THE INDICATORS OF THE CARDIORESPIRATORY SYSTEM OF RATS UNDER THE ACTION OF ACETYLSALICYLIC ACID IN DIFFERENT CONCENTRATIONS

2020 ◽  
Vol 6(72) (1) ◽  
pp. 150-161
Author(s):  
M. Yu. Ravaeva ◽  
E. N. Chuyan ◽  
I. S. Mironyuk ◽  
I. V. Cheretaev ◽  
A. V. Pivovarchuk ◽  
...  
Keyword(s):  
Blood Flow ◽  
Acetylsalicylic Acid ◽  
Peripheral Resistance ◽  
Control Group ◽  
Cardiorespiratory System ◽  
Doppler Flowmetry ◽  
Scientific Equipment ◽  
Maximum Decrease ◽  
Reported Study ◽  
Almost All

The present study is devoted to revealing the peculiarities of the reaction of tissue microhemodynamics and cardiorespiratory system of animals the action acetylsalicylic acid in different concentrations on in rats. The study was carried out on the basis of the Center for collective use of scientific equipment «Experimental Physiology and Biophysics» of the Department of Human and Animal Physiology and Biophysics of the V. I. Vernadsky Crimean Federal University. The animals were divided into six groups of 10 animals. The first group was biological control; in the animals of this group, the microcirculation parameters were recorded by the laser Doppler flowmetry (LDF) method. The second-sixth group of animals was registered parameters of microcirculation and cardiorespiratory system. The LDF-metry was performed with the help of the laser analyzer of the blood flow «LAZMA-MC». It was researched the action of 5, 10, 40, 80, and 120 mg/kg doses acetylsalicylic acid on cardiorespiratory parameters in rats. It was found that the effect of acetylsalicylic acid in all doses significantly decreased only heart rate. The maximum decrease in this indicator was registered in animals when acetylsalicylic acid was administered at a dose of 120 mg/kg by 24.4 % (p≤0.05) relative to the values in the control group of animals. When acetylsalicylic acid was administered in isolated doses, there was a significant change in the activity of almost all components of microvascular tone regulation. These changes were most pronounced when animals were administered acetylsalicylic acid at a dose of 80 mg/kg. Thus, the action of acetylsalicylic acid it was increased the endothelium-dependent vasodilation, blood flow to the nutritive microvasculars, improvement in venular outflow and decreased in peripheral resistance. The reported study was funded by RFBR, project number 20-33-70142.

INDICATORS OF THE RAT CARDIORESPIRATORY SYSTEM UNDER THE ACTION OF ACETYLSALICYLIC ACID AND ITS COMPLEX COMPOUNDS WITH METALS

2020 ◽  
Vol 6(72) (2) ◽  
pp. 267-280
Author(s):  
E. N. Chuyan ◽  
I. S. Mironyuk ◽  
M. Yu. Ravaeva ◽  
I. V. Cheretaev ◽  
T. V. Grishina
Keyword(s):  
Blood Pressure ◽  
Heart Rate ◽  
Acetylsalicylic Acid ◽  
Biological Effect ◽  
Complex Compounds ◽  
Cardiorespiratory System ◽  
Animal Physiology ◽  
Scientific Equipment ◽  
Pharmaceutical Activity ◽  
Reported Study

The paper researches the effect of cobalt (АСCo2+) and zinc (АСZn2+), nickel (Ni2+) and manganese (Mn2+) acetylsalicylates in doses of 5 and 10 mg/kg on the characteristics of the rats’ cardiorespiratory system. The study was carried out on the basis of the Center for collective use of scientific equipment «Experimental Physiology and Biophysics» of the Department of Human and Animal Physiology and Biophysics of the V. I. Vernadsky Crimean Federal University. The research of the biological effect of the acetylsalicylic acid, АСCo2+, АСZn2+ , АСNi2+, АСMn2+was carried out during their intraperitoneal injection into rats in doses of 5 mg/kg and 10 mg/kg. Simultaneously the following characteristics of all the groups’ animals were registered: heart rate (HR), respiratory rate (RR), systolic blood pressure (SBP) and diastolic blood pressure (DBP). The pulse pressure (PP) was calculated on the basis of the SBP and DBP indices difference. BP, HR and RR of the rats were registered with the help of the system NIBP200A («BiopacSystems, Inc.», USA). The results of the current research allowed to determine that the coordinating compounds of the acetylsalicylic acid with such metals as Co, Zn, Ni2+, Mn2+ not only have more pronounced biological effect in comparison with the acetylsalicylic acid, but demonstrate new properties; the most effect on the cardiorespiratory system was achieved by АСZn2+. Thus, the results of the current research allow us to claim that the salts of the acetylsalicylates, having the metal-complexing in its molecules’ composition, have more pronounced and qualitatively new properties in comparison with the acetylsalicylic acid precursor’s properties; this makes the further search for biological and pharmaceutical activity of these coordinating compounds more perspective, as well as it makes the technology of complexingan inexpensive and highly efficient approach to the creation of new medicines. The reported study was funded by RFBR, project number 20-33-70142

scholarly journals Decreased prefrontal oxygenation elicited by stimulation of limb mechanosensitive afferents during cycling exercise

2018 ◽  
Vol 315 (2) ◽  
pp. R230-R240 ◽  
Author(s):  
Ryota Asahara ◽  
Kanji Matsukawa
Keyword(s):  
Blood Flow ◽  
Prefrontal Cortex ◽  
Minute Ventilation ◽  
Peripheral Resistance ◽  
Oxygenation Index ◽  
Tissue Blood Flow ◽  
Doppler Flowmetry ◽  
Blood Flows ◽  
Arterial Blood ◽  
Cutaneous Tissue

Our laboratory reported using near-infrared spectroscopy that feedback from limb mechanoafferents may decrease prefrontal oxygenated-hemoglobin concentration (Oxy-Hb) during the late period of voluntary and passive cycling. To test the hypothesis that the decreased Oxy-Hb of the prefrontal cortex would be augmented depending on the extent of limb mechanoafferent input, the prefrontal Oxy-Hb response was measured during motor-driven one- and two-legged passive cycling for 1 min at various revolutions of pedal movement in 19 subjects. Furthermore, we examined whether calculated tissue oxygenation index (TOI) decreased during passive cycling as the Oxy-Hb did, simultaneously assessing blood flows of extracranial cutaneous tissue and the common and internal carotid arteries (CCA and ICA) with laser and ultrasound Doppler flowmetry. Minute ventilation and cardiac output increased and peripheral resistance decreased during passive cycling, depending on both revolutions of pedal movement and number of limbs, whereas mean arterial blood pressure did not change. Passive cycling did not change end-tidal CO2, suggesting absence of a hypocapnic change. Prefrontal Oxy-Hb decreased during passive cycling, being in proportion to revolution of pedal movement but not number of cycling limbs. In addition, prefrontal TOI decreased during passive cycling as Oxy-Hb did, whereas blood flows of forehead cutaneous tissue, CCA, and ICA did not change significantly. Thus, a decrease in Oxy-Hb reflected a decrease in tissue blood flow of the intracerebral vasculature but not the extracerebral compartment. It is likely that feedback from mechanoafferents decreased regional cerebral blood flow of the prefrontal cortex in relation to the revolutions of pedal movement.

scholarly journals Hypertensive patients features of the microcirculation type after new coronavirus infection of COVID-19

2022 ◽  
Vol 20 (4) ◽  
pp. 45-51
Author(s):  
I. A. Zolotovskaya ◽  
G. R. Gimatdinova ◽  
I. L. Davydkin
Keyword(s):  
Blood Flow ◽  
Heart Disease ◽  
Family History ◽  
Adverse Events ◽  
Skin Blood Flow ◽  
Control Group ◽  
Doppler Flowmetry ◽  
Hypertensive Patients ◽  
History Of ◽  
And Control

Introduction. The COVID-19 pandemic multiplies the incidence of long-term adverse events. Family history of heart disease predicates the necessity of study hemostasis in hypertensive patients. The purpose was to study the microcirculation in hypertensive COVID-19 survivors. Materials and methods. We selected patients treated at the hospitals of Samara from January to March 2021. COVID-19 survivors (diagnosis was confirmed by polymerase chain reaction analysis) were divided into 2 groups and analysed: the group with family history of hypertension (167 patients) and control group with no relevant past medical history (68 patients). The main microcirculation characteristics were evaluated by laser Doppler flowmetry of skin blood flow. Results. The average age of COVID-19 survivors was 52.3±14.2 years, n=86 (51.4 %) – male. The majority of patients (n=61/36.5 %) were 45 to 54 years. A fifth of the patients (n=35/20.9 %) showed no complaints about the underlying disease, the clinical manifestations of the rest were general weakness, fever, shortness of breath, cough, headaches, unstable blood pressure, inflammation of the ENT organs. By comparison the microcirculation parameters of the case patients and control group, a decreased perfusion was detected. It requires a preventive medicine to prevent from early and distant micro and macrothrombosis. Conclusion. The study of microcirculation essential features by the LDF of skin blood flow revealed a pathological type with a predominance of vasoconstrictions in patients with a history of hypertension. Taking into account the proven increase in the incidence of thrombotic events in COVID-19 survivors, the noticed changes in microcirculation require a new approach to prevent from adverse events concerning the hypercoagulation process, especially in patients with famil y history of heart disease.

scholarly journals ASSESSMENT OF THE NATURE OF THE MICROCIRCULATORY RESPONSE TO EXPERIMENTAL THERMAL INJURY

2020 ◽  
pp. 123-129
Author(s):  
А.К. Martusevich ◽  
◽  
А.А. Yepishkina ◽  
L.R. Dilenyan ◽  
◽  
...  
Keyword(s):  
Blood Flow ◽  
Thermal Injury ◽  
Negative Impact ◽  
Small Diameter ◽  
Main Group ◽  
The State ◽  
Border Zone ◽  
Control Group ◽  
Thermal Burn ◽  
Doppler Flowmetry

Burn disease affects the functioning of almost all functional systems, having a negative effect on them due to the development of endotoxicosis and vascular disorders. At the same time, the greatest attention is paid to the shifts that form in the systemic hemodynamics, primarily in the coronary and pulmonary blood flow, which can cause the development and progression of life-threatening conditions. At the same time, the microcirculatory system is also undergoing changes, but these changes have been studied in much less detail. The aim of this research was to study the state of skin microcirculation in healthy and burned rats. The experiment was carried out on 20 adult male Wistar rats divided into two equal groups. The first group of animals (n=10) was a control group (intact), with its representatives there were not any manipulations, except for a single study of the state of microcirculation. Rats of the second (main) group (n=10) were simulated contact thermal burn using their own patented method. The assessment of microcirculation parameters in the main group of animals was carried out by laser Doppler flowmetry on the device "LAKK-M" on the 1st day (2-3 hours after the injury), on the third and tenth days after the burn period. It was found that thermal injury has a negative impact on the microcirculation system, which is seen in a decrease in the intensity of blood flow through small-diameter vessels. This is achieved by reflex vasospasm, reducing the release of nitrogen oxide by endothelial cells and activating precapillary sphincters, and simultaneously activating the shunting ways of cardiac shunt from the arterial directly to the venous channel, bypassing the capillaries. Thus, in the post-thermal period, a compensatory "robbing phenomenon" is realized in the border zone of the burn, which requires pathogenetic correction.

Evaluation of efficiency of complex treatment of patients with generalized chronic parodontitis and bruxism

2019 ◽  
Vol 24 (3) ◽  
pp. 254-257
Author(s):  
R. R. Khaybullina ◽  
L. P. Gerasimova ◽  
M. F. Kabirova ◽  
R. Z. Rakhmatullina ◽  
E. D. Tkachenko ◽  
...  
Keyword(s):  
Blood Flow ◽  
Chronic Periodontitis ◽  
Voluntary Contraction ◽  
Control Group ◽  
Bioelectric Activity ◽  
Temporal Muscle ◽  
Doppler Flowmetry ◽  
Flow Perfusion ◽  
Normal Functional ◽  
Baseline Values

Relevance: the purpose of this work was to study the effectiveness of proposed treatment and rehabilitation complex for patients with generalized chronic periodontitis and bruxism.Materials and methods: we examined 73 patients aged 30 to 45 years diagnosed with generalized chronic periodontitis of moderate severity and bruxism. Control group included 15 healthy individuals of the same age for clarify normal functional parameters. Clinical, Doppler flowmetry and electromyographic methods were used for evaluation of the effectiveness of proposed treatment and rehabilitation complex.Results: the course of treatment resulted in the increase in blood flow perfusion volume by 37.7% (p < 0.05), of blood flow perfusion rate by 58.2% (p<0.05) from baseline values. At the same time, the decrease in bioelectric activity of temporal muscle at rest by 51.82% (p >< 0.05), at voluntary contraction – by 40.4% (p<0.05), the decrease in bioelectric activity of masseter muscle at rest – by 47.4% (p><0.05), at voluntary contraction - by 35.12% (p >< 0.05) from values before treatment, were observed.Conclusion: data obtained on the basis of electromyography and laser Doppler flowmetry showed that developed treatment and rehabilitation complex is an effective method for correcting the microcirculation in periodontal vessels and the functional activity of the muscles of maxillofacial area.

The state of microcirculation and identification of microcirculatory predictors of chronic pain in younger patients with functional disorders of the masticatory system affected by connective tissue dysplasia

2020 ◽  
Vol 25 (4) ◽  
pp. 308-316
Author(s):  
E. Е. Statovskaya
Keyword(s):  
Chronic Pain ◽  
Blood Flow ◽  
Connective Tissue ◽  
Pain Syndrome ◽  
High Amplitude ◽  
Masticatory Muscles ◽  
Main Group ◽  
Control Group ◽  
Doppler Flowmetry ◽  
Connective Tissue Dysplasia

Relevance. Occlusal splints, made for the patients with temporomandibular disorders (TMD), influence proprioceptive sensitivity, sensory characteristics of pain, parameters of microcirculation of the pulp and periodontium of the healthy teeth. Condition of the pulp and periodontium of the healthy teeth should be assessed in patients with TMD associated with connective tissue dysplasia (CTD).Materials and methods. The study examined 36 TMD patients aged 26.3 ± 1.3 лет (М ± m) with CTD (n = 20, main group) and without CTD (n = 16, control group); with natural teeth, without complaints of TMD, masticatory muscles and periodontal condition. Laser doppler flowmetry (LDF) helped to analyze blood flow parameters in the periodontium and pulp of the healthy teeth. Received results were statistically analyzed.Results. Wavelet analysis revealed different microcirculatory flowmotion: high-amplitude irregular type was in patients of the main group, low-amplitude regular type was in controls. In the main group, perfusion is supported by high values of flux and vasomotion, ergotropic sympathetic effects (controlled by catecholamines) prevail, myogenic tone is reduced, elevated microvascular tone reflects possible changes in rheologic blood properties, microcirculatory signs of pain syndrome and relatively elevated ischemic index in pulp microcirculation of healthy teeth.Conclusion. Sensory and regulatory mechanisms, reflected by the condition of periodontium and pulp blood flow, can be regarded as early signs of inflammation and chronic pain in the diagnosis of TMD in patients without complaints.

THE ROLE OF LASER AND ULTRASONIC DOPPLER FLOWMETRY IN THE CORRECTION OF COSMETIC DEFECTS OF THE ANTERIOR ABDOMINAL WALL AND PREVENTION OF COMPLICATIONS

2021 ◽  
Vol 74 (7) ◽  
pp. 1605-1611
Author(s):  
Orest V. Panchuk ◽  
Yaroslav M. Susak ◽  
Ievgen G. Donets ◽  
Pavlo l. Byck ◽  
Olena F. Panchuk ◽  
...  
Keyword(s):  
Blood Flow ◽  
Abdominal Wall ◽  
Anterior Abdominal Wall ◽  
Main Group ◽  
Control Group ◽  
Entire Study ◽  
Doppler Flowmetry ◽  
Significant Difference ◽  
Simultaneous Operations ◽  
Ultrasonic Doppler Flowmetry

The aim: Of work is to determine changes in blood flow in the vessels of the anterior abdominal wall that occur after plastic surgeries in order to improve the results of operations and to develop new methods for the prevention of complications. Materials and methods: The study was conducted in 132 patients. Patients were divided into 2 groups: main group 64 and control group 68 patients. Main group has patients who underwent abdominoplasty in combination with liposuction; control group has patients who underwent abdominoplasty without liposuction. In both groups we make different simultaneous operations. Laser Doppler Flowmetry and Ultrasonic Doppler Flowmetry were performed to determine the blood flow indices in the flaps. Results: Liposuction volumes averaged 3.57 ± 0.74 liters of lipoaspirate. In the main group there were totaly 4 complications, in the control group complications developed in 9 patients. Comparing daily indicators between the two groups, no statistically significant difference in the dynamics of MI changes was found during the entire study period (p = 0.767). Increase in caliber of vessels, on average, from 1.55 ± 0.8 mm in the preoperative period to 1.68 ± 0.75 mm on the 14th day of the postoperative period was statistically significant (p < 0.05). Conclusions: The combination of abdominoplasty with liposuction and simultaneous operations does not lead to greater development of complications and allows to achieve good aesthetic results.

scholarly journals Hypotension during Fluid-restricted Abdominal Surgery

2011 ◽  
Vol 114 (3) ◽  
pp. 557-564 ◽  
Author(s):  
Luzius B. Hiltebrand ◽  
Eliana Koepfli ◽  
Oliver Kimberger ◽  
Gisli H. Sigurdsson ◽  
Sebastian Brandt
Keyword(s):  
Blood Flow ◽  
Abdominal Surgery ◽  
Oxygen Tension ◽  
Intestinal Tract ◽  
Tissue Oxygen Tension ◽  
Control Group ◽  
Tissue Oxygen ◽  
Microcirculatory Blood Flow ◽  
Intestinal Tissue ◽  
Doppler Flowmetry

Background Vasopressors, such as norepinephrine, are frequently used to treat perioperative hypotension. Increasing perfusion pressure with norepinephrine may increase blood flow in regions at risk. However, the resulting vasoconstriction could deteriorate microcirculatory blood flow in the intestinal tract and kidneys. This animal study was designed to investigate the effects of treating perioperative hypotension with norepinephrine during laparotomy with low fluid volume replacement. Methods Twenty anesthetized and ventilated pigs were randomly assigned to a control or treatment (norepinephrine) group. Both groups received 3 ml · kg⁻¹ · h⁻¹ Ringer's lactate solution. In addition, the norepinephrine group received norepinephrine to stepwise increase blood pressure to 65 and 75 mmHg. Regional blood flow was measured in the splanchnic arteries. In the small bowel and colon, microcirculatory blood flow was measured using laser Doppler flowmetry. Intestinal tissue oxygen tension was measured with intramural Clark-type electrodes. Results Hepatosplanchnic and kidney blood flow remained unchanged after reversal of arterial hypotension to a mean arterial pressure of 75 mmHg with norepinephrine. For the norepinephrine group versus the control group, the mean ± SD microcirculatory blood flow in the jejunum (96 ± 41% vs. 93 ± 18%) and colon (98 ± 19% vs. 97 ± 28%) and intestinal tissue oxygen tension (jejunum, 45 ± 13 vs. 43 ± 5 mmHg; colon, 50 ± 10 vs. 45 ± 8 mmHg) were comparable. Conclusions In this model of abdominal surgery in which clinical conditions were imitated as close as possible, treatment of perioperative hypotension with norepinephrine had no adverse effects on microcirculatory blood flow or tissue oxygen tension in the intestinal tract.

scholarly journals Endothelial dysfunction and the risk of atherosclerosis in overt and subclinical hypothyroidism

2018 ◽  
Vol 7 (10) ◽  
pp. 1075-1080 ◽  
Author(s):  
Aasem Saif ◽  
Shrook Mousa ◽  
Maha Assem ◽  
Nashwa Tharwat ◽  
Alaa Abdelhamid
Keyword(s):  
Blood Flow ◽  
Endothelial Function ◽  
Subclinical Hypothyroidism ◽  
Doppler Ultrasonography ◽  
Intima Media Thickness ◽  
Carotid Intima Media Thickness ◽  
Control Group ◽  
Doppler Flowmetry ◽  
Increased Risk ◽  
Age And Sex

Hypothyroidism is associated with increased risk of atherosclerosis. We assessed carotid intima-media thickness (CIMT), as a marker of atherosclerosis, and endothelial function in patients with hypothyroidism. We included 70 female patients with hypothyroidism in the study, 40 patients with overt and 30 patients with subclinical hypothyroidism. Forty, age- and sex-matched, subjects with normal thyroid functions were also included as a control group. CIMT was measured using high-resolution color-coded Doppler ultrasonography. Endothelial function was assessed by measuring the percent of change in blood flow following heat-mediated vasodilation using laser Doppler flowmetry. CIMT was significantly higher in patients with overt and subclinical hypothyroidism as compared with the control group (0.7 ± 0.2 and 0.6 ± 0.2 mm respectively vs 0.45 ± 0.07 mm, P < 0.001 for both). The percent of change in blood flow following heat-mediated vasodilation was significantly impaired in patients with overt and subclinical hypothyroidism as compared with the control group (328 ± 17 and 545 ± 406% respectively vs 898 ± 195%, P < 0.001 for both). The impairment was more significant in overt as compared with subclinical hypothyroidism (P = 0.014). CIMT negatively correlated with percent of change in blood flow following heat-mediated vasodilation in patients with overt and subclinical hypothyroidism (P < 0.001 for both). We concluded that CIMT is significantly higher in patients with overt and subclinical hypothyroidism compared with normal control subjects. Impairment of endothelial function is a contributing factor to the increased risk of atherosclerosis in both groups of patients.

scholarly journals Heat acclimation improves cutaneous vascular function and sweating in trained cyclists

2010 ◽  
Vol 109 (6) ◽  
pp. 1736-1743 ◽  
Author(s):  
Santiago Lorenzo ◽  
Christopher T. Minson
Keyword(s):  
Blood Flow ◽  
Skin Blood Flow ◽  
Vascular Function ◽  
Structural Changes ◽  
Forearm Blood Flow ◽  
Sweat Rate ◽  
Local Heating ◽  
Heat Acclimation ◽  
Control Group ◽  
Doppler Flowmetry

The aim of this study was to explore heat acclimation effects on cutaneous vascular responses and sweating to local ACh infusions and local heating. We also sought to examine whether heat acclimation altered maximal skin blood flow. ACh (1, 10, and 100 mM) was infused in 20 highly trained cyclists via microdialysis before and after a 10-day heat acclimation program [two 45-min exercise bouts at 50% maximal O2 uptake (V̇o2max) in 40°C ( n = 12)] or control conditions [two 45-min exercise bouts at 50% V̇o2max in 13°C ( n = 8)]. Skin blood flow was monitored via laser-Doppler flowmetry (LDF), and cutaneous vascular conductance (CVC) was calculated as LDF ÷ mean arterial pressure. Sweat rate was measured by resistance hygrometry. Maximal brachial artery blood flow (forearm blood flow) was obtained by heating the contralateral forearm in a water spray device and measured by Doppler ultrasound. Heat acclimation increased %CVCmax responses to 1, 10, and 100 mM ACh (43.5 ± 3.4 vs. 52.6 ± 2.6% CVCmax, 67.7 ± 3.4 vs. 78.0 ± 3.0% CVCmax, and 81.0 ± 3.8 vs. 88.5 ± 1.1% CVCmax, respectively, all P < 0.05). Maximal forearm blood flow remained unchanged after heat acclimation (290.9 ± 12.7 vs. 269.9 ± 23.6 ml/min). The experimental group showed significant increases in sweating responses to 10 and 100 mM ACh (0.21 ± 0.03 vs. 0.31 ± 0.03 mg·cm−2·min−1 and 0.45 ± 0.05 vs. 0.67 ± 0.06 mg·cm−2·min−1, respectively, all P < 0.05), but not to 1 mM ACh (0.13 ± 0.02 vs. 0.18 ± 0.02 mg·cm−2·min−1, P = 0.147). No differences in any of the variables were found in the control group. Heat acclimation in highly trained subjects induced local adaptations within the skin microcirculation and sweat gland apparatus. Furthermore, maximal skin blood flow was not altered by heat acclimation, demonstrating that the observed changes were attributable to improvement in cutaneous vascular function and not to structural changes that limit maximal vasodilator capacity.

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