Chemical and Biological Investigation of Apiculture Products from Stingless Bees Heterotrigona itama

Heterotrigona itama is one of common stingless bee species in Malaysia. Similar to sting bee, stingless bee also produce honey, propolis and beebread in their hive. Propolis and beebread of Malaysian stingless bee were less explored compared to honey. This work examine nutritional content, antioxidant activity and chemical profiling of honey, propolis and beebread produced by stingless bee species Heterotrigona itama. Nutritional contents such as moisture content, ash, protein, fat and carbohydrate were measured. Antioxidant activity was determined using DPPH method; Folin-Ciocalteau method for total phenolic content (TPC) and Aluminium Chloride method for total flavonoid content (TFC). Chemical profiling was carried out by using thin layer chromatography (TLC) in solvent system of toluene: ethyl acetate: formic acid with ratio 8 : 2 : 0.1 v/v/v. The results revealed that honey possessed the highest moisture content and total carbohydrate with values of 30.67%±1.30 and 65.88%±1.70, respectively. Meanwhile, propolis contained the highest total fat (49.2% ± 5.31) which due to its compositions of wax and resin. Total protein and ash contents were abundant in beebread with values of 30.43%±0.40 and 3.28%±0.13, respectively. The highest total phenolic (70.64%±01.81 µg/mL) and flavonoid contents (31.75%±0.05 µg/mL) were found in propolis. Propolis also possessed the highest antioxidant activity with the lowest IC50 value of 5 µg/mL as comparable to positive control, Trolox (IC50 value: 3µg/mL). These results revealed that propolis is a good source of antioxidant as compared to honey and beebread. TLC analysis revealed that propolis also contained higher chemical compositions as compared to honey and beebread. This finding shows that stingless bee products have potential in therapeutic as strong natural antioxidant (propolis) and high in their nutritional content (honey and beebread).

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UJI AKTIVITAS ANTIOKSIDAN DAN PENENTUAN TOTAL KANDUNGAN FENOLIK EKSTRAK ETANOL DAUN NANAMUHA (Bridelia monoica Merr)

PHARMACON ◽

10.35799/pha.9.2020.29268 ◽

2020 ◽

Vol 9 (2) ◽

pp. 178

Author(s):

Julia Elsa Lakoro ◽

Max R. J. Runtuwene ◽

Paulina V. Y. Yamlean

Keyword(s):

Antioxidant Activity ◽

Extraction Method ◽

Total Phenol ◽

Total Phenolic ◽

Total Phenol Content ◽

Phenol Compounds ◽

Activity Test ◽

Ic50 Value ◽

Dpph Method ◽

Ethanol Extracts

ABSTRACTNanamuha (Bridelia monoica Merr.) is a plant that was used as a cancer and tumor drug by the Sangihe Islands community. This plant contains phenol compounds, flavonoids, alkaloids and tannins. The purpose of this study was to determine the potential of nanamuha leaves that grow in the Sangihe Island to have antioxidant activity and determine the total phenolic ethanol extracts of nanamuha leaf. The extraction method used is maceration with ethanol solvent p.a. The extracts obtained was determined total phenolic and antioxidant activity test using DPPH method. Based on the results obtained, the ethanol extracts of nanamuha leaves has antioxidant activity with an IC50 value of 75,03 µg / mL and the result showed that the total phenol content the ethanol extracts of nanamuha leaves of 53,34 mg / L. Keywords : Nanamuha leaf, total phenol, antioxidant activity, DPPH ABSTRAKNanamuha (Bridelia monoica Merr.) merupakan tanaman yang dimanfaatkan sebagai obat kanker dan tumor oleh masyarakat Kepulauan Sangihe. Tanaman ini memiliki kandungan senyawa fenol, flavonoid, alkaloid dan tanin. Tujuan dari penelitian ini adalah unutuk mengetahui potensi dari daun nanamuha yang tumbuh di Kepulaun Sangihe ini memiliki aktivitas antioksidan dan menentukan total fenolik dari ekstrak etanol daun nanamuha. Metode ekstraksi yang digunakan adalah maserasi dengan pelarut etanol p.a. Ekstrak yang diperoleh ditentukan total fenolik dan uji aktivitas antioksidan dengan metode DPPH. Berdasarkan hasil yang didapatkan ekstrak etanol daun nanamuha memiliki aktivitas antioksidan dengan nilai IC50 75,03 µg/mL dan hasil penelitian menunjukan total kandungan fenolik ekstrak etanol daun nanamuha sebesar 53,34 mg/L. Kata kunci : Daun Nanamuha, Total fenol, Aktivitas antioksidan, DPPH

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STUDI OPTIMASI EKSTRAKSI KANDUNGAN SENYAWA FENOLIK TOTAL DAN UJI AKTIVITAS ANTIOKSIDAN DARI DAUN MANGGIS (Garcinia mangostana Linn.)

Jurnal Riset Kimia ◽

10.25077/jrk.v12i2.308 ◽

2019 ◽

Vol 10 (1) ◽

pp. 36

Author(s):

Emil Salim ◽

Yogi Afritunando ◽

Nindi Antika Febriana ◽

Mai Efdi

Keyword(s):

Antioxidant Activity ◽

Phenolic Compounds ◽

Total Phenolic Content ◽

Phenolic Content ◽

Total Phenolic ◽

Total Phenolic Compounds ◽

Garcinia Mangostana ◽

Activity Test ◽

Ic50 Value ◽

Dpph Method

This study aims to determine antioxidant activity and total phenolic content of mangosteen leaves in several different solvents. The antioxidant activity test of the extract was determined by the DPPH method and the total phenolic content was determined by the Folin-Ciocalteu method. The phytochemical test of mangosteen leaves showed the presence of phenolic compounds, saponins, triterpenoids and alkaloids. The extraction results increase with increasing water content in methanol, ethanol and acetone. The highest amount of extract was obtained using 50% acetone solvent (34.98 ± 0.17%). Extraction using 100% ethanol showed the highest total phenolic content (614.25 ± 5.58 mg GAE / g extract) and the lowest IC50 value (5.81 mg / L). The results of this study indicate that the use of 50% acetone solvent can optimize the amount of extract and 100% ethanol can optimize the content of total phenolic compounds and antioxidant activity of mangosteen leaves.

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Antioxidant Activity and Total Phenolic Content of Stingless Bee Propolis from Indonesia

Journal of Apicultural Science ◽

10.2478/jas-2019-0012 ◽

2019 ◽

Vol 63 (1) ◽

pp. 139-147

Author(s):

Al Mukhlas Fikri ◽

Ahmad Sulaeman ◽

Sri Anna Marliyati ◽

Mokhamad Fahrudin

Keyword(s):

Antioxidant Activity ◽

Ethanol Extract ◽

Stingless Bee ◽

Total Phenolic ◽

Ultrasound Assisted Extraction ◽

Phenolic Contents ◽

South Sulawesi ◽

Assisted Extraction ◽

Dpph Method ◽

Ethanol Extracts

AbstractThe aim of this study was to investigate antioxidant activity, total phenolic compounds and flavonoids of propolis from three provinces of Indonesia with two methods of extraction. Stingless bee propolis from Banten, South Kalimantan, and South Sulawesi was prepared through ultrasound-assisted extraction with water and ethanol solvents, separately. Antioxidant activity was measured by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Total phenolic and flavonoid contents were analyzed by Folin-Ciocalteu colorimetric and aluminum chloride (AlCl3) methods, respectively. The result showed that the antioxidant activity expressed as IC50 of Indonesian propolis ranged from 452.52 to 1027.29 mg/L. Ethanol extracts showed higher antioxidant activity than water extracts. Total phenolic and flavonoids content detected in the samples ranged from 10.00 to 28.65 mg/g GAE and 0.76 to 3.39 mg/g QE, respectively. The ethanol extract of propolis from South Kalimantan had the highest total phenolic contents, while that from South Sulawesi possessed the highest total flavonoids.

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Antioxidant activity screening of seven Indonesian herbal extract

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d210532 ◽

2020 ◽

Vol 21 (5) ◽

Cited By ~ 1

Author(s):

Johan Sukweenadhi ◽

Oeke Yunita ◽

Finna Setiawan ◽

KARTINI ◽

Maya Theresa Siagian ◽

...

Keyword(s):

Antioxidant Activity ◽

Biological Activities ◽

Ethanolic Extract ◽

Herbal Extract ◽

Apium Graveolens ◽

Total Phenolic ◽

Flavonoid Compounds ◽

Ic50 Value ◽

Dpph Method ◽

Activity Screening

Abstract. Sukweenadi J, Yunita O, Setiawan F, Kartini, Siagian MT, Danduru AP, Avanti C. 2020. Antioxidant Activity Screening of Seven Indonesian Herbal Extract. Biodiversitas 21: 2062-2067. Kumis kucing (Orthosiphon stamineus), pegagan (Centella asiatica), seledri (Apium graveolens), kunyit (Curcuma domestica), temulawak (Curcuma xanthorrhiza), tempuyung (Sonchus arvensis) and meniran (Phyllanthus niruri) are herbs that commonly used in the Indonesia folk medicine. The constituents that responsible for several important biological activities are phenolic and flavonoid compounds which also possess antioxidant activity. Antioxidant activity of those seven Indonesian herbal extracts was evaluated using DPPH, ABTS and FRAP methods. The extraction was done with the reflux method by using 80% ethanol as a solvent. The total phenol and total flavonoids from each herbal extract were measured using Folin–Ciocalteu reagent and spectrophotometry. Antioxidant activity results by DPPH method on O. stamineus, C. asiatica, A. graveolens, C. domestica, C. xanthorrhiza, S. arvensis, and P. niruri showed IC50 value at 132; ND; 2221; 361; 538; 1118; and 102 ppm, respectively. Results from ABTS method, showed IC50 value at 22; 1199; 169; 100; 82; 143; and 20 ppm respectively. While results from the FRAP method showed that the ethanolic extract of P. niruri at a concentration of 20 ppm possesses the strongest antioxidant activity (17.41 ppm AEAC/ppm extract). The content of total phenolic compounds are 22.50; 0.67; 2.16; 11.40; 7.80; 7.22; and 2.62% GAE, while the total flavonoid compounds were 19.88; 6.67; 4.06; 71.02; 34.62; 3.78; and 8.34% QE, respectively. It can be concluded that ethanolic extract of P. niruri and O. stamineus obtain the highest antioxidant activity based on DPPH, ABTS and FRAP method.

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Evaluation of in vitro Antioxidant and Cytotoxic Effect of Methanol Extract from aerial parts of Myristica beddomei King ssp. ustulata W.J. de Wilde

International Journal of Pharmaceutical Sciences and Drug Research ◽

10.25004/ijpsdr.2020.120204 ◽

2020 ◽

pp. 115-121

Author(s):

Neethu Joy ◽

Mahesh Mohan

Keyword(s):

Antioxidant Activity ◽

Anticancer Activity ◽

Cytotoxic Effect ◽

Total Phenolic ◽

Ovarian Cancer Cells ◽

Cytotoxicity Test ◽

Antioxidant Power ◽

Ic50 Value ◽

Dpph Method

Plants are traditionally used for pharmacological activities because of its ability to produce bioactive compounds. Myristica beddomei King ssp. ustulata W.J. de Wilde is an ethnomedicinal plant and it is seen in South Western Ghats of Kerala, India. The present study assessed the phenolic content, flavonoid concentration, in vitro antioxidant and cytotoxic effect of different parts of Myristica beddomei King. The total phenolic contents in the extracts ranged from 96.29 (pericarp) to 314.47 (bark) mg g-1 gallic acid equivalent. The concentration of flavonoids in different plant part extracts ranged from 1.81 to 2.76 mg g-1 equivalent to quercetin. All the parts exhibited potential antioxidant activity with an IC50 value of 2.87 to 9.67 μg ml-1 when compared to the standard ascorbic acid with an IC50 value of 2 μg ml-1 in 1,1-diphenyl-2- picryl-hidrasil (DPPH) method. Bark showed highest activity in terms of DPPH radical scavenging (IC50 value of 2.87 µg ml-1), phosphomolybdenum test (2261.33 ± 1.65 mg g-1 trolox equivalent) and ferric ion reducing antioxidant power (FRAP) (113.1 ± 0.28 µmol Fe2+ µg-1) while pericarp showed low antioxidant activity. The in vitro screening results revealed that the seeds exhibited promising anticancer activity compared to PA1 (Ovarian Cancer) cells (50 % inhibition) were observed at a concentration 100.68 µg ml-1. In cytotoxicity test L929 (Fibroblast) cell line compared to the other parts pericarp, mace and seed needed higher concentration (>240 µg ml-1) for LC50 value. It is a promising plant for further development of antioxidant agent as it got high content of phenolic compounds and potential antioxidant and anticancer activity.

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Optimization of Natural Antioxidants Extraction from Pineapple Peel and Their Stabilization by Spray Drying

Foods ◽

10.3390/foods10061255 ◽

2021 ◽

Vol 10 (6) ◽

pp. 1255

Author(s):

Sofia C. Lourenço ◽

Débora A. Campos ◽

Ricardo Gómez-García ◽

Manuela Pintado ◽

M. Conceição Oliveira ◽

...

Keyword(s):

Antioxidant Activity ◽

Antioxidant Capacity ◽

Spray Drying ◽

Total Phenolic Content ◽

Phenolic Content ◽

Food Products ◽

Total Phenolic ◽

Dry Extract ◽

Pineapple Peel ◽

Dpph Method

Pineapple peel still contains an important amount of phenolic compounds and vitamins with valuable antioxidant activity. In this way, the aim of this study was the recovery of the bioactive compounds from pineapple peel using environmentally friendly and low-cost techniques, envisaging their application in food products. From the solid-liquid extraction conditions tested, the one delivering an extract with higher total phenolic content and antioxidant capacity was a single extraction step with a solvent-pineapple peel ratio of 1:1 (w/w) for 25 min at ambient temperature, using ethanol-water (80–20%) as a solvent. The resulting extract revealed a total phenolic content value of 11.10 ± 0.01 mg gallic acid equivalent (GAE)/g dry extract, antioxidant activity of 91.79 ± 1.98 µmol Trolox/g dry extract by the DPPH method, and 174.50 ± 9.98 µmol Trolox/g dry extract by the FRAP method. The antioxidant rich extract was subjected to stabilization by the spray drying process at 150 °C of inlet air temperature using maltodextrin (5% w/w) as an encapsulating agent. The results showed that the antioxidant capacity of the encapsulated compounds was maintained after encapsulation. The loaded microparticles obtained, which consist of a bioactive powder, present a great potential to be incorporated in food products or to produce bioactive packaging systems.

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Evaluation of phenolic compounds and antioxidant activity in some edible flowers

Open Agriculture ◽

10.1515/opag-2020-0087 ◽

2020 ◽

Vol 5 (1) ◽

pp. 857-870

Author(s):

Fernando Gonçalves ◽

João Carlos Gonçalves ◽

Ana Cristina Ferrão ◽

Paula Correia ◽

Raquel P. F. Guiné

Keyword(s):

Antioxidant Activity ◽

Total Phenolics ◽

Total Phenolic ◽

Potential Health ◽

Phenolic Contents ◽

Spectrophotometric Methods ◽

Potential Source ◽

Successive Extraction ◽

Dpph Method ◽

Total Anthocyanins

AbstractRecently, edible flowers (EF) have aroused increased interest because of their aesthetic properties as well as potential health benefits related to the occurrence of some bioactive compounds. Therefore, the aim of this work was to evaluate the total phenolics, anthocyanins, flavonoids, and antioxidant activity (AOA) (following DPPH and ABTS methods) in eleven EF. The samples were subjected to three successive extraction steps using methanol, and these extracts were then analysed for the aforementioned properties using spectrophotometric methods. The obtained extracts were used for the quantification of phenolic composition and AOA. The results indicated that, among the flowers analysed in this study, red rose, pink rose, and red carnation possessed the highest total phenolic contents (27.53, 23.30, and 18.17 mg g−1 gallic acid equivalents, respectively), total anthocyanins (3.07, 1.97, and 4.47 mg g−1 catechin equivalents [CE], respectively), and AOA (12.07, 15.77, and 12.93 mg g−1 TE, respectively, as given by the DPPH method or 8.23, 9.27 and 8.00 mg g−1 TE, respectively, as given by the ABTS method). The flowers with highest flavonoids contents were red carnation, Mexican marigold, and pink rose (17.50, 16.90, and 16.57 mg g−1 CE, respectively). Cluster analysis grouped the analysed flowers into two groups, those richest in phenolics with AOA and those not so rich. Finally, some important correlations were observed between the total phenolics and the AOA. In conclusion, these flowers could represent a potential source of natural compounds with antioxidant capacity.

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Ekstraksi Protein dari Rhodophyta dan Chlorophyta Dari Perairan Pulau Pari Sebagai Alternatif Antioksidant

Jurnal Jaring SainTek ◽

10.31599/jaring-saintek.v1i1.186 ◽

2020 ◽

Vol 1 (1) ◽

pp. 24-31

Author(s):

Elvi Kustiyah ◽

Bungaran Saingin ◽

Hernowo Widodo ◽

Viriya Piti

Keyword(s):

Antioxidant Activity ◽

Low Temperature ◽

Protein Content ◽

Green Algae ◽

Red Algae ◽

Marine Resources ◽

Activity Test ◽

Ic50 Value ◽

Dpph Method ◽

Lowry Method

Indonesia has millions island and big part of Indonesia is sea that is rich in marine biological resources and has the potential to be developed and optimized. One of the abundant marine resources in Indonesia is algae. Algae are plant-like protists. Algae have several important compounds, including protein, carbohydrates, fats, minerals and other useful elements. Proteins contained in algae have the potential to be used as antioxidants. In this study, the levels of protein in red and green algae were tested by using the lowry method and testing the antioxidant activity of red and green algae extracts using the Diphenylpicrylhydrazyl (DPPH) method. Algae extraction was done by maceration, which is soaking the sample in low temperature with phosphate buffer saline (PBS) pH 7. From the extraction results it can be concluded that the red algae (Rhodophyta) has the highest protein content of 5.115 ± 0.126% and the lowest protein content in green algae (Chloropytha) as big as 1.686 ± 0.430%. And from the results of the antioxidant activity test showed that all positive algae showed antioxidant activity but the green algae (Chlorophyta) had the highest antioxidant activity of 71.5946 ± 0.01612% with IC50 value 1.6114.

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Phytochemical screening and in vitro antibacterial, antifungal, antioxidant and antitumor activities of the red propolis Alagoas

Brazilian Journal of Biology ◽

10.1590/1519-6984.182959 ◽

2019 ◽

Vol 79 (3) ◽

pp. 452-459 ◽

Cited By ~ 15

Author(s):

F. R. G. Silva ◽

T. M. S. Matias ◽

L. I. O. Souza ◽

T. J. Matos-Rocha ◽

S. A. Fonseca ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Phenolic Compounds ◽

Gallic Acid ◽

Bioactive Metabolites ◽

Diffusion Method ◽

Total Phenolic ◽

Phytochemical Screening ◽

Dpph Method

Abstract The study aimed to evaluate the antimicrobial activity, antioxidant, toxicity and phytochemical screening of the Red Propolis Alagoas. Antimicrobial activity was evaluated by disk diffusion method. Determination of antioxidant activity was performed using the DPPH assay (1.1-diphenyl-2-picrylhydrazyl), FTC (ferric thiocyanate) and determination of phenolic compounds by Follin method. Toxicity was performed by the method of Artemia salina and cytotoxicity by MTT method. The phytochemical screening for the detection of allelochemicals was performed. The ethanol extract of propolis of Alagoas showed significant results for antimicrobial activity, and inhibitory activity for Staphylococcus aureus and Candida krusei. The antioxidant activity of the FTC method was 80% to 108.3% hydrogen peroxide kidnapping, the DPPH method showed an EC50 3.97 mg/mL, the content of total phenolic compounds was determined by calibration curve gallic acid, resulting from 0.0005 mg/100 g of gallic acid equivalent. The extract was non-toxic by A. salina method. The propolis extract showed high activity with a higher percentage than 75% inhibition of tumor cells OVCAR-8, SF-295 and HCT116. Chemical constituents were observed as flavonones, xanthones, flavonols, and Chalcones Auronas, Catechins and leucoanthocyanidins. It is concluded that the extract can be tested is considered a potential source of bioactive metabolites.

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NEW FLAVONOIDS FROM THE AERIAL PARTS OF POLYGONUM EQUISETIFORME SM (POLYGONACEAE)

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i2.15593 ◽

2017 ◽

Vol 9 (2) ◽

pp. 166 ◽

Cited By ~ 2

Author(s):

Sayed A. El-toumy ◽

Joslin Y. Salib ◽

Nabila H. Shafik ◽

Asmaa S. Abd Elkarim ◽

Gihan A. Mick

Keyword(s):

Antioxidant Activity ◽

Methyl Ester ◽

Secondary Metabolites ◽

Gallic Acid ◽

Total Phenolic ◽

Flavonoid Content ◽

Isolation And Identification ◽

Aerial Parts ◽

Dpph Method ◽

Phenolic And Flavonoid

Objective: The current study was to deal the isolation and identification of secondary metabolites from Polygonum equisetiforme and evaluation of antioxidant activity of its extract.Methods: The methanol-water extract (7:3) of the air-dried aerial parts of Polygonum equisetiforme was fractionated and separated to obtain the isolated compounds by different chromatographic techniques. Structures of these compounds were elucidated by UV and 1D⁄2D H⁄ C NMR spectroscopy and compared with the literature data. The crude extract was evaluated for in vitro antioxidant activity using the 2,2 diphenyl dipicryl hydrazine (DPPH) method.Results: Ten secondary metabolites were isolated from Polygonum equisetiforme in this study. Of which three new flavonoids named as 3,5,7,2’,5’ pentahydroxyflavone 3-O-b-D-glucopyranoside (1), 3,5,7,2’,5’ pentahydroxyflavone 3-O-b-D-glucopyranoside 8 C-sulphated (2) and quercetin 3-O-β-D-glucucorinde 6''-methyl ester 8-sulphated (3) as well as quercetin 3-O-β-D-glucucorinde methyl ester (4), quercetin 3-O-β-D-glucopyranoside (5), quercetin 7-O-β-D-glucopyranoside (6), quercetin(7), myricetin (8), P-methoxy gallic acid methyl ester (9) and gallic acid (10). The antioxidant potential of P. equisetiforme extract was evaluated by investigating it's total phenolic and flavonoid content and DPPH radical scavenging activity whereby the extract showed significant antioxidant activity (IC50 = 37.45 μg/ml). The total phenolic and flavonoid content was found to be 130.79±5.502 and 45.8±1.63 μg/ml, respectively.Conclusion: Polygonum equisetiforme is a promising medicinal plant, and our study tends to support the therapeutic value of this plant as an antioxidant drug.

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