scholarly journals Characterization of a Novel SARS-CoV-2 Genetic Variant with Distinct Spike Protein Mutations

Viruses ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 1029
Author(s):  
Anna Gladkikh ◽  
Anna Dolgova ◽  
Vladimir Dedkov ◽  
Valeriya Sbarzaglia ◽  
Olga Kanaeva ◽  
...  
Keyword(s):  
Mortality Rate ◽  
South African ◽  
Genetic Variants ◽  
Genetic Variant ◽  
Biological Properties ◽  
Spike Protein ◽  
High Incidence ◽  
The World ◽  
Protein Mutations

The COVID-19 pandemic, which began in Wuhan (Hubei, China), has been ongoing for about a year and a half. An unprecedented number of people around the world have been infected with SARS-CoV-2, the etiological agent of COVID-19. Despite the fact that the mortality rate for COVID-19 is relatively low, the total number of deaths has currently already reached more than three million and continues to increase due to high incidence. Since the beginning of the pandemic, a large number of sequences have been obtained and many genetic variants have been identified. Some of them bear significant mutations that affect biological properties of the virus. These genetic variants, currently Variants of Concern (VoC), include the so-called United Kingdom variant (20I/501Y), the Brazilian variant (20J/501Y.V3), and the South African variant (20H/501Y.V2). We describe here a novel SARS-CoV-2 variant with distinct spike protein mutations, first obtained at the end of January 2021 in northwest Russia. Therefore, it is necessary to pay attention to the dynamics of its spread among patients with COVID-19, as well as to study in detail its biological properties

scholarly journals Molecular beacons allow specific RT-LAMP detection of B.1.1.7 variant SARS-CoV-2

2021 ◽  
Author(s):  
Scott Sherrill-Mix ◽  
Gregory D. Van Duyne ◽  
Frederic D. Bushman
Keyword(s):  
Genetic Variants ◽  
Molecular Beacons ◽  
Spike Protein ◽  
Detection Methods ◽  
Gene Encoding ◽  
Detection And Identification ◽  
Rapid Spread ◽  
The World ◽  
Primer Sets ◽  
Current Detection

AbstractOver the course of the COVID-19 pandemic, several SARS-CoV-2 genetic variants of concern have appeared and spread throughout the world. Detection and identification of these variants is important to understanding and controlling their rapid spread. Current detection methods for a particularly concerning variant, B.1.1.7, require expensive qPCR machines and depend on the absence of a signal rather than a positive indicator of variant presence. Here we report an assay using a pair of molecular beacons paired with reverse transcription loop mediated amplification to allow isothermal amplification from saliva to specifically detect B.1.1.7 and other variants which contain a characteristic deletion in the gene encoding the viral spike protein. This assay is specific, affordable and allows multiplexing with other SARS-CoV-2 LAMP primer sets.

scholarly journals Further Characterization of an Interleukin-2-1Ike Cytokine Produced byXenopus LaevisT Lymphocytes

1993 ◽  
Vol 3 (3) ◽  
pp. 231-238 ◽  
Author(s):  
Laura Haynes ◽  
Nicholas Cohen
Keyword(s):  
T Cell ◽  
South African ◽  
Interleukin 2 ◽  
Biological Properties ◽  
Biologically Active ◽  
Lectin Affinity Chromatography ◽  
Sds Page ◽  
Cell Mitogen ◽  
Clawed Frog

A T-cell growth factor (TCGF) is produced by antigen- or mitogen-stimulated T lymphocytes from the South African clawed frogXenopus laevis. This study further defines the physical and biological properties of this cytokine and demonstrates that TCGF is biochemically similar to mammalian interleukin-2 (IL-2). Biologically active TCGF eluted from SDS-PAGE displays a Mrof 16 kD and lectin-affinity chromatography indicates that the three-dimensionmal configuration of carbohydrates on TCGF and human IL-2 is similar. Secretion of TCGF is detectable 1 day after stimulation of splenocytes with the T-cell mitogen phytohemagglutinin (PHA) and peaks following 2 to 3 days of stimulation. Finally, despite the biological and physical similarities betweenXenopusTCGF and mammalian IL-2, anti-human IL-2 monoclonal antibodies do not recognizeXenopusTCGF.

scholarly journals Analysis of Three Mutations in Italian Strains of SARS-CoV-2: Implications for Pathogenesis

Chemotherapy ◽  
2021 ◽  
pp. 1-5
Author(s):  
Domenico Benvenuto ◽  
Francesca Benedetti ◽  
Ayse Banu Demir ◽  
Massimo Ciccozzi ◽  
Davide Zella
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Vaccine Efficacy ◽  
Spike Protein ◽  
Enveloped Virus ◽  
Whole Genomes ◽  
The World ◽  
The Impact

Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped virus initially detected in Wuhan in December 2019, responsible for coronavirus disease 2019 (COVID-19), a respiratory syndrome currently affecting >220 countries around the world, with >80 million cases registered and >1.8 million deaths. Objective: As several vaccines are still being developed and 2 have been approved, it is particularly important to perform evolutionary surveillance to identify mutations potentially affecting vaccine efficacy. Methods: DynaMut server has been used to evaluate the impact of the mutation found on SARS-CoV-2 isolates available on GISAID. Results: In this article, we analyze whole genomes sequenced from Italian patients, and we report the characterization of 3 mutations, one of which presents in the spike protein. Conclusion: The mutations analyzed in this article can be useful to evaluate the evolution of SARS-CoV-2.

scholarly journals Epidemiology, incidence and mortality of oral cavity and lips cancer and their relationship with the human development index in the world

2016 ◽  
Vol 3 (10) ◽  
pp. 872-888 ◽  
Author(s):  
Fariba Ramezani Siakholak ◽  
Mahshid Ghoncheh ◽  
Reza Pakzad ◽  
Hamidreza Sadeghi Gandomani ◽  
Fereshteh Ghorat ◽  
...  
Keyword(s):  
Mortality Rate ◽  
Oral Cavity ◽  
Human Development ◽  
Human Development Index ◽  
Health Planning ◽  
Lip Cancer ◽  
Development Index ◽  
Incidence And Mortality ◽  
High Incidence ◽  
The World

Introduction: According to the importance of knowledge about incidence and mortality of oral cavity and lip cancer in health planning, this study was performed with the aim of investigating the incidence and mortality rate of oral cavity and lip cancer and its relation with the Human Development Index in the world in 2012. Methods: The study was conducted based on data from the world data of cancer and the World Bank (including the HDI and its components). Data about the age-specific incidence and mortality rate (ASR) for every country in 2012 were getting from the global cancer project. To analyze data, correlation tests between incidence and death rates, and HDI and its components were employed with a significance level of 0.05 using SPSS software. Results: In 2012, 300373 cases of oral cavity and lip cancer and 145353 cases of death from it have occurred in the world. A positive correlation of 0.221 was seen between the standardized incidence rate of oral cavity and lip cancer and HDI but this correlation was not statistically significant (p=0.114). On the other side, a correlation of 0.295 was seen between the standardized mortality rate of oral cavity and lip cancer with HDI that this correlation was statistically significant (p<0.001). Conclusion: The incidence and mortality of oral cavity cancer is high in the Asian countries especially south eastern of Asia. Performing preventive plans in high incidence and mortality rate regions and also obtaining etiological studies in these regions is recommended for diagnosing the causes of high incidence and mortality rates.

scholarly journals Large scale genomic analysis of 3067 SARS-CoV-2 genomes reveals a clonal geo-distribution and a rich genetic variations of hotspots mutations

2020 ◽  
Author(s):  
Meriem Laamarti ◽  
Tarek Alouane ◽  
Souad Kartti ◽  
M.W. Chemao-Elfihri ◽  
Mohammed Hakmi ◽  
...  
Keyword(s):  
Genetic Variants ◽  
Large Scale ◽  
Genomic Analysis ◽  
Geographic Location ◽  
Synonymous Mutations ◽  
Global Pandemic ◽  
Recurrent Mutations ◽  
Epidemic Period ◽  
The World

AbstractIn late December 2019, an emerging viral infection COVID-19 was identified in Wuhan, China, and became a global pandemic. Characterization of the genetic variants of SARS-CoV-2 is crucial in following and evaluating it spread across countries. In this study, we collected and analyzed 3,067 SARS-CoV-2 genomes isolated from 55 countries during the first three months after the onset of this virus. Using comparative genomics analysis, we traced the profiles of the whole-genome mutations and compared the frequency of each mutation in the studied population. The accumulation of mutations during the epidemic period with their geographic locations was also monitored. The results showed 782 variant sites, of which 512 (65.47%) had a non-synonymous effect. Frequencies of mutated alleles revealed the presence of 38 recurrent non-synonymous mutations, including ten hotspot mutations with a prevalence higher than 0.10 in this population and distributed in six SARS-CoV-2 genes. The distribution of these recurrent mutations on the world map revealed certain genotypes specific to the geographic location. We also found co-occurring mutations resulting in the presence of several haplotypes. Moreover, evolution over time has shown a mechanism of mutation co-accumulation which might affect the severity and spread of the SARS-CoV-2.On the other hand, analysis of the selective pressure revealed the presence of negatively selected residues that could be taken into considerations as therapeutic targetsWe have also created an inclusive unified database (http://genoma.ma/covid-19/) that lists all of the genetic variants of the SARS-CoV-2 genomes found in this study with phylogeographic analysis around the world.

Characterization of Portuguese Propolis: Unraveling Biological Properties

Planta Medica ◽  
2014 ◽  
Vol 80 (16) ◽  
Author(s):  
CA Aguiar ◽  
AM Ferreira ◽  
R Oliveira ◽  
F Baltazar ◽  
A Cunha
Keyword(s):  
Biological Properties

A Sustainable Approach for Organisational using Strategic Management

2020 ◽  
Vol 11 (2) ◽  
Author(s):  
Abdulla Almazrouei ◽  
◽  
Azlina Md Yassin ◽  
Keyword(s):  
Mortality Rate ◽  
Strategic Planning ◽  
Strategic Management ◽  
Crime Rate ◽  
External Environment ◽  
Police Department ◽  
Public Institutions ◽  
Abu Dhabi ◽  
The Public ◽  
The World

Strategic management have gained popularity in the public institutions to foster good delivery service to the public. The strategic planning enables organizations to establish a strategic match between the internal competency, resources and external environment. Majority of the successful organizations across the world use strategic management and planning as a tool that enables to optimize the operations and achieve maximum productivity with the resources. This paper reviewed on strategic management for organisations in Abu Dhabi especially for Abu Dhabi Police (ADP) force. It presents three strategic management theories which can be adopted by an organisation. This would help the organisation such as police department to reduce the increasing crime rate and mortality rate in UAE.

scholarly journals The absence of the caffeine synthase gene is involved in the naturally decaffeinated status of Coffea humblotiana, a wild species from Comoro archipelago

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Nathalie Raharimalala ◽  
Stephane Rombauts ◽  
Andrew McCarthy ◽  
Andréa Garavito ◽  
Simon Orozco-Arias ◽  
...  
Keyword(s):  
Wild Species ◽  
Illegitimate Recombination ◽  
Recombination Mechanism ◽  
Comparative Analyses ◽  
Robusta Coffee ◽  
Caffeine Synthase ◽  
The World ◽  
Decaffeinated Coffee ◽  
Chromosome Level

AbstractCaffeine is the most consumed alkaloid stimulant in the world. It is synthesized through the activity of three known N-methyltransferase proteins. Here we are reporting on the 422-Mb chromosome-level assembly of the Coffea humblotiana genome, a wild and endangered, naturally caffeine-free, species from the Comoro archipelago. We predicted 32,874 genes and anchored 88.7% of the sequence onto the 11 chromosomes. Comparative analyses with the African Robusta coffee genome (C. canephora) revealed an extensive genome conservation, despite an estimated 11 million years of divergence and a broad diversity of genome sizes within the Coffea genus. In this genome, the absence of caffeine is likely due to the absence of the caffeine synthase gene which converts theobromine into caffeine through an illegitimate recombination mechanism. These findings pave the way for further characterization of caffeine-free species in the Coffea genus and will guide research towards naturally-decaffeinated coffee drinks for consumers.

Sign in / Sign up

Export Citation Format

Share Document