scholarly journals Antibacterial activity of Lippia alba, Myrcia lundianaand Ocimum basilicumessential oils against six food-spoiling pathogenic microorganisms

2021 ◽  
Vol 20 (3) ◽  
pp. 260-269
Author(s):  
Hyrla Grazielle Silva de Araujo Couto ◽  
◽  
Ana Andrea Teixeira Barbosa ◽  
Daniela Aparecida de Castro Nizio ◽  
Paulo Cesar de Lima Nogueira ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Pathogenic Bacteria ◽  
Ocimum Basilicum ◽  
Pathogenic Microorganisms ◽  
Diffusion Test ◽  
Lippia Alba ◽  
Screening Experiment ◽  
Three Samples ◽  
Agar Diffusion Test ◽  
Two Samples

The aim of this study was to undertake a screening experiment on essential oils (EO) of Myrcia lundiana, Ocimum basilicum and Lippia alba against six food-spoiling pathogenic bacteria. Seventy-two (72) samples were initially analyzed for antimicrobial activity based on the agar diffusion test. The minimum inhibitory (MIC) and bactericidal (MBC) concentrations were determined for the 12 samples which showed greatest antimicrobial potential in this stage. Two samples of L. alba, three samples of M. lundiana and seven samples of O. basilicum showed a MIC of 0.12-125 μL/mL for the six tested bacteria. Of these, the EO of O. basilicum cultivar Maria Bonita stood out with the lowest MIC and MBC. Thus, a mixture simulating this essential oil was prepared from commercial standards of the compounds (±)-linalool, geraniol and 1,8-cineole. Significantly higher MIC and MBC were detected in the simulation compared to the respective EO, suggesting a synergistic effectbetween compounds.

scholarly journals In Vitro Assessment for Antimicrobial Activity of Lactobacillus Helveticus and its Natural Glycopeptides Against Mastitis Causing Pathogens in Dairy Cat

2015 ◽  
Vol 9 (1) ◽  
pp. 61-66 ◽  
Author(s):  
Gundega Gulbe ◽  
Anda Valdovska ◽  
Vaira Saulite ◽  
Jevgenijs Jermolajevs
Keyword(s):  
Antimicrobial Activity ◽  
Lactic Acid ◽  
Pathogenic Bacteria ◽  
Antimicrobial Effect ◽  
Lactobacillus Helveticus ◽  
Haemolytic Activity ◽  
Pathogenic Microorganisms ◽  
Bacterial Strains ◽  
Coagulase Negative Staphylococci

Probiotic lactic acid bacteria have a great potential to control bovine mastitis as well as they are favourable choice to treat many infectious diseases of human. These bacteria are well known as having many properties which make them beneficial to control pathogenic microorganisms. These include, the ability to adhere to cell, the reduction of pathogenic bacteria adherents, the co–aggregation, the production of organic acids, hydrogen peroxide, bacteriocin and etc., to be safe and non–pathogenic, which antagonize pathogenic microorganisms. However, each strain must be well identified and characterized in vitro before using for disease treatment. The aim of the present study was to screen three kind of test suspensions: TS1, TS2 and TS3, which contains probiotic lactic acid bacterium Lactobacillus helveticus or its natural glycopeptides, and other natural immunomodulators, in order to investigate which content were the most effective in inhibiting several mastitis causing bacteria in dairy cattle: coagulase–positive Staphylococcus aureus, coagulase–negative staphylococci S. haemolyticus, S. saprophyticus, S. simulans, S. vitulinus, and Gram–negative bacteria Citrobacter freundii and Serratia liquefaciens. Test suspensions TS1, TS2 and TS3 were adjusted by pH 6.3, then tested in vitro by well diffusion assay to determine their antimicrobial effect against bacteria. Furthermore haemolytic activity of applied test suspensions were determined. In results TS1 (9-13 mm) and TS2 (10-15 mm) showed the inhibition effect on four of eight tested bacterial strains, whereas TS3 did not displayed any antimicrobial effect. TS2 have a greatest antimicrobial activity as they resulted in the largest inhibition zones.

scholarly journals 1-(2-aminophenyl)-1H-1,2,3-triazole-4-carboxylic acid: activity against Gram-positive and Gram-negative pathogens including Vibrio cholerae

2017 ◽  
Vol 4 (10) ◽  
pp. 170684 ◽  
Author(s):  
Krishnendu Maji ◽  
Debasish Haldar
Keyword(s):  
Antimicrobial Activity ◽  
Amino Acid ◽  
Carboxylic Acid ◽  
Vibrio Cholerae ◽  
Pathogenic Bacteria ◽  
Morphological Changes ◽  
Harmful Effect ◽  
Gram Positive ◽  
Gram Negative ◽  
Agar Diffusion Test

We report a new synthetic aromatic ε-amino acid containing a triazole moiety with antimicrobial potential against Gram-positive, Gram-negative and pathogenic bacteria including Vibrio cholerae . Structure–property relationship studies revealed that all the functional groups are essential to enhance the antimicrobial activity. The 1-(2-aminophenyl)-1H-1,2,3-triazole-4-carboxylic acid was synthesized by click chemistry. From X-ray crystallography, the amino acid adopts a kink-like structure where the phenyl and triazole rings are perpendicular to each other and the amine and acid groups maintain an angle of 60°. The agar diffusion test shows that the amino acid has significant antibacterial activity. The liquid culture test exhibits that the minimum inhibitory concentration (MIC) value for Bacillus subtilis and Vibrio cholerae is 59.5 µg ml −1 . FE-SEM experiments were performed to study the morphological changes of bacterial shape after treatment with compound 1 . The antimicrobial activity of the amino acid was further studied by DNA binding and degradation study, protein binding, dye-binding assay and morphological analysis. Moreover, the amino acid does not have any harmful effect on eukaryotes.

scholarly journals pH and Antimicrobial Activity of Portland Cement Associated with Different Radiopacifying Agents

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Juliane Maria Guerreiro-Tanomaru ◽  
Ana Lívia G. Cornélio ◽  
Carolina Andolfatto ◽  
Loise P. Salles ◽  
Mário Tanomaru-Filho
Keyword(s):  
Antimicrobial Activity ◽  
Portland Cement ◽  
Bismuth Oxide ◽  
Zirconium Oxide ◽  
Micrococcus Luteus ◽  
Diffusion Test ◽  
Calcium Tungstate ◽  
Ph Values ◽  
Agar Diffusion Test ◽  
Ph Increase

Objective. The aim of this study was to evaluate the antimicrobial activity and pH changes induced by Portland cement (PC) alone and in association with radiopacifiers. Methods. The materials tested were pure PC, PC + bismuth oxide, PC + zirconium oxide, PC + calcium tungstate, and zinc oxide and eugenol cement (ZOE). Antimicrobial activity was evaluated by agar diffusion test using the following strains: Micrococcus luteus, Streptococcus mutans, Enterococcus faecalis, Pseudomonas aeruginosa, and Candida albicans. After 24 hours of incubation at 37°C, inhibition of bacterial growth was observed and measured. For pH analysis, material samples () were placed in polyethylene tubes and immersed in 10 mL of distilled water. After 12, 24, 48, and 72 hours, the pH of the solutions was determined using a pH meter. Results. All microbial species were inhibited by the cements evaluated. All materials composed of PC with radiopacifying agents promoted pH increase similar to pure Portland cement. ZOE had the lowest pH values throughout all experimental periods. Conclusions. All Portland cement-based materials with the addition of different radiopacifiers (bismuth oxide, calcium tungstate, and zirconium oxide) presented antimicrobial activity and pH similar to pure Portland cement.

scholarly journals Isolation of pathogenic microorganisms from burn patient and in vitro determination of antibacterial activity of honey against antibiotic resistance isolates

2020 ◽  
Vol 6 (8) ◽  
pp. 310
Author(s):  
Ifra Tun Nur ◽  
Tahmina Jahan ◽  
Sharmin Akter
Keyword(s):  
Antimicrobial Activity ◽  
Pathogenic Bacteria ◽  
Diffusion Method ◽  
Pathogenic Microorganisms ◽  
Zone Of Inhibition ◽  
Burn Wound ◽  
College Hospital ◽  
Wide Range

<p class="abstract"><strong>Background:</strong> Honey is a natural therapeutic agent which manifest antimicrobial activity against a wide range of bacteria. Therefore, the current study was designed to isolate pathogenic bacteria from burn wound and also to determine the anti-bacterial traits of natural and processed honey against infectious agents.</p><p class="abstract"><strong>Methods:</strong> Wound samples were collected from burn unit of Dhaka Medical College Hospital and conventional cultural methods were applied to identify pathogenic microorganisms. A total of six samples including three each of natural and processed honey were tested for the determination of antimicrobial activity by agar well diffusion method.  </p><p class="abstract"><strong>Results:</strong> Among ten wound samples highest load of total viable bacteria was recorded up to 3.7×10<sup>6</sup> cfu/ml. The maximum load of <em>Pseudomonas </em>spp. and <em>Staphylococcus </em>spp. were found up to 1.6×10<sup>4</sup> cfu/ml and 8.7×10<sup>4</sup> cfu/ml respectively. Significant <em>in vitro</em> antimicrobial activity was found in all the samples. Natural honey showed a little bit more efficacy than processed honey. The samples exhibited antibacterial traits against <em>Staphylococcus aureus</em> with a wide zone of inhibition and moderate zone of inhibition against <em>Pseudomonas </em>spp. when they are subjected to 100% concentered honey. <em>E. coli</em> and <em>Klebsiella </em>spp. were remained to be unaffected at 75% and 50% concentrated honey, while <em>S. aureus</em> and <em>Pseudomonas </em>spp. were found to be sensitive at those concentrations.</p><p class="abstract"><strong>Conclusions:</strong> The <em>in vitro</em> efficacy of different types of honey tested against the bacteria dependent on the type of honey and the concentration at which it was administered. In our study 100% concentred honey was more efficient in inhibiting all the tested isolates.</p>

scholarly journals Antimicrobial properties of some essential oils against some pathogenic microorganisms

2008 ◽  
Vol 26 (No. 3) ◽  
pp. 174-181 ◽  
Author(s):  
N. Celikel ◽  
G. Kavas
Keyword(s):  
Essential Oils ◽  
Antimicrobial Properties ◽  
Natural Food ◽  
Pathogenic Microorganisms ◽  
Test Medium ◽  
Diffusion Test ◽  
Food Preservatives ◽  
Disk Diameter ◽  
Plant Essential Oils ◽  
Agar Diffusion Test

Investigations were carried out to assess the efficiency of five plant essential oils: thyme, myrtle, laurel, sage, and orange oils as natural food preservatives. The effect of the plant essential oils against <i>Escherichia coli</i>, <i>Listeria monocytogenes</i>, <i>Staphylococcus aureus</i> and <i>Candida albicans</i> at concentrations of 5–20 µl/disk (diameter 6 mm) and 0.5–3% (v/v) was studied in agar diffusion test medium and milk medium. The essential oils of these extracts exhibited markedly antibacterial and bacteriostatic activity, with thyme showing the highest inhibition and orange the lowest. However, with thyme extract, high inhibitory activity was observed for all tested concentrations, <i>L. monocytogenes</i> showed less sensitivity towards essential oil extracts.

scholarly journals Microbiological analysis for drug resistant pathogenic microorganisms with determination of the antibacterial properties found in Fragaria x ananassa (strawberry) samples

2017 ◽  
Vol 6 (1) ◽  
pp. 16-19
Author(s):  
Nazneen Piangka ◽  
Tasnia Ahmed ◽  
Mrityunjoy Acharjee
Keyword(s):  
Pathogenic Bacteria ◽  
Antibacterial Properties ◽  
Microbiological Analysis ◽  
Drug Resistant ◽  
E Coli ◽  
Three Samples ◽  
Wide Range ◽  
Two Samples ◽  
Health Related

Strawberry is a popular fruit item consumed by people all over the world for its exceptional flavor and aroma. This fruit is being used to prepare a wide range of food items. Strawberries when consumed by people without undergoing any processing, the condition may not be good enough. People may get sick after consuming raw strawberries. Our current study identified several pathogenic bacteria which are very known in causing health related problems. E. coli was found in one sample (1.0×102 cfu/g), Staphylococcus aureus was commonly present in all of the five samples (up to 1.2×106 cfu/g), Klebsiella spp. was found to be present in two samples (within 1.0×103 cfu/g) and Pseudomonas spp. was present in three samples (8.2×104 cfu/g), respectively. All isolated bacteria showed some degree of drug resistant traits against some commonly used antibiotics. The most important and promising part of the study was to successfully find the antimicrobial activity of strawberries against seven types of pathogenic bacteriaStamford Journal of Microbiology, Vol.6(1) 2016: 16-19

scholarly journals Evaluation of Antibacterial Properties of Triorganotin Carboxylates Containing Functionalised Ester Groups in Tests Against Some Pathogenic Bacteria

2001 ◽  
Vol 8 (2) ◽  
pp. 107-111 ◽  
Author(s):  
J. Koshy ◽  
A. Ansary ◽  
K. M. Lo ◽  
V. G. Kumar Das
Keyword(s):  
Pathogenic Bacteria ◽  
Antibacterial Properties ◽  
Plate Count ◽  
Gram Negative Bacteria ◽  
Diffusion Test ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Agar Diffusion Test ◽  
Ester Moiety ◽  
Triphenyltin Acetate

Bacterial screening employing the agar diffusion test on triphenyltin carboxylates containing various functional residues in the ester moiety revealed appreciable differences in their activities relative to triphenyltin acetate. Among these, [3-(Diethylphosphono)propionato] triphenyltin (1) and [N-cyclohexylcarbamoyl) glycinato] triphenyltin displayed activities comparable to tri-n-butyltin cinnamate (2) towards both Gram-positive and Gram-negative bacteria; the latter compound was the most active among the eleven triorganotin compounds tested, which included cyclopentyldiphenyltin hydroxide (3) and its methacrylate derivative. Applying the more quantitative plate count and optical density tests on compounds 1-3, it was shown that their inhibitory activity ranked in the order 2 > 3 >1. Significantly, 3 caused around 90% inhibition of both Eschechia coli (−) and Pseudomonas aeruginosa (−) when incubated for 24 h at 37±1℃ at the 10.0 μg/ mL concentration level. Compound 2 was less effective against P.aeruginosa than against E.coli. While the Gram-positive bacteria were all readily inhibited, Bacillus subtilis (+) appeared to the most susceptible among them towards the test compounds.

scholarly journals Antimicrobial, antibiofilm and biochemichal properties of Thymus vulgaris essential oil against clinical isolates of opportunistic infections

2019 ◽  
Vol 27 (3) ◽  
pp. 270-275 ◽  
Author(s):  
M. V. Kryvtsova ◽  
I. Salamon ◽  
J. Koscova ◽  
D. Bucko ◽  
M. Spivak
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Opportunistic Infections ◽  
Clinical Isolates ◽  
Thymus Vulgaris ◽  
Antibiofilm Activity ◽  
Diffusion Test ◽  
Antibiotic Resistant ◽  
Thyme Essential Oil ◽  
Agar Diffusion Test

Thyme belongs to a genus encompassing over 215 species of hardy perennial herbaceous plants and sub-shrubs, which are native to Europe, particularly around the Mediterranean. Thymus vulgaris L., or garden thyme, with narrow small leaves and clusters of tubular mauve flowers, is used mainly in cookery. Dried herb yields 1% and more essential oil, which is a pale yellowish-red liquid with a sweet, very aromatic odour. Thyme is widely used in the pharmaceutical industry and is a source of substances of antimicrobial effect upon antibiotic-resistant strains of microorganisms. The purpose of our work was to identify the biochemical and antimicrobial peculiarities of Th. vulgaris essential oil against clinical isolates of opportunistic microorganisms. The analysis of thyme essential oil was carried out using GC/MS analysis. The clinical isolates were isolated with the use of differentially diagnostic nutrient media. The antibiotic susceptibility was identified with the help of the disc-diffusion test. The sensitivity of microorganisms to plant extracts was determined by the agar diffusion test. The antibiofilm activity of the extracts was tested in standard 96-well microtitration plates. The GC/MS results confirm the earlier reports that the major volatile constituents obtained from the aerial parts of thyme species were thymol, γ-terpinene, p-cymene, 3-carene and carvacrol. After subjecting the selected essential oil to effective steam distillation, substantial contents of phenolic monoterpenoids were obtained – thymol (67.7%) and γ-terpinene (8.2%). The European Pharmacopoeia set quality standards for thyme essential oil, which dealt mainly with the % content (w/w) of the volatile phenols (expressed as thymol: 36.0–55.0%). Garden thyme essential oil has been found to show a high antimicrobial activity against antibiotic-resistant microorganism strains. The obtained results proved the wide spectrum of antibiotic activity of thyme essential oil. The highest antimicrobial activity was registered against the typical and clinic strains of S. aureus and microscopic Candida genus fungi. Garden thyme essential oil was ascertained to show high antibiofilm-forming activity against S. aureus. The antimicrobial and antibiofilm-forming activities of thyme essential oil against both bacterial pathogens of opportunistic infections and microscopic fungi have proven the good prospects for development of a broad-spectrum agent against opportunistic microbial associations based on this oil.

scholarly journals Silver Nanoparticles Impregnated Biocellulose Produced by Sweet Glutinous Rice Fermentation with the Genus Acetobacter

2020 ◽  
Vol 141 ◽  
pp. 03003 ◽  
Author(s):  
Duongruitai Nicomrat
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Antimicrobial Activities ◽  
Raw Material ◽  
Uniform Structure ◽  
Glutinous Rice ◽  
Agar Diffusion Test ◽  
Two Samples ◽  
Static Cultivation

Bacterial cellulose (BC) is a natural renewable and biodegradable polymer and mostly synthesized from the bacteria genus, Acetobacter. More recent studies on effective biopolymer in various applications have been conducted due to its uniform structure and morphology superior to those of plant cellulose. In this study, the researchers have observed the effectiveness on antimicrobial activity of BC produced by sweet glutinous rice as a raw material instead of cellulosic materials. In the process, sweet glutinous rice fermented by Loog-pang was fermented for alcohol before further making rice vinegar product by inoculating with Gluconacetobacter xylinum TISTR 086, or mixed with indigenous vinegar producers, isolated Acetobacter spp. from our laboratory. Both these two samples gave comparable BC yields and the antimicrobial activity after impregnating with silver nanoparticles and also comparable to the control coconut vinegar medium (2.20 vs 2.00 g/L within 3 days). The optimum conditions for high BC production were pH 4 to 7, temperature approximately 25-30°C, slow agitation (50 to 90 rpm) as well as static cultivation. The remarkable antimicrobial activities of silver nanoparticles impregnated BC products (Ag-BC) showed high tentative in vitro inhibition on common pathogens in contaminated wounds; Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa by agar diffusion test. From this study, Ag-BC produced by fermented sweet glutinous rice (FSR) will therefore be one attractive affordable choice for being biomaterials applied in medical interests, especially skin disinfectant.

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