scholarly journals The result of effective microorganisms isolated from wastewater and soil

2021 ◽  
Vol 32 (1) ◽  
pp. 38-44
Author(s):  
Oyunbileg Natsagdorj ◽  
Indra Nyamjav ◽  
Byambasuren Davaasambuu ◽  
Ariunzaya Jargalsaikhan ◽  
Batsukh Chultem
Keyword(s):  
Rhodobacter Sphaeroides ◽  
Heavy Metal Contamination ◽  
Biologically Active ◽  
Molecular Characteristics ◽  
Isolation And Identification ◽  
Effective Microorganisms ◽  
Streptomyces Sp ◽  
Streptomyces Spp ◽  
Rdna Sequencing ◽  
Effective Microorganism

Increasing urbanization and population concentrations are causing environmental problems such as soil and water pollution. In this study, we were present isolation and identification of Streptomyces sp and Rhodobacteria sp. Those effective microorganisms isolated from the wastewater and soil sample of Anand-Khujirt spring resort located in Khujirt soum, Uvurkhangai province in Mongolia. Based on morphological, cultural, and molecular characteristics (16S rDNA sequencing), those strain were identified as Rhodobacter sphaeroides (99% similarity) and Streptomyces cf.griseus (99% similarity). Rhodobacter sphaeroides could be used to reduce heavy metal contamination from soil. Furthermore, Streptomyces cf.griseus is an effective  microorganism, which produces 32 types of biologically active compounds for pharmaceutical or agricultural purposes. Хаягдал ус, хөрснөөс ашигтай бичил биетний цэвэр өсгөвөр ялгасан судалгааны дүнгээс Хотжилт, хүн амын төвлөрөл нэмэгдэж байгаа нь хөрс, усны бохирдол зэрэг хүрээлэн буй орчны асуудлуудыг үүсгэж байна. Бид энэхүү судалгааны ажлаар Өвөрхангай аймгийн Хужирт сумын нутагт орших “Ананд-Хужирт” рашаан сувилал,  аялал жуулчлалын баазын хаягдал ус, хөрснөөс ялгасан ашигтай бичил биетний нутгийн омгууд болох Streptomyces sp, Rhodobacteria sp-ийн нуклеотидын дарааллыг Genetyx software програмыг ашиглан боловсруулалт хийн АНУ-ын Биотехнологийн Мэдээллийн Үндэсний Төвийн мэдээллийн сангаас BLAST хайлт хийж хамгийн ойролцоох зүйлийг тодорхойлсон. Rhodobacter sphaeroides (99% ижил нуклеотидтэй)-ийг хүнд металлын бохирдлыг бууруулахад өргөн ашигладаг бол Streptomyces cf.griseus (99% ижил нуклеотидтэй) нь 32 төрлийн биологийн идэвхит нэгдлийг нийлэгжүүлэгч ашигтай бичил биетэн юм.    Түлхүүр үг: Streptomyces spp, Rhodobacteria spp, хаягдал ус, хөрс,

scholarly journals Antioxidant, antimicrobial and cytotoxic activities of secondary metabolites from Streptomyces sp. isolated of the Amazon-Brazil region

2021 ◽  
Vol 10 (10) ◽  
pp. e366101018974
Author(s):  
Sandrine Maria Arruda Lima ◽  
Pedro Silvino Pereira ◽  
Bruno Iraquitan Miranda da Silva ◽  
Natalie Emanuelle Ribeiro ◽  
Elizabeth Fernanda de Oliveira Borba ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Antimicrobial Activities ◽  
Biologically Active ◽  
Cytotoxic Activities ◽  
Cytotoxicity Test ◽  
Peripheral Blood Mononuclear ◽  
Streptomyces Sp ◽  
Streptomyces Spp ◽  
Promising Source

Bacteria of Streptomyces genus are a promising source of biologically active products, with applications in medicine, industry and agriculture. Therefore, the objective of this work was to evaluate the cytotoxic, antioxidant and antimicrobial activities of fermented rice extract and their semipurified fractions from Streptomyces spp. isolated of the rhizosphere of Paullinia cupana, Amazon-Brazil. For this, a bioguided study was carried out by the cytotoxic activity with methanolic extract of Streptomyces sp. ACTMS-12H UFPEDA 3405 (EMeOH-12H) partitioned with n-hexane, ethyl acetate and 2-butanol. The antioxidant activity was analyzed using the DPPH, ABTS and phosphomolybdenum methods, while the antimicrobial activity was investigated by microdilution method to determine the minimum inhibitory concentration (MIC) against species of bacteria and yeast. In the cytotoxicity test, the butanolic phase (FbuOH-12H) presented IC50 of 1.1 µg/mL against MOLT-4, with cell death probably by apoptosis, but did not cause cytotoxicity on peripheral blood mononuclear cell (PBMC) or human erythrocytes. Chemical prospecting detected the presence of saponins and reducing sugars on 2-butanol fraction (FBuOH-12H), which can be related to cytotoxicity. On the antioxidant activity by ABTS, the partition with ethyl acetate (FAcOEt-12H) showed antioxidant capacity of 1161.7 ± 0.04 µM of Trolox/g of extract, indicating an expressive reactivity of the phase with this radical. The aqueous phases (from hexane, ethyl acetate and methanol extracts) were active in all tested microorganisms, except E. faecalis.

scholarly journals Isolation and Identification of Streptomyces spp. from Desert and Savanna Soils in Sudan

2020 ◽  
Vol 17 (23) ◽  
pp. 8749
Author(s):  
Mohamed E. Hamid ◽  
Adil Mahgoub ◽  
Abdulrhman J. O. Babiker ◽  
Hussein A. E. Babiker ◽  
Mohammed A. I. Holie ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Antibiotic Production ◽  
Rrna Gene ◽  
Isolation And Identification ◽  
Streptomyces Sp ◽  
Ecological Zones ◽  
Streptomyces Spp ◽  
Gene Sequence Analysis ◽  
Unique Species

The purpose of this study was to investigate streptomycete populations in desert and savanna ecozones in Sudan and to identify species based on 16S rRNA gene sequences. A total of 49 different Streptomyces phenotypes (22 from sites representing the desert and semi-desert ecozone; 27 representing the savanna ecozone) have been included in the study. The isolates were characterized phenotypically and confirmed using 16S rRNA gene sequence analysis. The two ecozones showed both similarities and uniqueness in the types of isolates. The shared species were in cluster 1 (Streptomyces (S.) werraensis), cluster 2 (Streptomyces sp.), cluster 3 (S. griseomycini-like), and cluster 7 (S. rochei). The desert ecozone revealed unique species in cluster 9 (Streptomyces sp.) and cluster 10 (S. griseomycini). Whereas, the savanna ecozone revealed unique species in cluster 4 (Streptomyces sp.), cluster 5 (S. albogriseolus/ S. griseoincarnatus), cluster 6 (S. djakartensis), and cluster 8 (Streptomyces sp.). Streptomycetes are widely distributed in both desert and the savanna ecozones and many of these require full descriptions. Extending knowledge on Streptomyces communities and their dynamics in different ecological zones and their potential antibiotic production is needed.

scholarly journals Pembuatan Kompos Sampah Dapur dan Taman dengan Bantuan Aktivator EM4

2021 ◽  
Vol 6 ◽  
pp. 18
Author(s):  
Sri Hastuti ◽  
Tri Martini ◽  
Candra Purnawan ◽  
Abu Masykur ◽  
Atmanto Heru Wibowo
Keyword(s):  
Organic Matter ◽  
Lactic Acid ◽  
Fermentation Process ◽  
Photosynthetic Bacteria ◽  
Plant Roots ◽  
Effective Microorganisms ◽  
Fermentation Time ◽  
Streptomyces Sp ◽  
Effective Microorganism ◽  
Garden Waste

<p>Pembuatan kompos dari sampah dapur dan taman dengan bantuan <em>effective microorganism</em> (EM4) dan <em>microorganism local</em> (MOL) telah dilakukan. Tujuan dari kegiatan ini adalah memanfaatkan sampah yang ada di sekitar untuk dijadikan barang yang lebih berguna.  EM4 merupakan kultur campuran dari mikroorganisme yang menguntungkan yang mengandung mikroorganisme fermentasi dan sintetik yang terdiri dari bakteri Asam Laktat (<em>Lactobacillus Sp</em>), bakteri Fotosentetik (<em>Rhodopseudomonas Sp</em>), <em>Actinomycetes Sp</em>, <em>Streptomyces Sp</em> dan Yeast (ragi) dan Jamur pengurai selulose. Bahan ini membantu fermentasi bahan organik tanah menjadi senyawa organik yang mudah diserap oleh akar tanaman.  Proses pembuatan kompos dilakukan dengan mencampurkan sampah dapur dan taman dengan penambahan EM4. Proses fermentasi dilakukan variasi waktu 10, 14, 21, 26 dan 32 hari. Hasil pengamatan menunjukkan bahwa semakin lama fermentasi kompos yang dihasilkan semakin baik dimana daun telah hancur berubah bentuk seperti tanah.</p><p><strong><em>Kitchen and Garden Waste Composting using EM4 Activator. </em></strong><em>Composting of kitchen and garden waste with the help of effective microorganisms (EM4) and microorganism local (MOL) has been carried out. The purpose of this activity is to use the waste to become more useful items. EM4 is a mixed culture of beneficial microorganisms. This material contains microorganisms consisting of lactic acid bacteria (Lactobacillus Sp), photosynthetic bacteria (Rhodopseudomonas Sp), Actinomycetes Sp, Streptomyces Sp, and yeast, and cellulose-decomposing fungi. This activator helps break down soil organic matter into organic compounds that are easily absorbed by plant roots. The composting was done by mixing kitchen and garden waste with the addition of EM4 and MOL. The fermentation process was carried out in variations of 10, 14, 21, 26, and 32 days. The results showed that the longer the fermentation time the better the compost was produced indicating by the leaves had crumbled into shape like the soil.</em></p>

scholarly journals ISOLATION AND IDENTIFICATION OF Streptomyces spp. PRODUCING CHITINASE ENZYME FROM SEWAGE MUD

2018 ◽  
Vol 5 (1) ◽  
pp. 99
Author(s):  
Putu Ayu Parwati ◽  
Retno Kawuri ◽  
Ni Luh Watiniasih
Keyword(s):  
Isolation And Identification ◽  
Streptomyces Sp ◽  
Streptomyces Spp

Streptomyces merupakan bakteri dari kelompok Actinomycetes. Genus ini diketahui dapat memproduksi berbagai senyawa aktif diantaranya antibiotik, antiviral, dan enzim. Enzim yang dapat ditemukan pada bakteri dan jamur yaitu salah satunya enzim kitinase. Enzim kitinase yang diproduksi oleh bakteri lebih baik dibandingkan kitinase dari sumber lain karena dengan waktu yang relatif singkat dapat melakukan proses perkembangbiakan. Bakteri kitinolitik merupakan agen pengendalian hayati terhadap jamur patogen maupun serangga hama yang sangat potensial karena aktivitas kitinase yang dimiliki. Penelitian ini bertujuan untuk mengisolasi, mengetahui karakteristik Streptomyces spp. dari lumpur selokan dan uji aktivitas enzim kitinase. Aktivitas enzim kitinase diketahui melalui zona bening yang terbentuk pada media uji. Hasil penelitian diperoleh lima spesies Streptomyces spp. dari  sampel lumpur selokan yang diisolasi dengan karakteristik yang berbeda-beda. Hasil penelitian juga menunjukkan bahwa dari lima spesies Streptomyces spp., dua spesies yaitu Streptomyces sp.3 dan Streptomyces sp.5 mampu memproduksi enzim kitinase, sementara tiga spesies Streptomyces lainnya tidak memproduksi enzim kitinase.  Streptomyces sp.3 memproduksi enzim kitinase dengan terbentuknya zona bening sebesar 1,85±0,07 cm dan pada Streptomyces sp.5 terbentuk zona bening sebesar 0,65 ±0,14 cm . Berdasarkan data tersebut Streptomyces sp.3 dan Streptomyces sp.5 memiliki potensi sebagai bakteri kitinolitik yang memiliki manfaat sebagai agen pengendalian hayati.

scholarly journals Isolation and identification of microorganisms for polyurethane degradation

2019 ◽  
pp. 57-66
Author(s):  
Julie Tan ◽  
Takuji Ohwada
Keyword(s):  
Bacillus Pumilus ◽  
Environmental Problem ◽  
Soil Samples ◽  
Base Sequence ◽  
Pseudomonas Sp ◽  
Isolation And Identification ◽  
Streptomyces Sp ◽  
Streptomyces Spp ◽  
Sequence Patterns ◽  
Polyurethane Degradation

Plastic wastes decomposition has been a pressing environmental problem worldwide. In this study, polyurethane (PUR), a thermoset plastic was tested for biodegradation by polyurethane-degrading microorganisms that were isolated from a dumpsite at Kamishihoro, Tokachi, Obihiro, Japan. Actinomycetes were the most abundant microorganisms from soil samples. From the 65 isolated microbial species, 16 possessed polyurethane-degrading ability. These isolates exhibited clearing zones on Yeast-extract salts + Agar and gelatin with polyurethane (YES-AG + PUR). The PUR-degrading isolates were characterized and identified based on their DNA sequence patterns. Some isolates belong to the same genus or species. They were Bacillus chitinolyticus (B03, B04, B07), Streptomyces spp. (B13, B19, C13a, C15, C17a, C17b), Pseudomonas sp. (B20), Bacillus pumilus(B21), Streptomyces cuspidosporus (C10b, C18, C19) and Pseudallesscheria baydii (F04, F07). Streptomyces sp. coded as C13a, with base sequence homology of 99.7% with Streptomyces albogriseolus, was believed to produce the highest amount of both exo- and endo polyurethanases. This was demonstrated by the widest clearing zones when broth and cell-bound supernatants were inoculated into the YES-AG + PUR plate.

Metabolite fingerprinting of novel Streptomyces UK-238 from the Himalayan forest

2020 ◽  
Vol 16 ◽  
Author(s):  
Nidhi Srivastava ◽  
Indira P. Sarethy
Keyword(s):  
Ethyl Acetate ◽  
16S Rdna ◽  
Retention Index ◽  
Ethyl Acetate Extract ◽  
Similarity Index ◽  
Antimicrobial Drug ◽  
16S Rdna Sequencing ◽  
Metabolite Fingerprinting ◽  
Streptomyces Sp ◽  
Rdna Sequencing

Aims: Characterization of antimicrobial metabolites of novel Streptomyces sp. UK-238. Background: Novel antimicrobial drug discovery is urgently needed due to emerging multi antimicrobial drug resistance among pathogens. Since many years, natural products have provided the basic skeletons for many therapeutic compounds including antibiotics. Bioprospection of un/under explored habitats and focussing on selective isolation of actinobacteria as major reservoir of bio and chemodiversity has yielded good results. Objective: The main objectives of the study were the identification of UK-238 by 16S rDNA sequencing and antimicrobial metabolite fingerprinting of culture extracts. Method: In the present study, a promising isolate, UK-238, has been screened for antimicrobial activity and metabolite fingerprinting from the Himalayan Thano Reserve forest. It was identified by 16S rDNA sequencing. Ethyl acetate extract was partially purified by column chromatography. The pooled active fractions were fingerprinted by GC-MS and compounds were tentatively identified by collated data analysis based on Similarity Index, observed Retention Index from Databases and calculated Retention Index. Results: UK-238 was identified as Streptomyces sp. with 98.4% similarity to S. niveiscabiei. It exhibited broad-spectrum antibacterial and antifungal activity. GC-MS analysis of active fractions of ethyl acetate extract showed the presence of eighteen novel antimicrobial compounds belonging to four major categories- alcohols, alkaloid, esters and peptide. Conclusion: The study confirms that bioprospection of underexplored habitats can elaborate novel bio and chemodiversity.

ChemInform Abstract: Biologically Active Metabolites of the Actinobacterium Streptomyces sp. GW 33/1593.

ChemInform ◽  
2009 ◽  
Vol 40 (30) ◽  
Author(s):  
M. P. Sobolevskaya ◽  
V. A. Denisenko ◽  
S. Fotso ◽  
H. Laach ◽  
N. I. Menzorova ◽  
...  
Keyword(s):  
Biologically Active ◽  
Active Metabolites ◽  
Streptomyces Sp ◽  
Biologically Active Metabolites

scholarly journals THE STUDY OF THE DOMINANT GROUPS OF BIOLOGICALLY ACTIVE SUBSTANCES AND BIOELEMENTS IN SOME PLANTS OF THE FAMILY ROSACEAE

2017 ◽  
pp. 145-151
Author(s):  
Ефим (Efim) Авраамович (Avraamovich) Краснов (Krasnov) ◽  
Елена (Elena) Евгеньевна (Evgen'evna) Савельева (Savelyeva) ◽  
Надежда (Nadezhda) Кирилловна (Kirillovna) Рыжакова (Ryzhakova) ◽  
Ярослав (Yaroslav) Евгеньевич (Evgen'evich) Решетов (Reshetov) ◽  
Альбина (Al'bina) Равильевна (Ravil'evna) Гатауллина (Gataullina)
Keyword(s):  
Atomic Emission ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Isolation And Identification ◽  
Leading Role ◽  
Aerial Parts ◽  
Potentilla Anserina ◽  
Rosaceae Family ◽  
Innovative Drugs ◽  
Active Substances

To determine the content of the dominant groups of biologically active substances (tannins, polysaccharides, flavonoids) and bioelements in aerial parts of ten species of the Rosaceae family, which grows in Siberia: Potentilla anserina L., P. longifolia Willd., P. canescens Besser, P. bifurca L., P. tergemina Sojak, P. argentea L., P. goldbachii Rupr., P. sericea Dulac., Agrimonia pilosa Ledeb., Filipendula ulmaria (L.) Maxim. To determine the concentrations of essential and conditionally essential trace elements defined by the method of atomic emission spectroscopy. First identified plants of the genus Potentilla (P. canescens, P. tergemina) and Filipendula (F. ulmaria), accumulate significant quantities of lithium – 14,6, and 11,0 13,3 mg/kg, respectively, making them promising for further studies on the isolation and identification of active substances with the aim of creating innovative drugs. Investigated the content of heavy metals (Pb, Hg, As, Cd), the values of which meets regulatory requirements. Useful properties of the examined species family Rosaceae are determined by the content of biologically active substances (flavonoids, polysaccharides, tannins) and the complex of macro - and micronutrients. It is revealed that a dominant position in the spectrum belongs to the macronutrients potassium, calcium and magnesium (4,300 to 27,000 mg/kg). Among micronutrients the leading role is copper, followed by iron, silicon and manganese having important biological value, in particular, involved in the process of hematopoiesis.

scholarly journals Streptomyces sp.: Characterization, Identification and Its Potential as a Ralstonia solanacearum Biological Control Agent in vitro

2019 ◽  
Vol 2 (3) ◽  
pp. 89-96 ◽  
Author(s):  
Rachmad Saputra ◽  
Triwidodo Arwiyanto ◽  
Arif Wibowo
Keyword(s):  
Biological Control ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Biological Control Agent ◽  
Wilt Disease ◽  
Molecular Characteristics ◽  
Streptomyces Sp ◽  
Bacterial Wilt Disease ◽  
Wide Range

Streptomyces sp. bacteria have the potential to produce antibiotic compounds, which are one of the mechanisms that are widely used in biological control. However, in general, biological control mechanisms also occur through competition, cell wall degradation and induced resistance. This study was aimed to determine the physiological, biochemical and molecular characteristics of two isolates of Streptomyces sp. (S-4 and S16 isolates) isolated from the tomatoes roots, and to find out their ability to control Ralstonia solanacearum, which causes bacterial wilt disease on a wide range of hosts. The results showed both Streptomyces sp. isolates had several different physiological and biochemical characteristics and had a different ability to inhibit R. solanacearum in vitro. Streptomyces sp. S-16 isolate had a high similarity with Streptomyces diastaticus subsp. ardesiacus strain NRRL B-1773T based on the molecular identification results. Further research needs to be done to see the potential inhibition of the two Streptomyces isolates in inhibiting the development of bacterial wilt disease in tomato plants caused by R. solanacearum.

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