scholarly journals CUSTO DE DIFERENTES FONTES DE NITROGÊNIO EM CULTIVO DE MICROALGA COM MEIO FERTILIZANTE

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Stela Valenti Raupp ◽  
Paulo Abreu
Keyword(s):  
Nannochloropsis Oculata ◽  
Thalassiosira Weissflogii

Os fertilizantes agrícolas são utilizados como nutriente para a produção massiva de microalgas. Vários meios de cultura utilizam amônio e uréia como fonte de nitrogênio. No entanto, análises de nutrientes indicam que as formas de nitrogênio não são igualmente utilizadas pelas diferentes espécies de microalgas, o que significa que o uso simultâneo de ambas as fontes de nitrogênio pode representar custos extras na produção. Neste estudo avaliamos qual forma de nitrogênio (amônio ou uréia) é preferencialmente assimilada pelas microalgas marinhas Nannochloropsis oculata e Conticribra (Thalassiosira) weissflogii. Os experimentos foram realizados com três tratamentos: a) Controle - com uréia e sulfato de amônio, b) Amônio - apenas com sulfato de amônio c) Uréia - apenas com uréia. Não houve diferença significativa na densidade celular entre os tratamentos com sulfato de amônio apenas e com ambas as fontes de nitrogênio (Controle) para as duas espécies. Esses dados sugerem que a remoção de uréia na produção em larga escala de microalgas não afeta o crescimento da espécie, e representam economia de 10% no preparo da solução de meio de crescimento.

Efektifitas Ekstrak Nannochloropsis oculata Terhadap Peningkatan Kepadatan Kolagen pada Proses Penyembuhan Alveolar Osteitis

DENTA ◽  
2016 ◽  
Vol 10 (1) ◽  
pp. 9
Author(s):  
Ajeng Saghita Enggardini ◽  
Syamsulina Revianti ◽  
Noengki Prameswari
Keyword(s):  
Nannochloropsis Oculata ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Alveolar Osteitis

<p><strong><em>Latar Belakang:</em></strong><em> </em><em>Alveolar osteitis merupakan salah satu komplikasi pasca</em><em> </em><em>pencabutan </em><em>gigi</em><em>.</em><em> Kondisi ini biasanya menjadi masalah pasca pencabutan yang menyebabkan rasa nyeri dan menghambat penyembuhan soket. </em><em>Ekstrak </em><em>Nannochloropsis oculata</em><em> mengandung </em><em>alkaloid, carotenoid, flavonoid, terpenoid dan tocopherol </em><em>yang dapat mempercepat proses penyembuhan luka.</em><em> </em><strong><em>Tujuan:</em></strong><em> Mengetahui efektivitas ekstrak Nannochloropsis oculata terhadap peningkatan kepadatan kolagen pada proses penyembuhan alveolar osteitis. <strong>Bahan dan Metode:</strong> </em><em>Rancangan penelitian ini adalah post test only control group design, dengan menggunakan 64 tikus Wistar jantan dibagi menjadi 2  kelompok  besar, yaitu pemberian irigasi dan dressing. Pada masing-masing kelompok terdapat kelompok kontrol negatif (irigasi PBS atau dressing PBS), kelompok kontrol positif (irigasi Saline 0,9% atau dressing Alvogyl) dan kelompok perlakuan (irigasi atau dressing ekstrak Nannochloropsis oculata 2,5% dan 5%). Kemudian ke</em><em>padatan kolagen</em><em> diukur pada hari ke 3 dan ke 7 setelah perlakuan. Ke</em><em>padatan kolagen </em><em>yang diukur pada soket pasca pencabutan gigi. Setelah dilakukan treatment pada hari ke 3 dan ke 7, semua tikus dikorbankan lalu ke</em><em>padatan kolagen </em><em>diukur melalui preparat histopatologi anatomi dengan pengecatan </em><em>Masson’s Trichom</em><em> dan pembesaran </em><em>100</em><em>x</em><em>, </em><em>kemudian dilakukan pe</em><em>rhitungan kepadatan kolagen</em><em>. Data yang diperoleh dianalisis</em><em> Kruskal-Wallis dan Mann-Whitney. </em><strong><em>Hasil:</em></strong><strong><em> </em></strong><em>Terdapat perbedaan </em><em>kepadatan kolagen </em><em>yang signifikan antara kelompok perlakuan irigasi (0,045), tetapi tidak terdapat perbedaan yang signifikan antara kelompok perlakuan dressing (0,243). Metode dressing lebih efektif dalam meningkatkan kepadatan kolagen jika dibandingkan dengan kelompok irigasi (0,026).</em><em> </em><strong><em>S</em></strong><strong><em>impulan:</em></strong><em> Pemberian dressing ekstrak Nannochloropsis oculata konsentrasi 5% paling efektif dalam meningkatkan kepadatan kolagen pada proses penyembuhan alveolar osteitis pada hari ke 7.</em><em></em></p>

STUDY AND CHARACTERIZATION GROWTH OF FOUR MICROALGAE SPECIES AND TEST ANTIMICROBIAL ACTIVITY

Jurnal Zarah ◽  
2018 ◽  
Vol 6 (2) ◽  
pp. 53-58
Author(s):  
Marniati Salim
Keyword(s):  
Dunaliella Salina ◽  
Methanol Extract ◽  
Sea Water ◽  
Basal Medium ◽  
Nannochloropsis Oculata ◽  
Inhibitory Zone ◽  
Antimicrobial Sensitivity ◽  
Sensitivity Tests ◽  
Microalgae Biomass ◽  
Chaetoceros Calcitrans

Abstract In this study to the growth characteristics of microalgae (Dunaliella salina, Nannochloropsis oculata, Tetrasel mis chuii & Chaetoceros calcitrans), in different mediums, namely Bold’s Basal Medium (BBM) and BBM modification of sea water. The results obtained from microalgae (N.oculata, T.chuii, C.calcitrans) are better grown on BBM media while microlaga D.salina grows well on BBM modification of sea water. Microalgae biomass is extracted by maceration method in hexane and methanol solvents. Test bacteria used gram positive (Staphylococcus aureus) and gram negative (Escherichia coli). Inhibitory zone diameter results were compared with antimicrobial sensitivity tests according to Clinical and Laboratory Standards Institute (CLSI). From the results of microalgae hexane and methanol extract antibacterial activity tests (D.salina, N.oculata, T.chuii, C.calcitrans) showed that the extract was not too sensitive to inhibit bacterial growth.   Keywords: microalgae, medium, antimicrobial

Isolation, morphometry, and culture of Colurella sp. (Rotifera: Ploimida)

2013 ◽  
Vol 1 (2) ◽  
pp. 143
Author(s):  
Petrus P Letsoin ◽  
Henneke Pangkey ◽  
Julius Sampekalo ◽  
Inneke F.M Rumengan ◽  
Stenly Wullur ◽  
...  
Keyword(s):  
Body Size ◽  
Marine Fish ◽  
Fish Larvae ◽  
Nannochloropsis Oculata ◽  
Tropical Fish ◽  
Sampling Location ◽  
Larval Rearing ◽  
Brachionus Rotundiformis ◽  
Marine Fish Larvae ◽  
Significant Difference

The rotifer Brachionus rotundiformis (total body length 240.59±10.24 μm, lorica length 175.28±9.18 μm, and lorica width 124.28±7.76μm) is commonly used as starter food in the larval rearing of marine fish. But, larvae of some marine tropical fish species required starter food with body size smaller than B. rotundiformis. The present study was aimed to isolate minute rotifers from nature and to assess the possibility of culturing these rotifers. Sampling of rotifers was conducted in an estuary of Mangket (Kema-Minut), using plankton net (mesh size 40 µm). A trial of culturing the rotifers was conducted at salinities of 10, 20 and 30 ppt by using a microalga, Nannochloropsis oculata. A species of rotifer identified as Colurella sp. (family Lepadellidae) was successfully isolated from the sampling location. Body size of Colurella sp. was extremely small (Total length 123.22±5.45 μm, lorica length 95.96±3.81 μm, and lorica width 53.57±3.11 μm), which were smaller than Brachionus rotundiformis SS-type as a conventional starter food for marine fish larvae.  Results of culturing the minute rotifer Colurella sp. showed that the species grew well at salinities of 10, 20 and 30 ppt with no significant difference among treatments (ANOVA, p>0.05), indicating a potential use of minute rotifer Colurellasp. as starter food for marine fish larvae. Rotifera Branchionus rotundiformis (ukuran tubuh: panjang total 240,59±10,24 μm, panjang lorika 175,28±9,18 μm, dan lebar lorika 124,28±7,76μm) sering digunakan sebagai pakan awal pemeliharaan larva ikan laut. Namun, larva beberapa spesis ikan laut tropis membutuhkan pakan awal berukuran tubuh lebih kecil dari Branchionus rotundiformis. Penelitian ini bertujuan untuk mendapatkan minute rotifer dari alam (berukuran tubuh lebih kecil dari B. rotundiformis) dan menguji kemungkinan pemeliharaannya. Sampling rotifer dilakukan di perairan estuari Desa Mangket (Kema-Minut), menggunakan plankton net (ukuran mata jaring 40 µm). Uji coba pemeliharaan dilakukan pada salinitas (10, 20, dan 30 ppt) dengan menggunakan Nannochloropsis oculata. Satu spesies minute rotifer yang teridentifikasi sebagai Colurella sp. (family Lepadellidae) berhasil diisolasi dari lokasi sampling. Colurella sp. memiliki ukuran tubuh sangat kecil (panjang total [PT] 123,22±5,45 µm, panjang lorika [PL] 95,96±3,81 µm, dan lebar lorik [LL] 53,57±3,11 µm) yang mana lebih kecil dari Branchionus rotundiformis tipe-SS sebagai pakan awal larva ikan laut. Hasil uji coba pemeliharaan minute rotifer Colurella sp. menunjukkan bahwa spesis ini dapat tumbuh pada salinitas 10, 20, dan 30 ppt dengan perbedaan kepadatan populasi yang tidak signifikan antar perlakuan (Uji ANOVA, p > 0.05) mengindikasikan potensi pemanfaatan minute rotifer Colurella sp. sebagai pakan awal larva ikan laut.

scholarly journals Full-Length Transcriptome of Thalassiosira weissflogii as a Reference Resource and Mining of Chitin-Related Genes

Marine Drugs ◽  
2021 ◽  
Vol 19 (7) ◽  
pp. 392
Author(s):  
Haomiao Cheng ◽  
Chris Bowler ◽  
Xiaohui Xing ◽  
Vincent Bulone ◽  
Zhanru Shao ◽  
...  
Keyword(s):  
Cell Wall ◽  
Full Length ◽  
Biosynthetic Pathways ◽  
Glycosidic Linkage ◽  
Thalassiosira Weissflogii ◽  
Ecological Value ◽  
Pacbio Sequencing ◽  
Non Coding Rnas ◽  
Chitin And Chitosan ◽  
Simple Sequence

β-Chitin produced by diatoms is expected to have significant economic and ecological value due to its structure, which consists of parallel chains of chitin, its properties and the high abundance of diatoms. Nevertheless, few studies have functionally characterised chitin-related genes in diatoms owing to the lack of omics-based information. In this study, we first compared the chitin content of three representative Thalassiosira species. Cell wall glycosidic linkage analysis and chitin/chitosan staining assays showed that Thalassiosira weissflogii was an appropriate candidate chitin producer. A full-length (FL) transcriptome of T. weissflogii was obtained via PacBio sequencing. In total, the FL transcriptome comprised 23,362 annotated unigenes, 710 long non-coding RNAs (lncRNAs), 363 transcription factors (TFs), 3113 alternative splicing (AS) events and 3295 simple sequence repeats (SSRs). More specifically, 234 genes related to chitin metabolism were identified and the complete biosynthetic pathways of chitin and chitosan were explored. The information presented here will facilitate T. weissflogii molecular research and the exploitation of β-chitin-derived high-value enzymes and products.

A photobioreactor using Nannochloropsis oculata marine microalgae for removal of polycyclic aromatic hydrocarbons and sorption of metals in produced water

Chemosphere ◽  
2021 ◽  
pp. 130775
Author(s):  
Isadora Machado Marques ◽  
Adna Caroline Vale Oliveira ◽  
Olivia Maria Cordeiro de Oliveira ◽  
Emerson Andrade Sales ◽  
Ícaro Thiago Andrade Moreira
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Produced Water ◽  
Nannochloropsis Oculata ◽  
Marine Microalgae ◽  
Polycyclic Aromatic

Influence of the Insecticide Diflubenzuron (Dimilin) on the Growth of Marine Diatoms and a Harpacticoid Copepod in Culture

1985 ◽  
Vol 42 (7) ◽  
pp. 1272-1277 ◽  
Author(s):  
N. J. Antia ◽  
P. J. Harrison ◽  
D. S. Sullivan ◽  
T. Bisalputra
Keyword(s):  
Skeletonema Costatum ◽  
Harpacticoid Copepod ◽  
Adult Survival ◽  
Thalassiosira Weissflogii ◽  
Marine Diatoms ◽  
Tigriopus Californicus ◽  
Per Se ◽  
Juvenile Development ◽  
Cyclotella Cryptica ◽  
Injurious Effects

Diflubenzuron (Dimilin) was tested, in the concentration range 0.1–5000 μg∙L−1, for possible injurious effects on the growth and photosynthesis of three chitin-producing (Thalassiosira weissflogii, T. norden-skioldii, Cyclotella cryptica) and one nonchitinaceous (Skeletonema costatum) diatoms. For comparison, the effects of the pesticide were also examined on adult survival and juvenile development of the harpacticoid copepod Tigriopus californicus. While the development of the copepod was hindered at concentrations of the order of 1–10 μg∙L−1, the diatoms were barely affected by Dimilin even at the highest concentration tested (5 mg∙L−1). We conclude that Dimilin acts specifically on insects and crustaceans as a larvicide by interfering with chitin deposition into cuticles during juvenile development through ecdysis. The lack of effect from Dimilin on the chitin-producing diatoms has suggested that the insecticide may not inhibit chitin biosynthesis per se as was previously believed, but that it presumably deregulates one or more of the larval postsynthetic processes responsible for chitin integration into cuticles.

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