ARYLHYDROCARBON RECEPTOR NUCLEAR TRANSLOCATOR () IN HUMAN SKIN DURING AGING

2020 ◽  
pp. 313-318
Author(s):  
А. Г. Гунин ◽  
Н. Н. Голубцова ◽  
Н. К. Корнилова
Keyword(s):  
Dermal Fibroblast ◽  
Nuclear Antigen ◽  
Positive Staining ◽  
Proliferating Cells ◽  
Age Related ◽  
Age Dependent ◽  
Human Dermis ◽  
Group A ◽  
Arylhydrocarbon Receptor

Целью настоящей работы стало исследование содержания ядерного транслокатора арил-гидрокарбонового рецептора ( ARNT ) в фибробластах дермы человека от 20 нед беременности до 85 лет, а также определение значения ARNT для возрастных изменений численности фибробластов в дерме человека. ARNT , ядерный антиген пролиферирующих клеток ( PCNA ) выявляли в срезах кожи непрямым иммуногистохимическим методом. Результаты показали, что доля фибробластов дермы с положительной окраской на ARNT постепенно снижается от 20 нед беременности до 40 лет. С 41 года происходит резкое увеличение доли фибробластов дермы с положительной окраской на ARNT, достигая максимума в возрастной группе 61-85 лет. Общее количество и доля PCNA -положительных фибробластов в дерме уменьшается с возрастом, наиболее значимо - с антенатального периода до 40 лет. Возрастные изменения содержания ARNT в фибробластах дермы статистически не связаны с возрастным уменьшением численности общего количества и процента PCNA -положительных фибробластов в дерме. The aim of this work was to examine the content of arylhydrocarbon receptor nuclear translocator ( ARNT ) in fibroblasts of human dermis from 20 weeks of pregnancy until 85 years old, and defining of a role of ARNT in age-dependent changes in the number of fibroblasts in the dermis. ARNT , proliferating cells nuclear antigen ( PCNA ) were detected with indirect immunohistochemical technique. Results showed that a portion of fibroblasts with positive staining for ARNT in the dermis is decreased from 20 weeks of pregnancy to 40 years old. Percent of ARNT positive fibroblasts in dermis is increased sufficiently since 41 year old until 60-85 years old group. A total number and percent of PCNA positive fibroblasts in dermis decreased with progression of age. Most sufficient age-dependent reduction in a total and PCNA positive number of dermal fibroblast was observed from antenatal until 40 years of life. Age-related changes in the content of ARNT in fibroblasts is not associated with an age-related decrease in total number and percent of PCNA positive fibroblasts the dermis.

ARYL HYDROCARBON RECEPTOR INTERACTING PROTEIN () IN HUMAN DERMIS DURING AGING

2020 ◽  
pp. 444-449
Author(s):  
А. Г. Гунин ◽  
Н. Н. Голубцова
Keyword(s):  
Aryl Hydrocarbon Receptor ◽  
Dermal Fibroblast ◽  
Nuclear Antigen ◽  
Positive Staining ◽  
Proliferating Cells ◽  
Interacting Protein ◽  
Aryl Hydrocarbon ◽  
Age Related ◽  
Age Dependent ◽  
Human Dermis

Целью настоящей работы стало исследование содержания белка, ассоциированного с арилгидрокарбоновым рецептором, - aryl hydrocarbon receptor interacting protein ( AIP ), в фибробластах дермы человека от 20 нед беременности до 85 лет, а также определение значения AIP для возрастных изменений численности фибробластов в дерме человека. AIP и ядерный антиген пролиферирующих клеток ( PCNA ) выявляли в срезах кожи непрямым иммуногистохимическим методом. Результаты показали, что в коже человека от 20 нед беременности до 85 лет доля фибробластов дермы с положительной окраской на AIP планомерно возрастает. Общее количество и доля PCNA -положительных фибробластов в дерме уменьшались с возрастом. Наиболее значительное возрастное уменьшение общего числа и PCNA -положительных фибробластов в дерме наблюдали с антенатального периода до 40 лет. Корреляционный анализ показал, что возрастное уменьшение общего числа фибробластов и уменьшение их пролиферативной активности достоверно связаны с увеличением процента фибробластов с положительной окраской на AIP . Результаты позволяют предположить, что AIP участвует в возрастном уменьшении численности и пролиферации фибробластов в дерме человека. The aim of this work was to examine the content of aryl hydrocarbon receptor interacting protein ( AIP ) in fibroblasts of human dermis from 20 weeks of pregnancy until 85 years old, and defining of a role of AIP in age-dependent changes in the number of fibroblasts in the dermis. AIP , proliferating cells nuclear antigen ( PCNA ) were detected with indirect immunohistochemical technique. Results showed that a portion of fibroblasts with positive staining for AIP in the dermis is gradually increased from 20 weeks of pregnancy until 85 years old. A total number and percent of PCNA positive fibroblasts in dermis decreased with progression of age. Most sufficient age-dependent reduction in a total and PCNA positive number of dermal fibroblast was observed from antenatal until 40 years of life. Correlation analysis showed that both age-dependent decrease in the number of fibroblasts and retardation of their proliferation are significantly associated with age-related increase in the number of AIP positive fibroblasts in dermis. Results allow to suggest that AIP is involved in age-dependent decrease in the number and proliferation of fibroblasts in human dermis.

CHANGES IN THE NUMBER OF p23-POSITIVE FIBROBLASTS IN HUMAN DERMIS WITH AGING

2021 ◽  
pp. 694-700
Author(s):  
А.Г. Гунин ◽  
Н.Н. Голубцова
Keyword(s):  
Old Age ◽  
Nuclear Antigen ◽  
Positive Staining ◽  
Proliferating Cells ◽  
Immunohistochemical Technique ◽  
Age Related ◽  
Age Dependent ◽  
Human Dermis ◽  
Related Increase

Целью работы стало исследование содержания белка p23 в фибробластах дермы человека от 20 нед беременности до 85 лет, а также определение участия p23 для возрастных изменений численности и пролиферации фибробластов в дерме человека. p23, ядерный антиген пролиферирующих клеток (PCNA), маркер фибробластов виментин выявляли в срезах кожи непрямым иммуногистохимическим методом. В дерме человека от 20 нед беременности до 85 лет наблюдали планомерное увеличение доли фибробластов, имеющих положительную окраску на p23. Возрастное изменение численности p23позитивных фибробластов в дерме достоверно коррелирует с возрастным уменьшением общей численности и доли PCNA-позитивных фибробластов в дерме человека, что свидетельствует об участии p23 в возрастных изменениях общей численности и доли пролиферирующих фибробластов в дерме человека. The aim of this work was to examine the content of p23 in fibroblasts of human dermis from 20 weeks of pregnancy until 85 years old, and defining of a role of p23 in age-dependent changes in the number and proliferation of fibroblasts in the dermis. p23, proliferating cells nuclear antigen (PCNA), fibroblasts marker vimentin were detected with indirect immunohistochemical technique. Results showed that portion of fibroblasts with positive staining for p23 in the dermis is gradually increased from 20 weeks of pregnancy until 85 years old. Age-related increase in a portion of fibroblasts with positive staining for p23 is significantly correlated with an age-related decrease in total number and percent of PCNA positive fibroblasts in human dermis. Age-related increase in the content of p23 in fibroblasts is involved in an age-dependent decrease in their total number and proliferation in the dermis.

HEAT SHOCK PROTEIN 90 (90) IN AGE-DEPENDENT CHANGES IN THE NUMBER OF FIBROBLASTS IN HUMAN SKIN

2020 ◽  
pp. 40-45
Author(s):  
А. Г. Гунин ◽  
Н. Н. Голубцова ◽  
Н. К. Корнилова
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 90 ◽  
Nuclear Antigen ◽  
Positive Staining ◽  
Proliferating Cells ◽  
Age Related ◽  
Age Dependent ◽  
Human Dermis ◽  
Hsp 90

Целью работы стало исследование содержания белка теплового шока 90 ( HSP 90) в фибробластах дермы человека от эмбрионального развития и до глубокой старости (от 20 нед беременности до 85 лет), а также определение значения HSP 90 для возрастных изменений численности фибробластов в дерме человека. HSP 90, ядерный антиген пролиферирующих клеток ( PCNA ) выявляли в срезах кожи непрямым иммуногистохимическим методом. Результаты показали, что в коже человека от 20 нед беременности до 20 лет доля фибробластов дермы с положительной окраской на HSP 90 остается постоянной. С 21 года до 60 лет наблюдают планомерное уменьшение доли фибробластов дермы, имеющих положительную окраску на HSP 90. У людей 61-85 лет происходит резкое увеличение доли фибробластов дермы с положительной окраской на HSP 90. Возрастные изменения содержания HSP 90 положительных фибробластов в дерме статистически не связаны с возрастным уменьшением общего количества и доли PCNA -положительных фибробластов в дерме. The aim of this work was to examine the content of heat shock protein 90 ( HSP 90) in fibroblasts of human dermis from the development until deep aging (from 20 weeks of pregnancy until 85 years old), and defining of a role of HSP 90 in age-dependent changes in the number of fibroblasts in the dermis. HSP 90, proliferating cells nuclear antigen ( PCNA ) were detected with indirect immunohistochemical technique. Results showed that a portion of fibroblasts with positive staining for HSP 90 in the dermis is not changed from 20 weeks of development to 20 years old. Percent of HSP 90 positive fibroblasts in dermis is decreased from 21 to 60 years old. From 61 year, the number of HSP 90 positive fibroblasts in dermis is increased. Age-related changes in the number of HSP 90 positive fibroblasts is not statistically associated with an age-related decrease in a total number and percent of PCNA positive fibroblasts the dermis.

Tissue Ki67 proliferative index expression and pathological changes in hemodialysis arteriovenous fistulae: Preliminary single-center results

2021 ◽  
pp. 112972982110154
Author(s):  
Raffaella Mauro ◽  
Cristina Rocchi ◽  
Francesco Vasuri ◽  
Alessia Pini ◽  
Anna Laura Croci Chiocchini ◽  
...  
Keyword(s):  
Morphological Changes ◽  
Hot Spot ◽  
Wall Thickening ◽  
Proliferating Cells ◽  
Ki 67 ◽  
Group A ◽  
The Mean ◽  
Set Up ◽  
Group B

Background: Arteriovenous fistula (AVF) for hemodialysis integrates outward remodeling with vessel wall thickening in response to drastic hemodynamic changes. Aim of this study is to determine the role of Ki67, a well-established proliferative marker, related to AVF, and its relationship with time-dependent histological morphologic changes. Materials and methods: All patients were enrolled in 1 year and stratified in two groups: (A) pre-dialysis patients submitted to first AVF and (B) patients submitted to revision of AVF. Morphological changes: neo-angiogenesis (NAG), myointimal thickening (MIT), inflammatory infiltrate (IT), and aneurysmatic fistula degeneration (AD). The time of AVF creation was recorded. A biopsy of native vein in Group A and of arterialized vein in Group B was submitted to histological and immunohistochemical (IHC) analysis. IHC for Ki67 was automatically performed in all specimens. Ki67 immunoreactivity was assessed as the mean number of positive cells on several high-power fields, counted in the hot spots. Results: A total of 138 patients were enrolled, 69 (50.0%) Group A and 69 (50.0%) Group B. No NAG or MIT were found in Group A. Seven (10.1%) Group A veins showed a mild MIT. Analyzing the Group B, a moderate-to-severe MIT was present in 35 (50.7%), IT in 19 (27.5%), NAG in 37 (53.6%); AD was present in 10 (14.5%). All AVF of Group B with the exception of one (1.4%) showed a positivity for Ki67, with a mean of 12.31 ± 13.79 positive cells/hot spot (range 0–65). Ki67-immunoreactive cells had a subendothelial localization in 23 (33.3%) cases, a myointimal localization in SMC in 35 (50.7%) cases. The number of positive cells was significantly correlated with subendothelial localization of Ki67 ( p = 0.001) and with NA ( p = 0.001). Conclusions: Native veins do not contain cycling cells. In contrast, vascular cell proliferation starts immediately after AVF creation and persists independently of the time the fistula is set up. The amount of proliferating cells is significantly associated with MIT and subendothelial localization of Ki67-immunoreactive cells, thus suggesting a role of Ki-67 index in predicting AVF failure.

scholarly journals Age - related behaviour on individually caged rabbits

2017 ◽  
Vol 62 (1) ◽  
pp. 21 ◽  
Author(s):  
A. KATSAROU (Α. ΚΑΤΣΑΡΟΥ) ◽  
A. TSIRONI (Α. ΤΣΙΡΩΝΗ) ◽  
M. SERAFETINIDOU (Μ. ΣΕΡΑΦΕΤΙΝΙΔΟΥ) ◽  
C. VOYAZAKI (Χ. ΒΟΓΙΑΤΖΑΚΗ) ◽  
V. BAUMANS ◽  
...  
Keyword(s):  
Age Differences ◽  
Environmental Enrichment ◽  
Housing Conditions ◽  
Enrichment Programs ◽  
Video Recordings ◽  
Age Related ◽  
Age Dependent ◽  
Enrichment Program ◽  
Group A

Housing conditions and environmental enrichment of individually caged laboratory rabbits is of great importance for the welfare of the animals and the quality of the experimental results. In order to improve the design of existing environmental enrichment programs for laboratory rabbits, considerable knowledge of the behavioural needs of this species is necessary. Taking this into consideration, the aim of this study was to monitor and analyze the behaviour of juvenile and young adult rabbits in order to establish whether there are any age-dependent differences in grooming, rearing, sniffing, eating, drinking and gnawing. 12 NZW rabbits were divided into two groups: group A consisted of six 6-month-old rabbits (young adults) and group Β consisted of six 2-month-old rabbits (juvenile). All animals were already housed for more than twenty days under the same conditions in the animal facility. Both groups of rabbits were video-recorded between 06:00h - 18:00h for four consecutive days. The frequency of each behaviour was determined and compared in the two groups of rabbits from the video recordings. The frequencies of grooming, eating and gnawing in the young rabbits were significantly greater than those in the older rabbits (p<0.05). No statistical differences were found between the two groups for rearing, sniffing and drinking. From these results, we concluded that even small age differences should be taken into account when designing an environmental enrichment program for individually caged rabbits.

Age-dependent alterations in Ca2+ homeostasis: role of TRPV5 and TRPV6

2006 ◽  
Vol 291 (6) ◽  
pp. F1177-F1183 ◽  
Author(s):  
Monique van Abel ◽  
Sylvie Huybers ◽  
Joost G. J. Hoenderop ◽  
Annemiete W. C. M. van der Kemp ◽  
Johannes P. T. M. van Leeuwen ◽  
...  
Keyword(s):  
Vitamin D ◽  
Mrna Levels ◽  
Serum Osteocalcin ◽  
Intestinal Epithelial ◽  
Receptor Mrna ◽  
Age Related ◽  
Age Dependent ◽  
Elder People ◽  
Age Related Changes

Aging is associated with alterations in Ca2+ homeostasis, which predisposes elder people to hyperparathyroidism and osteoporosis. Intestinal Ca2+ absorption decreases with aging and, in particular, active transport of Ca2+ by the duodenum. In addition, there are age-related changes in renal Ca2+ handling. To examine age-related changes in expression of the renal and intestinal epithelial Ca2+ channels, control (TRPV5+/+) and TRPV5 knockout (TRPV5−/−) mice aged 10, 30, and 52 wk were studied. Aging of TRPV5+/+ mice resulted in a tendency toward increased renal Ca2+ excretion and significantly decreased intestinal Ca2+ absorption, which was accompanied by reduced expression of TRPV5 and TRPV6, respectively, despite increased serum 1,25(OH)2D3 levels. Similarly, in TRPV5−/− mice the existing renal Ca2+ loss was more pronounced in elder animals, whereas the compensatory intestinal Ca2+ absorption and TRPV6 expression declined with aging. In both mice strains, aging resulted in a resistance to 1,25(OH)2D3 and diminished renal vitamin D receptor mRNA levels, whereas serum Ca2+ levels remained constant. Furthermore, 52-wk-old TRPV5−/− mice showed severe hyperparathyroidism, whereas PTH levels in elder TRPV5+/+ mice remained normal. In 52-wk-old TRPV5−/− mice, serum osteocalcin levels were increased in accordance with the elevated PTH levels, suggesting an increased bone turnover in these mice. In conclusion, downregulation of TRPV5 and TRPV6 is likely involved in the impaired Ca2+ (re)absorption during aging. Moreover, TRPV5−/− mice likely develop age-related hyperparathyroidism and osteoporotic characteristics before TRPV5+/+ mice, demonstrating the importance of the epithelial Ca2+ channels in Ca2+ homeostasis.

scholarly journals Impaired peroxisomal import in Drosophila hepatocyte-like cells induces cardiac dysfunction through the pro-inflammatory cytokine Upd3

2019 ◽  
Author(s):  
Kerui Huang ◽  
Ting Miao ◽  
Kai Chang ◽  
Ping Kang ◽  
Qiuhan Jiang ◽  
...  
Keyword(s):  
Cardiac Dysfunction ◽  
Inflammatory Cytokine ◽  
Cytokine Signaling ◽  
Over Expression ◽  
Autonomous Regulation ◽  
Age Related ◽  
Evolutionarily Conserved ◽  
Age Dependent ◽  
Import Receptor

AbstractAge is a major risk factor for cardiovascular diseases. Currently, the non-autonomous regulation of age-related cardiac dysfunction is poorly understood. In the present study, we discover that age-dependent induction of cytokine unpaired 3 (Upd3) in Drosophila oenocytes (hepatocyte-like cells), due to a dampened peroxisomal import function, is the primary non-autonomous mechanism for elevated arrhythmicity in old hearts. We show that Upd3 is significantly up-regulated (52-fold) in aged oenocytes. Oenocyte-specific knockdown of Upd3 is sufficient to block aging-induced cardiac arrhythmia. We further show that the age-dependent induction of Upd3 is triggered by impaired peroxisomal import and elevated JNK signaling in aged oenocytes. Intriguingly, oenocyte-specific over-expression of Pex5, the key peroxisomal import receptor, restores peroxisomal import, blocks age-related Upd3 induction, and alleviates aging- and paraquat-induced cardiac arrhythmicity. Thus, our studies identify an important role of the evolutionarily conserved pro-inflammatory cytokine signaling and hepatocyte-specific peroxisomal import in mediating non-autonomous regulation of cardiac aging.

scholarly journals Immunosenescence in Choroidal Neovascularization (CNV)—Transcriptional Profiling of Naïve and CNV-Associated Retinal Myeloid Cells during Aging

2021 ◽  
Vol 22 (24) ◽  
pp. 13318
Author(s):  
Anja Schlecht ◽  
Adrian Thien ◽  
Julian Wolf ◽  
Gabriele Prinz ◽  
Hansjürgen Agostini ◽  
...  
Keyword(s):  
Choroidal Neovascularization ◽  
Transcriptional Profiling ◽  
Myeloid Cells ◽  
Age Related Macular Degeneration ◽  
Physiological Aging ◽  
Future Studies ◽  
Signature Genes ◽  
Age Related ◽  
Age Dependent

Immunosenescence is considered a possible factor in the development of age-related macular degeneration and choroidal neovascularization (CNV). However, age-related changes of myeloid cells (MCs), such as microglia and macrophages, in the healthy retina or during CNV formation are ill-defined. In this study, Cx3cr1-positive MCs were isolated by fluorescence-activated cell sorting from six-week (young) and two-year-old (old) Cx3cr1GFP/+ mice, both during physiological aging and laser-induced CNV development. High-throughput RNA-sequencing was performed to define the age-dependent transcriptional differences in MCs during physiological aging and CNV development, complemented by immunohistochemical characterization and the quantification of MCs, as well as CNV size measurements. These analyses revealed that myeloid cells change their transcriptional profile during both aging and CNV development. In the steady state, senescent MCs demonstrated an upregulation of factors contributing to cell proliferation and chemotaxis, such as Cxcl13 and Cxcl14, as well as the downregulation of microglial signature genes. During CNV formation, aged myeloid cells revealed a significant upregulation of angiogenic factors such as Arg1 and Lrg1 concomitant with significantly enlarged CNV and an increased accumulation of MCs in aged mice in comparison to young mice. Future studies need to clarify whether this observation is an epiphenomenon or a causal relationship to determine the role of immunosenescence in CNV formation.

Role of microRNA-24 Transfection Mediated by Silica Nanoparticles in Apoptosis of Cardiomyocyte

2021 ◽  
Vol 13 (3) ◽  
pp. 455-462
Author(s):  
Zonghu Wu
Keyword(s):  
Particle Size ◽  
Silica Nanoparticles ◽  
Transfection Efficiency ◽  
Cell Uptake ◽  
Control Group ◽  
Tunel Staining ◽  
Positive Staining ◽  
Group A ◽  
Group B

To explore the role of micro RNA-24 (miR-24) mediated by silica nanoparticles (SiNPs) modified by polyethyleneimine (PEI) on apoptosis of cardiomyocyte induced by H2O2, in this study, PEI was selected to modify the surface of SiNPs to obtain F-SiNPs, which were combined with miR-24 through electrostatic adsorption to form F-SiNPs-miR-24. Through characterization, the particle size, charge amount, and microstructure were studied. Then, the CCK-8 method was adopted to detect the toxicity of F-SiNPs on cardiomyocytes, and the cell uptake experiment was performed to analyze the transfection efficiency of F-SiNPs-miR-24 gene. There were 4 groups in vitro transfection experiment: the control group (A), the H2O2 group (B), the miR-24 inhibitor group (C), and miR-24 analog group (D). After that, RT-PCR was adopted to detect the expression of miR-24, Western blotting was used to detect the expression of target protein Bim, and the apoptosis of cardiomyocytes was analyzed by TUNEL staining. The results showed that the particle size of SiNPs was mainly distributed in the range of 40-60 nm, the mass ratio of SiNPs:DSP-PEI was 1:1, and the potential was 20.15 ±2.04 (mv) to construct functionalized nanoparticles. Cell uptake experiments showed that the gene transfection efficiency reached over 85%. In contrast with group A, the expression of miR-24 in B and C was obviously reduced, and that in D was obviously increased (P < 0.05). In contrast with group B, the expression of miR-24 in C was obviously reduced, and that in D was obviously increased (P < 0.05). The expression level of protein Bim in groups B and C was obviously higher than A (P < 0.05). In contrast with group A, the TUNEL-positive staining cells in C increased obviously and were bright green, while those in B were obviously reduced. In conclusion, the F-SiNPs formed after energetic modification have good biocompatibility and strong transfection ability, and their miR-24 transfection can inhibit H2O2-induced cardiomyocyte apoptosis and increase the survival rate of cardiomyocytes.

Immunohistochemical Expression of Alpha (Α) A Crystallin in Senile Degenerative and Non-Cataract Lenses

2021 ◽  
Vol 15 (10) ◽  
pp. 2643-2646
Author(s):  
Shabnam Khan ◽  
Bushra Wasim Khan ◽  
Madeeha Sadiq ◽  
Fawad Rizvi ◽  
Faraz Ahmed Baig ◽  
...  
Keyword(s):  
Cross Sectional Study ◽  
Senile Cataract ◽  
Cross Sectional ◽  
Lens Fibers ◽  
Positive Expression ◽  
Age Related ◽  
Keywords Immunohistochemistry ◽  
Group A ◽  
Group B

Aim: Comparative immunohistochemical study of expression of α A Crystallin in non-cataract lenses and age-related cataract lenses in humans. Methodology: This was an observational cross sectional study. There are two groups in this study. Group A comprised of 121 senile degenerative cataract lenses from diagnosed patients. Group B included of 10 non-cataract lenses from patients who underwent surgeries for enucleation due to trauma and retinoblastoma. Lenses were fixed in 10% Buffered Neutral Formalin and processed to make paraffin blocks. Immunohistochemistry (IHC) staining was performed on sections using primary antibody for α A crystallin. Data was analyzed through SPSS software version 24. Results: Immunohistochemical staining of group A showed 80.2% Strong Positive expression while 19.8% showed Intermediate Positive expression of α A Crystallin. 100% Strong Positive expression of α A Crystallin was seen in group B. Comparison of expression of α A Crystallin in two groups showed significant decrease (p<0.001) in expression. Conclusion: Decreased expression of α A Crystallin in IHC stained senile cataract lens indicates the role of structural alterations of lens fibers in pathogenesis of senile cataract. If mechanism involved in causing these alterations can be identified and targeted so that progression of senile cataract may be delayed. Keywords: Immunohistochemistry, α A crystallin expression, senile cataract, Human eye Lens, Lens Fiber.

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