Anti-Inflammatory Potential of the Oleoresin from the Amazonian Tree Copaifera reticulata with an Unusual Chemical Composition in Rats

Copaifera reticulata Ducke is a popularly known species known as copaíba that is widely spread throughout the Amazon region. The tree yields an oleoresin which is extensively used in local traditional medicine mainly as an anti-inflammatory and antinociceptive agent. The aim of the present study was to assess the anti-inflammatory potential of this oleoresin obtained from a national forest in the central Amazon which presented an unusual chemical composition. The chemical composition of volatile compounds of oleoresin was analyzed by gas chromatography-mass spectrometry. The acute toxicity assay was performed with a single dose of 2000 mg/kg. The anti-inflammatory potential was evaluated by carrageenan-induced paw edema and air pouch assays using four different C. reticulata oleoresin concentrations (10, 100, and 400 mg/kg). The exudate was evaluated for nitrite concentration through the colorimetric method and for TNF-α, IL-1β, and PGE2 by ELISA. C. reticulata oleoresin collected in the Amazonian summer contained six major sesquiterpene compounds (β-bisabolene, cis-eudesma-6,11-diene, trans-α-bergamotene, β-selinene, α-selinene, and β-elemene) and was nontoxic at a dose of 2000 mg/kg, showing low acute toxicity. Different from oleoresin obtained from other sites of the Brazilian Amazon, the major volatile compound found was β-Bisabolene with 25.15%. This β-Bisabolene-rich oleoresin reduced the formation of paw edema induced by carrageenan and reduced the global number of cells in the air pouch assay, as well as exudate volume and nitrite, TNF-α, IL-1β, and prostaglandin E2 levels (p < 0.05). C. reticulata oleoresin with a high β-Bisabolene concentration showed anti-inflammatory activity, reducing vascular permeability and consequently edema formation, and thus reducing cell migration and the production of inflammatory cytokine, confirming its traditional use by local Amazonian communities.

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TRPV1 Blocker HCRG21 Suppresses TNF-α Production and Prevents the Development of Edema and Hypersensitivity in Carrageenan-Induced Acute Local Inflammation

Biomedicines ◽

10.3390/biomedicines9070716 ◽

2021 ◽

Vol 9 (7) ◽

pp. 716

Author(s):

Oksana Sintsova ◽

Irina Gladkikh ◽

Anna Klimovich ◽

Yulia Palikova ◽

Viktor Palikov ◽

...

Keyword(s):

Transient Receptor Potential ◽

Thermal Hyperalgesia ◽

Control Group ◽

Transient Receptor Potential Vanilloid ◽

Paw Edema ◽

Mice Model ◽

Edema Formation ◽

Sensitivity Experiment ◽

Tnf Α ◽

Anti Inflammatory

Currently the TRPV1 (transient receptor potential vanilloid type 1) channel is considered to be one of the main targets for pro-inflammatory mediators including TNF-α. Similarly, the inhibition of TRPV1 activity in the peripheral nervous system affects pro-inflammatory mediator production and enhances analgesia in total. In this study, the analgesic and anti-inflammatory effects of HCRG21, the first peptide blocker of TRPV1, were demonstrated in a mice model of carrageenan-induced paw edema. HCRG21 in doses of 0.1 and 1 mg/kg inhibited edema formation compared to the control, demonstrated complete edema disappearance in 24 h in a dose of 1 mg/kg, and effectively reduced the productionof TNF-α in both doses examined. ELISA analysis of blood taken 24 h after carrageenan administration showed a dramatic cytokine value decrease to 25 pg/mL by HCRG21 versus 100 pg/mL in the negative control group, which was less than the TNF-α level in the intact group (40 pg/mL). The HCRG21 demonstrated potent analgesic effects on the models of mechanical and thermal hyperalgesia in carrageenan-induced paw edema. The HCRG21 relief effect was comparable to that of indomethacin taken orally in a dose of 5 mg/kg, but was superior to this nonsteroidal anti-inflammatory drug (NSAID) in duration (which lasted 24 h) in the mechanical sensitivity experiment. The results confirm the existence of a close relationship between TRPV1 activity and TNF-α production once again, and prove the superior pharmacological potential of TRPV1 blockers and the HCRG21 peptide in particular.

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Chemical Composition and Anti-inflammation Activity of Essential Oils from Citrus unshiu Flower

Natural Product Communications ◽

10.1177/1934578x1400900538 ◽

2014 ◽

Vol 9 (5) ◽

pp. 1934578X1400900 ◽

Cited By ~ 2

Author(s):

Min-Jin Kim ◽

Kyong-Wol Yang ◽

Sang Suk Kim ◽

Suk Man Park ◽

Kyung Jin Park ◽

...

Keyword(s):

Chemical Composition ◽

Essential Oils ◽

Biological Activities ◽

Chemical Constituents ◽

Gas Chromatography Mass Spectrometry ◽

Raw 264.7 Cells ◽

Prostaglandin E ◽

Raw 264.7 ◽

Tnf Α ◽

Anti Inflammatory

Though many essential oils from citrus peels are claimed to have several medicinal functions, the chemical composition and biological activities of the essential oils of Citrus flowers have not been well described. Therefore, this study intended to investigate the chemical composition and anti-inflammatory potential of essential oils from C. unshiu flower (CEO) to support its purported beneficial health effects. The chemical constituents of the CEO, analyzed by gas chromatography-mass spectrometry (GC-MS), included γ-terpinene (24.7%), 2-β-pinene (16.6%), 1-methyl-2-isopropylbenzene (11.5%), L-limonene (5.7%), β-ocimene (5.6%), and α-pinene (4.7%). The effects of the CEO on nitric oxide (NO) and prostaglandin E2 (PGE2) production in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages were also examined. The results indicate that the CEO is an effective inhibitor of LPS-induced NO and PGE2 production in RAW 264.7 cells. Additionally, CEO was shown to suppress the production of inflammatory cytokines including interleukin (IL)-1β, tumor necrosis factor (TNF)-α, and IL-6. Based on these results, CEO may be considered a potential anti-inflammatory candidate with human health benefits.

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Chemical Composition of the Essential Oils of Pogostemon auricularius, a Vietnamese Medicinal Plant

Natural Product Communications ◽

10.1177/1934578x1801300524 ◽

2018 ◽

Vol 13 (5) ◽

pp. 1934578X1801300 ◽

Cited By ~ 1

Author(s):

Prabodh Satyal ◽

Nguyen Thi Hong Chuong ◽

Van The Pham ◽

Nguyen Huy Hung ◽

Vu Thi Hien ◽

...

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Chemical Composition ◽

Medicinal Plant ◽

Essential Oils ◽

Sore Throat ◽

Gas Chromatography Mass Spectrometry ◽

Traditional Use ◽

Chromatography Mass Spectrometry ◽

Anti Inflammatory

The essential oils from the stems, the leaves, and the flowers of Pogostemon auricularius, growing wild in Vietnam, were obtained by hydrodistillation and analyzed by gas chromatography – mass spectrometry. The essential oils were dominated by sesquiterpenoids (62.3–71.0%) and diterpenoids (23.9–27.3%), with β-caryophyllene (20.7–31.5%), α-humulene (8.9–14.1%), and ( E,E)-geranyl linalool (14.0–19.6%) as major components. The abundant concentrations of β-caryophyllene and α-humulene, known analgesic and anti-inflammatory agents, likely account for the traditional use of P. auricularius in Vietnam to treat fevers, malaria, and sore throat.

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Chemical Composition, Antimicrobial and Anti-inflammatory Activity of Algerian Juniperus phoenicea Essential Oils

Natural Product Communications ◽

10.1177/1934578x1801300227 ◽

2018 ◽

Vol 13 (2) ◽

pp. 1934578X1801300

Author(s):

Wafae Abdelli ◽

Fouad Bahri ◽

Martina Höferl ◽

Juergen Wanner ◽

Erich Schmidt ◽

...

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Acute Toxicity ◽

Essential Oils ◽

Volatile Compounds ◽

Steam Distillation ◽

Paw Edema ◽

Juniperus Phoenicea ◽

Anti Inflammatory ◽

Total Oil

The essential oils (EOs) obtained by steam distillation from the leaves and the berries of Juniperus phoenicea L., harvested in northwest of Algeria were analyzed and their antimicrobial and anti-inflammatory activities were assessed. 63, 46 and 78 volatile compounds were identified by GC-FID and GC-MS from fresh leaves, dried leaves and berries representing 98.1%, 98.3% and 96.4% of the total oil, respectively. The fresh and dried leaves oils were mainly composed of β-phellandrene (43.9% / 44.9%), α-pinene (25.1% / 20.3%), myrcene (8.5% / 8.2%), α-phellandrene (4.7% / 4.5%) p -cymene (2.7%-3.0%) and limonene (2.3%-2.5%) whereas, the berries oil was mainly composed of α-pinene (43.7%), p -cymene (5.8%), β-phellandrene (4.6%), α-terpineol (4.3%) and α-campholenal (4.0%). The study of the antimicrobial activity showed that the 3 EOs were effective only on B. cereus ATCC 10876 and C. albicans ATCC 10231. No signs of acute toxicity have been noted in mice even at the highest dose tested (5000 mg/kg p.o). The fresh leaves, dried leaves and berries oils reduced the carrageenan-induced paw edema in mice by 16.8%, 15.2% and 6.4%, respectively, after 6 hours at a dose of 400 mg/kg p.o.

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Anti-inflammatory Effect of Woodfordia fructicosa Leaves Ethanolic Extract on Adjuvant and Carragenan Treated Rats

Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry ◽

10.2174/1871523018666190222120127 ◽

2020 ◽

Vol 19 (2) ◽

pp. 103-112

Author(s):

Hem Raj ◽

Avneet Gupta ◽

Neeraj Upmanyu

Keyword(s):

Animal Models ◽

Ethanolic Extract ◽

Inhibitory Effect ◽

Inflammatory Activity ◽

Paw Edema ◽

Sprague Dawley ◽

Traditional Use ◽

Necrosis Factor Alpha ◽

Tnf Α ◽

Anti Inflammatory

Background: Woodfordia fructicosa is used traditionally for the treatment of inflammation associated with arthritis. Methods: In the present study, the anti-inflammatory activity of W. fructicosa (WFE) leaves ethanolic extract was assessed in Sprague Dawley rats by giving 200 mg/kg dose orally. Inflammation was studied by using carrageenan induced paw edema, Freund’s adjuvant (FA) and monosodium iodo acetate (MIA) induced arthritis as animal models. Serum tumor necrosis factor-alpha (TNF-α) was estimated in blood sample of animals treated with FA. The one way ANOVA followed by Bonferroni’s test was used for statistical analysis. Results: WFE significantly decreased (P<0.05, P<0.001) paw thickness in carrageenan induced paw edema and FA induced arthritis. The significant decrease in knee diameter (P<0.001) in MIA induced arthritis as well as inhibitory effect (P<0.001) on elevated TNF- α was observed. Conclusion: These results showed that the WFEexerted an inhibitory effect on TNF-α and carrageenan paw edema which may justify its traditional use in inflammatory conditions. Thus, the study shows that leaves of W. fruticose afford anti-inflammatory activity by preventing the inflammation in different animal models.

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Chemical Composition and Anti-inflammatory Activity of Algerian Thymus vulgaris Essential Oil

Natural Product Communications ◽

10.1177/1934578x1701200435 ◽

2017 ◽

Vol 12 (4) ◽

pp. 1934578X1701200 ◽

Cited By ~ 6

Author(s):

Wafae Abdelli ◽

Fouad Bahri ◽

Abderrahmane Romane ◽

Martina Höferl ◽

Juergen Wanner ◽

...

Keyword(s):

Chemical Composition ◽

Acute Toxicity ◽

Essential Oil ◽

Inflammatory Activity ◽

Thymus Vulgaris ◽

Paw Edema ◽

Thyme Oil ◽

Anti Inflammatory ◽

Total Oil

This work aims to elucidate the chemical composition of two essential oil (EO) samples obtained from the leaves of Thymus vulgaris L. ( Lamiaceae) collected in two regions of Northwestern Algeria (Tlemcen and Mostaganem) and to assess their in vivo acute toxicity and anti-inflammatory activity. Sixty-six compounds could be identified by means of simultaneous GC-FID and GC-MS, accounting for 99.3% of total thyme oil of Mostaganem (EO.TM) and 99.0% of Tlemcen (EO.TT). In both samples, thymol was the major component, amounting to 59.5% (EO.TM) and 67.3% (EO.TT) of the total oil. EO.TT proved to be acutely toxic to mice at a dose of 4500 mg/kg p.o., whereas EO.TM did not show signs of acute toxicity, even at the highest dose tested (5000 mg/kg p.o.). Both EO samples were proven to possess anti-inflammatory activities, significantly reducing carrageenan-induced paw edema in mice (after 6 hours at a dose of 400 mg/kg p.o) at 58.4% for EO.TT and 50.4% for EO.TM, respectively. In conclusion, it could be demonstrated that EOs of T. vulgaris exhibit a considerable in vivo anti-inflammatory activity at non-toxic doses.

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Anti-inflammatory, analgesic, and acute toxicity evaluation of Litchi chinensis seed extract in albino rat

10.53365/nrfhh/141230 ◽

2021 ◽

Author(s):

Sindhu KC ◽

Bipindra Pandey ◽

Sistu KC ◽

Santosh Gurung ◽

Ashish Gautam

Keyword(s):

Acute Toxicity ◽

Seed Extract ◽

Albino Rats ◽

Paw Edema ◽

Hot Plate ◽

Traditional Use ◽

Albino Rat ◽

Litchi Chinensis ◽

Rat Paw Edema ◽

Anti Inflammatory

Litchi chinensis is a edible fruits of soapberry family commonly known as lychee; is traditionally used for the treatment of inflammation, headache, and body pain. The present study investigated the toxicity, anti-inflammatory, and analgesic activity of the ethanolic crude extract of Litchi chinensis to support its traditional use in its folk medicine and to screen the phytochemical constituents. 70% ethanolic extract of seeds of Litchi chinensis (LCSE) was prepared and preliminary phytochemical screening was performed. Acute toxicity of LCSE was carried out based on OECD guidelines 423. In vivo anti-inflammatory and analgesic effect of the extract was evaluated using carrageenan-induced paw edema and hot plate methods in Wistar albino rat's model. Results revealed that LCSE contains phenols, tannins, flavonoids, saponins, steroids, and alkaloids. LD50 values were found to be higher than 5000 mg/kg body weight; no any sign of toxicity, behavior changes, moribund, and mortality were observed in LCSE treated animals. Oral administration of LCSE at the dose of 150, 300, and 600 mg/kg produced a significant (p<0.05) dose-dependent inhibition in carrageenan-induced rat paw edema and hot plate. These results suggest that LCSE is non-toxic, and shows potent anti-inflammatory and analgesic activities over Wistar albino rats. These finding demonstrate that lychee seed extract acts as a good therapeutic candidate for the safe anti-inflammatory agents.

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Reduction of paw edema and liver oxidative stress in carrageenan-induced acute inflammation by Lobaria pulmonaria and Parmelia caperata, lichen species, in mice

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000620 ◽

2019 ◽

pp. 1-9

Author(s):

Samira Salem ◽

Essaid Leghouchi ◽

Rachid Soulimani ◽

Jaouad Bouayed

Keyword(s):

Time Course ◽

Acute Inflammation ◽

Lichen Species ◽

Paw Edema ◽

Sod Activity ◽

Edema Formation ◽

Lobaria Pulmonaria ◽

Anti Inflammatory ◽

Endogenous Antioxidant ◽

Inflammatory Effect

Abstract. Paw edema volume reduction is a useful marker in determining the anti-inflammatory effect of drugs and plant extracts in carrageenan-induced acute inflammation. In this study, the anti-inflammatory effect of Lobaria pulmonaria (LP) and Parmelia caperata (PC), two lichen species, was examined in carrageenan-induced mouse paw edema test. Compared to the controls in carrageenan-induced inflammation (n = 5/group), our results showed that pretreatment by single oral doses with PC extract (50–500 mg/kg) gives better results than LP extract (50–500 mg/kg) in terms of anti-edematous activity, as after 4 h of carrageenan subplantar injection, paw edema formation was inhibited at 82–99% by PC while at 35–49% by LP. The higher anti-inflammatory effect of PC, at all doses, was also observed on the time-course of carrageenan-induced paw edema, displaying profile closely similar to that obtained with diclofenac (25 mg/kg), an anti-inflammatory drug reference (all p < 0.001). Both LP and PC, at all doses, significantly ameliorated liver catalase (CAT) activity (all p < 0.05). However, superoxide dismutase (SOD) activity, glutathione peroxidase (GPx) activity and glutathione (GSH) levels were found increased in liver of PC- compared to LP-carrageenan-injected mice. Our findings demonstrated on one hand higher preventive effects of PC compared to LP in a mouse carrageenan-induced inflammatory model and suggested, on the other hand, that anti-inflammatory effects elicited by the two lichens were closely associated with the amelioration in the endogenous antioxidant status of liver.

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Anti-inflammatory Effects ofScoparia dulcisL. and Betulinic Acid

The American Journal of Chinese Medicine ◽

10.1142/s0192415x11009329 ◽

2011 ◽

Vol 39 (05) ◽

pp. 943-956 ◽

Cited By ~ 38

Author(s):

Jen-Chieh Tsai ◽

Wen-Huang Peng ◽

Tai-Hui Chiu ◽

Shang-Chih Lai ◽

Chao-Ying Lee

Keyword(s):

Betulinic Acid ◽

High Performance ◽

Ethanol Extract ◽

Paw Edema ◽

Scoparia Dulcis ◽

Inflammatory Mechanism ◽

Tnf Α ◽

Cox 2 ◽

Anti Inflammatory ◽

Analytical Result

The aims of this study intended to investigate the anti-inflammatory activity of the 70% ethanol extract from Scoparia dulcis (SDE) and betulinic acid on λ-carrageenan-induced paw edema in mice. The anti-inflammatory mechanism of SDE and betulinic acid was examined by detecting the levels of cyclooxygenase-2 (COX-2), nitric oxide (NO), tumor necrosis factor (TNF-α), interleukin-1β (IL-1β) and malondialdehyde (MDA) in the edema paw tissue and the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione reductase (GRd) in the liver. The betulinic acid content in SDE was detected by high performance liquid chromatography (HPLC). In the anti-inflammatory model, the results showed that SDE (0.5 and 1.0 g/kg) and betulinic acid (20 and 40 mg/kg) reduced the paw edema at 3, 4 and 5 h after λ-carrageenan administration. Moreover, SDE and betulinic acid affected the levels of COX-2, NO, TNF-α and IL1-β in the λ-carrageenan-induced edema paws. The activities of SOD, GPx and GRd in the liver tissue were increased and the MDA levels in the edema paws were decreased. It is suggested that SDE and betulinic acid possessed anti-inflammatory activities and the anti-inflammatory mechanisms appear to be related to the reduction of the levels of COX-2, NO, TNF-α and IL1-β in inflamed tissues, as well as the inhibition of MDA level via increasing the activities of SOD, GPx and GRd. The analytical result showed that the content of betulinic acid in SDE was 6.25 mg/g extract.

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Chemical Composition, Allelopathic, Antioxidant, and Anti-Inflammatory Activities of Sesquiterpenes Rich Essential Oil of Cleome amblyocarpa Barratte & Murb.

Plants ◽

10.3390/plants10071294 ◽

2021 ◽

Vol 10 (7) ◽

pp. 1294

Author(s):

Ahmed M. Abd-ElGawad ◽

Abdelbaset M. Elgamal ◽

Yasser A. EI-Amier ◽

Tarik A. Mohamed ◽

Abd El-Nasser G. El Gendy ◽

...

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Climatic Conditions ◽

Chemometric Analysis ◽

Gas Chromatography Mass Spectrometry ◽

Authentic Materials ◽

Dactyloctenium Aegyptium ◽

Hexahydrofarnesyl Acetone ◽

Ic50 Values ◽

Anti Inflammatory

The integration of green natural chemical resources in agricultural, industrial, and pharmaceutical applications allures researchers and scientistic worldwide. Cleome amblyocarpa has been reported as an important medicinal plant. However, its essential oil (EO) has not been well studied; therefore, the present study aimed to characterize the chemical composition of the C. amblyocarpa, collected from Egypt, and assess the allelopathic, antioxidant, and anti-inflammatory activities of its EO. The EO of C. amblyocarpa was extracted by hydrodistillation and characterized via gas chromatography–mass spectrometry (GC-MS). The chemometric analysis of the EO composition of the present studied ecospecies and the other reported ecospecies was studied. The allelopathic activity of the EO was evaluated against the weed Dactyloctenium aegyptium. Additionally, antioxidant and anti-inflammatory activities were determined. Forty-eight compounds, with a prespondence of sesquiterpenes, were recorded. The major compounds were caryophyllene oxide (36.01%), hexahydrofarnesyl acetone (7.92%), alloaromadendrene epoxide (6.17%), myrtenyl acetate (5.73%), isoshyobunone (4.52%), shyobunol (4.19%), and trans-caryophyllene (3.45%). The chemometric analysis revealed inconsistency in the EO composition among various studied ecospecies, where it could be ascribed to the environmental and climatic conditions. The EO showed substantial allelopathic inhibitory activity against the germination, seedling root, and shoot growth of D. aegyptium, with IC50 values of 54.78, 57.10, and 74.07 mg L−1. Additionally, the EO showed strong antioxidant potentiality based on the IC50 values of 4.52 mg mL−1 compared to 2.11 mg mL−1 of the ascorbic acid as standard. Moreover, this oil showed significant anti-inflammation via the suppression of lipoxygenase (LOX) and cyclooxygenases (COX1, and COX2), along with membrane stabilization. Further study is recommended for analysis of the activity of pure authentic materials of the major compounds either singularly or in combination, as well as for evaluation of their mechanism(s) and modes of action as antioxidants or allelochemicals.

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