scholarly journals FUNCTIONAL GROUPS OF BIFIDOFLORA OF INTESTINAL MICROBIOTA IN ASSOCIATIVE SYMBIOSIS OF HUMAN

2018 ◽  
pp. 3-9 ◽  
Author(s):  
O. V. Bukharin ◽  
E. V. Ivanova ◽  
N. B. Perunova ◽  
I. A. Nikiforov
Keyword(s):  
Functional Groups ◽  
Experimental Studies ◽  
Statistical Processing ◽  
Antagonistic Activity ◽  
Immunological Methods ◽  
Human Gut ◽  
The Third ◽  
Cytokine Balance ◽  
Human Large Intestine ◽  
Associative Symbiosis

Aim. Aim of the research is the identification of functional groups of human gut bifidoflora based on analysis of the spectrum of metabolites features, proteome, bioprofile, immunoregulatory properties and the ability to differentiate «self/non-self» among the associative microbiota. Materials and methods. The materials are 260 strains ofbifidobacteria isolated from 122 intestinal microsymbiocenoses. Experimental studies were carried out using bacteriological, chromatographic and immunological methods. Statistical processing of material is carried out by means of the package Statistica 10.0 using of k-cluster analysis and discriminant method. Results. As a result ofthe work, 3 clusters containing strains of various types of bifidobacteria were identified. The first cluster was represented by B. bifidum and was characterized by the antipeptide activity of the strains with respect to FNO-a and INF-y, IL-10. In the second cluster of the B. longum culture predominated, where the parameters of the backbone factor of microsymbiocenosis, the ability to microbial recognition, antagonistic activity and production of acetic acid were significant. In the third cluster the species composition of bifidobacteria was diverse and products of butyric, caproic acids and their isoforms were the informative tests. Conclusion. The key function of bifido-flora in the regulation of the homeostasis of the intestinal biotope is realized by the formation of functional clusters, among which the first group participates in the formation of the cytokine balance, the second group is responsible for the discrimination of associative microbiota and direct protection of the biotope from pathogens, and the third is necessary to maintain the barrier metabolic function of enterocytes in the human large intestine.

scholarly journals Influence of sowing time on the yield of different varieties of soybeans

2021 ◽  
Vol 937 (2) ◽  
pp. 022127
Author(s):  
L M Kelekhsashvili
Keyword(s):  
Experimental Studies ◽  
Statistical Processing ◽  
Mineral Fertilizers ◽  
North Caucasus ◽  
Sowing Time ◽  
Scientific Center ◽  
Average Value ◽  
The Third ◽  
The North ◽  
Sowing Period

Abstract Experimental studies were carried out on the experimental fields of the North Caucasus Research Institute of Mining and Piedmont Agriculture of the Vladikavkaz Scientific Center of the Russian Academy of Sciences. Trial establishment, phenological observations, and statistical processing of the obtained results were carried out using generally accepted methods. It was found that the highest seed yields for the studied varieties (Vilana, Slavia, Chara) were obtained in the third sowing period. So, for the Vilana variety (on average for 2018-2020, background - without fertilizers), the yield in the first period (10°C) was 1.87 t/ha, in the second (12°C) - 1.91 t/ha, in the third (14°C) - 2.06 t/ha, and in the fourth (16°C) - 1.97 t/ha. Indicators for the Slavia and Chara varieties were slightly lower. When mineral fertilizers were applied at the rate of P120K60, the yield of the studied varieties increased in comparison with the background P60K30. So, for the Vilana variety (average value over 3 years), the yield was higher in the first sowing period by 0.22 t/ha, in the second - by 0.31 t/ha, in the third - by 0.57 t/ha, and in the fourth - by 0.34 t/ha. From the given data it can be seen that the highest yield was noted in the third sowing period. A similar pattern was also observed for the Slavia and Chara varieties. So, for the Slavia variety, the yield in the third sowing period was 2.74 t/ha, in the fourth - 2.64 t/ha, in the second - 2.56 t/ha, in the first - 2.35 t/ha, and for the Chara variety, respectively: 2.60; 2.48; 2.45, and 2.31 t/ha.

scholarly journals THEORETICAL RESEARCH OF THE EXTERNAL TEMPERATURE INFLUENCE ON THE TRACTION AND SPEED PROPERTIES AND THE FUEL ECONOMY OF CARGO-CARRYING VEHICLES

2020 ◽  
pp. 1-9
Author(s):  
Zhasur Kulmukhamedov ◽  
Ravshan Khikmatov ◽  
Alisher Saidumarov ◽  
Yulduz Kulmukhamedova
Keyword(s):  
Experimental Data ◽  
Fuel Economy ◽  
Experimental Studies ◽  
Statistical Processing ◽  
Research Data ◽  
Theoretical Research ◽  
Temperature Influence ◽  
External Temperature ◽  
Average Speed ◽  
The Mathematical Model

The manuscript proposes analytical methods for calculating fuel economy and traction-speed properties when modeling the movement of cargo-carrying vehicles on real routes, based on theoretical and experimental studies in a hot and dry climate, which allows for determining the efficiency of cargo-carrying vehicles objectively in terms of traction and speed, fuel and economic indicators. Using the statistical processing of experimental, theoretical research data, the authors calculate the coefficient X2, which allows for evaluating the adequacy of the mathematical model and experimental data. As an example, the manuscript provides for an assessment of fuel economy and traction and speed properties. The authors presented the results in graphs for the ease of evaluating the effect of external temperature on fuel consumption and the average speed of a road train. The authors’ methodology allows for determining the efficiency of cargo-carrying vehicles in a hot and dry climate.

scholarly journals Iron reduction from concentrates of hydrometallurgical dressing

2021 ◽  
Vol 64 (10) ◽  
pp. 728-735
Author(s):  
I. A. Rybenko ◽  
O. I. Nokhrina ◽  
I. D. Rozhikhina ◽  
M. A. Golodova ◽  
I. E. Khodosov
Keyword(s):  
Experimental Study ◽  
Thermal Decomposition ◽  
Thermodynamic Modeling ◽  
Iron Reduction ◽  
Solid Phase ◽  
Experimental Studies ◽  
Statistical Processing ◽  
Reducing Agent ◽  
Volatile Components ◽  
Software Complex

The article presents results of theoretical and experimental studies of the processes of iron solid-phase reduction from an iron-containing concentrate obtained as a result of hydrometallurgical dressing of ferromanganese and polymetallic manganese-containing ores with coals of grades D (long-flame) and 2B (brown). The method of thermodynamic modeling using TERRA software complex was used to study the reducing properties of hydrocarbons by calculating equilibrium compositions in the temperature range of 373 - 1873 K. The authors obtained the dependences of compositions and volume of the gas phase formed as a result of the release of volatile components during heating on the temperature for the coals of the grades under consideration. As a result of thermodynamic modeling, the optimal temperatures and consumption are determined, which ensure the complete iron reduction from an iron-containing concentrate. The results of experimental studies were obtained by modern research methods using laboratory and analytical equipment, as well as methods of statistical processing. Results of the coals analysis carried out using the Setaram LabSys Evo thermal analyzer showed that the process of thermal decomposition of coals of the studied grades proceeds according to general laws. The process of thermal decomposition of long-flame coal proceeds less intensively than of brown coal. The results of an experimental study of the processes of thermal decomposition of reducing agents have shown that volumes of the gas phases, formed when coals are heated to a temperature of 1173 K in an argon atmosphere, practically coincide with the calculated values. As a result of thermodynamic modeling and experimental study, the optimal consumption of D and 2B grades of coal is determined at a temperature of 1473 K. The best reducing agent with a minimum specific consumption is long-flame coal of D grade. When determining the optimal amount of reducing agent in charge mixtures during the study of metallization processes, it was found that with an excess of reducing agent, it is possible to achieve almost complete extraction (98 - 99 %) of iron from the concentrate.

scholarly journals Metabolic and molecular-genetic changes in the liver during carbon tetrachloride intoxication

2020 ◽  
Vol 99 (9) ◽  
pp. 996-1000
Author(s):  
Denis O. Karimov ◽  
Tatyana G. Kutlina ◽  
Guzel’ F. Mukhammadiyeva ◽  
Yana V. Valova ◽  
Samat S. Baygildin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Blood Serum ◽  
Pearson Correlation ◽  
Molecular Genetic ◽  
Experimental Studies ◽  
Pcr Amplification ◽  
Statistical Processing ◽  
Male Rats ◽  
Clinical Test ◽  
Genetic Changes

Introduction. Toxic hepatitis (TH) is a complex and multifaceted disease, the development of which is mediated by a complex of biochemical and molecular genetic interactions. The current understanding of the pathogenesis of TH and, as a consequence, its treatment is based on standardization of the phenotype of the disease, often without taking into account metabolic disorders within the cells. Material and methods. experimental studies were performed on white outbred male rats weighing 200-220 g. A 50% solution of TCM was used as a toxicant. Biochemical studies were performed on a laboratory medical photometer “Stat Fax 3300” using clinical test kits and control materials manufactured by Vector-Best LLC. Liver tissue for histological examination was subjected to the standard histological procedure and paraffin embedding. Sections 5-7 μm thick were stained with hematoxylin-eosin. Gene expression analysis was performed using real-time PCR amplification on a RotorGene instrument (QIAGEN). Statistical processing of experimental data was performed using the Pearson correlation coefficient and one-way analysis of variance (ANOVA). The results were considered reliable at p <0.05. Results. As a result of the analysis of the correlation of the expression of the studied genes and the level of biochemical parameters, it was found that the correlation of the expression of the Nfe2l2 and Gstm1 genes was r = 0.812 (p = 0.0001). The dynamics of gene expression of Chek, Gstm1, Gstp1, Nfe2l2, had a negative correlation with the level of AST activity in blood serum. And the expression of the genes Chek, Gclc, Gstm1, Nfe2l2, Ripk, Sod1 with an index of ALT activity in the blood serum. After 72 hours, the expression of almost all of the studied genes became multidirectional. And the correlation between indices is often not determined. An analysis of the relationship between the level of cytolysis enzymes and the correlation level of the studied genes showed that after 72 hours the correlation was observed in the Gstm1, Hmox, and Sod1 genes with the levels of AST and ALT.

Estimating Functional Groups in Human Gut Microbiome With Probabilistic Topic Models

2012 ◽  
Vol 11 (3) ◽  
pp. 203-215 ◽  
Author(s):  
Xin Chen ◽  
TingTing He ◽  
Xiaohua Hu ◽  
Yanhong Zhou ◽  
Yuan An ◽  
...  
Keyword(s):  
Functional Groups ◽  
Gut Microbiome ◽  
Topic Models ◽  
Human Gut ◽  
Human Gut Microbiome ◽  
Probabilistic Topic Models

scholarly journals Sex-related differences in local and whole-body heat loss responses: Physical or physiological?

2014 ◽  
Vol 39 (7) ◽  
pp. 843-843
Author(s):  
Daniel Gagnon
Keyword(s):  
Sex Differences ◽  
Heat Loss ◽  
Heat Production ◽  
Experimental Studies ◽  
Metabolic Heat Production ◽  
Whole Body ◽  
Fixed Rate ◽  
The Third ◽  
Metabolic Heat ◽  
Body Heat

The current thesis examined whether sex differences in local and whole-body heat loss are evident after accounting for confounding differences in physical characteristics and rate of metabolic heat production. Three experimental studies were performed: the first examined whole-body heat loss in males and females matched for body mass and surface area during exercise at a fixed rate of metabolic heat production; the second examined local and whole-body heat loss responses between sexes during exercise at increasing requirements for heat loss; the third examined sex-differences in local sweating and cutaneous vasodilation to given doses of pharmacological agonists, as well as during passive heating. The first study demonstrated that females exhibit a lower whole-body sudomotor thermosensitivity (553 ± 77 vs. 795 ± 85 W·°C−1, p = 0.05) during exercise performed at a fixed rate of metabolic heat production. The second study showed that whole-body sudomotor thermosensitivity is similar between sexes at a requirement for heat loss of 250 W·m−2 (496 ± 139 vs. 483 ± 185 W·m−2·°C−1, p = 0.91) and 300 W·m−2 (283 ± 70 vs. 211 ± 66 W·m−2·°C−1, p = 0.17), only becoming greater in males at a requirement for heat loss of 350 W·m−2 (197 ± 61 vs. 82 ± 27 W·m−2·°C−1, p = 0.007). In the third study, a lower sweat rate to the highest concentration of acetylcholine (0.27 ± 0.08 vs. 0.48 ± 0.13 mg·min−1·cm−2, p = 0.02) and methacholine (0.41 ± 0.09 vs. 0.57 ± 0.11 mg·min−1·cm−2, p = 0.04) employed was evidenced in females, with no differences in cholinergic sensitivity. Taken together, the results of the current thesis show that sex itself can modulate sudomotor activity, specifically the thermosensitivity of the response, during both exercise and passive heat stress. Furthermore, the results of the third study point towards a peripheral modulation of the sweat gland as a mechanism responsible for the lower sudomotor thermosensitivity in females.

scholarly journals RESULTS OF MULTIVARIATE EXPERIMENT TO DETERMINE DEPENDENCE OF THE MAXIMUM PRESSURE OF TRACTOR WHEELS ON THE SOIL FROM TRACTOR’S PARAMETERS AND PHYSICAL AND MECHANICAL PROPERTIES OF SOIL. STATISTICAL ANALYSIS

2017 ◽  
pp. 94-98
Author(s):  
Хафизов ◽  
Kamil Khafizov ◽  
Хафизов ◽  
Ramil Khafizov
Keyword(s):  
Mechanical Properties ◽  
Similarity Theory ◽  
Experimental Studies ◽  
Similarity Criterion ◽  
Statistical Processing ◽  
Physical And Mechanical Properties ◽  
Multiple Correlation Coefficient ◽  
Similarity Criteria ◽  
Maximum Pressure ◽  
Regression Equations

A similarity criteria were reveled to determine the dependence of maximum pressure of the tractor wheel on the soil on the basis of the use of similarity theory. Each similarity criterion includes several parameters, characterizing the tractor, its propeller and physical and mechanical properties of the soil. The similarity criteria is taken as factors in the multivariate experiment. Multifactorial experimental studies were planned and carried out. We used Statistics Toolbox subsystem of Matlab computer mathematics system for processing the results of experiments. As a result of statistical processing of the experimental data by the equation we obtained a dependence of maximum pressure of the tractor wheel on the soil from tractor parameters, and physical and mechanical properties of the soil. Statistical data on the equations, such as - multiple correlation coefficient, Fisher’s criterion, Student’s criterion, indicate the significance of equations of the experiments variants, as well as the significance of the coefficients of the regression equations.

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