Ethanol Extract Of Centella Asiatica (L.) Urban Leaves Effectively Inhibit Streptococcus pyogenes and Pseudomonas aeruginosa by Invitro Test

2020 ◽  
Vol 2 (2) ◽  
pp. 69-76
Author(s):  
Dini Aulia Azmi ◽  
Nurlailah Nurlailah ◽  
Ratih Dewi Dwiyanti
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Streptococcus Pyogenes ◽  
Bacterial Infections ◽  
Herbal Medicines ◽  
Centella Asiatica ◽  
Ethanol Extract ◽  
Dilution Method ◽  
Initial Stage ◽  
Independent Variable

Streptococcus pyogenes and Pseudomonas aeruginosa are some of the causes of infectious diseases. Centella asiatica (L.) Urban has many benefits for humans, including overcoming fever, anti-bacterial, and anti-inflammatory. This study aims to determine the inhibition of Centella asiatica (L.) Urban leaves ethanol extract on the growth of Streptococcus pyogenes and Pseudomonas aeruginosa. This research is the initial stage of the development of herbal medicines to treat Streptococcus pyogenes and Pseudomonas aeruginosa infections. The independent variable was the concentration of ethanol extract of Centella asiatica (L.) Urban leaves and the dependent variable was the growth of Streptococcus pyogenes and Pseudomonas aeruginosa. The anti-bacterial activity test was carried out by the liquid dilution method. The concentrations used are 20%, 40%, 60%, 80%. 100% The results showed that the minimum inhibitory concentration (MIC) against Streptococcus pyogenes: 40% and Pseudomonas aeruginosa: 40%. Minimum bactericidal concentration (MBC) results for Streptococcus pyogenes: 60% and Pseudomonas aeruginosa: 60%. So it can be concluded that there is inhibition of the ethanol extract of Centella asiatica (L.) Urban leaves on the growth of Streptococcus pyogenes and Pseudomonas aeruginosa. Centella Asiatica (L.) Urban extract has potential as herbal medicine against bacterial infections but requires further research to determine its effect in vivo.

Garlic Extract (Allium sativum L.) Effectively Inhibits Staphylococcus aureus and Escherichia coli by Invitro Test

2020 ◽  
Vol 2 (2) ◽  
pp. 61-68
Author(s):  
Agnina Listya Anggraini ◽  
Ratih Dewi Dwiyanti ◽  
Anny Thuraidah
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacterial Infections ◽  
Allium Sativum ◽  
Antibacterial Properties ◽  
Herbal Medicines ◽  
Garlic Extract ◽  
Dilution Method ◽  
Initial Stage

Infection is a disease caused by the presence of pathogenic microbes, including Staphylococcus aureus and Escherichia coli. Garlic (Allium sativum L.) has chemical contents such as allicin, alkaloids, flavonoids, saponins, tannins, and steroids, which can function as an antibacterial against Staphylococcus aureus and Escherichia coli. This study aims to determine the antibacterial properties of garlic extract powder against Staphylococcus aureus and Escherichia coli. This research is the initial stage of the development of herbal medicines to treat Staphylococcus aureus and Escherichia coli infections. The antibacterial activity test was carried out by the liquid dilution method. The concentrations used were 30 mg/mL, 40 mg/mL, 50 mg/mL, 60 mg/mL and 70 mg/mL. The results showed that the Minimum Inhibitory Concentration (MIC) against Staphylococcus aureus and Escherichia coli was 40 mg/mL and 50 mg / mL. Minimum Bactericidal Concentration (MBC) results for Staphylococcus aureus and Escherichia coli are 50 mg/mL and 70 mg/mL. Based on the Simple Linear Regression test, the R2 value of Staphylococcus aureus and Escherichia coli is 0.545 and 0.785, so it can be concluded that there is an effect of garlic extract powder on the growth of Staphylococcus aureus and Escherichia coli by 54.5% and 78.5%. Garlic (Allium sativum L.) extract powder has potential as herbal medicine against bacterial infections but requires further research to determine its effect in vivo.

scholarly journals STANDARDISASI EKSTRAK PEGAGAN, CENTELLA ASIATICA SEBAGAI OBAT HERBAL TERSTANDAR HEPATOPROTEKTOR

2015 ◽  
Vol 17 (2) ◽  
pp. 185-193
Author(s):  
Puspa Dewi N Lotulung ◽  
Sri Handayani ◽  
Teni Ernawati ◽  
Tri Yuliani ◽  
Nina Artanti ◽  
...  
Keyword(s):  
Body Weight ◽  
Microbial Contamination ◽  
Raw Materials ◽  
Herbal Medicines ◽  
Centella Asiatica ◽  
Ethanol Extract ◽  
Ash Content

Herbal medicinal products would be affected by the quality of raw materials. In turn, the quality of raw material will also be influenced by various factors such as soil conditions, cultivation, post-harvest processing, and the processing of raw materials into crude drug or extract. Therefore, in order to make good herbal medicines, it is necessary to make standardization of herbal extracts that produced herbal medicines that have the same quality and functions of effectiveness in each process. From preliminary studies that have been done, Centella asiatica is one of the potential plants as a source of hepatoprotective compounds. Test in vivo and in vitro against Centella asiatica extracts have shown very good results. Ethyl acetate extract with 17.5 mg/kg of doses body weight and butanol 228.8 mg/kgof doses body weight has been applied for in vivo test using mice induced by CCl4; theydemonstrated hepatoprotective effects. Ethyl acetate extracts were able to reduce levels of the enzyme alanine aminotransferase (ALT) and aspartate aminotransferase (AST) by 56 % and 44 % respectively while butanol extract can reduce the enzymes AST levels by 3%. Standardizationof Centella asiatica extract performed in this study was the characterization of the extract in the form of non-specific and specific parameters corresponding to the reference of PPOMN (Ministry of health Republic of Indonesia, 2000) such as levels of drying shrinkage, ash content, total plate count microbial contamination, levels of water-soluble compounds, levels of compounds that are soluble in ethanol, phytochemical test, total phenolic content, total flavonoid content and the determination of Pb and Cd weight.The results showed that non-specific parameters for the ethanol extract of Centella asiatica were requirements based on Herbal Pharmacopoeia in 2008 which includes parameters such as determination of shrinkage on drying ≤ 10%, ash content ≤ 16.6% and negative microbial contamination. Specific parameters for the ethanol extract of Centella asiatica have met the requirements of Herbal pharmacopeia in 2008.Keywords: Centellaasiatica, hepatoprotective, standardized herbal medicine, specificparameters, and non-specific parameters

scholarly journals EFEKTIVITAS EKSTRAK ETANOL DAUN KIRINYUH (CHROMOLAENA ODORATA L) SEBAGAI ANTIBAKTERI SALMONELLA TYPHI DAN STAPHYLOCOCCUS AUREUS

2020 ◽  
Vol 2 (3) ◽  
pp. 158-168
Author(s):  
Fadia Fadia ◽  
Nurlailah Nurlailah ◽  
Tini Elyn Helmiah ◽  
Leka Lutpiatina
Keyword(s):  
Staphylococcus Aureus ◽  
Bacterial Infections ◽  
Pathogenic Bacteria ◽  
Herbal Medicines ◽  
Ethanol Extract ◽  
Salmonella Typhi ◽  
Antibacterial Drug ◽  
Chromolaena Odorata ◽  
And Staphylococcus Aureus

        Salmonella typhi and Staphylococcus aureus are pathogenic bacteria that cause infectious diseases. Treatment of these diseases can be done by giving antibacterial drugs. One of the plants that can be used as an antibacterial drug is the leaves of Chromolaena odorata L. due to the presence of chemical compounds such as flavonoids, tannins, and saponins that have the potential as antibacterial. This study aims to determine the Minimum Inhibitory Concentration (MIC), and Minimum Bactericidal Concentration (MBC) ethanol extract of Chromolaena odorata L. leaves on the growth of Salmonella typhi and Staphylococcus aureus. This research is the initial stage of the development of herbal medicines to treat Salmonella typhi and Staphylococcus aureus infections. The study consisted of 5 treatments, namely concentrations of 20%, 40%, 60%, 80%, and 100% ethanol extract of Chromolaena odorata L. leaves with five repetitions. The antibacterial activity test was carried out by MIC and MBC methods. The research material uses leaves from Chromolaena odorata L. in the Sungai Besar area, Banjarbaru, South Kalimantan, Indonesia. The results showed that the average MIC yield of ethanol extract of Chromolaena odorata L. leaves against Salmonella typhi: 20% and Staphylococcus aureus: 20%. While the average yield of MBC values ​​against Salmonella typhi: 40% and Staphylococcus aureus: 40%. Based on the results obtained, it can be concluded that the ethanol extract of Chromolaena odorata L. leaves has an inhibitory effect on the growth of Salmonella typhi and Staphylococcus aureus. Chromolaena odorata L. leaves has potential as herbal medicine against bacterial infections but requires further research to determine its effect in vivo.

scholarly journals INHIBITORY ACTIVITY OF ALLIUM SATIVUM L. EXTRACT AGAINST STREPTOCOCCUS PYOGENES AND PSEUDOMONAS AERUGINOSA

2019 ◽  
Vol 3 (2) ◽  
pp. 72
Author(s):  
Natasha Hana Savitri ◽  
Danti Nur Indiastuti ◽  
Manik Retno Wahyunitasari
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Streptococcus Pyogenes ◽  
Bacterial Infections ◽  
Allium Sativum ◽  
Antibacterial Effect ◽  
Antibacterial Properties ◽  
Antibiotic Activity ◽  
Garlic Extract ◽  
Dilution Method ◽  
Before And After

Background: One of the most common health problems is infectious diseases. Infectious disease can be caused by bacteria. There were two groups of bacteria based on the staining, Gram-positive and Gram-negative bacteria. Purpose: Antibiotics are the main therapy used in the incidence of bacterial infections. But over time, some antibiotics became resistance. Several studies have shown that garlic has an antibacterial effect. The content of allicin, ajoene, saponins, and flavonoids is found in garlic which has antibacterial properties. The antibiotic activity test of garlic was carried out on the bacteria Streptococcus pyogenes and Pseudomonas aeruginosa. The goal of this study is to investigate the antibacterial effect of Allium sativum L. extract against Streptococcus pyogenes and Pseudomonas aeruginosa. Methods: Garlic extract was made using the maceration method using 96% alcohol as the solvent. Tube dilution method elected to observe garlic antibiotic activity. This test aims to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). There were eight different concentration used, i.e. 2 grams/ml, 1 gram/ml, 0.5 gram/ml, 0.25 gram/ml, 0.125 gram/ml, 0.0625 gram/ml, 0.03125 gram/ml, and 0.015625 gram/ml. Replication is done three times. Results: In this experiment, the extract produced was turbid that MIC could not be determined and there was no momentous differentiation between before and after treatment. There was no growth of Streptococcus pyogenes in 1 gram/ml and Pseudomonas aeruginosa in 0.5 gram/ml. This number indicates the MBC for each bacteria. Conclusion: Garlic (Allium sativum L.) has an effect of bactericidal activity, it can perform as an antibacterial for Streptococcus pyogenes and Pseudomonas aeruginosa. Garlic extract was more effective for Pseudomonas aeruginosa than Streptococcus pyogenes.

scholarly journals Tackling Virulence of Pseudomonas aeruginosa by the Natural Furanone Sotolon

Antibiotics ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 871
Author(s):  
Mohammed F. Aldawsari ◽  
El-Sayed Khafagy ◽  
Ahmed Al Saqr ◽  
Ahmed Alalaiwe ◽  
Hisham A. Abbas ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Virulence Factors ◽  
Bacterial Infections ◽  
Therapeutic Agent ◽  
Bacterial Resistance ◽  
Inhibitory Concentration ◽  
Bacterial Biofilm ◽  
Resistant Bacteria ◽  
Resistance Development

The bacterial resistance development due to the incessant administration of antibiotics has led to difficulty in their treatment. Natural adjuvant compounds can be co-administered to hinder the pathogenesis of resistant bacteria. Sotolon is the prevailing aromatic compound that gives fenugreek its typical smell. In the current work, the anti-virulence activities of sotolon on Pseudomonas aeruginosa have been evaluated. P. aeruginosa has been treated with sotolon at sub-minimum inhibitory concentration (MIC), and production of biofilm and other virulence factors were assessed. Moreover, the anti-quorum sensing (QS) activity of sotolon was in-silico evaluated by evaluating the affinity of sotolon to bind to QS receptors, and the expression of QS genes was measured in the presence of sotolon sub-MIC. Furthermore, the sotolon in-vivo capability to protect mice against P. aeruginosa was assessed. Significantly, sotolon decreased the production of bacterial biofilm and virulence factors, the expression of QS genes, and protected mice from P. aeruginosa. Conclusively, the plant natural substance sotolon attenuated the pathogenicity of P. aeruginosa, locating it as a plausible potential therapeutic agent for the treatment of its infections. Sotolon can be used in the treatment of bacterial infections as an alternative or adjuvant to antibiotics to combat their high resistance to antibiotics.

scholarly journals Analysis of Susceptibility Patterns of Pseudomonas aeruginosa and Isolation, Characterization of Lytic Bacteriophages Targeting Multi Drug Resistant Pseudomonas aeruginosa

2018 ◽  
Vol 11 (2) ◽  
pp. 1105-1117 ◽  
Author(s):  
Shri Natrajan Arumugam ◽  
Akarsh Chickamagalur Rudraradhya ◽  
Sathish Sadagopan ◽  
Sunilkumar Sukumaran ◽  
Ganesh Sambasivam ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Host Range ◽  
Polymyxin B ◽  
Multidrug Resistant ◽  
Clinical Isolates ◽  
Dilution Method ◽  
Drug Resistant ◽  
Susceptibility Pattern ◽  
Infectivity Rate

Pseudomonas aeruginosa is known to be a major cause of Hospital Acquired Infections leading to high mortality in immune-compromised patients. Due to precipitous rise in antibiotic resistance, bacteriophages are significant alternative therapeutic approach for treatment and to combat resistance development. Objective of the current study was to identify MDR Pseudomonas aeruginosa from clinical isolates and to isolate bacteriophages from sewage samples against these MDR Pseudomonas aeruginosa strains. One hundred and forty-four Pseudomonas isolates were tested for their susceptibility pattern with 13 different antibiotics by micro-broth dilution method. Frequency of multidrug resistant (MDR) and Extensive Drug resistant (XDR) of Pseudomonas aeruginosa were found to be 35.5% and 23.6%, respectively. 7.61% isolates were identified as Pan drug resistant (PDR). Rate of susceptibility pattern were Piperacillin/Tazobactam 75%, Polymyxin B 74.6%, Meropenem 73.6%, Colistin 69.2%, Cefepime 54.9%, Ciprofloxacin 54.2%, Gentamicin 54.2%, Aztreonam 53.5%, Tobramycin 47.9%, Ticarcillin/Clavulanic acid 46.9%, Ertapenem 45.8%, Ceftazidime 40.3% and Imipenem 39.2%. Ninety-four bacteriophages were isolated from sewage samples against Pseudomonas aeruginosa PAO1/ATCC9027/clinical strains and host range testing study was carried out with all MDR clinical isolates. Among 51 MDR strains 34 strains were infected by phages. Phage infectivity rate were calculated for individual phages based on their host range infectivity results. AP025 and AP006 phages exhibited good infectivity rate of 39% and 30% respectively against MDR strains. Combination of 5 phages (AP002, AP006, AP011, AP025 and AP067) lysed 62.7% of the strains. Based on the obtained results, phages could be employed for treatment of infections caused by MDR strains with substantiated in-vivo experiments.

scholarly journals Tasked with a Challenging Objective: Why Do Neutrophils Fail to Battle Pseudomonas aeruginosa Biofilms

Pathogens ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 283 ◽  
Author(s):  
Jennifer Geddes-McAlister ◽  
Abirami Kugadas ◽  
Mihaela Gadjeva
Keyword(s):  
United States ◽  
Pseudomonas Aeruginosa ◽  
Bacterial Infections ◽  
The United States ◽  
Multidrug Resistant ◽  
Bacterial Biofilms ◽  
Spectroscopy Analysis ◽  
Mass Spectroscopy Analysis ◽  
Novel Therapeutic

Multidrug-resistant (MDR) bacterial infections are a leading cause of mortality, affecting approximately 250,000 people in Canada and over 2 million people in the United States, annually. The lack of efficacy of antibiotic-based treatments is often caused by inability of the drug to penetrate bacterial biofilms in sufficient concentrations, posing a major therapeutic challenge. Here, we review the most recent information about the architecture of Pseudomonas aeruginosa biofilms in vivo and describe how advances in imaging and mass spectroscopy analysis bring about novel therapeutic options and challenge existing dogmas.

scholarly journals Methionine Limitation Impairs Pathogen Expansion and Biofilm Formation Capacity

2019 ◽  
Vol 85 (9) ◽  
Author(s):  
A. Jochim ◽  
T. Shi ◽  
D. Belikova ◽  
S. Schwarz ◽  
A. Peschel ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Human Serum ◽  
Bacterial Infections ◽  
Bacterial Species ◽  
Biosynthesis Pathway ◽  
Methionine Biosynthesis ◽  
Content Type

ABSTRACTMultidrug-resistant bacterial pathogens are becoming increasingly prevalent, and novel strategies to treat bacterial infections caused by these organisms are desperately needed. Bacterial central metabolism is crucial for catabolic processes and provides precursors for anabolic pathways, such as the biosynthesis of essential biomolecules like amino acids or vitamins. However, most essential pathways are not regarded as good targets for antibiotic therapy since their products might be acquired from the environment. This issue raises doubts about the essentiality of such targets during infection. A putative target in bacterial anabolism is the methionine biosynthesis pathway. In contrast to humans, almost all bacteria carry methionine biosynthesis pathways which have often been suggested as putative targets for novel anti-infectives. While the growth of methionine auxotrophic strains can be stimulated by exogenous methionine, the extracellular concentrations required by most bacterial species are unknown. Furthermore, several phenotypic characteristics of methionine auxotrophs are only partly reversed by exogenous methionine. We investigated methionine auxotrophic mutants ofStaphylococcus aureus,Pseudomonas aeruginosa, andEscherichia coli(all differing in methionine biosynthesis enzymes) and found that each needed concentrations of exogenous methionine far exceeding that reported for human serum (∼30 µM). Accordingly, these methionine auxotrophs showed a reduced ability to proliferate in human serum. Additionally,S. aureusandP. aeruginosamethionine auxotrophs were significantly impaired in their ability to form and maintain biofilms. Altogether, our data show intrinsic defects of methionine auxotrophs. This result suggests that the pathway should be considered for further studies validating the therapeutic potential of inhibitors.IMPORTANCENew antibiotics that attack novel targets are needed to circumvent widespread resistance to conventional drugs. Bacterial anabolic pathways, such as the enzymes for biosynthesis of the essential amino acid methionine, have been proposed as potential targets. However, the eligibility of enzymes in these pathways as drug targets is unclear because metabolites might be acquired from the environment to overcome inhibition. We investigated the nutritional needs of methionine auxotrophs of the pathogensStaphylococcus aureus,Pseudomonas aeruginosa, andEscherichia coli. We found that each auxotrophic strain retained a growth disadvantage at methionine concentrations mimicking those availablein vivoand showed that biofilm biomass was strongly influenced by endogenous methionine biosynthesis. Our experiments suggest that inhibition of the methionine biosynthesis pathway has deleterious effects even in the presence of external methionine. Therefore, additional efforts to validate the effects of methionine biosynthesis inhibitorsin vivoare warranted.

scholarly journals Novel Lytic Phages Protect Cells and Mice against Pseudomonas aeruginosa Infection

2021 ◽  
Author(s):  
Feng Chen ◽  
Xingjun Cheng ◽  
Jianbo Li ◽  
Xiefang Yuan ◽  
Xiuhua Huang ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Mouse Models ◽  
Bacterial Infections ◽  
Phage Therapy ◽  
Inflammatory Responses ◽  
Multi Drug Resistance ◽  
Bacterial Strains ◽  
Genetic Traits

With the fast emergence of serious antibiotic resistance and the lagged discovery of novel antibacterial drugs, phage therapy for pathogenic bacterial infections has acquired great attention in the clinics. However, development of therapeutic phages also faces tough challenges, such as laborious screening and time to generate effective phage drugs since each phage may only lyse a narrow scope of bacterial strains. Identifying highly effective phages with broad host ranges is crucial for improving phage therapy. Here, we isolated and characterized several lytic phages from various environments specific for Pseudomonas aeruginosa by testing their growth, invasion, host ranges, and potential for killing targeted bacteria. Importantly, we identified several therapeutic phages (HX1, PPY9, and TH15) with broad host ranges to lyse laboratory strains and clinical isolates of P. aeruginosa with multi-drug resistance (MDR) both in vitro and in mouse models. In addition, we analyzed critical genetic traits related to the high-level broad host coverages by genome sequencing and subsequent computational analysis against known phages. Collectively, our findings establish that these novel phages may have potential for further development as therapeutic options for patients who fail to respond to conventional treatments. IMPORTANCE Novel lytic phages isolated from various environmental settings were systematically characterized for their critical genetic traits, morphology structures, host ranges against laboratory strains and clinical multi-drug resistant (MDR) Pseudomonas aeruginosa, and antibacterial capacity both in vitro and in mouse models. First, we characterized the genetic traits and compared with other existing phages. Furthermore, we utilized acute pneumonia induced by laboratorial strain PAO1, and W19, an MDR clinical isolate and chronic pneumonia by agar beads laden with FDR1, a mucoid phenotype strain isolated from the sputum of a cystic fibrosis (CF) patient. Consequently, we found that these phages not only suppress bacteria in vitro but also significantly reduce the infection symptom and disease progression in vivo, including lowered bug burdens, inflammatory responses and lung injury in mice, suggesting that they may be further developed as therapeutic agents against MDR P. aeruginosa.

scholarly journals A study of antimicrobial activity of polyphenols derived from wood

2018 ◽  
pp. 46-49 ◽  
Author(s):  
A. B. Shevelev ◽  
E. P. Isakova ◽  
E. V. Trubnikova ◽  
N. La Porta ◽  
S. Martens ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Drug Resistance ◽  
Bacterial Infections ◽  
In Vivo Studies ◽  
Radial Diffusion Assay ◽  
Diffusion Assay ◽  
Novel Antibiotics

Due to the spreading and increasing drug resistance of pathogens, the search for novel antibiotics is becoming ever more important. Plant-derived polyphenols are a vast and promising class of compounds with a potential to fight infectious diseases. Still, they are not routinely used in clinical practice. No reports on the in vivo studies of these compounds have been presented. The aim of our work was to compare the antimicrobial activity of resveratrol (stilbene), dihydroquercetin and dihydromyricetin (flavonols) extracted from the bark and wood of conifers against the dermatophytes Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans. Using the radial diffusion assay, we established that dihydroquercetin, resveratrol and dihydromyricetin exhibit high activity against S. aureus even at the smallest possible concentrations of 0.22, 0.15, and 0.15 mM, respectively. In contrast, the highest achievable concentrations of these compounds in the solutions (21.5, 15.5 and 15.0 mM for dihydroquercetin, resveratrol and dihydromyricetin, respectively) have no effect on the growth of P. aeruginosa and C. albicans. These findings suggest that polyphenols derived from conifers could have a potential to be used as a medicine for topical application to treat bacterial infections of the skin caused by S. aureus.

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