scholarly journals Chemical Constituents of Eremostachys macrophylla Montbr. & Auch. Aerial Parts

2020 ◽  
Vol 26 (2) ◽  
pp. 203-208
Author(s):  
Parina Asgharian ◽  
Abbas Delazar ◽  
Solmaz Asnaashari
Keyword(s):  
High Performance ◽  
Chemical Constituents ◽  
Folk Medicine ◽  
Reversed Phase ◽  
Spectroscopic Technique ◽  
Phytochemical Analysis ◽  
Aerial Parts ◽  
Soxhlet Apparatus ◽  
Snake Bites ◽  
Pure Compounds

Background: Eremostachys macrophylla Montbr. & Auch. is one of the wild growing species of herbs found in East Azerbaijan province of Iran. These species are used in folk medicine for the healing of wound, treatment of snake bites, rheumatism and joint pains. The primary aim of this study was to obtain natural pure compounds and this was done by subjecting the aerial parts of Eremostachys macrophylla Montbr. & Auch. to phytochemical analysis. Methods: The air-dried and crushed aerial parts were respectively extracted with n-hexane, dichloromethane (DCM) and methanol (MeOH) solvents using a soxhlet apparatus. The 10%, 20% and 40% of MeOH in water Sep-Pak fractions of the MeOH extract were subjected to a preparative reversed- phase high performance liquid chromatography (RP-HPLC). Also, the isolated pure compounds were identified by one-dimensional nuclear magnetic resonance (1D-NMR) spectroscopic technique. Results: The results obtained in this study showed the presence of seven pure components; (1) Lamalbide, (2) Sesamoside, (3) Phlomiol, (4) Verbascoside, (5) Luteolin-7-O- glucoside, (6) Apigenin-7-O- rutinoside and (7) Kaempferol-3-O- glucoside with iridoid, phenylethanoid and flavonoid structures. Conclusion: The results from the study demonstrated that the aerial parts of E. macrophylla could be a good source of iridoids, phenylethanoids and flavonoids.

scholarly journals Phytochemical Investigation of Aerial Parts of Iraqi Cardaria draba

2020 ◽  
Vol 29 (2) ◽  
pp. 27-36
Author(s):  
Alaa M. Abd ◽  
Enas J. Kadhim
Keyword(s):  
High Performance Liquid Chromatography ◽  
Phenolic Acid ◽  
High Performance ◽  
Chemical Constituents ◽  
Aqueous Methanol ◽  
Plant Materials ◽  
Aerial Parts ◽  
Phytochemical Investigation ◽  
Soxhlet Apparatus ◽  
Layer Chromatography

 The aim of this study was to study chemical constituents of aerial parts of Cardaria draba since no phytochemical investigation had been studied before in Iraq. Aerial parts of Cardaria draba were defatted by maceration in hexane for 72 h. The defatted plant materials were extracted using Soxhlet apparatus, the aqueous Methanol 90% as a solvent extraction for 18 h, and fractionated with petroleum ether- chloroform (CHCl3)- ethylacetate- and n-butanol respectivly. The ethyl acetate, n-butanol, and n-butanol after hydrolysis fractions were investigated by high performance liquid chromatography (HPLC) and thin-layer chromatography (TLC) for its phenolic acid and flavonoid contents. Flavonoids and phenolic acid derivative were isolated from the ethylacetate of leaf fraction and n-butanol after hydrolysis fraction of the aerial parts and identified by TLC, FTIR and HPLC. A various chromatographic and spectroscopic results shown the presence of luteolin, chlorogenic acid, caffeic acid, and resorcinol in aerial parts of C. draba.                                                                                                                              

scholarly journals Brazilian Red Propolis—Chemical Composition and Botanical Origin

2008 ◽  
Vol 5 (4) ◽  
pp. 435-441 ◽  
Author(s):  
Andreas Daugsch ◽  
Cleber S. Moraes ◽  
Patricia Fort ◽  
Yong K. Park
Keyword(s):  
High Performance ◽  
Chemical Constituents ◽  
Scientific Information ◽  
Folk Medicine ◽  
Reversed Phase ◽  
Northeastern Brazil ◽  
Botanical Origin ◽  
Woody Perennial ◽  
Layer Chromatography ◽  
Plant Sources

Propolis contains resinous substances collected by honey bees from various plant sources and has been used as a traditional folk medicine since ca 300 BC. Nowadays, the use of evidence-based complementary and alternative medicine (CAM) is increasing rapidly and so is the use of propolis in order to treat or support the treatment of various diseases. Much attention has been focused on propolis fromPopulussp. (Salicaceae) andBaccharis dracunculifolia(Asteracea), but scientific information about the numerous other types of propolis is still sparse. We gathered six samples of red propolis in five states of Northeastern Brazil. The beehives were located near woody perennial shrubs along the sea and river shores. The bees were observed to collect red resinous exudates onDalbergia ecastophyllum (L) Taub. (Leguminosae) to make propolis. The flavonoids of propolis and red resinous exudates were investigated using reversed-phase high-performance liquid chromatography and reversed-phase high-performance thin-layer chromatography. We conclude that the botanical origin of the reddish propolis isD. ecastophyllum. In areas where this source (D. ecastophyllum) was scarce or missing, bees were collecting resinous material from other plants. Propolis, which contained the chemical constituents from the main botanical origin, showed higher antimicrobial activity.

scholarly journals Phytochemical analysis and hypotensive activity of Ipomoea pes-caprae on blood pressure of normotensive rats

Rodriguésia ◽  
2020 ◽  
Vol 71 ◽  
Author(s):  
Fernanda Manhães Braga Gonçalves ◽  
Amaro Chaves Ramos ◽  
Marcelo da Silva Mathias ◽  
Queitilane de Souza Sales ◽  
Carolina Chaves Ramos ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Locomotor Activity ◽  
High Performance ◽  
Folk Medicine ◽  
Ultra Performance Liquid Chromatography ◽  
Hypotensive Activity ◽  
Phytochemical Analysis ◽  
Hydroalcoholic Extract ◽  
Aerial Parts ◽  
Significant Impairment

Abstract Ipomoea pes-caprae (Convolvulaceae), popularly known as “salsa-da-praia”, is used in folk medicine for the treatment of several diseases, including hypertension. The aim of this research was to evaluate hypotensive activity attributed to this species and identify the bioactive compounds responsible for this effect. Hydroalcoholic extract of aerial parts of Ipomoea pes-caprae (PIEA) was fractionated by column chromatography with Amberlite XAD-2, thus achieving a methanolic fraction (PIEA-M). The chemical profile investigation of PIEA-M was conducted by Ultra Performance Liquid Chromatography coupled to Mass Spectrometry (UPLC-MS) and compound isolation through High Performance Countercurrent Chromatography (HPCCC). Hemodynamic evaluations of PIEA, PIEA-M and isolated compounds were carried out on normotensive rats. The locomotor activity of PIEA and PIEA-M were performed by Rota Rod test using Swiss mice. UPLC-MS analysis of PIEA-M led to the identification of 11 compounds corresponding to seven phenolic acids and four flavonoids. By means of HPCCC, quercetin 6”-O-acetyl-3-O- glucoside (1), a mixture of quercetin-3-O-galactoside and quercetin-3-O- glucoside (2) and quercetin 3-O-glucoside (3) were isolated. PIEA, PIEA-M and the obtained compounds (1 and 2) significantly reduced blood pressure. Also, PIEA and PIEA-M caused significant impairment on mice locomotor activity. These results indicate the potential of Ipomoea pes-caprae as a source of hypotensive agents suggesting that phenolic compounds present in the species are responsible for its hypotensive activity.

scholarly journals High Performance Thin Layer Chromatography Fingerprinting Analysis of Piper betle L. Leaves

2021 ◽  
pp. 8-15
Author(s):  
Ramdas N. Kale ◽  
Ravindra Y. Patil
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Methanolic Extract ◽  
Chemical Constituents ◽  
Piper Betle ◽  
Soxhlet Apparatus ◽  
Rf Values ◽  
Pharmacologically Active ◽  
Layer Chromatography

Introduction: Many modern medicines used today based on plants and plant products. Piper betle is generally known as the betle vine, it is an important medicinal and recreational plant. High performance thin layer chromatography (HPTLC) is an advanced powerful analytical method with more separation power, high performance and superior reproducibility than classic thin layer chromatography (TLC). A chromatographic fingerprint of a plant extract is a chromatographic pattern of some common chemical constituents of pharmacologically active and/or chemical characteristics. Chromatographic fingerprints are useful in authentication and identification of plant. Objectives:  Objectives of present research was to establish HPTLC fingerprinting of methanolic extract of Piper betle L. leaves. Materials and Methods: Methanolic extract of Piper betle leaves was prepared using soxhlet apparatus. HPTLC studies were performed using a CAMAG HPTLC system equipped with automatic TLC sampler-4 (ATS 4), TLC scanner 4, and vision CATS 3.0 software. Results: The study revealed the presence of alkaloids with Rf value 0.65, flavonoids with Rf values 0.19, 0.29, 0.72, 0.95., and phenolic compound with Rf value 0.7. Conclusion: The HPTLC fingerprinting profile developed for the methanolic extract of Piper betle L. leaves will help in proper identification of the plant.Piper betle

scholarly journals Antioxidant Activity and Isolation of Luteoline fromCentaurea behenL. Grown in Iran

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Akbar Esmaeili ◽  
Zahra Mousavi ◽  
Maryam Shokrollahi ◽  
Ali Shafaghat
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Biological Activities ◽  
Uv Spectroscopy ◽  
Multiple Bond ◽  
New Drugs ◽  
Terrestrial Plants ◽  
Total Extract ◽  
Aerial Parts ◽  
Soxhlet Apparatus

Flavonoids are secondary metabolites providing Ultraviolet-visible (UV) spectroscopy protection and color in almost all terrestrial plants and fruits. They have a fused ring system consisting of an aromatic ring and a benzopyran ring with a phenyl substituent. As their biological activities have an impact on human health, they serve as target molecules in the development of new drugs. The objective of this research was to study the antioxidant activity and chemical analysis of the luteoline fromCentaurea behenL. (Compositae family). The aerial parts of powdered and driedC. behenwere extracted with methanol (MeOH) in a Soxhlet apparatus over a period of 2 days. The concentrated total extract was extracted with petroleum ether, diethylether, and methanol. From the methanol extract of the aerial parts ofC. behen, the flavonoid derivative (luteoline) was identified. The aerial parts’ extract demonstrated effective antioxidant activity measured in terms of half-maximal inhibitory concentration (IC50). The product extract has been isolated by UV, column chromatography (CC), and preparative high-performance liquid chromatography (HPLC). The structures involved were elucidated by1H and13C nuclear magnetic resonance (NMR) and heteronuclear multiple-bond correlation (HMBC) spectra. The compound identified had not been reported in previous studies ofC. behenL.

scholarly journals CYTOTOXIC CONSTITUENTS FROM AERIAL PARTS OF HELICTERES HIRSUTA PLANT, COLLECTED IN BINH PHUOC PROVINCE

2018 ◽  
Vol 127 (1A) ◽  
pp. 111
Author(s):  
Pham Huu Dien
Keyword(s):  
Hela Cell ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Folk Medicine ◽  
Hep G2 ◽  
Aerial Parts ◽  
Hela Cell Lines ◽  
Human Prostate Carcinoma ◽  
Pure Compounds ◽  
Human Lung Carcinoma

<p><em>Helicteres hirsuta</em> plant is a member of <em>Helicteres </em>genus<em> </em>of the plant family Sterculiaceae and widely found in countries of South Asia such as Vietnam, Lao, Thailand etc. In recent years, it is known as a new folk medicine protecting and securing people against human lung carcinoma, hormone - dependent human prostate carcinoma and human liver. In this paper, from aerial parts of <em>Helicteres hirsuta</em> plant, collected in Binhphuoc province (October, 2016) three pure compounds <strong>1</strong>-<strong>3</strong> were isolated and structurally elucidated, such as <em>b</em>-stigmasterol (<strong>1</strong>), protosta-17(20), 24-dien-3<em>b</em>-ol (<strong>2</strong>) and icosanoic acid (<strong>3</strong>). Among them, compound <strong>2</strong> has a remarkable cytotoxic activity against SK-LU-1, Hep-G2, and Hela cell lines with IC<sub>50</sub> values from 32.86 to 77.31 mg/mL, meanwhile compound <strong>3</strong> shows a moderable cytotoxic activity against SK-Mel-2, AGS, SK-LU-1, Hep-G2, and Hela cell lines with IC<sub>50</sub> values from 59.02 to 80.87 mg/mL.  </p>

Chemical constituents, antimicrobial and antioxidant properties of the aerial parts of Coccinia barteri

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Ethyl Acetate ◽  
Methanol Extract ◽  
Chemical Constituents ◽  
Antioxidant Properties ◽  
Phytochemical Analysis ◽  
Methanol Extracts ◽  
Aerial Parts ◽  
Ethyl Hexadecanoate ◽  
Bacteria And Fungi ◽  
Percentage Abundance

Phytochemical analysis of n-hexane, ethyl acetate and methanol extracts of the aerial parts of Coccinia barteri was carried out. These extracts exhibited satisfactory inhibitory activities against bacteria and fungi strains, which include; Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, Salmonella typhii, Klebsiella pneumoniae, Candida albicans, Aspergillus niger, Penicillium notatum and Rhizopus stolonifer. Methanol extract of C. barteri possesses antioxidant activity by scavenging DPPH free radical with IC50 of 187.56 μg/mL, using DPPH antioxidant assay. GC-MS analysis of n-hexane, ethyl acetate and methanol extracts of the plant principally revealed the presence of phytol, ethyl hexadecanoate and clionasterol with their corresponding percentage abundance of 57.75%, 18.33% and 9.79%, respectively.

scholarly journals Comparative Studies of Fraxinus Species from Korea Using Microscopic Characterization, Phytochemical Analysis, and Anti-Lipase Enzyme Activity

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 534
Author(s):  
Kazi-Marjahan Akter ◽  
Woo Sung Park ◽  
Hye-Jin Kim ◽  
Atif Ali Khan Khalil ◽  
Mi-Jeong Ahn
Keyword(s):  
Lipase Activity ◽  
High Performance ◽  
Folk Medicine ◽  
Lower Surface ◽  
Stem Bark ◽  
Phytochemical Analysis ◽  
Orthogonal Projections ◽  
Cork Cell ◽  
Microscopic Evaluation ◽  
Chemical Profiles

Fraxinus species belongs to the Oleaceae family, commonly known as Ash tree, and has been utilized as a folk medicine with various medicinal properties, including anti-obesity activity. The goal of the present study was to establish quality control parameters using microscopic characterization, phytochemical differentiation, and anti-lipase activity evaluation of five Fraxinus plants in Korea. Microscopic evaluation of the lower surface, petiole, and midrib of leaves, and stem bark showed discriminative anatomical characteristics, such as the stomatal index of the lower leaf surface; the number of sclerenchyma cells, and the diameter of parenchyma cells in the petiole and midrib; and the cork cell size and fiber frequency in the stem bark. Phytochemical analysis using high-performance liquid chromatography revealed the significant variation in the chemical profiles of the 12 major secondary metabolites among the samples. The orthogonal projections to latent structure-discrimination analysis efficiently differentiated each group belonging to each Fraxinus plant with the anatomical and quantification data. F. rhynchophylla and ligstroside showed the most potent anti-lipase activity among the plants and the 12 major metabolites, respectively. These findings could serve as the scientific criteria for the appropriate identification and establishment of standards for the use of Fraxinus species as medicinal plants.

scholarly journals Phytochemical Analysis of Phillyrea latifolia L., a New Source of Oleuropeoside

2001 ◽  
Vol 56 (5-6) ◽  
pp. 353-356 ◽  
Author(s):  
Cristina Recuero Carretero ◽  
Ana M. Díaz Lanza ◽  
Lidia Fernández Matellano ◽  
Angel Rumbero Sánchezb ◽  
Lucinda Villaescusa Castillo
Keyword(s):  
Chemical Constituents ◽  
Biologically Active ◽  
Phytochemical Analysis ◽  
Mediterranean Species ◽  
Aerial Parts ◽  
Phillyrea Latifolia ◽  
Triterpenic Acids ◽  
Phenylpropanoid Glycosides ◽  
Secoiridoid Glycosides ◽  
Active Substances

As a part of our studies on the biologically active substances from Spanish plants, we have undertaken an investigation of the chemical constituents of a typical mediterranean species, Phillyrea latifolia L. (Oleaceae). Two secoiridoid glycosides, three phenylpropanoid glycosides, one lignane and two triterpenic acids were isolated from the leaves of this species and identified. The phytochemical analysis of the aerial parts of P. latifolia revealed that it is a rich source of oleuropeoside.

scholarly journals HPLC - DAD Analysis of Lycorine in Amaryllidaceae Species

2010 ◽  
Vol 5 (6) ◽  
pp. 1934578X1000500
Author(s):  
Gulen Irem Kaya ◽  
Derya Cicek ◽  
Buket Sarıkaya ◽  
Mustafa Ali Onur ◽  
Nehir Unver Somer
Keyword(s):  
High Performance ◽  
Biological Activities ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Array Detector ◽  
Simple Method ◽  
Aerial Parts ◽  
Liquid Chromatographic Method ◽  
Low Mass ◽  
Liquid Chromatographic

Lycorine, the most frequent alkaloid found in Amaryllidaceae plants, has been proven to have various biological activities. Therefore, it is important to quantify this compound in Amaryllidacaeae species. In this study, a reversed-phase high performance liquid chromatographic method has been used and validated for the determination of lycorine in Amaryllidaceae plants. A simple method for the extraction of lycorine in low-mass plant samples was employed utilizing columns pre-packed with diatomaceous earth (Extrelut®). This method was applied to the aerial parts and bulbs of Sternbergia sicula Tineo ex Guss., S. lutea (L.) Ker-Gawl. ex Sprengel and Pancratium maritimum L. (Amaryllidaceae) collected during two different vegetation periods. The chromatographic separation was performed using an isocratic system with a mobile phase of trifluoroacetic acid-water-acetonitrile (0.01:90:10) applied at a flow rate 1 mL min−1 using diode array detector. Validation procedures showed that the method was specific, accurate and precise. The amount of lycorine in S. sicula samples ranged between 0.10-0.53%, whereas in those of S. lutea and P. maritimum, the range was 0.19-0.40% and 0.05-0.14%, respectively.

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