scholarly journals Skrining fitokimia dan uji toksisitas ekstrak daun balik angin (Mallotus Sp) terhadap larva Artemia salina Leach dengan metode brine shrimp lethality test (BSLT)

2017 ◽  
Vol 2 (2) ◽  
pp. 77
Author(s):  
Anderson Arnold Aloanis ◽  
Fahriana Fahriana ◽  
Haryadi Haryadi
Keyword(s):  
Acute Toxicity ◽  
Brine Shrimp ◽  
Artemia Salina ◽  
Control Group ◽  
Control Group Design ◽  
Brine Shrimp Lethality ◽  
Regular Treatment ◽  
Post Test ◽  
Young Leaves ◽  
Ethanol Extracts

One of the plants that are used to regular treatment is behind the wind (Mallotus sp.). Traditionally this is used for the relief of pain and increase stamina by way of boiling the seven pieces of young leaves and drink once a day in the morning. This research aims to know the acute toxicity of ethanol extracts of leaves behind the wind (Mallotus sp) against larvae of Artemia salina Leach BSLT method. The research method used is qualitative analysis of chemical compound and Brine Shrimp Lethality Test (BSLT) method with post-test-only control group design. The results of research on ethanol extracts of leaves behind the wind showed the value of LC50 of 32.061 ppm, so can be said of ethanol extracts of leaves behind the wind on this experiment of acute toxicity has on the larvae of Artemia salina Brine Shrimp method using Leach Lethality Test (BSLT).

scholarly journals Komposisi metabolit sekunder dan uji toksisitas fraksi etil asetat daun leban (Vitex pinnata Linn)

2021 ◽  
Vol 10 (1) ◽  
pp. 59
Author(s):  
Sabarudin - Sabarudin ◽  
Dewi Kurniasih
Keyword(s):  
Acute Toxicity ◽  
Brine Shrimp ◽  
Freeze Drying ◽  
Artemia Salina ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
X 24 ◽  
Test Control

ABSTRAK   Telah dilakukan skrining dan uji toksisitas tehadap ekstrak daun Leban (Vitex Pinnata Linn. Tujuan penelitian ini adalah untuk mengetahui komposisi kandungan senyawa metabolik sekunder dan potensi toksisitas akut ektrak daun muda. Penelitian ini adalah penelitian eksperimental dengan Only Post Test Control Group Design, ektraksi diawali dengan pengambilan sampel dari sekitar perkebunan penduduk di Desa Pudak. Pengolahan menjadi simplisia serbuk dengan melalui tahap sortasi basah, pencucian, pengeringan dan sortasi kering. Sediaan simplisia diekstraksi dengan cara masserasi direndam dalam pelarut n-heksana Merk selama 3 x 24 jam. Maserat atau filtrat selanjutnya dipisahkan menggunakan kaca corong pisah dan kertas saring, kemudian di uapkan dengan alat vacuum rotari evaporator sehingga diperoleh ekstrak mentah. Ektrak di fraksinasi berturut-turut menggunakan pelarut pertolium eter, etil asetat dan  metanol dan dikeringkan dengan metode pengeringan beku (freeze drying). Terhadap ekstrak dilakukan identifikasi untuk mengetahui kandungan senyawa metabolit sekunder. Selanjutnya setiap ektrak dilakukan uji toksisitas menggunakan metode Brine Shrimp Lethality Test (BSLT), dengan mengamati berapa jumlah larva udang (Artemia salina Leach) yang mati setelah 24 jam diberikan bahan uji. Hasil penelitian menunjukkan bahwa didalam ekstrak terdapat kandungan triterpena atau steroid, saponin dan flavonoid serta tidak ditemukan alkaloid. Uji toksisitas memberikan informasi bahwa hanya fraksi etil asetat menunjukkan ketoksikan akut. Analisis probit hasil pengamatan uji toksisitas memberikan hasil perhitungan dengan nilai  LC50 = 218 ppm, dengan kesimpulan daun vitex pinnata Linn memiliki toksisitas akut stergolong sedang.       ABSTRACT   Screening and toxicity tests have been carried out on Leban leaf extract (Vitex Pinnata Linn. The purpose of this study was to determine the composition of secondary metabolic compounds and the acute toxicity potential of young leaf extract. This study was an experimental study with Only Post Test Control Group Design, the extraction started with sampling from around the resident's plantations in Pudak Village Processing into powder simplicia by going through the stages of wet sorting, washing, drying and dry sorting Simplicia preparations are extracted by means of masseration soaked in n-hexane solvent Brand for 3 x 24 hours Maserate or filtrate is then separated using a separating funnel and filter paper, then evaporated using a vacuum rotary evaporator to obtain the crude extract.The extract was fractionated successively using pertolium ether, ethyl acetate and methanol as solvents and dried by freeze drying method. d identification to determine the content of secondary metabolites. Furthermore, each extract was tested for toxicity using the Brine Shrimp Lethality Test (BSLT) method, by observing how many shrimp larvae (Artemia salina Leach) died after 24 hours of being given the test material. The results showed that the extract contained triterpenes or steroids, saponins and flavonoids and no alkaloids were found. Toxicity test provided information that only the ethyl acetate fraction showed acute toxicity. Probit analysis of the results of the observation of the toxicity test gave the calculation results with a value of LC50 = 218 ppm, with the conclusion that the leaves of Vitex pinnata Linn have moderate acute toxicity.    

scholarly journals Uji Toksisitas Akut Ekstrak Spons Laut (Callyspongia aerizusa) terhadap Larva Artemia salina Leach dengan Metode Brine Shrimp Lethality Test

2017 ◽  
Vol 5 (2) ◽  
Author(s):  
Refrando M. Sondakh ◽  
Jimmy Posangi ◽  
Pemsi M. Wowor
Keyword(s):  
Acute Toxicity ◽  
Marine Sponge ◽  
Brine Shrimp ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Control Group ◽  
Control Group Design ◽  
Brine Shrimp Lethality ◽  
Toxicity Potential ◽  
Sponge Extract

Abstract: Indonesia has a great potential of marine biological resources. One of the coral reef ecosystems is marine sponge Callyspongia aerizusa that contains compounds of steroid, alkaloid, flavonoid, and terpenoid. In order to develop those substances as components of drugs, it is necessary to perform initial screening of its acute toxicity potential. This study was aimed to obtain the acute toxicity potential of marine sponge extract using Brine Shrimp Lethality Test (BSLT). This was an experimental study using the posttest only control group design. Samples were 180 larvae of Artemia salina Leach divided into 6 groups of 10 larvae. Each group was tested with three replications. The marine sponge extract final concentrations in the media which contained larvae consecutively in groups 1, 2, 3, 4, 5, and 6 were 1000 μg/ml, 500 μg/ml, 250 μg/ml, 100 μg/ml, 50 μg/ml, and 0 μg/ml as the negative control. The observation was focused to larvae that died 24 hours after the administration of the extract. LC 50 value of the marine sponge extract was analyzed by using probit analysis with SPSS 16.0. The results indicated that the extract final concentrations that killed the larvae were 1000 μg/ml, 500 μg/ml, and 250 μg/ml. The result of probit analysis indicated that LC 50 value of marine sponge extract was 992.468 μg/mL. Conclusion: The marine sponge extract had acute toxicity potential against Artemia salina Leach larvae according to BSLT with a LC 50 value <1000 μg/Ml.Keywords: Acute toxicity test, BSLT, LC 50, Callyspongia aerizusa marine sponge Abstrak: Indonesia memiliki potensi sumber daya alam hayati laut yang besar. Salah satu ekosistem terumbu karang ialah spons laut Callyspongia sp. yang mengandung senyawa steroid, alkaloid, flavonoid, dan terpenoid. Diperlukan skrining awal mengenai potensi toksisitas akut untuk pengembangan bahan baku obat. Penelitian ini bertujuan untuk mengetahui potensi toksisitas akut ekstrak spons Callyspongia sp. menggunakan Brine Shrimp Lethality Test (BSLT). Jenis penelitian ialah eksperimental dengan posttest-only control group design. Hewan uji ialah 180 ekor larva Artemia salina Leach yang dibagi dalam 6 kelompok, masing-masing terdiri dari 10 ekor. Tiap kelompok dilakukan pengulangan percobaan 3 kali. Ekstrak spons laut diberikan dalam media yang berisi larva. Konsentrasi akhir ekstrak dalam media yang berisi larva berturut-turut dalam kelompok 1, 2, 3, 4, 5, dan 6 ialah 1000 μg/ml, 500 μg/ml, 250 μg/ml, 100 μg/ml, 50 μg/ml dan 0 μg/ml sebagai kontrol negatif. Pengamatan dilakukan terhadap larva yang mati 24 jam setelah pemberian bahan uji. LC 50 ekstrak spons laut ditentukan dengan analisis probit menggunakan SPSS 16.0 for windows. Hasil penelitian menunjukkan beban konsentrasi ekstrak dalam media dapat membunuh larva secara berturut-turut dengan konsentrasi 1000 μg/ml, 500 μg/ml, dan 250 μg/ml. Hasil analisis probit menunjukkan nilai LC 50 dari ekstrak spons laut ialah 992,468 μg/mL. Simpulan: Ekstrak spons laut menunjukkan adanya potensi toksisitas akut terhadap larva Artemia salina Leach menggunakan BSLT dengan nilai LC50 <1000 μg/mL.Kata kunci: toksisitas, BSLT, spons laut Callyspongia aerizusa

scholarly journals UJI TOKSISITAS AKUT INFUSA BUAH MENGKUDU (Morinda citrifolia L.) PADA Artemia salina MENGGUNAKAN METODE BRINE SHRIMP LETHALITY TEST (BSLT)

2019 ◽  
Vol 2 (2) ◽  
pp. 75
Author(s):  
Nuri Mutammimah ◽  
Bilal S A Santoso
Keyword(s):  
Brine Shrimp ◽  
Artemia Salina ◽  
Negative Control ◽  
Test Method ◽  
Morinda Citrifolia ◽  
Control Group ◽  
Control Group Design ◽  
Brine Shrimp Lethality ◽  
Group Design ◽  
Post Test

ABSTRACT Mengkudu fruit (Morinda citifolia L.) is one of plants which has been known and used by indonesian society as traditional medicine. This plant contains alkaloid, phenol, steroid, and flavonoid which can cause toxicity. The aim of this study was to know about acute toxicity of mengkudu fruit infusions using brine shrimp lethality test method which will be indicated by LC50 value. This research was experimental research using post test only control group design that used negative control and 5 concentrations of infusion those are 1000 mg/L, 500 mg/L, 100 mg/L, 50 mg/L, and 10 mg/L. Death of larvae observed 24 hours after giving the infusiona. LC50 of mengkudu fruit infusion is 62,3 mg/L. The result of this study showed that mengkudu fruit infusions cause toxic effect. Keywords : Noni Fruit, Toxycity, Brine Shrimp Lethality Test, LC50   ABSTRAK Buah mengkudu (Morinda citrifolia L.) merupakan salah satu tanaman yang banyak dikenal dan digunakan oleh masyarakat sebagai obat tradisional. Tanaman ini mengandung senyawa alkaloid, fenol, steroid, dan flavonoid yang dapat menyebabkan tosiksitas dalam jumlah pemakaian tertentu. Penelitian ini bertujuan untuk mengetahui toksisitas akut infusa buah mengkudu dengan menggunakan metode Brine Shrimp Lethality Test yang akan ditunjukkan oleh nilai LC50. Penelitian ini termasuk penelitian ekperimental dengan post test only control group design. Uji ini menggunakan kontrol negatif dan 5 konsentrasi infusa yaitu 1000 mg/L, 500 mg/L, 100 mg/L, 50 mg/L, 10 mg/L. Kematian larva diamati 24 jam setelah pemberian infusa. Hasil penelitian menunjukkan nilai LC50 infusa buah mengkudu sebesar 62,3 mg/L. Kesimpulan dari penelitian ini adalah infus buah mengkudu bersifat toksik. Kata kunci: Toksisitas, Mengkudu, Brine Shrimp Lethality Test, LC50

scholarly journals Uji Toksisitas Akut Ekstrak dan Fraksi Kulit Batang Ketapang Laut (Terminalia Catappa L.) Menggunakan Metode BSLT

2020 ◽  
Vol 6 (1) ◽  
pp. 42
Author(s):  
Ari Sartinah ◽  
Yamin Yamin ◽  
Muhammad Arba ◽  
Nur Illiyyin Akib ◽  
Andi Nafisah Tendri Adjeng ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Ethyl Acetate ◽  
Program Analysis ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Artemia Salina ◽  
Test Method ◽  
Brine Shrimp Lethality ◽  
Terminalia Catappa ◽  
Water Fractions

AbstrakToksisitas akut merupakan kemampuan suatu bahan kimia (obat/bahan obat) dalam menimbulkan kerusakan  pada suatu organisme dalam waktu yang relatif singkat. Tujuan penelitian ini adalah untuk mengetahui potensi ketoksikan akut dari ekstrak metanol, fraksi etil asetat dan fraksi air kulit batang ketapang laut (Terminalia catappa L.). Pengujian toksisitas akut dilakukan dengan menggunakan metode BSLT (Brine Shrimp Lethality Test) dan menggunakan analisis program untuk mengetahui nilai LC50 dari ekstrak dan fraksi. Hasil uji toksisitas akut menunjukan nilai LC50  dari ekstrak metanol, fraksi etil asetat dan fraksi air kulit batang ketapang laut (Terminalia catappa L. )  berturut-turut 247,997 ppm; 400,666 ppm dan 618,046 ppm. Hal ini menunjukan bahwa ekstrak metanol batang ketapang laut (Terminalia catappa L.) bersifat paling toksik dibandingkan fraksi etil asetat dan fraksi air.Kata kunci: Ekstrak, Fraksi, Terminalia catappa L., BSLT, Artemia salina LeachAbstractAcute toxicity is the ability of chemical (drug/drug ingredient) to cause damage to organisms in a relatively short time. The purpose of this study was to determine the potential for acute toxicity of methanol extract, ethyl acetate and water fractions of sea ketapang stem skin (Terminalia catappa L.). Acute toxicity testing was performed using the BSLT (Brine Shrimp Lethality Test) method and program analysis to determine the LC50 values of extracts and fractions. The results of the acute toxicity test showed the LC50 values of methanol extract, ethyl acetate and water fractions of the plant stem skin consecutively were 247,997 ppm; 400,666 ppm and 618,046 ppm. This shows that the methanol extract of sea ketapang stems (Terminalia catappa L.) is the most toxic compared to the ethyl acetate and the water fraction.Keywords: Extract, Fraction, Terminalia catappa L., BSLT, Artemia salina Leach

scholarly journals ANALISIS SENYAWA METABOLIT SEKUNDER DAN UJI TOKSISITAS EKSTRAK ETANOL BATANG TANAMAN PATAH TULANG (Euphorbia tirucalli L.) DENGAN METODE Brine Shrimp Lethality Test (BSLT)

2014 ◽  
Vol 14 (2) ◽  
pp. 106 ◽  
Author(s):  
Grace S. Baud ◽  
Meiske S. Sangi ◽  
Harry S.J. Koleangan
Keyword(s):  
Secondary Metabolite ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Phytochemical Screening ◽  
Brine Shrimp Lethality ◽  
Euphorbia Tirucalli ◽  
Ethanol Extracts

ABSTRAK Telah dilakukan penelitian mengenai analisis senyawa metabolit sekunder dan uji toksisitas pada batang tanaman Patah tulang (Euphorbia tirucalli L.). Analisis senyawa metabolit sekunder dilakukan dengan skrining fitokimia yaitu senyawa alkaloid, flavonoid, triterpenoid, steroid, saponin dan tanin pada ekstrak segar dan kering batang tanaman Patah tulang. Penentuan toksisitas ekstrak etanol batang tanaman Patah tulang menggunakan metode Brine Shrimp Lethality Test (BSLT). Uji toksisitas digunakan hewan uji Artemia salina Leach sebagai bioindikator. Pada skrining fitokimia diperoleh positif flavonoid, tanin dan steroid sedangkan alkaloid, saponin dan triterpenoid negatif. Data pengujian toksisitas diperoleh dari analisis Lethality Concentration 50 (LC50) yang dilakukan dengan metode probit menggunakan perangkat lunak SPSS 20.0 (untuk sistem operasi Windows). Hasil uji toksisitas ekstrak etanol batang Patah tulang menunjukkan bahwa ekstrak segar maupun kering bersifat sitotoksik dengan nilai LC50 (7,994ppm) untuk ekstrak etanol batang segar  dan LC50 (9,940ppm) untuk ekstrak etanol batang kering. Kata kunci : Euphorbia tirucalli L., Senyawa metabolit sekunder, Toksisitas, Artemia salina Leach, LC50.   ANALYSIS OF SECONDARY METABOLITE COMPOUNDS AND TOXICITY TEST OF  STEM  PLANT ETHANOL EXTRACTS OF  PATAH TULANG (Euphorbia tirucalli L.) BY BRINE SHRIMP LETHALITY TEST’S METHOD (BSLT) ABSTRACT A research on the analysis of secondary metabolite compounds contained in the stem of  patah tulang (Euphorbia tirucalli L.) and its toxicity had been done. The analysis was performed through phytochemical screening of alkaloids, flavonoids, triterpenoids, steroids, saponins and tannins on extracts of fresh and dried stem of the plant. Toxicity of ethanol extract of the stem was determined using Brine Shrimp Lethality Test (BSLT) method with Artemia salina Leach as bioindicator. Phytochemical screening showed positive results for flavonoids, tannins, and steroids and negative one for alkaloids, saponins, and triterpenoids. Toxicity data were obtained from Lethality Concentration 50 (LC50) calculation trhrough probit analysis using SPSS 20.0 software (for Windows operating systems). Toxicity test showed that ethanol extracts of the fresh and dried stem were cytotoxic with LC50 value of 7.994 ppm and 9.940 ppm, respectively. Keywords: Euphorbia tirucalli L., Secondary metabolite compounds, Toxicity, Artemia salina Leach, LC50.

scholarly journals Pengaruh Pemberian Ekstrak Etanol Buah Masisin (Rhodomyrtus tomentosa Wight) terhadap Frekuensi, Konsistensi, dan Durasi Diare pada Hewan Coba Mencit Putih Jantan (Mus musculus) yang Diinduksi Oleum Ricini

2019 ◽  
Vol 5 (1) ◽  
pp. 22-40
Author(s):  
Angeline Novia Toemon ◽  
Austin Bertilova Carmelita ◽  
Astri Widiarti ◽  
Dian Mutiasari
Keyword(s):  
Mus Musculus ◽  
Positive Control ◽  
Negative Control ◽  
Study Data ◽  
Control Group ◽  
Control Group Design ◽  
Post Test ◽  
Ethanol Extracts ◽  
One Way Anova ◽  
Rhodomyrtus Tomentosa

People in Central Borneo which is often used to treat diarrhea is the fruit of masisin (Rhodomyrtus tomentosa Wight) with active compounds which are capable to treat diarrhea such as alkaloids, flavonoids, tannins, saponins, and steroids. Diarrhea is a defecate with a liquid or half-liquid feces, the content of water in a feces is more than usual, it is more than 200 g or 200 ml / 24 hours. To know the effectiveness of the extract of the fruit of masisin as an alternative to antidiarrheal treatment seen from the frequency, duration, and consistency of feces in male white mice (Mus musculus). This study design was a true experimental design with post-test control group design with 5 treatments for each group. This study started by making some extracts of Masisin fruit. Followed observation with 5 treatment groups animals. The induction of diarrhea was using oleum ricini, with aquadest as the negative control, and Loperamide HCL as the positive control. The dose of ethanol extracts of masisin fruit divided into 0.3 mg/g BW, 0.6 mg/g BW and 1.2 mg/g BW, all of the treatments were given orally. This study data was statistically analyzed with one way ANOVA. The results of one way ANOVA test showed that the value of p <0,05 meaning that there is the influence of EEBM on frequency, consistency and duration of diarrhea at doses 0.3 mg/g BW, 0.6 mg/g BW and 1.2 mg/Gbw. Based on observations ethanol extracts of masisin fruit had antidiarrheal effect at doses 0,3 mg/g BW, 0.6 mg/g BW and 1.2 mg/g BW in male white mice (Mus musculus).

scholarly journals A Cytotoxic Activity of Tahongai (Kleinhovia hospita Linn.) Leaves Extracts Using Brine Shrimp Lethality Test

2019 ◽  
Vol 4 (3) ◽  
pp. 60
Author(s):  
Indah Solihah ◽  
Herlina Herlina ◽  
Riana Sari Puspita Rasyid ◽  
Tri Suciati ◽  
Khairunnisa Khairunnisa
Keyword(s):  
Statistical Analysis ◽  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Brine Shrimp Lethality ◽  
Ethanol Extracts

Tahongai (Kleinhovia hospita Linn.) leaves had been known contain alkaloids, flavonoids, saponins, steroids, and tannins. At Komering, South Sumatera tribe, tahongai leaves had been known to treat tumor, cancer, polyps, acne, and dysmenorrhea. The study of cytotoxic activity of tahongai bark and stem were done. This study aims to determine the cytotoxic activity of tahongai leaves extracts using BSLT method. Tahongai leaves were extracted using gradual maceration with n-hexane, ethyl acetate, and ethanol 96%. Each extract was tested cytotoxic activity towards Artemia salina L. larvae. The yield of n-hexane, ethyl acetate, and ethanol extracts are 2,686%, 7,033%, and 7,933% respectively. Ethanol extract of tahongai leaves had the best cytotoxic activity with lethality value 76,667% at 500ppm. Statistical analysis with two way ANOVA showed extract and concentration had a significant (p<0,05) effect on larvae lethality percentage.

scholarly journals CURCUMINOID AND TOXICITY LEVELS OF ETHANOL EXTRACT OF JAVANESE GINGER (Curcuma xanthorriza) ON BRINE SHRIMP (Artemia salina) LARVAE AND ZEBRAFISH (Danio rerio) EMBRYOS

2017 ◽  
Vol 10 (4) ◽  
pp. 169 ◽  
Author(s):  
Gustini Syahbirin ◽  
Nurfadilawati Mumuh ◽  
Kusdiantoro Mohamad
Keyword(s):  
Acute Toxicity ◽  
Danio Rerio ◽  
Brine Shrimp ◽  
Zebrafish Embryo ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Toxicity Tests ◽  
Pericardial Edema ◽  
Curcuma Xanthorrhiza ◽  
Ethanol Extracts

Objective: This study was aimed at determining the levels of curcuminoids and analyzing the toxicity of ethanol extracts of Javanese ginger.Methods: Curcuminoid levels were determined using high-performance liquid chromatography, while the toxicity tests were done on larva of brine shrimp (Artemia salina) by using a brine shrimp lethality test (BSLT) method and embryos of zebrafish (Danio rerio) using a zebrafish embryo acute toxicity (ZFET) method.Results: The level curcuminoid of ethanol extracts was 10.5% dry wt., consisting of curcumin at the highest percentage (68.06%) followed bydesmethoxycurcumin (24.6%) and bisdemethoxycurcumin (1.41%). In BSLT method, the lethal concentration 50% values (LC) value of our ethanol extract was 238 ppm, whereas in ZFET method, the LC value at 96 hours after fertilization was 80 ppm. The ethanol extract of ginger caused major malformations of the pericardial edema of zebrafish embryos at a concentration of 100 ppm. 50Conclusion: The ethanol extract of Curcuma xanthorrhiza from Bogor contained curcuminoids consisting of curcumin, desmethoxycurcumin, and bisdemethoxycurcumin, with acute toxicity, caused major malformations on the pericardial edema in zebrafish embryos.Keywords: Curcuma xanthorrhiza, Curcuminoid, Toxicity, Zebrafish embryo acute toxicity.50

scholarly journals UJI TOKSISITAS AKUT INFUSA DAUN SIRSAK (Annona muricata) PADA LARVA Artemia salina MENGGUNAKAN METODE BRINE SHRIMP LETHALITY TEST

2020 ◽  
Vol 3 (1) ◽  
pp. 23
Author(s):  
Arinta Mayang ◽  
Bilal S A Santoso
Keyword(s):  
Acute Toxicity ◽  
Medicinal Plant ◽  
Brine Shrimp ◽  
Artemia Salina ◽  
Negative Control ◽  
Probit Analysis ◽  
Annona Muricata ◽  
Brine Shrimp Lethality ◽  
Lc50 Value ◽  
Chemical Contents

ABSTRACTAnnona muricata is a family of Annonaceae that has been known as a medicinal plant. Sirsakleaves contain alkaloids, tannins, and several other chemical contents including acetogenin.The aim of this study was to determine the acute toxicity of the infusion of Sirsak leaves onthe Artemia salina larvae by the Brine Shirmp Lethality Test (BSLT) method. This experimentalstudy used 5 treatment concentrations (10 mg/L, 50 mg/L, 100 mg/L, 500 mg/L, 1000mg/L) and 1 negative control. All treatments were repeated 3 times. The number of Artemiasalina larvae used for each concentration was 10 larvae. The number of dead larvae wascounted after 24 hours of treatment. Based on probit analysis, the LC50 value of Sirsak leafinfusion was 38,73 mg/L. The conclusion of this research is the infusion of Sirsak leaves istoxic.Keywords: Sirsak leaves, toxicity, BSLTABSTRAKSirsak (Annona muricata) adalah keluarga Annonaceae yang telah dikenal sebagai tanamanobat. Daun Sirsak mengandung alkaloid, tanin, dan beberapa kandungan kimia lainnya termasukacetogenin. Tujuan dari penelitian ini adalah untuk menentukan toksisitas akut dari infusdaun Sirsak pada larva Artemia salina dengan metode Brine Shirmp Lethality Test (BSLT).Studi eksperimental ini menggunakan 5 konsentrasi perlakuan (10 mg/L, 50 mg/L, 100 mg/L,500 mg/L, 1000 mg/L) dan 1 kontrol negatif. Semua perawatan diulang 3 kali. Jumlah larvaArtemia salina yang digunakan untuk setiap konsentrasi adalah 10 larva. Jumlah larva matidihitung setelah 24 jam perlakuan. Berdasarkan analisis probit, nilai LC50 infus daun Sirsakadalah 38,73 mg/L. Kesimpulan penelitian ini adalah infus daun Sirsak bersifat toksik.Kata kunci: daun sirsak, toksisitas, BSLT

scholarly journals Unripe Papaya Seed Ethanol Extract (Carica papaya, Linn.) Inhibits FSH and LH of Male Mice (Mus musculus)

2018 ◽  
Vol 11 (2) ◽  
pp. 979-984
Author(s):  
Bagus Komang Satriyasa ◽  
Agung Nova Mahendra ◽  
I. Gusti Kamasan Arijana ◽  
Dewa Made Ruspawan
Keyword(s):  
Family Planning ◽  
Ethanol Extract ◽  
Control Group ◽  
Male Mice ◽  
Control Group Design ◽  
Post Test ◽  
Local Plant ◽  
Ethanol Extracts ◽  
Wide Family ◽  
Papaya Seed

Family planning is a program designed to control the rate of population growth extensively in the country of Indonesia. In order to avoid of potential destructive effects of population explosion, a nation-wide Family Planning Program has been adopted long-standing in Indonesia. As to make it successful, family planning would have to involve with participation of both males and females. It was aimed to prove that the ethanol extracts of a local plant, Bali papaya, have antifertility function via inhibiting the secretion of FSH and LH. Randomized post-test only control group design was selected for this study. 38 male mice (strain Balb-C) were used and randomly divided into two equal groups. Only 0.5 ml double-distilled water was applicated orally for 36 days to controls; and 0.5 ml ethanol extract of unripe papaya seed with dose of 20 mg/20gr BW/day for 36 days to treatments. At the end of the experimental duration, blood samples of each individuals of both of the groups were collected for FSH and LH measurement. FSH and LH levels in controls were recorded as 3.379 and 15.718 mIU/ml, respectively. In experimental animals, the level of FSH was declined to 2.053 mIU/ml, while LH was measured as 8.626 mIU/ml. The reduction of both of the hormone was probably related to the active substances of extract. Administration of the ethanol extract of papaya seed reduces the average level of FSH and LH (p<0.05), significantly.

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