scholarly journals Uji Daya Hambat Ekstrak Spons Laut Callyspongia aerizusa terhadap Pertumbuhan Bakteri Shigella dan Staphylococcus epidermidis

2017 ◽  
Vol 5 (2) ◽  
Author(s):  
Tifany T. Korompis ◽  
Christi D. Mambo ◽  
Edward Nangoy
Keyword(s):  
Secondary Metabolites ◽  
Laboratory Study ◽  
Marine Sponge ◽  
Staphylococcus Epidermidis ◽  
Antibacterial Agents ◽  
Marine Sponges ◽  
Inhibition Activity ◽  
Experimental Laboratory ◽  
Inhibition Zones ◽  
Sponge Extract

Abstract: Callyspongia sp. is a kind of marine sponges that produces secondary metabolites, such as steroids, alkaloids, flavonoids, and terpenoids which can be used as antibacterial agents. This study was aimed to determine the inhibition activity of Callyspongia aerizusa marine sponge extract against the growth of Shigella and Staphylococcus epidermidis bacteria. This was an experimental laboratory study. Inhibition activity was tested by using the modified Kirby-Bauer method. The inhibition zones formed by the marine sponge extract were measured. The results showed that the inhibition zones of marine sponge extract against Shigella bacteria was 6.1 mm and against Staphylococcus epidermidis bacteria was 6.6 mm. Conclusion: Callyspongia aerizusa extract had moderate inhibition activity against the growth of Shigella and Staphylococcus epidermidis bacteria.Keywords: inhibition test, Callyspongia aerizusa, Shigella, Staphylococcus epidermidis Abstrak: Callyspongia sp. merupakan salah satu jenis spons laut yang menghasilkan metabolit sekunder berupa steroid, alkaloid, flavonoid, dan terpenoid yang bisa dimanfaatkan sebagai bahan antibakteri. Penelitian ini bertujuan untuk mengetahui ada tidaknya daya hambat dari ekstrak spons laut Callyspongia aerizusa terhadap pertumbuhan bakteri Shigella dan Staphylococcus epidermidis. Jenis penelitian ialah eksperimental laboratorik. Uji daya hambat dengan metode Kirby-Bauer yang dimodifikasi menggunakan sumuran untuk mengukur zona hambat yang terbentuk oleh ekstrak spons laut Callyspongia aerizusa. Hasil penelitian mendapatkan zona hambat yang terbentuk pada bakteri Shigella sebesar 6,1 mm dan pada bakteri Staphylococcus epidermidis sebesar 6,6 mm. Simpulan: Ekstrak spons laut Callyspongia aerizusa memiliki daya hambat sedang terhadap pertumbuhan bakteri Shigella dan Staphylococcus epidermidis.Kata kunci: uji daya hambat, Callyspongia aerizusa, Shigella, Staphylococcus epidermidis

scholarly journals Identification of Cytotoxicity of Marine Sponge Dendrilla Membranosa Against L929 Cell Line

2019 ◽  
pp. 147-152
Author(s):  
Joicy Abraham ◽  
Mahija S. P ◽  
Jency George ◽  
Manjusha W. A
Keyword(s):  
Marine Sponge ◽  
Octanoic Acid ◽  
L929 Cell ◽  
Bioactive Compound ◽  
Marine Sponges ◽  
Test Material ◽  
Ms Analysis ◽  
Benzenedicarboxylic Acid ◽  
Sponge Extract ◽  
L929 Cell Line

Marine sponges are rich sources of pharmacological active compound. Marine sponge, Dendrilla membranosa was collected from the Vizhingam coast. The sponge extract was tested against eight human bacterial pathogens. The bioactive compounds present in marine sponge were determined by GC-MS analysis. The cytotoxic effect of the sponge was evaluated by using MTT assay. The extracts showed potent anti-bacterial activity against Staphylococcus aureus, Bacillus subtilis, Streptococcus pyogens. The bioactive compound present in marine sponge was identified by GC-MS analysis and the compounds are ethane, butane, formate pentane, Alpha d-galactopyranoside, 2-Ethylhexyl2-ethylhexanoate, Hexahydro hexitol, Styrene, Alpha-d glucopyranoside 1-pentyl-2-proppyl-1-octene, nonadecane, 1,2-benzenedicarboxylic acid, bis-2, Octanoic acid. The test material showed none cytotoxic response to fibroblasts cells. The results of present investigation revealed that, Dendrilla membranosa is a potential source of novel anticancer and antibacterial leads.

scholarly journals Microorganisms Associated with the Marine Sponge Scopalina hapalia: A Reservoir of Bioactive Molecules to Slow Down the Aging Process

2020 ◽  
Vol 8 (9) ◽  
pp. 1262
Author(s):  
Charifat Said Hassane ◽  
Mireille Fouillaud ◽  
Géraldine Le Goff ◽  
Aimilia D. Sklirou ◽  
Jean Bernard Boyer ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Marine Sponge ◽  
Marine Sponges ◽  
Sirtuin 1 ◽  
Bioactive Molecules ◽  
Aging Research ◽  
Fyn Kinase ◽  
The Indian Ocean ◽  
Metabolites Production ◽  
Future Work

Aging research aims at developing interventions that delay normal aging processes and some related pathologies. Recently, many compounds and extracts from natural products have been shown to delay aging and/or extend lifespan. Marine sponges and their associated microorganisms have been found to produce a wide variety of bioactive secondary metabolites; however, those from the Southwest of the Indian Ocean are much less studied, especially regarding anti-aging activities. In this study, the microbial diversity of the marine sponge Scopalina hapalia was investigated by metagenomic analysis. Twenty-six bacterial and two archaeal phyla were recovered from the sponge, of which the Proteobacteria phylum was the most abundant. In addition, thirty isolates from S. hapalia were selected and cultivated for identification and secondary metabolites production. The selected isolates were affiliated to the genera Bacillus, Micromonospora, Rhodoccocus, Salinispora, Aspergillus, Chaetomium, Nigrospora and unidentified genera related to the family Thermoactinomycetaceae. Crude extracts from selected microbial cultures were found to be active against seven targets i.e., elastase, tyrosinase, catalase, sirtuin 1, Cyclin-dependent kinase 7 (CDK7), Fyn kinase and proteasome. These results highlight the potential of microorganisms associated with a marine sponge from Mayotte to produce anti-aging compounds. Future work will focus on the isolation and the characterization of bioactive molecules.

scholarly journals Biological and Chemical Diversity of Marine Sponge-Derived Microorganisms over the Last Two Decades from 1998 to 2017

Molecules ◽  
2020 ◽  
Vol 25 (4) ◽  
pp. 853 ◽  
Author(s):  
Mei-Mei Cheng ◽  
Xu-Li Tang ◽  
Yan-Ting Sun ◽  
Dong-Yang Song ◽  
Yu-Jing Cheng ◽  
...  
Keyword(s):  
Natural Products ◽  
Secondary Metabolites ◽  
Natural Product ◽  
Marine Sponge ◽  
Marine Sponges ◽  
Chemical Diversity ◽  
Comprehensive Overview ◽  
Bioactive Secondary Metabolites ◽  
The Relationship ◽  
Product Chemistry

Marine sponges are well known as rich sources of biologically natural products. Growing evidence indicates that sponges harbor a wealth of microorganisms in their bodies, which are likely to be the true producers of bioactive secondary metabolites. In order to promote the study of natural product chemistry and explore the relationship between microorganisms and their sponge hosts, in this review, we give a comprehensive overview of the structures, sources, and activities of the 774 new marine natural products from sponge-derived microorganisms described over the last two decades from 1998 to 2017.

scholarly journals Novel Coumarin-Thiadiazole Hybrids and Their Cu(II) and Zn(II) Complexes as Potential Antimicrobial Agents and Acetylcholinesterase Inhibitors

2021 ◽  
Vol 22 (18) ◽  
pp. 9709
Author(s):  
Dariusz Karcz ◽  
Karolina Starzak ◽  
Ewa Ciszkowicz ◽  
Katarzyna Lecka-Szlachta ◽  
Daniel Kamiński ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Antibacterial Agents ◽  
Antimicrobial Agents ◽  
Acetylcholinesterase Inhibitors ◽  
Spectroscopic Techniques ◽  
Central Metal ◽  
Inhibition Activity ◽  
Ache Inhibition ◽  
Bacterial Strains ◽  
Gram Negative

A series of coumarin-thiadiazole hybrids and their corresponding Cu(II) and Zn(II) complexes were synthesized and characterized with the use of spectroscopic techniques. The results obtained indicate that all the coumarin-thiadiazole hybrids act as bidentate chelators of Cu(II) and Zn(II) ions. The complexes isolated differ in their ligand:metal ratio depending on the central metal. In most cases, the Zn(II) complexes are characteristic of a 1:1 ligand:metal ratio, while in the Cu(II) complexes the ligand:metal ratio is 2:1. All compounds were tested as potential antibacterial agents against Gram-positive (Staphylococcus aureus, Staphylococcus epidermidis) and Gram-negative (Escherichia coli, Pseudomonas aeruginosa) bacterial strains demonstrating activities notably lower than commercially available antibiotics. The more promising results were obtained from the assessment of antineurodegenerative potency as all compounds showed moderate acetylcholinesterase (AChE) inhibition activity

scholarly journals Secondary metabolites, letalithy and antimicrobial activity of six marine sponges from Mochima Bay, Sucre state, Venezuela.

2015 ◽  
Vol 7 (2) ◽  
pp. 225-232
Author(s):  
Raúl Cedeño Ramos ◽  
Haydelba D´Armas ◽  
María Amaro ◽  
Rosa Martínez
Keyword(s):  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Antibacterial Properties ◽  
Artemia Salina ◽  
Marine Sponges ◽  
Chemical Evaluation ◽  
Antitumor Compounds ◽  
Inhibition Zones ◽  
Aplysina Fulva ◽  
Soluble Fractions

he specimens of the sponges Aplysina lacunosa, Aplysina fulva, Cliona varians, Cinachyrella kuekenthali, Amphimedon viridis and Aaptos pernucleata, were collected in Mochima Bay, Venezuela, in October 2009 with the purpose of identifying families of secondary metabolites and evaluating the antibacterial properties, antifungal and brine shrimp lethal study of the ethyl acetate soluble fractions of these sponges. The chemical evaluation showed the presence of alkaloids, sterols, triterpenes, methylene ketone and tannins. The ethyl acetate soluble fractions of A. pernucleata, A. fulva and A. lacunosa sponges were the only ones that showed antibacterial activity against Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli; the soluble fraction of A. lacunosa showed the highest inhibition zones. The antifungal activity was not significant, except for A. pernucleata sponge wich showed mild activity against Candida albicans. The most prominent lethal activity in Artemia salina, were observed for C. kuekenthali, A. viridis and A. pernucleata sponges, with LC50 values of 4,10, 1,13 and < 0,01µg/mL respectively. These results allow profiling the sponges A. lacunosa, A. pernucleata and A. fulva as promising sources of antimicrobial compounds and the sponges C. kuekenthali, A. viridis and A. pernucleata as sources of antitumor compounds.

scholarly journals Characterization of Prodiginine Pathway in Marine Sponge-Associated Pseudoalteromonas sp. PPB1 in Hilo, Hawai‘i

2020 ◽  
Vol 4 ◽  
Author(s):  
Francis E. Sakai-Kawada ◽  
Courtney G. Ip ◽  
Kehau A. Hagiwara ◽  
Hoang-Yen X. Nguyen ◽  
Christopher-James A. V. Yakym ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Marine Sponge ◽  
Draft Genome ◽  
Marine Sponges ◽  
Consumer Products ◽  
Sponge Tissue ◽  
Tissue Samples ◽  
Biological Assays ◽  
Surface Sterilization ◽  
Host Sponge

Interest in bioactive pigments stems from their ecological role in adaptation, as well as their applications in various consumer products. The production of these bioactive pigments can be from a variety of biological sources, including simple microorganisms that may or may not be associated with a host. This study is particularly interested in the marine sponges, which have been known to harbor microorganisms that produce secondary metabolites like bioactive pigments. In this study, marine sponge tissue samples were collected from Puhi Bay off the Eastern shore of Hilo, Hawai‘i and subsequently were identified as Petrosia sp. with red pigmentation. Using surface sterilization and aseptic plating of sponge tissue samples, sponge-associated microorganisms were isolated. One isolate (PPB1) produced a colony with red pigmentation like that of Petrosia sp., suggesting an integral relationship between this particular isolate and the sponge of interest. 16S characterization and sequencing of PPB1 revealed that it belonged to the Pseudoalteromonas genus. Using various biological assays, both antimicrobial and antioxidant bioactivity was shown in Pseudoalteromonas sp. PPB1 crude extract. To further investigate the genetics of pigment production, a draft genome of PPB1 was sequenced, assembled, and annotated. This revealed a prodiginine biosynthetic pathway and the first cited-incidence of a prodiginine-producing Pseudoalteromonas species isolated from a marine sponge host. Further understanding into the bioactivity and biosynthesis of secondary metabolites like pigmented prodiginine may uncover the complex ecological interactions between host sponge and microorganism.

An Overview of Synthesis of Indole Alkaloids and Biological Activities of Secondary Metabolites Isolated from Hyrtios Species

2019 ◽  
Vol 19 (3) ◽  
pp. 194-205 ◽  
Author(s):  
M. Novanna ◽  
K.R. Ethiraj ◽  
S. Kannadasan
Keyword(s):  
Secondary Metabolites ◽  
Marine Sponge ◽  
Biological Activities ◽  
Indole Alkaloids ◽  
Marine Sponges ◽  
Review Article ◽  
Marine Organisms ◽  
Biologically Active ◽  
Synthetic Routes ◽  
Marine Natural Compounds

Marine sponges are a rich source of more than 50% of marine natural compounds that have been isolated from marine organisms. This review article is focused on the importance of biologically active and pharmaceutically important secondary metabolites extracted from one of the important classes of marine sponge Hyrtios sp. This review also deals with reported synthetic routes of some indole alkaloids extracted from the marine sponge Hyrtios sp. A range of bioactivities displayed by indole-based alkaloids is described.

scholarly journals The Use Of Marine Sponge Crude Extract To Improve The Resistance Of Tilapia Fish (Oreochromis niloticus) To Streptococcus agalactiae Infections

2019 ◽  
Vol 7 (2) ◽  
pp. 347
Author(s):  
Shifa A. Schram ◽  
Reiny A. Tumbol ◽  
Reni L. Kreckhoff
Keyword(s):  
Immune System ◽  
Survival Rate ◽  
Marine Sponge ◽  
Crude Extract ◽  
Streptococcus Agalactiae ◽  
Oreochromis Niloticus ◽  
Marine Sponges ◽  
Total Leukocyte Count ◽  
Freshwater Aquaculture ◽  
Sponge Extract

This study aims to examine the effect of the use of crude marine sponge extract on the resistance of streptococcus agalactiae infection in tilapia, Oreochromis niloticus, and to establish the effective dose of crude sponge extract in improving the immune system and the growth of the fish. The sponge used in the study was Cribrochalina sp. taken from Malalayang waters, Manado. The fish were taken from Freshwater Aquaculture Center, Tatelu. The fish were acclimatized for a week. After being acclimatized the fish were given feed added with sponge crude extract as a treatment with different concentrations of 20 g, 40 g and 60 g / Kg of feed for 14 days as much as 5% / body weight / day with the frequency of feeding twice a day at 10:00 am and at 5:00 p.m. After being treated, the fish was challenged with S. agalactiae. The data collected consisted of tilapia resistance, Total Leukocyte Count (TLC) as immune parameters and absolute growth. The results showed that the addition of crude extracts of Cribrochalina sp. into feed can increase TLC and growth of tilapia (p <0.05). The best results were achieved in fish fed with the addition of sponge crude extract of 40 g/kg feed. The survival rate of tilapia fed with treatment diet then challenged with pathogenic bacteria S. agalactiae showed the best results (100% survival rate) compared to controls (75%). In conclusion, feeding with a crude extract of Cribrochalina sp. has the potential to increase the immune system and growth of tilapia.Keywords: Crude Extract, Marine Sponges, Cribrochalina sp., Tilapia, Resistance, Streptococcus agalactiaeABSTRAKPenelitian ini bertujuan untuk mengkaji pengaruh penggunaan ekstrak kasar spons laut terhadap resistensi ikan nila dalam menghadapi serangan Streptococcus agalactiae, mengidentifikasi spons yang digunakan, serta mengukur pengaruh serta menetapkan dosis pemberian ekstrak kasar spons untuk meningkatkan sistem imun dan pengaruhnya terhadap pertumbuhan ikan nila. Spons yang digunakan dalam penelitian adalah spons Cribrochalina sp. yang diambil dari perairan Malalayang. Ikan uji diambil dari Balai Budidaya Air Tawar Tatelu, Provinsi Sulawesi Utara. Ikan diaklimatisasi selama seminggu. Setelah diaklimatisasi ikan diberi pakan yang ditambahkan dengan ekstrak kasar spons sebagai perlakuan dengan konsentrasi berbeda yaitu 0 g, 20 g, 40 g dan 60 g/kg pakan selama 14 hari sebanyak 5%/berat tubuh/hari dengan frekuensi pemberian pakan dua kali sehari yaitu jam 10.00 pagi dan jam 17.00 sore. Setelah diberi perlakuan, ikan diuji tantang dengan bakteri S. agalactiae. Data yang dikumpulkan terdiri dari kelangsunganhidup ikan nila. Total leukosit sebagai parameter imun dan pertumbuhan mutlak. Hasil penelitian mendapatkan bahwa penambahan ekstrak kasar spons Cribrochalina sp. ke dalam pakan mampu meningkatkan total leukosit dan pertumbuhan ikan nila (p<0.05). Dimana hasil terbaik dicapai pada ikan yang diberi pakan dengan penambahan ekstrak kasar spons sebanyak 40 g/kg pakan. Kelangsungan hidup ikan nila yang diberi pakan perlakuan yang diuji tantang dengan bakteri patogen menunjukkan hasil yang paling baik (tingkat kelangsungan hidup 100%) dibandingkan dengan kontrol (75%). Sebagai kesimpulan bahwa pemberian pakan dengan ekstrak kasar spons Cribrochalina sp. berpotensi untuk meningkatkan sistem kekebalan tubuh dan pertumbuhan pada ikan nila.Kata Kunci: Ekstrak kasar, Spons laut, Cribrochalina sp., Tilapia, Resistensi, Streptococcus agalactiae

scholarly journals Uji daya hambat ekstrak daun lidah mertua (Sansevieriae trifasciata folium) terhadap pertumbuhan bakteri Escherichia coli dan Streptococcus sp

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Brily Lombogia ◽  
Fona Budiarso ◽  
Widdhi Bodhi
Keyword(s):  
Escherichia Coli ◽  
Laboratory Study ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Antibacterial Effects ◽  
E Coli ◽  
Experimental Laboratory ◽  
Inhibition Zones

Abstract: Mother in law’s tongue plant has some active compounds inter alia saponin, polyphenol, and flavonoid that have antibacterial effects. This study aimed to identify whether the antibacterial effects of mother in law’s tongue leaf (Sansevieria Trifasciata) towards the growth of Escherichia coli and Streptococcus sp. This was an experimental laboratory study. The concentrations of mother in law’s tongue leaf extract were tested with well methods, as follows: 5%, 10%, 20%, and 40%. The results showed that this extract at concentration of 5%, 10%, 20%, and 40% could inhibit the growth of E. coli with the average diameters of inhibition zones as follows: 7.8 mm, 13 mm, 14.5 mm, and 17.3 mm meanwhile of Streptococcus sp. with the average diameters of inhibition zones, as follows: 4.6 mm, 9.6 mm, 13 mm, and 15.3 mm. Conclusion: Ethanol extract of mother in law’s tongue leaves (Sansevieria Trifasciata) has antibacterial activities against the growth of E. coli and Streptococcus sp. The higher the concentration is, the broader the inhibition zone is.Keywords: Sansevieriae trifasciata folium, inhibition zone, E. coli, Streptococcus sp. Abstrak: Tanaman Lidah Mertua (Sansevieria Trifasciata) memiliki senyawa aktif yaitu Saponin, Polifenol, dan Flavonoid yang mampu bekerja sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui ada tidaknya daya hambat ekstrak daun lidah mertua (Sansevieria trifasciata) terhadap pertumbuhan bakteri E. coli, dan Streptococcus sp. Jenis penelitian ini eksperimental laboratorik. Kadar ekstrak etanol daun lidah mertua (Sansevieria trifasciata) yang diujikan dengan metode sumuran yaitu 5%, 10%, 20%, dan 40%. Ekstrak etanol daun lidah mertua (Sansevieria trifasciata) dengan konsentrasi 5%, 10%, 20%, dan 40% dapat menghambat pertumbuhan bakteri E. coli dengan rerata diameter zona hambat masing-masing yaitu 7,8 mm, 13 mm, 14,5 mm, dan 17,3 mm sedangkan Streptococcus sp. dengan masing-masing rerata diameter zona hambat yaitu 4,6 mm, 9,6 mm, 13 mm, dan 15,3 mm. Simpulan: Ekstrak etanol daun lidah mertua (Sansevieria trifasciata) mempunyai aktifitas antimikroba terhadap pertumbuhan bakteri E. coli dan Streptococcus sp, dimana makin tinggi konsentrasi ekstrak daun lidah mertua, makin luas zona jernih pada media kultur bakteri E. coli dan Streptococcus sp. Kata kunci: Sansevieriae trifasciata folium, daya hambat, E. coli, Streptococcus sp.

When Apologies for Injustice Matter

2008 ◽  
Vol 13 (4) ◽  
pp. 239-247 ◽  
Author(s):  
David De Cremer ◽  
Barbara C. Schouten
Keyword(s):  
Laboratory Study ◽  
Interaction Effect ◽  
Interactive Effect ◽  
Cross Sectional ◽  
Fairness Perceptions ◽  
Organizational Survey ◽  
Experimental Laboratory

The present research examined the idea that the effectiveness of apologies on promoting fairness perceptions depends on how meaningful and sincere the apology is experienced. More precisely, it was predicted that apologies are more effective when they are communicated by an authority being respectful to others. A study using a cross-sectional organizational survey showed that an apology (relative to giving no apology) revealed higher fairness perceptions, but only so when the authority was respectful rather than disrespectful. In a subsequent experimental laboratory study the same interaction effect (as in Study 1) on fairness perceptions was found. In addition, a similar interaction effect also emerged on participants’ self-evaluations in terms of relational appreciation (i.e., feeling valued and likeable). Finally, these self-evaluations accounted (at least partly) for the interactive effect on fairness perceptions.

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