scholarly journals Assessment of the effect of long-term seed storage on the viability of Matricaria chamomilla seeds after cryopreservation

2021 ◽  
Vol 103 (3) ◽  
pp. 95-101
Author(s):  
Alibek Ramasanov ◽  
◽  
Larissa Babeshina ◽  
Keyword(s):  
Seed Germination ◽  
Liquid Nitrogen ◽  
Room Temperature ◽  
Germination Rate ◽  
Seed Storage ◽  
Water Bath ◽  
Moscow Region ◽  
Matricaria Chamomilla ◽  
Test Species ◽  
Viable Seeds

The article presents the study of influences of cryopreservation on seed germination of Matricaria chamomilla. Four varieties «Moscow Region», «Karagandinskaya», «Aibolit» and «Old Lekar» are used in the work. The seeds of the test species are cryopreserved in liquid nitrogen, followed by defrosting at room temperature and rapid defrosting in a water bath at temperature +40 Ԩ. After cryopreservation the seeds are planted in Petri dishes and placed in a climate chamber for determination laboratory germination. It is noted that the best results are obtained in the defrosting variant at the room temperature. So the maximum germination rate for variety «Karagandinskaya» was 84.0 %, energy of germination — 83.0 %; for «Aibolit» variety was 82.0 % and 81.0 %, respectively; «Moscow Region» variety had a minimum germination rate — 13.0 % and energy of germination 10.0 %. While for the «Old Lekar» variety, the optimal thawing mode was rapid thawing in a water bath at the temperature +40Ԩ; seed germination was 63.0 %, energy of germination — 62.0 %. Thus, it is necessary for receiving more viable seeds of varieties of Matricaria chamomilla after freezing in liquid nitrogen to apply the slow mode of thawing at the room temperature +24 Ԩ.

scholarly journals Study of the Cryopreservation Conditions for seed Materials of Chartolepis intermedia Boiss.

2021 ◽  
Vol 38 ◽  
pp. 00046
Author(s):  
Margarita Ishmuratova ◽  
Saltanat Tleukenova ◽  
Alibek Ramasanov ◽  
Elena Gavrilkova ◽  
Dmitrii Ageev
Keyword(s):  
Seed Germination ◽  
Liquid Nitrogen ◽  
Room Temperature ◽  
Germination Rate ◽  
Plastic Container ◽  
In The Wild ◽  
First Time ◽  
Karaganda Region

For the first time, the depending of germination rate and energy of germination of Chartolepis intermedia seeds, collected in the wild of Karaganda region, from morphology of seeds and conditions of cryopreservation is investigated. The maximum results for viability are fixed for dark-colored average or large seeds. The best results are determined for variant of cryopreservation in plastic container with future defrosting at the room temperature. For the increasing parameters of seed germination we recommended to freeze Chartolepis intermedia seeds with using cryoprotector sucrose in concentration 20%. As the results of the study, we developed algorithm of cryopreservation of Chartolepis intermedia seeds in liquid nitrogen.

scholarly journals Germination and seed conservation of a pioneer species, Tecoma stans (Bignoniaceae), from tropical dry forest of Colombia

2018 ◽  
Vol 66 (2) ◽  
pp. 918
Author(s):  
Jhon Alexander Vargas-Figueroa ◽  
Alba Marina Torres-González
Keyword(s):  
Seed Germination ◽  
White Light ◽  
Germination Rate ◽  
Red Light ◽  
Tropical Dry Forest ◽  
Seed Storage ◽  
Dry Forest ◽  
Storage Behavior ◽  
Mean Germination Time ◽  
Seed Storage Behavior

Seed germination and seed longevity under different environmental conditions are fundamental to understand the ecological dynamics of a species, since they are decisive for its success within the ecosystem. Taking this into account, seed germination and seed storage behavior of a pioneer species of tropical dry forest (Tecoma stans) were studied in the laboratory, to establish the effect of different environmental conditions on a local tree population. Two seed lots collected in July 2011, from Cali (Colombia), were evaluated under three alternating temperatures (20/30, 20/25, 25/30 ºC; 16/8 h) and four light qualities (12-hour white light photoperiod, darkness, and 15 minutes of red light -R and far red light -FR). Final germination was recorded for all treatments; for white light treatment the daily germination was recorded to calculate mean germination rate, mean germination time, and two synchronization indices. To assess the effect of light quality on physiological variables, a destructive germination test was carried out. For this test, another seed lot was evaluated under the same light conditions using an alternating temperature of 20/30 °C - 16/8 h, recording germination during six days for every treatment. In addition, seeds were stored at two different moisture contents (7.7, 4.1 %) and three storage temperatures (20, 5, -20 ºC), during two time periods (one and three months); a seed germination test was conducted for each treatment. Four replicates of 35 seeds per treatment were used for all experiments. Germination was high (GP > 90 %) with all alternating temperatures under white light, whereas under R, FR, and darkness germination was evenly successful at low temperatures, but at higher temperature, half of the seeds entered into secondary dormancy (GP= 45-65 %). However, mean germination rate and synchronization under R and FR decreased significantly in comparison to white light treatment and, consequently, mean germination time increased. Seed storage behavior of this species is orthodox due to the high germination (GP > 90 %) obtained under all treatments. In conclusion, T. stans seeds have a negative germination response at high incubation temperatures in the absence of white light, entering into a secondary dormancy. In contrast, an environment with a lower temperature and without white light delays the germination, but at the end seeds are able to reach the same germination values. This seed dependence on incident light in limiting conditions suggests a physiological mechanism on the seed tissues of this species, probably mediated by phytochromes. Finally, the orthodox seed storage behavior of T. stans is a reason to include this species in ex situ seed conservation programs for restoration and recovery of the tropical dry forest; however, long-term studies should be conducted in order to evaluate the maintenance of this characteristic throughout longer periods of time. Rev. Biol. Trop. 66(2): 918-936. Epub 2018 June 01. 

scholarly journals Seed Germination and Storage of the Endangered Species Manglietia crassipes Y. W. Law (Magnoliaceae)

Horticulturae ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 42
Author(s):  
Ai-Hua Wang ◽  
Xiao-Ling Yu ◽  
Yan-Yan Liu ◽  
Shu-Gang Chen ◽  
Fa-Guo Wang
Keyword(s):  
Seed Germination ◽  
Endangered Species ◽  
Seed Dormancy ◽  
Germination Rate ◽  
Seed Storage ◽  
Storage Conditions ◽  
Seed Vigor ◽  
Water Control ◽  
Critically Endangered Species ◽  
Germination Characteristics

Manglietia crassipes, a critically endangered species narrowly distributed on Mount Dayao in Guangxi, China, is also a species of ornamental interest whose variability has not been explored. Key factors leading to its endangerment have also not been studied. Here, two experiments were conducted to test the effects of different plant growth regulators and different storage conditions on germination characteristics of M. crassipes seeds. Fruit morphology was measured, and germination characteristics of fresh mature seeds were tested in order to assess natural seed vigor. Seeds were soaked in distilled water (control), or gibberellic acid (GA3), 6-benzylaminopurine (6-BA) or indoleacetic acid (IAA) solutions of different concentrations, for 48 h to determine their effects on seed germination. In addition, the effects of different seed storage conditions (constant 4 °C, −7 °C, −20 °C, or 25 °C for 100 days, wet stratification at 4 °C for 100 days) on seed germination were investigated. Results showed that the abortive rate of the mature fruits was high (28.9%) and fresh natural seeds had a low germination rate (G) and germination index (GI). Seed germination was inhibited with 6-BA at all concentrations, but only at less than 100 m·L−1 IAA; otherwise, IAA slightly promoted seed germination. GA3 broke seed dormancy and significantly accelerated seed germination by improving G, GI and initial germination time (IGT), especially over 2500 mg·L−1. The viability of seeds declined no matter how they were stored. However, 4 °C wet stratification storage was preferable for seed vigor and germination power. Our results suggest that the high abortive rate of fruits, low germination of the natural seed, seed dormancy and its intolerance to storage, contributed to the endangerment of M. crassipes. GA3 can break M. crassipes seed dormancy, which can be a benefit for future ornamental breeding and further protection or conservation.

EFFECTS OF STORAGE CONDITIONS AND PRESENCE OF FRUITING BRACTS ON THE GERMINATION OF ATRIPLEX HALIMUS AND SALSOLA VERMICULATA

1997 ◽  
Vol 33 (2) ◽  
pp. 149-155 ◽  
Author(s):  
A. E. OSMAN ◽  
F. GHASSALI
Keyword(s):  
Seed Germination ◽  
Cold Storage ◽  
North Africa ◽  
Room Temperature ◽  
Storage Temperature ◽  
Seed Storage ◽  
Storage Conditions ◽  
Shrub Species ◽  
Atriplex Halimus ◽  
Different Temperatures

Two shrub species, Atriplex halimus L. and Salsola vermiculata L., are considered useful for rehabilitation of degraded rangelands in west Asia and north Africa. They can be established from direct seeding and are capable of self-sowing. In this study, seed storage at different temperatures and the influence of fruiting bracts on seed germination were examined for the two species during two seasons. Fruits (utricles) were stored at 20–22°C (room temperature), 0°C or −22°C. Germination tests were carried out after 33, 56, 90, 152, 272 and 397 d in storage in the first season and after 44, 76, 104, 170, 288 and 412 d in the second season. Seeds were germinated in their fruiting bracts or after bract removal. Bract removal significantly improved seed germination of both shrubs regardless of storage temperature. For S. vermiculata the increase in germination was in the range of 1.3- to 14.7-fold compared with values for the intact fruit in Season 1 and 0.5 to 3.8 in Season 2. Similarly the ranges for A. halimus were 0.5- to 4.2-fold and 0.7- to 5.3-fold in the two seasons respectively. The effect of cold storage was greater on Salsola than on Atriplex. The reduction of the storage temperature from 21°C to 0°C and −22°C increased the longevity of S. vermiculata seeds by 2.8–46.6 times in Season 1 and by 2.9–2.6 times in Season 2. There was little or no effect on the longevity of A. halimus. A leachate prepared by soaking fruiting bracts from S. vermiculata significantly depressed germination (p < 0.01), the effect being greater on Salsola seeds (20% reduction) than on Atriplex seeds (8% reduction). A leachate from A. halimus produced a slight but non-significant reduction in germination.

scholarly journals Effect of storage conditions and sodium hypochlorite treatment on germination of Cucumis prophetarum (Cucurbitaceae) seeds from arid Arabian deserts

2021 ◽  
Author(s):  
Hatem Ahmed Shabana ◽  
Tamer Mahmoud ◽  
Sanjay Gairola ◽  
Asma Al Ketbi ◽  
Mariam Aljasmi ◽  
...  
Keyword(s):  
Seed Germination ◽  
United Arab Emirates ◽  
Sodium Hypochlorite ◽  
Room Temperature ◽  
Seedling Emergence ◽  
Seed Storage ◽  
Storage Conditions ◽  
Germination Percentage ◽  
Continuous Darkness ◽  
Temperature Requirements

Abstract Background This is the first study on seed germination of Cucumis prophetarum in the Arabian Peninsula. Light and temperature as the main environmental factors and seed storage conditions greatly affect the germination of many Cucurbitaceae species. C. prophetarum is the perennial prostrate with woody rootstocks that grow throughout the year in the arid Arabian deserts. We examined the effects of seed storage conditions and sterilization by sodium hypochlorite (NaOCL) on germination of C. prophetarum seeds. Matured, fresh and field stored seeds were collected in March of 2016 and 2017 from a population in the United Arab Emirates. Fresh and the stored seeds (field, freeze, and room temperature) were germinated at three temperature regimes (15/25, 20/30 and 25/35°C) in both continuous darkness and alternating 12 h light/12 h darkness. Results The fresh and freeze stored seeds had higher germination percentage than the field and room temperature stored seeds. High germination was recorded in both lower and moderate temperatures (15/25 and 20/30°C) in light conditions. Sterilization of the seeds by NaOCl had a negative effect on the speed and germination percentage under all storage conditions, and it changes light and temperature requirements of seeds for germination. Conclusions Germination of C. prophetarum is sensitive to incubation light and temperature as well as to the seed storage conditions. The germination ability of stored seeds indicates that seed storage behaviour of C. prophetarum is orthodox. Light and temperature requirements for seed germination reflects a suitable place, habitat, and time for seedling emergence of C. prophetarum. The effect of NaOCl treatment on the germination may be associated with concentration and exposure periods of seeds. Therefore, we suggest that when using NaOCl for seed sterilization or to stimulate the germination, caution must be taken for using its concentration and exposure time, especially for species with thin and soft seed coats like C. prophetarum. Further studies on seed germination ecology would help to understand better the adaptive strategy of the C. prophetarum in the arid desert environments.

scholarly journals Impact of storage conditions on seed germination and seedling growth of wild oat (Avena fatua L.) at different temperatures

2015 ◽  
Vol 30 (4) ◽  
pp. 243-248
Author(s):  
Marija Saric-Krsmanovic ◽  
Gajic Umiljendic ◽  
Ljiljana Santric ◽  
Ljiljana Radivojevic
Keyword(s):  
Seed Germination ◽  
Germination Rate ◽  
Seed Storage ◽  
Storage Conditions ◽  
Avena Fatua ◽  
Shoot Length ◽  
Wild Oat ◽  
Different Temperatures ◽  
C 30 ◽  
Germinated Seeds

The influence of seed storage conditions and different temperatures (5?C, 10?C, 15?C, 20?C, 25?C, 30?C and 26?C/21?C) during germination and seedling development on seed germination, shoot length and germination rate of wild oat (Avena fatua L.) was examined. Germinated seeds were counted daily over a period of ten days and shoot length was measured on the last day, while germination rates were calculated from those measurements. The results showed that seed storage under controlled conditions (T1: temperature 24?1?C, humidity 40-50%; T2: temperature 26?1?C, humidity 70-80% and T3: temperature 4?C) for periods of 3 (t1) and 12 (t2) months had a significant influence on germination of wild oat seeds. The percentage of germinated seeds under all examined temperatures was higher when they were stored for 12 months under controlled temperature and humidity. The results also showed that temperature had a significant effect on the percentage of germination and germination rate of A. fatua seeds. The highest total germination occurred at 15?C temperature (T1: t1 - 41.25%, t2 - 44.37%; T2: t1 - 28.13%, t2 - 34.37%; T3: t1 - 10.63%, t2 - 12.50%). Germination percentage under an alternating day /night photoperiod at 26?C/21?C temperature was higher in all treatment variants (T1: t1 - 8.13%, t2 - 10.00%; T2: t1 - 11.87%, t2 - 13.13%; T3: t1 - 2.42%, t2 - 2.70%) than germination in the dark at 25?C, 30?C and 5?C.

scholarly journals Effects of ultra-dry storage on seed germination and seedling growth of Handeliondendron bodinieri

Silva Fennica ◽  
2021 ◽  
Vol 55 (3) ◽  
Author(s):  
Chuan Xie ◽  
Tianfeng Liu ◽  
Song Guo ◽  
Jian Peng ◽  
Zailiu Li
Keyword(s):  
Polyethylene Glycol ◽  
Seed Germination ◽  
Moisture Content ◽  
Seedling Growth ◽  
Germination Rate ◽  
Soluble Sugar ◽  
Seed Storage ◽  
Seed Vigor ◽  
Protein Accumulation ◽  
Dry Storage

(H. Lév.) Rehder is a rare, endangered, and therefore, protected tree species native to China. However, there are serious limitations to the effective protection of the species, including a low seed germination-rate and difficult storage due to a high seed oil-content. Here, we evaluated the feasibility of ultra-dry seed storage and its effects on seedling growth. We used the silica gel method to prepare ultra-dry seeds with different moisture contents to find an optimal moisture content range (2.54%–4.77%). Ultra-dry treatment improved storability of . Furthermore, seeds with a moisture content of 4.77% stored at room temperature, and seeds with a moisture content of 3.97% stored at 4 °C yielded the best results. Priming with an appropriate concentration of polyethylene glycol had a certain repairing effect on ultra-dry stored seeds and improved seed vigor, with a two-day priming treatment with 20% polyethylene glycol having the best effect. Finally, compared with sand storage at 4 °C, ultra-dry storage promoted seedling growth and root development; furthermore, it alleviated storage damage to seeds, promoted soluble sugar and soluble protein accumulation, and increased seedling nitrogen, phosphorus, and potassium uptake. Therefore, ultra-dry storage can be effectively used to preserve seeds. Specifically, low-temperature storage of ultra-dry seeds with a moisture content of 3.97% enhanced seed vigor, and seedling growth and development.Handeliodendron bodinieriH. bodinieri seedsH. bodinieriH. bodinieriH. bodinieri

scholarly journals Alpine Pioneer Plants in Soil Bioengineering for Slope Stabilization and Restoration: Results of a Preliminary Analysis of Seed Germination and Future Perspectives

2020 ◽  
Vol 12 (17) ◽  
pp. 7190
Author(s):  
Luca Giupponi ◽  
Valeria Leoni
Keyword(s):  
Calcium Carbonate ◽  
Seed Germination ◽  
Room Temperature ◽  
Restoration Ecology ◽  
Germination Rate ◽  
Ex Situ ◽  
Pioneer Species ◽  
Soil Bioengineering ◽  
Pioneer Plants ◽  
Dryas Octopetala

Stabilization of slopes subject to landslide by measures with low impact, such as those of bioengineering, is a topic of interest. The use of scarcely studied alpine pioneer plants could contribute to innovation in soil bioengineering and restoration ecology but to use them, knowledge of the ex situ germinability of their seeds is fundamental. This research analysed the germinability of seeds of nine alpine pioneer species (Papaver aurantiacum, Rumex scutatus, Tofieldia calyculata, Pulsatilla alpina, Silene glareosa, Adenostyles alpina, Dryas octopetala, Laserpitium peucedanoides and Laserpitium krapfii) treated with water, gibberellic acid (GA3) and/or calcium carbonate at room temperature. The seeds had different responses to the treatments: Laserpitium peucedanoides, L. krapfii and Silene glareosa showed difficulty in germinating (germination < 2.5%), while Dryas octopetala had good germination (39–61%) regardless of treatment. GA3 significantly increased the seed germination rate of Papaver aurantiacum, Pulsatilla alpina, Rumex scutatus and Tofieldia calyculata, while the addition of calcium carbonate made the seeds of Rumex scutatus and Tofieldia calyculata germinate more quickly. Results are discussed focusing on the perspectives of using alpine pioneer species in future soil bioengineering work for slopes stabilization and restoration, and on the actions that stakeholders should take to make this happen.

Durum wheat (Triticum durum Desf.) seed storage under controlled conditions

2020 ◽  
pp. 105-115
Author(s):  
O.A. Zadorozhna ◽  
T. P. Shyianova ◽  
M.Yu. Skorokhodov
Keyword(s):  
Seed Germination ◽  
Moisture Content ◽  
Durum Wheat ◽  
Germination Rate ◽  
Seed Storage ◽  
Seed Longevity ◽  
Forest Steppe ◽  
Controlled Conditions ◽  
Seed Moisture ◽  
Wheat Seeds

Aim. The aim of this work was to determine the longevity of durum wheat seeds according to the results of seed germination monitoring after seed storage for up to 19 years under controlled conditions in the National Plant Gene Bank of Ukraine. Results and Discussion. 36 accessions of spring durum wheat were investigated. These accessions belonged to var. hordeiforme, var. leucurum, var. melanopus, var. alexandrinum, var. apulicum, var. australe from seven countries; seven samples of durum winter wheat belonged to var. hordeiforme, var. leucurum were from Ukraine. Accessions were received by the Ukrainian genebank from seven countries: Ukraine, Russia, Mexico, France, Portugal, Kazakhstan and Tunisia. Seed accessions for storage were grown mainly in the eastern forest-steppe of Ukraine, stored in the National depositary in this region at unregulated temperature and at 4°C with seed moisture content of 5.5-8.0%. The mode of seed drying, which took place at temperature not higher than 25°C is discussed. The obtained results indicate high seed longevity of durum wheat under these conditions with initial seed germination rate more than 90% even in a storage facility at unregulated temperature. There were no differences in seed longevity between varieties of durum wheat under the studied storage conditions. Conclusions. Seeds of durum wheat remain unchanged for at least 10 years with a high initial germination of seeds, storage in sealed containers with moisture content 5.5-8.0% even at unregulated temperature of the eastern forest-steppe of Ukraine. Durum wheat seeds should be storage at temperature 4°C to increase seed longevity at seed moisture 5.5-8.0%.

scholarly journals The effect of cryopreservation on germination of dandelion seeds

2008 ◽  
Vol 43 (No. 2) ◽  
pp. 63-67 ◽  
Author(s):  
Z. Martinková ◽  
A. Honěk
Keyword(s):  
Seed Germination ◽  
Germination Rate ◽  
Seed Storage ◽  
Taraxacum Officinale ◽  
Germination Percentage ◽  
Systematic Change ◽  
Long Day ◽  
Germination Experiments ◽  
Germinating Seeds ◽  
Seed Cryopreservation

Germination experiments frequently use seeds that had been stored frozen. We investigated whether short, 5 day freezing changes percentage and rate of germination of seeds of dandelion (<i>Taraxacum officinale</i> agg.). Seeds (<i>i</i>) collected at dispersal, (<i>ii</i>) dried at +50°C for 5 days, (<i>iii</i>) frozen at –20°C for 5 days, (<i>iv</i>) dried for 5 days and subsequently frozen for 5 days, and (<i>v</i>) frozen and subsequently dried, were then germinated at +10°C and a long-day photoperiod. None of the temperature pre-treatments affected the proportion of germinating seeds. By contrast, the time to germinate 50% of the seed (germination time) was shortened slightly (0.7 days) but significantly following the freezing treatment, regardless of whether it was applied without, before or after drying of the seed. Cryopreservation is therefore a convenient method of seed storage for comparative studies of seed germination because it causes no change in germination percentage and only a small and systematic change in germination rate.

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