scholarly journals ACTIVITY OF MALATE-ASPARTATE SHUTTLE ENZYMES OF BULLS AND BOARS REPRODUCTIVE ORGANS AND EPIDIDIMAL SPERMATOZOA

2020 ◽  
Vol 21 (2) ◽  
pp. 86-89
Author(s):  
N. V. Kuzmina ◽  
D. D. Ostapiv ◽  
O. I. Chajkovska ◽  
R. D. Ostapiv ◽  
O. P. Panych
Keyword(s):  
Malate Dehydrogenase ◽  
Aspartate Aminotransferase ◽  
Protein Concentration ◽  
Tissue Specificity ◽  
Testicular Tissue ◽  
Reproductive Organs ◽  
The Body ◽  
White Breed ◽  
Dairy Breed ◽  
Malate Aspartate Shuttle

The activity of malate-aspartate shuttle enzymes in the reproductive organs and epididimal sperm of bulls and boars was studied. The research was conducted on bulls of the Ukrainian black-spotted dairy breed (n = 5; aged 14 - 16 months) and boars of the Great White breed (n = 3; aged 10 - 12 months). After slaughter, the testes and testicular appendages were removed and the spermatozoa were washed with 0.9% sodium chloride solution. Testicular and epididimal tissues were homogenized and centrifuged. Aspartate aminotransferase (AST), malate dehydrogenase (MDG) and protein concentration were determined in the supernatant and epididimal sperm. The specie and tissue specificity of aspartate aminotransferase and malate dehydrogenase activity have been established. In particular, in bulls, the activity of AST in the tissues of the testis, head and body of the epididymis of bulls is almost the same (116.5 - 118.3 nmol/min×mg of protein) and on 18.5 - 19.7% (р<0,05) higher in the tail of the appendix. In this case, MDG activity in the reproductive organs shows wavy changes: in the testicular tissue is high (1.96 ± 0.15 nmol / min × mg of protein), and in the epididymis: in the head is reduced (3.5 times ; p <0,001), increased in the body (p <0,05) and decreased again in the tail. In the sperm of bulls, the activity of AST from the head of the appendix - 69.3 ± 8.06 nmol / min × mg of protein, from the body - increased (p <0.05) and again (p <0.001) decreased from the tail of the appendix. MDG activity in sperm from the head of the epididymis was 0.50 ± 0.04 nmol/min×mg of protein, lower by 38.0% (p <0.05) from the body and 50.0% (p <0.001) higher in the tail. In boars, the activity of AST in testicular tissue - 20.3 ± 5.22 nmol / min × mg of protein and higher in the appendix: 45.6% - in the head, 59.0% - in the body and 64.0 % - in the tail. Similarly, the activity of MDG in testicular tissue was 0.22 ± 0.02 nmol / min × mg of protein, increases by 40.6% in the head and remained at the same level in the body of the appendix, and in the tail tissue above 2, 5 - 2.7 times (p <0.001). In bovine germ cells, the activity of AST and MDG from the body of the appendix was, respectively, 102.3 ± 6.1 and 13.20 ± 0.15 nmol / min × mg of protein, lowered twice (p <0,001) and 10.7 % in the head and more than four times (p <0,001) and 61,2% (p <0,001) in the tail of the appendix.

Distributions of aspartate aminotransferase and malate dehydrogenase activities in rat retinal layers.

1985 ◽  
Vol 33 (7) ◽  
pp. 624-630 ◽  
Author(s):  
C D Ross ◽  
D A Godfrey
Keyword(s):  
Energy Metabolism ◽  
Correlation Coefficient ◽  
Malate Dehydrogenase ◽  
Aspartate Aminotransferase ◽  
Horizontal Cell ◽  
Plexiform Layer ◽  
The Other ◽  
Retinal Layers ◽  
Cell Processes ◽  
Malate Aspartate Shuttle

Aspartate aminotransferase (AAT), an enzyme interconverting glutamate and aspartate, has been suggested to be a marker for glutamatergic and/or aspartatergic neurons. However, AAT, glutamate, and aspartate are also involved in cellular metabolism, e.g., the malate-aspartate shuttle. To investigate the extent to which AAT might be involved in these several functions in retina, the distribution of AAT activity in rat retinal layers was compared to that of malate dehydrogenase (MDH), an enzyme of aerobic metabolism proposed to be physically complexed with AAT in the malate-aspartate shuttle mechanism. The distribution of AAT activity in retinal layers closely paralleled that of MDH (correlation coefficient AAT versus MDH = 0.93). AAT activity was proportionately higher than MDH in the photoreceptor inner segments, containing a high density of mitochondria, and in the outer plexiform layer (OPL), containing photoreceptor terminals and bipolar and horizontal cell processes. The amount of total AAT activity in the inner segments related to the mitochondrial isoenzyme is almost twice that in the other layers tested, including the OPL. The correlation between AAT and MDH activities is consistent with AAT involvement in retinal energy metabolism, although other functions, such as neurotransmission, are possible.

scholarly journals Biochemical characterization of two sibling species in the liverwort Pellia epiphylla (L.) Corda (Hepaticae, Pelliaceae)

2014 ◽  
Vol 67 (1) ◽  
pp. 83-86
Author(s):  
Wiesław Prus-Głowacki ◽  
Roman Zieliński ◽  
Jerzy Szweykowski
Keyword(s):  
Malate Dehydrogenase ◽  
Sibling Species ◽  
Aspartate Aminotransferase ◽  
Soluble Protein ◽  
Protein Concentration ◽  
Biochemical Characterization ◽  
Relative Activity

Two sibling species in the <em>Pellia epiphylla</em> (L.) Corda (n=9) complex, viz. <em>Pellia epiphylla</em> sp. <em>N</em> and <em>Pellia epiphylla</em> sp. <em>S</em> were compared in their protein concentration and in the dynamics and also in relative activity of some enzymes: malate dehydrogenase (MDH), esterase (EST), peroxidase (PX) and aspartate aminotransferase (GOT). Serological properties of their antigens were studied as well. Activity of malate dehydrogenase, esterase and aspartate aminotransferase was significantly higher in clons of <em>Pellia epiphylla N </em>than <em>Pellia epiphylla S</em>. Some differences in soluble protein concentration and in the quantities of the AI to AIV antigens between the two sibling species were detected.

Purification and properties of the cytosolic and mitochondrial forms of aspartate aminotransferase and malate dehydrogenase from rat heart

1987 ◽  
Vol 65 (3) ◽  
pp. 239-244 ◽  
Author(s):  
Charalampos Mavrides ◽  
Guylaine Nadeau
Keyword(s):  
Malate Dehydrogenase ◽  
Crude Extract ◽  
Aspartate Aminotransferase ◽  
Rat Heart ◽  
Pyridoxal Phosphate ◽  
Single Procedure ◽  
Purification And Properties ◽  
Michaelis Constants ◽  
Malate Aspartate Shuttle ◽  
Original Crude

The present work describes the purification from rat heart of the mitochondrial and cytosolic forms of the enzymes of the malate–aspartate shuttle, aspartate aminotransferase (EC 2.6.1.1) and malate dehydrogenase (EC 1.1.1.37), by a single procedure after the preparation of the original crude extract. In 10 purification steps, the four enzymes were obtained electrophoretically pure in yields ranging from 6 to 54% of their respective isoenzyme levels in the crude extract. Apoenzymes were formed from the aminotransferases by reacting them with cysteine sulfinate and dialyzing. Complete reconstitution was obtained after a brief incubation with pyridoxal phosphate. All four enzymes are dimers. The mitochondrial isoenzymes are of slightly lower molecular weight than their respective cytosolic forms. Michaelis constants and maximal velocities were derived by the use of primary and secondary plots. In general, the properties of the enzymes from rat heart are similar to the properties of the enzymes from other animal sources.

Linear type trait evaluation of firstborns of Ukrainian Black-and-White Dairy breed depending on the body build

2019 ◽  
pp. 24-34
Author(s):  
R. Stavetska ◽  
Y. Dynko ◽  
M. Bushtruk ◽  
I. Starostenko ◽  
O. Babenko ◽  
...  
Keyword(s):  
Body Condition Score ◽  
High Capacity ◽  
The Body ◽  
Body Depth ◽  
Linear Type ◽  
Body Build ◽  
Black And White ◽  
Condition Score ◽  
Type Traits ◽  
Dairy Breed

It has been experimentally proven that the strength and direction of the connection between the linear type traits evaluation of the Ukrainian black-and-white dairy breed cows and their body build depends on the type of the build and the sign of the exterior. The study was conducted in 2018 on a breeding plant of the Ukrainian Black-and-White Dairy breed SPC named Shchorsa in Kiev region (n=89). Differentiation of cows on types of their body build was conducted by the method proposed by Chernenko (low-capacity, mid-capacity and high-capacity types) and Kolesnik (by the massiveness index – on the friable and dense, the bone index – delicate and rough, the wide-breast and wide-rump indices – on narrow-body and wide-body types). It was found that the cow-heiferswere well-developed, had intermediate stature and optimum body condition score, deep body, and wide, slight slope rump. They alsohad desirable rear legs, set and foot angle but some toe-out, the udder was well-balanced. Under the good total score of the exterior of studied cows there were some animals with short stature (134– 136 cm), very wide rump, straight rear legs side view and toe-out, extremely steep foot angle, loose fore udder attachment, deep udder below hocks and front teat placement inside of quarter. The degree of development of the studied linear traits of cow-heifers confirms their in-herd variability. The variability of the exterior traits varies from 10,7 % (body depth) to 38,6 % (stature). In addition to the stature of a significant variability was characterized of angularity (22,4 %), rear legs set (22,5 %), body condition score (21,8 %), as well as traits that describe udder: rear udder attachment (31,4 %), udder depth (32,6 %) and front and rear teat position (31,3 % and 25,1 %, respectively). The high variability of linear traits indicates the possibility of effective their selection. As a result of the research, it has been established that the strength and direction of the correlations between the linear type traits evaluation of Ukrainian Black-and-White Dairy breed cows and the type of their body build depends on the type of the body build and the linear trait. The low-, mid- and high-capacity bodies build types (according to Chernenko) reliably correlate with chest width (r=0,58) and body depth (r=0,48), rear legs rear view (r=0,30), the central ligament (r=0,28), udder depth (r =-0,22) and teat length (r=0,25), P˂0,01–0,001. Friable and dense bodies build (according to Kolesnik) were most strongly associated with stature (r=-0,28), chest width (r =-0,70), and body depth (r=-0,38), as well as with rear legs rear view (r=-0,23), P˂0,05...0,001; delicate and rough bodies build – with stature (r=-0,51), angularity (r=-0,22),rump width (r=-0,35), udder depth (r=-0,27) and rear udder attachment (r =0,20), P ˂ 0,05–0,001; narrow-body and wide-body types – with chest width (r=0,30) and rump width (r=0,23), P˂0,05–0,01. Linear type trait evaluation of cows depends on the body build. The strength and direction of the correlations between types of body build and linear traits showed that the most informative is the classification of the body build by low-, mid- and high-capacity according to Chernenko – on the average η2x=17,5 %. The less informative – by narrow-body and wide-body types according to Kolesnik – η2x=7,6 %. The classification of cows on delicate-rough, narrow-body and wide-body types (according to Kolesnik) according to informativeness and reliability can have the intermediate position – η2x=10,0–11,5 %. The strongest influence on the body build has the cows’ height (η2x=27,9 % on average for all studied types of body build), chest width (η2x=33,5 %), body depth (η2x=21,8 %), rump width (η2x=24,1 %) and rear legs (η2x=18,7 %). Key words: cows, linear type trait evaluation, body build, correlation, power of influence.

Reproductive Organ Education for Psychic Health Development of Early Age

2018 ◽  
Vol 2 ◽  
Author(s):  
Dwi Darwati
Keyword(s):  
Early Childhood ◽  
Early Childhood Development ◽  
Deviant Behavior ◽  
Reproductive Organs ◽  
Reproductive Organ ◽  
The Body ◽  
Childhood Development ◽  
Early Age ◽  
Digital Era ◽  
Stage Of Development

Reproductive  health education should be given since early childhood by using language that is adapted to the stage of development. If you procrastinate and wait until the teenager it is already too late because in the days of the digital era, as now, all the information can be easily accessed by anyone including children early age. If the early childhood misinformed about their reproductive organs it would disrupt the physical and psychological development due to the wrong behavior in caring for and maintaining reproductive organs. Qur’an as the holy book of Muslims describes the steps of reproduction and  imparting education wisely as well as how to apply such education. This kind of education must be in accordance with the conditions of children and there should not be a lie about it We can also use media and methods such as pictures, songs, tap or other visual  media which can give clearer information, so that children can clearly see parts of the body, their characteristics, and how to treat and care them. The impropriate approach in conveying this kind of knowledge will be very dangerous for children. The provision of early age reproductive organs education can prevent the occurrence of deviant behavior as well as protect children from dangerous influence in early childhood development.

FEATURES OF THE EXTERIOR TYPE OF UKRAINIAN BLACK-AND-WHITE DAIRY BREED OF THE CHERKASSY REGION, ESTIMATED BY THE LINEAR CLASSIFICATION METHOD

2017 ◽  
Vol 54 ◽  
pp. 112-119
Author(s):  
L. M. Khmelnychy
Keyword(s):  
Local Population ◽  
The Body ◽  
Breeding Value ◽  
Linear Classification ◽  
Body Structure ◽  
Linear Type ◽  
Maternal Line ◽  
Black And White ◽  
Type Traits ◽  
Dairy Breed

The estimation of the first-born cows of Ukrainian Black-and-White dairy breed by the exterior type in the leading breeding herds of Cherkassy region by the method of linear classification (n = 566) has been carried out. The results of the evaluated animals by the 100-score system indicate that within the group of traits, the average level of assessment is "good with the plus". The animals have been differed by the good development of group features characterizing the dairy type (82.4 score), body condition (83.5 score), limbs (82.8 score), udder (82.7 score) and overall score (82.8 score). The degree of development 18 descriptive traits of the exterior of cows, has been predicted by the method of linear classification, indicates their significant intraherd variability. Animals have the moderate height, deep body and angularity. The rump angle, the angle of hock joint and the teats length, according to the variability of scores in the range of 17.8–22.4%, have an average optimal development. The level of scores for the rear width (5.9 score), attachment of the fore (6.3 score) and the rear parts (6.0 score) of the udder testifies to their good development. The body structure of the cows-firstborn has a sufficient description of the type traits that determine their milk yield. At the present stage of selection and breeding work due to the use of bull-sires different origins and breeding value on the local population livestock with significant genotype diversity, the degree of influence each of the hereditary factors in the overall variability of linear features has a scientific and practical significance. In our studies, the force of influence conditional bloodiness of the Holstein mother's breed in the overall fraction of variability of linear type traits within the exterior complexes and the overall assessment takes a significant percentage - from 24.9 to 31.5 with a high degree of reliability according to Fischer's criterion. Among the descriptive traits, the proportion of influence the conditional bloodiness of mother, which is confirmed by high statistical confidence, ranges from 14.4 to 19.8%, and refers to the traits important for breeding. The level of coefficients of influence the father's breeding value in the total phenotypic variability for some of the linear traits is high and reliable. First of all it concerns group traits of the exterior, the development of which is determined by the pedigree value of father by 37.1–41.8%, and the overall assessment of type – by 46.6%. The coefficients of influence father's breeding value in most of the descriptive traits have high reliability (P < 0,001). The coefficients of the force of influence father, depending on the heredity of Holstein for the linear traits of almost the same level as his pedigree value. Since in this case the organized factor is purebreed Holstein and crossbreed bulls-sires of domestic selection, the positive role of Holstein heredity in improving the exterior type of cows has been traced realistically. The necessity of linear breeding, as an effective method in the system of dairy cattle breeding, has been substantiated by reliable values of the coefficients of the force of influence of father's line on the linear traits of offspring (3.4–29.5%). Despite the significant variability of coefficients of the force of influence on the linear traits, most of them have a high level of reliability within the evaluated body parts important for selection. The effect of cows belonging to the maternal line on the development of features of the body structure is insignificant and unreliable (5.3–9.2%). In the process of breeding aimed at improving cows of dairy herd by the exterior type, the pedigree value of parents has been assessed according to the method of linear classification of their daughters and their belonging to perspective genealogical formations.

The internal anatomy of the woodlouse, Metoponorthus pruinosus (Brandt), (Porcellionidae, Peracarida)

1968 ◽  
Vol 46 (3) ◽  
pp. 321-327 ◽  
Author(s):  
M. A. Alikhan
Keyword(s):  
Nervous System ◽  
Digestive System ◽  
Circulatory System ◽  
Reproductive Organs ◽  
The Body ◽  
Internal Anatomy ◽  
Suboesophageal Ganglion ◽  
Digestive Tube ◽  
Thoracic Ganglia ◽  
Digestive Glands

Tbe circulatory system, lying in the mid-dorsal line of the body, consists of an oval heart, the opthalmic artery, and a dorsal abdominal artery.The digestive system comprises a wide, large alimentary tube and two pairs of digestive glands. An oesophagus, a proventriculus, midgut, and a short proctodacum or hindgut form the digestive tube. The digestive glands are very well developed and are beaded in form; each pair lies on either side of the alimentary canal.The reproductive organs are well developed in both sexes: in the male they consist of paired testes and their vas deferentia, and in the female paired bilobed ovaries and oviducts.A cerebral or supraoesophageal ganglion, a suboesophageal ganglion, and seven thoracic ganglia form the nervous system. The supraoesophageal ganglion is united with the suboesophageal ganglion by means of the circumoesophageal commissures, whereas the thoracic ganglia and suboesophageal ganglia are linked with each other by paired connectives.The gills and the tracheae are the organs of respiration. The gills are borne of the bases of the pleopods and are enclosed in the branchial chamber. The tracheae are located on the lateral lobes of the first two pleopods only.

scholarly journals CHANGES IN THE VOLUME OF PLASMA AND ABSOLUTE AMOUNT OF PLASMA PROTEINS IN NEPHRITIS

1924 ◽  
Vol 39 (6) ◽  
pp. 921-929 ◽  
Author(s):  
G. C. Linder ◽  
C. Lundsgaard ◽  
D. D. Van Slyke ◽  
E. Stillman
Keyword(s):  
Plasma Protein ◽  
Plasma Volume ◽  
Plasma Proteins ◽  
Protein Concentration ◽  
The Body ◽  
Absolute Amount ◽  
Low Protein

1. We have not observed gross increases in plasma volume in glomerulonephritis, nephrosis, or nephrosclerosis, even when the concentration of plasma proteins was much below normal. Our results indicate the probability that "hydremic plethora" does not occur. 2. The low protein concentration frequently observed in the plasma in nephritis is not due to increased plasma volume but to a decrease of the total amount of plasma protein in the body. 3. Changes in plasma volume showed no constant relationship to changes in edema.

scholarly journals EFFICIENCY OF THE USE THE CROSSBREEDING OF UKRAINIAN BLACK-AND-WHITE DAIRY AND SWISS CATTLE IN THE STATE STUD FARM "PASICHNA"

2021 ◽  
Vol 61 ◽  
pp. 35-48
Author(s):  
I. V. Verbych ◽  
O. V. Medvid
Keyword(s):  
Functional Properties ◽  
The Body ◽  
Natural Resistance ◽  
Adaptation Capacity ◽  
Black And White ◽  
Dairy Fat ◽  
Service Period ◽  
Effective Selection ◽  
High Level ◽  
Dairy Breed

Goal. To study the influence of intermediate crossing on the level of dairy productivity, qualitative milk indicators, exterior features and resistance of the body of pureorgain and local animals. Methods. Selection and genetic analysis, comparative, statistical. The results. Scientific and production studies were conducted on the basis of a tribal factory of the State Enterprise "Pasichnya" IKSGP NAAN "of the StarosinyaVsky district of the Khmelnytsky region in the chains of distinct animals of the Podilsky factory type of Ukrainian Black-and-White dairy (UCHRMP) and local animals derived from the crossing of the BPRMP cows with Bojabs of the Shvitsky breed. According to the results of experimental studies, it has been found that local cows-first-bristles are somewhat inferior to the purest animals of the Ukrainian Black-and-White dairy breed by the magnitude of the NADA (93.8 kg), but it is compensated by milk quality (fat +0.26%, protein +0.19%), where in the general yield of dairy fat (+9.5 kg) and protein (+6.4 kg), they are favorably different from its purgatory rior. The analysis of the results of the reproductive capacity of cows shows that local animals were first fruitfully inseminated at the age of 545 days and the duration of fertility was 283 days, at that time, purely meters were inserted at the age of 567 days, and the duration of the sharing was 281.5 days. Service-period in the cows-first-born of genotypes, respectively, amounted to 97 and 112 days. The obtained data for the morphological and functional properties of the cow-first-prints show that the assessed number of both groups meets the needs of target standards on technological features and have high indicators of the studied functional properties. Comparative analysis of exterior valuation indicators between the studied groups shows that local cows have higher rates over latitudinal gates, in particular, by breast width and width in the ice, at that time inferior to the children's rior in the rectors of height in the racing, in the area of the torso. and hammer. In the study of natural resistance in the studied cows it has been established that the estimated animal groups are characterized by a sufficiently high level of protective functions of the body and adaptation capacity to technological conditions, which creates good opportunities for further effective selection. The general assessment of the natural resistance of cows-firstbody by morphological and biochemical parameters of blood, phagocytic, bactericidal and lysozymic activity showed that local cows according to Method VE Chumachenko and others. (1990) have a natural resistance to 3 points (61) higher than in their pure-breeding rior (58 points). Conclusions. Results of analysis of dairy performance of local cows-first-birthsters derived from the crossing of Ukrainian Black-and-White breeds with bulls of the Shvitskaya breed showed that the data of the animal though inferior to the christening rior of the Ukrainian Black-and-White dairy breed by the magnitude of the NADA but this difference is compensated by the quality of milk, where The total output of dairy fat and protein, they are favorably different from pureoral analogues. By indicators of reproducible ability, it has been found that local animals were first crazy at the age of 823 days that on 21 days earlier than puredom and 15 days they have a smaller service period. An analysis of the results of the estimation of morphological and physiological properties of the elder showed that there are no significant differences in the investigated groups of primary differences. All animals correspond to technological requirements. The exterior evaluation of the investigated groups of the firstborn showed that purely cows, having higher rates in the elevation in the roll, in the height in the ice and a contrary length of the trunk and the intensity, but inferior to the latitude gauge: the width of the breast, width in the machaches and width in the machaches and width in the machach. Animal estimation according to natural resistance indicators found that animals of both groups have a sufficiently high level of protective functions of the body and adaptation capacity to technological conditions that creates good opportunities for further effective selection.

scholarly journals EFFECT OF THE DRUG "E-SELENIUM" ON THE DISPERSION OF MILK FAT IN COW'S MILK

2021 ◽  
Vol 245 (1) ◽  
pp. 71-76
Author(s):  
L.G. Kashirina ◽  
◽  
K.I. Romanov ◽  
Keyword(s):  
Milk Fat ◽  
Live Weight ◽  
The Body ◽  
Quantitative Composition ◽  
Black And White ◽  
Fat Globules ◽  
White Breed ◽  
Total Fat ◽  
The Relationship ◽  
Experimental Group

The antioxidant drug "E-selenium" was used to reduce the processes of LPO in the body of fresh cows. Two groups were formed from the analogous cows of the black-and-white breed: The Control was intact, Experienced received the antioxidant drug injection at a dose of 10 ml at the rate of 1 ml per 50 kg of live weight per head for 4 months of lactation from the second. The diets of animals of all groups were the same, balanced in nutrients and met the standards of the RAAS. The duration of the experiment was 150 days. During the period from the 3rd to the 5th month of lactation, there was a positive dynamic in increasing the number of fat globules in the milk of cows of the Experimental group. By the end of the 4th month, it was higher than the Control by 8.9 %. This confirms the active effect of the drug "E-selenium", which contributes to a positive dynamic in increasing the size of fat globules. Non-enzymatic antioxidant tocopherol is an active element of the anti-oxidative system. Selenium stimu-lates the body's own antioxidant system. In both experimental groups, the relationship between the concentration of peroxidation products and the quantitative composition of fat globules and their size was established. In the milk of cows of the Experimental group, the oxidation processes were inhibited by neutralizing the products (POL), increasing the number of large fat globules and the total fat content in the milk.

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