scholarly journals Response of Three Sweet Maize Varieties to Leaf Blight (Exserohilum turcicum) Planted in Freshwater Swamps of South Sumatra

2021 ◽  
Vol 10 (2) ◽  
pp. 225-232
Author(s):  
Rafika Oktarida ◽  
Harman Hamidson
Keyword(s):  
Disease Severity ◽  
Control Method ◽  
Leaf Blight ◽  
Effective Control ◽  
Exserohilum Turcicum ◽  
Pests And Diseases ◽  
High Production Rate ◽  
Resistant Varieties ◽  
Maize Varieties ◽  
High Production

Planting resistant varieties is the most effective control method and is recommended because it is safe for the environment. The problem to be raised in this study was the use of the same variety of maize in every growing season by farmers. The use of one of these varieties could cause a decrease in yield due to the resistance of the variety to certain pests and diseases.  This study aimed to provide information in the form of the use of sweet maize varieties resistant to leaf blight and potential to be planted in the swampy lowland of South Sumatra. The varieties used in this study were Bonanza F1, MB-01 Sweet and Love Sweet varieties. Based on the results of the study, as a whole the use of several varieties had a significant effect on the severity of leaf blight. The results of this study showed that there were differences in the response to resistance of each variety to leaf blight caused by the fungus Exserohilum turcicum. The observation of disease severity was carried out only in the vegetative phase. The highest disease severity was found in the Bonanza, Love Sweet, and MB-01 Sweet varieties at 16.90%, 9.99% and 7.07% respectively. The production data showed that there was no significant effect on the leaf blight. The MB-01 Sweet variety had the potential to be planted on swampy soil because it had resistance to blight and had a high production rate.

Screening for resistance of grape varieties to powdery mildew (Erysiphenecator) disease

2015 ◽  
Vol 5 (1) ◽  
pp. 585-590
Author(s):  
Andekelile Mwamahonje ◽  
Deusdedit Kilambo ◽  
Leon Mrosso ◽  
Tileye Feyissa
Keyword(s):  
Powdery Mildew ◽  
Disease Severity ◽  
Control Method ◽  
Cost Effective ◽  
Spore Suspension ◽  
Effective Control ◽  
Grape Vine ◽  
Resistant Varieties ◽  
Significant Difference ◽  
High Level

This study was conducted to evaluate the susceptibility of grapevine varieties to powdery mildew. Powdery mildew is a disease caused by a fungal, Erysiphenecator, and an obligate parasite of grapevine (Vitisvinifera L.). Powdery mildew causes drastic yield losses of 50 to 70%. Commercial grapevines grown in producing countries are susceptible to powdery mildew. Use of fungicides to control the disease is expensive and not environmentally friendly. Therefore, use of grapevine resistant varieties to powdery mildew is cost-effective control method. In this study, ten varieties (Black rose, Regina, Queen of Vineyards, Alphoncelavallee, Makutupora red, Chancellor, Halilibelyji, Syrah, Ruby seedless and Makutupora white) were screened for resistance to powdery mildew, using artificial inoculation of spore suspension and dry inoculums. Infected grape leaves were sampled from the field and grounded to obtain powder which was used as dry inoculum. The spore suspension inoculum was made by mixing powder with sterilized distilled water. The inoculation was done in two blocks with concentration of 2x105 spore/ml. Disease severity was evaluated based on a scale of 0 5; 0, means immune and 5, high level of disease severity. Results showed significant difference (P<0.05) of disease among grape vine varieties evaluated. It was found that 11.1% were resistant, 33.4% tolerant and 55% susceptible to disease. Grapevine variety Chancellor showed the highest level of resistance, and Black rose the most susceptible. The study demonstrates the effectiveness of using inoculation methods in screening resistance to powdery mildew.

scholarly journals Application of exogenous salicylic acid reduces disease severity of Plasmodiophora brassicae in pakchoi (Brassica campestris ssp. chinensis Makino)

PLoS ONE ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. e0248648
Author(s):  
Dandan Xi ◽  
Xiaofeng Li ◽  
Lu Gao ◽  
Zhaohui Zhang ◽  
Yuying Zhu ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Antioxidant Enzymes ◽  
Plant Growth ◽  
Disease Severity ◽  
Control Method ◽  
Plasmodiophora Brassicae ◽  
Critical Role ◽  
Membrane Lipid ◽  
Effective Control ◽  
Ros Scavenging

Clubroot is one of the most serious diseases affecting Brassicaceae plants worldwide. However, there is no effective control method for clubroot. Salicylic acid (SA) is a plant hormone that plays a critical role in plant defense. In our study, we found the disease severity of a clubroot-sensitive cultivar of pakchoi, Xinxiaqing, was reduced with 0.6mM exogenous SA after the infection of P. brassicae. To investigate the mechanism of SA-reduced disease severity against clubroot, then we analyzed the plant growth, alteration of antioxidant enzyme system, and related gene expression of Xinxiaqing. Results showed that the clubroot incidence rate and disease index were decreased after being treated with 0.6 mM exogenous SA. Furthermore, plant growth, reactive oxygen species (ROS) contents, and membrane lipid peroxidation were changed. The activities of antioxidant enzymes, including superoxide dismutase (SOD), ascorbic acid-peroxidase (APX), catalase (CAT), and glutathione reductase (GR), were increased. Additionally, the production rates of malondialdehyde (MDA), hydrogen peroxide (H2O2), and superoxide anion (O2·–) were also inhibited. The expression levels of genes, encoding SOD, APX, CAT, and GR, were increased. By summering all results, we conclude that 0.6 mM SA contributes to the reduction of disease severity to clubroot by increasing the activities of antioxidant enzymes, abilities of osmotic regulation, and ROS scavenging to reduce the clubroot-induced damage in pakchoi.

scholarly journals Evaluation of maize genotypes for Turcicum leaf blight (Exserohilum turcicum) in Terai and inner terai of Nepal

2016 ◽  
Vol 2 (1) ◽  
pp. 109-116
Author(s):  
Tirtha Raj Rijal ◽  
Govind KC ◽  
Kesab Babu Koirala ◽  
Jiban Shrestha
Keyword(s):  
Disease Severity ◽  
Pooled Analysis ◽  
Leaf Blight ◽  
Exserohilum Turcicum ◽  
Sources Of Resistance ◽  
Maize Productivity ◽  
Maize Genotypes ◽  
Disease Severity Scale ◽  
High Yielding Varieties ◽  
Moderately Resistant

Thirty maize genotypes in 2014-2015 at Dumarwana, Nijgadh, Keureni and Rampur and ten genotypes in 2015-2016 at Anandpur, Shitalnagar, Dumarwana, Nijgadh and Rampur were evaluated for resistance to Turcicum leaf blight (Exserohilum turcicum) under farmers field conditions. The scale used for disease severity ranged from 1-5 scale based on the proportionate leaf area affected by the disease. The combined analysis over locations in 2014-2015 showed that among the 30 genotypes 25 genotypes were resistant (1.0-2.0 scale), and 5 genotypes were moderately resistant (2.1-3.0 scale). Similarly the pooled analysis over locations in 2015-2016 showed that 7 genotypes were resistant (1.0-2.0 scale) and 3 genotypes were moderately resistant (2.1-3.0 scale). The maize genotypes namely Z376-26, Z478-3, Z433-99, Z464-5, Z478-2, Z466-1, CAH1513, RML-95/RML-96, CAH1515, CAH1521, CAH1515, CAH151, CAH153, ZH114228 , Z376-9, Z466-3, Z376-5, RML-32/RML-17, RML-86/RML-96 and 900MGold were resistant with disease severity scale of 1.5 and with higher grain yield in both the years. Thus above genotypes were identified as promising sources of resistance against E. turcicum and they can be used to develop disease resistant and high yielding varieties to enhance maize productivity in terai and inner terai of Nepal.

scholarly journals Transport of Phage in Melon Plants and Inhibition of Progression of Bacterial Fruit Blotch

Viruses ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 477 ◽  
Author(s):  
Aryan Rahimi-Midani ◽  
Tae-Jin Choi
Keyword(s):  
Disease Severity ◽  
Control Method ◽  
Causal Agent ◽  
Effective Control ◽  
Bacterial Diseases ◽  
Bacterial Fruit Blotch ◽  
Acidovorax Citrulli ◽  
Alternative Means ◽  
Foliar Tissue ◽  
Important Disease

Bacterial fruit blotch (BFB) is an economically important disease in melons and watermelons for which no effective control method is available. Application of phytobacterium-infecting phage has been evaluated as an alternative means of preventing bacterial diseases in plants. Coating of seeds with bacteriophages infecting Acidovorax citrulli, the causal agent of BFB, is effective for controlling the disease, as shown in our previous study. We evaluated the transport of bacteriophage ACPWH from soil to the leaves of melon plants, and we also evaluated its effect on BFB. Leaves of melon plants were spray-inoculated with A. citrulli, and bacteriophage ACPWH was added to soil after symptoms had developed. ACPWH was detected by PCR in foliar tissue 8 h after addition to soil. DAPI-stained ACPWH accumulated at the leaf tip after 24 h. Melon treated with ACPWH showed 27% disease severity, compared to 80% for the non-treated control, indicating that ACPWH can be used to control BFB.

scholarly journals Screening of maize genotypes against southern leaf blight (Bipolaris maydis) during summer in Rampur, Chitwan, Nepal

2018 ◽  
pp. 115-122
Author(s):  
P. B. Magar ◽  
G. B. Khatri-Chhetri ◽  
S. M. Shrestha ◽  
T. R. Rijal
Keyword(s):  
Disease Severity ◽  
Block Design ◽  
Leaf Blight ◽  
Individual Plant ◽  
Bipolaris Maydis ◽  
Maize Genotypes ◽  
Resistant Varieties ◽  
Randomized Complete Block Design ◽  
Varietal Development ◽  
Southern Leaf Blight

A study was conducted during June to September, 2012, in the field at National Maize Research Program, Rampur, Chitwan, Nepal with the objective of screening 20 maize genotypes against southern leaf blight caused by Bipolaris maydis. Experiment was laid out in a randomized complete block design with three replications. Disease scoring was done as percentage of leaf area infected on individual plant at 7 days intervals starting from 50 days after sowing, for 6 times, and disease severity and mean AUDPC were calculated and yield recorded. Among the tested genotypes, there was variation in disease severity and mean AUDPC values. Disease severity was maximum on Yellow Popcorn (51.67%) and least on RML-32/RML-17 (7.27%). Based on mean AUDPC values, RML-32/RML-17 (20.53) appeared most resistant, followed by RML-4/NML-2 (23.66) and RML-4/RML-17 (25.90), while Yellow Popcorn (136.9) was found the most susceptible. Highest grain yield (4.90 t/ha) was also recorded on RML-32/RML-17 and least (2.49 t/ha) on Yellow Popcorn. Therefore the genotypes RML-32/RML-17, RML-4/NML-2 and RML-4/RML-17 could be used as the resistant varieties and source of resistance to southern leaf blight for varietal development of maize.Journal of the Institute of Agriculture and Animal Science.Vol. 33-34, 2015, Page: 115-122

scholarly journals Improvement in the Management of Leaf Blight Disease of Taro (Colocasia esculenta) with Copper and Metalaxyl Fungicides

2020 ◽  
pp. 127-136
Author(s):  
Edmund Omane ◽  
K. Asare Oduro ◽  
Eric W. Cornelius ◽  
Andrews Y. Akrofi ◽  
Eric K. Asare ◽  
...  
Keyword(s):  
Yield Loss ◽  
Control Method ◽  
Field Evaluation ◽  
Colocasia Esculenta ◽  
Leaf Blight ◽  
Cultural Control ◽  
Wet Season ◽  
Resistant Varieties ◽  
Blight Disease

Taro (Colocasia esculenta (L.) Schott) is an important staple food crop for millions of people in developing countries. Taro production is constrained by many diseases of which leaf blight, caused by Phytophthora colocasiae Raciborski, is the most destructive and it accounts for up to 80% yield loss of taro in many countries. In Ghana, the disease accounts for 25 – 50% yield loss. Non-sustainability of cultural control method and the use of resistant varieties have compelled some farmers to abandon their fields while others have converted their farms to dry season vegetable farming. In this study, copper and metalaxyl fungicides were tested for the control of leaf blight disease of taro. In vitro fungicidal activity tests and field evaluation showed that Ridomil Gold Plus 66 WP (6% metalaxyl-M + 60% copper (I) oxide) and Metalm 72 WP (12% metalaxyl + 60% copper (I) oxide) at 50 g in 15 litres of water (3.3g/L) and Nordox 75 WG (86% cuprous oxide) at 75 g in 15 litres of water (5 g/L) are effective for the control of leaf blight disease of taro at 4 weekly intervals for five consecutive months in the wet season.

EVALUATION OF NONIONIC SURFACTANTS FOR CONTROL OF CLUBROOT OF CABBAGE USING BAREROOT AND PLUG TRANSPLANTS OF TWO CULTIVARS

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 607b-607
Author(s):  
W. Tietjen ◽  
P.J. Nitzsche ◽  
W.P. Cowgill ◽  
M.H. Maletta ◽  
S.A. Johnston
Keyword(s):  
Brassica Oleracea ◽  
Disease Severity ◽  
Nonionic Surfactants ◽  
Plasmodiophora Brassicae ◽  
Severity Index ◽  
Effective Control ◽  
Disease Severity Index

`Market Prize' and `Bravo' cabbage (Brassica oleracea Var. capitata L.), transplanted as peat plug and bareroot plants into a field naturally infested with Plasmodiophora brassicae, Woronin, were treated immediately after planting with a liquid or a granular surfactant. APSA 80™, applied in transplant water, significantly reduced percent clubbing and disease severity index (DSI) compared to control treatments. Miller Soil Surfactant Granular™ did not significantly reduce percent clubbing or DSI. There was a significant effect of cultivar on percent clubbing and DSI. There was no significant effect of transplant type on percent clubbing or DSI. This year's study culminates five years of investigation of surfactants for clubroot control. Specific surfactants have proven to be an effective control of clubroot in cabbage. Chemical names used: nonylphenoxypolyethoxyethanol (APSA 80™); alpha-alkanoic-hydro omega-hydroxy poly (oxyethylene) (Miller Soil Surfactant Granular™).

scholarly journals Characterization and optimization of extracellular enzymes production by Aspergillus niger strains isolated from date by-products

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Reda Bellaouchi ◽  
Houssam Abouloifa ◽  
Yahya Rokni ◽  
Amina Hasnaoui ◽  
Nabil Ghabbour ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Culture Medium ◽  
Extracellular Enzymes ◽  
Enzyme Expression ◽  
Specific Enzyme ◽  
Enzymatic Production ◽  
High Production Rate ◽  
High Production ◽  
Incubation Temperatures ◽  
By Products

Abstract Background This work aims to study the optimal conditions of the fermentation culture medium used for the production of extracellular enzymes (amylase, cellulase, lipase, and protease) from previously isolated Aspergillus niger strains in date by-products. Results The five most powerful isolates selected based on the zone of degradation formed on Petri plates by the substrate were subjected to the quantitative evaluation of their enzymatic production. All five strains showed almost similar API-ZYM profiles, with minor variations observed at the level of some specific enzyme expression. The production of cellulase and amylase was depending on pH and incubation temperatures. ASP2 strain demonstrated the high production rate of amylase (at pH 5 and 30 °C) and cellulase (at pH 6 and 30 °C) for 96 h of incubation. Conclusion The A. niger showed the ability to produce several extracellular enzymes and can be used in the valorization of different agroindustrial residues.

scholarly journals Ginger Essential Oils-Loaded Nanoemulsions: Potential Strategy to Manage Bacterial Leaf Blight Disease and Enhanced Rice Yield

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3902
Author(s):  
Abdullahi Adamu ◽  
Khairulmazmi Ahmad ◽  
Yasmeen Siddiqui ◽  
Intan Safinar Ismail ◽  
Norhayu Asib ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Electron Microscope ◽  
Essential Oils ◽  
Disease Severity ◽  
Rice Yield ◽  
Cell Structure ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Control Treatment ◽  
Promising Alternative

The bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most serious rice diseases, causing huge yield losses worldwide. Several technologies and approaches have been opted to reduce the damage; however, these have had limited success. Recently, scientists have been focusing their efforts on developing efficient and environmentally friendly nanobactericides for controlling bacterial diseases in rice fields. In the present study, a scanning electron microscope (SEM), transmission electron microscope (TEM), and a confocal laser scanning microscope (CLSM) were utilized to investigate the mode of actions of ginger EOs on the cell structure of Xoo. The ginger EOs caused the cells to grow abnormally, resulting in an irregular form with hollow layers, whereas the dimethylsulfoxide (DMSO) treatment showed a typical rod shape for the Xoo cell. Ginger EOs restricted the growth and production of biofilms by reducing the number of biofilms generated as indicated by CLSM. Due to the instability, poor solubility, and durability of ginger EOs, a nanoemulsions approach was used, and a glasshouse trial was performed to assess their efficacy on BLB disease control. The in vitro antibacterial activity of the developed nanobactericides was promising at different concentration (50–125 µL/mL) tested. The efficacy was concentration-dependent. There was significant antibacterial activity recorded at higher concentrations. A glasshouse trial revealed that developed nanobactericides managed to suppress BLB disease severity effectively. Treatment at a concentration of 125 μL/mL was the best based on the suppression of disease severity index, AUDPC value, disease reduction (DR), and protection index (PI). Furthermore, findings on plant growth, physiological features, and yield parameters were significantly enhanced compared to the positive control treatment. In conclusion, the results indicated that ginger essential oils loaded-nanoemulsions are a promising alternative to synthetic antibiotics in suppressing Xoo growth, regulating the BLB disease, and enhancing rice yield under a glasshouse trial.

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