scholarly journals FORMULASI SEDIAAN KRIM KOMBINASI EKSTRAK DAUN SELEDRI (Apium graveolens L.) DAN DAUN JATI (Tectona grandis Linn.F) SEBAGAI ANTIBAKTERI TERHADAP Staphylococcus aureus

PHARMACON ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 259
Author(s):  
Riska Tuloli ◽  
Hosea Jaya Edi ◽  
Imam Jayanto
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Tectona Grandis ◽  
Test Method ◽  
Apium Graveolens ◽  
Homogeneity Test ◽  
Cycling Test ◽  
Physical Tests ◽  
Dispersion Test

ABSTRACT Celery plants (Apium graveolens L.) and teak plants (Tectona grandis Linn.f) are plants that contain flavonoids, tannins, and saponins and has antibacterial activity against Staphylococcus aureus. This study aims to test the antibacterial effectiveness of combination of celery and teak leaf cream and evaluate cream with parameters of physical tests (organoleptic, homogeneity, pH, spreadability, adhesion) and physical stability with cycling test method. This study used an experimental laboratory method by making 5 variations concentration of extracts. Celery and teak leaf extract was extracted by maceration method. Antibacterial test used the wells method. Results of the evaluation of physical tests for organoleptic test (odor: a typical combination of extracts;color: brown;shape: semi-solid), homogeneity test (homogeneous), average of pH test is 6.02, dispersion test is 4.27 cm, adhesion test is 18.42 seconds. Based on the results of this study, concluded that the combination of celery leaf and teak leaf extract can be formulated into a cream. The cream of combination of celery and teak leafs extract are bacteriostatic and has antibacterial activity with an average diameter for formulation I 13.4 mm, formulation II 9.23 mm, formulation III 16.23 mm, formulation IV 11 mm, and formulation V 12.06 mm. Keywords : Celery (Apium graveolens L.), Teak (Tectona grandis Linn.f), Antibacterial, Cream. ABSTRAK Tanaman seledri (Apium graveolens L.) dan tanaman jati (Tectona grandis Linn.f) merupakan tanaman yang mengandung flavonoid, tanin dan saponin dan memiliki aktivitas antibakteri terhadap Staphylococcus aureus. Penelitian ini bertujuan untuk menguji aktivitas antibakteri sediaan krim kombinasi ekstrak daun seledri dan daun jati serta mengevaluasi sediaan krim dengan parameter uji sifat fisik (uji organoleptik, uji homogenitas, uji pH, uji daya sebar, uji daya lekat) dan stabilitas fisik dengan metode cycling test. Penelitian ini menggunakan metode eksperimental laboratorium dengan membuat 5 variasi konsentrasi ekstrak. Ekstrak diperoleh dengan cara maserasi. Uji antibakteri dilakukan dengan metode sumuran. Hasil evaluasi sifat fisik untuk uji organoleptik (bau: khas kombinasi ekstrak;warna:coklat;bentuk:setengah padat), uji homogenitas (susunan homogen), rata-rata uji pH 6,02, daya sebar 4,27 cm, daya lekat 18,42 detik. Berdasarkan hasil penelitian disimpulkan bahwa kombinasi ekstrak daun seledri dan daun jati dapat diformulasikan menjadi sediaan krim. Sediaan krim kombinasi ekstrak daun seledri dan daun jati bersifat bakteriostatik dan memiliki aktivitas antibakteri dengan diameter rata-rata untuk formula I 13,4 mm, formula II 9,23 mm, formula III 16,23 mm, formula IV 11 mm, dan formula V 12,06 mm. Kata Kunci : Seledri (Apium graveolens L.), Jati (Tectona grandis Linn.f), Antibakteri, Krim

scholarly journals FORMULASI SEDIAAN GEL EKSTRAK ETANOL DAUN KALIANDRA (Calliandra surinamensis Benth) SEBAGAI ANTIBAKTERI

PHARMACON ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 801
Author(s):  
Yulita Ni Nyoman Tri Sukartiningsih ◽  
Hosea Jaya Edi ◽  
Jainer P. Siampa
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Physical Stability ◽  
Homogeneity Test ◽  
Adhesion Test ◽  
Cycling Test ◽  
Physical Evaluation ◽  
Before And After ◽  
Gel Preparation ◽  
Ethanol Extracts

ABSTRACT Kaliandra Leaf (Calliandra surinamensis Benth) contains flavonoid compounds, saponins and tannins which are able to inhibit the antibacterial activity. This study aims to test the antibacterial effectiveness of gel preparation of ethanol extracts of kaliandra leaf to obtain the greatest formula, and prove the Kaliandra leaf gel preparation has good physical stability. This study uses a laboratory experimental method. Gel formula is made as many as 5 formulas with variations in the concentration of 2%, 3%, 4%, 5%, and 6% of w : v. Kaliandra leaf extraction was carried out by maceration method using 96% ethanol. The antibacterial test of the ethanol extracts of Kaliandra leaf gel using the method of welling against Staphylococcus aureus bacteria produced a moderate inhibition of 7.0 ± 0.63 mm at a concentration of 6%. The physical evaluation of the preparation includes organoleptic test, homogeneity test, syneresis test, scatter power test, adhesion test, pH test, cycling test, and sterility test. All tests are carried out before and after the cycling test. The results of the study on the preparation fulfilled the physical evaluation requirements before and after the cycling test. It can be concluded that the concentration of 6% of ethanol extracts of Kaliandra leaves could be formulated as a gel preparation that is physically stable and has moderate antibacterial activity. Keywords: Kaliandra (Calliandra surinamensis Benth), Gel preparations, Staphylococcus aureus antibacterial.  ABSTRAK Daun Kaliandra (Calliandra surinamensus Benth) mengandung senyawa flavonoid, saponin dan tannin yang mampu menghambat aktivitas antibakteri. Penelitian ini bertujuan untuk menguji efektivitas antibakteri sediaan gel ekstrak etanol daun Kaliandra untuk mendapatkan formula terbesar, serta membuktikan sediaan gel daun Kaliandra mempunyai stabilitas fisik yang baik. Penelitian ini menggunakan metode ekperimental laboratorium. Formula sediaan gel dibuat sebanyak 5 formula dengan variasi konsentrasi 2%, 3%, 4%, 5%, dan 6% b/v. Ekstraksi daun Kaliandra dilakukan dengan metode maserasi menggunakan etanol 96%.  Penelitian uji antibakteri sediaan gel ekstrak etanol daun Kaliandra menggunakan metode sumuran pada bakteri staphylococcus aureus menghasilkan daya hambat yang sedang yaitu 7,0±0,63 mm pada konsentrasi 6%. Evaluasi fisik sediaan meliputi uji organoleptik, uji homogenitas, uji sineresis, uji daya sebar, uji daya lekat, uji pH, uji cycling test, dan uji sterilitas. Semua pengujian dilakukan sebelum dan sesudah cycling test. Hasil penelitian sediaan memenuhi persyaratan eveluasi fisik sebelum uji cycling test dan sesudah cycling test. Dapat disimpulkan pada kosentrasi 6% ekstrak etanol daun Kaliandra dapat diformulasi sebagai sediaan gel yang stabil secara fisik dan memiliki aktivitas antibakteri yang sedang. Kata kunci : Kaliandra (Calliandra surinamensis Benth), Sediaan Gel, Antibakteri Staphlococcous  aureus.

scholarly journals FORMULATION DEVELOPMENT AND PHYSICAL TEST OF LEAF ETHANOL EXTRACT CREAM NONI (Morinda Citrifolia L) AS A WOUND HEALER

2018 ◽  
Vol 1 (1) ◽  
pp. 20-26
Author(s):  
Anom Parmadi
Keyword(s):  
Leaf Extract ◽  
Ethanol Extract ◽  
Test Method ◽  
Homogeneity Test ◽  
Formulation Development ◽  
Physical Test ◽  
Power Test ◽  
Healing Power ◽  
Dispersion Test ◽  
Made In

Injuries are lost or damaged part of the body's tissues. Wounds are also defined as physical damage due to the opening or destruction of the skin which can cause abnormal skin function and anatomy. These plants widely used traditionally from fresh noni leaves used as a medicine for broken bones, cuts, burns and pain by boiling and drinking the juice. These studys to make use of more effective and efficient Noni leaf extract made in cream preparations as a wound medicine, so that people are easy to use. This study used an experimental research method, namely the cream formulation of noni concentrated ethanol extract of 5%, 10% and 15%. Cream preparation evaluation test included: Organoleptic, pH test, cream type test, homogeneity test, sticky power test, dispersion test and protection power test. The test method was carried out on mice by means of cream preparations applied to the back of mice which were given an incision wound. Its effectiveness is seen from the measurement of wound length for seven days to calculate the percentage of wound healing power. The results of the One Way ANOVA test showed that there were significant differences between concentrations of 5%, 10% and 15% with significant values <0.05. The percentage of healing power of noni leaf extract cream concentration of 5% = 7,69%, concentration of 10% = 17,26%, and concentration of 15% = 29,35%. The results showed that the ethanol extract of noni leaves can be made in cream dosage form and has the effectiveness to accelerate the healing of incision wounds in mice.

scholarly journals UJI EFEKTIVITAS ANTIBAKTERI SEDIAAN KRIM EKSTRAK ETANOL DAUN KEMANGI (Ocimum basilicum L.) TERHADAP Staphylococcus aureus

PHARMACON ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 661
Author(s):  
Axcel H. Tondolambung ◽  
Hosea Jaya Edy ◽  
Julianri S. Lebang
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Dosage Form ◽  
Ph Value ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Ocimum Basilicum ◽  
Homogeneity Test ◽  
Average Value ◽  
Dispersion Test

ABSTRACT Basil leaves (Ocimum basilicum  L.) contain flavonoid, saponins and tannins that have antibacterial activity. This study is aims to make basil cream then determine antibacterial activity of cream against Staphylococcus aureus. The cream  was made with five concentrations of extract 3%, 6%, 9%, 12%, 15% respectively. The extract was obtained by maceration using 96% ethanol solvent. Antibacterial test was carried out by using the well method. The results of the evaluation of physical properties for the organoleptic test and homogeneity test showed that the cream dosage form met the requirements, the average pH value is 4.92±0.27, average value of dispersion test is 3.30±0.0 cm, average value of adhesion test is 7.92±0.29 seconds. Antibacterial effectiveness test showed an average diameter value for formula I (3%) is 8.94±1.60 mm, formula II (6%) 9.46±6.85 mm, formula III (9%) 10.30±3.28 mm, formula IV (12%) 15.18±8.04 mm, formula V(15%) 16.23±4.16 mm. The statistical test proves that formula V has the best resistance  and can be concluded that the antibacterial cream dosage form of the ethanol extract of basil leaves can be formulated into a cream that is good, physically stable and can inhibit bacterial growth. Keywords: Basil Leaves (Ocimum basilicum  L.), Cream, Antibacterial. ABSTRAK Daun Kemangi (Ocimum basilicum  L.) memiliki kandungan senyawa flavonoid, saponin dan tannin yang bersifat sebagai antibakteri. Penelitian ini bertujuan untuk membuat sediaan krim ekstrak etanol daun kemangi yang stabil secara fisik dan mengetahui daya hambat krim antibakteri terhadap bakteri Staphylococcus aureus. Krim dibuat menggunakan lima variasi konsentrasi ekstrak yaitu 3%, 6%, 9%, 12%, 15%. Ekstrak diperoleh dengan cara maserasi menggunakan pelarut etanol 96%. Uji antibakteri dilakukan dengan metode sumuran. Hasil evaluasi sifat fisik untuk uji organoleptik dan uji homogenitas menunjukkan sediaan krim memenuhi persyaratan, nilai pH rata-rata 4,92±0,27 nilai rata-rata daya sebar 3,30±0,0 cm; niai rata-rata uji daya lekat 7,92±0,29 detik. Uji efektivitas antibakteri menunjukkan nilai rata-rata diameter untuk formula I (3%); 8,94±1,60 mm; formula II (6%) 9,46±6,85 mm; formula III (9%) 10,30±3,28 mm; formula IV (12%) 15,18±8,04 mm; dan formula V (15%) 16,23±4,16 mm. Uji statistika membuktikan bahwa formula V memiliki daya hambat yang paling baik dan disimpulkan bahwa ekstrak etanol daun kemangi dapat diformulasikan menjadi sediaan krim yang baik dan stabil secara fisik dan dapat menghambat pertumbuhan bakteri. Kata Kunci: Daun Kemangi (Ocimum basilicum  L.) , Krim, Antibakteri

scholarly journals Daya Antibakteri Ekstrak Daun Seledri (Apium graveolens L.) terhadap Porphyromonas gingivalis

2019 ◽  
Vol 16 (2) ◽  
pp. 53
Author(s):  
Anisa Luthfiyani ◽  
Peni Pujiastuti ◽  
Melok Aris W.
Keyword(s):  
Porphyromonas Gingivalis ◽  
Leaf Extract ◽  
Antibacterial Effect ◽  
Test Method ◽  
Apium Graveolens ◽  
Diffusion Method ◽  
Antibacterial Test

Background: celery leaves contain antibacterial substances that can be used as medicine. These contents include flavonoids, saponins and tannins. Purpose: the aim of this study was to determine the antibacterial effect of celery leaves (Apium graveolens L.) on the growth of Porphyromonas gingivalis (P. gingivalis). Methods: celery leaves were extracted by maceration method with 96% ethanol. The antibacterial test method against P. gingivalis using the well diffusion method on BHI-A media. The concentrations of celery leaf extract used in this study were 6.25%, 12.5%, 25%, 50% and 100%. Result: The results showed that celery leaves extract (Apium graveolens L.) didn’t have the ability to inhibit the growth of Porphyromonas gingivalis. Conclusion: celery leaves didn’t have antibacterial effect against Porphyromonas gingivalis in 6,25%, 12.5%, 25%, 50% and 100% concentrations.

Phytochemical and Antibacterial Action of Taro (Colocasia esculenta, Araceaea) Aqueous-Ethanolic Leaf Extract against Selected Bacterial Strains

10.17158/232 ◽  
2012 ◽  
Vol 18 (1) ◽  
Author(s):  
Judee N. Nogodula ◽  
Jessa Marie D. Draug ◽  
Maryjane S. Jamero
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Salmonella Typhimurium ◽  
Ames Test ◽  
Leaf Extract ◽  
Inhibitory Concentration ◽  
Mutagenic Activity ◽  
Standard Drug ◽  
Ethanolic Leaf Extract

Taro (Colocasia esculenta) plant is commonly available and popularly used as food and alternative medicine. To prove its medicinal value, the study explored its secondary metabolites from aqueous-ethanolic leaf extract. Specifically, this investigation aimed to classify its acute dermal toxicity and antibacterial activity, determine its Minimum Inhibitory Concentration (MIC), and identify the equipotency with the standard drug and mutagenic activity. Phytochemical screening of tannins, alkaloids, saponins, cardenolides and bufadienolides, flavonoids, polyphenol compounds and anthraquinones was performed. Five healthy female rabbits were used for toxicity test based on OECD guidelines 404. Kirby-Bauer method was employed for antibacterial activity (susceptibility and potency tests) using Methicillin-Resistant Staphylococcus aureus ATCC 43300, Clinical Isolate Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli. A two-fold agar dilution was applied for Minimum Inhibitory Concentration and Ames test was employed for direct mutagenicity assay using Salmonella typhimurium TA98. Results showed that leaf extract has no anthraquinone and it is categorized as non toxic up to allowable dose of 5000 mg/kg. The findings showed a significant difference on the mean zones of inhibition between Vancomycin and plant extract against S. aureus and between tetracycline and the extract towards E.coli. The MRSA and P. aeruginosa showed no significant differences. The MIC of extract is effective to MRSA and S. aureus at 105.26 and 50 mg/mL respectively. However, E. coli and P. aeruginosa are resistant up to the 105.26 mg/mL. Potency test revealed a non-comparability in strength between the extract and Azithromycin using Gram-negative bacteria. However, the extract showed comparable strength with the standard drug using MRSA and S. aureus. Ames test revealed a mutagenic activity using Salmonella typhimurium TA98.

scholarly journals ANTIBACTERIAL ACTIVITY TEST OF LEAVES KECOMBRANG (NICOLAIA SPECIOSA) ETHANOLIC EXTRACTS AGAINST STAPHYLOCOCCUS AUREUS

2020 ◽  
Vol 7 (2) ◽  
pp. 51-58
Author(s):  
Rinda Binugraheni ◽  
Ndaru Trisni Larasati
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Infectious Diseases ◽  
Leaf Extract ◽  
Diffusion Method ◽  
Skin Infections ◽  
Activity Test ◽  
Ethanolic Extracts ◽  
Made In ◽  
Data Result

Infectious diseases that often occur in humans are skin infections. One of the bacteria that cause infection in humans is Staphylococcus aureus. One of the plants used as an ingredient in traditional medicine and used as an anti-bacterial is a kecombrang plant. This research aims to test the antibacterial activity of leaf extract of kecombrang against Staphylococcus aureus. Kecombrang leaf obtained from Pandeglang Banten. Kecombrang leaves extracted with a maceration method using 96% ethanol solvent. Extracts of leaves are made in a various concentrations (100%, 75%, 50%, and 25%). The antibacterial activity test was performed by using the diffusion method to find out the large zone of diameter are formed to inhibiting Staphylococcus aureus bacteria.The results of the antibacterial activity of the leaves extract of kecombrang to Staphylococcus aureus at 25%, 50%, 75% and 100% concentrations respectively were 12.67 mm, 14.33 mm, 15.33 mm, and 17.00 mm. The data result showed, that leaf extract of kecombrang with 100% concentration had the largest inhibiting zone diameter of 17.00 mm, among other concentrations. This suggests that kecombrang leaf extract have antibacterial activity against Staphylococcus aureus.

scholarly journals Hand Sanitizer Ekstrak Daun Pucuk Idat (Cratoxylum glaucum) sebagai Antibakteri Staphylococcus aureus dan Escherichia coli

2020 ◽  
Vol 16 (2) ◽  
pp. 227
Author(s):  
Megawati Ayu Putri ◽  
Marhan Ebit Saputra ◽  
Ike Nur Amanah ◽  
Siska Musiam ◽  
Verry Andre Fabiani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Skin Irritation ◽  
Test Results ◽  
Antibacterial Test ◽  
E Coli ◽  
Hand Sanitizer ◽  
Carboxymethyl Cellulose Sodium

<p><span id="docs-internal-guid-2cebd816-7fff-d997-4dfd-796d046d0d3b"><span>Daun pucuk idat merupakan tanaman lokal Bangka Belitung yang memiliki aktivitas antibakteri terhadap </span><span>Staphylococcus aureus </span><span>dan</span><span> Escherichia coli.</span><span>Sebagai upaya pemanfaatan potensi lokal maka dibuat suatu produk sediaan gel </span><span>hand sanitizer</span><span> yang praktis dan ekonomis. </span><span>Ekstrak daun pucuk idat diperoleh dengan metode maserasi menggunakan pelarut aseton. Ekstrak yang dihasilkan diformulasikan dalam berbagai konsentrasi ekstrak daun pucuk idat (40%, 60%, 80%, dan 100%) dengan penambahan </span><span>carboxymethyl cellulose sodium</span><span> (</span><span>CMC-Na) 0,25 g, gliserin 1 mL, propilenglikol 0,5 mL serta akuades        10 mL. Setiap formulasi gel </span><span>handsanitizer</span><span> dilakukan pengujian stabilitas fisik meliputi uji organoleptis, uji homogenitas, uji daya sebar, uji pH, uji iritasi kulit serta uji aktivitas antibakteri.</span><span> </span><span> Hasil uji organoleptik menunjukkan bahwa semua konsentrasi </span><span>hand sanitizer </span><span>berbentuk gel, berwarna kuning muda, berbau dan homogen. Uji pH dan daya sebar gel</span><span> hand sanitizer</span><span> telah memenuhi ketentuan SNI No. 06-2588. Pengujian iritasi kulit tidak menimbulkan kemerahan, gatal-gatal dan tidak mengakibatkan kulit menjadi kasar pada punggung tangan sukarelawan. Berdasarkan uji sifat fisik gel</span><span> hand sanitizer </span><span>yang dihasilkan memiliki sifat fisik yang serupa dengan gel </span><span>hand sanitizer </span><span>komersial. Hasil uji antibakteri hand sanitizer ekstrak daun pucuk idat terhadap </span><span>E. coli</span><span> dengan konsentrasi ekstrak daun pucuk idat 40%, 60%, 80%, dan 100% diperoleh diameter zona hambat berturut-turut sebesar 11,5 mm, 17,4 mm, 19,3 mm dan 19,3 mm, sedangkan pada bakteri </span><span>S. aureus</span><span> diperoleh diameter zona hambat berturut-turut sebesar 17,1 mm, 24,2 mm, 27,4 mm, dan  18 mm. Hasil uji antibakteri menunjukkan bahwa sediaan gel </span><span>hand sanitizer </span><span>ekstrak aseton daun pucuk idat memiliki aktivitas antibakteri terhadap bakteri </span><span>E. coli </span><span>dan </span><span>S. aureus</span><span>.</span></span></p><p><span><span><span id="docs-internal-guid-50d6ff2c-7fff-2e66-45f1-73cf49e6527a"><strong>Hand Sanitizer of Leaves Extract of Cratoxylum glaucum as Antibacterial of Staphylococcus aureus and Escherichia coli. </strong><span>Cratoxylum glaucum</span><span> leaves is local plants of Bangka Belitung which have antibacterial activity against </span><span>S. aureus</span><span> and </span><span>E. coli</span><span>. As an effort to explore local potential, a practical and economical gel hand sanitizer product was prepared. </span><span>Cratoxylum glaucum</span><span> leaves extract was obtained by maceration method using acetone. The extract was formulated in various concentrations of </span><span>Cratoxylum glaucum</span><span> leaf extract (40%, 60%, 80%, and 100%) with the addition of 0.25 g carboxymethyl cellulose sodium (CMC-Na), 1 mL glycerin, 0.5 mL propylene glycol and 10 mL distilled water. Each concentration of the hand sanitizer gel was tested for physical stability including organoleptic, homogenity, spreadability, pH, skin irritation and antibacterial activity test. The organoleptic test results showed that all concentrations of hand sanitizers formed gel with light yellow color, smelly odor and homogeneous form. The analysis of pH and spreadability of hand sanitizer gel has fulfilled SNI No. 06-2588. The skin irritation testing does not cause redness, itching, and rough skin on the back of volunteer hands. Based on the physical properties test, the resulting hand sanitizer gel has physical properties similar to commercial hand sanitizer gel. The results of the antibacterial test of hand sanitizer against </span><span>E. coli</span><span> with </span><span>Cratoxylum glaucum</span><span> leaf extract concentrations of 40%, 60%, 80%, and 100% obtained the diameter inhibitory zones of 11.5 mm, 17.4 mm, 19.3 mm, and 19.3 mm, respectively. Meanwhile, the results of the antibacterial test of hand sanitizer against </span><span>S. aureus</span><span> bacteria with concentration of 40%, 60%, 80%, and 100% shows that the inhibition zone diameters were 17.1 mm, 24.2 mm, 27.4 mm, and 18 mm, respectively. The antibacterial test results shows that the gel hand sanitizer prepared has antibacterial activity against </span><span>E. coli</span><span> and </span><span>S. aureus</span><span> bacteria.</span></span></span></span></p>

scholarly journals Aktivitas Antibakteri Ekstrak Daun Jarak Pagar (Jatropha Curcas L) Terhadap Staphylococcus Epidermidis Dan Staphylococcus Aureus Secara In Vitro

2018 ◽  
Vol 7 (1) ◽  
Author(s):  
Susilo Yulianto ◽  
Sunarmi Sunarmi
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Jatropha Curcas ◽  
Staphylococcus Epidermidis ◽  
Leaf Extract ◽  
Inhibitory Zone ◽  
Zone Diameter ◽  
Jatropha Curcas L ◽  
Inhibitory Power ◽  
And Staphylococcus Aureus

Abstract: Jatropha Leaves, Staphylococcus Epidermidis, Staphylococcus Aureus. Jatropha curcas L leaves contain tannins, saponins and flavonoids. According to some studies, tannins, saponins and flavonoids have antibacterial power. Based on these indications, it is important to do research on antibacterial potency of jatropha leaf extract (Jatropha curcas L) against Staphylococcus epidermidis and Staphylococcus aureus bacteria. The aim of this research is to know Antibacterial Activity of Jatropha curcas L Extract on Staphylococcus epidermidis and Staphylococcus aureus". This research. An experimental study by testing the antibacterial activity of leaf extract and jatropha result of maceration, infundation and reflux as measured by Minimal Inhibitory Concentration (KHM) on growth of Staphylococcus epidermidis and Staphylococcus aureus bacteria. From the results of the research, it was found that the extract of the reflux was brownish green and the characteristic of jatropha leaves. The amount of extract produced was 17.03 g, so the yield was 17.03%. Jatropha leaf extract of maceration has no inhibitory power to S. epidermidis in 4 series concentrations starting from 3.125 ppm to 25.000 ppm. Inhibitory zones begin to appear at concentrations of 50,000 ppm and increase at concentrations of 100,000 ppm. The drag zone diameter in the Jatropha curcas extract of the reflux showed the inhibitory power in all series of concentrations. The smallest inhibitory zone was shown at a concentration of 3,125 ppm (9.33 mm) and increased with increasing concentrations. The diameter of the inhibit zone was greatest at 100,000 ppm concentrations of 22.67 mm. Description of inhibitory zone diameter of methanol leaf extract of jatropha result of maceration and reflux to S. epidermidis. The conclusion of this research is extract of reflux result giving bigger resistance than maceration extract to S. epidermidis and S. aureus. Methanol extract of jatropha from the maceration and reflux have strong antibacterial activity against S. epidermidis and S. aureus but still under Klindamycin.

scholarly journals Green Synthesis of Silver Nanoparticles Using Moringa Oleifera Leaf Extract, Tamarindus Indica Fruit Extract and Assessement of Antibacterial Activity Against Staphylococcus Aureus

2021 ◽  
Author(s):  
Shirisha A ◽  
ANUMOLU VIJAYA KUMAR ◽  
Laxman Chatlod R ◽  
Shashi Kumar M ◽  
Krishnaiah N ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Silver Nitrate ◽  
Leaf Extract ◽  
Moringa Oleifera ◽  
Diffusion Method ◽  
Tamarindus Indica ◽  
Fruit Extract

Abstract The present study mainly deals with the green synthesis, characterization and evaluation of antibacterial properties of silver nanoparticles (AgNPs) synthesized by using the leaf extract of Moringa oleifera and fruit extract of Tamarindus indica. In this study for synthesis of silver nanoparticles different ratios of 1mM silver nitrate and Moringa oleifera leaf extract i.e, 95:5, 90:10 and 85: 15 was taken in conical flask and kept for one 1 hr at 25 0 c on magnetic stirrer, out of which 90:10 ratio was selected for further study based on highest peak, good size and stability. Tamarindus indica fruit extract was added to silver nitrate solution till the colour of the solution changes from light brown to chocolate brownish colour. The synthesized silver nanoparticles were characterized by UV-Visible spectroscopy, Zeta potential, size distribution by intensity. The absorption spectrum of the silver nano solution prepared by using Moringa oleifera and Tamarindus indica fruit extract showed a surface plasmon absorption band with maximum of 420 nm and 430 nm respectively indicating the presence of silver nanoparticles. The zeta value of silver nanoparticles synthesized from Moringa oleifera and Tamarindus indica fruit extract was -12.5 mV and -15.5 mV, size of 110.2 nm and 130.2 nm respectively. The antibacterial efficacy of nanosilver was checked by agar well diffusion method, and the silver nanoparticles showed effective antibacterial activity against Staphylococcus aureus.

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