Bacteriological Profile and Antibiogram of Neonatal Sepsis

Introduction : Neonatal sepsis remains an important cause of neonatal morbidity and mortality in NICU setup and a major challenge for the neonatologists. The prevalent organisms and their antibiotic resistance patterns evolve with time and with the usage of antimicrobials.Aims and Objectives : To analyze the bacteriological profile and antibiotic resistance patterns of proven neonatal sepsis cases in Dhaka Shishu Hospital.Materials and Methods : The study was Prospective observational study. All the clinically suspected cases of Neonatal Sepsis admitted to the Neonatal word from January 2015 to December 2015 were included in the study. Clinically suspected cases of neonatal sepsis further evaluated with blood cultures and antibiotic susceptibility testing using the Kirby Bauer disc diffusion method. Data was collected for the following variables: Demographic profile, haematological profile, blood culture result and antibiotic sensitivity patterns.Results : Total 96 cases clinically suspected neonatal sepsis were included in the study. Among them 29 cases (30.21%) was culture positive septicemia. Klebsiella pneumoniae was the most common isolate accounting for 31.03% cases followed by Escherichia coli 27.59%. In Klebsiella infection most common sensitivity were Imipenum and Ciprofloxacin which were 77.78 and 33.33% respectively. In E.coli common sensitive drugs were Imipenum and Amikacin. In serratia common sensitive drugs were Imipenum, Netlmicin and Ciprofloxacin.Conclusion : K. pneumoniae was the most common pathogen. Most common sensitivity was Imipenum, Amikacin, Ciprofloxacin, Gentamicin, Netlmicin, and Ceftazidime,.Northern International Medical College Journal Vol.8(1) July 2016: 174-177

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The Relationship of Birth Weight Infant with Bacteriological Profile and Antibiotic Resistance of Neonatal Sepsis

Asian Journal of Applied Sciences ◽

10.24203/ajas.v7i5.5932 ◽

2019 ◽

Vol 7 (5) ◽

Author(s):

Nisrina Asysyifa ◽

Husjain Djajaningrat ◽

Diah Lestari

Keyword(s):

Antibiotic Resistance ◽

Birth Weight ◽

Blood Culture ◽

Low Birth Weight ◽

Neonatal Sepsis ◽

P Value ◽

Klebsiella Pneumonia ◽

Bacteriological Profile ◽

Relationship Of ◽

The Relationship

Neonatal sepsis is a major issue on neonatal-care field. This incident occurs by many factors, one of the factor is infant with a low birth weight. Blood culture is used as the gold standard for diagnosis. The spectrum of bacteria which caused neonatal sepsis is constantly change and vary due to antibiotic resistance phenomenon. This study aimed to determine the relationship of birth weight infant with bacteriological profile and antibiotic resistance of neonatal sepsis in RSAB Harapan Kita Jakarta. This study used observational method with cross sectional design and purposive sampling method which is analyzed by chisquare test. Medical record data and blood culture and antibiotic resistance test from all subjects in Januari until December 2018 were reviewed. The sample in this research is neonatal sepsis patients who met inclusion criteria were 51 patients. From 51 (55,43%) subjects, there were 39 (76,47%) neonatal sepsis in low birth weight infant. Klebsiella pneumonia spp (41,17%) and Staphylococcus epidermidis (19,60%) were the predominant pathogens. Amikacin (62,74%), and meropenem (50,98%) were the most susceptible antibiotic towards bacteria. Maximum resistance among organisms was seen in cefotaxime (84,31%), ceftazidime (78,43%), and amoxicillin (70,58%).Â The result of the analysis found there is a relationship between birth weight infant and bacteriological profile (p-value = 0,035), but there is no relationship between birth weight infant and antibiotic resistance of neonatal sepsis (p-value =0,092; 0,066; and 0,521). There is a relationship between birth weight infant and bacteriological profile , but there is no relationship between birth weight infant and antibiotic resistance of neonatal sepsis.

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Bacteriological profile and antibiotic susceptibility of neonatal sepsis in a tertiary care hospital

International Journal of Paediatrics and Geriatrics ◽

10.33545/26643685.2020.v3.i1c.76 ◽

2020 ◽

Vol 3 (1) ◽

pp. 165-168

Author(s):

Dr. Shubhangi Kedar ◽

Dr. Anjali Parekh

Keyword(s):

Neonatal Sepsis ◽

Antibiotic Susceptibility ◽

Tertiary Care Hospital ◽

Tertiary Care ◽

Care Hospital ◽

Bacteriological Profile

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Bacteriological Profile of Neonatal Sepsis and Antibiogram of the Isolates

Journal of Nepal Paediatric Society ◽

10.3126/jnps.v37i1.16303 ◽

2017 ◽

Vol 37 (1) ◽

pp. 5-9

Author(s):

Roshan Parajuli ◽

Narayan Dutt Pant ◽

Raju Bhandari ◽

Anil Giri ◽

Suman Rai ◽

...

Keyword(s):

Staphylococcus Epidermidis ◽

Neonatal Sepsis ◽

Diffusion Method ◽

Preliminary Treatment ◽

Biochemical Tests ◽

Gram Positive ◽

Cross Sectional ◽

Gram Negative ◽

Bacteriological Profile ◽

Gram Positive Cocci

Introduction: Neonatal sepsis is a serious problem in developing countries like Nepal. The main objectives of this study were to determine the bacteriological profile of neonatal sepsis, to determine the antimicrobial susceptibility patterns of the causative agents and to evaluate the association between the neonatal sepsis and the different characteristics of the neonates.Methods: A hospital based cross-sectional study was conducted among a total of 450 neonates suspected of suffering from sepsis. Blood culture was performed using statdard microbiological techniques. The colonies grown were identified on the basis of colony morphology, Gram’s stain and biochemical tests. The antimicrobial susceptiblility testing was performed by Kirby Bauer disc diffusion method. Results: Out of total 450 blood samples, 92 (20.4%) were culture positive. Of which, 16 (17.4%) samples contained gram negative bacteria and 76 (82.6%) samples contained gram positive cocci. The most common bacterial pathogens isolated were Staphylococcus epidermidis (67.4%) followed by Escherichia coli (13%). All gram positive cocci were susceptible to vancomycin, while all gram negative bacilli were sensitive to amikacin. There was statistically significant relationship between neonatal sepsis and gestation age of neonates.Conclusions: Neonatal sepsis is still present as a serious problem in Nepal. Staphylococcus epidermidis was the most common cause of the neonatal sepsis. Prematurely delivered neonates should be given more care, as they are more prone to suffering from neonatal sepsis. Vancomycin and amikacin can be used as the drugs of choice for preliminary treatment of neonatal sepsis in our settings.

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Incidence, bacteriological profile and antibiotic sensitivity pattern of neonatal sepsis in a tertiary health facility in Abuja, North-central Nigeria.

10.21203/rs.2.21673/v2 ◽

2021 ◽

Author(s):

Olutunde Oluyinka ◽

Kareem I. Airede ◽

Kudi E. Olateju ◽

Obaro K. Stephen ◽

Nosakhare Izevbigie ◽

...

Keyword(s):

Blood Culture ◽

Incidence Rate ◽

Neonatal Sepsis ◽

White Cell Count ◽

Antibiotic Sensitivity ◽

Signs And Symptoms ◽

Special Care Unit ◽

Bacteriological Profile ◽

Sensitivity Pattern ◽

Pseudomonas Luteola

Abstract Background: Neonatal sepsis is commonly caused by bacteria in the first 28 days of life. Due to diagnostic limitations in developing settings, prompt laboratory identification of causative organisms is usually a challenge. To prevent mortality, clear knowledge of bacteria and their antibiotic sensitivity patterns are important for prompt empirical treatment. Methods: This prospective study enrolled 339 newborns with signs and symptoms suggestive of neonatal sepsis out of 645 that were admitted into the special care unit of the University of Teaching Hospital during the study period. Socio-demographic and clinical profiles of the newborns were obtained using a questionnaire and blood culture was done from every enrolled newborn (339 newborns) using BACTEC 9050. The bacteriological profile and antibiotic sensitivity pattern of newborns with confirmed neonatal sepsis were documented. Results: A total of 339 newborn were admitted for probable sepsis out of a total admission of 645 newborns during the study period based on clinical features and initial laboratory work-up. Forty-six of the 645 newborns (46/645) had culture proven sepsis resulting in a neonatal sepsis incidence rate of 71.3 (95%CI 50.7-91.9) per 1000 admitted newborns. Seventeen of the 46 confirmed sepsis cases were among the 1322 newborns delivered within the study facility during the study period giving an in-hospital neonatal sepsis incidence rate of 12.9 (95% CI 6.7-19.0) per 1000 live births. Amongst the 46 babies with positive blood culture, 27/46 (58.7%) had normal white cell count while the remaining 19/46 (41.3%) had abnormal results. Fifty-two (52) counts of bacteria categorized into 11 bacteria species were isolated from the 46 positive blood cultures. Enterococcus spp and streptococcus species were the commonest gram-positive while Escherichia coli and Pseudomonas luteola were the commonest gram-negative bacteria isolates. Imipenem, amoxicillin/clavulanic acid, Vancomycin, and ofloxacin had the widest coverage of bacteria isolated from newborn with sepsis. Conclusion: Neonatal sepsis is still prevalent in our environment and compared to previous documented isolates and sensitivity pattern, the bacteria causes, and their antibiotic sensitivity patterns appears to be changing.

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Incidence, bacteriological profile and risk factor analysis of neonatal sepsis in a peri urban set up of North India

International Journal of Contemporary Pediatrics ◽

10.18203/2349-3291.ijcp20202146 ◽

2020 ◽

Vol 7 (6) ◽

pp. 1349

Author(s):

Shalini Upadhyay ◽

Anita Chakravarti ◽

Tanisha Bharara ◽

Priti Agarwal

Keyword(s):

Risk Factors ◽

Neonatal Sepsis ◽

Neonatal Intensive Care ◽

Blood Cultures ◽

North India ◽

Microbiology Laboratory ◽

Coagulase Negative Staphylococci ◽

Bacteriological Profile ◽

Increased Risk ◽

Set Up

Background: The high incidence and antimicrobial resistance among the pathogens causing neonatal sepsis is alarming. In addition to substantial immediate mortality, survivors of infections in the neonatal period are at increased risk of long-term disability. The present study was conducted to know the bacteriological profile and risk factors associated with culture proven neonatal sepsis in a peri urban population.Methods: This study was conducted over a period of 4 year (2015-2019). On clinical suspicion, blood culture specimens were sent to microbiology laboratory. The organisms isolated from blood cultures were identified and tested for antimicrobial susceptibility. As part of infection control practices, environmental samples from the neonatal intensive care units were tested.Results: Of 907 blood cultures of neonates received in the microbiology laboratory, 20.7% were culture positive. Majority of the episodes occurred at or before 72 hours of life (81.4%). 54.3% were Gram positive cocci including Coagulase negative Staphylococci, Staphylococcus aureus and Enterococcus spp. The common Gram-negative pathogens included Escherichia coli, Klebsiella spp. and Pseudomonas spp. Common risk factors involved were preterm birth, low birth weight, premature rupture of membrane, prolonged labour and iatrogenic causes.Conclusions: The early signs of sepsis are often subtle and nonspecific. Therefore, a high index of suspicion is needed for early diagnosis. Rapid, reliable detection and appropriate case management can save lives of many new-borns.

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Clinical and bacteriological profile of neonatal sepsis with emerging resistance patterns

International Journal of Contemporary Pediatrics ◽

10.18203/2349-3291.ijcp20184281 ◽

2018 ◽

Vol 5 (6) ◽

pp. 2203 ◽

Cited By ~ 1

Author(s):

Rohitashwa Rajana ◽

Dhan Raj Bagri ◽

J. N. Sharma ◽

Vijay Agrawal

Keyword(s):

Birth Weight ◽

Low Birth Weight ◽

Neonatal Sepsis ◽

Antibiotic Sensitivity ◽

Polymyxin B ◽

Laboratory Evaluation ◽

Gram Positive ◽

Gram Negative ◽

Bacteriological Profile ◽

Sensitivity Pattern

Background: The present study was designed to evaluate the clinical spectrum, bacteriological profile, antibiotic sensitivity pattern and mortality due to neonatal septicemia in neonates admitted in neonatal units attached to the SMS Medical College, Jaipur.Methods: In born and out born babies of postnatal age up to 28 days who were bacteriological proven cases of septicemia were subjected to history, clinical examination and laboratory evaluation and data were analyzed statistically.Results: Out of 150 cases 67.33% neonates were preterm and 77.33% were low birth weight neonates. Gram negative organisms were most common cause of septicemia (66.6%), Both the Gram negative and Gram positive organisms were sensitive to piperacillin-tazobactam, linezolid, fosfomycin, teicoplanin, polymyxin B, colistin, ofloxacin. Both the Gram negative and Gram positive organisms were resistant against cephalosporins, ampicillin, Amoxyclav, cotrimoxazole. Gram negative isolates were most sensitive to Polymyxin B (70%) and had the highest resistance to cefepime (36%). Gram-positive organisms were most sensitive to vancomycin (84%) and linezolid (82%). Highest resistance was noted from Amoxyclav (52%).Conclusions: Preterm (<37 week) and low birth weight (<2500gm) neonates are considered as major susceptible causes of neonatal sepsis. Proper hygiene and hand washing, early detection of sepsis and judicial use of antibiotics to prevent multidrug resistance is needful in our setup.

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