scholarly journals Differential Effect of Nobiletin and Tangeretin on the Antioxidant Activity and Levels of α-Tocopherol and Retinol in Rats

2021 ◽  
pp. 84-97
Author(s):  
Marilia Rodrigues ◽  
Danielle Gonçalves ◽  
Danielli Baeta ◽  
John Manthey ◽  
Thais Cesar
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Animal Models ◽  
Blood Serum ◽  
Redox Reaction ◽  
Differential Effect ◽  
Chromatographic Analysis ◽  
Blood Lipid ◽  
Blood Levels ◽  
Polymethoxylated Flavones

Polymethoxylated flavones (PMFs) have been associated with increased antioxidant activity in animal models. The effects of tangeretin (TAN) and nobiletin (NOB) on antioxidant activity in the blood and liver of rats were evaluated. Groups of rats were treated with 200 mg/kg bw/day of TAN or NOB, or placebo, for 15 days. Parental compounds and their metabolites were assessed in the liver by chromatographic analysis, in addition to α-tocopherol and retinol in the blood serum. Both TAN and NOB supplements were able to reduce malonaldehyde (MDA) in the rat’s blood by 22% and 18%, respectively, but only NOB increased redox reaction by 3%. Blood levels of retinol and α-tocopherol increased under TAN by 59% and 20%, respectively, but were not affected by NOB. Eight NOB metabolites were detected in the liver, but only two TAN metabolites were identified in low concentration. In conclusion, NOB improved antioxidant capacity and reduced lipid peroxidation, while increased levels of retinol and α-tocopherol after TAN supplement may have contributed to decreased blood lipid peroxidation.

scholarly journals Dynamics of blood lipid-phospholipid profile indicators in elderiy patients with stroke

2019 ◽  
Vol 3 (24) ◽  
pp. 45-48
Author(s):  
T. A. Slyusar ◽  
Yu. V. Abramenko ◽  
N. A. Yakovlev ◽  
K. V. Choroshavina
Keyword(s):  
Elderly Patients ◽  
Blood Serum ◽  
Blood Lipid ◽  
Functional Independence ◽  
Therapeutic Interventions ◽  
Control Group ◽  
Blood Levels ◽  
Strongly Correlated ◽  
Percentage Absorption ◽  
Phospholipid Profile

Objective. To study dynamics of blood lipid-phospholipid profile indicators in elderly patients with stroke for criteria optimization of differential diagnosis, treatment and prognosis.Material and methods. Sixty patients, aged from 60 to 74 years with new-onset hemispheric stroke were examined. 34 patients of them were diagnosed with ischemic stroke (IS); 26 patients had intracerebral hemorrhage (ICH). Patient’s status was assessed using the NIHSS, the Rivermead mobility index, the modified Renkin scale, the Barthel ADL index. We measured blood levels of phosphotidylinosits in patients with acute IS and ICH. The percentage absorption of lipid-phospholipid complexes was also studied in the infrared spectrum of blood serum. The control group consisted of 20 healthy individuals.Results. Dynamics of lipid-phospholipid complexes in blood serum is associated with the type of stroke. The blood levels of phosphotidylinosits in patients with acute IS and ICH were strongly correlated with degree of neurological deficit, levels of daily activity and functional independence (p < 0,05–0,01).Conclusion. The received data may be important for individualization of diagnostics and therapeutic interventions in elderly patients with acute IS and ICH.

scholarly journals AB1008 METABOLIC DISORDERS IN CHILDREN WITH SYSTEMIC LUPUS ERYTHEMATOSUS

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 1797.2-1798
Author(s):  
I. Chyzheuskaya ◽  
A. Vishnevskaya ◽  
I. Arher ◽  
T. Matsushko
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Lipid Peroxidation ◽  
Blood Serum ◽  
Lupus Erythematosus ◽  
Hdl Cholesterol ◽  
Blood Lipid ◽  
The State ◽  
Control Group ◽  
Systemic Lupus ◽  
Lipid Spectrum

Background:Systemic lupus erythematosus (SLE) is the most common pathology from the group of systemic diseases of the connective tissue. Currently, the problem of deadly complications in patients with SLE is progressively shifting towards vascular catastrophes, such as strokes and heart attacks. If a few years ago, patients mainly died at a young age from renal failure as a complication of lupus nephritis, now thromboses of various localization are in first place.Objectives:The purpose of the study was to determine the state of lipid peroxidation and antioxidant protection, the main indicators of the lipid spectrum and the state of hemostasis in children with SLE.Methods:25 children with SLE and 20 healthy children were examined in the rheumatology department of the 4th city children’s clinical hospital in Minsk. The lipid spectrum, lipid peroxidation (LPO) and serum ACL, ACW were determined in the Central Research Laboratory of the Belarusian Medical Academy of Postgraduate Education.Results:In children with SLE a significant increase in the content of total cholesterol (5.56±0.36 mmol/l) and a decrease in HDL-cholesterol levels (0.94±0.18 mmol/l) were found in comparison with the control group (3.71±0 69 mmol/L and 1.29±0.33 mmol/l, respectively).A significant (p <0.05) decrease in the concentration of ApoA (85.1 [59.8; 94.9] mg/dl), ApoE (2.1 [1.2; 3, 7] mg/dl) and an increase in ApoB (59.8 [51.9; 67.8] mg/dl) in children with SLE were found compared with the control group (127.2 [122.1; 132.3] mg/dl, 3, 2 [2.3; 5.9] mg/dl and 32.1 [19.9; 50.8] mg/dl, respectively). ApoB / ApoA> 1 was established in 7 (28%) children with SLE.The study found a significant (p <0.05) increase in the level of intermediate (DK233, DK278) and final (MDA) LPO products in the blood serum of children with SLE in comparison with the control group, which indicates the activation of LPO processes in these patients. During the correlation analysis, a positive correlation was established between the levels of DK233, DK278 in blood serum and CRP (rs= 0.87, p <0.001). When studying the main indicators of the blood lipid spectrum in children with SLE, a significant increase in the serum concentration of total lipids (p <0.01) and triglycerides (p <0.001) was revealed when compared with the control group.When determining indicators of coagulation hemostasis, in children with SLE, a predominance of hypercoagulation was detected, accompanied by a significant increase in serum fibrinogen level (5.08 ± 0.14 g/l) and an increase in platelet level (479.57 ± 8.01*109/l) in peripheral blood compared with the control group (3.24 ± 0.07 g / l and 294.23 ± 5.39*109/l, respectively). These indicators correlated with serum CRP concentration (rS = 0.62; p <0.01) and ESR level (rs= 0.73; p <0.01). A relationship was established between elevated serum levels of fibrinogen and disease activity indicators (rs=0.74; p <0.01).Conclusion:The atherogenic orientation of the blood lipid spectrum, characterized by hyperlipidemia, hypertriglyceridemia, hypercholesterolemia and dyslipoproteinemia in the form of a decrease in HDL-cholesterol and an increase in LDL-cholesterol, as well as an increase in ApoB/ApoA ratio> 1 and a decrease in ApoE, the activation of LPO processes and a significant decrease in ACW and ACL in the serum of children with SLE are cardiovascular events risk factors (pulmonary thromboembolism, myocardial infarction and brain).Acknowledgments:This study would not have been possible without the collaboration of numerous Belarusian pediatric rheumatologists, patients and their parents.Disclosure of Interests:None declared

scholarly journals Prognostic value of lipid peroxidation changes in acute poisoning with household drugs “bath salts”

2019 ◽  
Vol 100 (4) ◽  
Author(s):  
D B Sumnaya ◽  
A V Iksanov ◽  
V Yu Shkarednykh ◽  
V A Sadova ◽  
M V Kuleshova
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Blood Serum ◽  
Antioxidant System ◽  
Bath Salts ◽  
Acute Period ◽  
Clinical Hospital ◽  
Lipid Peroxidation Products ◽  
Peroxidation Processes ◽  
Biochemical Features

Aim. To study the state of lipid peroxidation processes in case of psychodisleptic poisoning of the “bath salt” type and to determine the clinical and biochemical markers of a favorable and complicated course of the acute period of poisoning. Methods. In 347 patients with acute household poisoning (“bath salts”) admitted to the toxicology department of the Chelyabinsk Regional Clinical Hospital №3, the clinical and biochemical features of the acute period of psychodisleptic poisoning (“bath salts”) were studied in a favorable and unfavorable course. Blood and urine samples were taken from all patients, which later underwent chemical and toxicological studies by gas chromatography-mass spectrometry. Also, lipid peroxidation and antioxidant activity were determined in selected blood samples. Lipid peroxidation products were detected in heptane-isopropanol extracts of biological material by spectrophotometry. The intensity of Fe2+-ascorbate-induced lipid peroxidation was determined in E.I. L'vovskaya's modification. The study of patients' samples was carried out in the biochemical laboratory of the regional clinical hospital №3, at the Department of Biochemistry of the Ural state university of physical culture and the South Ural state medical university of Chelyabinsk. Results. Patients with “bath salts” poisoning in the acute period showed an elevated content of all categories of peroxidation products in the blood serum — by 1.51–1.70 times more than in the control group of healthy people. A decrease in antioxidant activity values I and II by 1.13–1.31 times was also found. With favorable outcomes of poisoning, the level of activation of lipid peroxidation processes decreased with an adequate increase in the activity of the antioxidant system by 1.5–1.6 times. The activation of lipid peroxidation processes in the blood serum accompanied by a decrease in the activity of antioxidant system led to an unfavorable course of the disease and the development of cerebral edema in 18 patients which was fatal. Conclusion. The clinical and biochemical features of the course of the acute period of poisoning with “bath salts” revealed by us suggest adverse outcomes of the disease with a tendency to an increase in the content of serum lipid peroxidation products in the course of the disease (instead of reducing them) along with a reduction of antioxidant activity; these indicators should be compensated by the use of antioxidant therapy.

scholarly journals Antioxidant Activity of Thiosulfonate Compounds in Experiments in Vitro and in Vivo

2021 ◽  
Vol 12 (3) ◽  
pp. 3106-3116
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Body Weight ◽  
Blood Levels ◽  
Pathological Changes ◽  
Dpph Assay ◽  
The Relationship ◽  
Structure And Activity

The antioxidant activity of S-alkyl-4-aminobenzene-, S-allyl-4-aminobenzene- and 4-acetylaminobenzenethiosulfonates were determined by DPPH assay in experiments in vitro to establish the relationship between their structure and activity. Among the test compounds, the highest rates of these activities were found for S-allyl-4-aminobenzenethiosulfonate (ATS), S-ethyl-4-aminobenzene thiosulfonate (ETS), and S-allyl-acetyl-aminobenzenesulfonate (AATS). These compounds were studied in experiments in vivo. The processes of lipid peroxidation (LPO) and the state of the antioxidant system in the blood of rats under the influence of thiosulfonates were studied. The use of thiosulfonate additive to feed at a concentration of 100 mg/kg body weight of animals for 21 days did not cause pathological changes in rats' blood and positively influenced antioxidant blood levels.

scholarly journals Change of antioxidant protection indicators in patients with osteoarthritis in the course of the treatment with a combined chondroprotective drug

2018 ◽  
pp. 92-96
Author(s):  
E. Yu. Aleksenko ◽  
Yu. F. Gatiyatov
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Blood Serum ◽  
Total Cholesterol ◽  
Positive Impact ◽  
Term Therapy ◽  
Osteoarthritis Of The Knee ◽  
Low Density ◽  
Antioxidant Protection ◽  
Lipid Spectrum

Objective of the study. The study of changes in some parameters of the lipid peroxidation system - antioxidant protection, lipid spectrum in the course of the long-term therapy with the combined chondroprotective drug ARTRA in patients with knee osteoarthritis (OA) Methods. 24 patients with osteoarthritis of the knee joints with 1-3 X-ray stage of the disease (Kellgren and Lawrence, 1957) at the age of 41.8 ± 6.1 years were included in an open prospective study to evaluate the effects of CCD. The following indicators in serum and erythrocytes were determined: initial products of LPO (diene conjugates (DC), ketodienes and conjugated trienes (KD and CT)) in the heptane and isopropanol phase (the method of I.A. Volchegorsky, et al, 1989); intermediates (thiobarbituric acid test modified by L.I. Andreeva, et al, 1988); catalase activity (the method of M.A. Korolyuk et al., 1988); antioxidant activity of plasma (the method of M. Promyslov, 1990); peroxide erythrocyte resistance (G.A. Yarovaya, 1987). The quantitative determination of serum total cholesterol, low-density, very-low-density, high-density lipoprotein cholesterol, triglycerides was carried out using the ready-made CORMEY standardized sets (Germany). The patients’ condition and laboratory parameters were evaluated before the study, in 12 and 24 weeks after CCD was taken. Changes in the examined indicators were documented after a 24-week course. The inhibition of lipid peroxidation processes in the form of a decrease in the primary products and intermediates in the serum and erythrocytes was established. The study showed an increase in antioxidant protection (increased activity of blood serum catalase and erythrocytes and total antioxidant activity of blood serum). The patients’ tests showed a decrease in the concentration of total cholesterol and cholesterols of low-density lipoproteins. Conclusion. The study demonstrated the positive impact of ARTRA on the LPO-antioxidant protection system and the lipid spectrum in patients with OA following the 24-week administration. 

Antioxidant status of blood in patients with vibration disease

2019 ◽  
pp. 978-982 ◽  
Author(s):  
N. N. Malyutina ◽  
A. F. Bolotova ◽  
R. B. Eremeev ◽  
A. Zh. Gilmanov ◽  
D. Yu. Sosnin
Keyword(s):  
Antioxidant Activity ◽  
Blood Serum ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Main Group ◽  
Biologically Active ◽  
Antioxidant Systems ◽  
Control Group ◽  
Vibration Disease ◽  
Total Antioxidant

Introduction. The overwhelming number of publications contains only data on the content of individual antioxidants, but not on the overall antioxidant activity of the blood in patients with vibration disease.The aim of the study was to determine the total antioxidant activity of blood serum in patients with vibration disease.Materials and methods. Th e main group consisted of 30 people diagnosed with “Vibration disease” of 1 degree (n=21) and 2 degrees (n=9). Th e control group consisted of 30 clinically healthy men, comparable in age with the main group (p=0.66). Th e total activity of antioxidant systems of blood plasma was evaluated photometrically using the test system “Total antioxidant status-Novo” (“Vector-best”, Russia).Results. The indicator of the total antioxidant status (TAS) was 1,038±0.232 mmol/l in the examined main group, against 1,456±0.225 mmol/l in the examined control group (p<0.000001). Th e coefficient of variation (CV) in patients with vibration disease was 22.35%, 1.45 times higher than in the control group (15.45%). In the main group there was a positive correlation between age and TAS (R=0.525), in the control group there was no such relationship (R=0.095). Th e degree of decrease depended on the severity of vibration disease.Conclusions. 1. The development of vibration disease is accompanied by a decrease in the antioxidant status of blood serum. 2. Th e degree of decrease in the antioxidant status of blood serum correlates with the severity of vibration disease. 3. Reduction of TAS can serve as a pathogenetic justification of the need to include drugs and/or biologically active additives with antioxidant activity in therapy

SOME IMMUNOBIOCHEMICAL INDICES IN CARASSIUS CARASSIUS EXPOSED TO SUBLETHAL CONCENTRATIONS OF ZINK IONS

2017 ◽  
pp. 50-53
Author(s):  
N. I. Silkina ◽  
D. V. Mikryakov ◽  
V. R. Mikryakov
Keyword(s):  
Antioxidant Activity ◽  
Blood Serum ◽  
Immune Complexes ◽  
Chronic Exposure ◽  
Antimicrobial Properties ◽  
Carassius Carassius ◽  
Experimental Conditions ◽  
Sublethal Concentrations

Investigation results are reported on immunobiochemical indices in carassius carassius at a chronic exposure to sub-lethal Zink ions concentrations. In blood serum and liver, the following characteristics were investigated: antimicrobial properties, proportion of immune- deficient animals, content of non specific immune complexes, common lipids level, content of lipids peroxidation products and antioxidant activity. The relation between magnitudes of indices under investigation in fishes and the duration of their presence under experimental conditions is shown.

Lipid Profile of Pig Tissues Contrasting in Meat Production

2019 ◽  
Vol 09 ◽  
Author(s):  
Vitaly A. Bekenev ◽  
Anatoly A. Arishin ◽  
Sergei N. Mager ◽  
Izolda V. Bolshakova ◽  
Natalia L. Tretyakova ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Fatty Acids ◽  
Linoleic Acid ◽  
Live Weight ◽  
Blood Lipid ◽  
Meat Production ◽  
Thoracic Vertebrae ◽  
Organoleptic Properties ◽  
Biochemical Characteristics

Background: Studies were carried out on two breeds of pigs - Pietrain (P), and Kemerovo (K), contrasting in composition of carcasses, and their hybrids. Objective: The purpose of this work was to establish the characteristics of the composition of lipids of intramuscular fat (IMF), back fat and serum, organoleptic qualities in animals of these breeds. Methods: Animals of 10 individuals in each group were fed on a standard feed and slaughtered with a live weight of 95-100 kg. The quality of carcasses, the biochemical characteristics of blood, the composition of fatty acids and cholesterol in meat and fat, and blood lipid peroxidation were determined. Results: Thickness on the back fat (BF) at the level of 6-7 thoracic vertebrae with a live weight of 100 kg was 35.8 ± 1.65 mm in pigs of breed K, in hybrids - 27.6 ± 1.06, and in breed P - 19, 6 ± 1.43 mm. IMF in pigs of breed K was almost 4.5 times greater than that of breed P. IMF K breed contained 2 times less cholesterol than in P (2.34 vs. 4.68%). In the IMF, K was 2 times less polyunsaturated fatty acids (PUFA), than P, 7.82 and 15.22%, linoleic acid, 6.74% and 12.42% respectively (p &lt;0.005).The organoleptic properties of meat and salted back fat were significantly higher in breed K (P = 0.05). Conclusion: The data obtained allow us to establish priorities in the use of products of specific breeds of pigs, depending on medical requirements.

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