The design and usage of a portable incubator for inexpensive in-field water analysis

Humanitarian engineers need an inexpensive, fast, visually compelling way to assess bacterial water quality in remote locations. One way to do this is with 3M Petrifilm E. coli/Coliform (EC) Count Plates to detect E. coli in water samples. These require incubation at close to body temperature. To meet this need, we provide a free, open-source design of a battery-powered incubator capable of maintaining 35°C ± 1°C for up to 65 hours in ambient temperature of 25°C. Our incubator, called the Armadillo, can be replicated by an ordinarily skilled person in five hours for under USD $200.00 in materials cost. This paper summarises the reference documentation for construction, sample handling, inoculation, and incubation using Petrifilms and the Armadillo. Colony-forming unit (CFU) counts generated by the Armadillo are compared side-by-side with a laboratory-grade incubator. Incubation performance at ambient temperatures of 25°C and 4°C shows that a single battery charge reliably powers a full incubation period of 48 hours under normal ambient temperatures.

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STUDI PERBANDINGAN KUALITAS AIR BERSIH DALAM KAITANYA DENGAN AKTIVITAS MASYARAKAT DI SEKITAR SUMBER AIR PADA DESA WAINITU, BATUMERAH, AMAHUSU, DAN HALONG

JURNAL BUDIDAYA PERTANIAN ◽

10.30598/jbdp.2017.13.2.111 ◽

2017 ◽

Vol 13 (2) ◽

pp. 111-119

Author(s):

Lela Uyara ◽

Pieter Kunu ◽

Silwanus M Talakua

Keyword(s):

Water Quality ◽

Quality Standard ◽

Total Coliform ◽

Quality Analysis ◽

Clean Water ◽

Water Quality Analysis ◽

E Coli ◽

Microbiological Parameters ◽

Standard Quality

The study aims to determine the quality of clean water in the villages of Wainitu, Batumerah, Amahusu and Halong by comparing the result of water quality analysis with water quality standard. Water quality analysis includes Physiscal, Chemical, and Microbiological parameters. This research uses descriptive method, this method describes systematics, accurate about facts and characteristic of the quality of clean water of each research location. The results showed that the source of clean water in the village of Batumerah did not meet the standard of clean water quality standards indicated by the number of E. coli and the high total coliform. Keywords: standard quality of clean water, water quality, Wainitu, Batumerah, Amahusu and Halong villages ABSTRAK Penelitian yang bertujuan untuk menetapkan kualitas air bersih di Desa Wainitu, Batumerah, Amahusu dan Halong, dengan membandingkan hasil analisis kualitas air dengan standar baku mutu air bersih. Analisis kualitas air meliputi parameter fisika, kimia dan mikrobiologi. Penelitian ini menggunakan metode deskriptif; metode ini menggambarkan sicara sistematis, akurat, fakta dan karakteristik mengenai kualitas air bersih di masing-masing lokasi penelitian. Hasil penelitian menunjukkan bahwa sumber air bersih di Desa Batumerah tidak memenuhi standar baku mutu air bersih yang ditunjukkan oleh jumlah E. coli dan total Koliform yang tinggi. Kata Kunci: baku mutu air bersih, Desa Wainitu, Batumerah, Amahusu dan Halong, kualitas air

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Water Quality of Swimming Pools in Jerusalem

Water Science & Technology ◽

10.2166/wst.1993.0562 ◽

1993 ◽

Vol 27 (7-8) ◽

pp. 287-294 ◽

Cited By ~ 2

Author(s):

S. Lerman ◽

O. Lev ◽

A. Adin ◽

E. Katzenelson

Keyword(s):

Water Quality ◽

Fecal Coliforms ◽

Pathogenic Microorganisms ◽

Swimming Pools ◽

Indicator Organisms ◽

Indicator Microorganisms ◽

E Coli ◽

Safe Water ◽

Indirect Information

The Israel Ministry of Health is now revising its regulations for the assurance of safe water quality in public swimming pools. Since it is not possible to monitor each of the pathogenic microorganisms, it is often recommended to monitor indicator bacteria which provide indirect information on the water quality in the swimming pool. Three indicator microorganisms are often recommended: coliform counts (total coliforms, fecal coliforms or E. Coli), staphylococcus aureus and pseudomonas aeruginosa. A four year survey of the water quality of swimming pools in the Jerusalem District was conducted in order to determine whether the monitoring of all three indicators is necessary to assure safe water quality or is it sufficient to monitor only a single microorganism. A statistical analysis, conducted by using several different statistical techniques, reveals that the populations of the three indicator organisms are significantly interdependent but the correlations between each pair of these indicators are not sufficient to base a prediction of any of the organisms based on the measurements of the others. Therefore, it is concluded that monitoring of all three indicators should be recommended in order to provide an adequate picture of the water quality in swimming pools.

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The relationship between health effects in triathletes and microbiological quality of freshwater

Water Science & Technology ◽

10.2166/wst.1995.0549 ◽

1995 ◽

Vol 31 (5-6) ◽

pp. 19-26 ◽

Cited By ~ 7

Author(s):

G. J. Medema ◽

I. A. van Asperen ◽

J. M. Klokman-Houweling ◽

A. Nooitgedagt ◽

M. J. W. van de Laar ◽

...

Keyword(s):

Water Quality ◽

Geometric Mean ◽

Microbiological Quality ◽

Quality Parameters ◽

Health Complaints ◽

E Coli ◽

Thermotolerant Coliforms ◽

Microbiological Water Quality ◽

The Relationship ◽

Faecal Streptococci

This pilot study was carried out to determine the relationship between microbiological water quality parameters and the occurrence of health complaints among triathletes. Data were collected at an Olympic distance triathlon (n=314) and a run-bike-run (n=81; controls for exposure to fresh water). At the time of the triathlon, the concentrations of Escherichia coli , thermotolerant coliforms, faecal streptococci, entero- and reoviruses, F-specific RNA phages, Salmonella, Campylobacter, Aeromonas, Plesiomonas shigelloides, Pseudomonas aeruginosa and Staphylococcus aureus were examined over the swimming course. Information on the occurrence of health complaints during the competition and in the week thereafter was collected through a written questionnaire. The results show that triathletes and run-bike-runners are comparable with respect to factors other than water exposure (age, sex, training history, physical stress, lower intestinal health complaints during the competition) that may influence the occurrence of health complaints in the week after the competition. Triathletes and run-bike-runners reported gastro-intestinal (7.7% vs 2.5%), respiratory (5.5% vs 3.7%), skin/mucosal (2.6% vs 1.2%), general (3.5% vs 1.2%) and total symptoms (14.8% vs 7.4%) in the week after the event. The health risks for triathletes for all symptom groups are not significantly higher than for run-bike-runners. The geometric mean concentration of faecal indicator bacteria is relatively low: E. coli 170/100 ml; faecal streptococci 13/100 ml, enteroviruses were present at concentrations of 0.1/l. The group of triathletes was homogeneusly and relatively intensely exposed to water; they all swam in the same body of water at the same time and 75% reported to have swallowed freshwater. It was concluded that this study design is suitable to study the relationship between health complaints and microbiological water quality. In the summers of 1993 and 1994, a study will be carried out concerning several run-bike-runs and triathlons in freshwaters of different quality.

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The effect of type of water supply on water quality in a developing community in South Africa

Water Science & Technology ◽

10.2166/wst.1997.0706 ◽

1997 ◽

Vol 35 (11-12) ◽

pp. 35-40 ◽

Cited By ~ 6

Author(s):

B. Genthe ◽

N. Strauss ◽

J. Seager ◽

C. Vundule ◽

F. Maforah ◽

...

Keyword(s):

Water Quality ◽

Water Supply ◽

Cross Sectional Study ◽

Cross Sectional ◽

E Coli ◽

Guideline Values ◽

Developing Communities ◽

Quality Guideline ◽

And Control ◽

The Relationship

Efforts to provide water to developing communities in South Africa have resulted in various types of water supplies being used. This study examined the relationship between the type of water supply and the quality of water used. Source (communal taps, private outdoor and indoor taps) and point-of-use water samples were examined for heterotrophic plate counts (HPC), total and faecal coliforms, E. coli, and coliphages. Ten percent of samples were also analysed for enteric viruses, Giardia and Cryptosporidium. Approximately 320 households were included in a case-control study. In addition, a cross-sectional study was conducted. Both studies examined the relationship between different types of water facilities and diarrhoea among pre-school children. The source water was of good microbial quality, but water quality was found to have deteriorated significantly after handling and storage in both case and control households, exceeding drinking water quality guideline values by 1-6 orders of magnitude. Coliphage counts were low for all water samples tested. Enteric viruses and Cryptosporidium oocysts were not detected. Giardia cysts were detected on one occasion in case and control in-house samples. Comparisons of whether in-house water, after handling and storage, complied with water quality guideline values demonstrated households using communal taps to have significantly poorer quality than households using private outdoor or indoor taps for HPC and E. coli (χ2 = 14.9, P = 0.001; χ2 = 6.6, P = 0.04 respectively). A similar trend (although not statistically significant) was observed for the other microbial indicators. The cross-sectional study demonstrated an apparent decrease in health risk associated with private outdoor taps in comparison to communal taps. This study suggests that a private outdoor tap is the minimum level of water supply in order to ensure the supply of safe water to developing communities.

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KUALITAS AIR SUNGAI DI KAWASAN WISATA PANTAI PETITENGET, KEROBOKAN KABUPATEN BADUNG BALI

ECOTROPHIC Jurnal Ilmu Lingkungan (Journal of Environmental Science) ◽

10.24843/ejes.2018.v12.i02.p11 ◽

2018 ◽

Vol 12 (2) ◽

pp. 226

Author(s):

Wayan Budiarsa Suyasa ◽

Sri Kunti Pancadewi G. A ◽

Iryanti E. Suprihatin ◽

Dwi Adi Suastuti G. A.

Keyword(s):

Water Quality ◽

Structural Changes ◽

Pollution Index ◽

Basic Structure ◽

Physical Damage ◽

E Coli ◽

Pollutant Source ◽

Quality Category ◽

Index Value ◽

The Impact

In order to maintain the environmental carrying capacity of coastal tourism, this research was conducted to determine the condition of river water environmental pollution in the Petitenget beach area and pollutant source activities. Determination of water quality is carried out by analyzing the water quality taken at several sampling points in the four rivers that lead to the Petitenget beach. Determined the pollution index value (IP) of the physical chemical and biological pollution parameters. The results showed that the four rivers that flow into the Petitenget Beach area had been contaminated with indications of pH, BOD, COD, ammonia, Coliform and E. coli which exceeded water quality category III class quality (PerGub Bali No 16 Year 2016). The four rivers are included in the criteria of severe contamination. The four rivers have experienced physical damage or structural changes that have very high discharge fluctuations both in quantity and quality. Slimy basic structure, smelly and slum aesthetic waters. While the indication of the impact of pollution is waste water which is directly discharged into the river from hotels, restaurants, homestays, commercial centers and settlements.

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Turntable Paper-Based Device to Detect Escherichia coli

Micromachines ◽

10.3390/mi12020194 ◽

2021 ◽

Vol 12 (2) ◽

pp. 194

Author(s):

Yung-Chih Wang ◽

Yao-Hung Tsai ◽

Ching-Fen Shen ◽

Ming-Yao He ◽

Yi-Chen Fu ◽

...

Keyword(s):

Escherichia Coli ◽

Infectious Diseases ◽

Point Of Care ◽

Multiple Integral ◽

Enzyme Linked Immunosorbent Assay ◽

Considerable Time ◽

E Coli ◽

Colony Forming Unit ◽

Experimental Implementation ◽

Colony Forming Units

Escherichia coli has been known to cause a variety of infectious diseases. The conventional enzyme-linked immunosorbent assay (ELISA) is a well-known method widely used to diagnose a variety of infectious diseases. This method is expensive and requires considerable time and effort to conduct and complete multiple integral steps. We previously proposed the use of paper-based ELISA to rapidly detect the presence of E. coli. This approach has demonstrated utility for point-of-care (POC) urinary tract infection diagnoses. Paper-based ELISA, while advantageous, still requires the execution of several procedural steps. Here, we discuss the design and experimental implementation of a turntable paper-based device to simplify the paper-based ELISA protocols for the detection of E. coli. In this process, antibodies or reagents are preloaded onto zones of a paper-based device and allowed to dry before use. We successfully used this device to detect E. coli with a detection limit of 105 colony-forming units (colony-forming unit [CFU])/mL.

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Drinking water quality and antibiotic resistance of E. coli and Salmonella spp. from different sources in Gweru urban, Zimbabwe

Environmental Monitoring and Assessment ◽

10.1007/s10661-021-09322-5 ◽

2021 ◽

Vol 193 (8) ◽

Author(s):

Desmond Tichaona Mugadza ◽

Sibusisiwe Isabel Nduku ◽

Edlyn Gweme ◽

Sherpherd Manhokwe ◽

Patience Marume ◽

...

Keyword(s):

Water Quality ◽

Drinking Water ◽

Antibiotic Resistance ◽

Drinking Water Quality ◽

Salmonella Spp ◽

E Coli ◽

Different Sources

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Structural Analysis and Optimization of Hybrid Unmanned Amphibian Aerial Vehicle

International Journal of Vehicle Structures and Systems ◽

10.4273/ijvss.13.2.14 ◽

2021 ◽

Vol 13 (2) ◽

Author(s):

C. Gajendran ◽

M.K. Padmanabhan ◽

M. Dinesh ◽

S. Vignesh ◽

S. Rajesh ◽

...

Keyword(s):

Water Quality ◽

Structural Analysis ◽

Water Quality Management ◽

Bathymetric Survey ◽

Operational Conditions ◽

Remote Locations ◽

Hydrographic Survey ◽

Aerial Vehicle ◽

Unmanned System ◽

Related Application

Hybrid Unmanned Amphibian Aerial Vehicle (HUAAV) is a hybrid unmanned system consisting of a multi-rotor and a hovercraft. Water quality management, hydrographic survey, bathymetric survey and related application areas still employs time consuming and erroneous traditional methods of using boats to access the test sites. The application of unmanned systems will significantly increase the efficiency of this process by getting access to remote locations of both air and water. This paper deals with the design optimization and finite element structural analysis to validate the structural capability of the hybrid amphibian aerial vehicle for operational conditions. The Reserve Factors (RF) for all the components in the amphibian aerial vehicle are calculated and then optimized.

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Flood and rainfall mobilisation of E. coli and faecal source tracking markers from decomposing cowpats

10.5194/egusphere-egu21-3577 ◽

2021 ◽

Author(s):

Megan Devane ◽

Brent Gilpin ◽

Jennifer Webster-Brown ◽

Louise Weaver ◽

Pierre Dupont ◽

...

Keyword(s):

Water Quality ◽

Overland Flow ◽

Quantitative Polymerase Chain Reaction ◽

Linear Regression Analysis ◽

Water Quality Monitoring ◽

Quality Monitoring ◽

Source Tracking ◽

Faecal Contamination ◽

E Coli ◽

Faecal Indicators

The intensification of dairy farming on the agricultural landscape in New Zealand has raised concerns about pollution sources from dairy faecal runoff into waterways. The transport of faecal pollution from farms into waterways is facilitated by overland flow, which can result from rain and flood events, poorly designed irrigation practices and the washing down of milking sheds.An important step for mitigation of pollution is the identification of the source(s) of faecal contamination. When elevated levels of faecal indicator bacteria (FIB) such as Escherichia coli are identified in a waterway, faecal source tracking (FST) tools such as microbial source tracking (MST) using quantitative polymerase chain reaction (qPCR), and faecal steroids (for example, cholesterol) provide information about the sources of faecal contamination. The understanding of the fate (degradation/persistence) and transport of these FST markers in the environment is recognised as an important requirement for the interpretation of water quality monitoring in aquatic environments.This study investigated the effects of faecal decomposition on bovine faecal indicators (E. coli and FST markers: bovine-associated qPCR markers and ten faecal steroids) by monitoring the effect of flood and rainfall events on simulated cowpats over a five and a half month period under field conditions. Two separate spring/summer trials were conducted to evaluate: Trial 1) the mobilisation under simulated flood conditions of the faecal indicators from irrigated versus non-irrigated cowpats, Trial 2) the mobilisation of faecal indicators from non-irrigated cowpat flood runoff versus runoff after simulated rainfall onto non-irrigated cowpats.The microbial community changes within the decomposing cowpat (as illustrated by amplicon-based metagenomic analysis) were expected to impact on the survival/persistence of the bacterial targets of the MST markers, and also alter the ratio between faecal sterols and their biodegradation products, the stanols. It was hypothesised, therefore, that there would be:<ul><li>Changes over time in the concentration of E. coli and the bovine-associated MST markers mobilised into the cowpat runoff</li> <li>Alterations in the FST ratio signature of the ten measured faecal steroids, resulting in a change from a bovine faecal steroid signature in fresh cowpat runoff to other animal faecal signatures in the runoff from decomposing cowpats</li> <li>A difference in the mobilisation decline rates of all FST and microbial markers within a treatment regime and between treatments.</li> </ul>Linear regression analysis was undertaken to establish mobilisation decline rates for each of the analytes in the mobilisable phase from the cowpat runoff treatments, with calculation of the time taken in days for reduction in 90% of the concentration (T90), and statistical comparison of the regression coefficients (slopes) of all analytes. The results will include a discussion of the impacts of the study&#8217;s observations on the interpretation of faecal indicator assessments for water quality monitoring in waterways influenced by sources of faecal contamination.

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Effects of Water Quality and Post-Harvest Handling on Microbiological Contamination of Lettuce at Urban and Peri-Urban Locations of Ouagadougou, Burkina Faso

Foods ◽

10.3390/foods7120206 ◽

2018 ◽

Vol 7 (12) ◽

pp. 206 ◽

Cited By ~ 6

Author(s):

Juliane Dao ◽

Kathrin Stenchly ◽

Oumar Traoré ◽

Philip Amoah ◽

Andreas Buerkert

Keyword(s):

Water Quality ◽

Irrigation Water ◽

World Health ◽

Vegetable Production ◽

Salmonella Spp ◽

Total Coliforms ◽

E Coli ◽

Post Harvest ◽

Health Organization ◽

Production Areas

Vegetable production in urban gardens of Ouagadougou contributes to food security, but water for irrigation is often of low quality. This is particularly acute if irrigation water is taken from wastewater polluted channels. This study aimed at (i) verifying to what degree irrigation water quality is correlated with contamination of lettuce with Escherichia coli, total coliforms, and Salmonella spp., and (ii) assessing effects of post-harvest handling on pathogen development during the trade chain. We tested pathogen removal efficiency on lettuce by applying post-harvest washing. Irrigation water of production areas in Ouagadougou (n = 10) showed a mean E. coli load of 2.1 × 105 CFU 100 mL−1. In 60% of the cases, irrigation water did not meet the standards of the World Health Organization (WHO) for safe irrigation water, and in 30% of the cases, irrigation water was contaminated with Salmonella spp. Loads of total coliforms on lettuce leaves ranged from 2.9 × 103 CFU g−1 to 1.3 × 106 CFU g−1, while E. coli averaged 1.1 × 102 CFU g−1. Results on post-harvest handling revealed that microbial loads increased along the trade chain. Overall, half of all lettuce samples (n = 60) were tested positively for Salmonella spp. The experiment showed that appropriate post-harvest handling could prevent the increase of total coliforms.

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