technologies. In order to optimize the sensor design, 32 different topologies of the device were tested. The integration of the flow sensors on the chip allows to significantly reduce the dead volume of the hydrodynamic system and to control the amount of liquid entering the individual reservoirs of the microfluidic chip. The sensor occupies an area of (210 x 140) um2 in the channel and is characterized by a relative error of 5% in the flow rate range of 100-1000 ul/min. microfluidics, lab-on-chip, calorimetric flow sensor, thermoresistive sensor, numerical simulation, hydrodynamics, complementary metal-oxide-semiconductor, microtechnologies Devices were made at the BMSTU Nanofabrication Facility (FMN Laboratory, FMNS REC, ID 74300).

scholarly journals Fast Track DNA Analysis Suite for human identification

2013 ◽  
Author(s):  
Peter T Docker ◽  
Joanna Baker ◽  
Steve Haswell
Keyword(s):  
Microfluidic Chip ◽  
Fast Track ◽  
Dna Analysis ◽  
Human Identification ◽  
Rapid Analysis ◽  
Human Interaction ◽  
Lab On Chip ◽  
Human Dna ◽  
On Chip ◽  
Proof Of Principle

This paper details the development of a portable ‘Lab on chip’ DNA analyser that was developed to facilitate rapid analysis of DNA samples for ‘at scene of crime’ and in custody suite situations where human identification is required rapidly. This system was proven to work with human DNA for 3 loci, namely VWA, D21 and D18 taken from raw sample through PCR separation to detection within 90miniutes. Once the sample was loaded onto the microfluidic chip which in turn was loaded into the instrument no further human interaction took place. This paper details the approach to the biochemistry, hardware before going on to give results proving the proof of principle and then the authors’ conclusions.

INTEGRATED MICROFLUIDIC FLOW SENSOR FOR LAB-ON-CHIP AND POINT-OF-CARE APPLICATIONS

2020 ◽  
pp. 457-458
Author(s):  
V. Ryzhkov ◽  
M. Andronik ◽  
V. Echeistov ◽  
Z. Issabayeva ◽  
O. Sorokina ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Point Of Care ◽  
Experimental Comparison ◽  
Fluid Flow Rate ◽  
Fabrication Technology ◽  
Flow Sensors ◽  
Lab On Chip ◽  
Microfluidic Flow ◽  
Chip Fabrication ◽  
On Chip

An integrated membrane-free sensor for precise measurements of fluid flow rate in microchannels of laboratories-on- chip has been developed. The sensor allows to measure flow on microfluidic chip in real time and is designed for liquid samples precise dilution control on the microfluidic chip. Fabrication technology of the microfluidic chip with built-in flow sensors as well as results of experimental comparison of developed sensor with a commercial flowmeter are presented.

scholarly journals Design and Fabrication of Multichannel PDMS Microfluidic

2021 ◽  
Vol 2129 (1) ◽  
pp. 012048
Author(s):  
M N Afnan Uda ◽  
U Hashim ◽  
M N A Uda ◽  
N A Parmin ◽  
V Thivina
Keyword(s):  
Contact Area ◽  
Microfluidic Chip ◽  
Point Of Care ◽  
Main Tool ◽  
Morphological Properties ◽  
Single Chip ◽  
Lab On Chip ◽  
Micro Channels ◽  
On Chip ◽  
Disease Analysis

Abstract Microfluidic delivers miniaturized fluidic networks for processing liquids in the microliter range. In the recent years, lab-on-chip (LOC) is become a main tool for point-of-care (POC) diagnostic especially in the medical field. In this paper, we presented a design and fabrication on multi disease analysis using single chip via delivery of fluid with the multiple transducers is the pathway of multi-channel microfluidic based LOC’s. 3 in 1 nano biosensor kit was attached with the microfluidic to produce nano-biolab-on-chip (NBLOC). The multi channels microfluidic chip was designed including the micro channels, one inlet, three outlet and sensor contact area. The microfluidic chip was designed to include multiplex detection for pathogen that consists of multiple channels of simultaneous results. The LOC system was designed using Design Spark Mechanical software and PDMS was used as a medium of the microfluidic. The microfluidic mold and PDMS microfluidic morphological properties have been characterized by using low power microscope (LPM), high power microscope (HPM) and surface profiler. The LOC system physical was experimental by dropping food coloring through the inlet and collecting at the sensor contact area outlet.

scholarly journals Reusable Embedded Microcoils for Magnetic Nano-Beads Trapping in Microfluidics: Magnetic Simulation and Experiments

Micromachines ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 257 ◽  
Author(s):  
Olivier Lefebvre ◽  
Hong Ha Cao ◽  
Meritxell Cortés Francisco ◽  
Marion Woytasik ◽  
Elisabeth Dufour-Gergam ◽  
...  
Keyword(s):  
Power Consumption ◽  
Microfluidic Chip ◽  
Coating Layer ◽  
Adhesion Layer ◽  
Fe Method ◽  
New Approach ◽  
Lab On Chip ◽  
Highly Sensitive ◽  
On Chip ◽  
The Magnetic Field

In this study, a microfluidic chip with integrated coil was designed and fabricated for the aim of effectively trapping magnetic nanobeads (Adembeads®, 300 nm) and measuring the chip’s temperature during the working time. In addition, a reversible technique of bonding Polydimethylsiloxane (PDMS) channels was presented. This bonding process used a coating layer of CYTOP®product as a protection, insulation and low-adhesion layer. The reversible packaging technique allows the bottom substrate to be reused, possibly equipped with sensors, and to use a disposable microchannels network. The FE method was employed to calculate the magnetic field and power consumption by the ANSYS® version 12.1 software. Merit factors were defined in order to synthetically represent the ability of the simulated coil to trap beads for a unit power consumption, i.e. a given heat generation. The simulation results propose a new approach to optimize the design criteria in fabricating planar microcoils. The optimal microcoils were fabricated and then used to realize a magnetic immunoassay in a microfluidic chip. The aim was to integrate these microcoils into a lab-on-chip and obtain a fast and highly sensitive biological element detection.

scholarly journals Magnetic Hyperthermia on γ-Fe2O3@SiO2 Core-Shell Nanoparticles for mi-RNA 122 Detection

Nanomaterials ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 149
Author(s):  
Marie-Charlotte Horny ◽  
Jean Gamby ◽  
Vincent Dupuis ◽  
Jean-Michel Siaugue
Keyword(s):  
Biomedical Applications ◽  
Local Heating ◽  
Slight Modification ◽  
Silica Coating ◽  
Magnetic Hyperthermia ◽  
Core Shell ◽  
Shell Nanoparticles ◽  
Alternative Magnetic Field ◽  
Core Shell Nanoparticles ◽  
Global Heating

Magnetic hyperthermia on core-shell nanoparticles bears promising achievements, especially in biomedical applications. Here, thanks to magnetic hyperthermia, γ-Fe2O3 cores are able to release a DNA target mimicking the liver specific oncotarget miRNA-122. Our silica coated magnetic nanoparticles not only allow the grafting at their surface of a significant number of oligonucleotides but are also shown to be as efficient, by local heating, as 95 °C global heating when submitted to an alternative magnetic field, while keeping the solution at 28 °C, crucial for biological media and energy efficiency. Moreover, a slight modification of the silica coating process revealed an increased heating power, well adapted for the release of small oligonucleotides such as microRNA.

Sign in / Sign up

Export Citation Format

Share Document