scholarly journals UJI DAYA HAMBAT SEDIAAN SABUN CAIR EKSTRAK DAUN PALA (Myristica fragrans houtt) TERHADAP Propionibacterium acnes dan Staphylococcus aureus

EKOLOGIA ◽  
2019 ◽  
Vol 19 (2) ◽  
pp. 80-88
Author(s):  
Atika Pratiwi ◽  
Ella Noorlaela ◽  
Siti Mahyuni
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Propionibacterium Acnes ◽  
Ethanol Extract ◽  
Positive Control ◽  
Dilution Method ◽  
Liquid Soap ◽  
Leaf Extracts ◽  
Myristica Fragrans Houtt ◽  
Ldh Value

Bacteria that cause acne include Staphylococcus aureus and Propionibacterium acnes. Nutmeg leaves are plants that contain antibacterial substances that can inhibit bacterial growth. The purpose of this study is to make soap preparations from nutmeg leaf extracts and find out the formula that is most effective in inhibiting the growth of P.acnes and Staphylococcus aureus bacteria. Nutmeg leaves were extracted using maceration method with 96% ethanol solvent. Liquid soap preparations are made in 4 formulas. Antibacterial activity test was carried out by the dilution method for MIC and disk diffusion methods to determine LDH liquid soap preparations. Based on the test of the antibacterial activity of 96% ethanol extract, nutmeg leaves have the ability to inhibit the growth of Staphylococcus aureus with a MIC value at a concentration of 0.5% and negative on Propionibacterium acnes. Liquid soap preparations in F1 (extract 2%) have LDH values of 9.87 ± 0.41 mm; F2 (extract 4%) has an LDH value of 10.50 ± 0.35 mm; F3 (extract 8%) has an LDH value of 10.70 ± 0.25 mm; F4 (extract 10%) has an LDH value of 11.87 ± 0.25 mm. The highest LDH value is F4 with a concentration of 10% extract, LDH F4 is a formula that has the most antibacterial activity approaching LDH positive control JF Sulfur which has an LDH value of 13.25 ± 0.25 mm

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BUAH MAHKOTA DEWA (Phaleria macrocarpa) TERHADAP STAPHYLOCOCCUS AUREUS

2019 ◽  
Vol 9 (02) ◽  
pp. 134-139
Author(s):  
Yunilda Rosa ◽  
Masnir Alwi ◽  
Monica Julianti
Keyword(s):  
Staphylococcus Aureus ◽  
Experimental Study ◽  
Antibacterial Activity ◽  
Inhibitory Concentration ◽  
Skin Diseases ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Dilution Method ◽  
Phaleria Macrocarpa

Phaleria macrocarpa is one of the medicinal plants that many people use as a traditional medicinal which has many benefits, one of which is as a medicine for skin diseases. The content of flavonoid compounds in Phaleria macrocarpa believed to have potential as an antibacterial. The purpose of this study was to know the antibacterial activity of the ethanol extract of the Phaleria macrocarpa to inhibized Staphylococcus aureus bacteria. This research is an experimental study carried out by the liquid dilution method (KHM)and disk diffusion (KBM) of the extract of the Phaleria macrocarpa is divided into 6 concentrations namely 5%, 10% and 15% (KHM) and 40%, 50% and 60% (KBM). The positive control used is clindamycin and the negative control is aquadest. Data analysis uses univariate. Based on observations of the minimum inhibitory concentration of the extract of the fruit of the Phaleria macrocarpa which is at of 15%, while the minimum killed concentration is formed at of 40% with an average of 6.36 mm, and an average of zones formed on the positive control of 9.53 mm. From the results of the study, it was concluded that the extract of the fruit of the Phaleria macrocarpa can inhibit and kill the growth of Staphylococcus aureus bacteria.

UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN MEKAI (Pycnarrhena cauliflora DIELS.) TERHADAP Staphylococcus aureus

2020 ◽  
Vol 6 (2) ◽  
pp. 246
Author(s):  
Yayuk Bulam Sarifati ◽  
Sjarif Ismail ◽  
Khemasili Kosala
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antimicrobial Agents ◽  
Statistical Tests ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Control Measurement ◽  
Eye Pain

Mekai leaves (Pycnarrhena cauliflora Diels.) (P. cauliflora). Are known to contain flavonoid compounds, tannins and phenolics that act as antibacterial agents and are used in the treatment of eye pain. Staphylococcus aureus (S. aureus) is one of the examples of bacterial diseases of eye pain and also a major cause of many infections in communities and health facilities with cases of resistance to various antimicrobial agents. The purpose of this study was to prove the antibacterial activity of mekai leaves ethanol extract against S. aureus bacteria. This research is an experimental research. The stages of this research began by extracting mekai leaves using maceration method with 96% ethanol solvent. Antibacterial activity was tested by the disc method (Kirby-Bauer) using ethanol extract concentrations of mekai leaves (EPC) 20%, 30%, 40%, 50%, 60%, 70%, and 80%, positive control using 25 μg amoxicillin and negative control using DMSO 10%. The measurement results of inhibition zones of mekai leaf ethanol extract 20%, 30%, 40%, 50%, 60%, 70% and 80% respectively were 8.32 mm, 8.32 mm, 8.32 mm, 8.67 mm, 9.00 mm, 8.67 mm, and 8.33 mm. While the positive control measurement of 25 μg amoxicillin against S. aureus is 28.67 mm and the measurement of 10% negative DMSO control does not produce inhibitory zones, so it can be concluded that the ethanol extract of mekai leaves has antibacterial activity, but the area of ​​inhibition zone produced is smaller than amoxicillin 25 µg. The results of statistical tests using Mann Whitney between negative controls with all EPC concentrations obtained significant differences with p values ​​<0.05, it can be concluded that there is antibacterial activity produced at all EPC concentrations.

scholarly journals Antibacterial activities of combination of ethanol extract of Delima fruit (Punica granatum L.) and Sereh stem (Cymbopogon citratus) on Staphylococcus aureus

2019 ◽  
Vol 6 (12) ◽  
pp. 5037
Author(s):  
Melia Pebrina ◽  
Indah Komala Sari ◽  
Eliza Arman ◽  
Honesty Diana Morika
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Punica Granatum ◽  
Antibacterial Activities ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Randomized Design ◽  
Pomegranate Peel Extract

ABSTRACT Background: Pomegranates contain chemical saponins and flavonoids while lemongrass stems have flavonoid chemical compounds. Substances that can inhibit the linking of bacteria are saponins and flavonoids. The most common microorganism found in ulcus diabetikum (ulcer diabetes) is Staphylococcus aureus. The purpose of this study was to determine the antibacterial activity of a combination of pomegranate ethanol extract and ethanol extract of lemongrass stems against S. aureus bacteria.Methods: This study used an experimental design with a complete randomized design study divided into 5 groups: groups 1 (75:25), 2 (50:50), 3 (25:75), positive control (tetracycline) and negative control. Manufacture of pomegranate peel extract and lemongrass stems was done by maceration method for further rotary, after the extract was obtained tested for antibacterial activity by diffusion method using a cylinder. With the test used analysis of variance one way.Results: The results of this study showed that the combination of pomegranate ethanol extract and citronella stem ethanol extract showed activity against S. aureus with a ratio of 15 ul:5 ul, 15 ul:15 ul, 5 ul:15 ul with inhibitory diameter of  respectively 13 mm, 11 mm, 8 mm. Whereas for positive control with chloramphenicol, the inhibition area is 9 mm.Conclusions: Statistically the combination of pomegranate ethanol extract and lemongrass stems has antibacterial power which uses p=0.005 and p<0.05. The combination of pomegranate ethanol extract and lemongrass stems has very strong antibacterial activity against S. aureus due to inhibition zones of 10-20 mm.

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK DAN FRAKSI KARANG LUNAK Sinularia SP. DI TELUK MANADO

PHARMACON ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 114
Author(s):  
Lyone Katiandagho ◽  
Defny S Wewengkang ◽  
Sri Sudewi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Soft Coral ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Chloroform Fraction ◽  
Water Fraction

ABSTRACT Soft Coral Sinularia sp.has been shown to have antibacterial activity. This research aims to determine the antibacterial activity of marine  organisms obtained from Manado bay. The method in this study is the diffusion method with positive control chloramphenicol and negative control of methanol in Escerichia coli and Sthaphylococcus aureus. The result showed that extracts, chloroform fraction and methanol-water fraction were obtained inhibiting category of Sthapylococcus aureus bacteria, but in the chloroform fraction and ethanol extract can inhibit Escerichia coli bacteria categorized as strong. With it can be conlueded that the extract and fraction of soft Sinularia sp. have bioactive compounds with a broth spectrum of antibacterial activity.Keywords: Sinularia sp., Chloramphenicol, Escerichia coli, Sthaphylococcus aureus, and antibacterial. ABSTRAK Karang lunak Sinularia sp. telah terbukti memiliki aktivitas antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri pada organisme biota laut yang diperoleh dari teluk Manado. Metode dalam penelitian ini adalah metode difusi agar dengan kontrol positif kloramfenikol dan kontrol negatit metanol pada Escherichia coli dan Staphylococcus aureus. Hasil penelitian menunjukkan bahwa ekstrak, fraksi kloroform, dan fraksi metanol-air dapat menghambat bakteri Staphylococcus aereus dikategorikan sedang, namun pada fraksi kloroform dan ekstrak etanol dapat menghambat bakteri Escerichia coli dikategorikan kuat. Dengan demikian dapat disimpulkan bahwa ekstrak dan fraksi karang lunak Sinularia sp.memiliki senyawa bioaktif dengan spektrum yang luas terhadap aktivitas antibakteri.Kata kunci: Sinularia sp, Kloramfenikol, Escherichia coli, Staphylococcus aureus, dan antibakteri

Karakteristik Fisik dan Aktivitas Antibakteri Sabun Cair Minyak Nilam (Pogostemon cablin Benth.) yang Berbasis Surfaktan Sodium Lauril Eter Sulfat

2020 ◽  
pp. 1-10
Author(s):  
Yuni Anggraeni ◽  
Fakhrun Nisa' ◽  
Ofa Suzanti Betha
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Foam Height ◽  
Inhibition Zone ◽  
Positive Control ◽  
Centrifuge Test ◽  
Liquid Soap ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Pogostemon Cablin

Patchouli oil obtained from patchouli plants (Pogostemon cablin Benth.) has been widely used as an antibacterial agent in liquid and solid soap preparation. In this research, patchouli oil was added to liquid soap formula which use sodium lauryl ether sulphate (SLES) surfactant. The purpose of this study was to determine the physical characteristics and antibacterial activity of patchouli oil liquid soaps based on SLES surfactant. The Liquid soaps were prepared into four formulas with variation of patchouli oil concentration that were F0 (0.00% w/w), F1 (0.05% w/w), F2 (0.50% w/w), and F3 (1.00% w/w). The liquid soaps were evaluated including organoleptic, microscopic property, physical stability by using centrifuge test, pH, foam height and stability, viscosity and rheology, and antibacterial activity against Staphylococcus aureus ATCC 25923 by using disc diffusion method and chloramphenicol as a positive control. The results showed that all liquid soap formulas had following characteristics: translucent except F3 slightly murky, homogen, stable after centrifuge test, pH between 6.55 – 6.66 that were no significantly different and met SNI standards of liquid soap, foam height between 2.67 – 3.23 cm that were no significantly different and stable after 5 minutes of testing, viscosity between 2237 – 2397 cPs relatively equal in all formulas with pseudoplastic rheological properties. The patchouli oil liquid soaps had antibacterial activity against Staphylococcus aureus ATCC 25923, where F0, F1, F2, F3 and positive control result in inhibition zone diameter of 11.26±2.60 mm, 14.60 ± 2.45mm, 15.51 ± 0.44 mm, 17.97 ± 0.71 mm and 19.00 ± 3.92 mm respectively. Based on the ANOVA one way test, the addition of 1% patchouli oil gave a significant effect on the inhibition zone diameter compared to F0 (without patchouli oil)

scholarly journals FORMULASI DAN UJI AKTIVITAS ANTIBAKTERI KRIM EKSTRAK ETANOL HERBA SELEDRI (Apium graveolens L.) TERHADAP BAKTERI Staphylococcus aureus

PHARMACON ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 226
Author(s):  
Garry Clements ◽  
Paulina V. Y. Yamlean ◽  
Widya Astuty Lolo
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Ethanol Extract ◽  
Apium Graveolens ◽  
Dilution Method ◽  
Herb Extract

ABSTRACTCelery herbs (Apium graveolens L.) have content flavonoid, saponin and tannin. Flavonoids have anti-inflammatory properties, antioxidants and antibacterials. This study aims to determine whether the ethanol extract of celery herbs can be formulated into cream preparations, test the quality of preparations at concentrations of 5%, 10% and 15%. These methods of research are experimental laboratories by making the supply of self-sustaining herbal celery ethanol extract cream and testing the quality of the cream preparation,  as well as testing of antibacterial activity was carried out using the liquid dilution method and measuring value of  Minimum Inhibitory Concentration  and Minimum Bactericidal Concentration using a UV-Vis Spectrophotometer. This research shows that celery herbs ethanol extract can be formulated into cream preparations, fulfilling quality test of organoleptic, scatter power, adhesion and cycling test, but that doesn’t meet the requirements for homogeneity tests and pH tests and has antibacterial activity which not only inhibits but kills bacteria in preparations of 5% and 15%Keywords: Cream Celery herb extract, Antibacterial, Staphylococcus aureus, UV-Vis spectrophotometer ABSTRAK Herba Seledri (Apium graveolens L.) memiliki kandungan flavonoid, saponin dan tannin. Flavonoid memiliki khasiat sebagai antiinflamasi, antioksidan dan antibakteri. Penelitian ini bertujuan untuk mengetahui apakah ekstrak etanol herba Seledri dapat diformulasikan menjadi sediaan krim, menguji mutu sediaan pada konsentrasi 5%, 10% dan 15%. Metode penelitian ini experimental laboratorium dengan membuat Sediaan Krim Ekstrak Etanol Herba Seledri dan menguji mutu sediaan krim, serta   menguji aktivitas antibakteri menggunakan metode dilusi cair dan mengukur nilai KBM dan KHM menggunakan Spektrofotometer UV-Vis. Hasil penelitian menujukkan bahwa ekstrak etanol herba Seledri dapat diformulasikan menjadi sediaan krim, memenuhi uji mutu sediaan organoleptik, daya, sebar, daya hambat dan stabilitas  namun tidak memenuhi persyaratan untuk uji homogenitas dan uji pH serta mempunyai aktivitas antibakteri yang membunuh bakteri pada sediaan berkonsentrasi 5% dan 15%.Kata Kunci : Krim Ekstrak herba Seledri, Antibakteri, Staphylococcus aureus, Spektrofotometer UV-Vis

scholarly journals Antibacterial Activity of Cold Powder Preparation of (Ethanol Extract) Starfruit Leaf (Averrhoa bilimbi Linn.) Against Propionibacterium acnes

2020 ◽  
Vol 6 (2) ◽  
pp. 120-128
Author(s):  
Helen Anjelina Simanjuntak ◽  
Kasta Gurning ◽  
Verawati Br. Sinaga
Keyword(s):  
Antibacterial Activity ◽  
Medicinal Plant ◽  
Research Method ◽  
Propionibacterium Acnes ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Distilled Water ◽  
Powder Preparation

Acne is a skin disease that occurs due to chronic trade that is triggered by the bacterium Propionibacterium acnes. Starfruit leaf (Averrhoa bilimbi Linn.) as a traditional medicinal plant which has antibacterial activity. The purpose of this study was to determine the antibacterial activity from ethanol extract in the preparations cold powder of starfruit leaf (Averrhoa bilimbi Linn.) againts Propionibacterium acnes. The research method was carried out experimentally, and testing for antibacterial activity was carried out using agar diffusion methods. Cold powder formulation such as F1 (5%), F2 (10%), F3 (15%), F4 (20%), positive control with clindamycin, negative control with distilled water. The results showed that the F3 (15%) and F4 (20%) formulations were more effective in inhibiting the growth of Propionibacterium acnes bacteria with inhibition zone diameters of 13.66 mm and 15.16 mm in the strong category

scholarly journals Test Activities Antibakteriekstrak Ethanol Young And Old Leaves Of The Soursop(Annonamuricata L.) Against Staphylococcus aureus

2018 ◽  
Vol 1 (2) ◽  
pp. 135-143 ◽  
Author(s):  
Dwi Latifah Sari ◽  
Sudarmi Sudarmi ◽  
Popi Patilaya
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Skin Diseases ◽  
Ethanol Extract ◽  
Positive Control ◽  
Annona Muricata ◽  
Phytochemical Screening ◽  
Activity Test ◽  
Young Leaves

Soursop (Annona muricata L.) has been used for generations by some communities in Indonesia to treat diseases. Soursop leaves are used as an alternative treatment for cancer, by boiling them in the water and consuming it. In addition to the treatment of cancer, soursop plant is also used for the treatment of skin diseases such as boils. This study aimed to determine the antibacterial activity of ethanol extract of soursop leaves of young and old against Staphylococcus aureus, determine the content of secondary metabolites, and find out the diameter of inhibitory young leaves and old leaves of soursop in inhibiting Staphylococcus aureus. Soursop leaves were extracted by maceration using ethanol 96%. The extract obtained was then carried out to phytochemical screening. Antibacterial activity test was conducted using disc the Kirby-Bauerdiffusion. This study used 6 concentrations of 500 mg/mL, 400 mg/mL, 300 mg/mL, 200 mg/mL, 100 mg/mL, 80 mg/mL, 60 mg/mL, 40 mg/mL, 20 mg/mL, 10 mg/mL and 1 mg/mL. A positive control used soursop leaf extract of young and old while a negative one used DMSO 10%. Based on phytochemical screening, ethanol extract of soursop leaves contained alkaloids, flavonoids, saponins, tannins, steroids/triterpenoids and glycosides. The antibacterial activity of ethanol extract of leaves of young soursop was higher in inhibitory diameter than the ethanol extract of old soursop leaves. It could be seen that a concentration of 500 mg/mL was equal to 10.87 mm and 8.68 mm; a concentration of 400 mg/mL was equal to 9.15 mm and 7.3 mm; a concentration of 300 mg/mL was equal to 8.34 mm and 6.30 mm; a concentration of 200 mg/mL was equal to 8.23 mm and 7.08 mm; a concentration of 100 mg/mL was equal to 6.32 mm and 6.18 mm, and a concentration of 80 mg/mL did not leave a diameter inhibitory to bacteria Staphylococcus aureus. Young soursop leaf ethanol extract was more effective than the old soursop one against Staphylococcus aureus.

scholarly journals Antibacterial Activities of Kacapiring Leaf Ethanol Extract (Gardenia jasminoides Ellis) on the Bacteria of Staphylococcus aureus and Propionibacterium acnes

MEDULA ◽  
2019 ◽  
Vol 6 (3) ◽  
Author(s):  
Nuralifah Nuralifah ◽  
Fery Indradewi Armadani ◽  
Ni Nyoman Fitri Astari
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Propionibacterium Acnes ◽  
Ethanol Extract ◽  
The Body ◽  
Plate Technique ◽  
Gardenia Jasminoides ◽  
Gardenia Jasminoides Ellis ◽  
Inhibitory Power

ABSTRACTBackground and Objective(s): Infection is the process of entering pathogenic bacteria or microorganisms into the body capable of causing disease. Bacterial infections can cause skin infections such as acne. Acne is inflammation that is accompanied by blockage of the oil glands of the skin and hair (pilosebasea duct). Gardenia jasminoides Ellis is a plant that empirically can be used to treat infectious diseases caused by bacteria. This study was conducted to determine the antibacterial activity of the extracts of kacapiring leaves (Gardenia jasminoides Ellis) against Staphylococcus aureus and Propionibacterium acnes bacteria. Methods: Making extracts was done by maceration method  using  96% ethanol. The concentrations of extracts  used  were 5%, 10% and  15%,  with chloramphenicol and clindamycin as positive controls and distilled water as negative controls. Antibacterial activity was tested by the Cup-plate technique. Results: The antibacterial activity of kacapiring leaves ethanol extract to S. aureus showed that at concentrations of 5%, 10% and 15% the inhibitory power diameters values were respectively 5.58 mm, 9.3 mm and 11.41 mm while the antibacterial activity of extracts kacapiring leaves ethanol to P. acnes bacteria at concentrations of 5%, 10% and 15% had inhibitory power diameters of 6.33 mm, 9.25 mm and 11 mm respectively. Conclusion: Ethanol extract of kacapiring leaves has antibacterial activity against Staphylococcus aureus and Propionibacterium acnes bacteria. At a concentration of 5% and 10%, it shows the moderate category and at a concentration of 15% shows a strong category.Keywords: antibacterial, cup-plate technique , gardenia jasminoides

UJI AKTIVITAS ANTIBAKTERI DARI EKSTRAK ETANOL DAUN PERIA LAUT (Colubrina Asiatica L.) TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS DAN ESCHERICHIA COLI

2019 ◽  
Vol 2 (1) ◽  
pp. 14-18
Author(s):  
Firdaus Fahdi ◽  
Harwitavia Harwitavia ◽  
Herviani Sari
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Antibiotic Drugs ◽  
Escherichia Coli Bacteria

The discovery of new antibiotic drugs is getting more and more reactive. The plant of the peria laut leaf is one of the drugs that is often used as a traditional medicine and contains bioactive compounds of polyphenols, flavonoids, and saponins, which can inhibit antibacterial growth. Purpose of this study was to determine the antibacterial activity of ethanol extract of peria laut leaves (Colubrina asiatica L.) against Staphylococcus aureus and Escherichia coli bacteria. Method the experimental, of the sample used was concentrated marine peria laut leaf extract of 25mg/ml, 50mg/ml, 75mg/ml, 100mg/ml, 200mg/ml, 300mg/ml, 400mg/ml, and 500mg/ml, positive control of amoxicillin tablet 500 mg, negative control of dimethylsulfoxide with the method of disc diffusion testing using media Nutrient Agar. Results the showed thet the peria laut leaf extract positively contained bioactive alkaloid compounds, flavonoids, saponins, steroids, and tannins, and had inhibitory effects on Staphylococcus aureus and Escgerichia coli bacteria with various concentrations which had been tested on the average area of the highest inhibition zone of 18,6 mm in Staphylococcus aureus bacteria, and 10,2 mm in Escherichia coli bacteria. Conclusion peria laut leaf extract (Colubrina asiatica L.) has antibacterial activity against Staphylococcus aureus and Escherichia coli bacteria in the most inhibitory zone at a concentration of 500mg/ml with a diameter of 18,6 in Staphylococcus aureus bacteria and 10,2 in Escherichia coli.

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