scholarly journals Perubahan kadar kolesterol total dan trigliserida pada kelinci New Zealand white yang diberi ekstrak beras hitam (Oriza sativa L.)

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Lastry Glory ◽  
Shirley E. Kawengian ◽  
Nelly Mayulu
Keyword(s):  
Oryza Sativa ◽  
New Zealand ◽  
Total Cholesterol ◽  
High Fat Diet ◽  
Oryza Sativa L ◽  
White Male ◽  
Control Group ◽  
Black Rice ◽  
High Fat ◽  
Standard Meal

Abstract: High cholesterol levels in blood can lead to various diseases, including coronary heart disease. Black rice (Oryza sativa L.) contains more anthocyanin in layers of alueron than in white rice. Cardioprotective effect of rice has been emerged since several decades ago. This was a true experimental study with a pre-posttest control group design. Samples were New Zealand White male rabbits aged 3 months. Group 1 was fed standard meal; group 2 was fed with high-fat diet; group 3 was fed with high-fat diet plus black rice extract; and group 4 were fed with high-fat diet plus atorvastatin. Data were analyzed and presented descriptively. The results showed that total cholesterol and triglyceride levels in the group fed with black rice extract decreased by 5 mg/dl and 4 mg/dl respectively. Decreased total cholesterol and triglyceride levels were also found in the group fed with standard meal and the group given atorvastatin. Meanwhile, the group fed with high-fat diet showed increased levels of total cholesterol and triglyceride high enough. Conclusion: There were decreased levels of total cholesterol and triglycerides in rabbits fed with black rice extract. Keywords: black rice extract, total cholesterol, triglycerides. Abstrak: Kadar kolesterol yang tinggi dalam darah dapat memicu berbagai macam penyakit, diantaranya penyakit jantung koroner. Beras hitam (Oryza sativa L.) mengandung lebih banyak antosianin dalam lapisan alueronnya dibandingkan beras putih. Efek kardioprotektif dari beras telah dikemukakan sejak beberapa dekade yang lalu. Jenis penelitian ini true experimental dengan pre test post test control grup design. Sampel ialah kelinci New Zealand White jantan berusia 3 bulan. Kelompok perlakuan 1 diberi pakan standar; kelompok 2 diberi diet tinggi lemak; kelompok 3 diberi diet tinggi lemak dan ekstrak beras hitam dan kelompok 4 diberi diet tinggi lemak dan atorvastatin. Data dianalisis dan disajikan secara deskriptif. Hasil penelitian memperlihatkan kadar kolesterol total dan trigliserida pada kelompok yang diberi ekstrak beras hitam mengalami penurunan masing-masing sebesar 5 mg/dl dan 4 mg/dl. Penurunan kadar kolesterol dan trigliserida juga terdapat pada kelompok yang diberi pakan standar dan kelompok yang diberi atorvastatin. Pada kelompok yang diberi diet tinggi lemak terjadi peningkatan kadar kolesterol total dan trigliserida yang cukup besar. Simpulan: Terjadi penurunan kadar kolesterol total dan trigliserida pada kelinci yang diberi ekstrak beras hitam.Kata kunci: ekstrak beras hitam, kolesterol total, trigliserid.

scholarly journals Gambaran histologik aorta kelinci yang diinduksi dengan lemak babi dan diberi ekstrak beras hitam

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Lendy S.F. Polii ◽  
Djon Wongkar ◽  
Sunny Wangko
Keyword(s):  
Oryza Sativa ◽  
New Zealand ◽  
High Fat Diet ◽  
Oryza Sativa L ◽  
Foam Cells ◽  
Black Rice ◽  
High Fat ◽  
Cholesterol Levels ◽  
Post Test ◽  
Group A

Abstract: Dyslipidemia is an abnormal lipid metabolism, marked by the alteration of lipid fractions within the blood plasma. World Health Organization reported that dyslipidemia was associated with heart diseases in general with 4 million deaths each year. Dyslipidemia is mostly caused by unhealthy lifestyle. The change of lifestyle can be initiated by the modification of diet. Black rice (Oryza sativa L.) is a type of local rice which contains a different pigment than the regular or any other colored rice. This study aimed to identify the histological changes of rabbit aorta induced with lard and black rice extract diet. This was an experimental study with a post-test design. Subjects were three rabbits New Zealand white divided into 3 groups (A. B. And C) of 1 rabbit each. Group A was fed with standard diet (Vitamax), group B with high fat diet, meanwhile group C was fed with high fat diet added with black rice extracts. This study was carried out for 28 days, after that all the rabbits were terminated to obtain their aortas. The results showed that there were reductions of LDL and total cholesterol levels of group C. Foam cells were found in the tunica intima and tunica media in group A, B and C. However, the foam cells in group C were less than the other groups. Conclusion: Black rice diet could reduce the LDL and total cholesterol levels as well as the number of foam cells in the aorta tunica of rabbits. Keywords: aorta, rabbits, foam cells, black rice extracts, lard Abstrak: Dislipidemia adalah kelainan metabolisme lipid yang ditandai dengan peningkatan maupun penurunan fraksi lipid dalam plasma. WHO memperkirakan dislipidemia berhubungan dengan kasus penyakit jantung secara luas, serta menyebabkan empat juta kematian per tahun. Dislipidemia umumnya disebabkan karena gaya hidup. Perubahan gaya hidup dapat dilakukan mulai dari memodifikasi pola diet. Beras hitam (Oryza sativa L.) merupakan varietas lokal yang mengandung pigmen berbeda dengan beras putih atau beras berwarna lain. Penelitian ini bertujuan untuk mendapatkan gambaran histologik aorta kelinci yang diinduksi dengan lemak babi dan diberi pakan beras hitam. Jenis penelitian ini eksperimental dengan rancangan post test. Subyek penelitian kelinci New Zealand white dibagi menjadi tiga kelompok (A, B, dan C), masing-masing kelompok terdiri dari 1 kelinci. Kelompok A diberikan pakan standar (Vitamax), kelompok B diberikan diet tinggi lemak, dan kelompok C diberikan diet tinggi lemak serta ekstrak beras hitam. Perlakuan diberikan selama 28 hari dan selanjutnya dilakukan terminasi untuk mengambil aorta kelinci dan kemudian dianalisis. Hasil penelitian memperlihatkan penurunan kadar kolesterol LDL dan total pada kelompok C. Gambaran histologik aorta kelompok A, B dan C menunjukkan adanya sel-sel busa di lapisan tunika intima dan tunika media, namun jumlah sel busa di kelompok C lebih sedikit dari kelompok yang lain. Simpulan: Ekstrak beras hitam dapat menurunkan kadar kolesterol LDL dan total, serta jumlah sel busa di lapisan aorta pada kelinci.Kata kunci: aorta, kelinci, sel busa, ekstrak beras hitam, lemak babi

scholarly journals Perubahan kadar LDL dan HDL pada kelinci New Zealand White yang diberi ekstrak beras hitam (Oryza sativa L.)

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Jessica G. Runtu ◽  
Shirley E.S. Kawengian ◽  
Nelly Mayulu ◽  
Alexander S.L. Bolang
Keyword(s):  
Oryza Sativa ◽  
New Zealand ◽  
High Fat Diet ◽  
Oryza Sativa L ◽  
Diet Group ◽  
Standard Diet ◽  
Black Rice ◽  
High Fat ◽  
Increased Risk ◽  
The Mean

Abstract: Cardiovascular disease, especially coronary heart disease (CHD), is the leading cause of death annually. Low HDL and high LDL levels are related to the increased risk of CHD. Polyphenol such as anthocyanin in black rice could reduce the cardiovascular risks due to its antioxidant that has anti-platelet and anti-inflammatory activities. This study was aimed to determine the changes in LDL and HDL levels in rabbits fed with black rice extract (Oryza sativa L.). This was a true experimental study with a pretest posttest control group design. Samples were male New Zealand White rabbits, weighing 0.8-2.5 kg divided into four groups. Group A was fed with standard diet; group B was fed with high-fat diet; group C was fed with high-fat diet and black rice extract; and group D was fed with high-fat diet and atorvastatin. Data were presented descriptively. The results showed that the mean LDL level of group C increased by 6.5 mg/dl but one sample showed a decrease of 2 mg/dl. The mean level of HDL of group C decreased 0.50 mg/dl. This reduction was not as many as the other groups but one sample showed an increase of 5 mg/dl. Conclusion: Not all rabbits fed with black rice extract showed decreased LDL level and increased HDL level.Keywords: LDL, HDL, black rice extract Abstrak: Penyakit kardiovaskuler terutama penyakit jantung koroner (PJK) merupakan penyebab kematian tertinggi pertahunnya. Kadar HDL rendah serta kadar LDL tinggi berkaitan dengan meningkatnya risiko PJK. Polifenol seperti antosianin yang terdapat dalam beras hitam dapat menurunkan risiko penyakit kardiovaskular karena antioksidannya dengan aktivitas anti-platelet dan anti-inflamasi. Penelitian ini bertujuan untuk mengetahui perubahan kadar LDL dan HDL pada kelinci yang diberi ekstrak beras hitam (Oryza sativa L.). Jenis penelitian ialah true experimental dengan desain pre test post test control grup. Sampel ialah kelinci New Zealand White jantan, berat badan 0,8- 2,5 kg. Kelompok A diberi pakan standar; kelompok B diberi diet tinggi lemak; kelompok C diberi diet tinggi lemak dan ekstrak beras hitam; dan kelompok D diberi diet tinggi lemak dan atorvastatin. Data disajikan secara deskriptif. Hasil penelitian mendapatkan pada kelompok C, rerata kadar LDL meningkat 6,5 mg/dl tetapi satu sampel mengalami penurunan sebesar 2 mg/dl. Rerata kadar HDL pada kelompok C menurun 0,50 mg/dl; penurunan ini tidak sebesar pada kelompok lainnya tetapi satu sampel mengalami peningkatan sebesar 5 mg/dl. Simpulan: Kelinci yang diberi ekstrak beras hitam tidak semua mengalami penurunan kadar LDL dan peningkatan kadar HDL. Kata kunci: LDL, HDL, Ekstrak beras hitam

scholarly journals Black rice (Oryza sativa L.) extract attenuates hepatic steatosis in C57BL/6 J mice fed a high-fat diet via fatty acid oxidation

2012 ◽  
Vol 9 (1) ◽  
pp. 27 ◽  
Author(s):  
Hwan-Hee Jang ◽  
Mi-Young Park ◽  
Heon-Woong Kim ◽  
Young-Min Lee ◽  
Kyung-A Hwang ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Fatty Acid ◽  
Hepatic Steatosis ◽  
Fatty Acid Oxidation ◽  
High Fat Diet ◽  
Oryza Sativa L ◽  
Acid Oxidation ◽  
Black Rice ◽  
High Fat

scholarly journals Black rice (Oryza sativa L.) reduces obesity and improves lipid metabolism in C57BL/6J mice fed a high-fat diet

2020 ◽  
Vol 64 ◽  
pp. 103605 ◽  
Author(s):  
Dong Liu ◽  
Yanglin Ji ◽  
Jiang Zhao ◽  
Huali Wang ◽  
Yatu Guo ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Lipid Metabolism ◽  
High Fat Diet ◽  
Oryza Sativa L ◽  
Black Rice ◽  
High Fat

scholarly journals ANTIHYPERLIPIDEMIC EFFECT OF THE ETHANOL EXTRACT FRACTION FROM MULBERRY (MORUS AUSTRALIS POIR.) LEAVES ON RATS INDUCED HIGH FAT-DIET (HFD) AND PROPYLTHIOURACIL (PTU)

2021 ◽  
pp. 157-161
Author(s):  
PRISKA ERNESTINA TENDA ◽  
ELEONORA MARYETA TOYO ◽  
ARBA PRAMUNDITA RAMADANI
Keyword(s):  
Ethyl Acetate ◽  
Total Cholesterol ◽  
High Fat Diet ◽  
Cholesterol Oxidase ◽  
Ethyl Acetate Fraction ◽  
Control Group ◽  
High Cholesterol Diet ◽  
High Fat ◽  
Control Group Design ◽  
Mulberry Leaf

Objectives: To evaluate the effect of extract and its fraction (n-hexane, ethyl acetate, and water) of the mulberry leaf (Morus australis Poir.) in reducing triglyceride and total cholesterol levels on HFD and PTU induced rats. Methods: In this study, mulberry leaves were collected from East Nusa Tenggara-Indonesia, extracted using maceration method, and fractionated with various solvents. The thin layer chromatography with silica gel 60 F254 was utilized to identify the active compound on both extract and fraction. Furthermore, using a quasi-experimental with control group design, this study employed 40 healthy male Wistar rats and divided into 8 groups, namely: normal and negative control, positive controls (simvastatin and gemfibrozil), ethanolic extract, and fractions (n-hexane, ethyl acetate, and water). All groups were fed with a high cholesterol diet and 0.01% PTU, except for the normal group. During the study, the blood was drawn at day 0, 28, 35, and 42. The triglyceride level was observed using Glycerophosphate-Oxidase Phenol Aminoantipyrine (GPO-PAP) method, while the total cholesterol was measured by Cholesterol Oxidase Phenol Aminoantipyrine (CHOD-PAP). Results: On the 42nd day, ethyl fraction of mulberry leaf (Morus australis Poir.) showed the most significant result of reducing the level of triglyceride and total cholesterol compared to the high-fat diet and PTU induced rat on day 0: triglyceride of 104.89±2.70 mg/dl vs 64.76±1.97 mg/dl and total cholesterol of 96.70±2.45 vs 88.02±1.38 mg/dl. Ethyl acetate fraction was similar to simvastatin in lowering the level of total cholesterol. TLC identification also showed that mulberry leaf contains flavonoids and polyphenols that act as anti-hyperlipidemic. Conclusion: Ethyl acetate fraction of mulberry leaf (Morus australis Poir.) showed best activity on lowering both triglyceride and total cholesterol.

scholarly journals Gambaran histologik hati pada kelinci yang diinduksi lemak dengan pemberian ekstrak beras hitam

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Yosua R. Somba ◽  
Djon Wongkar ◽  
Shane H. R. Ticoalu ◽  
Alexander S. L. Bolang
Keyword(s):  
Reactive Oxygen Species ◽  
Oryza Sativa ◽  
Ldl Cholesterol ◽  
Oryza Sativa L ◽  
Control Group ◽  
Oxygen Species ◽  
Black Rice ◽  
Control Group Design ◽  
Group Design ◽  
Signet Ring

Abstract: Dyslipidemia is a disorder of fat metabolism which is marked by increase of one or more fractions of fat in the blood. Due to the high rate of dyslipidemia cases, people begin to improve their lifestyle, such as consuming black rice. Black rice (Oryza sativa L.) is a local variety of rice that contains pigments which are different from other types of rice. Black rice extract is beneficial for lowering LDL cholesterol level and significantly increasing HDL cholesterol level besides its antioxidant effect against reactive oxygen species. This was an experimental posttest-only control group design. Subjects were three New Zealand White rabbits, divided into three groups. Group A was fed with standard food for rabbit; group B was fed with standard food for rabbit and fat; group C was fed with standard foot for rabbit, fat, and black rice extract for 28 days. Blood total and LDL cholesterol were examined on the first day and after 28 days of treatment. The three groups were terminated and their liver tissues were processed for histological examination. The histological results were as follows: Group one showed small fat vacuoles around the hepatocyte nuclei; group two showed large fat vacuoles around the hepatocyte nuclei, signet rings, and coalescence of fat vacuoles; group three showed fat vacuoles around the hepatocyte nuclei, a few signet rings without coalescence of fat vacuoles. Conclusion: Black rice extract could reduce fat vacuoles in liver tissue.Keywords: fat, black rice extract, dyslipidemia, fatty liverAbstrak: Dislipidemia adalah kelainan metabolisme lemak yang ditandai peningkatan salah satu atau lebih dari fraksi lemak di dalam darah. Meningkatnya kasus dislipidemia menyebabkan banyak orang beralih ke gaya hidup sehat, antara lain mengonsumsi beras hitam. Beras hitam (Oryza sativa L.) merupakan beras varietas lokal dengan pigmen yang berbeda dari beras lain oleh karena kandungan antosianin. Ekstrak beras hitam bermanfaat untuk menurunkan kadar kolesterol total dan LDL secara bermakna, meningkatkan kadar kolesterol HDL, serta memiliki efek antioksidan terhadap reactive oxygen species. Jenis penelitian ini ialah experimental posttest-only control group design. Sampel penelitian ini ialah kelinci New Zealand White sebanyak 3 ekor yang dibagi menjadi 3 kelompok. Kelompok A diberi pakan standar; kelompok B diberi pakan standar dan lemak; dan kelompok C diberi pakan standar dengan lemak dan ekstrak beras hitam selama 28 hari. Pemeriksaan kkolesterol total dan LDL dilakukan pada awal penelitian dan 28 hari setelah perlakuan 28 hari. Kelinci diterminasi untuk pengambilan jaringan hati yang kemudian dibuat sediaan histologik. Hasil pemeriksaan histologik ialah sebagai berikut: Kelompok 1 menunjukkan adanya vakuola lemak kecil-kecil disekitar nukleus; kelompok 2 menunjukkan adanya vakuola lemak yang luas di sekitar nukleus, gambaran signet ring, dan penggabungan vakuola lemak; kelompok 3 menunjukkan adanya vakuola lemak disekitar nukleus dengan beberapa gambaran signet ring tanpa penggabungan vakuola lemak. Simpulan: Ekstrak beras hitam dapat mengurangi vakuola lemak pada jaringan hatiKata kunci: lemak, ekstrak beras hitam, dislipidemia, perlemakan hati

scholarly journals Anti-hypercholesterolemic effect of Zingiber montanum extract

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 1798
Author(s):  
Swandari Paramita ◽  
Meiliati Aminyoto ◽  
Sjarif Ismail ◽  
Enos Tangke Arung
Keyword(s):  
Total Cholesterol ◽  
High Fat Diet ◽  
Diet Group ◽  
High Density Lipoproteins ◽  
Control Group ◽  
High Cholesterol ◽  
High Fat ◽  
Cholesterol Levels ◽  
White Rats ◽  
Diet Control

Background: Hypercholesterolemia, high cholesterol levels in the blood, can contribute to many forms of disease, most notably cardiovascular disease. Anti-hypercholesterolemic agents generally used for those conditions have several side effects for patients. Zingiber montanum, known locally as “bangle”, belongs to the family Zingiberaceae and is a potential plants for alternative anti-hypercholesterolemic agents. This plant, from East Kalimantan, is used in traditional medicine for health problems caused by high cholesterol levels. The aim of this research was to find alternatives to anti-hypercholesterolemic agents, especially from natural sources. Methods: This study was an experimental study using 30 Wistar male white rats. Subjects were randomly divided into 6 groups (n=5): (1) normal control group; (2) high fat diet control group; (3) high fat diet with simvastatin; (4-6) high fat diet with Zingiber montanum extracts 100, 200, and 400 mg/kg. After 4 weeks of treatment, blood was collected from all groups, and plasma concentrations of triglycerides, total cholesterol, high density lipoproteins (HDL), and low density lipoproteins (LDL) were measured. Results: The results showed significant differences in total cholesterol (p=0.000), LDL (p=0.000) and triglycerides (p=0.001) in the high-fat diet group with Z. montanum extract, as compared to the high-fat diet control. Meanwhile, there were no significant differences in HDL levels (p=0.830) between the high-fat diet group and other groups. The results also showed significant differences in total cholesterol and LDLs for rats treated with Z. montanum extract, 100 mg/kg (p=0.000), 200 mg/kg (p=0.000), and 400 mg/kg (p=0.000) compared to the high-fat diet group. The result of Z. montanum 400 mg/kg also showed a significant reduction, not only for total cholesterol and LDLs, but also for triglycerides (p=0.030). Conclusion: It could be concluded that Z. montanum extracts have the potency to be further developed as a new natural source of the anti-hypercholesterolemic agents.

scholarly journals Milk minerals modify the effect of fat intake on serum lipid profile: results from an animal and a human short-term study

2013 ◽  
Vol 111 (8) ◽  
pp. 1412-1420 ◽  
Author(s):  
Janne K. Lorenzen ◽  
Søren K. Jensen ◽  
Arne Astrup
Keyword(s):  
Total Cholesterol ◽  
High Fat Diet ◽  
Ldl Cholesterol ◽  
Control Period ◽  
Saturated Fat ◽  
Hdl Cholesterol ◽  
Cholesterol Concentration ◽  
Control Group ◽  
High Fat ◽  
Milk Minerals

Despite a high content of saturated fat, evidence from observational studies indicates that the consumption of dairy products may have a neutral effect or may be inversely associated with the risk of CVD. We aimed to examine whether milk minerals modify the effect of saturated fat on serum lipid profile. We present data from two studies. Study I had a randomised, blinded, parallel design (n 24 pigs) with a 10 d adaptation period during which a high-fat diet was fed to the pigs and a 14 d intervention period during which the same diet either enriched with milk minerals (MM group) or placebo (control group) was fed to the pigs. Study II had a randomised cross-over design (n 9 men) where the subjects were fed either a high-fat diet enriched with milk minerals (MM period) or a regular diet (control period). In both the studies, blood variables were measured before and after the intervention and faecal and urine samples were collected at the end of the dietary periods. The increase in plasma total cholesterol and LDL-cholesterol concentrations but not in HDL-cholesterol concentration was markedly lowered by milk minerals in both the studies. In the animal study, baseline adjusted total cholesterol and LDL-cholesterol concentrations in the MM group were 11 % (P= 0·004) and 13 % (P= 0·03) lower compared with those in the control group after the intervention. Similarly in the human study, baseline adjusted total cholesterol and LDL-cholesterol concentrations were 6 % (P= 0·002) and 9 % (P= 0·03) lower after the MM period compared with those in the control period. HDL-cholesterol concentration was not lowered by milk minerals. These short-term studies indicate that the addition of milk minerals to a high-fat diet to some extent attenuates the increase in total cholesterol and LDL-cholesterol concentrations, without affecting HDL-cholesterol concentration.

scholarly journals Nerium oleanderDistillate Improves Fat and Glucose Metabolism in High-Fat Diet-Fed Streptozotocin-Induced Diabetic Rats

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Ahmet Levent Bas ◽  
Sule Demirci ◽  
Nuray Yazihan ◽  
Kamil Uney ◽  
Ezgi Ermis Kaya
Keyword(s):  
Glucose Metabolism ◽  
Total Cholesterol ◽  
High Fat Diet ◽  
Atherogenic Index ◽  
Nerium Oleander ◽  
Control Group ◽  
Distilled Water ◽  
High Fat ◽  
Type 2 Diabetic

Diabetes was induced by intraperitoneal injection of streptozotocin (35 mg/kg bw) in all rats of five groups after being fed for 2 weeks high-fat diet. Type 2 diabeticNerium-oleander-(NO-) administered groups received the NO distillate at a dose of 3.75, 37.5, and 375 μg/0.5 mL of distilled water (NO-0.1, NO-1, NO-10, resp.); positive control group had 0.6 mg glibenclamide/kg bw/d by gavage daily for 12 weeks. Type 2 diabetic negative control group had no treatment. NO distillate administration reduced fasting blood glucose, HbA1c, insulin resistance, total cholesterol, low density lipoprotein, atherogenic index, triglyceride-HDL ratio, insulin, and leptin levels. Improved beta cell function and HDL concentration were observed by NO usage. HDL percentage in total cholesterol of all NO groups was similar to healthy control. NO-10 distillate enhanced mRNA expressions of peroxisome proliferator-activated-receptor- (PPAR-)α,β, andγin adipose tissue and PPAR-α–γin liver. The findings from bothin vivoandin vitrostudies suggest that the considerable beneficial effect of NO distillate administration at a dose of 375 μg/0.5 mL of distilled water may offer new approaches to treatment strategies that target both fat and glucose metabolism in type 2 diabetes.

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