scholarly journals Antibiotic Sensitivity of Vibrio cholerae Non-O1/Non-O139 Serogroups Isolated from Aquatic Ecosystems

2020 ◽  
pp. 52-56
Author(s):  
E.A. Bereznyak ◽  
A.V. Trishina ◽  
I.V. Arhangelskaya ◽  
I.R. Simonova ◽  
O.S. Chemisova
Keyword(s):  
Aquatic Ecosystems ◽  
Antibiotic Sensitivity ◽  
Multidrug Resistant ◽  
Wide Spread ◽  
Antimicrobial Drugs ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
Resistant Strains ◽  
Resistance Spectrum ◽  
The City

Introduction: The wide spread of V. cholerae non-O1/non-O139 strains resistant to antibacterial drugs and the variability of antibiotic resistance spectrum are of interest and require regional monitoring studies. Our objective was to accumulate basic information on the state of antibiotic sensitivity/resistance of V. cholerae non-O1/non-O139 strains isolated from water bodies in the city of Rostov-on-Don. Materials and methods: Water samples were taken at stationary sites of open reservoirs from May through September 2016–2018. We established sensitivity/resistance of V. cholerae non-O1/non-O139 strains to antibiotics recommended for emergency prevention and treatment of cholera by their serial dilution in Mueller-Hinton agar. Results: We found that representatives of O16 and O76 serogroups prevailed among 361 isolated V. cholerae non-O1/non-O139 strains during the study period. Monoresistant phenotypes were represented by furazolidone-resistant strains. Conclusions: We established a statistically significant increase in the percentage of strains resistant to nalidixic acid (from 4.0% to 13.3%) and chloramphenicol (from 0.5% to 4.4%) in 2016–2018. Resistance to those two antimicrobial drugs was observed in more than one third of the strains. The most common phenotypes were co-trimoxazole/furazolidone and furazolidone/ampicillin. In 2016, the percentage of such phenotypes was 21.4% and 14.8%, in 2017 – 20.0% and 10.6%, and in 2018 – 20.0% and 15.5%, respectively. When analyzing the microorganisms isolated in 2016–2018 and sensitive to three or more antibiotics, we established significant differences indicating the growth of multidrug-resistant microorganisms belonging to V. cholerae non-O1/non-O139 serogroups.

scholarly journals Strain diversity and antibiotic-sensitivity of staphylococcus spp. Isolates from patients of multiprofile pediatric hospital in St. Petersburg, Russia

2021 ◽  
Vol 12 (4) ◽  
pp. 15-25
Author(s):  
D. P. Gladin ◽  
A. R. Khairullina ◽  
A. M. Korolyuk ◽  
N. S. Kozlova ◽  
O. V. Ananyeva ◽  
...  
Keyword(s):  
Antimicrobial Agents ◽  
Antibiotic Sensitivity ◽  
Specific Weight ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Therapeutic Effects ◽  
Antimicrobial Drugs ◽  
Antibiotic Resistant ◽  
Resistant Strains ◽  
Staphylococcus Spp

Background. Staphylocci are the leading pus-forming Gram-positive bacteria in the childrens hospitals. The prevalence of the antibiotic resistant strains among them limits therapeutic effects of infections in children. Aim. The research is aimed at characterizing the species of staphylococcus, which are isolated from the different clinical specimens of the patients at the clinics of Saint Petersburg State Pediatric Medical University in 2019, and analysis of their susceptibility to antimicrobial agents. Materials and metods. According to the clinical recommendations of 2018, susceptibility to antimicrobial drugs (AMD) was revealed in 860 strains of staphylococci determined by the disc diffusion method, which were identified by the automated analyser Vitek-2 compact. Results. Six species of staphylococci were represented at the hospital departments, among which Staphylococcus epidermidis prevailed in the departments of the neonate pathology department and intensive care units (63.0% and 46.2% respectively), Staphylococcus aureus is commonly found at the departments of surgery and the departments of the therapeutic profiles (61.7% and 46.2% respectively). More than a half of the staphylococci strains (63.0%) were resistant to at least one of the antimicrobial drugs. Vancomycin and line solid showed the highest activity to these staphylococci. High specific weight of multidrug resistant (MDR) bacteria (37.8%) and extensively drug resistant (XDR) strains of the phenotype (33.0%) was revealed. The level of antibiotic resistant strains was the highest in Staphylococcus haemolyticus (98.1%) and S. epidermidis (82.0%), while the specific weight of the resistant ones, MDR and XDR strains was extremely low among S. aureus (16.2%, 1.5% and 0.4 respectively), as well as in methicillin-resistant isolates (0.8%). Conclusions. A great variety of antibiotic resistance was revealed among the staphylococci. The prevalence of these strains in the pediatric hospitals requires constant local monitoring of the antibiotic resistant staphylococci.

scholarly journals The peculiarities of Pseudomonas aeruginosa resistance to antibiotics and prevalence of serogroups

Medicina ◽  
2007 ◽  
Vol 43 (1) ◽  
pp. 36 ◽  
Author(s):  
Greta Gailienė ◽  
Alvydas Pavilonis ◽  
Violeta Kareivienė
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Clinical Laboratory ◽  
Multidrug Resistant ◽  
National Committee ◽  
Clinical Specimens ◽  
Resistance To Antibiotics ◽  
Microdilution Method ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
Resistant Strains

Pseudomonas aeruginosa is one of the most common nonfermenting aerobic gramnegative microorganisms identified in clinical specimens of hospitalized patients. The emergence of multidrug-resistant (MDR) Pseudomonas aeruginosa strains is a growing concern in hospitalacquired infections. Typing of strains is important for identifying the sources of infection as well as prevention of cross-infections and monitoring of the efficacy of antimicrobial therapy. The aim of this study was to evaluate the antimicrobial resistance and prevalence of Pseudomonas aeruginosa serogroups isolated at Kaunas University of Medicine Hospital, Lithuania. Material and methods. Minimum inhibitory concentrations of piperacillin, cefoperazone, ceftazidime, cefotaxime, cefepime, imipenem, meropenem, gentamicin, amikacin, tobramycin, and ciprofloxacin for 609 Pseudomonas aeruginosa strains isolated from various clinical specimens between November 2001 and November 2002 were determined by the microdilution method in Mueller–Hinton agar using interpretative guidelines of National Committee for Clinical Laboratory Standards. Serogroups of Pseudomonas aeruginosa strains were identified using serums of Seiken Co. Ltd (Tokyo, Japan), containing antibodies against antigens of Pseudomonas aeruginosa O-group. Results. Pseudomonas aeruginosa strains were the most sensitive to ceftazidime (78.9%), imipenem (73.6%), meropenem (70.9%) and the most resistant to gentamicin (54.1%) and ciprofloxacin (52.5%). Multidrug-resistant strains made up 9.85% of all Pseudomonas aeruginosa strains investigated. Multidrug-resistant Pseudomonas aeruginosa strains were 1.5–3.5 times more resistant to antibiotics compared to non-multidrug-resistant strains, except to amikacin: multidrug-resistant strains were more sensitive (81.7%) than non-multidrug-resistant Pseudomonas aeruginosa strains (61.0%). Pseudomonas aeruginosa serogroups O:E and O:B were the most common serogroups (34.7% and 29.0%, respectively) followed by serogroups O:I (11.4%) and O:A (10.1%). Pseudomonas aeruginosa serogroup O:E strains were the most prevalent among multidrug-resistant strains (48.3%). Conclusions. The results of our study show that serogroup O:E was the most prevalent serogroup of Pseudomonas aeruginosa in our hospital, and its resistance to antibiotics was the highest.

scholarly journals Antibiotic Sensitivity of Pathogens Causing Urinary Tract Infection

2013 ◽  
Vol 16 (1) ◽  
pp. 53-58 ◽  
Author(s):  
Taslima Akter ◽  
Zakaria Mia ◽  
Masum Shahriar
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Age Groups ◽  
Antibiotic Sensitivity ◽  
Pharmaceutical Journal ◽  
Reproductive Age ◽  
Mueller Hinton ◽  
Sensitivity Pattern ◽  
Mueller Hinton Agar

Diagnosis of urinary tract infection (UTI) causing pathogens with their sensitivity to different antibiotics was performed with a total of 96 samples from both male (n=31; 32.3%) and female (n=65; 67.7%) of different age groups. Out of 96 urine samples, 55 (57.3%) were found positive after culturing in MacConkey agar plates. The percent distribution of positive cases against collected samples was higher for female (67%) than male (32%). However, female and male at the reproductive age of 16-30 years were more susceptible to UTI. A total of 55 bacterial isolates were identified by conventional methods and their antibiotic sensitivity was tested using Mueller- Hinton agar plates. The predominant isolates were Escherichia coli (34.5%), Klebsiella sp. (18.2%) and Staphylococci (20.0%). The sensitivity pattern for most of the isolated organisms showed 50% and/or higher sensitivity to imipenem, azithromycin and cephalexin, except Staphylococci (only 9.09% to azithromycin). DOI: http://dx.doi.org/10.3329/bpj.v16i1.14491 Bangladesh Pharmaceutical Journal 16(1): 53-58, 2013

scholarly journals Drug Susceptibility Testing and Synergistic Antibacterial Activity of Curcumin with Antibiotics against Enterotoxigenic Escherichia coli

Antibiotics ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 43 ◽  
Author(s):  
Rangel-Castañeda Itzia Azucena ◽  
Cruz-Lozano José Roberto ◽  
Zermeño-Ruiz Martin ◽  
Cortes-Zarate Rafael ◽  
Hernández-Hernández Leonardo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Clavulanic Acid ◽  
Susceptibility Testing ◽  
Drug Susceptibility ◽  
Drug Susceptibility Testing ◽  
Enterotoxigenic Escherichia Coli ◽  
Antimicrobial Drugs ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
Amoxicillin Clavulanic Acid

Aim: This study investigated the susceptibility of Enterotoxigenic Escherichia coli to curcumin, as well as its synergistic effect with 12 antimicrobial drugs. Methods and Results: Our study shows that curcumin did not affect bacterial growth. The antimicrobial susceptibility of curcumin and antibiotic synergy were identified using disc diffusion on Mueller-Hinton agar. The strain of Enterotoxigenic Escherichia coli used was resistant to Ampicillin, Amoxicillin/Clavulanic acid, Ampicillin/Sulbactam, Ciprofloxacin, and Cefazolin. There was synergy between curcumin and the majority of antibiotics tested. Maximum synergy was observed with combinations of 330 µg/mL curcumin and Ceftazidime, followed by Cefotaxime, Amoxicillin/Clavulanic acid, Ampicillin, Aztreonam, Trimethoprim, Ciprofloxacin, Ceftriaxone, Cefazolin, Tetracycline, and Imipenem. Conclusion: Our findings indicated that curcumin might be useful as a combinatorial strategy to combat the antibiotic resistance of Enterotoxigenic Escherichia coli.

scholarly journals Frequency and Antimicrobial Susceptibility Pattern of Pseudomonas Aeruginosa in Human Pus Samples at Holy Family Hospital Rawalpindi

2021 ◽  
Vol 6 (1) ◽  
pp. 1-8
Author(s):  
Kazmi A
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Nosocomial Infections ◽  
Antibiotic Susceptibility ◽  
Antimicrobial Agents ◽  
Antibiotic Sensitivity ◽  
Recovery Process ◽  
Multidrug Resistant ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Resistant Strains

Background: Nosocomial infections are great threat for hospitalized patients and Pseudomonas aeruginosa has emerged as one of the most potent nosocomial pathogens along with its diverse mechanisms to counter the various antimicrobial agents such as aminoglycosides, fluoroquinolones, monobactems, third generation cephalosporins, carbapenams and broad- spectrum penicillins. P. aeruginosa is one of the well-known pyogenic bacteria and is 3rd leading cause of pyogenic infections with the variable frequency depending on geographical region and clinical setting. P. aeruginosa is intimately associated with pyogenic nosocomial infections. Objectives: Since multidrug resistant strains of P. aeruginosa have posed serious threats and are frequently implicated in nosocomial infections. Methods: Pus swab were sampled under aseptic conditions and cultured on blood and Muller Hinton agar. Gram reaction, pigment production, Oxidase, indole reaction and citrate test were used to confirm isolate. Antibiotic susceptibility was performed b Kirby Bauer technique. Results compiled by us in this cross sectional study, showed 58 cases of P. aeruginosa out of 289 cases. This included 43% males and 57% females. Majority of the patients were of young age, with mean age 38 years. Antibiotic sensitivity revealed resistance to gentamicin was 50%, amikacin was 64%, ciprofloxacin and Aztronem 66%, Cefaparazone 69%, Tzaocin 71% and meropenem and sulzone was 79%. While Colistin and Ceftazidime were the most effective in 85% and 89% of cases respectively. The multidrug resistant strains of P. aeruginosa infections accounted for 32.76% of total P. aeruginosa infections. This study reveals high prevalence of multidrug resistant organisms at the set of our study. Based on this study, we suggest adopting the strategies to minimize the risk of nosocomial infections to slow down the rapidly growing multidrug resistance. These strategies may include, stricter antiseptic measures, fastening the recovery process and reducing the hospital stay and considering other alternates. Besides this, we would like to suggest the precise use of antibiotic susceptibility facility to reduce the nosocomial infection associated complications.

scholarly journals 656. Sulbactam-Durlobactam MIC Determination: Comparative Evaluation of the New ETEST® SUD to the CLSI 2021 Broth Microdilution Method

2021 ◽  
Vol 8 (Supplement_1) ◽  
pp. S430-S430
Author(s):  
Veronique Sauvonnet ◽  
Elodie Escoffier ◽  
Christine Franceschi ◽  
Diane Halimi ◽  
Roland Martelin ◽  
...  
Keyword(s):  
Reference Method ◽  
Multidrug Resistant ◽  
Error Rates ◽  
Broth Microdilution ◽  
Microdilution Method ◽  
Mueller Hinton ◽  
Broth Microdilution Method ◽  
Lactam Antibiotic ◽  
Visible Growth ◽  
Resistant Strains

Abstract Background Species belonging to the Acinetobacter baumannii-calcoaceticus (ABC) complex, such as A. baumannii, A. pittii and A. nosocomialis, are a major cause of hospital acquired infections and outbreaks with increasing occurrence of multidrug-resistance. Sulbactam-durlobactam (SUD), a combination of one active β-lactam antibiotic (sulbactam) with a new β-lactamase inhibitor (durlobactam), is currently being tested in a phase 3 clinical trial by Entasis Therapeutics for the treatment of serious infections caused by ABC, including multidrug-resistant strains. At the same time, an ETEST® SUD (sulbactam-durlobactam - MIC range 0.004/4-64/4 µg/mL) has been developed and calibrated versus the broth microdilution reference method (BMD) as described by the Clinical and Laboratory Standards Institute (CLSI). This test is intended to determine the MIC of sulbactam-durlobactam for species of the ABC complex. The aim of this study was to perform a first comparative study of ETEST SUD with the CLSI BMD method on a panel of 263 isolates. Methods The panel consisted of 204 A. baumannii, 29 A. pittii, 30 A. nosocomialis, including 24 SUD-resistant strains, and one CLSI QC strain. BMD was performed using the 2021 CLSI guidelines. ETEST SUD was evaluated using the standard ETEST procedure for Acinetobacter spp. (inoculum 0.5 McFarland, Mueller Hinton medium, incubation at 35°C for 20-24h). For each method, the MIC was read at complete inhibition of visible growth. To determine category agreement (CA) and error rates, the sulbactam-durlobactam provisional breakpoint of 4 µg/mL was applied. Results The QC strain MICs were in the expected range with reproducible results. The essential MIC agreement [EA, ±1 dilution] was 97.7% without any tendency to over- or underestimate the MIC when compared to BMD. The CA was 98.5%. Two Very Major Errors, both within the EA, and two Major Errors, one within the EA, were observed. Conclusion In this study, the ETEST SUD was found to be equivalent to the CLSI reference method. MIC end points were easy to read. With a 15-dilution range and simplicity of use, ETEST SUD could represent a valuable tool for MIC determination and could be an alternative to BMD. For Research Use Only. The performance characteristics of this product have not been established yet. Disclosures All Authors: No reported disclosures

scholarly journals Emergence of high multidrug-resistant Escherichia coli isolates from neonates with infection

2020 ◽  
Author(s):  
Dan Wu ◽  
Yijun Ding ◽  
Jinjing Zhang ◽  
Kaihu Yao ◽  
Wei Gao ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistance ◽  
Clavulanic Acid ◽  
Antimicrobial Agents ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
E Coli ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
Amoxicillin Clavulanic Acid

Abstract Background Escherichia coli (E.coli) rank one of the most common pathogens that can cause neonatal infections. The emergence of antibiotic-resistant bacteria is a major cause of treatment failure in newborn with infection. The purpose of this study was to describe the antibiotic resistance and multidrug-resistance of E.coli isolated from neonates with infection.Methods The antimicrobial susceptibility testing of the E. coli strains to selected antibiotics was assessed with the E-test technique on the Mueller-Hinton agar. The antimicrobials tests were included ceftazidime, cefuroxime, cefatriaxone, amoxicillin, amoxicillin-clavulanic acid, cefoperazone - sulbactam, meropenem, gentamicin, ciprofloxacin and sulfonamides. The minimal inhibitory concerntration (MIC) values of the antimicrobial agents selected for this study was determined by an agar dilution technique on Mueller-Hinton agar according to the Clinical and Laboratory Standards Institute recommendations. Results A total of 100 E. coli strains was isolated from phlegm (n = 78), blood (n = 10), cerebrospinal fluid (n = 5), and umbilical discharge (n = 7) of neonates hospitalized at Beijing Children’s Hospital. The highest resistance rate of E.coli was found in amoxicillin at 85%, followed by cefuroxime 65%, and cefatriaxone 60%, respectively. 6% and 5% of all isolates were resistant to amoxicillin/clavulanic acid and cefoperazone -sulbactam merely. The resistance rates to ceftazidime, gentamicin, ciprofloxacin and sulfonamides were 31%, 20%, 33%, 47%, respectively. All the isolates were susceptible to meropenem. Multidrug resistance was defined in E.coli as resistance to at least three antibiotic families. About 26% (26/100) of all the E.coli isolates were multidrug-resistant. The detection rate of ESBL-Producing E. coli was 55%. The rate in E. coli isolates from phlegm was higher than aseptic humoral. The difference was statistically significant (P < 0.05). It is worth noting that the majority of the isolates were also resistant to non-β-lactam antimicrobial agents, but the resistant rates were significantly lower than extended-spectrum β-lactamases.Conclusions: Multi-drug-resistant E.coli has become a thorny problem in clinical treatment. It is necessary to monitor E. coli resistance.

scholarly journals Testing the efficacy of Mueller Hinton agar over Nutrient agar for optimal antibiotic sensitivity testing response by selected clinical bacterial pathogens

2020 ◽  
Vol 5 (2) ◽  
pp. 061-074
Author(s):  
Otajevwo Dafinone Festus ◽  
Osawaru Osama Emmanuella
Keyword(s):  
Clavulanic Acid ◽  
Bacterial Pathogens ◽  
Antibiotic Sensitivity ◽  
Nutrient Agar ◽  
Diffusion Method ◽  
Sensitivity Testing ◽  
Mueller Hinton ◽  
Zones Of Inhibition ◽  
Mueller Hinton Agar ◽  
Amoxicillin Clavulanic Acid

The efficacy of Mueller Hinton agar over Nutrient agar in terms of antibiotic sensitivity testing for optimal antibiotic response by selected clinical bacterial pathogens was carried out in this study. Clinical bacterial pathogens used for the study were Pseudomonas aerµginosa, Enterococcus spp, Escherichia coli and Klebsiella pneumoniae. Standard and locally manufactured antibiotic discs used were by Abtek Biologicals Ltd, Liverpool and Maxicare Medical Laboratory, Nigeria respectively. Antibiotic sensitivity testing (AST) was by agar diffusion method. Pure cultures of each isolate were subcultured on sterile Mueller Hinton agar (MHA) and Nutrient agar (NA) media after which the standard and locally manufactured discs were aseptically impregnated on the media. All inoculated plates were incubated at 37oC for 24hrs aerobically after appropriate labeling. Zones of inhibition were measured by standard methods and recorded. On Nutrient agar, standard and locally produced ciprofloxacin, ofloxacin, gentamycin and amoxicillin/clavulanic acid discs did not produce zones of inhibition significantly different from each other at both 95% and 99% confidence intervals (P ˃ 0.05 and P ˃ 0.01). On Mueller Hinton agar, standard and locally manufactured ciprofloxacin, ofloxacin, gentamycin and amoxicillin/clavulanic acid discs produced zones of inhibition that were significantly different from each other at 95% confidence interval (P ˂ 0.05). Standard and local ciprofloxacin, ofloxacin, gentamycin and amoxicillin/clavulanic acid discs produced zones of inhibition on MHA and NA which were not significantly different (P ˃ 0.05 and P ˃ 0.01). Standard discs used recorded better zones of inhibition on MHA compared to the local discs. Standard and local discs zones of inhibition on MHA was however not significantly different from those recorded on NA (P ˃ 0.05). Standard discs therefore, did not produce better zones of inhibition over local discs on MHA and on NA. On the whole, the use of MHA for antibiotic sensitivity testing did not record greater (better) zones of inhibition than those recorded on NA except for standard ciprofloxacin, ofloxacin and gentamycin discs over the corresponding local discs on MHA only. Findings did not convincingly establish better performance of standard discs over local discs whether used on MHA or NA. Further studies in this direction is recommended.

scholarly journals Chlamydophila psittaci in homing and feral pigeons and zoonotic transmission

2010 ◽  
Vol 59 (11) ◽  
pp. 1348-1353 ◽  
Author(s):  
V. Dickx ◽  
D. S. A. Beeckman ◽  
L. Dossche ◽  
P. Tavernier ◽  
D. Vanrompay
Keyword(s):  
Protein A ◽  
Homing Pigeon ◽  
Zoonotic Transmission ◽  
Antimicrobial Drugs ◽  
Homing Pigeons ◽  
Feral Pigeons ◽  
Chlamydophila Psittaci ◽  
Resistant Strains ◽  
Drug Resistant Strains ◽  
The City

Chlamydiosis is a zoonotic disease in birds caused by Chlamydophila psittaci, an obligate intracellular bacterium. There are seven known avian outer-membrane protein A genotypes, A–F and E/B. The importance of genotyping lies in the fact that certain genotypes tend to be associated with certain hosts and a difference in virulence. Genotype B is the most prevalent in pigeons, but the more virulent genotypes A and D have also been discovered. The current study assessed the prevalence of C. psittaci in 32 Belgian homing-pigeon facilities and in 61 feral pigeons captured in the city of Ghent, Belgium. Additionally, zoonotic transmission of C. psittaci was investigated in the homing-pigeon facilities. Homing pigeons were often infected, as at least one of the lofts was positive in 13 of the 32 (40.6 %) pigeon breeding facilities. Genotypes B, C and D were detected. Zoonotic transmission was discovered in 4 of the 32 (12.5 %) pigeon fanciers, revealing genotype D in two of them, whilst genotyping was unsuccessful for the other two human pharyngeal swabs. This study clearly demonstrates the possible risk of C. psittaci zoonotic transmission from homing pigeons. Pigeon fanciers often (37.5 %) used antibiotics for prevention of respiratory disease. Because of the risk of developing drug-resistant strains, regular use of antimicrobial drugs must be avoided. This study is believed to be the first to detect C. psittaci in Belgian feral pigeons. The prevalence rate in the city of Ghent was extremely low, which is beneficial for public health.

scholarly journals Inducible clindamycin resistance among staphylococci isolated from clinical samples in an urban hospital of Dhaka City

1970 ◽  
Vol 5 (1) ◽  
pp. 6-8
Author(s):  
Shameem Akhter ◽  
SM Zahurul Haque ◽  
M Mushfequr Rahman
Keyword(s):  
Staphylococcus Aureus ◽  
Tertiary Care ◽  
Clinical Samples ◽  
Care Hospital ◽  
Urban Hospital ◽  
Inducible Clindamycin Resistance ◽  
Inducible Resistance ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
Resistant Strains

Inducible clindamycin resistance was detemined in 200 clinical isolates of staphylococci from pus (53.5%) and wound swab (46.5%). The study was done from July 2009 to June 2010, in the Department of Microbiology, BIHS Hospital Dhaka. Inducible clindamycin resistance was demonstrated by placing an erythromycin disc (15 ìg) 15 mm apart from the edge of a clindamycin (2 ìg) disc in Mueller Hinton agar. When the clindamycin inhibited zone becomes D- shaped the organism was regarded as positive for inducible resistance (D- test positive). Out of 200 staphylococci, 20% had inducible clindamycin resistance, 5% had constitutive clindamycin resistance and remaining 75% was clindamycin sensitive. In case of methicillin resistant Staphylococcus aureus (MRSA), 48% had inducible clindamycin resistance while 11.5% was constitutively resistant to clindamycin and remainder were clindamycin sensitive. All clindamycin resistant strains were 100% sensitive to vancomycin and linezolid followed by gentamycin (42%) and tetracycline (42.3%). The findings demonstrated that a substantial proportion of staphylococci in our tertiary care hospital had inducible resistance to clindamycin.Ibrahim Med. Coll. J. 2011; 5(1): 6-8 Key words: Staphylococcus aureus; Inducible clindamycin resistance; Constitutive clindamycin resistance; D-testDOI: http://dx.doi.org/10.3329/imcj.v5i1.9853  

Sign in / Sign up

Export Citation Format

Share Document