scholarly journals FEED CONSUMPTION, REPRODUCTIVE FUNCTIONS, RUMEN METABOLISM, DIGESTIVENESS AND NITROGEN BALANCE IN HIGHLY PRODUCTIVE COWS IN THE SECOND PERIOD OF LACTATION FOR THE FERTILIZATION OF MIXED- LIGAND ZINC, MANGANESE AND COBALT COMPLEXES

Author(s):  
Yu. G. Kropyvka ◽  
V. S. Bomko ◽  
S. P. Babenko

The presented indicators of feed consumption, reproduction, scar fluid, digestibility of nutrients and average daily balance of Nitrogen in highly productive cows of Holstein, Ukrainian Black-Spotted dairy breed and Ukrainian Red-Spotted dairy breeds in the second period of lactation by feeding them different doses of mixed-ligand complexes of Zinc, Manganese and Cobalt. Consumption of feed mixture by experimental animals ranged from 46.2 kg to 52.2 kg. It was best consumed by cows of the 5th experimental group, in the feed mixture of which the concentration of trace elements in 1 kg of DM was: Zinc – 35 mg, Manganese – 35, Cobalt – 0.4, Selenium – 0.3, Copper – 10 mg and Iodine – 0.9 mg. It is set up that the use of different levels of mixed-ligand complexes of Zinc, Manganese and Cobalt reduces the duration of the service period in cows compared with its average value for the herd, which is 145.6 days. In the rumen fluid of experimental cows, the pH value was increasing in the alkaline direction and the difference to the control group was statistically significant. It also decreased the content of total nitrogen compared to control, which indicates its better absorption into the blood. At the same time, the content of protein and ammonia nitrogen in the rumen fluid of experimental cows was significantly reduced. The use in the rations of cows in the second 100 days of lactation of different levels of mixed-ligand complexes of Zinc, Manganese and Cobalt did not show a significant influence on the formation of volatile fatty acids in the rumen fluid, however, there was a tendency to increase them at lower concentrations of these trace elements. Cows of the experimental groups better digested dry and organic matter, crude protein, crude fat, crude fiber and NFES. The recommended and accepted norms of the dose of microelements used in the control group did not show the highest influence on the coefficients of digestibility of nutrients in feed mixtures compared with their lower doses in the experimental groups. It was found that the increase in the transformation of Nitrogen into the milk protein of cows of the experimental groups was contributed by better digestibility and less excretion of Nitrogen in the urine, which is one of the main factors in increasing their milk productivity. The elimination of zinc, manganese and cobalt deficiency in the ration of high-yielding cows due to lower doses of their mixed-ligand complexes has a positive influence on the digestibility of nutrients in the ration, nitrogen metabolism and productivity of animals.

Author(s):  
Yu. G. Kropyvka ◽  
V. S. Bomko

The article presents data on milk productivity, reproductive function and chemical composition of milk, as well as hematological parameters of blood and rumen liquid in high-yielding Holstein cows of German selection, Ukrainian black-spotted dairy and Ukrainian red-spotted dairy breeds in the first lactation period for feeding them different doses of mixed-ligand complexes of Zinc, Manganese and Cobalt. It is established that the use of mixed-ligand complexes of Zinc, Manganese and Cobalt in the feed mixture in comparison with their sulfates has a positive effect on milk productivity and reproductive function of cows and the chemical composition of their milk, and feed costs decrease with increasing productivity. The use of different forms and levels of Zinc, Manganese and Cobalt in the diets of experimental cows affected protein and carbohydrate-fat metabolism and the best results were obtained when using different levels of these trace elements in the feed due to their mixed-ligand complexes. In the rumen liquid of experimental cows, the pH value increased in the alkaline direction and the difference to the control group was statistically significant. There was also a tendency to increase residual nitrogen compared to control. There was a significant difference in the reduction of ammonia nitrogen in the rumen liquid of cows of experimental groups, which indicates better use and assimilation of crude protein. The best indicators of milk productivity, chemical composition of milk, reproductive function, blood and rumen liquid were obtained when using mixed-ligand complexes of Zinc, Manganese and Cobalt in the feed mixture when the concentration of these trace elements in 1 kg of DM was, mg: Zinc – 60.8; Manganese – 60.8; Cobalt – 0.78; Selenium – 0.3; Copper – 12 and Iodine – 1.1. This concentration of trace elements was achieved due to their mixed-ligandent complexes, copper – its sulfate, iodine – potassium iodide, selenium – selenium suplex.


Author(s):  
Yu. G. Kropyvka ◽  
V. S. Bomko

The results of studying the influence of different levels of mixed-ligand complexes of Zinc, Manganese and Cobalt in feeding high-lyproductive cows Ukrainian Black-Spotted dairy breed on feed consumption, milk productivity for the first 100 days of lactation, reproductive function and hematological parameters are presented. It was found that on average during the first 100 days of lactation experimental cows consumed 52.6-54.2 kg of feed mixture day and most of it was eaten by cows of the 4th experimental group. The best results in milk productivity (milk yield and fat and protein content) were obtained from cows of the 4th experimental group, which received rations with mixed-ligand complexes of Zinc, Manganese and Cobalt, the concentration of which in 1 kg of DM feed mixture, mg: Zinc - 48.6; Manganese - 48.6 and Cobalt - 0.62. Feed consumption per 1 kg of milk was the lowest in the 4th experimental group and amounted to 7.42 MJ of metabolic energy against 7.74 MJ of metabolic energy in the 1st control group and 7.19-7.48 MJ of metabolic energy in other experimental groups. Different levels of mixed-ligand complexes of Zinc, Manganese and Cobalt in feed mixtures of dry cows in the last 30 days of gestation caused a difference in live weight of calves at birth and had a positive effect on the reproductive functions of experimental cows. 2.1 fertilizations were required for one fruitful insemination of each cow in the 1st control group, 1.8 in the 2nd; 3rd - 1.6; 4th - 1.5 and 5th - 1.7. Analysis of hematological parameters of experimental cows shows that different levels of mixed-ligand complexes of Zinc, Manganese and Cobalt in concentrated feeds have a positive effect on the body of lactating cows, which, in turn, improves their milk productivity and especially reproductive functions.


Author(s):  
Yu. G. Kropyvka ◽  
V. S. Bomko ◽  
S. Y. Kropyvka

The article deals with the results of research on the economic efficiency of the use of mixed ligand complexes of Zinc, Manganese and Cobalt in the feeding rations of highlyproductive cows of Ukrainian Red-Spotted Dairy breed in the first 100 days of lactation. The research was performed on five groups (one control and four experimental) of analogous cows in the conditions of ALC “Terezyne” Bila Tserkva district of Kyiv region. The control was the optimal dose of mixed ligand complexes of Zinc, Manganese and Cobalt, which was previously established with a concentration of 1 kg of dry matter (DM) of the feed mixture (FM), mg: Zinc – 60.8; Manganese – 60.8 and Cobalt – 0.78. For the 2nd experimental group, the concentration of these trace elements was increased by 10 %, and in the 3rd, 4th and 5th experimental groups – on the contrary, the concentration of Zinc, Manganese and Cobalt in 1 kg of DM of feed mixture was reduced by 10 %, 20 and 30 %, respectively, compared with the control. The best results on milk yield of natural and basic fat content were got from cows of the 4th experimental group, where the concentration of Zinc, Manganese and Cobalt due to their mixed ligand complexes in 1 kg of DM of feed mixture was, mg: Zinc – 48.6; Manganese – 48.6; Cobalt – 0.62. The cost of products got from experimental cows ranged from UAH 12702.3 up to UAH 13783.7 and it was the highest in the 4th experimental group. It was set up that for cows of Ukrainian Red-Spotted Dairy breed in the first 100 days of lactation the most economically advantageous was the dose of mixed ligand complexes, in which the concentration of Zinc and Manganese was 48.6 mg, and Cobalt – 0.62 mg/kg DM, which allowed to obtain a profit of 14.37 % more than in the control group. Doses of mixed ligand complexes with a concentration of Zinc and Manganese – 66.9 mg; 54.7 and 42.6 mg, and Cobalt – 0.86 mg; 0.7 and 0.55 mg/kg CF gave a much lower economic effect. Additional profit in the 2nd experimental group amounted to UAH 66.8 or 1.34 %; in the 3rd – UAH 95.6 or 1.92 % and in the 5th – UAH 561.6 or 11.28 %, compared with the control group.


2018 ◽  
Vol 8 (1) ◽  
pp. 420-425 ◽  
Author(s):  
V. Bomko ◽  
Yu. Kropyvka ◽  
L. Bomko ◽  
S. Chernyuk ◽  
S. Kropyvka ◽  
...  

<p><span lang="EN-US">To realize the genetic potential of animals, an important role should be taken to prevent the presence of mineral substances in the rations. Because of their lack of revenue in the body of animals the productivity is decreased, the reproductive function is disturbed, there is a disease. there are diseases.</span></p><p><span lang="EN-US">Material for the scientific and economic experiment on the use of mixed ligand complexes of Zinc, Manganese and Cobalt on the milk productivity of high-yielding cows and the exchange of Mangan in their body were Holstein animals, Ukrainian Black- Spotted dairy and Ukrainian Red - Spotted dairy breeds. The trace elements were introduced into feed concentrates, and they into the fodder mix by the method of weight dosing and multi-stage mixing. Balancing of rations of highly productive cows of the 1st control group to norm only Selenium, Kuprum and Iodine, led to a concentration in 1 kg DM (dry matter) of feed mix, mg: Zinc – 32.4; Manganese – 27.8; Cobalt – 0.27; Selenium – 0.3; Copper – 12 and Iodine – 1.1 and the lowest concentration of Mangan in milk and in the body. The cows of the 2nd and 3rd experimental groups were fed with all the trace elements to the norm (for the 2nd experimental group at the expense of zinc sulfates, manganese, cobalt, copper, selenium, Suplex of Selenium and potassium iodide, for the 3rd instead of zinc sulfates, manganese and mixed cobalt compounds were introduced into their ligand complexes). The concentration of trace elements in these groups in 1 kg of DM was, mg: Zinc – 76; Manganese – 76; Cobalt – 0,97; Copper – 12; Selenium – 0.3 and Iodine – 1.1. The concentration of Zinc, Manganese and Cobalt in 1 kg of DM of fodder was reduced by 20% for cows of the 4th experimental group and by 30% for the 5th experimental group.</span></p><p><span lang="EN-US">The results of the conducted searches indicate that the use in feeding of high-yielding cows of various doses of mixed ligand complexes of Zinc, Manganese, and Cobalt on the use of Suplex of Selenium and sulfate copper and potassium iodide in the first 100- days lactation positively affect the growth of their milk production. Animals of experimental groups dominated cows of analogues of the control group, rations of which were scarce on Zinc, Manganese and Cobalt, by average daily yield of natural milk, respectively, by 4.5; 5.8 and 4.0 kg, or 11.63: 14.99 and 10.34%, and these differences were reliable. While the average daily milk yield of cows of the 2nd experimental group exceeded the average daily yield of the 1st control group by 3.8 kg or by 9.82%. In milk of experimental cows, a single increase in the fat content of 0.01–0.09% was also noted. </span></p><p><span lang="EN-US">Carrying out balance research confirmed the data of milk productivity. Thus, in the body of cows of the 3rd, 4th and 5th experimental groups, more Manganese were deposited, respectively by: 540.7; 531.5, and 394.5 mg compared to control and by 367.1, 357.9, and 220.9 mg – in comparison with the 2nd experimental group; in experimental cows of groups 4 and 5, the manganese was deposited less by 9.2 and 146.2 mg in comparison with the 3rd experimental group. With milk in cows of the 1st control group during the day was allocated 20.8 mg of Manganese, and in the 2nd, 3rd, 4th and 5th experimental groups it was allocated more, respectively by 6.7, 9.8, 15.4, and 10.6 mg. Manganese fecal excretion was less in comparison with the 2nd experimental group, in the cows of the 3rd experimental group it was less by 365.9 mg, in the 4<sup>th</sup> group – by 623.3 mg, and in the 5<sup>th</sup> group – by 691.4 mg.</span></p><p><span lang="EN-US">The use of mixed ligand complexes of Zinc, Manganese and Cobalt enhances the milk productivity of high-yielding cows, reduces their need for these elements, improves their digestion and reduces their excretion with feces.</span></p>


Author(s):  
Yu. G. Kropyvka ◽  
V. S. Bomko

The article deals with the results of research to study the digestibility of nutrients in the ration and metabolism of Nitrogen, Zinc and Manganese in high-yielding Holstein cows of German selection in the first period of lactation for feeding mixed ligand complexes of Zinc, Manganese and Cobalt. The research was performed in the conditions of Terezyne Ltd., Bila Tserkva district, Kyiv region. There was an increase in the coefficients of digestibility of dry matter in cows of the 2nd-5th experimental groups compared with animals of the 1st control group, respectively by 1.8–3.5 % (P < 0.05); organic matter by 0.3–2.5 %; crude protein by 0.5–4.2 % (P < 0.05 – P < 0.01); crude fat by 1.1–1.8 %; crude fiber by 1.3–3.3 % and nitrogen-free extractives by 2.1–4.2 %. Cows of the experimental groups consumed more Nitrogen than the cows of the control group: 20.18 g or 2.4 % – the 2nd group, 55.06 g or 6.6 % – the 3rd, 42.52 g or 5, 1 % – 4th and 28.98 g or 3.5 % – 5th experimental group due to better eating of the feed mixture. General consumption level of Nitrogen in relation to excreted with milk and deposited in the body of cows of the control group was 32.02 %, and in animals of the 2nd, 3rd, 4th and 5th experimental groups – 33.59 %; 37.31; 36.99 and 34.97 %. In relation to the total digested amount, the proportion of Nitrogen deposited in the body and excreted with milk in animals of the control group was 42.38 %, and in the experimental – 44.25–47.34 %. Doses of Zinc, which provided its existing rate of 54–85 % due to the mixed ligand complex, positively influenced on the the metabolism of trace elements in the organism of experimental cows. Assimilation of Zinc in the organism was determined by the amount of its secretions with milk and deposits in the body and in absolute terms it was the lowest in cows of the 4th and 5th experimental groups – 562.7 mg and 671.6 mg against 740.8 mg in the 1st control group and the largest – 784.7 mg and 801.3 mg in animals of the 2nd and 3rd experimental groups. The use of a mixed ligand complex of manganese ensured its positive balance in the organism of cows and the absorption of the trace element in the organism of experimental cows ranged from 22.5 % in the 2nd experimental group to 33.2 % in the 3rd experimental group. The best indicators for the metabolism of Nitrogen, Zinc and Manganese were got in the third experimental group whose cows were fed a feed mixture, that in 1 kg of DM contained, mg: Zinc – 54.7; Manganese – 54.7; Cobalt – 0.7; Selenium – 0.3; Copper – 12 and Iodine – 1.1.


2021 ◽  
Vol 9 (2) ◽  
pp. 87-92
Author(s):  
V. S. Bomko ◽  
Yu. G. Kropyvka

The results of establishing the optimal feeding doses of Zinc, Manganese, and Cobalt in the form of their mixed-ligand complexes for high-yielding cows of Holstein, Ukrainian Red-Spotted and Ukrainian Black-Spotted dairy breeds during the late dry period were presented, their influence on the future productivity and reproductive qualities of cows were also determined. It was found that lower doses of mixed-ligand complexes of Zinc, Manganese, and Cobalt in the feed mixture stimulated its consumption better. The cows of the 4th experimental group consumed more of the feed mixture, where the concentration of Zinc and Manganese was 35 mg, and Cobalt – 0.49 mg per 1 kg of DM. At various doses of mixed-ligand complexes of Zinc, Manganese, and Cobalt, the increase in live weight in cows of the control group was 24.3 kg, and in the experimental groups, it ranged from 23.7 kg to 26.9 kg, which is 8.6% and 10.7% (P < 0,05) more in the 3rd and 4th groups compared with the control. A decrease in the concentration of mixed-ligand complexes of Zinc, Manganese, and Cobalt in the diets of dry cows in the experimental groups by 10, 20, 30 and 40% had a positive effect on their further milk productivity. During the first 100 days of lactation, they milked 60-180 kg of milk more compared to the control with an increase in fat content by 0.01–0.05%. Also, in the milk of experimental group’s cows, compared with the control, the protein content increased (3.22–3.23% versus 3.14%), and the feed costs per 1 kg of milk in them were lower by 3.5–9.4%. Smaller doses of zinc, manganese, and cobalt contributed better to lengthening the period of prolonged action of their mixed-ligand complexes on the body of lactating cows. The calves’ live weight at the birth in the 4th experimental group exceeded the indicator of their peers from the control group by 2.3 kg or 8.81%, while the calves obtained from cows of the 2nd experimental group prevailed the control by 1.6 kg, or 6, 13 %, from the 3rd experimental group – by 1.8 kg, or 6.90%, and the 4th experimental group – by 1.7 kg, or 6.51%. The duration of the service period in all experimental groups of cows was within the recommended norms and was 87.8 days in the control group, and the 2nd, 3rd, 4th and 5th experimental groups, respectively 84.7 days, 82.2; 80.6 and 83.5 days, respectively, and there was less in control by 3.1–7.1 days or 3.3–8.2%. The insemination index in the cows’ control group was 1.5, and in the experimental one – 1.2–1.4, which is 6.7–20.0% less.


1970 ◽  
pp. 179-190
Author(s):  
Y. H. Kropyvka ◽  
V. S. Bomko

Purpose. To study of the effectiveness of feeding high-yielding cows in the last 100 days of lactation of different levels and sources of zinc, manganese and cobalt and establish their impact on milk productivity, feed digestibility and nitrogen balance. Methods. Five groups of highly productive cows (one control and four experimental) of Holstein, Ukrainian red-spotted and Ukrainian black-spotted dairy breeds were selected for the experiment at “Terezyne” farm, Bila Tserkva district, Kyiv region. Experimental cows were fed the same rations. The difference was in different doses of mixed ligand complexes of trace elements in the premix. The control was the optimal dose of mixed-ligand complexes of zinc, manganese and cobalt, which was established in a previous experiment with a concentration of 1 kg of dry matter (DM) of feed, mg: zinc – 50, manganese – 50 and cobalt – 0.78. Results. It was found that the use of different doses of mixed ligand complexes of zinc, manganese and cobalt using Suplex selenium and copper sulfate and potassium iodide in feeding cows in the last 100 days of lactation have differently affected their milk productivity. If 2320 kg of milk was milked from each cow of the control group during this period, then from cows of the 2nd-5th experimental groups – 40-220 kg more. The difference in the average daily milk yield of 4% fat was 0.5-2.67 kg and it was significant (P <0.001). The results of the balance experiment show that the animals of the experimental groups better digested feed nutrients and absorbed nitrogen. Thus, in cows of the 2nd, 3rd, 4th and 5th experimental groups nitrogen was deposited in the body respectively by 2.1, 4.1, 7.3 and 12.8 g more, and it was statistically significant (P <0,05) compared with animals of the control group. Conclusions. The use of mixed ligand complexes of zinc, manganese and cobalt helps to increase milk productivity of cows, reduce feed consumption per 1 kg of product and reduces the need of animals for these elements. The best performance results were obtained in the 4th experimental group, the animals of which were fed with a feed mixture containing 1 kg of DM, mg: zinc – 35; manganese – 35; cobalt – 0.4; selenium – 0.3; copper – 9 and iodine – 0.8.


Author(s):  
Yu. G. Kropyvka ◽  
V. S. Bomko

A number of scientific publications on the influence of microelements on the vital activity of cows, the level of milk productivity and reproductive function are analyzed. The article presents the results of research on the study of different levels of mixed-ligand complexes of Zinc, Manganese and Cobalt in the feeding of cows in the dry season for their future milk productivity and reproductive functions. The research was carried out in the conditions of “Terezine”, Bila Tserkva district, Kyiv region, on highly productive cows of the Ukrainian black-spotted dairy breed. It was found that the elimination of Zinc, Manganese and Cobalt deficiency in the diets of cows in the dry season due to different doses of their mixed-ligand complexes had a positive effect on further milk productivity. In terms of gross milk yield of natural milk for milk of 4 % fat content, the difference in this indicator between the cows of the 2nd experimental group and control was 195.9 kg, or 5.52 % (P < 0.01), the 3rd experimental – 220.4 kg, or 6.21 % (P < 0.001), the 4th experimental – 242.0 kg, or 6.82 % (P < 0.001) and the 5th experimental group and control – 116.3 kg, or 3.28 %. Different concentrations of mixed-ligand complexes of the studied micronutrients in the diets of dry and lactating cows differently affected their reproductive functions. One fertile insemination of each cow in the 1st control group required 2.8 inseminations, in the 2nd, 3rd, 4th and 5th experimental groups – 2.1; 1.9; 1.7 and 2.3 fertilization, which is 75.0 according to the control; 67.9; 60.7 and 82.1 %. Lower levels of Zinc, Manganese and Cobalt led to positive dynamics in the physiological state of cows, stimulated their hunting and provided normal conditions for the processes of fertilization and embryo development in cows. The best effect on the milk productivity of cows and their reproductive functions had the following levels of trace elements in 1 kg of SR feed, mg: Zinc – 30; Manganese – 30; Cobalt – 0.38; Selenium – 0.3; Copper – 9 and Iodine – 0.7. This concentration of trace elements was achieved due to their mixed-ligand complexes, and Copper – its sulfate, Iodine – potassium iodide, and Selenium – selenium Suplex.


1970 ◽  
pp. 146-159
Author(s):  
Yu.H. Kropyvka ◽  
V.S. Bomko

Purpose. To study the effectiveness of feeding different levels of mixed-ligande complex of zinc, manganese and cobalt with Suplex Se to high-yielding cows of the Ukrainian red-spotted dairy breed in the first period of lactation and their impact on feed consumption, productivity and hematological parameters of blood. Methods. Five groups (one control and four experimental) of highly productive cows of the Ukrainian red-spotted dairy breed, 10 heads each, were selected for the research at «Terezyne» farm, Bila Tserkva district, Kyiv region, on the principle of analogues. Experimental cows were fed the same rations. For cows of the control group the optimal dose of a mixed-ligande complex of Zinc, Manganese and Cobalt was used, which was established in a previous experiment with concentration in 1 kg of dry matter (DM) of feed, mg: zinc – 60.8; manganese – 60.8 and cobalt – 0.78. In the 2nd experimental group, concentration of those trace elements was higher compared to the control by 10%, and in the 3rd, 4th and 5th experimental groups, respectively, 10, 20 and 30% lower. Results. The best results in terms of milk productivity were obtained from cows of the 4th experimental group. For 80 days of the experiment from cows of the 1st control group 3080 kg of milk of natural fat content was obtained, and of the 2nd, 3rd, 4th and 5th experimental groups – respectively 48, 112, 200 and 136 kg, or by 1.6; 3.6; 6.5 and 4.4% more. Lower doses of micronutrients meet the needs of highly productive cows in them, which has a positive effect on the reproductive functions of animals. The most effective was the dose for the cows of the 4th experimental group, where duration of the service period was 78.6 days against 91.9 days in the 1st control group, and one fertile insemination in this group took 1.8 times at 3.1 times in control. Analysis of hematological parameters of the experimental cows shows that different levels of mixed-ligande complexes of zinc, manganese and cobalt in concentrated feeds have a positive effect on the body and health of lactating cows. Conclusions. The best results of milk productivity and reproductive function were obtained from cows of the 4th experimental group, where concentration of zinc, manganese and cobalt due to their mixed-ligande complexes in 1 kg of DM feed was, mg: zinc – 42.6; manganese – 42.6; cobalt – 0.55. This dose of trace elements had a positive effect on the consumption of animal feed, their unit cost and hematological parameters of cows' blood.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 405-405
Author(s):  
Vinícius C De Souza ◽  
Juliana Messana ◽  
Erick Batista ◽  
Paulo Henrique Colovate ◽  
Maria Júlia Ganga ◽  
...  

Abstract The goal of this study was to evaluate the effects of different levels (10 and 13% crude protein) and protein source on ruminal fermentation of feedlot Nellore steers fed high-concentrate diets. The protein sources tested were urea, dried distillers grains (DDG) and corn gluten meal. The DDG and gluten were used as RUP sources. We used 6 cannulated Nellore steers, rumen, duodenum, and ileum arranged in 6×6 Latin square designed in factorial (2 nitrogen levels and 3 protein sources) balanced for residues. The pH was measured immediately after rumen fluid sampling at 0, 2, 4, 6, 8 and 10h after feeding. The NH3-N concentrations were determined with micro-Kjeldahl apparatus. The concentration of volatile fatty acids (VFA) was assessed by gas chromatography. Data were evaluated using the MIXED procedure of SAS, with repeated measures over time. There was no interaction between sampling time and treatments for NH3-N and pH (P &gt; 0.05). Animals fed diets containing Urea had a higher concentration of NH3-N (20.6 mg/dL) than animals supplemented with Gluten (16.2 mg/dL) or DDG (12.7 mg / dL). There was an increase in the NH3-N concentration (P = 0.049) with the increase of the protein level 10 to 13% CP with 14.7 and 18.3 mg/dL, respectively. The ruminal pH was affected by the dietary protein level (P = 0.016), in which animals fed a level of 10% CP had a higher pH (6.80) compared to the level of 13% CP (6.43). Total and individual VFA concentration was not affected by protein levels and sources (P &gt; 0.05). The reduction of N level and the use of RUP did not adversely affect the ruminal fermentation. Although significant differences were observed for pH, they were small and adequate for microbial protein synthesis in the rumen.


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