scholarly journals A TEN-YEAR ANALYSIS OF CHANGES IN THE SENSITIVITY OF THE LEADING UROPATHOGEN TO ANTIBACTERIAL AGENTS IN CHILDREN WITH URINARY TRACT INFECTION IN THE NEPHROLOGY DEPARTMENT

2020 ◽  
Vol 73 (7) ◽  
pp. 1360-1364
Author(s):  
Tetiana V. Budnik ◽  
Tetiana B. Bevzenko
Keyword(s):  
Relative Risk ◽  
High Sensitivity ◽  
Study Groups ◽  
E Coli ◽  
The Past ◽  
Study Results ◽  
Sensitivity Level ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

The aim: To study the prevalence of ABR among children with UTI over the past 10 years, with an assessment of the sensitivity of E. Coli to common antibiotics in dynamics. Materials and methods: The study involved 1,044 children with UTI aged from 1 month to 18 years. Examination of patients and interpretation of the results was performed in accordance with the provisions of the Declaration of Helsinki of Human Rights. According to the design, the study included 3 comparison groups: Group 1 — children of the 2009 follow-up year (n = 337), Group 2 — of 2014 (n = 328) and Group 3 — of 2019 (n = 379). Results: Escherichia coli is recognized as the leading uropathogen in all study groups: its percentage in Group 1 was 47 % (158/337), in Group 2 — 64 % (210/328) and in Group 3 — 66.5 % (252/379). The prevalence of antibacterial resistance of E. coli strains and the high dynamics of its growth are shown. So the level of resistance of E. Coli in 2019 was 70 ± 4.06 % (176/252). This was 11 % more compared to 2014 and 18.8 % more compared to 2009. The percentage of multiresistant strains tended to increase and amounted to 28 ± 9.97 % (70/252) among patients with UTI and 40 ± 9.12 % (70/176) in the structure of resistance in 2019. The relative risk of ABR increased by 1.6 times in 2019 compared to 2014 (RR2019 = 2.208 ± 0.207 [1.473;3.310], р < 0.05 vs RR2014 = 1.375 ± 0.209 [0.913;2.063]) and by 3 times compared to 2009 (RR2009 = 0.727 ± 0.209 [0.483;1.095]). Ampicillin and amoxicillin showed an equally low sensitivity level (3.5 ± 32.14 % (9/252)). Only every second child confirmed sensitivity to cefuroxime (53.6 ± 5.76 (135/252)). Ceftazidime and ciprofloxacin showed a relatively high sensitivity level — 77.4 ± 3.34 (195/252) and 83 ± 2.81 (209/252), and at the same time the rapidly growing resistance rates — almost twice as high over the past 5 years. Furazidin K showed a high sensitivity level of 85.7 ± 2.53% (216/252), the lowest level of overall resistance of 14.3 ± 15.15 % (36/252) and a slow rate of its formation. An unfavourable prognosis of an increase in the relative risk of ABR by 2.9-3.7 times in the next 5–10 years was determined among patients with UTI, provided that the existing diagnostic and treatment approaches are maintained. Conclusions: The study results are important for understanding the clinical decision on the benefits of antibacterial therapy and optimizing its empirical choice for a patient with UTI.

scholarly journals Analysis of the bactericidal effect of ozone pneumoperitoneum

2009 ◽  
Vol 24 (2) ◽  
pp. 124-127 ◽  
Author(s):  
Rodrigo Altenfelder Silva ◽  
José Eduardo Rosseto Garotti ◽  
Renata Santos Bittencourt Silva ◽  
Alessandra Navarini ◽  
Adhemar Monteiro Pacheco Jr
Keyword(s):  
Wistar Rats ◽  
Bactericidal Effect ◽  
Bactericidal Action ◽  
Co2 Pneumoperitoneum ◽  
E Coli ◽  
Carbon Dioxide Gas ◽  
Study Results ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

PURPOSE: To assess the bactericidal action of ozone pneumoperitonium, and to compare the results with CO2. METHODS: It was used 36 Wistar rats. The animals, under anesthesia, were inoculated with 2ml of E. coli ATCC at a concentration of 10(10)UFC, and 1ml of BaSO4, into the peritoneal cavity. They were divided into three groups: Group 1, CO2 pneumoperitoneum was performed for 15 minutes; Group 2, ozone pneumoperitoneum was performed for 5 minutes at a concentration of 42µg/ml, and Group 3, ozone pneumoperitoneum was performed for 5 minutes at a concentration of 62µg/ml. Six animals from each group were sacrificed after the experiment, and the remaining 6 observed for 24 hours. Material was collected from the cavity of all animals for microbiological study. RESULTS: Ozone presented a greater bactericidal effect than CO2 in those animals sacrificed immediately after pneumoperitoneum. In the animals studied 24 hours after pneumoperitoneum evidenced no difference in bactericidal effect between the two gases. Moreover, no difference in mortality was observed. CONCLUSION: Ozone has a more potent bactericidal effect than carbon dioxide gas, although this did not influence survival of the animals.

Patient Attitudes Regarding Participation in Studies of Antimicrobial Resistance

2008 ◽  
Vol 29 (2) ◽  
pp. 155-159
Author(s):  
Pam Tolomeo ◽  
Mary Wheeler ◽  
Joshua P. Metlay ◽  
Katrina Armstrong ◽  
Neil O. Fishman ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Fisher Exact Test ◽  
Swab Sample ◽  
Cross Sectional Survey ◽  
Response Options ◽  
E Coli ◽  
Study Results ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Background.A number of recent studies of antimicrobial resistance have focused on the role of antimicrobial-resistant pathogens that colonize the gastrointestinal tract. However, participation rates have been low in studies that involve fecal sampling. Attitudes toward such studies among potential study participants have not been assessed.Methods.We conducted a cross-sectional survey, enrolling 3 groups of inpatients from a large academic center. Group 1 consisted of patients who had previously participated in a cohort study of fluoroquinolone-resistant Escherichia coli, which involved the collection of perirectal swab samples. Group 2 consisted of patients who had previously refused to participate in the study of fluoroquinolone-resistant E. coli. Group 3 consisted of patients who had never been asked to participate in the study of the fluoroquinolone-resistant E. coli. The survey assessed patients' attitudes and beliefs regarding medical research and their willingness to consent to collection of a perirectal swab sample. Response options were recorded on a 5-point Likert scale. The Fisher exact test was used to compare dichotomized responses across study groups.Results.A total of 90 patients were surveyed: there were 29 in group 1 and in group 2 and 32 in group 3. Of 90 patients, 31 (35%) believed researchers might run additional tests on collected samples without informing the patient, whereas 25 (27%) believed persons other than the research team might gain access to study results. The belief that a person could get sicker as a result of a having a perirectal swab sample collected was significantly more common among patients who had previously refused to participate in the fluoroquinolone-resistant E. coli study.Conclusion.This study highlights important beliefs and attitudes that are associated with the likelihood of participating in studies of antimicrobial resistance. Explicitly addressing these concerns with eligible patients is critical to optimize participation in future studies.

scholarly journals Evaluation of Gingival Microleakage in Class II Composite Restorations with Different Lining Techniques: An In Vitro Study

Scientifica ◽  
2015 ◽  
Vol 2015 ◽  
pp. 1-6
Author(s):  
Vedavathi Bore Gowda ◽  
B. V. Sreenivasa Murthy ◽  
Swaroop Hegde ◽  
Swapna Devarasanahalli Venkataramanaswamy ◽  
Veena Suresh Pai ◽  
...  
Keyword(s):  
Study Groups ◽  
Class Ii ◽  
Composite Liner ◽  
Flowable Composite ◽  
Composite Restorations ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Aim. To compare the microleakage in class II composite restorations without a liner/with resin modified glass ionomer and flowable composite liner.Method. Forty standardized MO cavities were prepared on human permanent mandibular molars extracted for periodontal reasons and then divided into 4 groups of ten specimens. The cavity preparations were etched, rinsed, blot dried, and light cured and Adper Single Bond 2 is applied. Group 1 is restored with Filtek P60 packable composite in 2 mm oblique increments. Group 2 is precure group where 1 mm Filtek Z350 flowable liner is applied and light cured for 20 sec. Group 3 is the same as Group 2, but the liner was cocured with packable composite. In Group 4, 1 mm RMGIC, Fuji Lining LC is applied and cured for 20 sec. All the teeth were restored as in Group 1. The specimens were coated with nail varnish leaving 1 mm around the restoration, subjected to thermocycling, basic fuchsin dye penetration, sectioned mesiodistally, and observed under a stereomicroscope.Results. The mean leakage scores of the individual study groups were Group 1 (33.40), Group 2 (7.85), Group 3 (16.40), and Group 4 (24.35). Group 1 without a liner showed maximum leakage. Flowable composite liner precured was the best.

Association of Enterohemorrhagic Escherichia coliHemolysin with Serotypes of Shiga-Like-Toxin-ProducingEscherichia coli of Human and Bovine Origins

1998 ◽  
Vol 64 (11) ◽  
pp. 4134-4141 ◽  
Author(s):  
Carlton Gyles ◽  
Roger Johnson ◽  
Anli Gao ◽  
Kim Ziebell ◽  
Denis Pierard ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Severe Disease ◽  
Sheep Erythrocyte ◽  
Pcr Amplification ◽  
E Coli ◽  
Hemolysin Gene ◽  
Group 3 ◽  
Group 2 ◽  
Bovine Feces ◽  
Group 1

ABSTRACT In this study we investigated whether the enterohemorrhagicEscherichia coli (EHEC) hemolysin gene ehxAcould be used as an indicator of pathogenicity in Shiga-like-toxin-producing Escherichia coli (SLTEC) isolates. The isolates in a collection of 770 SLTEC strains of human and bovine origins were assigned to group 1 (230 human and 138 bovine SLTEC isolates belonging to serotypes frequently implicated in human disease), group 2 (85 human and 183 bovine isolates belonging to serotypes less frequently implicated in disease), and group 3 (134 bovine isolates belonging to serotypes not implicated in disease). PCR amplification was used to examine all of the SLTEC isolates for the presence of ehxA and the virulence-associated geneseae, slt-I, and slt-II. The percentages of human isolates in groups 1 and 2 that were positive forehxA were 89 and 46%, respectively, and the percentages of bovine isolates in groups 1 to 3 that were positive forehxA were 89, 51, and 52%, respectively. The percentages of human isolates in groups 1 and 2 that were positive foreae were 92 and 27%, respectively, and the percentages of bovine isolates in groups 1 to 3 that were positive for eaewere 78, 15, and 19%, respectively. The frequencies of bothehxA and eae were significantly higher for group 1 isolates than for group 2 isolates. The presence of the ehxA gene was associated with serotype, as was the presence of the eae gene. Some serotypes, such as O117:H4, lacked both eae and ehxA and have been associated with severe disease, but only infrequently. Theslt-I genes were more frequent in group 1 isolates than in group 2 isolates, and the slt-II genes were more frequent in group 2 isolates than in group 1 isolates. In a second experiment we determined the occurrence of the ehxA andslt genes in E. coli isolated from bovine feces. Fecal samples from 175 animals were streaked onto washed sheep erythrocyte agar plates. Eight E. coli-like colonies representing all of the morphological types were transferred to MacConkey agar. A total of 1,080 E. coli isolates were examined, and the ehxA gene was detected in 12 independent strains, only 3 of which were positive for slt. We concluded that the ehxA gene was less correlated with virulence than the eae gene was and that EHEC hemolysin alone has limited value for screening bovine feces for pathogenic SLTEC because of presence of the ehxA gene in bovine isolates that are not SLTEC.

A numerical analysis of major groupings in Medicago employing traditionally used characters

1981 ◽  
Vol 59 (9) ◽  
pp. 1553-1577 ◽  
Author(s):  
Ernest Small
Keyword(s):  
Numerical Analysis ◽  
Study Groups ◽  
Species Group ◽  
Annual Species ◽  
Agglomerative Clustering ◽  
Medicago Arborea ◽  
Group 3 ◽  
Group 2 ◽  
Numerical Examination ◽  
Group 1

Numerical taxonomic analyses (particularly agglomerative clustering and ordination) were conducted on 55 species of Medicago, using 75 mostly vegetative and fruiting characters. The material studied represents all conservatively accepted species of the genus, as well as 14 "problematical" species of which many are often considered to belong to the genus Trigonella, rather than to Medicago. Although the characters employed are substantially the same as those used by recent monographers who evaluated the genus by traditional subjective procedures, the present numerical examination suggests many relationships not previously appreciated.As a result of the analyses, it was judged appropriate to recognize 12 groupings. These are group 1: M. sativa (alfalfa) and 11 allied species; group 2: M. lupulina and M. secundiflora; group 3: eight problematical species which have troubled taxonomists with respect to whether they should be placed in Medicago or Trigonella; groups 4–8: each made up of a single divergent species, respectively: M. carstiensis, M. radiata, M. orbicularis, M. heyniana, and M. arborea; groups 9–12 four subgroupings of a larger group of 28 annual species. In the main, the composition of the seven polytypic groups is similar to those of taxa accepted by recent monographers, although some realignments of particular species are suggested by the present study. Groups 1 and 2 are much more similar to each other than indicated in recent treatments. Medicago arborea was discovered to be much more dissimilar to all other species examined than thought previously. Medicago hybrida of group 1 was found to be a "master link" between species traditionally placed in Medicago, and many placed in Trigonella (group 3).The 12 groupings in turn fall into three assemblages that could be recognized as subgenera, or even as genera. These are A: groups 1, 2, 9, 10, 11, and 12; B: groups 3–7, inclusive; and C: M. arborea. Further research is suggested before formal categorization is undertaken.

scholarly journals Effects of High Loading Dose of Clopidogrel in ST Elevated Myocardial Infarction Treated with Fibrinolytic Agent

2013 ◽  
Vol 36 (1) ◽  
pp. 18 ◽  
Author(s):  
Hatice Solmaz ◽  
Mehmet Akbulut ◽  
Hasan Korkmaz ◽  
Mustafa F Yavuzkir ◽  
Oğuz K Kaya ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
High Sensitivity ◽  
Serum Levels ◽  
Fibrinolytic Therapy ◽  
Loading Dose ◽  
Cardiology Clinic ◽  
Group 3 ◽  
Group 2 ◽  
Hs Crp ◽  
Group 1

Purpose: The purpose of the present study was to evaluate the effects of different loading doses of clopidogrel on ST segment resolution on ECG, changes in cardiac enzyme levels and serum levels of high-sensitivity C-reactive protein (Hs-CRP) in patients with ST elevated myocardial infarction (STEMI) treated with fibrinolytic therapy. Methods: Patients admitted to our cardiology clinic with a diagnosis of STEMI and treated with fibrinolytic therapy were included: Group 1 (n=58) received a 300 mg loading dose of clopidogrel, Group 2 (n=55) a 450 mg loading dose and Group 3 (n=59) a 600 mg loading dose. A 75 mg/d maintanence dose of clopidogrel was given in all groups. Results: All demographic characteristics and baseline laboratory parameters were statistically similar among three groups (p > 0.05). When ST resolution periods were compared, most patients in Group 3 had ST resolution at 30 minutes; Group 2 at 60 minutes and Group 1 at 90 minutes (p < 0.05). Peak levels of creatine kinase (CK) and CK-MB were as follows: Group 3, 8th hour, Group 1 and 2, 12th hour. Peak levels of those enzymes were significantly lower in Group 3 than in Group 1 and 2 ( < 0.05). Although basal hs-CRP levels of all groups were similar, the increase in hs-CRP levels at 48 hours was lower with higher clopidogrel loading doses (p < 0.05). Conclusion: In this study comparing three different clopidogrel loading doses, the higher doses provided earlier ECG resolution, earlier and lower peak CK and CK-MB levels and lower levels of hs-CRP.

scholarly journals Characteristics of Species Composition of Causative Agents in Infective Endocarditis

2020 ◽  
pp. 48-51
Author(s):  
H. Filonenko ◽  
A. Avetyan ◽  
D. Kramarenko ◽  
A. Salamanina ◽  
O. Guryeva ◽  
...  
Keyword(s):  
Infective Endocarditis ◽  
Species Composition ◽  
Treatment Algorithm ◽  
Study Groups ◽  
Coagulase Negative Staphylococcus ◽  
Study Results ◽  
Causative Agents ◽  
The Difference ◽  
Group 2 ◽  
Group 1

  Introduction. The frequency of infective endocarditis (IE) in children with congenital heart disease (CHD) is 15– 140 times higher than that in the overall population and varies from 2 to 18% according to different authors. The aim. To define the main groups of causative agents of infective endocarditis in children and adults in order to build up an antibiotic treatment algorithm. Materials and methods. The analysis of examination findings and treatment outcomes in 124 patients was conducted from 2014 to 2019. These patients received medical treatment at the Ukrainian Children`s Cardiac Center (UCCC), Kyiv. The patients were divided into two groups by age: group 1 included children aged from 6 days to 18 years (62 [50.0%]), group 2 comprised adults aged from 18 to 79 years (62 [50.0%]). Results and discussion. Forty-nine isolates were recovered from 124 patients during the analysis of microbiological study results. The frequency of bacterial IE causative agents was 26 (41.9%) in group 1, and 17 (27.4%) in group 2. Comparative analysis of the spectrum of IE pathogens revealed differences in the two study groups. Coagulase-negative staphylococcus was the most common causative agent in group 1 (46.7% of all isolated strains; n = 12) and in group 2 (44.4%; n = 8). Staphylococcus aureus in children was detected in 5 (8.1%) cases, whereas in adults only in 2 (11.1%) cases. Pathogens of the Enterococcus spp. family (E. faecalis) were recovered in both study groups: in 6.5% (n = 2) and 16.6% (n = 3) of the patients, respectively. Gram-negative flora was detected in 3 (9.7%) patients of group 1 and, in 2 (11.1%) patients of group 2. Fungal flora was more commonly found in children (7 [22.6%] cases represented by the Candida family), whereas in adults only 1 (5.6%) case represented by the Mucor family was revealed. Conclusions. Etiological structure of the infective endocarditis pathogens in both groups was represented mainly by gram-positive bacteria, with S. epidermidis (24.5%) being the most essential. The changes were revealed in the species composition of the pathogens in group 1 with fungal microflora dominance: Candida parapsilosis in 9.7%, C. albicans and C. famata in 6.5% of the total number of plated cultures in this group. The difference in surgical operations due to possible IE in both groups was established based on the specific features of operations determined by the age factor.

scholarly journals Influence of Interimplant Distance on Papilla Formation and Bone Resorption: A Clinical-radiographic Study in Dogs

10.1563/793.1 ◽  
2006 ◽  
Vol 32 (5) ◽  
pp. 218-227 ◽  
Author(s):  
Rafael R. de Oliveira ◽  
Arthur B. Novaes ◽  
Vula Papalexiou ◽  
Valdir A. Muglia ◽  
Mário Taba
Keyword(s):  
Contact Point ◽  
Radiographic Study ◽  
Radiographic Images ◽  
Clinical Measurement ◽  
The Past ◽  
Crestal Bone Loss ◽  
Papilla Formation ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Abstract Implant esthetics has been the focus of attention for the past decade, and one vital issue is the effect of interimplant distance on interimplant papilla formation and crestal bone loss. The aim of this study was to evaluate the effect of 1, 2, and 3 mm of interimplant distance on papilla formation and crestal resorption in submerged and nonsubmerged Ankylos implants after prosthetic restoration. Bilateral mandibular premolars of 7 dogs were extracted, and after 12 weeks each dog received 8 implants. Implants were placed so that 3 interimplant distances were created at 1 mm (group 1), 2 mm (group 2), and 3 mm (group 3). The sides and the position of the groups were randomly selected. Twelve weeks after placement, the implants received metallic prostheses that allowed 5 mm of space between the prosthetic contact point (CP) and the crestal bone (CB). After 8 weeks, the distance between the CP and the papilla (CP-P) and the gingival height at the distal proximal aspect of the prosthesis (CP-DE) was clinically measured. Radiographic images were obtained to measure the distance of the CP to the CB within the interimplant surfaces (CP-IP) and adjacent to the edentulous surfaces (CP-ED). The clinical measurement of CP-P for submerged and nonsubmerged implants was 3.57 ± 1.17 mm and 3.10 ± 0.82 mm for group 1, 3.57 ± 0.78 mm and 3.16 ± 0.87 mm for group 2, and 3.35 ± 0.55 mm and 3.07 ± 0.93 mm for group 3. The CP-DE was 3.25 ± 0.77 mm for submerged and 2.78 ± 0.64 mm for nonsubmerged implants. The CP-IP for the submerged and nonsubmerged implants was 6.91 ± 0.95 mm and 7.68 ± 2.73 mm for group 1, 7.46 ± 1.43 mm and 5.87 ± 1.71 mm for group 2, and 7.72 ± 0.81 mm and 7.59 ± 1.33 mm for group 3. The CP-ED was 6.77 ± 1.33 mm for submerged implants and 6.03 ± 1.58 mm for nonsubmerged implants. There were no statistical significant differences for any of the measured parameters. We conclude that when the distance from the CP to the CB was 5 mm, interimplant distances of 1 to 3 mm did not affect papilla formation or crestal resorption of submerged or nonsubmerged implants in the dog model.

scholarly journals Neuropeptide system parameters in acute herpes zoster

2020 ◽  
Vol 10 (2) ◽  
pp. 329-337
Author(s):  
S. V. Knysh ◽  
E. V. Markelova ◽  
A. I. Simakova ◽  
A. V. Karaulov
Keyword(s):  
Herpes Zoster ◽  
Basic Protein ◽  
Neuron Specific Enolase ◽  
Pain Syndrome ◽  
Study Groups ◽  
Control Group ◽  
Group 3 ◽  
Group 2 ◽  
Protein S100b ◽  
Group 1

The neuropeptides comprise an important part in the nervous system interacting with endocrine and immune systems. Peptide regulators are responsible for the continuity of communicating elements, which support homeostasis, however, despite abundant research examining neuropeptides, not all specific mechanisms and features of interacting proteins with cells and immune components have been uncovered. Objective: to perform a comprehensive assessment of neuropeptide system in patients with herpes zoster. Materials and methods: 106 in-hospital patients were examined diagnosed with herpes zoster within 2016–2019 period. Control group consisted of 30 healthy age- and sex-matched volunteers. Blood serum was collected after verifying diagnosis on day 1. After discharge, patients were monitored for signs of pain syndrome and overall state within 3 months. It allowed to divide patients into 3 groups retrospectively. Group 1 — patients with herpes zoster, accompanied by mild or moderate pain syndrome; group 2 — patients with herpes zoster, accompanied by severe pain; group 3 — patients with herpes zoster, complicated by postherpetic neuralgia. Level of serum protein s100B, myelin basic protein, nerve growth factor, brain-derived neurotrophic factor, neuron specific enolase was measured by using specific reagents purchased from “R&D Diagnostics Inc.” (США). Results. it was found that level of serum protein S100B in all groups was significantly increased compared to control group, showing no inter-group differences. Amount of myelin basic protein in all study groups vs. control was significantly higher. Moreover, level of these parameters in group 2 vs. group 1 and 3 was significantly elevated. In addition, level of nerve growth factor was significantly increased in group 1 vs. groups 2 and 3, whereas in group 3 it was significantly lower than in control and group 2. Brain-derived neurotrophic factor was significantly decreased in all the study groups compared to control, showing no significant intergroup differences. Level of neuron-specific enolase was significantly increased in group 3 vs. control as well as group 1 and 2. The data obtained allowed to identify two parameters for assessing a risk of postherpetic neuralgia in acute herpes zoster, as well as provided deeper insights into the pathogenesis of neuroimmune disorders accompanying herpes zoster.

scholarly journals Cryopreservation of human spermatozoa with penetrating and non-penetrating cryoprotectants

2019 ◽  
Vol 85 (4) ◽  
pp. 27-34
Author(s):  
E. Pavlovich ◽  
G. Gapon ◽  
T. Yurchuk ◽  
M. Petrushko
Keyword(s):  
Assisted Reproductive Technologies ◽  
Morphological Characteristics ◽  
Study Groups ◽  
Reproductive Technologies ◽  
High Rate ◽  
Motile Cells ◽  
Group 3 ◽  
Group 2 ◽  
Cryopreservation Method ◽  
Group 1

Сryopreserved spermatozoa are widely used in infertility treatment by assisted reproductive technologies. However, the spermatozoa survival rate remains low in patients with oligoastenoteratozoospermia. Therefore the development of effective cryopreservation methods for spermatozoa from pathospermia is relevant. The effectiveness of cryopreservation spermatozoa from oligoastenoteratozoospermia man using penetrating and non-penetrating cryoprotectants was compared. Sperm motility, viability and morphological characteristics were evaluated after cryopreservation with glycerol and polyvinylpyrrolidone. The average number of spermatozoa count in fresh ejaculate was (11.0±0.2) mln/ml. After isolation of active motile fraction the number of cells was (3.8±0.3) mln/ml and (84.3±8.4) % from them were motile (group 3). (78.8±6.6) % of spermatozoa cryopreserved with glycerol (group 1) and (41.4±8.1) % cryopreserved with polyvinylpyrrolidone (group 2) remained active motile. The spermatozoa viability after cryopreservation was (82.1±8.6) % and (89.6±8.6) % in group 1 and 2, respectively. Despite the high rate of spermatozoa survival in group 1 the number of motile cells decreased to (27.3±4.8) % after cryoprotectant removing stage. Morphological analysis revealed that the incidence of spermatozoa head abnormalities was (25.97±2.67), (19.21±2.67) and (20.57±1.19) % in group 1–3, respectively. The differences of spermatozoa midpiece and tail abnormalities in the study groups were statistically insignificant. The use of polyvinylpyrrolidone as a cryoprotectant allows preserving 90 % of survived spermatozoa from oligoastenoteratozoospermia men after freeze/thawing. The set of spermatozoa head, neck and midpiece abnormalities is significantly lower after cryopreservation with polyvinylpyrrolidone compared with routine method with glycerol. Two-stage spermatozoa cryopreservation method with polyvinylpyrrolidone is promising for assisted reproductive technologies since spermatozoa can be used immediately after warming for oocyte fertilization without cryoprotectant removing step.

Sign in / Sign up

Export Citation Format

Share Document