ANTIBAKTERI SENYAWA FENOLIK DARI ALANG -ALANG (IMPERATA CYLINDRICA)

Phytochemical screening test proved that the extract of Reeds (Imperata cylindrica) contained phenolic compounds tested using 5% FeCl3 reagent. Antibacterial test using agar diffusion method against Reeds extract in DMSO solvent. The phenolic compounds obtained from the saputangan leaves Reeds extract were 36.96 grams after undergoing maceration. Reeds extract of phenolic compounds with concentrations (200; 100; 50 and 25 ppm) had strength antibacterial where the average inhibition zone of Staphylococcus aureus bacteria was 10.0 mm and the average inhibition zone was at Escherichia coli bacteria measuring 10.3 mm.

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UJI AKTIVITAS ANTIBAKTERI DAUN JERUK KALAMANSI (Citrofortunella microcarpa) TERHADAP BAKTERI Staphylococcus aureus DAN Escherichia coli

Alotrop ◽

10.33369/atp.v3i2.10122 ◽

2019 ◽

Vol 3 (2) ◽

Author(s):

Deza Oktasila ◽

Nurhamidah Nurhamidah ◽

Dewi Handayani

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Essential Oil ◽

Ethanol Extract ◽

Inhibition Zone ◽

Raw Material ◽

Diffusion Method ◽

Citrus Leaves ◽

Escherichia Coli Bacteria

This study aims to examine the antibacterial activity of ethanol extract and essential oil of Kalamansi citrus leaves (Citrofortunella microcarpa) against Staphylococcus aureus and Escherichia coli bacteria. The raw material of Kalamansi citrus leaves is obtained from the Village of Pondok Kubang, Bengkulu Tengah (3.70060S, 102.35780E). Ethanol extract from Kalamansi citrus leaves was obtained by maceration using ethanol 96%, then ethanol extract was made dilution concentration 40 ; 20; , 10; and 5%. The essential oil of Kalamansi citrus leaves is obtained by water-vapor distillation, then made variations of concentration 20;, 15;, 10; and 5%. The method used to test the antibacterial activity is the paper disc diffusion method , the antibacterial activity is shown by the diameter of the inhibiting zone formed. The data of antibacterial test result were analyzed by using One Way Anova test which showed the effect of treatment on the growth of test bacteria seen from the value (P <0,01) and continued by Duncan test to know the effect of the treatment. The results showed that ethanol extract of Kalamansi citrus leaves had antibacterial activity against S. aureus and E. coli bacteria with moderate inhibitory diameter is 7.20 and 5.73 mm at concentration 40%, while antibacterial activity on essential oil of Kalamansi citrus leaves is categorized as strong with inhibition zone diameter is 14.83 and 13.00 mm at concentration 20%.

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ANTIBAKTERI SENYAWA FENOLIK DARI DAUN SAPUTANGAN (Maniltoa Grandiflora (A. Gray) Scheff)

Jurnal Penelitian Farmasi & Herbal ◽

10.36656/jpfh.v1i2.64 ◽

2019 ◽

Vol 1 (2) ◽

pp. 17-21

Author(s):

JHON PATAR SINURAT ◽

SAADAH SIREGAR

Keyword(s):

Phenolic Compound ◽

Inhibition Zone ◽

Diffusion Method ◽

Phytochemical Screening ◽

Inhibitory Zone ◽

E Coli ◽

Medium Level ◽

Agar Diffusion Method ◽

Escherichia Coli Bacteria ◽

Positive Results

The Phytochemical screening test to identify secondary metabolites of Saputangan leaves extract (Maniltoa grandiflora (A.Gray) Scheff) showed positive results on phenolic and terpenoid compounds. The reagent used for phenolic screening was 5% FeCl3 and reagent for screening terpenoid was 1% CeSO4 in 10% H2SO4. Antibacterial test was carried out by agar diffusion method on extracts of DMSO Saputangan leaves. Phenolic compound obtained from extract of Saputangan leaves were 36.96 gram where the result was obtained after maceration, partitioning and evaporation processes. Antibacterial of phenolic compound was observed based on inhibitory zone diameters of phenolic compounds formed using paper discs with a diameter of 6 mm. The diameters of the inhibition zone against Escherichia coli bacteria are 8.1; 8.5 and 9.7 mm larger than the diameter of the inhibition zone in Staphylococcus aureus bacteria at 7.3; 7.8 and 8.5 mm. Then it can be stated that DMSO extracts of phenolic compound with concentrations (0.25; 0.50 and 1.00 mg / ml) have antibacterial strength at the medium level where the average inhibition zone of S. aureus bacteria is 7.87 mm and the average inhibition zone in E. coli bacteria is 8.76 mm.

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UJI AKTIVITAS ANTIMIKROBA EKSTRAK DAN FRAKSI SPONS Phyllospongia lamellosa DARI PERAIRAN TUMBAK, MINAHASA TENGGARA TERHADAP PERTUMBUHAN MIKROBA Escherichia coli, Staphylococcus aureus DAN Candida albicans

PHARMACON ◽

10.35799/pha.8.2019.29358 ◽

2019 ◽

Vol 8 (4) ◽

pp. 815

Author(s):

Kartini Ratu ◽

Herny E. I. Simbala ◽

Henki Rotinsulu

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Candida Albicans ◽

Inhibition Zone ◽

Diffusion Method ◽

Average Value ◽

Activity Test ◽

Paper Disk ◽

Agar Diffusion Method

ABSTRACT Sponges are a component of coral reef biota. These sea animals are known to contain compounds that have the potential to be developed in the field of medicine, including as an antimicrobial. This study aims to determine the antimicrobial activity of Phyllospongia lamellosa sponge against the growth of Staphylococcus aureus, Escherichia coli and Candida albicans collected in the waters of Tumbak, Posumaen District, Southeast Minahasa. The antimicrobial activity test was carried out by agar diffusion method. The results showed that the extract and fraction of Phyllospongia lamellose had antimicrobial activity seen in the inhibition zone formed around the paper disk against the test microbes. Ethanol extrack and fraction from Phyllospongia lamellosa sponge showed the greatest antimicrobial activity against Candida albicans with an average value of 13,33 mm was categorized as strong , than in Staphylococcus aureus with an average value of 13 mm is categorized as strong and on Escherichia coli 11 mm categorized as strong. Keywords :Phyllospongia lamellosa, antimicrobial activity, Escherichia coli, Staphylococcus aureus, Candida albicans ABSTRAK Spons merupakan salah satu komponen biota penyusun terumbu karang. Hewan laut ini diketahui mengandung senyawa- senyawa yang berpotensi untuk dikembangkan dalam bidang pengobatan, diantaranya sebagai antimikroba. Penelitian ini bertujuan untuk mengetahui aktivitas antimikroba spons Phyllospongia Lamellosa terhadap pertumbuhan Staphylococcus aureus, Escherichia coli dan Candida albicans yang diambil pada perairan Tumbak Kecamatan Posumaen, Minahasa Tenggara. Uji aktifitas antimikroba dilakukan dengan metode difusi agar. Hasil penelitian menunjukkan bahwa ekstrak dan fraksi spons Phyllospongia lamellose memiliki aktifitas antimikroba dilihat zona hambat yang terbentuk disekitar cakram kertas terhadap mikroba uji. Ekstrak etanol dan fraksi dari Spons Phyllospongia lamellosa menunjukkan aktivitas antimikroba paling kuat terhadap candida albicans dengan nilai rata-rata 13,33 mm dikategorikan kuat, kemudian pada Staphylococcus aureus dengan nilai rata-rata 13 mm dikategorikan kuat, dan pada Escherichia coli 11 mm dikategorikan kuat. Kata Kunci : Phyllospongia lamellosa, aktivitas antimikroba, Escherichia coli, Staphylococcus aureus, Candida albicans

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Fitokimia Dan Aktivitas Antibakteri Dari Daun KAF(Chisocheton sp. (C. DC) HARMS)

Jurnal MIPA ◽

10.35799/jm.8.2.2019.23514 ◽

2019 ◽

Vol 8 (2) ◽

pp. 42

Author(s):

Florentin Natalia Melsadalam ◽

Dewa Gede Katja ◽

Meiske S. Sangi

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Secondary Metabolites ◽

Ethyl Acetate ◽

Leaf Extract ◽

Inhibition Zone ◽

Diffusion Method ◽

Inhibitory Zone ◽

Escherichia Coli Bacteria

Telah dilakukan penelitian yang bertujuan untuk mengidentifikasi senyawa metabolit sekunder dari ekstrak daun kaf menggunakan tiga jenis pelarut yakni n-heksan, etil asetat, dan metanol. Ekstrak yang diperoleh diuji aktivitas antibakterinya dengan metode difusi yang menggunakan bakteri Staphylococcusaureus dan Escherichia coli. Dari hasil pengujian fitokimia, ketiga ekstrak tersebut menunjukan bahwa sampel mengandung senyawa; alkaloid, saponin, steroid, flavonoid dan tanin. Pengujian aktivitas antibakteri dengan metode difusi menunjukan hasil yang paling tinggi aktivitas antibakteri adalah estrak etil asetat dengan zona hambat 35,75 mm terhadap bakteri Staphylococcus aureus, sedangkan pada ekstrak metanol memiliki zona hambat 23,75 mm terhadap bakteri Escherichia coliResearch has been carried out aimed at identifying secondary metabolites of kaf leaf extract using three types of solvents, namely n-hexane, ethyl acetate, and methanol.The obtained extracts were tested for antibacterial activity by diffusion method using Staphylococcus aureus and Escherichia coli bacteria. From the results of phytochemical testing, the three extracts showed that the sample contained compounds; alkaloids, saponins, steroids, flavonoids and tannins. Antibacterial activity testing with diffusion method showed the highest antibacterial activity was estracethyl ethyl acetate with 35.75 mm inhibition zone against Staphylococcus aureus bacteria, whereas methanol had 23.75 mm inhibitory zone against Escherichia coli bacteria

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Antibacterial substances of sponges, Agelas tubulata and Phyllospongia sp., from Manado Bay, against the growth of several bacterial strains

AQUATIC SCIENCE & MANAGEMENT ◽

10.35800/jasm.5.1.2017.24253 ◽

2019 ◽

Vol 5 (1) ◽

pp. 23

Author(s):

Nani I.J Undap ◽

Deiske A Sumilat ◽

Robert Bara

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Proteus Mirabilis ◽

Inhibition Zone ◽

Diffusion Method ◽

Active Fraction ◽

Bacterial Strains ◽

Agar Diffusion Method ◽

Bahasa Indonesia

Title (Bahasa Indonesia): Senyawa antibakteri spons, Agelas tubulata dan Phyllospongia sp., dari Perairan Teluk Manado, terhadap pertumbuhan beberapa strain bakteri Agelas tubulataand Phyllospongiasp. are marine spongethat contain active compoundsthat have potential to beantibacterial, anticancer and antifungal which have not been used. This study was aimed to examine the sponge extracts which inhibitbacterial growth, i.e. Pseudomonas aeruginosa, Proteus mirabilis, Staphylococcus aureus, Escherichia coli, S. saprophyticus andtoanalyze the active fraction of the antibacterial component using bio-autographic method. Agelas tubulataand Phyllospongiasp. were collected from Manado Bayand method used in the study was Agar Diffusion Method. Results showed that A. tubulataand Phyllospongiasp. extract had antibacterial activity against all 5 bacteria tested. A. tubulataextract had inhibition zone withbroader spectrum than that of Phyllospongiasp. extract, while Phyllospongiasp. has specifity forP. mirabilis.Agelas tubulata danPhyllospongia sp. merupakan spesies spons laut yang mengandung senyawa aktif yang berpotensi antibakteri, antikanker dan antijamur yang belum banyak dimanfaatkan. Penelitian ini bertujuan untuk menguji ekstrak spons laut yang menghambat pertumbuhan 5 bakteri yaitu Pseudomonas aeruginosa, Proteus mirabilis, Staphylococcus aureus, Eschericia colidan Staphylococcus saprophyticus dan menganalisis fraksi aktif antibakteri melalui teknik bioautografi. Spesies spons laut A. tubulata dan Phyllospongiasp. diambil dari Perairan Pantai Malalayang Manado. Metode yang digunakan yaitu metode difusi agar. Hasil dari penelitian ini, yaitu ekstrak A. tubulatadan Phyllospongiasp. mempunyai aktivitas terhadap 5 bakteri uji. EkstrakA. tubulatamemiliki daya hambat dengan spektrum yang lebih luas dibandingkan ekstrak Phyllospongiasp.sedangkanekstrak Phyllospongia sp.memiliki spesifitas terhadap bakteri P. mirabilis.

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AKTIVITAS ANTIMIKROBA EKSTRAK DAN FRAKSI ALGA (Halimeda opuntia) TERHADAP Escherichia coli, Staphylococcus aureus dan Candida albicans

PHARMACON ◽

10.35799/pha.8.2019.29252 ◽

2019 ◽

Vol 8 (1) ◽

pp. 178

Author(s):

Shinta Jolanda ◽

Defny S Wewengkang ◽

Imam Jayanto

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Candida Albicans ◽

Green Algae ◽

Bioactive Compounds ◽

Ethanol Extract ◽

Diffusion Method ◽

Agar Diffusion Method ◽

Escherichia Coli Bacteria

ABSTRACT Algae Halimeda opuntia is one of the green algae type that contains bioactive compounds which can be used for the treatment of various diseases. This study aimed to determine the antimicrobial activity of extracts and fractions of algae Halimeda opuntia obtained from the Lembeh Bitung Strait against Escherichia coli, Staphylococcus aureus and Candida albicans. The samples were extracted by maceration using ethanol solvent and fractionation using methanol, chloroform, and n-hexane solvents. Antimicrobial activity was carried out by the agar diffusion method of paper discs. The results showed that ethanol extract was able to inhibit the growth of Staphylococcus aureus and Escherichia coli bacteria in the medium inhibitory category. While the methanol and chloroform fractions were only able to inhibit the growth of Staphylococcus aureus bacteria. The extract and all fractions did not show activity inhibiting the growth of Candida albicans fungi. Keywords: Algae, Halimeda opuntia, antimicrobial activity, Escherichia coli, Staphylococcus aureus, Candida albicans ABSTRAKAlga Halimeda opuntia merupakan salah satu jenis alga hijau yang memiliki kandungan senyawa bioaktif yang dapat digunakan untuk pengobatan berbagai penyakit. Penelitian ini bertujuan untuk mengetahui adanya aktivitas antimikroba dari ekstrak dan fraksi alga Halimeda opuntia yang diperoleh dari Selat Lembeh Bitung terhadap Escherichia coli, Staphylococcus aureus dan Candida albicans. Sampel diekstraksi dengan metode maserasi menggunakan pelarut etanol dan fraksinasi menggunakan pelarut metanol, kloroform, dan n-heksan. Aktivitas antimikroba dilakukan dengan metode difusi agar cakram kertas. Hasil penelitian menunjukkan bahwa ekstrak etanol, mampu menghambat pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli dengan kategori daya hambat sedang. Sedangkan fraksi metanol dan fraksi kloroform hanya mampu menghambat pertumbuhan bakteri Staphylococcus aureus. Ekstrak dan semua fraksi tidak menunjukkan aktivitas menghambat pertumbuhan jamur Candida albicans. Kata kunci: Alga, Halimeda opuntia, aktivitas antimikroba, Escherichia coli, Staphylococcus aureus, Candida albicans

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Antibacterial Activity of Combination of Ethanol Extract of Pepermine Leaves (Mentha piperita L.) and Amikacin Against Klebsiella pneumonia, Staphylococcus aureus, and Escherichia coli

Journal of Nutraceuticals and Herbal Medicine ◽

10.23917/jnhm.v4i1.15649 ◽

2021 ◽

Vol 4 (1) ◽

pp. 12-29

Author(s):

Peni Indrayudha

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Screening Test ◽

Ethanolic Extract ◽

Ethanol Extract ◽

Inhibition Zone ◽

Mentha Piperita ◽

Klebsiella Pneumonia ◽

Phytochemical Screening

Peppermint leaves have been known to have antibacterial and antifungal activity. Amikacin is a semisynthetic derivative of kanamycin which is active against both Gram-positive and Gram-negative enteric bacteria. The combination of plant extracts with antibiotics is one way or alternative to overcome bacterial resistance to antibiotics. The purpose of this study was to determine the combined effect of the ethanolic extract of peppermint (Mentha piperita L.) and amikacin against Klebsiella pneumonia, Staphylococcus aureus, and Escherichia coli as well as the compounds contained in the ethanolic extract of peppermint (Mentha piperita L.) leaves. Antibacterial activity was tested using the disk diffusion method (Kirby Bauer) and the phytochemical screening test using the tube test method. The concentration of peppermint leaf ethanol extract for the combination test was 200 mg/mL and 400 mg/mL, for the concentration of amikacin used was 5 mg/mL with three comparisons made, namely 25:75, 50:50, and 75:25. The results showed a synergistic effect with the largest inhibition zone diameter at a ratio of 25:75 at a concentration of 200 mg/mL, which was 36.25±2.5 mm on Klebsiella pneumonia bacteria. While at a concentration of 400 mg/mL the diameter of the largest inhibition zone was 40±1.63 mm in Klebsiella pneumoniae bacteria with a ratio of 75:25. The results of statistical tests using the t-test showed a significance value of 0.000 0.05 so that there was a significant difference in the administration of each concentration to the resulting inhibition zone. The results of the phytochemical screening test contained alkaloids, phenolic compounds, flavonoids, tannins.

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ANTIBAKTERI SENYAWA FENOLIK DARI DAUN SAPUTANGAN (Maniltoa Grandiflora (A. Gray) Scheff)

Jurnal Penelitian Farmasi & Herbal ◽

10.36656/jpfh.v1i2.104 ◽

2019 ◽

Vol 1 (2) ◽

pp. 17-21

Author(s):

Jhon Patar Sinurat ◽

Saadah Siregar

Keyword(s):

Phenolic Compound ◽

Inhibition Zone ◽

Diffusion Method ◽

Phytochemical Screening ◽

Inhibitory Zone ◽

E Coli ◽

Medium Level ◽

Agar Diffusion Method ◽

Escherichia Coli Bacteria ◽

Positive Results

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Antibacterial Activity Test of the C-4-methoxyphenylcalix[4]resorcinarene Compound Modified by Hexadecyltrimethylammonium-Bromide against Staphylococcus aureus and Escherichia coli Bacteria

JKPK (Jurnal Kimia dan Pendidikan Kimia) ◽

10.20961/jkpk.v3i3.22742 ◽

2018 ◽

Vol 3 (3) ◽

pp. 201 ◽

Cited By ~ 1

Author(s):

Suryadi Budi Utomo ◽

Mita Fujiyanti ◽

Warih Puji Lestari ◽

Sri Mulyani

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Inhibition Zone ◽

Positive Control ◽

Negative Control ◽

Diffusion Method ◽

Hexadecyltrimethylammonium Bromide ◽

E Coli ◽

Escherichia Coli Bacteria

This study aims to determine the antibacterial activity of C-4-methoxyphenylcalix-[4]resorcinarene modified by Hexadecyltrimethylammonium-Bromide (Resorcinarene-HDTMA-Br) against Staphylococcus aureus and Escherichia coli bacteria. The study was conducted by testing the inhibition zone diameter with paper disc diffusion method. Variations in the concentration of the sample used were 10%; 15%; 20%; 25%; and 30% b/v. In this study, Dimethyl sulfoxide (DMSO) (sampel solvent) was used as a negative control and Chloramphenicol as a positive control. It was found that Resorsinarene-HDTMA-Br had higher antibacterial activity than the unmodified C-4-methoxyphenylcalix[4]resorcinarene (CMFKR) against the S. aureus bacteria. Both CMFKR and Resorcinarene-HDTMA-Br are not active against E. coli.

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Uji Efek Kombinasi Amoksisilin Dengan Ekstrak Etanol Daun Sirih (Piper Betle L) Terhadap Pertumbuhan Bakteri Escherichia Coli Dan Staphylococcus Aureus

Talenta Conference Series Tropical Medicine (TM) ◽

10.32734/tm.v1i1.67 ◽

2018 ◽

Vol 1 (1) ◽

pp. 313-319

Author(s):

Panal Sitorus

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Water Content ◽

Ethanol Extract ◽

Inhibition Zone ◽

Ash Content ◽

Diffusion Method ◽

Phytochemical Screening ◽

Extract Content ◽

And Staphylococcus Aureus

Konsep baru untuk pengobatan infeksi bakteri resisten adalah dengan kombinasi antibiotika dengan ekstrak herbal. Ekstrak daun sirih memiliki aktivitas anti bakteri terhadap Escherichia coli dan Staphylococcus aureus kerena daun sirih mengandung metabolit sekunder. Kombinasi ekstrak daun sirih dengan amoksisilin, perlu diuji untuk mengetahui efek antibaktreri masing-masing dibandingkan dengan kombinasinya. Mengetahui karakteristik dan skrining fitokimia simplisia dan ekstrak daun sirih serta uji aktivitas anti bakteri kombinasi amoksisilin dengan ekstrak daun sirih terhadap Escherichia coli dan Staphylococcus aureus. Penelitian ini meliputi ekstraksi serbuk simplisia dengan maserasi, Karakterisasi simplisia untuk mengetahui kadar air, kadar sari larut dalam air, kadar sari larut dalam etanol, kadar abu total dan kadar abu tidak larur asam, serta skrining fitokimia simplisia dan ekstrak daun sirih serta uji aktivitas anti bakteri kombinasi amoksisilin dengan ekstrak daun sirih terhadap Escherichia coli dan Staphylococcus aureus dengan metode difusi agar menggunakan cakram kertas. Hasil penelitian menunjukkan bahwa serbuk simplisia daun sirih memiliki kadar air 3,66%; , kadar sari larut dalam air 22,54%, kadar sari larut dalam etanol 14,65%, kadar abu total9,25% dan kadar abu tidak larur asam 1,03%. Komponen kimia pada simplisia dan ekstrak etanol serbuk daun sirih adalah flavonoid, tannin, saponin dan steroid. Ekstrak etanol serbuk daun sirih menghambat pertumbuhan bakteri Escherichia coli dan Staphylococcus aureus masing-masing pada KHM 8 mg/ml dan 9 mg/ml. Amoksisilin menghambat pertumbuhan bakteri Escherichia coli dan Staphylococcus aureus masing-masing pada KHM 5 g/ml dan 10 g/ml dengan diameter zona hambat 6,83mm dan 7,05 mm. Diameter zona hambat terhadap Escherichia coli dan Staphylococcus aureus meningkat ketika bakteri uji dipaparkan dengan kombinasi ekstrak etanol daun sirih dengan amoksisilin. Dari hasil penelitian menunjukkan bahwa kombinasi Amoksisilin dengan Ekstrak etanol daun sirih menunjukkan efek yang sinergis. A new concept to treat resistant bacterial infection is using antibiotic combination with herbal extract. Betel leaves extract has an anti-bacteria activity towards Escherichia coli and Staphylococcus aureus since it contains secondary metabolite. Betel leavesextract combination with amoxicillin is needed to be tested to find evaluatetheiranti-bacteria effects and compare with its combination. By finding the characteristics and phytochemical screening simplicia and the betel leaves extract and also the anti-bacteria activity of amoxicillin combination with betel leaves to Escherichia coli and Staphylococcus aureus. This research covered extraction of simplicia powder using macerationmethod. The simplicia characteristics were conductedto determinethe water content, dissolved extract content in water, dissolved extract content in ethanol, total ash, and the acidic undissolved ash, and also the phytochemical screening of simplicia and the betel leaves extract and also the anti-bacteria activity of amoxicillin combination with betel leaves to Escherichia coli and Staphylococcus aureus using diffusion method using paper disc method. The result indicated that the betel leaves powder simplicia had 3.66% of water content, 22.54% of dissolved content extract in water, 14.65% of dissolved content extract in ethanol, 9.25% of ash content, and 1.03% of acidic undissolved ash content. The chemical components in simpliciaand ethanol extract of betel leaves were flavonoid, tannin, saponin, and steroid. The ethanol extract of betel leaves inhibited the growth of Escherichia coli and Staphylococcus aureus bacteria with MIC of 8 mg/ml and 9 mg/ml. Amoxicillin inhibited the growth of Escherichia coli and Staphylococcus aureus bacteria with MIC of5 g/ml and 10 g/ml with diameter of inhibition zone of 6.83 mm and 7.05 mm, respectively. The diameter of inhibition zone towards Escherichia coli and Staphylococcus aureus increased when the bacteria exposed to the combination of betel leaves ethanol extract and Amoxicillin. The research indicated that Amoxicillin combination with betel leaves ethanol extract showed synergistic effect.

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