scholarly journals Primary evaluation of newly isolated strains of phosphate-mobilizing bacteria

2021 ◽  
Vol 1 (25) ◽  
pp. 28-37
Author(s):  
M.I. Baranskaya ◽  
◽  
L.A. Chaikovskaya ◽  
Keyword(s):  
Winter Wheat ◽  
Germination Rate ◽  
Colorimetric Method ◽  
Culture Fluid ◽  
Soluble Form ◽  
Bacterial Strains ◽  
Effective Microorganisms ◽  
Primary Evaluation ◽  
University Laboratory ◽  
Wheat Seeds

Isolation and screening of strains with increased phosphate-dissolving activity are necessary stages of research on the intensification of phosphorus nutrition of plants by pre-sowing inoculation of seeds with effective microorganisms. The purpose of the research was to study the phosphate-dissolving activity of newly isolated bacterial strains and their effect on the sowing qualities of winter wheat seeds. Phosphate-mobilizing bacteria were isolated in 2014–2019 from the сhernozem southern sampled in the fields of the Order of the Red Banner of Labour Agro-Industrial College – structural unit of V.I. Vernadsky Crimean Federal University. Laboratory studies were carried out in 2019–2020 in the Department of Agricultural Microbiology of the Research Institute of Agriculture of Crimea. The isolation of strains of soil phosphate-mobilizing bacteria was carried out based on the appearance of a clear zone around the colonies on agarized glucose-aspartic (GA) medium supplemented with Ca3(PO4)2. The coefficient of specific phosphate-dissolving activity (Kr) of the strains was calculated according to the method of I. M. Malinovskaya. The quantitative assessment of the phosphate-dissolving activity of bacteria in a liquid medium (P2O5 content) was determined by the colorimetric method. High Kr is typical for the predominant part of the studied strains (22 or 81.5 %). The highest Kr value (10) was noted for strain 0613. Twelve (44 %) strains demonstrated the highest ability to transform Ca3(PO4)2 into a soluble form. The maximum content of P2O5 (212 mg/l) was observed in the culture fluid of strain 1702. When winter wheat seeds were inoculated with strains 0890 and 1702, germination increased by 8–9 %. The same was with germination energy; it also increased compared to control (water) by 2.5 and 7.5 %, respectively. The use of strain 1303 contributed to a significant increase in the energy and germination rate of seeds: by 34.5, 6 and 5.6 %, respectively, compared to control. The strains provided a significant increase in the weight of seedlings compared to control: by 29 % – the reference strain; by 32, 38 and 29 % – newly isolated 0735, 1204, 1701, respectively.

scholarly journals BACTERIA OF THE ORDER BACILLALES AS PROMISING ANTAGONISTS OF FUSARIUM PATHOGENS AND THEIR IMPACT ON WINTER WHEAT PLANTS

2020 ◽  
Author(s):  
N.G. Vasilchenko ◽  
◽  
A.V. Gorovtsov ◽  
V.A. Chistyakov ◽  
M.S. Mazanko
Keyword(s):  
Winter Wheat ◽  
Phytopathogenic Fungi ◽  
Soil Samples ◽  
Morphometric Parameters ◽  
Bacterial Strains ◽  
Soil Suspension ◽  
Wort Agar ◽  
Genus Fusarium ◽  
Fusarium Pathogens ◽  
Wheat Seeds

The possibility of using bacteria of the order Bacillales as agents of biological control of phytopathogenic fungi of the genus Fusarium was studied. In the work, 28 soil samples were studied, from which antagonist bacterial strains were isolated. Antagonism was detected by cultivating a pasteurized soil suspension with a culture of the fungus Fusarium graminearum on wort agar. In the course of this work, 1040 antagonist bacterial strains were isolated. Subsequently, the influence of the selected strains of microorganisms on the germination winter wheat seeds and several other morphometric parameters was studied.

scholarly journals Improving the efficiency of pre-sowing treatment of winter wheat seeds with low power coherent optical radiation

2021 ◽  
Vol 659 (1) ◽  
pp. 012019
Author(s):  
M A Taranov ◽  
A S Kazakova ◽  
P V Gulyaev ◽  
M M Ukraintsev ◽  
A S Tatarintsev
Keyword(s):  
Winter Wheat ◽  
Low Power ◽  
Optical Radiation ◽  
Wheat Seeds

Aktywność przeciwutleniająca i przeciwbakteryjna miodu, propolisu oraz pyłku kwiatowego

2020 ◽  
Vol 21 (3) ◽  
Author(s):  
Magdalena Woźniak ◽  
Lucyna Mrówczyńska ◽  
Anna Sip ◽  
Marta Babicka ◽  
Tomasz Rogoziński ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Pathogenic Bacteria ◽  
Radical Scavenging Activity ◽  
Colorimetric Method ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Content ◽  
Diffusion Method ◽  
Bacterial Strains

Introduction. Honey, propolis and pollen belong to bee products that have beneficial biological properties. These products exhibit e.g. antibacterial, antifungal and antioxidant properties. Due to biological activity and natural origin, bee products are used, e.g. in the food industry, cosmetology and pharmacy. Aim. The aim of the study was to compare the antioxidant and antibacterial activity of honey, propolis and pollen from an apiary located in Wielkopolska Province. Material and methods. Honey, propolis and pollen used in this study came from the same apiary located in Wielkopolska Province. The antioxidant potential of bee products was evaluated applying DPPH· free radical scavenging activity assay. The antimicrobial activity of the tested bee products was determined by the point-diffusion method against 13 strains of pathogenic and potentially pathogenic bacteria. In addition, the total content of phenolic compounds in honey, propolis and pollen was determined by the colorimetric method. Results. Propolis exhibited higher antioxidant activity, in comparison to honey and pollen. The antiradical activity of propolis was equal to 80% approx. activity of Trolox, the standard antioxidant. Among tested bee products, propolis was characterized by the highest total phenols content. In addition, honey, propolis and pollen showed antagonistic activity against tested bacterial strains. Conclusions. The obtained results indicate that among the tested bee products of native origin, i.e. honey, propolis and pollen, propolis characterized by the highest antioxidant activity and the total content of phenolic compounds. In addition, all bee products showed bactericidal activity against the tested bacterial strains.

THE EFFECT OF MANGANESE AND MOLYBDENUM FERTILIZERS ON THE SOWING QUALITIES OF WINTER WHEAT SEEDS

2021 ◽  
pp. 80-92
Author(s):  
Lyudmila Mikhailovna Onishchenko ◽  
Lyubov Vladimirovna Streletskaya ◽  
Daniil Sergeevich Karikov
Keyword(s):  
Winter Wheat ◽  
Wheat Seeds

scholarly journals Selecting Wheat Seeds of Moderate Phytate Using Colorimetric Method

2011 ◽  
Vol 3 (2) ◽  
Author(s):  
Xinglin Li ◽  
Dandan Wang ◽  
Jie Gao ◽  
Xinyan Li ◽  
Xinghua Cui ◽  
...  
Keyword(s):  
Colorimetric Method ◽  
Wheat Seeds

scholarly journals EFFECT OF BACILLUS GINSENGIHUMI M2.11 AND PANTOEA BRENNERI AS3 ON PLANT BIOMASS GROWTH AND SEED GERMINATION ENERGY

ÈKOBIOTEH ◽  
2021 ◽  
Vol 4 (1) ◽  
pp. 49-55
Author(s):  
D.L. Itkina ◽  
◽  
L.V. Sokolnikova ◽  
A.D. Suleimanova ◽  
M.R. Sharipova ◽  
...  
Keyword(s):  
Agricultural Development ◽  
Plant Biomass ◽  
Plant Protection ◽  
Average Length ◽  
Culture Fluid ◽  
Plant Protection Products ◽  
Practical Significance ◽  
Mineral Fertilizers ◽  
Bacterial Strains ◽  
Active Metabolites

In the modern conditions of agricultural development, the use of microbiological preparations as an alternative to mineral fertilizers and chemical plant protection products is gaining wide interest and practical significance. Soil microorganisms have the ability to interact with the root system of plants, optimize mineral nutrition, synthesize growth hormones and antimicrobial compounds that inhibit the development of phytopathogenic fungi and bacteria and have a stimulating effect on the growth and development of cultivated crops, increasing their resistance to phytopathogens and stress. The search for environmentally friendly technologies, the use of enzymes and active metabolites of bacterial origin, or the use of bacterial strains that promote plant growth is a promising direction. The effect of culture fluid of Bacillus ginsengihumi and Pantoea brenneri on the average length of the plant stem was studied. When processing wheat seeds of P. brenner AS3, the length of the first leaf increased by 50%, and B. ginsengihumi M2.11 by 25%.

scholarly journals Determination of Main Spectral and Luminescent Characteristics of Winter Wheat Seeds Infected with Pathogenic Microflora

Photonics ◽  
2021 ◽  
Vol 8 (11) ◽  
pp. 494
Author(s):  
Alexey M. Bashilov ◽  
Igor Yu. Efremenkov ◽  
Mikhail V. Belyakov ◽  
Alexander V. Lavrov ◽  
Anatoly A. Gulyaev ◽  
...  
Keyword(s):  
Winter Wheat ◽  
Physical Properties ◽  
Agricultural Products ◽  
High Quality ◽  
Crop Losses ◽  
Software Complex ◽  
Fusarium Infection ◽  
Constant Growth ◽  
Wheat Seeds

In connection with the constant growth of demand for high-quality food products, there is a need to develop effective methods for storing agricultural products, and the registration and predicting infection in the early stages. The studying of the physical properties of infected plants and seeds has fundamental importance for determining crop losses, conducting a survey of diseases, and assessing the effectiveness of their control (assessment of the resistance of crops and varieties, the effect of fungicides, etc.). Presently, photoluminescent methods for diagnosing seeds in the ultraviolet and visible ranges have not been studied. For research, seeds of winter wheat were selected, and were infected with one of the most common and dangerous diseases for plants—fusarium. The research of luminescence was carried out based on a hardware–software complex consisting of a multifunctional spectrofluorometer “Fluorat-02-Panorama”, a computer with software “Panorama Pro” installed, and an external camera for the samples under study. Spectra were obtained with a diagnostic range of winter wheat seeds of 220–400 nm. Based on the results obtained for winter wheat seeds, it is possible to further develop a method for determining the degree of fusarium infection.

scholarly journals Dengue Virus Type 1 Nonstructural Glycoprotein NS1 Is Secreted from Mammalian Cells as a Soluble Hexamer in a Glycosylation-Dependent Fashion

1999 ◽  
Vol 73 (7) ◽  
pp. 6104-6110 ◽  
Author(s):  
Marie Flamand ◽  
Françoise Megret ◽  
Magali Mathieu ◽  
Jean Lepault ◽  
Félix A. Rey ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Virus Type ◽  
Mammalian Cells ◽  
Culture Fluid ◽  
Vero Cells ◽  
Soluble Form ◽  
Complex Type ◽  
Infected Insect ◽  
Infected Cells

ABSTRACT Nonstructural glycoprotein NS1, specified by dengue virus type 1 (Den-1), is secreted from infected green monkey kidney (Vero) cells in a major soluble form characterized by biochemical and biophysical means as a unique hexameric species. This noncovalently bound oligomer is formed by three dimeric subunits and has a molecular mass of 310 kDa and a Stokes radius of 64.4 Å. During protein export, one of the two oligosaccharides of NS1 is processed into an endo-β-N-acetylglucosaminidase F-resistant complex-type sugar while the other remains of the polymannose type, protected in the dimeric subunit from the action of maturation enzymes. Complete processing of the complex-type sugar appears to be required for efficient release of soluble NS1 into the culture fluid of infected cells, as suggested by the repressive effects of the N-glycan processing inhibitors swainsonine and deoxymannojyrimicin. These results, together with observations related to the absence of secretion of NS1 from Den-infected insect cells, suggest that maturation and secretion of hexameric NS1 depend on the glycosylation status of the host cell.

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