scholarly journals FORMULASI DAN UJI AKTIVITAS SABUN CAIR EKSTRAK ETANOL HERBA SELEDRI (Apium graveolens L) TERHADAP BAKTERI Staphylococcus aureus

PHARMACON ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 73
Author(s):  
Hamido Hutauruk ◽  
Paulina V. Y. Yamlean ◽  
Weny Wiyono

ABSTRACT Celery plants (Apium graveolens L.) contain flavonoids, saponins, 1% tannins, 0.033% essential oils, apiin, apigenin, choline, lipase, asparagine. which can inhibit bacterial activity. This study aims to formulate, and test the antibacterial activity of liquid celery herbal soap (Apium graveolens L) liquid soap preparations at concentrations of 1%, 2%, 4% and 8%. This study uses an experimental method by testing the parameters of the physical evaluation requirements of liquid soap preparations. Physical evaluation of the preparation includes organoleptic test, pH test, high test and foam stability, water content test, free alkali test, specific gravity test and irritation test. The results of the study on the quality test or physical evaluation of celery herbal liquid soap liquid with a concentration of 1%, 2%, 4% and 8% meet the requirements set by SNI. Antibacterial testing of celery herbal liquid soap preparations against Staphylococcus aureus bacteria observed on UV-Vis spectrophotometer produced a minimum inhibitory level of 1.267 for a concentration of 1%, 0.45 for a concentration of 2%, -0.037 for a concentration of 4%, and -0.124 for a concentration of 8 %. It can be concluded that liquid soap concentrations of 1%, 2%, 4% and 8% of celery herbal extract can be formulated as liquid soap preparations and have antibacterial activity at concentrations of 1%, 2%, 4%, and 8%. Keywords: Celery (Apium graveolens L), Liquid soap, Staphylococcus aureus, Antibacterial ABSTRAK Tanaman Seledri (Apium graveolens L.) mengandung flavonoid, saponin, tannin 1%, minyak atsiri 0,033 %, apiin, apigenin, kolin, lipase, asparagine. yang mampu menghambat aktivitas bakteri.  Penelitian ini bertujuan untuk memformulasi, serta menguji aktivitas antibakteri sediaan sabun cair ekstrak etanol herba Seledri (Apium graveolens L) pada kosentrasi 1%, 2%, 4% dan 8%. Penelitian ini menggunakan metode ekperimental dengan melakukan pengujian parameter persyaratan evaluasi fisik sediaan sabun cair. Evaluasi fisik sediaan meliputi uji organoleptik, uji pH, uji tinggi dan kestabilan busa, uji kadar air, uji alkali bebas, uji bobot jenis dan uji iritasi. Hasil penelitian pada uji kualitas atau evaluasi fisik dari sabun cair Ekstrak Etanol Herba Seledri dengan konsentrasi 1%, 2%, 4% dan 8%   memenuhi syarat yang telah ditetapkan oleh SNI. Pengujian antibakteri sediaan sabun cair Ekstrak Etanol Herba Seledri pada bakteri staphylococcus aureus yang diamati pada spektrofotometer UV-Vis menghasilkan kadar hambat minimum 1,267 untuk konsentrasi 1%, 0,45 untuk konsentrasi 2%, -0,037 untuk konsentrasi 4%, dan -0,124 untuk konsentrasi 8%. Dapat disimpulkan bahwa sabun cair kosentrasi 1%, 2%, 4% dan 8% Ekstrak Etanol Herba Seledri dapat diformulasi sebagai sediaan sabun cair yang stabil dan memiliki aktivitas antibakteri pada konsentrasi 1%, 2%, 4%, dan 8%.Kata kunci : Seledri (Apium graveolens L), Sabun cair, Staphylococcus aureus, Antibakteri

PHARMACON ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 259
Author(s):  
Riska Tuloli ◽  
Hosea Jaya Edi ◽  
Imam Jayanto

ABSTRACT Celery plants (Apium graveolens L.) and teak plants (Tectona grandis Linn.f) are plants that contain flavonoids, tannins, and saponins and has antibacterial activity against Staphylococcus aureus. This study aims to test the antibacterial effectiveness of combination of celery and teak leaf cream and evaluate cream with parameters of physical tests (organoleptic, homogeneity, pH, spreadability, adhesion) and physical stability with cycling test method. This study used an experimental laboratory method by making 5 variations concentration of extracts. Celery and teak leaf extract was extracted by maceration method. Antibacterial test used the wells method. Results of the evaluation of physical tests for organoleptic test (odor: a typical combination of extracts;color: brown;shape: semi-solid), homogeneity test (homogeneous), average of pH test is 6.02, dispersion test is 4.27 cm, adhesion test is 18.42 seconds. Based on the results of this study, concluded that the combination of celery leaf and teak leaf extract can be formulated into a cream. The cream of combination of celery and teak leafs extract are bacteriostatic and has antibacterial activity with an average diameter for formulation I 13.4 mm, formulation II 9.23 mm, formulation III 16.23 mm, formulation IV 11 mm, and formulation V 12.06 mm. Keywords : Celery (Apium graveolens L.), Teak (Tectona grandis Linn.f), Antibacterial, Cream. ABSTRAK Tanaman seledri (Apium graveolens L.) dan tanaman jati (Tectona grandis Linn.f) merupakan tanaman yang mengandung flavonoid, tanin dan saponin dan memiliki aktivitas antibakteri terhadap Staphylococcus aureus. Penelitian ini bertujuan untuk menguji aktivitas antibakteri sediaan krim kombinasi ekstrak daun seledri dan daun jati serta mengevaluasi sediaan krim dengan parameter uji sifat fisik (uji organoleptik, uji homogenitas, uji pH, uji daya sebar, uji daya lekat) dan stabilitas fisik dengan metode cycling test. Penelitian ini menggunakan metode eksperimental laboratorium dengan membuat 5 variasi konsentrasi ekstrak. Ekstrak diperoleh dengan cara maserasi. Uji antibakteri dilakukan dengan metode sumuran. Hasil evaluasi sifat fisik untuk uji organoleptik (bau: khas kombinasi ekstrak;warna:coklat;bentuk:setengah padat), uji homogenitas (susunan homogen), rata-rata uji pH 6,02, daya sebar 4,27 cm, daya lekat 18,42 detik. Berdasarkan hasil penelitian disimpulkan bahwa kombinasi ekstrak daun seledri dan daun jati dapat diformulasikan menjadi sediaan krim. Sediaan krim kombinasi ekstrak daun seledri dan daun jati bersifat bakteriostatik dan memiliki aktivitas antibakteri dengan diameter rata-rata untuk formula I 13,4 mm, formula II 9,23 mm, formula III 16,23 mm, formula IV 11 mm, dan formula V 12,06 mm. Kata Kunci : Seledri (Apium graveolens L.), Jati (Tectona grandis Linn.f), Antibakteri, Krim


2020 ◽  
pp. 1-10
Author(s):  
Yuni Anggraeni ◽  
Fakhrun Nisa' ◽  
Ofa Suzanti Betha

Patchouli oil obtained from patchouli plants (Pogostemon cablin Benth.) has been widely used as an antibacterial agent in liquid and solid soap preparation. In this research, patchouli oil was added to liquid soap formula which use sodium lauryl ether sulphate (SLES) surfactant. The purpose of this study was to determine the physical characteristics and antibacterial activity of patchouli oil liquid soaps based on SLES surfactant. The Liquid soaps were prepared into four formulas with variation of patchouli oil concentration that were F0 (0.00% w/w), F1 (0.05% w/w), F2 (0.50% w/w), and F3 (1.00% w/w). The liquid soaps were evaluated including organoleptic, microscopic property, physical stability by using centrifuge test, pH, foam height and stability, viscosity and rheology, and antibacterial activity against Staphylococcus aureus ATCC 25923 by using disc diffusion method and chloramphenicol as a positive control. The results showed that all liquid soap formulas had following characteristics: translucent except F3 slightly murky, homogen, stable after centrifuge test, pH between 6.55 – 6.66 that were no significantly different and met SNI standards of liquid soap, foam height between 2.67 – 3.23 cm that were no significantly different and stable after 5 minutes of testing, viscosity between 2237 – 2397 cPs relatively equal in all formulas with pseudoplastic rheological properties. The patchouli oil liquid soaps had antibacterial activity against Staphylococcus aureus ATCC 25923, where F0, F1, F2, F3 and positive control result in inhibition zone diameter of 11.26±2.60 mm, 14.60 ± 2.45mm, 15.51 ± 0.44 mm, 17.97 ± 0.71 mm and 19.00 ± 3.92 mm respectively. Based on the ANOVA one way test, the addition of 1% patchouli oil gave a significant effect on the inhibition zone diameter compared to F0 (without patchouli oil)


3 Biotech ◽  
2021 ◽  
Vol 11 (4) ◽  
Author(s):  
Eswaran Tamilselvi ◽  
Arjunan Karuppaiah ◽  
Govindarajan Shyamala ◽  
Subramanian Shobana ◽  
Parimelazhagan Thangaraj ◽  
...  

PHARMACON ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 226
Author(s):  
Garry Clements ◽  
Paulina V. Y. Yamlean ◽  
Widya Astuty Lolo

ABSTRACTCelery herbs (Apium graveolens L.) have content flavonoid, saponin and tannin. Flavonoids have anti-inflammatory properties, antioxidants and antibacterials. This study aims to determine whether the ethanol extract of celery herbs can be formulated into cream preparations, test the quality of preparations at concentrations of 5%, 10% and 15%. These methods of research are experimental laboratories by making the supply of self-sustaining herbal celery ethanol extract cream and testing the quality of the cream preparation,  as well as testing of antibacterial activity was carried out using the liquid dilution method and measuring value of  Minimum Inhibitory Concentration  and Minimum Bactericidal Concentration using a UV-Vis Spectrophotometer. This research shows that celery herbs ethanol extract can be formulated into cream preparations, fulfilling quality test of organoleptic, scatter power, adhesion and cycling test, but that doesn’t meet the requirements for homogeneity tests and pH tests and has antibacterial activity which not only inhibits but kills bacteria in preparations of 5% and 15%Keywords: Cream Celery herb extract, Antibacterial, Staphylococcus aureus, UV-Vis spectrophotometer ABSTRAK Herba Seledri (Apium graveolens L.) memiliki kandungan flavonoid, saponin dan tannin. Flavonoid memiliki khasiat sebagai antiinflamasi, antioksidan dan antibakteri. Penelitian ini bertujuan untuk mengetahui apakah ekstrak etanol herba Seledri dapat diformulasikan menjadi sediaan krim, menguji mutu sediaan pada konsentrasi 5%, 10% dan 15%. Metode penelitian ini experimental laboratorium dengan membuat Sediaan Krim Ekstrak Etanol Herba Seledri dan menguji mutu sediaan krim, serta   menguji aktivitas antibakteri menggunakan metode dilusi cair dan mengukur nilai KBM dan KHM menggunakan Spektrofotometer UV-Vis. Hasil penelitian menujukkan bahwa ekstrak etanol herba Seledri dapat diformulasikan menjadi sediaan krim, memenuhi uji mutu sediaan organoleptik, daya, sebar, daya hambat dan stabilitas  namun tidak memenuhi persyaratan untuk uji homogenitas dan uji pH serta mempunyai aktivitas antibakteri yang membunuh bakteri pada sediaan berkonsentrasi 5% dan 15%.Kata Kunci : Krim Ekstrak herba Seledri, Antibakteri, Staphylococcus aureus, Spektrofotometer UV-Vis


PHARMACON ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 801
Author(s):  
Yulita Ni Nyoman Tri Sukartiningsih ◽  
Hosea Jaya Edi ◽  
Jainer P. Siampa

ABSTRACT Kaliandra Leaf (Calliandra surinamensis Benth) contains flavonoid compounds, saponins and tannins which are able to inhibit the antibacterial activity. This study aims to test the antibacterial effectiveness of gel preparation of ethanol extracts of kaliandra leaf to obtain the greatest formula, and prove the Kaliandra leaf gel preparation has good physical stability. This study uses a laboratory experimental method. Gel formula is made as many as 5 formulas with variations in the concentration of 2%, 3%, 4%, 5%, and 6% of w : v. Kaliandra leaf extraction was carried out by maceration method using 96% ethanol. The antibacterial test of the ethanol extracts of Kaliandra leaf gel using the method of welling against Staphylococcus aureus bacteria produced a moderate inhibition of 7.0 ± 0.63 mm at a concentration of 6%. The physical evaluation of the preparation includes organoleptic test, homogeneity test, syneresis test, scatter power test, adhesion test, pH test, cycling test, and sterility test. All tests are carried out before and after the cycling test. The results of the study on the preparation fulfilled the physical evaluation requirements before and after the cycling test. It can be concluded that the concentration of 6% of ethanol extracts of Kaliandra leaves could be formulated as a gel preparation that is physically stable and has moderate antibacterial activity. Keywords: Kaliandra (Calliandra surinamensis Benth), Gel preparations, Staphylococcus aureus antibacterial.  ABSTRAK Daun Kaliandra (Calliandra surinamensus Benth) mengandung senyawa flavonoid, saponin dan tannin yang mampu menghambat aktivitas antibakteri. Penelitian ini bertujuan untuk menguji efektivitas antibakteri sediaan gel ekstrak etanol daun Kaliandra untuk mendapatkan formula terbesar, serta membuktikan sediaan gel daun Kaliandra mempunyai stabilitas fisik yang baik. Penelitian ini menggunakan metode ekperimental laboratorium. Formula sediaan gel dibuat sebanyak 5 formula dengan variasi konsentrasi 2%, 3%, 4%, 5%, dan 6% b/v. Ekstraksi daun Kaliandra dilakukan dengan metode maserasi menggunakan etanol 96%.  Penelitian uji antibakteri sediaan gel ekstrak etanol daun Kaliandra menggunakan metode sumuran pada bakteri staphylococcus aureus menghasilkan daya hambat yang sedang yaitu 7,0±0,63 mm pada konsentrasi 6%. Evaluasi fisik sediaan meliputi uji organoleptik, uji homogenitas, uji sineresis, uji daya sebar, uji daya lekat, uji pH, uji cycling test, dan uji sterilitas. Semua pengujian dilakukan sebelum dan sesudah cycling test. Hasil penelitian sediaan memenuhi persyaratan eveluasi fisik sebelum uji cycling test dan sesudah cycling test. Dapat disimpulkan pada kosentrasi 6% ekstrak etanol daun Kaliandra dapat diformulasi sebagai sediaan gel yang stabil secara fisik dan memiliki aktivitas antibakteri yang sedang. Kata kunci : Kaliandra (Calliandra surinamensis Benth), Sediaan Gel, Antibakteri Staphlococcous  aureus.


2021 ◽  
Vol 4 (1) ◽  
pp. 43-50
Author(s):  
Asiska Permata Dewi ◽  
Dini Mardhiyani

Ketapang (Terminalia catappa L.) is traditionally used by the community to treat infections of the skin caused by bacteria or fungi. In this study, T. catappa leaves extract was added to the liquid soap formula as an antibacterial. The purpose of this study was to determine the secondary metabolite compounds contained in T. catappa leaves extract, physical evaluation of the preparation, and antibacterial activity of liquid soap. Liquid soap formula is made with various concentrations of T. catappa leaves extract F0 (0%), F1 (1%), F2 (2%), and F3 (3%). The resulting soap was evaluated for organoleptic, pH, high foam, homogeneity, irritation, and its activity against Staphylococcus aureus, Staphylococcus epidermidis, and Escherichia coli using the disc diffusion method. The results showed that the T. catappa leaves extract contained flavonoids, tannins, saponins, and triterpenoids. The liquid soap formula F0 is clear, while F1, F2, and F3 have the characteristics of brown-dark brown, homogeneous, pH between 4.6-5.2, foam stability between 67-72%, which is not significantly different and stable after five minutes of testing, and it does not irritate the skin. Terminalia catappa leaves extracts liquid soap has antibacterial activity at a concentration of 1%, 2%, and 3%, with the largest inhibition zone diameter produced by S. aureus.


2021 ◽  
Vol 3 (1) ◽  
pp. 45-57
Author(s):  
Rinaldi Rinaldi ◽  
Fauziah Fauziah ◽  
Rizka Mastura

Citronella (Cymbopogon Nardus.L) contains essential oil compounds, saponins, polyphenols and flavonoids which are antibacterial. The use of active compounds of Citronella is formulated into antibacterial liquid soap preparations. This study aims to determine the characteristics of liquid soap and its inhibition against the growth of Staphylococcus aureus. This research is experimental to formulate liquid soap containing ethanol extract of lemongrass sticks at concentrations of 9% (F1), 18% (F2) and 24% (F3). The test parameters for liquid soap preparations include organoleptic test (shape, aroma, color), homogeneity test, pH test, foam height test, foam stability test, free alkaline test and inhibition test against the growth of Staphylococcus aureus using the Kirby-Bauer method. The results showed that the liquid soap preparations in the F1, F2 and F3 formulas were liquid, had a distinctive aroma of Citronella and were brown in color, homogeneous. The pH values, foam height, foam stability and free alkaline content of liquid soap were F1 (12; 70 mm; 85.7% and 0.14%), F2 (11. 80 mm, 87.5% and respectively). 0.10%), F3 (10.100 mm, 90% and 0.08%). The formula inhibition zone diameters are 32.06 mm, 25.6 mm and 29.2 mm, respectively. The conclution is the characteristics of the liquid soap with the ethanol extract of Citronella (Cymbopogon Nardus.L) meet the requirements as liquid soap. The greatest inhibitory effectiveness on the growth of Staphylococcus aureus was a formula at a concentration of 24% (F3) with a strong category. 


EKOLOGIA ◽  
2019 ◽  
Vol 19 (2) ◽  
pp. 80-88
Author(s):  
Atika Pratiwi ◽  
Ella Noorlaela ◽  
Siti Mahyuni

Bacteria that cause acne include Staphylococcus aureus and Propionibacterium acnes. Nutmeg leaves are plants that contain antibacterial substances that can inhibit bacterial growth. The purpose of this study is to make soap preparations from nutmeg leaf extracts and find out the formula that is most effective in inhibiting the growth of P.acnes and Staphylococcus aureus bacteria. Nutmeg leaves were extracted using maceration method with 96% ethanol solvent. Liquid soap preparations are made in 4 formulas. Antibacterial activity test was carried out by the dilution method for MIC and disk diffusion methods to determine LDH liquid soap preparations. Based on the test of the antibacterial activity of 96% ethanol extract, nutmeg leaves have the ability to inhibit the growth of Staphylococcus aureus with a MIC value at a concentration of 0.5% and negative on Propionibacterium acnes. Liquid soap preparations in F1 (extract 2%) have LDH values of 9.87 ± 0.41 mm; F2 (extract 4%) has an LDH value of 10.50 ± 0.35 mm; F3 (extract 8%) has an LDH value of 10.70 ± 0.25 mm; F4 (extract 10%) has an LDH value of 11.87 ± 0.25 mm. The highest LDH value is F4 with a concentration of 10% extract, LDH F4 is a formula that has the most antibacterial activity approaching LDH positive control JF Sulfur which has an LDH value of 13.25 ± 0.25 mm


2013 ◽  
Vol 821-822 ◽  
pp. 301-304
Author(s):  
Jue Chen He ◽  
Jun Li

The study is to weave the yarns of silver-plated fiber and photocatalyst fiber together to make 18 kinds of fabrics with different woven proportions and structures, and conduct antibacterial experiments on the fabrics with agar diffusion plate method. The experimental results show that for the strains of E.coli and Staphylococcus aureus, with the increasing woven proportion of silver-plated fiber and photocatalyst fiber, the width of inhibition zone grows; and in the condition of the same woven proportion of the two fibers, the plain weave fabric has the best antibacterial activity. And overall considering the antibacterial activity, cost and safety of the fabric, the plain weave fabric with 12.5% distribution ratio of silver-plated fiber is the optimal antibacterial fabric among the 18 samples.


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