METABOLIC STATUS OF THE ORGANISM OF BULLS-PRODUCERS OF DIFFERENT GENOTYPES

2020 ◽  
Author(s):  
Н.В. БОГОЛЮБОВА ◽  
Р.А. РЫКОВ
Keyword(s):  
Blood Serum ◽  
Mineral Metabolism ◽  
The Body ◽  
Holstein Breed ◽  
Blood Biochemical ◽  
Health And Nutrition ◽  
Biochemical Status ◽  
Comparative Aspect ◽  
Holstein Bulls ◽  
Blood Biochemical Indicators

Изучили биохимический статус организма быков-производителей голштинской (n=8) и симментальской (n=8) пород в сравнительном аспекте. Проанализированы пробы сыворотки крови быков-производителей, содержащихся в условиях племпредприятия Московской области. В образцах крови определяли показатели азотистого, углеводно-липидного, минерального обменов, активность ферментов. Содержание общего белка и его фракций, мочевины, а также соотношение альбуминов и глобулинов были примерно на одном уровне независимо от генотипа животных. В крови быков симментальской породы, по сравнению с аналогами голштинской породы, была выше концентрация креатинина на 19,9% (P<0,05), уровень фосфолипидов — на 16%, холестерин — на 46,5% (P<0,05). Концентрация фосфора в сыворотке крови симментальских быков составляла 2,52 мМ/л, что на 30,5% больше, чем у голштинов (P<0,05). Отношение кальция к фосфору в организме симментальских быков равнялось 1,04 против 1,40 ед. в группе голшинских быков. Полученные данные указывают на различия в протекании азотистого, углеводно-липидного и минерального обменов в организме животных мясо-молочного и молочного направлений продуктивности и помогут в разработке эталонных значений биохимических показателей крови для оценки здоровья и уровня питания быков-производителей изучаемых пород. We studied the biochemical status of the organism of Holstein (n=8) and Simmental (n=8) bulls-producers in a comparative aspect. Samples of blood serum of bulls-producers contained in the conditions of the Moscow region were analyzed. Indicators of nitrogen, carbohydrate-lipid, mineral metabolism, and enzyme activity were determined in the blood samples. The content of total protein and its fractions, urea, as well as the ratio of albumins and globulins were approximately at the same level, regardless of the genotype of animals. In the blood of Simmental bulls, compared with analogues of the Holstein breed, the concentration of creatinine was higher by 19.9% (P<0.05), the level of phospholipids — by 16%, and cholesterol — by 46.5% (P<0.05). The concentration of phosphorus in the blood serum of Simmental bulls was 2.52 mmol/l, which is 30.5% higher than in Holsteins bulls (P<0.05). The ratio of calcium to phosphorus in the body of Simmental bulls was 1.04 against 1.40 units. in the Holstein bulls. The obtained data indicate differences in the nitrogen, carbohydrate-lipid and mineral metabolism in the body of animals of meat-and-milk and dairy productivity and will help in the reference values of blood biochemical indicators development for assessing the health and nutrition level of bulls-producers of the studied breeds.

scholarly journals КЛІНІКО–БІОХІМІЧНИЙ СТАТУС ДІЙНИХ КОРІВ ПАФ «НЕФЕДІВСЬКЕ» КАМ’ЯНЕЦЬ–ПОДІЛЬСЬКОГО РАЙОНУ ХМЕЛЬНИЦЬКОЇ ОБЛАСТІ

2016 ◽  
Vol 18 (3(71)) ◽  
pp. 37-40
Author(s):  
О.M. Koltun ◽  
V.I. Rusyn
Keyword(s):  
Dairy Cows ◽  
Mineral Metabolism ◽  
Bovine Milk ◽  
Hypochromic Anemia ◽  
Inorganic Phosphorus ◽  
The Body ◽  
Black And White ◽  
Lactating Cows ◽  
Biochemical Status ◽  
Daily Milk Yield

These results of clinical and biochemical status of the herd of dairy cows PAF «Nefedivske» Kamenets–Podolsk district of Khmelnitsky region. The aim was to determine the clinical and biochemical status of the herd of dairy cows and its results to develop measures correcting the violations. Work performed at the PAF «Nefedivske» Kamenets–Podolsk district of Khmelnitsky region. The object of the research were lactating cows, black and white breeds, ages 4 – 6 years and daily milk yield 16 – 18 liters of milk.Survey livestock lactating cows clinically infected animals, which noted the following symptoms: drowsiness animals, reducing distortion and appetite, dullness and disheveled hair cover; delay molting; decrease skin resilience, dry it and thickening; anemic visible mucous membranes; reducing the number of periods and bovine milk production. Serum lactating cows found high content of inorganic phosphorus and iron, and low content of copper and zinc, which constitutes a violation of mineral metabolism. Patient’s hemocytopoesis animals found violations that characterized olihochromemia, decreased hematocrit values and average concentrations of hemoglobin, indicating the development of hypochromic anemia. According to the results of biochemical blood tests in lactating cows installed hipoproteinemia, hipoalbuminemia, and high activity of transaminases and alkaline phosphatase, indicating a violation of metabolic processes in the body sick animals. These results indicate the need for the development and management of correcting the violations of metabolism in dairy cows.

scholarly journals Clinical-biochemical status of hens due to changes of battery cages height location

2021 ◽  
Vol 9 (3) ◽  
pp. 130-134
Author(s):  
M. I. Sakhatsky ◽  
Yu. V. Osadcha
Keyword(s):  
Blood Serum ◽  
Chronic Stress ◽  
Biochemical Parameters ◽  
Physiological State ◽  
Serum Enzymes ◽  
The Body ◽  
Gamma Glutamyl Transferase ◽  
Biochemical Status ◽  
Battery Cages ◽  
Glutamyl Transferase

In the conditions of industrial poultry farming, the body of hens is constantly exposed to numerous technological stressors, the least studied of which are long-term ones, that can provoke chronic stress. One such technological stressor is an increase in the level of cage equipment, which is used by producers to obtain a larger amount of products from 1 m2 of poultry area. The aim of this work was to study the physiological state of the hen’s body based on the clinical biochemical analysis of their blood serum, depending on the battery cages height. In the conditions of a modern complex for the production of edible eggs in a poultry house with an area of 2 915 m2, 4 groups of hens were formed, each of them was kept on a separate floor-analogue in terms of area and cage equipment. Each floor was equipped with «Big Dutchman» 3-tier battery cages. Battery cages of each floor were separated from each other by a lattice floor so that cages of 1–3 tiers entered up to the 1st floor, 4–6 tiers – up to the 2nd, 7–9 tiers – up to the 3rd, and 10–12 tiers – up to 4th floor of cage equipment. Biochemical parameters and activity of hens blood serum enzymes were determined on a BioChem FC-360 biochemical analyzer. For this, 30 blood samples with a volume of 1.0–1.5 ml were taken from axillary veins of laying hens in each group at the age of 18 weeks (at the beginning of the study) and at 52 weeks. It was found that increasing the layering of cage equipment does not have a negative impact on the hen’s body. So, for keeping hens in cages of multi-tier battery cages located on floors 2–4 (4–12 tiers), the biochemical status and activity of their blood serum enzymes were within the physiological norm. Whereas, for hens kept in battery cages of the a multi-tiered system on the 1st floor there was an increase in glucose levels of 60.5–71.0%, creatinine – by 9.7–12.3%, phosphorus – 82.6–100.0%, was observed a decrease in calcium to phosphorus ratio 46.7–50.0%, which was confirmed by an increase in the activity of alkaline phosphatase by 22.3–27.0%, as well as an increase in the activity of aspartate aminotransferase by 3.2–13.8%, lactate dehydrogenase - by 48.5–65.1% and gamma-glutamyl transferase – by 16.4–20.6%. It has been proven that the main consequences of chronic stress caused by keeping hens in the lower floor cells of the multi-tiered battery cage are reflected in the biochemical parameters of their serum, namely increased glucose, creatinine, enzyme activity and violation of calcium to phosphorus ratio.

scholarly journals Influence of the supplement «Press-Acid» on protein-mineral metabolism and resistance of piglets

2018 ◽  
Vol 20 (83) ◽  
pp. 320-324 ◽  
Author(s):  
N.V. Chеrniy ◽  
E.V. Matsenko ◽  
Yu.A. Shchepetilnikov ◽  
Yu. V. Maslak ◽  
O.S. Machula ◽  
...  
Keyword(s):  
Blood Serum ◽  
Mineral Metabolism ◽  
The Body ◽  
Control Group ◽  
Immunological Parameters ◽  
Oxidative Reaction ◽  
Experimental Animals ◽  
Growth Energy ◽  
First Time ◽  
Experimental Group

The aim of this study was to determine the effect of supplement «Press-Acid» on some parameters of mineral and protein metabolism and resistance of piglets. The study was done in condition «Meta» on two groups of piglets of the F-1 (BWхL) genotype. There were determined indexes of bacterial and lysocim activity of blood serum, calcium and phosphorus in piglets at the age of 21–30–45–75 days. Effect of supplement on the growth rate, morbidity and safety of pigs were revealed. We used hygienic, zootechnical, hematological, biochemical, immunological and statistical methods of analysis. The influence of «Press-Acid», which consist of formic, lactic, citric and fumaric acids, was established for the first time. The concentration of calcium in blood serum of experimental group of piglets were lower than in animals of control group. The level of phosphorus increased in blood serum of experimental animals on 11.83% (30 day), on 3.74% (45%), on 17.65% (75 day) respectively. Concentration of hemoglobin and the amount of erythrocytes increased on 11.8% and on 1.82% (30 day) in animals that feed the supplement. There were established the increase of viability of the pigs with gastro-intestinal diseases which were given the supplement in comparison with that, who did not feed it. Severity of diseases was determined using the Melenberg coefficient which did not higher than 1.2–2.4 score, it was lower on 6,0–9,3 unit than in control group. Using the supplement allowed to increase the intensity of growth of piglets by 3.7% (45 day) and 9.4% (75 day) respectively. Concentration of total protein, albumines and globulins increased on 8.66%, 9.90%, 7.3% respectively. The activity of alaninaminotransphaerasae in the blood serum of experimental animals were lower on 3.45% than in control piglets. Lysocym and bacterial activity of blood serum were higher in experimental group of piglets. Feeding piglets with addition supplement Press Acid promotes higher grow rates in experimental animals, that can be explained increases of oxidative-reducing processes in them organism. Feeding piglets with acidifier «Press-Acid», «Nifulin», zinc oxide, probiotics «Lactisan Complex» promotes growth energy, increase of morphological, biochemical and immunological parameters which indicates a higher level of oxidative reaction in the body. A low coefficient of Melenberg is recorded in the piglets at the age of 1 day. Safety was within 92.1–97.0%. That indicates a more successful elimination of complexes of protein, mineral substances, humoral protection in blood of experimental group of animals.

scholarly journals EXPERIMENTAL ENERGY CONCENTRATE "ZEOLFAT" IN THE DIETS OF LACTATING COWS

2020 ◽  
pp. 59-62
Author(s):  
Alia Rinatovna Kashaeva ◽  
Shamil Kasymovich Shakirov ◽  
Firaya Kazbekovna Akhmetzyanova ◽  
Gazimzyan Salimovich Sharafutdinov
Keyword(s):  
Blood Serum ◽  
Economic Efficiency ◽  
Biochemical Parameters ◽  
Mineral Metabolism ◽  
The Body ◽  
Lactating Cows ◽  
Experimental Energy ◽  
Additional Costs ◽  
Positive Effect ◽  
Daily Milk

The introduction of the experimental energy concentrate "Zeolfat" in doses of 200, 400 and 600 g/kg per day into the main diet of lactating cows allowed to increase the content of raw fat by 6.69%, 13.38% and 19.91%; exchange energy by 1.46; 2.92 and 4.37%, respectively. As a result, the increase in average daily milk yields in terms of (3.4%) basic fat content was 8.1% in the I  group with the introduction of 200 g of concentrate, in the II  (400 g) -13.7, in the III  (600 g) - 14.3% compared to the control. A positive effect on the biochemical parameters of blood serum characterizing carbohydrate-lipid and mineral metabolism in the body was established. Economic efficiency per 1 RUB of additional costs in the experimental groups was 2.83-5.53 RUB, respectively.

scholarly journals Вплив препарату «Трифузол» на функціональний стан печінки у курчат за еймеріозної інвазії

2017 ◽  
Vol 19 (77) ◽  
pp. 76-79
Author(s):  
M. Kryshtalska ◽  
V. Hunchak ◽  
B. Gutyj
Keyword(s):  
Liver Function ◽  
Blood Serum ◽  
Total Protein ◽  
Physiological Role ◽  
The Body ◽  
Blood Proteins ◽  
Physiological Variables ◽  
Blood Biochemical ◽  
Vital Functions ◽  
The Impact

In the process of vital functions chicken blood proteins play an important physiological role in the complex processes of metabolism. Changes in blood biochemical composition of chickens according to experimental invasions reflect the development of pathological and immune processes and point on the stress of the body, which makes it possible objectively evaluate the impact of various factors on the organism of infested chickens. We have found that chickens infected eymeriozic invasion in blood serum was a low level of total protein and especially its albumin fraction. This indicates inhibition of protein synthesized liver function. In return the content of globulin in blood serum of sick chickens was 12% higher. On the background of the general hypoproteinemia was found a significant disparity between albumin and globulins in blood serum of sick chickens, as indicated by a decrease in the A/T ratio. After applying for the treatment of chickens affected eymeriozic invasion, drug «Brovafom», due to its antiprotozoic action the influence of toxins eliminates on the liver and intestinal mucosa. However, the protein synthesized function of liver at 28 day of the experiment in experimental chickens has not been completely restored. In applying the drug «Trifuzol» to chickens from the research group D3, for eymeriozic invasion we have found the level increase in total protein and albumin fractions, and also level lowering of globulin in serum of chickens throughout the experiment. At the 21st and 28th day of research in chickens from the experimental group we observe the indices normalization of protein synthesized function of the liver. Within physiological variables was also A/T ratio. Combined use of Brovafom and Trifuzol to experimental chickens for eymeriozic invasion, contributed to better normalization in protein synthesized of liver function, than the use of Brovafom.

scholarly journals Incidence risk of bronchopneumonia in newborn calves associated with intrauterine diselementosis

2020 ◽  
Vol 13 (5) ◽  
pp. 987-995
Author(s):  
Elena Kalaeva ◽  
Vladislav Kalaev ◽  
Anton Chernitskiy ◽  
Mohammad Alhamed ◽  
Vladimir Safonov
Keyword(s):  
Blood Serum ◽  
Reference Range ◽  
Chemical Elements ◽  
Mineral Metabolism ◽  
The Body ◽  
Natural Resistance ◽  
Biochemical Processes ◽  
Incidence Risk ◽  
Newborn Calves ◽  
Newborn Animals

Background and Aim: Macro- and micro-elements are required to ensure the normal course of biochemical processes in the development of an animal's body. Any excess, deficiency, or imbalance in chemical elements in an animal's body can cause the development of various latent or clinically expressed pathological conditions. Diselementosis in pregnant cows may lead to impaired embryo and fetal development, as well as reduced neonatal viability. The aim of this study was to analyze the content of macroelements and microelements in the blood serum of both calving cows and their calves to evaluate the relationship between indicators of mineral metabolism in the mother and newborn and to establish what role separate chemical elements play in making newborn calves more prone to bronchopneumonia. Materials and Methods: The content of potassium (K), sodium (Na), iron (Fe), Copper (Cu), Zinc (Zn), Strontium (Sr), arsenic (As), nickel (Ni), cobalt (Co), chromium (Cr), molybdenum (Mo), and selenium (Se) in the blood serum of 33 pregnant cows between 239 and 262 days of gestation and their 33 1-day old calves was determined using the Shimadzu AA6300 (Japan) atomic adsorption spectrophotometer. Calcium (Ca) and magnesium (Mg) content was determined using ion-selective electrodes from the Olympus-400 analyzer (Beckman Coulter, USA). During the 1st month of life, all calves in the sample set had some sort of respiratory diseases and seven of the calves had bronchopneumonia. Retrospectively, the samples of adult and newborn animals were divided into two groups each: Dams I – cows whose calves had uncomplicated bronchitis (n=26); Dams II – cows whose calves got bronchopneumonia (n=7); and Newborns I – calves with uncomplicated bronchitis (n=26); Newborns II – calves with bronchopneumonia (n=7). Results: The content of Ca, Mg, K, Na, Mo, and Se in dams in both groups of cows was within the reference range; the concentrations of Fe and Ni were higher than the reference range; and the concentrations of Cu, Zn, As, Co, and Cr were lower than the reference range. There were no significant differences in elemental status between the Dams I and Dams II groups. In newborn calves, the concentration of Ca and Mo corresponded to the reference range; the concentrations of Mg, Fe, Co, and Ni in both groups exceeded the reference range; and the concentrations of Cu, Zn, As, Cr, and Se were lower than the reference range. Results highlighted that there was a tendency to decrease concentration of Fe, Mo, and Se and a significant increase in the Ni concentration in calves of the Newborns II group compared with calves of the Newborns I group. It was also found that Zn, Co, Cr, and Mo actively accumulated in the body of newborn animals while the transplacental transfer of Cu, As, and Sr was limited; and transfer of Se and Ni was regulated by concentration ratios in the blood of the mother and the fetus. The excessive concentrations of Ni and Fe in the blood serum of cows and calves and the imbalance in the ratio of elements Fe–Cu–Zn, Fe–Cu–Co negatively affected erythropoiesis, formation of the immune system, and antioxidant status of the fetus and newborn. These changes were considered to be risk factors for the development of bronchopneumonia in calves. Conclusion: An excess of serum Fe and Ni and deficiency of Cu, Zn, As, Co, and Cr in cows during the gestation period can lead to similar impairments of the mineral status in newborn calves. At the systemic level, dyslementosis in combination with the influence of other adverse factors, can lead to an increased load on the respiratory and hematopoietic systems of calves during postnatal adaptation and can subsequently cause a decrease in the natural resistance of calves and development of bronchopneumonia.

scholarly journals Study of the resistance of cows with mastitis depending on the age of lactation

2022 ◽  
Vol 354 (11-12) ◽  
pp. 27-31
Author(s):  
M. V. Dovydenkova
Keyword(s):  
Blood Serum ◽  
Immune Status ◽  
Animal Husbandry ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
The Body ◽  
Nonspecific Resistance ◽  
Animal Blood ◽  
Black And White ◽  
Holstein Breed

Relevance. The breeding stock and young cattle in the conditions of industrial technology of animal husbandry experience an increased antigenic load, which leads to disturbances in the immune status and the development of inflammatory processes manifested by endometritis and mastitis, diarrheal and respiratory syndromes. It was relevant to conduct a comprehensive assessment of the immunological reactivity of the animal’s body according to the state of resistance in cattle with clinical and subclinical mastitis, depending on the age of lactation. As a result of complex studies, the specific and nonspecific reactivity of the body of cattle in different periods of lactation was studied.Methods. The object of the study were cows of a black-and-white Holstein breed (n = 450). Groups of animals were formed according to the age of lactation:1st lactation, 2–3rd lactation, 3–4th lactation and for health reasons: clinically healthy animals, with subclinical mastitis and clinical mastitis. Milk samples were taken from cows to count the number of somatic cells, and blood to determine the quantitative content of IgG immunoglobulin and indicators of nonspecific resistance.Results. It was found that with mastitis in cows of different lactation ages, almost identical changes in the immune status were observed. Quantitative determination of IgG levels in animal blood serum is the basis for assessing the immunity of cattle. The immune status in clinically healthy animals was characterized by a higher level of the total amount of IgG immunoglobulin in the blood serum (amounted to 22.25 ± 0.70 mg/ml), whereas in cows with a subclinical form of mastitis, the level of IgG immunoglobulin decreased by 12.3%, with a clinical form of mastitis — by 17.2%. The results also showed that in cows of different lactations with subclinical and clinical mastitis, the bactericidal activity (by 10.8%) and the lysozyme activity of the blood increased significantly (by 8.22%). In cows with clinical mastitis — by 8.6% and 9.7% respectively, compared with the indicators in groups of healthy animals. Thus, mastitis, occurring in acute and subacute form, leads to a decrease in the resistance of the cows’ organism.

scholarly journals Study On Laying Performances, Blood Biochemical Indicators, Feather Condition And Antibody Titers of Layer Breeders Among Different Tiers In Mating Cages During The Peak Laying Period*

2021 ◽  
Author(s):  
Yan LI ◽  
Rui-yu MA ◽  
Yi WAN ◽  
Renrong QI ◽  
Jun-ying LI ◽  
...  
Keyword(s):  
Egg Quality ◽  
Shape Index ◽  
The Body ◽  
Average Score ◽  
Shell Weight ◽  
Biochemical Indicators ◽  
Blood Biochemical ◽  
Antibody Titers ◽  
Parent Stock ◽  
Blood Biochemical Indicators

Abstract The present study was conducted to determine the effect of cage tier on laying performances, blood biochemical indicators, feather condition and antibody titers of layer breeders among different tiers in mating cages during the peak laying period. All birds (Hy-Line Brown parent stock breeders) at 27 weeks of age were housed in 4-tier mating cage. Laying performances, egg quality characteristics, blood biochemical indicators, antibody titers and feather condition were measured. The highest laying rate was recorded at 2nd tier, followed by 4th tier and 3rd tier, and the lowest at 1st tier. There were no significant differences among different tiers in terms of percentage of defective eggs and hatchability performances. Yolk color was significantly lower at 2nd tier than at other tiers. However, no distinct differences in egg weight, shell weight, shell color, shell breaking strength, egg shape index, yolk weight, albumen height, haugh units were observed. Concentrations of serum CK, T-AOC, SOD, GPx and MDA, and the antibody titers did not change substantially among different tiers. Moreover, the average feather score for each individual part of the body and an overall average score for birds were not significantly different. We confidently conclude that cage tier may be a contributing factor for layer performances. These findings will be helpful for application of natural mating system in layer industry.

scholarly journals Blood biochemical parameters in transfer factor donor cows depending on sensitization scheme

2020 ◽  
Vol 11 (4) ◽  
Author(s):  
V. G. Skybitskyi ◽  
◽  
V. V. Postoi ◽  
H. V. Kozlovska ◽  
F. Zh. Ibatullina ◽  
...  
Keyword(s):  
Blood Serum ◽  
Transfer Factor ◽  
Biochemical Parameters ◽  
The Body ◽  
Aminotransferase Activity ◽  
Vaccine Administration ◽  
Blood Biochemical Parameters ◽  
Blood Biochemical ◽  
Experimental Group ◽  
Donor Cows

Research and development of means for effective prevention and treatment of diseases in animals are one of the priorities for modern veterinary science. Means based on the transfer factor are quite promising to solve these problems. One of the stages of obtaining a qualitative transfer factor specific to a particular disease is the sensitization of the body of donor animals. The purpose of this work was to investigate the blood biochemical parameters of donor cows after sensitization according to different schemes. The experiments were performed on cows of the Ukrainian black-spotted dairy breed, aged 4–5 years. Sensitization of pregnant cows was performed 1–1.5 months before calving with a concentrated formol-alum vaccine against salmonellosis of calves manufactured by the Kherson Biofactory. The vaccine was administered to the animals of the first experimental group one month before calving, one-time in a dose of 10 ml. Animals of the second experimental group 1.5 months before calving were two-time vaccine administered with an interval between injections of 10 days in doses of 10 and 15 ml. Studies have shown that in donor cows, which were two-time vaccine administered, there was an increase in hemoglobin content by 13% (P<0.05). There was also a decrease in glucose and creatinine content by 13–28% (P<0.05–0.01) in the blood serum of pregnant cows, which did not depend on the sensitization scheme, and a tendency to a decrease in total protein content. Regardless of the sensitization scheme of cows, an increase in serum aminotransferase activity was observed by 1.3–1.5 times (P<0.05–0.001), and if alanine aminotransferase activity increased mainly with a single injection of the vaccine, then aspartate aminotransferase activity was more intensively increased after a two-time vaccine administration. There was a slight decrease in сalcium (by 5–9%) and phosphorus (by 2–3%) content and an increase in potassium content (by 2–5%) in the blood serum of pregnant cows two weeks after vaccine administration regardless of the sensitization scheme.

Effect of pig housing system on blood biochemical indicators’ level

2018 ◽  
Vol 35 (4) ◽  
pp. 45-51
Author(s):  
Maria Tietze ◽  
Wanda Krupa ◽  
Monika Budzyńska ◽  
Jarosław Kamieniak ◽  
Leszek Sołtys ◽  
...  
Keyword(s):  
Housing System ◽  
Biochemical Indicators ◽  
Blood Biochemical ◽  
Blood Biochemical Indicators
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