scholarly journals The role of eicosapentaenoic acid and superoxide dismutase in the development of hemic hypoxia in cytomegalovirus infection in pregnant women of the first trimester

2020 ◽  
pp. 75-79
Author(s):  
N. A. Ishutina ◽  
I. A. Andrievskaya
Keyword(s):  
Superoxide Dismutase ◽  
Eicosapentaenoic Acid ◽  
Pregnant Women ◽  
Cytomegalovirus Infection ◽  
First Trimester

scholarly journals CHANGES OF PARAMETRES OF FREE-RADICAL STATUS, OF ANTIOXIDANT PROTECTION AND MORPHOLOGIC CHANGES OF ERYTHROCYTES OF PERIPHERAL BLOOD OF PREGNANT WOMEN OF THE FIRST TRIMESTER WITH CYTOMEGALOVIRUS INFECTION

2018 ◽  
Vol 1 (68) ◽  
pp. 57-62 ◽  
Author(s):  
Наталия Ишутина ◽  
Nataliya Ishutina ◽  
Ирина Андриевская ◽  
Irina Andrievskaya
Keyword(s):  
Superoxide Dismutase ◽  
Free Radical ◽  
Pregnant Women ◽  
Cytomegalovirus Infection ◽  
Thiobarbituric Acid ◽  
First Trimester ◽  
Antioxidant Protection ◽  
Diene Conjugates ◽  
First Trimester Of Pregnancy ◽  
Morphologic Changes

The aim is to study the features of changes of free-radical status, antioxidant protection and morphologic changes of erythrocytes of the peripheral blood of pregnant women of the first trimester with exacerbation of cytomegalovirus infection (CMVI). 35 pregnant women with exacerbation of CMVI in the first trimester of gestation (the main group) and 35 pregnant women without this pathology of the same age and term as the patients of the main group were examined. Erythrocytes of the venous blood stabilized by heparine as well as blood plasma became the material of the study. There has been studied the condition of lipid peroxidation (LP) by the contents of diene conjugates and active products reacting with thiobarbituric acid; the state of nonenzymatic antioxidant protection by the contents of α-tocopherol as well as of the enzyme of erythrocytes (superoxide dismutase); morphologic changes of erythrocytes. It was found out that the exacerbation of CMVI in the first trimester of pregnancy is associated with activation of LP processes which include modification of not only primary, reverse stages of this process – the increase of the concentration of diene conjugates (p<0.001), but they lead to an increased formation of secondary active products reacting with thiobarbituric acid (p<0.001), intensifying the destructive action on the membrane of erythrocytes. Primary and secondary products of LP against the decrease of concentration of erythrocyte superoxide dismutase (p<0.001) and α-tocopherol (p<0.001) are accumulated in increasing concentrations and become potential factors of erythrocytes damage causing their functional and metabolic inferiority. Metabolic disturbances and damages of cellular membranes lead to the increase of population of echinocytes, target-like and degenerative forms of erythrocytes, which can be accompanied by a high probability of intravascular hemolysis and contribute to the development of hemic hypoxia complicating the infectious process and the course of pregnancy. Within the present hyperoxidation and suppression of antioxidant protection that lead to the worsening of the rheology of blood, women with exacerbation of CMVI in the first trimester of pregnancy should be prescribed the medicine stabilizing the lipid exchange.

scholarly journals The role of linoleic acid in oxidative damage of hemoglobin due to cytomegelavirus infection during pregnancy

2019 ◽  
Vol 18 (3) ◽  
pp. 15-20
Author(s):  
I. A. Andrievskaya ◽  
N. A. Ishutina
Keyword(s):  
Hydrogen Peroxide ◽  
Superoxide Dismutase ◽  
Linoleic Acid ◽  
Glutathione Peroxidase ◽  
Oxidative Damage ◽  
Pregnant Women ◽  
Cytomegalovirus Infection ◽  
First Trimester ◽  
Oxidative Modification ◽  
Control Group

The goal is to study the mechanisms of linoleic acid-dependent oxidative damage to hemoglobin in blood erythrocytes of pregnant women with cytomegalovirus (CMV) infection during the first trimester.Materials and methods. The study included 55 CMV-seropositive pregnant women at 8–11 weeks pregnant, aged 24,7 ± 0,18 years. 20 women had exacerbated cytomegalovirus infection and in 35 women a latent course of the disease was observed. The control group consisted of 20 CMV-seronegative pregnant women, comparable in age and gestational age with the group of pregnant women with cytomegalovirus infection. The levels of linoleic acid, superoxide dismutase, glutathione peroxidase and methemoglobin in the blood were studied by the spectrophotometry. Glutathione was determined by histochemical methods, the total amount of hemoglobin was measured using the automatic hematological analyzer, and hydrogen peroxide level was calculated by enzyme immunoassay.Results. In cytomegalovirus infection, an increase in the indices of exogenous hydrogen peroxide and linoleic acid in blood erythrocytes of pregnant women is observed. During the period of acute disease in the first trimester of pregnancy it causes oxidative modification and a decrease in the level of not only superoxide dismutase, glutathione peroxidase and glutathione, the processes involved in the redox regulation of thiol-disulfide exchange of erythrocytes, but also hemoglobin with formation of large amount of methemoglobin, which contributes to reduction of oxygen metabolism and development of hemic hypoxia. In the latent course of the disease, the oxidative damage to hemoglobin in the erythrocytes of pregnant women is mitigated by the increase in the activity of redox enzymes, which maintains oxygen homeostasis at the level necessary for the development of pregnancy. 

scholarly journals Changes in fatty acid levels (saturated, monounsaturated and polyunsaturated) during pregnancy

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Estefania Aparicio ◽  
Carla Martín-Grau ◽  
Carmen Hernández-Martinez ◽  
Nuria Voltas ◽  
Josefa Canals ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Arachidonic Acid ◽  
Fatty Acid ◽  
Docosahexaenoic Acid ◽  
Serum Concentration ◽  
Eicosapentaenoic Acid ◽  
Pregnant Women ◽  
First Trimester ◽  
Third Trimester ◽  
Total N

Abstract Background During pregnancy a high amount of fatty acids (FA) is necessary to meet foetus demands, which vary during gestation. The present study describes the changes in maternal fatty acid concentrations during pregnancy in a sample of pregnant women. Methods This is a longitudinal study of 479 pregnant women who were monitored from the first trimester to third trimester of pregnancy. Data on maternal characteristics were recorded and a serum sample was collected in each trimester. The fatty acid profile (saturated (SFA: total, lauric acid, myristic acid, palmitic acid, stearic acid), monounsaturated (MUFA: total, palmitoleic acid, oleic acid) and polyunsaturated fatty acids (PUFA: total omega-6 (n-6), linoleic acid, dihomo-γ-linolenic acid, arachidonic acid (AA), total omega-3 (n-3), eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA)) was analysed with a gas chromatography-mass spectrometry combination. Results From the first trimester to third trimester of pregnancy, a significant increase in total SFA, total MUFA and total n-6 PUFA was found. (p < 0.001). Nevertheless, the serum concentration of arachidonic acid (AA), eicosapentaenoic acid (EPA) and total n-3 PUFA decreased during gestation (p < 0.001). A statistically non-significant result was observed for the docosahexaenoic acid (DHA) serum concentration between the first and third trimesters of pregnancy. Significant correlations were observed between each total fatty acid concentrations of the first and third trimesters. Conclusion The circulating serum concentration of SFA, MUFA and n-6 PUFA increases during pregnancy, whereas essential fatty acids such as AA and EPA decrease, and DHA remains unchanged. Further research is necessary to understand the role played by FA throughout gestation.

scholarly journals DISTURBANCE OF 20α-HYDROXYSTEROID DEHYDROGENASE ACTIVITY IN VILLOUS CHORION SYNCIOTHYTHROPHOBLAST AT REACTIVATION OF CYTOMEGALOVIRUS INFECTION DURING PREGNANCY

2017 ◽  
Vol 1 (65) ◽  
pp. 50-53
Author(s):  
Инна Довжикова ◽  
Inna Dovzhikova ◽  
Михаил Луценко ◽  
Mikhail Lutsenko ◽  
Ксения Петрова ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Dehydrogenase Activity ◽  
Cytomegalovirus Infection ◽  
Hydroxysteroid Dehydrogenase ◽  
First Trimester ◽  
Main Group ◽  
Control Group ◽  
Reaction Products ◽  
Igg Antibody ◽  
Antibody Titers

The aim of the work is to assess the effects of cytomegalovirus infection on the 20α-hydroxysteroid dehydrogenase activity in the villous chorion syncytiotrophoblast in the first trimester of pregnancy. The materials for the study included 48 villous chorions samples taken during spontaneous abortions at 8th–10th weeks of gestation from women with reactivation of cytomegalovirus infection (the main group). The control group included 35 villous chorions samples from pregnant women with chronic cytomegalovirus infection in the latent stage taken at medical abortions at the same stage of gestation. The results of women’s examination were studied taken into account the activity of cytomegalovirus infection by immunoassay by detection of IgM antibodies or by 4 times and more increase of IgG antibody titers in the paired serums in dynamics in 10 days. The activity of 20α-hydroxysteroid dehydrogenase was assessed by the histochemical method. Quantitative evaluation of the reaction products was carried out on sections under the microscope MT (Japan) connected to software-hardware complex «SCION Corporation» (USA). At histochemical specimen of villous chorion of pregnant women who had a reactivation of cytomegalovirus infection during pregnancy (main group), there was a decrease of cytophotometric parameter of 20α-hydroxysteroid dehydrogenase activity till 30.1±2.12 pixels/μm2 (p<0.001) compared with the control group. The decrease in the reaction activity in syncytiotrophoblast indicated a decrease in the contents of 20α-dihydroprogesterone in placenta, which contributed, in our opinion, to spontaneous abortion.

scholarly journals The Role of Routine Ultrasonography in the First Trimester of Pregnancy

2021 ◽  
pp. 950-958
Author(s):  
T. Saravanan ◽  
Hephzibah Kirubamani
Keyword(s):  
Pregnant Women ◽  
First Trimester ◽  
Antenatal Clinic ◽  
Specific Method ◽  
Medical College ◽  
Early Pregnancy Failure ◽  
Ultrasound Findings ◽  
History Of ◽  
First Trimester Of Pregnancy

Introduction: In the early stages of pregnancy, ultrasound is an extremely specific method for examination. This article reviews how the normal development of a baby in the first trimester of pregnancy relates to ultrasound findings. Aim: To highlight the importance of routine ultrasonography in the first trimester of pregnancy in detecting and dating gestation, its viability and early detection of anomalies and complications Methods: This descriptive study involved 100 pregnant women within 12 weeks of gestation attending antenatal clinic at Saveetha Medical college, during the study period Inclusion Criteria: Pregnant women with history of amenorrhea <12 weeks of gestation. Exclusion criteria: Individuals with history of pain abdomen and bleeding per vaginum Results: A total of 99 pregnancies were intrauterine of which 1 was anembryonic and 2 had early pregnancy failure, 15 pregnancies were redated. One ectopic and one fibroid complicating pregnancy were aslo found. Conclusion: Ultrasonography is an effective method to detect and date pregnancy, identify nonviable pregnancies, fetal abnormalities and early trimester complications. Hence it is ideal to use it routinely as screening tool during the first trimester of pregnancy.

scholarly journals The role of α-tocopherol and ɷ-3 polyunsaturated fatty acids in miscarriage at early stages of pregnancy with cytomegalovirus infection

2021 ◽  
pp. 98-104
Author(s):  
N. A. Ishutina ◽  
I. А. Andrievskaya ◽  
I. V. Dovzhikova ◽  
N. N. Dorofienko
Keyword(s):  
Peripheral Blood ◽  
Cytomegalovirus Infection ◽  
First Trimester ◽  
Main Group ◽  
Control Group ◽  
Gas Liquid Chromatography ◽  
Epa And Dha ◽  
Group A ◽  
Therapeutic Measures

Introduction. It is now generally acknowledged that cytomegalovirus infection (CMVI) is one of the main causes of miscarriage. However, the mechanism of this effect has not been sufficiently studied. At the same time, the influence of acids of the ɷ-3 family and α-tocopherol (αTP) on the placentation process through a pro-angiogenic action is shown.Aim. To study the concentration of αTP and ɷ-3 family acids in the peripheral blood and establish their role in miscarriage in CMV-seropositive women with CMVI reactivation.Materials and methods. A case-control study included 64 women in the first trimester of pregnancy (7-10 weeks), of which 36 were CMV-seropositive with CMVI reactivation (main group) and 28 were CMV-seronegative (control group). CMVI was diagnosed by the determination of class M and G antibodies by ELISA, as well as CMV DNA detected by PCR. The concentration of ɷ-3 acids of the family (eicosapentaenoic – EPA, docosahexaenoic – DHA) in blood serum was studied by capillary gas-liquid chromatography (J.P.Carreau, J.P.Dubacq). The αTP concentration was determined by the fluorometric method (L.G.Hansen, W.I.Warwich).Results. In women of the main group, a significant (p<0.001) decrease in the concentration of αTP to 1.32±0.025 μg/mL was observed in the peripheral blood compared to the same indicator in the control group (1.49±0.029 μg/mL). At the same time, the levels of EPA and DHA were also statistically significant (p<0.001) lower than the same indicator in the control group and amounted to 1.09±0.012 and 6.09±0.015%, respectively (in the control, 1.29±0.071 and 8.80±0.071%, respectively). Conclusion. The obtained results of the study allow us to establish the important role of disorders in the content of α-TF, EPA and DHA in the pathogenesis of miscarriage during reactivation of CMVI in the early periods of gestation, which can serve as a basis for expanding diagnostic and therapeutic measures in this pathology of pregnant women.

RELATIONSHIP OF INTERLEUKIN-6 AND BLOOD FLOW IN THE UMBILICAL ARTERY DURING EXCERVATION OF MONO- AND MIXT-CYTOMEGALOVIRAL INFECTION IN THE SECOND TRIMESTER OF PREGNANCY

2020 ◽  
Author(s):  
Irina Andrievskaya ◽  
A Milovanov ◽  
Igor Gorikov ◽  
Inna Dovzhikova ◽  
Nataliya Ishutina
Keyword(s):  
Blood Flow ◽  
Pregnant Women ◽  
Blood Supply ◽  
Interleukin 6 ◽  
Cytomegalovirus Infection ◽  
Umbilical Artery ◽  
Second Trimester ◽  
Relationship Of ◽  
The Relationship

In order to determine the role of a cytokine in the regulation of blood supply to the placenta, the relationship between interleukin-6 (IL-6) in serum and blood flow in the umbilical artery in healthy pregnant women and during pregnancy complicated by exacerbation of mono- and mixed cytomegalovirus infection in the second trimester was studied

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