Assessment of Water Buffalo Milk and Traditional Milk Products in a Sustainable Production System

Water buffalo (Bubalus bubalis) conservation in Serbia is under an in situ program, but additional efforts are needed to ensure the development of this animal’s genetic resources biodiversity. This research aims to describe challenges and possible strategies for sustainable water buffalo milk production. In this study, the physicochemical characteristics of buffalo milk and buffalo dairy products (cheese, butter, and kajmak) were determined. Furthermore, amino and fatty acids composition and the related health lipid indices (atherogenic and thrombogenic) were assessed. The findings support the fact that buffalo milk is a reliable source of high-quality nutrients (dry matter: 16.10%, fat: 6.02%, protein: 4.61%). Leucine, lysine, and valine content were found to be high in buffalo milk and cheese. A substantial quantity of non-essential glutamic and aspartic amino acids was observed in milk, as well as glutamic acid and tyrosine in cheese. It was established that milk protein of buffalo cheese had a favorable proportion of essential and non-essential amino acids (61.76%/38.24%). The results revealed significant differences (p < 0.05) in fatty acid profiles among the three dairy products for saturated short-chain, n-3, and n-6 fatty acids. Conversely, no significant difference (p < 0.05) was observed in monounsaturated fatty acids content. Kajmak showed the most favorable anti-atherogenic and anti-thrombogenic properties due to lower saturated and higher polyunsaturated fatty acid content. These results confirmed that buffalo milk could be successfully used in producing high-quality traditional dairy products with added value and beneficial characteristics from the aspect of a healthy diet. Furthermore, it could actively contribute to the promotion of sustainable production of buffaloes and strengthen the agricultural production of rural areas and their heritage.

Download Full-text

Meat Quality, Fatty Acid Content and NMR Metabolic Profile of Dorper Sheep Supplemented with Bypass Fats

Foods ◽

10.3390/foods10051133 ◽

2021 ◽

Vol 10 (5) ◽

pp. 1133

Author(s):

Atique Ahmed Behan ◽

Muhammad Tayyab Akhtar ◽

Teck Chwen Loh ◽

Sharida Fakurazi ◽

Ubedullah Kaka ◽

...

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Meat Quality ◽

Unsaturated Fatty Acids ◽

Saturated Fatty Acids ◽

Fatty Acid Content ◽

Basal Diet ◽

Negative Influence ◽

Significant Difference ◽

Dorper Sheep

The supplementation of rumen bypass fat (RBF) has remained one of the preferred approaches used to decrease undesirable saturated fatty acids (FA) and increase beneficial unsaturated FA in the meat. This study was planned to evaluate the influences of rumen bypass fats on meat quality, fatty acid and metabolic profiles in male Dorper sheep (n = 36) with 24.66 ± 0.76 kg (mean ± standard error) initial body weight. Treatment comprised a basal diet (30:70 rice straw to concentrate) with no added RBF as a control (CON), basal diet with prilled fat (PF), basal diet with prilled fat plus lecithin (PFL) and basal diet with calcium soap of palm fatty acids (CaS). The findings revealed that cooking loss, drip loss and shear force in longissimus dorsi (LD) muscle were not affected by RBF supplementation, while meat pH was significantly higher in the CaS on aging day 1. However, the diet supplemented with prilled fat and lecithin modified the meat’s fatty acid profile significantly by increasing unsaturated fatty acids and decreasing saturated fats. The relative quantification of the major differentiating metabolites found in LD muscle of sheep showed that total cholesterol, esterified cholesterol, choline, glycerophosphocholine and glycerophospholipids were significantly lower in CaS and PFL diets, while glycerol and sphingomyelin were significantly higher in CaS and PFL diets. Most of the metabolites in the liver did not show any significant difference. Based on our results, the supplementation of protected fats did not have a negative influence on meat quality and the meat from Dorper sheep fed prilled fat with lecithin contained more healthy fatty acids compared to other diets.

Download Full-text

Nutritional Analysis and Quality Evaluation in Muscle of Crucifix Crab Charybdis feriatus From Three Wild Populations

Journal of Food Research ◽

10.5539/jfr.v3n2p27 ◽

2014 ◽

Vol 3 (2) ◽

pp. 27 ◽

Cited By ~ 2

Author(s):

Jian-xue Lu ◽

Yang-yang Gong ◽

Yan-qing Huang ◽

Hong-yu Ma ◽

Xiong Zou ◽

...

Keyword(s):

Amino Acids ◽

Fatty Acid ◽

Unsaturated Fatty Acids ◽

Fatty Acid Content ◽

Total Content ◽

Essential Amino Acids ◽

Maturation Stage ◽

Wild Populations ◽

Nutritional Analysis ◽

Significant Difference

Three wild populations of crucifix crab Charybdis feriatus were sampled and muscle was analyzed for proximate composition, amino acid and fatty acid composition, aimed to quantify and compare the nutritional quality from three different locations in China. Results showed that crude protein content in muscle of female crucifix crab C. feriatus from Zhoushan (ZS) and Xiapu (XP) (84.84%-88.35%) were significantly higher than that of crucifix crab C. feriatus from Qionghai (QH) (74.33%), while there was no significant difference in terms of crude fat content (3.82%-4.07%). The highest content of ash was found in muscle of crucifix crab C. feriatus from QH (5.36%). The muscle of crucifix crab C. feriatus from ZS and XP had significantly higher contents of total amino acids, essential amino acids, non-essential amino acids and delicious amino acids than those of QH group (P < 0.05). The total saturated and unsaturated fatty acid content in the muscle of crucifix crab C. feriatus from three wild populations showed significant difference (P < 0.05). The ZS group had highest content of poly-unsaturated fatty acids (33.64%) and total content of EPA and DHA (22.85%) as well, followed by XP and QH group (P < 0.05). Overall, the differences in chemical composition in muscle of C. feriatus from different locations could be attributed to environmental variables and maturation stage.

Download Full-text

Sludge char-to-fuel approaches based on the hydrothermal fueling IV: fermentation

Water Science & Technology ◽

10.2166/wst.2021.281 ◽

2021 ◽

Author(s):

Jinyi Qin ◽

Rui Zhang ◽

Ruiwen Yang ◽

Jiao Fang ◽

Yu Zhang ◽

...

Keyword(s):

Amino Acids ◽

Fatty Acids ◽

Organic Matter ◽

Fatty Acid ◽

Sewage Sludge ◽

Acid Content ◽

Saturated Fatty Acids ◽

Fatty Acid Content ◽

Heating Value ◽

Cyclic Amino Acids

Abstract Sewage sludge was subjected to hydrothermal fueling (HTF) (330 °C for 40 min), obtaining hydrochar at 13.5 MJ kg−1. The higher heating value (HHV) of the raw sludge was related to its fatty acid content. The results showed that although the higher heating value (HHV) of the raw sludge was related to its fatty acid content, with the intensification of HTF, the increase in aliphatic/cyclic amino acids determined the production of HHV in the hydrochar. In order to increase the content of fatty acids and amino acids, the sludge was fermented. However, the Bacteroidetes consumed the organic matter too early, which was detrimental to the production of HHV. Therefore, appropriate sludge fermentation is recommended to restrict excessive Bacteroidetes proliferation, decompose lipids to saturated fatty acids, and convert proteins to aliphatic/cyclic amino acids to increase the efficiency of converting sludge to fuel.

Download Full-text

Quality of Meat from Female Fallow Deer (Dama Dama) and Roe Deer (Capreolus Capreolus) Hunted in Serbia

Annals of Animal Science ◽

10.2478/aoas-2019-0064 ◽

2020 ◽

Vol 20 (1) ◽

pp. 245-262

Author(s):

Snežana Ivanović ◽

Boris Pisinov ◽

Marija Pavlović ◽

Ivan Pavlović

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Chemical Composition ◽

Volatile Compounds ◽

Acid Content ◽

Roe Deer ◽

Saturated Fatty Acids ◽

Fatty Acid Content ◽

Fallow Deer ◽

Significant Difference

AbstractDeer meat is a high quality and valuable food for human consumption. It has high nutritive value because of its high protein and heme iron content, and low levels of fats and saturated fatty acids. The aim of this study was to examine the quality parameters of meat from fallow deer and roe deer that were hunted in Serbia. Parameters studied were live weight, carcass weight, chemical composition of meat, color, fatty acid content of meat, volatile compounds, and sensory characteristics. The results obtained show no significant difference in the chemical composition of these two species of deer meat, but there were differences regarding fatty acid content, volatile compounds, color and sensory properties of meat. The ratios of polyunsaturated to saturated fatty acids in the deer meat ranged from 0.387 to 0.556. The results suggest that deer species has a significant impact on the fatty acid profile and content of volatile compounds of deer meat.

Download Full-text

Morinda citrifolia: Amino Acid and Lipid Content of the Noni Fruit at Various Stages of Maturity

Journal of Scientific Research ◽

10.3329/jsr.v4i2.8130 ◽

2012 ◽

Vol 4 (2) ◽

pp. 467 ◽

Cited By ~ 1

Author(s):

K. D. Golden ◽

C. Lindsay

Keyword(s):

Amino Acids ◽

Fatty Acids ◽

Fatty Acid ◽

Amino Acid ◽

Acid Content ◽

Fatty Acid Content ◽

Essential Amino Acids ◽

Morinda Citrifolia ◽

Ripe Stage ◽

Green Stage

High performance liquid chromatography (HPLC) was used to determine the amino acid and fatty acid content of Morinda citrifolia (Noni). The amino acid content both free and total was determined at the mature green, mature ripe and fermented stage of the fruit. Most of the essential amino acids were detected as free amino acids (unhydrolysed sample) at the various stages of ripening, with threonine showing the highest value (3.95±0.81 mg/100g) at the fermented stage and histidine showing the lowest value (0.05±0.02 mg/100g) at the mature green stage. The essential amino acids with the exception of tryptophan (destroyed by acid hydrolysis) were also detected as total amino acids (hydrolyzed sample) albeit at much higher concentrations. Leucine showed the highest value (94.21±18.85 mg/100g) at the mature ripe stage, whereas methionine the lowest value (2.80±1.02 mg/100g) at the mature green stage. The fatty acid content of the Noni fruit was also determined at the green, ripe and fermented stages of ripening; with the short chain caprylic acid (80.69±3.09 mg/100g) showing the highest value at the ripe stage. Significantly the essential fatty acids linolenic (8.60±0.21 mg/100g) and linoleic (50.57±0.72 mg/100g) were highest at the green stage. Also present in significant quantities were palmitic acid (44.27±0.35 mg/100g) and stearic acid (4.78±0.45 mg/100g) at the green stage. The amount of fatty acids decreased significantly at the fermented stage. Of the fatty acids detected at the fermented stage 79% of them were below 1.00 mg/100g fresh weight of the fruit. Keywords: Noni fruit; Amino acids; Fatty acids; HPLC. © 2012 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. doi: http://dx.doi.org/10.3329/jsr.v4i2.8130 J. Sci. Res. 4 (2), 467-476 (2012)

Download Full-text

TOTAL LIPID, FREE STEROL, FREE FATTY ACID, AND TRIACYLGLYCEROL FATTY ACID CONTENT OF TOBACCO MOSAIC VIRUS-INFECTED TOBACCO

Canadian Journal of Plant Science ◽

10.4141/cjps82-111 ◽

1982 ◽

Vol 62 (3) ◽

pp. 771-776 ◽

Cited By ~ 5

Author(s):

L. E. TREVATHAN ◽

S. A. TOLIN ◽

L. D. MOORE ◽

D. M. ORCUTT

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Tobacco Mosaic Virus ◽

Mosaic Virus ◽

Total Lipid ◽

Free Sterol ◽

Fatty Acid Content ◽

Free Sterols ◽

Significant Difference ◽

Infected Tobacco

North Carolina 88 tobacco, a systemic host of tobacco mosaic virus (TMV), was inoculated at the third leaf stage with purified TMV. Inoculated, asymptomatic, and systemically infected, symptomatic leaves were harvested 24 days after inoculation and analyzed for total lipids, free sterols, free fatty acids and triacylglycerol fatty acids. Infection resulted in significant quantitative increases in free sterol and decreases in both free and triacylglycerol fatty acid concentrations, but no changes in total lipid concentrations. Changes in all lipid components assayed occurred in TMV-inoculated leaves as well as in systemically infected leaves, with the only significant difference between them being a lower concentration of the triacylglycerol fatty acid, linolenic acid, in systemically infected leaves.

Download Full-text

Description of amino acid and fatty acid content during initial development of Lophiosilurus alexandri (Siluriformes: Pseudopimelodidae), a carnivorous freshwater catfish

Neotropical Ichthyology ◽

10.1590/1982-0224-20180014 ◽

2018 ◽

Vol 16 (2) ◽

Cited By ~ 2

Author(s):

Deliane Cristina Costa ◽

Rodrigo Takata ◽

Walisson de Souza e Silva ◽

Martin Bessonart ◽

Juan Luis Gadea ◽

...

Keyword(s):

Amino Acids ◽

Fatty Acids ◽

Fatty Acid ◽

Amino Acid ◽

Acid Content ◽

Fatty Acid Content ◽

Monounsaturated Fatty Acids ◽

Initial Development ◽

Polyunsaturated Fatty Acid Content ◽

Saturated Fatty Acid Content

ABSTRACT Samples of eggs, newly hatched larvae (NHL), and larvae at the end of the lecithotrophic period (eight days after hatching) (LPL) of Lophiosilurus alexandri were collected to determine the amino acid and fatty acid profiles. Crude protein did not change throughout initial development and the concentration of lipids was highest in NHL. The content of the indispensable amino acids (IAA) isoleucine, leucine, and valine decreased in LPL, while in eggs and NHL they remained high and similar in value. The dispensable amino acids (DAA), such as aspartic acid, tyrosine, and glycine, increased in LPL, while alanine decreased. The percentage of neutral lipids increased in LPL. The saturated fatty acid content decreased during ontogeny, while monounsaturated fatty acids decreased only in LPL. The polyunsaturated fatty acid content was highest in LP. Polar fatty acids were found in higher percentages in eggs and NHL, but lower in LPL. Saturated fatty acid content decreased during ontogenetic development, while that of monounsaturated fatty acids decreased only in LPL. Polyunsaturated fatty acid content was highest in LPL. Protein content was maintained during ontogenetic development, while amino acid classes experienced changes. Lophiosilurus alexandri preferentially uses saturated and monosaturated fatty acids as an energy source during its early development.

Download Full-text

Ripening changes in Kopanisti cheese

Journal of Dairy Research ◽

10.1017/s0022029900026893 ◽

1990 ◽

Vol 57 (2) ◽

pp. 271-279 ◽

Cited By ~ 7

Author(s):

Stelios E. Kaminarides ◽

Emmanuel M. Anifantakis ◽

Efstathios Alichanidis

Keyword(s):

Amino Acids ◽

Fatty Acids ◽

Fatty Acid ◽

Free Fatty Acid ◽

Free Amino ◽

Fatty Acid Content ◽

Aminobutyric Acid ◽

Total N ◽

Soft Cheese ◽

Long Chain

SummaryChanges in a Greek traditional soft cheese, Kopanisti, were followed during ripening. Mature cheese contained > 52% moisture, 22·7% fat and 19·3% protein. The as-casein content was hydrolysed faster than the β-casein so that in mature cheese only 23% and 35% respectively of these proteins remained intact. Soluble N, TCA-soluble N, amino acid N and NH3 were present to the extent of 28·9, 24·6, 14·3 and 6·6% of total N respectively. Leucine, γ-aminobutyric acid, valine and alanine were the dominant free amino acids in the mature cheese. Lipolysis was intense and the total free fatty acid content of ripe cheese was ∽ 50 g/kg cheese. The rate of liberation of short-chain (C4–C8) fatty acids was higher than that of the long-chain. The characteristic rich flavour and peppery taste appeared after 16 d ripening and the best overall cheese quality was produced after 32 d maturation.

Download Full-text

STUDY ON THE VARIATIONS OF THE FATTY ACIDS AND AMINO ACIDS OF GREEN PEEL AVOCADO IN SON LA PROVINCE AT DIFFERENT HARVESTING MATURITY

Journal of Science Natural Science ◽

10.18173/2354-1059.2021-0053 ◽

2021 ◽

Vol 66 (3) ◽

pp. 124-130

Author(s):

Loi Nguyen Van

Keyword(s):

Amino Acids ◽

Fatty Acids ◽

Fatty Acid ◽

Amino Acid ◽

Acid Content ◽

Developmental Stages ◽

Fatty Acid Content ◽

Amino Acid Content ◽

Hplc Method ◽

Dried Fruit

Green peel avocado is a fruit with high nutritional value, rich in lipids, proteins, sugars, vitamins, and minerals. The aim of this study was to determine the change in fatty acid and amino acid content of green peel avocados harvested at 200, 210 and 220 days in order to select the appropriate harvest time, serving for efficient preservation and processing. The results of the study showed that 9 fatty acids were identified at the time of harvesting at 200 days, 10 fatty acids at the time of harvesting at 210, and 220 days of green peel avocado. Compared with two harvest times as 210, and 220 days since fruiting, the fatty acid content of green peel avocado did not differ so much. By using the HPLC method, 13 amino acids, 14 amino acids, and 15 amino acids were identified at 200, 210, and 220 days after fruiting, respectively. Glycine, alanine, lysine, and phenylalanine were intended to increase in the developmental stages of green peel avocado, whereas the other amino acids did not change much. Therefore, based on the change in fatty acid and amino acid content, green peel avocado farmers in Son La province should choose the time of harvest from 210 to 220 days from fruiting to be suitable for preservation and processing. If harvesting green peels avocados at more than 220 days after fruiting, it will make the dried fruit and spongy, and affect flowering, fruit set, and yield in the next fruit crop.

Download Full-text

Effect of Nitrogen Fertilisation and Inoculation with Bradyrhizobium japonicum on the Fatty Acid Profile of Soybean (Glycine max (L.) Merrill) Seeds

Agronomy ◽

10.3390/agronomy11050941 ◽

2021 ◽

Vol 11 (5) ◽

pp. 941

Author(s):

Ewa Szpunar-Krok ◽

Anna Wondołowska-Grabowska ◽

Dorota Bobrecka-Jamro ◽

Marta Jańczak-Pieniążek ◽

Andrzej Kotecki ◽

...

Keyword(s):

Fatty Acids ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Acid Composition ◽

Bradyrhizobium Japonicum ◽

Field Experiments ◽

Fatty Acid Content ◽

Soybean Seeds ◽

Oilseed Crop ◽

Soybean Cultivars

Soybean is a valuable protein and oilseed crop ranked among the most significant of the major crops. Field experiments were carried out in 2016–2019 in South-East Poland. The influence of soybean cultivars (Aldana, Annushka), nitrogen fertilizer (0, 30, 60 kg∙ha−1 N) and inoculation with B. japonicum (control, HiStick® Soy, Nitragina) on the content of fatty acids (FA) in soybean seeds was investigated in a three-factorial experiment. This study confirms the genetic determinants of fatty acid composition in soybean seeds and their differential accumulation levels for C16:0, C16:1, C18:1n9, C18:2, C18:3, and C20:0 as well saturated (SFA), monounsaturated (MUFA), and polyunsaturated (PUFA) fatty acids. Increasing the rate from 30 to 60 kg ha−1 N did not produce the expected changes, suggesting the use of only a “starter” rate of 30 kg ha−1 N. Inoculation of soybean seeds with a strain of Bradyrhizobium japonicum (HiStick® Soy, BASF, Littlehampton, UK and Nitragina, Institute of Soil Science and Plant Cultivation–State Research Institute, Puławy, Poland) is recommended as it will cause a decrease in SFA and C16:0 acid levels. This is considered nutritionally beneficial as its contribution to total fatty acids determines the hypercholesterolemic index, and it is the third most accumulated fatty acid in soybean seeds. The interaction of cultivars and inoculation formulation on fatty acid content of soybean seeds was demonstrated. An increase in the value of C16:0 content resulted in a decrease in the accumulation of C18:1, C18:2, and C18:3 acids. The content of each decreased by almost one unit for every 1% increase in C16:0 content. The dominant effect of weather conditions on the FA profile and C18:2n6/C18:3n3 ratio was demonstrated. This suggests a need for further evaluation of the genetic progress of soybean cultivars with respect to fatty acid composition and content under varying habitat conditions.

Download Full-text