scholarly journals UJI TOKSISITAS AKUT INFUSA BUAH MENGKUDU (Morinda citrifolia L.) PADA Artemia salina MENGGUNAKAN METODE BRINE SHRIMP LETHALITY TEST (BSLT)

2019 ◽  
Vol 2 (2) ◽  
pp. 75
Author(s):  
Nuri Mutammimah ◽  
Bilal S A Santoso
Keyword(s):  
Brine Shrimp ◽  
Artemia Salina ◽  
Negative Control ◽  
Test Method ◽  
Morinda Citrifolia ◽  
Control Group ◽  
Control Group Design ◽  
Brine Shrimp Lethality ◽  
Group Design ◽  
Post Test

ABSTRACT Mengkudu fruit (Morinda citifolia L.) is one of plants which has been known and used by indonesian society as traditional medicine. This plant contains alkaloid, phenol, steroid, and flavonoid which can cause toxicity. The aim of this study was to know about acute toxicity of mengkudu fruit infusions using brine shrimp lethality test method which will be indicated by LC50 value. This research was experimental research using post test only control group design that used negative control and 5 concentrations of infusion those are 1000 mg/L, 500 mg/L, 100 mg/L, 50 mg/L, and 10 mg/L. Death of larvae observed 24 hours after giving the infusiona. LC50 of mengkudu fruit infusion is 62,3 mg/L. The result of this study showed that mengkudu fruit infusions cause toxic effect. Keywords : Noni Fruit, Toxycity, Brine Shrimp Lethality Test, LC50   ABSTRAK Buah mengkudu (Morinda citrifolia L.) merupakan salah satu tanaman yang banyak dikenal dan digunakan oleh masyarakat sebagai obat tradisional. Tanaman ini mengandung senyawa alkaloid, fenol, steroid, dan flavonoid yang dapat menyebabkan tosiksitas dalam jumlah pemakaian tertentu. Penelitian ini bertujuan untuk mengetahui toksisitas akut infusa buah mengkudu dengan menggunakan metode Brine Shrimp Lethality Test yang akan ditunjukkan oleh nilai LC50. Penelitian ini termasuk penelitian ekperimental dengan post test only control group design. Uji ini menggunakan kontrol negatif dan 5 konsentrasi infusa yaitu 1000 mg/L, 500 mg/L, 100 mg/L, 50 mg/L, 10 mg/L. Kematian larva diamati 24 jam setelah pemberian infusa. Hasil penelitian menunjukkan nilai LC50 infusa buah mengkudu sebesar 62,3 mg/L. Kesimpulan dari penelitian ini adalah infus buah mengkudu bersifat toksik. Kata kunci: Toksisitas, Mengkudu, Brine Shrimp Lethality Test, LC50

scholarly journals Komposisi metabolit sekunder dan uji toksisitas fraksi etil asetat daun leban (Vitex pinnata Linn)

2021 ◽  
Vol 10 (1) ◽  
pp. 59
Author(s):  
Sabarudin - Sabarudin ◽  
Dewi Kurniasih
Keyword(s):  
Acute Toxicity ◽  
Brine Shrimp ◽  
Freeze Drying ◽  
Artemia Salina ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
X 24 ◽  
Test Control

ABSTRAK   Telah dilakukan skrining dan uji toksisitas tehadap ekstrak daun Leban (Vitex Pinnata Linn. Tujuan penelitian ini adalah untuk mengetahui komposisi kandungan senyawa metabolik sekunder dan potensi toksisitas akut ektrak daun muda. Penelitian ini adalah penelitian eksperimental dengan Only Post Test Control Group Design, ektraksi diawali dengan pengambilan sampel dari sekitar perkebunan penduduk di Desa Pudak. Pengolahan menjadi simplisia serbuk dengan melalui tahap sortasi basah, pencucian, pengeringan dan sortasi kering. Sediaan simplisia diekstraksi dengan cara masserasi direndam dalam pelarut n-heksana Merk selama 3 x 24 jam. Maserat atau filtrat selanjutnya dipisahkan menggunakan kaca corong pisah dan kertas saring, kemudian di uapkan dengan alat vacuum rotari evaporator sehingga diperoleh ekstrak mentah. Ektrak di fraksinasi berturut-turut menggunakan pelarut pertolium eter, etil asetat dan  metanol dan dikeringkan dengan metode pengeringan beku (freeze drying). Terhadap ekstrak dilakukan identifikasi untuk mengetahui kandungan senyawa metabolit sekunder. Selanjutnya setiap ektrak dilakukan uji toksisitas menggunakan metode Brine Shrimp Lethality Test (BSLT), dengan mengamati berapa jumlah larva udang (Artemia salina Leach) yang mati setelah 24 jam diberikan bahan uji. Hasil penelitian menunjukkan bahwa didalam ekstrak terdapat kandungan triterpena atau steroid, saponin dan flavonoid serta tidak ditemukan alkaloid. Uji toksisitas memberikan informasi bahwa hanya fraksi etil asetat menunjukkan ketoksikan akut. Analisis probit hasil pengamatan uji toksisitas memberikan hasil perhitungan dengan nilai  LC50 = 218 ppm, dengan kesimpulan daun vitex pinnata Linn memiliki toksisitas akut stergolong sedang.       ABSTRACT   Screening and toxicity tests have been carried out on Leban leaf extract (Vitex Pinnata Linn. The purpose of this study was to determine the composition of secondary metabolic compounds and the acute toxicity potential of young leaf extract. This study was an experimental study with Only Post Test Control Group Design, the extraction started with sampling from around the resident's plantations in Pudak Village Processing into powder simplicia by going through the stages of wet sorting, washing, drying and dry sorting Simplicia preparations are extracted by means of masseration soaked in n-hexane solvent Brand for 3 x 24 hours Maserate or filtrate is then separated using a separating funnel and filter paper, then evaporated using a vacuum rotary evaporator to obtain the crude extract.The extract was fractionated successively using pertolium ether, ethyl acetate and methanol as solvents and dried by freeze drying method. d identification to determine the content of secondary metabolites. Furthermore, each extract was tested for toxicity using the Brine Shrimp Lethality Test (BSLT) method, by observing how many shrimp larvae (Artemia salina Leach) died after 24 hours of being given the test material. The results showed that the extract contained triterpenes or steroids, saponins and flavonoids and no alkaloids were found. Toxicity test provided information that only the ethyl acetate fraction showed acute toxicity. Probit analysis of the results of the observation of the toxicity test gave the calculation results with a value of LC50 = 218 ppm, with the conclusion that the leaves of Vitex pinnata Linn have moderate acute toxicity.    

scholarly journals Skrining fitokimia dan uji toksisitas ekstrak daun balik angin (Mallotus Sp) terhadap larva Artemia salina Leach dengan metode brine shrimp lethality test (BSLT)

2017 ◽  
Vol 2 (2) ◽  
pp. 77
Author(s):  
Anderson Arnold Aloanis ◽  
Fahriana Fahriana ◽  
Haryadi Haryadi
Keyword(s):  
Acute Toxicity ◽  
Brine Shrimp ◽  
Artemia Salina ◽  
Control Group ◽  
Control Group Design ◽  
Brine Shrimp Lethality ◽  
Regular Treatment ◽  
Post Test ◽  
Young Leaves ◽  
Ethanol Extracts

One of the plants that are used to regular treatment is behind the wind (Mallotus sp.). Traditionally this is used for the relief of pain and increase stamina by way of boiling the seven pieces of young leaves and drink once a day in the morning. This research aims to know the acute toxicity of ethanol extracts of leaves behind the wind (Mallotus sp) against larvae of Artemia salina Leach BSLT method. The research method used is qualitative analysis of chemical compound and Brine Shrimp Lethality Test (BSLT) method with post-test-only control group design. The results of research on ethanol extracts of leaves behind the wind showed the value of LC50 of 32.061 ppm, so can be said of ethanol extracts of leaves behind the wind on this experiment of acute toxicity has on the larvae of Artemia salina Brine Shrimp method using Leach Lethality Test (BSLT).

scholarly journals Pengaruh E-Modul Mata Pelajaran Video Editing Berbasis Model Pembelajaran Project based Learning Terhadap Hasil Belajar Siswa SMK

2018 ◽  
Vol 7 (2) ◽  
pp. 176
Author(s):  
Wahyu Restu Aji . ◽  
Dr. Dewa Gede Hendra Divayana, S.Kom., M . ◽  
Dr. Ketut Agustini, S.Si, M.Si. .
Keyword(s):  
Positive Response ◽  
Learning Model ◽  
Project Based Learning ◽  
Test Method ◽  
Control Group ◽  
Video Editing ◽  
Learning Method ◽  
Control Group Design ◽  
Group Design ◽  
Post Test

Tujuan penelitian ini untuk mengetahui (1) pengaruh penerapan E-Modul mata pelajaran Video Editing berbasis model pembelajaran Project Based Learning terhadap hasil belajar siswa kelas XI Multimedia di SMK TI Bali Global Singaraja, (2) Untuk mengetahui respon siswa kelas XI Multimedia di SMK TI Bali Global Singaraja terhadap pengaruh penerapan E-Modul mata pelajaran Video Editing berbasis model pembelajaran Project Based Learning. Jenis penelitian ini adalah eksperimen semu dengan rancangan Post Test Only Control Group Design. Pengumpulan data dilakukan dengan metode tes pilihan ganda untuk mengukur ranah kognitif. Data hasil belajar dianalisis melalui uji prasyarat yaitu uji normalitas dan homogenitas dengan hasil 2 kelompok berdistribusi normal dan homogen, dilanjutkan dengan uji hipotesis yang berarti terdapat pengaruh yang signifikan dalam penggunaan E-Modul mata pelajaran Video Editing berbasis model pembelajaran Project Based Learning terhadap hasil belajar siswa. Sedangkan untuk respon menggunakan metode angket. Hasil analisis angket E-Modul mata pelajaran Video Editing berbasis model pembelajaran Project Based Learning diketahui 67% respon sangat positif, 33% respon positif. Kata Kunci : E-Modul, Project Based Learning, hasil belajar, dan respon siswa The objectives of this study is to find out (1) the effect of applying e-model on video editing lesson based Project Based Learning method on XI grade of Multimedia at SMK TI Global Singaraja. (2) The students' respondent on the XI grade of Multimedia at SMK TI Global Singaraja toward the affect of e-model video editing lesson based project based learning method. This study is quasi experimental with used Post Test Only Control Group Design. Data collection was done using Objective Test Method to measure the cognitive domain. The result of the data was analyzed through prerequisite test which is normality and homogeneity test with the result 2 groups normally distributed and homogeneous, continued with hypothesis test which means there was a significant effect between using e-model video editing lesson based Project Based Learning model towards the students' learning result. While for the respondent was using questionnaire method. The analysis result of e-modul video editing lesson based Project Based Learning model questionnaire is known 67% on very positive response, 33% on positive response. keyword : E-Module, Project Based Learning, learning outcomes, students’ respond

scholarly journals Uji Efektivitas Sediaan Hand Sanitizer Kombinasi Ekstrak Daun Kemangi (Ocimum Sanctum L) Dan Ekstrak Kulit Jeruk Purut (Citrus Hystrix)

2016 ◽  
Vol 1 (2) ◽  
Author(s):  
Indri Kusuma Dewi ◽  
Bambang Yunianto
Keyword(s):  
Confidence Level ◽  
Negative Control ◽  
Ocimum Sanctum ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Hand Sanitizer ◽  
Post Test ◽  
Test Control ◽  
Optimal Formula

Absctract : Antiseptic Activity, Kemangi Leaf- Cytrus Hystrix Leather, Gel Antiseptic (Hand Sanitizer). It is known kemangi leaf had contained saponins, flavonoids and tannins that benefit as antibacterial, while in research by Susanti, 2010 had proven that leather of Citrus hystrix contained essential oil as antibacterial and used as antiseptic in pharmaceutical case. So, to get the optimal formula as hand sanitizer or antiseptic gels used combination two extracts kemangi leaf and Citrus hystrix leather. This research is an experimental research with Post Test Control Group Design to analyze antiseptic activity using the Replica methods with to account the number of colonies of microorganism. Statistic test to the number of colonies of microorganism with Anova Test one way that confidence level 95% and obtained significance 0,000 the result P < 0,05 and continued to Tukey HSD test that confidence level 95%. The result statistic tes showed that have significan differences between negative control with the formula of gel antiseptic combination extract of kemangi leaf and extract Cytrus hystrix leather, so this formula have activity as antiseptic. The most effective variation concentration is concentration extract of kemangi leaf 75% and extract Cytrus hystrix leather 25%.

scholarly journals UJI EFEKTIFITAS BIOLARVASIDA EKSTRAK ETANOL BUAH LAMPESU (Baccaurea lanceolata) TERHADAP LARVA INSTAR III Culex quinquefasciatus

2020 ◽  
Vol 3 (3) ◽  
pp. 7
Author(s):  
Dea Alfani Nandjan
Keyword(s):  
Culex Quinquefasciatus ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Chemical Insecticide ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Infection Disease

Filariasis is chronic infection disease caused by worm and carried by Culex quinquefasciatus mosquito. One of theways to controlling mosquitoes vectors are use biolarvacide or chemical insecticide. Chemical insecticide causingresistance Culex quinquefasciatus larvae and toxic for human. This research aim to determine the biolarvacideeffectiviteness of ethanol extract lampesu fruit (Baccaurea lanceolata) to Culex quinquefasciatus larvae instars III.This study was true experimental with a Post test-only control group design. This study used 700 larvae instars III ofCulex quinquefasciatus divided into 7 groups consentration of 0,2%, 0,4%, 0,6%, 0,8% and 1%, the positive control(abate) and negative control (aquadest). The observation was did after treatment in 3 hours, 6 hours, 12  hours and 24hours. The experiment is replicated four times. At 24 hours exposure concentration of 0,6% the test larvae mortalityreached 38% and at concentration of 1% the test larvae mortality reached 30%. In this stuy the concentration of1,531 % was effective to kill larvae with of 50% mortality(LC50) and concentration of 10,729 % was effective to killlarvae with of 90% mortality(LC90). Ethanol Ekstract of Lampesu Fruit (Baccaurea lanceolata) not effective asbiolarvacide ofCulex quinquefasciatus larvae instars III.

scholarly journals Uji daya hambat getah kulit buah pisang goroho (Musa acuminafe L.) terhadap pertumbuhan Staphylococcus aureus

e-GIGI ◽  
2017 ◽  
Vol 5 (2) ◽  
Author(s):  
Bayu K. Rante ◽  
Youla A. Assa ◽  
Paulina N. Gunawan
Keyword(s):  
Staphylococcus Aureus ◽  
Inhibitory Effect ◽  
Negative Control ◽  
Banana Peel ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Paper Disk ◽  
Chronic Inflammatory Condition ◽  
Post Test

Abstract: Abscess is a chronic inflammatory condition formed by localized infections. In oral cavity abscess, the causal bacteria oftenly found is Staphylococcus aureus. Goroho banana (Musa acuminafe L.) is a typical plant in North Sulawesi. The sap of goroho banana peel contains phytochemicals inter alia flavonoids, saponins, and tannins. This study was aimed to find out whether the sap of goroho banana peel (Musa acuminafe L.) had an inhibitory effect on the growth of Staphylococcus aureus. This was an experimental laboratory study with a post-test only control group design. A modified Kirby-Bauer using paper disk was used as the analytical laboratory method. We used 100% goroho banana peel sap, clindamycin antibiotics as the positive control, and CMCs as the negative control. Staphylococcus aureus bacteria was obtained from the Laboratory of Microbiology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University, Manado. The results showed that the mean diameter of inhibition zones of goroho banana peel sap was 10.9 mm and was classified as strong inhibition. Conclusion: Goroho banana peel sap had a strong inhibitory effect on the growth of Staphylococcus aureus.Keywords: goroho banana peel sap (Musa acuminafe L.), Staphylococcus aureus, inhibition zone Abstrak: Abses merupakan suatu kondisi inflamasi kronik yang terbentuk dari hasil infeksi yang terlokalisasi. Salah satu bakteri penyebab abses yang sering ditemukan pada rongga mulut ialah Staphylococcus aureus. Pisang goroho merupakan salah satu tanaman khas Sulawesi Utara. Getah kulit buahnya memiliki kandungan fitokimia seperti flavonoid, saponin, dan tanin. Penelitian ini bertujuan untuk mengetahui daya hambat getah kulit buah pisang goroho (Musa acuminafe L.) terhadap pertumbuhan bakteri Staphylococcus aureus. Jenis penelitian ini ialah eksperimental laboratorik dengan post test only control group design. Metode yang digunakan ialah modifikasi Kirby-Bauer dengan menggunakan paper disk. Konsentrasi getah buah pisang goroho yang digunakan yaitu 100%, kontrol positif menggunakan antibiotik klindamisin, dan kontrol negatif menggunakan CMC. Bakteri Staphylococcus aurues diambil dari stok bakteri murni Laboratorium Mikrobiologi Fakultas MIPA Unsrat Manado. Hasil penelitian menunjukkan diameter rerata zona hambat dari getah kulit buah pisang goroho yang terbentuk ialah 10,9 mm dan digolongkan sebagai zona hambat kuat. Simpulan: Getah kulit buah pisang goroho memiliki daya hambat yang kuat terhadap pertumbuhan Staphylococcus aureus.Kata kunci: getah kulit buah pisang goroho (Musa acuminafe L.), Staphylococcus aureus, zona hambat

scholarly journals Uji Daya Tolak Lilin Aromaterapi Minyak Atsiri Serai (Cymbopogon citratus) terhadap Nyamuk Aedes aegypti

2020 ◽  
pp. 21-28
Author(s):  
Anizah Paramitha Dewi ◽  
Novyan Lusiyana
Keyword(s):  
Aedes Aegypti ◽  
Cost Effective ◽  
Negative Control ◽  
Control Group ◽  
Cymbopogon Citratus ◽  
Control Group Design ◽  
Treatment Groups ◽  
Group Design ◽  
Post Test ◽  
Lemongrass Essential Oil

Aromatherapy candle is a safe, effective, and cost-effective repellent. It has a potential repellent effect particularly on mosquitoes. Lemongrass is a potential plant as a natural repellent for Aedes aegypti. The purpose of this study was to determine the repellency effect of lemongrass essential oils against Ae. aegypti. This study was an experimental study with a post-test only with control group design, which was divided into six groups (one negative control and five treatment groups 9%, 10%, 11%, 12%, and 13%) with four repeats. Each group consisted of 20 Ae. aegypti female mosquitoes, so the total number of mosquitoes used was 480. Tested mosquitos were exposed to aromatherapy candles for one hour, and their repellency observed every 15-minute intervals. The average of repellency effect of the negative control and 5 treatment groups at minute 60 were 7.5%; 0%; 33.33%; 66.67%; 66.67%; and 100% respectively. We conclude that the aromatherapy candle of lemongrass essential oil was effective as a 100% repellency effect against Ae. aegypti at a concentration of 13%.

scholarly journals Pengaruh Pemberian Ekstrak Rosella (Hibiscus sabdariffa Linn) Terhadap Kadar Malondialdehid dan Aktivitas Katalase Tikus yang Terpapar Karbon Tetraklorida

2015 ◽  
Vol 4 (3) ◽  
Author(s):  
Zuraida Zuraida ◽  
Eti Yerizel ◽  
Eliza Anas
Keyword(s):  
Vitamin C ◽  
Negative Control Group ◽  
Organ Damage ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Hibiscus Sabdariffa ◽  
Control Group Design ◽  
Group Design ◽  
Post Test

Abstrak Pemberian rosella (Hibiscus sabdariffa Linn) diharapkan melindung hepar tikus dari kerusakkan akibat stres oksidatif pada keracunan karbon tetraklorida (CCl 4). Senyawa yang sering dijadikan petunjuk adanya kerusakan tersebut adalah malondialdehid (MDA). Rosella mengandung vitamin C, flavonoid, polifenol dan beta karoten. Tujuanpenelitian ini adalah menentukan pengaruh pemberian ekstrak rosella (Hibiscus sabdariffa Linn) terhadap MDA dan aktivitas katalase tikus yang terpapar CCl 4. Ini adalah penelitian eksperimental dengan desain Post test Only Control Group Design. Sampel 24 ekor tikus Strain Wistar berumur 2-3 bulan, berat 150-200 gr. Sampel diambil secara acakdan dibagi 4 kelompok terdiri dari kelompok kontrol negatif, kontrol positif (CCl 4), perlakuan 1 (CCl 4 dan ekstrak rosella 250 mg/kg bb) dan perlakuan 2 (CCl4  dan ekstrak rosella 500 mg/kg bb). Pemberian CCl 4secara oral dosis tunggal, setelah 24 jam kemudian diberi ekstrak rosella secara oral selama 14 hari. Data dianalisis dengan uji Anova, tingkatkepercayaan 95%.Pemberian ekstrak rosella secara statistik didapatkan perbedaan yang signifikan rerata kadar MDA dan katalase antar kelompok (p < 0,05). Disimpulkan bahwa ekstrak rosella dapat menurunkan kadar MDA dan meningkatkan aktivitas katalase tikus yang terpapar CCl 4. Kata kunci: karbon tetraklorida, MDA, katalase, rosella Abstract Administering roselle (Hibiscus sabdariffa Linn) is expected to protect rat liver from damage caused by oxidative stress in CCl4 poisoning. Rosella contains vitamin C, flavonoids, polyphenol and beta carotene. Compounds which was often used as marker of the damage caused by free radicals wa MDA. The objective of this study was to determine the effect of extracts of roselle (Hibiscus sabdariffa Linn) on MDA and catalase activity of rats exposed to CCl4. Experimental research design with Post test Only Control Group Design. Samples of 24 male Wistar Strain rats were 2-3 months old. weighing 150-200 gr. Samples were taken at random and divided into 4 groups consisting of a negative control group, positive control (CCl4), treatment 1 (CCl4 and roselle extract 250 mg / kg bw) and treatment 2 (CCl4 and roselle extract 500 mg / kg bw). CCl4 was given a single dose orally, after 24 hours, the subjects were given rosella extract orally for 14 days. Data were analyzed by ANOVA with a confidence level of 95%. Rosella extract obtained statistically significant differences of MDA and catalase levels among groups (p <0.05). It can be concluded that the rosella extract can reduce levels of MDA and increase the activity of catalase mice exposed to CCl4. From the research it can be concluded that the rosella extract can reduce levels of MDA and increas the activity of catalase mice exposed to CCl4. It is needed further research on the toxicity of extracts of rosella and organ damage caused.Keywords: carbon tetrachloride, MDA, catalase, rosella

scholarly journals Pengaruh Model Pembelajaran Probing-Prompting Berbantuan Media Sederhana Terhadap Hasil Belajar IPA

2018 ◽  
Vol 2 (3) ◽  
pp. 321
Author(s):  
Sherina Surya Sahayu ◽  
I nyoman Jampel ◽  
I nyomn Laba jayanta
Keyword(s):  
Learning Outcomes ◽  
Learning Model ◽  
Test Method ◽  
Control Group ◽  
Class Iii ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Quasi Experimental ◽  
Receive Treatment

This study aims at determining the differences in learning outcomes between groups of students who received treatment of probing-prompting models with simple media assisted with groups of students who did not receive treatment of probing-prompting model with simple media assisted on science subjects. This study is quasi-experimental research using non-equivalent design post-test only control group design. The population of this study is the entire class III SDN in Gugus III Banjar District. The sample of this research is SDN 3 Dencarik which amounted to 20 people as experiment group and SDN 2 Dencarik which amounted to 25 people as control group. The methods of data collection are using the test method that was done through multiple choice instrument test of science learning outcomes. The hyphotesis analysis was done by using t-test asissted with SPSS 20. The results showed that there were differences of learning outcomes in the group of students who were given the treatment of probing-prompting model with simple media assisted with the group of students who were not given treatment of probing-prompting model with simple media-assisted learning in the science lessonThus, the probing-prompting learning model with simple media assisted affects the learning outcomes of Science subject on grade III students in Gugus III Banjar District, Buleleng Regency, Year 2017/2018. The suggestion of this research is that it can be used as a guide in further research about simple media assisted probing-prompting learning model especially on IPA.

scholarly journals Efektifitas Ekstrak Morinda Citrifolia Dalam Mensterilisasi Limbah Infeksius B3 Di Puskesmas Kabere Kabupaten Enrekang

2020 ◽  
Vol 3 (1) ◽  
pp. 55
Author(s):  
Hetti Hetti ◽  
Rahmi Amir
Keyword(s):  
Health Center ◽  
Morinda Citrifolia ◽  
Control Group ◽  
Average Amount ◽  
District Health ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Quasi Experimental ◽  
Infectious Waste

ABSTRAKHETTI, Efektifitas Ekstrak Morinda Citrifolia Dalam Mensterilisasi Limbah Infeksius B3 Di Puskesmas Kabere Kabupaten Enrekang, dibimbing oleh RAHMI AMIR dan AMIR PATINTINGAN.Jenis penelitian ini adalah quasi eksperimental dengan rancangan desain post test only control group design. Tujuan penelitian ini adalah untuk mengetahui efektifikas ekstrak morinda citrifolia dalam mensterilisasi limbah infeksius B3 di Puskesmas Kabere Kabupaten Enrekang sebelum dan setelah perlakuan penambahan ekstrak morinda citrifolia dengan berbagai konsentrasi yaitu 30%, 50%, dan 70%, dimana dilakukan tiga kali percobaan.Berdasarkan hasil yang didapatkan dalam penelitian ini terdapat kuman pada limbah infeksius (kain kasa) B3 sebelum perlakuan jumlah koloni bakteri pada limbah infeksius B3 Tidak Bisa Untuk Dihitung (TBUD) dan setelah perlakuan sterilisasi penambahan ekstrak morinda citrifolia terjadi daya hambat terhadap koloni bakteri dengan tiga kali percobaan. Perlakuan penambahan konsentrasi 30% ekstrak morinda citrifolia rata-rata jumlah daya hambat koloni bakteri yang diperoleh sebanyak 0,1 mm, pada konsentrasi 50%  ekstrak morinda citrifolia rata-rata jumlah daya hambat koloni bakteri yang diperoleh sebanyak 0,08 mm, sedangkan pada konsentrasi 70% ekstrak morinda citrifolia rata-rata jumlah daya hambat koloni bakteri yang diperoleh sebanyak 0,58 mm. Dosis konsentrasi ekstrak morinda citrifolia dalam penelitian ini adalah semakin banyak jumlah konsentrasi yang ditambahkan maka semakin besar daya hambat yang diberikan.Kata Kunci: Limbah Infeksius B3, Ekstrak Morinda CitrifoliaABSTRACTHETTI, the Effectiveness of Morinda Citrifolia Extract in Sterilizing B3 Infection Waste in the District Health Center of Enrekang Regency, guided by RAHMI AMIR and AMIR PATINTINGAN.The research is a quasi experimental design with the design of a post test only control group design . The purpose of this study was to determine the effectiveness of morinda citrifolia extract in sterilizing B3 infectious waste in Kabere Health Center in Enrekang Regency before and after the treatment of adding morinda citrifolia extract with various concentrations of 30%, 50%, and 70%, where three trials were conducted.Based on the results obtained in this study, there were germs on infectious waste (gauze) B3 before the treatment of the number of bacterial colonies in B3 infectious waste could not be counted (TBUD) and after the sterilization treatment the addition of morinda citrifolia extract occurred inhibition of bacterial colonies with three times trial. The treatment of the addition of 30% concentration of morinda citrifolia extract the average amount of inhibition of bacterial colonies obtained was 0.1 mm, at a concentration of 50% of morinda citrifolia extract the average amount of inhibition of bacterial colonies obtained was 0.08 mm, whereas at a concentration of 70% morinda citrifolia extract the average amount of inhibition of bacterial colonies obtained was 0.58 mm. The concentration dose of morinda citrifolia extract in this study was the more the amount of concentration added, the greater the inhibitory power given.Keywords: B3 Infection Waste, Morinda Citrifolia Extract

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