scholarly journals Isolasi Senyawa Flavonoid dari Tumbuhan Cocor Bebek Sebagai Sediaan Inhibitor Korosi

2019 ◽  
Vol 4 (2) ◽  
pp. 72
Author(s):  
Tri Reksa Saputra ◽  
Esti Purnamasari ◽  
Anderson Arnold Aloanis
Keyword(s):  
Ethyl Acetate ◽  
Chemical Structure ◽  
Room Temperature ◽  
Spectroscopic Analysis ◽  
Ethyl Acetate Extract ◽  
Ornamental Plant ◽  
Flavonoid Compound ◽  
Chromatographic Techniques ◽  
Nmr Data ◽  
Kalanchoe Pinnata

Various species of Kalanchoe plant has been widely used for traditional medicine and also as an ornamental plant. This research is a continuing search for secondary metabolites from Kalanchoe plants in Indonesia. The fresh leaves of Kalanchoe pinnata (6 kg) was extracted at room temperature with methanol to obtain a concentrated extract. The concentrated extract of methanol was further partitioned successively with n-hexane and ethyl acetate. Yellow solid of pure isolates from ethyl acetate extract was separated by various chromatographic techniques. The chemical structure of isolates was determined by spectroscopic analysis of UV, IR , MS, 1H-NMR, 13C-NMR data and a comparison wih those previously reported on literature and identified as a flavonoid compound 3,3’,4’,5,7 pentahydroxyiflavone also known as kuersetin which belong to the flavonol class.

scholarly journals A NEW CYTOTOXIC DOLABELLANE FROM THE INDONESIAN SOFT CORAL Anthelia sp.

2013 ◽  
Vol 13 (3) ◽  
pp. 216-220 ◽  
Author(s):  
Anggia Murni ◽  
Novriyandi Hanif ◽  
Junichi Tanaka
Keyword(s):  
Mass Spectrometry ◽  
Drug Development ◽  
Ethyl Acetate ◽  
Spectroscopic Data ◽  
Soft Coral ◽  
Ethyl Acetate Extract ◽  
2D Nmr ◽  
Cancer Drug ◽  
Nmr Data ◽  
Anti Cancer

One new dolabellane (1) and two known diterpenoids stolonidiol (2) and clavinflol B (3) have been isolated from the ethyl acetate extract of the Indonesian soft coral Anthelia sp. A new compound 1 exhibited a moderate cytotoxicity against NBT-T2 cells at 10 µg/mL, while known compounds 2 and 3 showed cytotoxicity at 1 and 0.5 µg/mL, respectively. Structure of the new compound 1 was elucidated by interpretation of NMR spectroscopic data (1D and 2D NMR data) and mass spectrometry (ESIMS data) as well as comparison with those of related ones. This finding should be useful for anti cancer drug development of the promising dolabellane-types compound.

scholarly journals Profil Fraksi Sitotoksik terhadap Sel Murine Leukemia P-388 dari Ekstrak Biji Honje (Etlingera elatior)

2017 ◽  
Vol 3 (1) ◽  
pp. 79-87
Author(s):  
Alfindah Rusanti ◽  
Dede Sukandar ◽  
Tarso Rudiana ◽  
Adawiah Adawiah
Keyword(s):  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Mtt Assay ◽  
Chemical Structure ◽  
Ethyl Acetate Extract ◽  
Artemia Salina ◽  
Cytotoxic Compounds ◽  
Murine Leukemia ◽  
Etlingera Elatior

The research characterization of cytotoxic fraction against P-388 leukemia murine cells from the extract honje (Etlingera elatior) seed have been reported. This research lead to isolated and characterization of cytotoxic compounds against P-388 leukemia murine cells from the extract E. elantior seed. The extract of E. elantior seed was maserated by methanol, n-hexane, and ethyl acetate, respectively and estimated their cytotoxic activity against P-388 leukemia murine cell with 3- (4, 5-dimetiltiazol-2-yl) -2,5-difeniltetrazolium bromide (MTT) assay guided toxicity test against of shrimp Artemia salina Leach. Brine shirmp Lethality Test (BSLT) method. The active extracts will be separated by fractionation using column chromatography, radial chromatography, and for analyzing the purity of isolate will estimate by HPLC. The chemical structure of pure isolate will be identified by spectroscopies data UV Vis, FTIR, NMR and MS. The ethyl acetate extract from honje seed have cytotoxic activity by leukemia P-388 cell  with IC50 19.21 µg/mL. The compound toxic as cytotoxicagainst P-388 leukemia murine cells is flavonoid compouds their is resveratrol, lapachol, apigenin, methylated chrysin, 6,2’-dihydroxyflavanone, 3-hydroxy-3,4’-dymethoxyflavone and 4’-hydroxy-5,7-dimethoxyflavanone.DOI: http://dx.doi.org/10.15408/jkv.v0i0.3640

scholarly journals Four Flavan-3-ol Compounds From The Stembark of Chisocheton balansae C.DC. (MELIACEAE)

Molekul ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. 9
Author(s):  
Unang Supratman ◽  
Mohamad Fajar ◽  
Supriatno Salam ◽  
Rani Maharani ◽  
Desi Harneti ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
13C Nmr ◽  
Chemical Structure ◽  
Ethyl Acetate Extract ◽  
2D Nmr ◽  
Stem Bark ◽  
Spectroscopic Methods ◽  
Endemic Plants ◽  
North Sulawesi ◽  
First Time

Chisocheton balansae C.DC., is one of the Meliaceae family plants which is the endemic plants from Soputan Mountain, North Sulawesi, Indonesia. This study was aimed to determine the chemical structure of flavan-3-ol compounds from ethyl acetate extract of C. balansae C.DC stembark. Dried powder of C. balansae C.DC stem bark was extracted consecutively with n-hexane, ethyl acetate, and methanol solvents. Four flavan-3-ol compounds, named catechin (1), epicatechin (2), epigallocatechin-3-O-gallate (3), and epicatechin-3-O-gallate (4) were successfully isolated from ethyl acetate extract. The chemical structure of these isolates was determined by spectroscopic methods (1H-NMR, 13C-NMR, DEPT, and 2D-NMR) and comparison with previous reported spectral data. These compounds are first time reported from this plant.

scholarly journals Oxandrastins: Antibacterial Meroterpenes from an Australian Mud Dauber Wasp Nest-Associated Fungus, Penicillium sp. CMB-MD14

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7144
Author(s):  
Ahmed H. Elbanna ◽  
Zeinab G. Khalil ◽  
Robert J. Capon
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Spectroscopic Analysis ◽  
Ethyl Acetate Extract ◽  
Culture Media ◽  
Rice Cultivation ◽  
Chemical Investigation ◽  
Penicillium Sp ◽  
Vancomycin Resistant Enterococci ◽  
Vancomycin Resistant

The ethyl acetate extract of an ISP-2 agar cultivation of the wasp nest-associated fungus Penicillium sp. CMB-MD14 exhibited promising antibacterial activity against vancomycin-resistant enterococci (VRE), with a bioassay guided chemical investigation yielding the new meroterpene, oxandrastin A (1), the first andrastin-like metabolite with an extra oxygenation at C-2. A culture media optimisation strategy informed a scaled-up rice cultivation that yielded 1, together with three new oxandrastins B–D (2–4), two known andrastins C (5) and F (6), and a new meroterpene of the austalide family, isoaustalide F (7). Structures of 1–7 were assigned based on detailed spectroscopic analysis and chemical interconversion. A GNPS molecular networking analysis of the rice cultivation extract detected the known austalides B (8), H (9), and H acid (10), tentatively identified based on molecular formulae and co-clustering with 7. That the anti-VRE properties of the CMB-MD14 extract were exclusively attributed to 1 (IC50 6.0 µM, MIC99 13.9 µM), highlights the importance of the 2-OAc and 3-OAc moieties to the oxandrastin anti-VRE pharmacophore.

scholarly journals Anthelmintic acetogenin from Annona squamosa L. Seeds

2008 ◽  
Vol 80 (2) ◽  
pp. 271-277 ◽  
Author(s):  
Marta M.C. Souza ◽  
Claudia M.L. Bevilaqua ◽  
Selene M. Morais ◽  
Cícero T.C. Costa ◽  
Ana R.A. Silva ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Haemonchus Contortus ◽  
Spectroscopic Analysis ◽  
Ethyl Acetate Extract ◽  
Anthelmintic Activity ◽  
Northeastern Brazil ◽  
Annona Squamosa ◽  
Egg Hatching

Annona squamosa seeds extracts showed anthelmintic activity against Haemonchus contortus, the main nematode of sheep and goat in Northeastern Brazil. A compound 1 was isolated from ethyl acetate extract and inhibited the egg hatching of H. contortus at 25 mg ml-1. The structure of 1 was determined as a C37 trihydroxy adjacent bistetrahydrofuran acetogenin based on spectroscopic analysis.

scholarly journals Cichorin A: a benzo-isochromene from Nypa fruticans endophytic fungus Pestalotiopsis sp.

2017 ◽  
Vol 63 (4) ◽  
pp. 13-17 ◽  
Author(s):  
Gideon O. Alade ◽  
Jones O. Moody ◽  
Olanrewaju R. Awotona ◽  
Daowan Lai ◽  
Saburi A. Adesanya ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Endophytic Fungus ◽  
Host Plants ◽  
Ethyl Acetate Extract ◽  
Bioactive Metabolites ◽  
Spectroscopic Methods ◽  
Chromatographic Techniques ◽  
Nypa Fruticans ◽  
Isolated Compound ◽  
First Time

Summary Introduction: Similar bioactive metabolites are obtainable from host plants as well as the endophytic fungi residing in them. Objective: The aim of the study is to isolate the major compound(s) from the endophytic fungus residing in Nypa fruticans Wurmb, Arecaceae family. Methods: Purification of the ethyl acetate extract of the isolated endophytic fungus was performed by employing different chromatographic techniques and structural elucidation of the isolated compound was carried out using UV and NMR spectroscopic methods. Results: Cichorin A was isolated from the ethyl acetate extract of the solid rice cultures of Pestalotiopsis sp., isolated from N. fruticans, collected in Nigeria. Conclusions: This compound is being isolated for the first time from a fungus; it is commonly isolated from the plant Cichorium intybus L. (Compositae).

scholarly journals THE ANTIBACTERIAL COMPOUND COLLISMICIN A DERIVED FROM MARINE Streptomyces sp Q-629K

2010 ◽  
Vol 8 (3) ◽  
pp. 426-430
Author(s):  
Tutik Murniasih ◽  
Kyoko Adachi
Keyword(s):  
Ethyl Acetate ◽  
Secondary Metabolite ◽  
13C Nmr ◽  
Active Compound ◽  
Chemical Structure ◽  
Ethyl Acetate Extract ◽  
Water System ◽  
Antimicrobial Compound ◽  
Streptomyces Sp

In our course of screening for secondary metabolite derived from marine bacterial, we isolate the antimicrobial compound collysmicin A from the ethyl acetate extract of Streptomyces sp Q-629K. Separation of this compound was carried out by silica gel open column chromatography. Purification of an active compound was done using HPLC C18 with acetonitril-water system. Determination of chemical structure was done by 1H, 13C NMR and LC-MS analysis. Collysmicin A was contained in fraction 3, fraction 7.2 and fraction 8.7. The antimicrobial assayed from purified compound Fr.8.7 gave diameter inhibition approximately 13 mm against S. aureus and 12 mm against B. subtilis .   Keywords: antimicrobial, collismycin A and marine Streptomyces sp

scholarly journals Isolation and Evaluation of Bioactive Compounds from Rheum emodi and their Anti-Inflammatory and Anticancer Properties

2021 ◽  
Vol 25 (06) ◽  
pp. 1161-1172
Author(s):  
Sang Koo Park
Keyword(s):  
Ethyl Acetate ◽  
Cell Lines ◽  
Cancer Cell ◽  
Ethyl Acetate Extract ◽  
Cancer Cell Lines ◽  
Flavonoid Compound ◽  
Dependent Manner ◽  
Tnf Α ◽  
Anti Inflammatory ◽  
Mcf 7

Rheum emodi Wall. ex Meissn is a popular medicinal herb having wide application in traditional medicine for treating of several diseases. The present study was aimed to identify and isolate phytochemicals present in ethyl acetate extract fraction of R. emodi and to evaluate the anticancer and anti-inflammatory activities of water/organic solvent fractions and isolated compounds of R. emodi rhizome extracts. Based on the structure, flavonoid compound i.e., Myricitrin (sym. Myricetin 3- rhamnoside), myricetin 3-galloylrhamnoside and myricetin were identified to be present in ethyl acetate extract. The molecular weight of compound 1 cannot be identified; while compound 5 remained unknown as there was not enough evidence to propose its structure. The isolated compounds and different solvent fractions were tested for their anticancer and antiinflammatory activities. Among Myricetins derivatives, particularly unknown compounds significantly induced the apoptosis and restrained the proliferation of cancer cell lines (A549 and MCF-7) and inhibited the LPS induced NO production (proinflammatory mediator), IL-6 activity, IL-1β and TNF-α (cytokines) activity in RAW 264.7 macrophages in a dose dependent manner and was effective even at lower concentration (50 µg/mL). Similarly, the higher concentration of aqueous and solvent fractions exhibited strong cytotoxic and anti-inflammatory activities. However, water and dichloromethane fractions were most effective in inhibiting the anticancer activities in A549 and MCF-7 cancer cell lines, respectively. At lower concentration (50 and 100 µg/mL), highest inhibition activity for NO, IL-6 and IL-1β was noted with ethyl acetate fractions and n-Hexane fractions; while water and n-Butanol (fractions) strongly inhibited the TNF-α activity at lower (100 µg/mL) and high (200 µg/mL) concentration, respectively. In conclusion, the isolated compounds from R. emodi rhizome extracts and its rhizome solvent fractions exhibit strong anti-cancer and anti-inflammatory activities and can be used to develop chemotherapeutics and anti-inflammation drugs. © 2021 Friends Science Publishers

scholarly journals BENZOPHENONE GLUCOSIDE ISOLATED FROM THE ETHYL ACETATE EXTRACT OF THE BARK OF MAHKOTA DEWA [Phaleria macrocarpa (Scheff.) Boerl.] AND ITS INHIBITORY ACTIVITY ON LEUKEMIA L1210 CELL LINE

2010 ◽  
Vol 9 (1) ◽  
pp. 142-145 ◽  
Author(s):  
Hendig Winarno ◽  
Ermin Katrin W
Keyword(s):  
Ethyl Acetate ◽  
Cell Line ◽  
Inhibitory Activity ◽  
Spectroscopic Analysis ◽  
Ethyl Acetate Extract ◽  
L1210 Cell ◽  
Activity Test ◽  
Physico Chemical ◽  
Phaleria Macrocarpa ◽  
Brown Powder

Isolation and elucidation of benzophenone glucoside from ethyl acetate extract of Phaleria macrocarpa bark and its inhibitory activity test against leukemia L1210 cell line have been done. The Phaleria macrocarpa bark were macerated using n-hexane, ethyl acetate, and ethanol, respectively. The ethyl acetate extract was then chromatographed on silica gel column and gradiently eluted by n-hexane - ethyl acetate - ethanol with the composition from 20:1:0 until 0:0:1, gave eight fractions. Separation of fraction 6 using semipreparative HPLC on reverse phase column (Capcell Pak C-18 SG120, 15 mm I.D. x 250 mm) using methanol - water (40:60, 5 mL/min) gave a brown powder, with the melting point of 182.3 ºC. Spectroscopic analysis and comparison of its physico-chemical data, this compound was clarified as 2,4'-dihydroxy-4-methoxy-benzophenone-6-O-b-D-glucopyranoside (3). Inhibitory activity of its compound against leukemia L1210 cell line showed that this compound exhibited inhibitory activity with IC50 was 5.1mg/mL.     Keywords: Phaleria macrocarpa, 2,4'-dihydroxy-4-methoxybenzophenone-6-O-b-D-glucopyranoside, cytotoxic activity, leukemia L1210

scholarly journals Stigmasterol, rengyolone, 2-phenylethyl β-D-glucopyranoside and n-tetradecyl-β-D-glucopyranoside from the flowers of Nyctanthes arbor-tristis Linn

2019 ◽  
Vol 54 (3) ◽  
pp. 275-282 ◽  
Author(s):  
MM Haque ◽  
N Sultana ◽  
SMT Abedin ◽  
SE Kabir
Keyword(s):  
Ethyl Acetate ◽  
1H Nmr ◽  
13C Nmr ◽  
Ethyl Acetate Extract ◽  
Natural Sources ◽  
Chromatographic Techniques ◽  
Phytochemical Investigation ◽  
First Time

A phytochemical investigation was conducted on the flowers of Nyctanthesarbor-tristis Linn. For isolation of compounds, the dried flower’s powder was successively extracted with n-hexane, dichloromethane, ethyl acetate and methanol. The extracts were fractionated using different chromatographic techniques and four compounds were isolated. Stigmasterol (1) from n-hexane, rengyolone (2) from dichloromethane and two other compounds namely, 2-phenylethyl β-D-glucopyranoside (3) and n-tetradecyl-β-D-glucopyranoside (4) from ethyl acetate extract, were isolated. These compounds (1-4) were characterized on the basis of IR, 1H NMR, 13C NMR, DEPT-135 NMR. Compounds 1 and 3 were isolated for the first time from this plant while compound 4 has been isolated and completely characterized from this plant as well as from the natural sources. Bangladesh J. Sci. Ind. Res.54(3), 275-282, 2019

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