Hematological Indicators of Moose in Domestication Process

Presented hematological indices for a stress-free selection of material from moose in the process of domestication depending on age and sex characteristics. Fluctuations in the blood parameters of moose have been established within various limits, taking into account the influence of various factors - the climatic, environmental, technological, physiological state of the animal, and age and sex characteristics. Considerable attention is paid to the selection of blood from moose, one of the most labor-intensive processes when working with them. Some tendency to fluctuations in hematological parameters in young moose has been recorded. This, possibly, is associated with a decrease in the action of colostral immunity, the formation of a specific defense of the body. Differences in blood indices in moose depending on gender were unreliable. However, in males, some of them - in terms of the content of erythrocytes, basophils, and monocytes - was higher than in females, and vice versa, in females - the number of leukocytes, lymphocytes, and eosinophils. It was shown that in young animals of 21-50 days of age, a neutrophilic profile prevails with increased content of segmented forms, and in adults - a lymphocytic profile. Compared with moose, which are in the process of domestication in animals living in natural conditions, expressed leukocytosis was recorded. As a result of the studies, the estimated norms of morphological parameters of the blood of moose in the process of domestication are established, depending on age and gender.

2017 ◽  
Vol 19 (74) ◽  
pp. 24-28
Author(s):  
N. I. Hradovych

Many authors noticed the actuality of fish hematological parameters usage as indices of anthropogenic influence. Blood system reflects the reaction of the body on the action of different ecological factors. The problem of heavy metals influence on hematological parameters of silver cap (Hypopthalmichtys molitrix) needs to be investigated. The aim of the work was studying of ecotoxical Plumbum and Cadmium influence on hematological indices of silver carp (Hypopthalmichtys molitrix) during the period exposition. The results of the investigations concerning the influence of Plumbum and Cadmium on some hematological indices of silver carp (Hypopthalmichtys molitrix) for 12 days exposition up to the action of these heavy metals are given in this article. Experimental part was realized at the Lviv research station of Institute of fisheries NAAS (Lviv oblast, Horodok region, Velykyi Lyubin). Experimental groups contained 10 two-years individuals each. Fish were adopted to laboratory conditions for 5 days under the temperature 19 ± 2ºС, рН = 7.3 ± 0.2, oxygen content 7–8 mg/l. Blood parameters were measured on the sixth and twelfth days at the control group and groups which were under the influence on the level of 2 and 5 MPC of the appropriate metal. It was proved that the influence of Plumbum and Cadmium causes the lowering of erythrocytes number, hemoglobin and corpuscular volume: the effect becomes stronger when he concentration increases and in time. The effect from intoxication by Cadmium comes faster than by Plumbum.


Author(s):  
N.S. Khokhlova ◽  
◽  
V.V. Semenyutin ◽  
◽  

The influence of the physiological state of rabbits (pregnancy and lactation) on the hematological parameters of blood during changes in the area of the plantar surface of the feet of the hind limbs in conditions of cage keeping was studied. In the body of pregnant females, with the appearance of signs of hyperkeratosis and alopecia in the studied area of the feet, an increase in hemoglobin level occurs, an increase in MCH (mean corpuscular hemoglobin), MCV (Mean Corpuscular Volume), MCHC (corpuscular hemoglobin concentration) occurs, followed by a decrease in the development of hemorrhagic changes. The level of red blood cells has slight fluctuations. In lactating females, with the occurrence of alopecia and hyperkeratosis, an increase in the level of erythrocytes and hematocrit occurs with a simultaneous decrease in hemoglobin, and against the background of the development of hemorrhages, these indicators decrease, a decline in the values of MCH, MCHS and an increase in MCV are recorded. Regardless of the physiological state of the animal and changes in the area of the feet, the erythrocyte sedimentation rate (ESR) value and the level of leukocytes fluctuate within the limits of physiological values, which indicates the absence of an inflammatory process in the body. he revealed hematological changes indicate the development of adaptive processes in the event of hyperkeratosis and alopecia in the area of the plantar surface of the feet at various physiological conditions of rabbits with subsequent decompensating against the background of hemorrhagic changes.


Author(s):  
D.D. ADZHIYEV ◽  
◽  
S.A. RUMYANTSEV ◽  
G.I. PRONINA ◽  
N.A. SAPOZHNIKOVA ◽  
...  

Despite the multifactorial oxidant-antioxidant interactions in the body, it is necessary to determine the antioxidant status as the total balance of free radical generation processes and the activity of the enzymatic and nonenzymatic defense lines of the body. In this regard, an important diagnostic problem is the selection of adequate indicators that reflect both components. Obviously, these indicators should be stable and multiple. However, there is another problem associated with the selection of integrated indicators that simplify the information assessment of the antioxidant status of the body. The aim of this research work was to study the intensity of lipid peroxidation and changes in the antioxidant system in intact rabbits of different age groups and sex in postnatal ontogenesis. The studies were carried out on 30 (15 males and 15 females) rabbits in bioclinics. Blood was taken from animals aged 60, 120 and 180 days. The content of diene conjugates and Malon dialdehyde, as well as low-molecular antioxidants – tocopherol and retinol – were determined in blood plasma. The activity of antioxidant enzymes – glutathione reductase, glutathione peroxidase, superoxide dismutase and catalase was evaluated in hemolysates. Thus, the activity of SOD, CAT, GP, GR and the concentration of DC and MDA in some cases significantly decreased in all studied groups of animals by the end of the experiment. The content of cholesterol, triglycerides and glucose shows the sexual differences between male and female rabbits in the lipid and energy spectrum of blood plasma. At the age of 180 days, the increase in cholesterol, triglycerides and glucose in males amounted to 13.1%, 37.4% (at P ≤ 0.001) and 14.1%. Accordingly, for females: 17.2%, 37.8% (at P ≤ 0.001) and 15.8%. At the age of 120 and 180 days there was an unreliable decrease in all indicators in animals as compared with the values obtained in the 60-day age. The content of tocopherol in males in 120 and 180 days increased by 17.9 (at P ≤ 0.001) and 21.2% (at P ≤ 0.001), retinol – by 45.5% (at P ≤ 0.01) and 63.6% (at P ≤ 0.01) as contrasted to to the initial (60 days) values. Similarly, females showed a significant increase in the concentration of tocopherol by 20.7% (at P ≤ 0.001) and 23.3% (at P ≤ 0.001), retinol – by 30.8% (at P ≤ 0.05) in 180 days. Thus, the influence of age and sex on the indicators of peroxide and lipid metabolism, the content of fat-soluble vitamins A and E in blood plasma, the activity of the main enzymes of antioxidant protection of animals has been shown. According to the content of cholesterol and triglycerides, sexual differences between male and female rabbits in the lipid spectrum of blood plasma have been established. This, in turn, helped determine the specific weight of individual factors of the AOS-POL system in the ontogenesis of rabbits.


2020 ◽  
Vol 22 (98) ◽  
pp. 42-45
Author(s):  
L. V. Koreyba ◽  
Y. V. Duda

The requirement for the study of blood parameters is determined by its physiological significance and changes that occur in different physiological and pathological conditions. The hematological profile is an important criterion that shows the physiological condition of animals. The physiological changes of blood in cows have been established concerning the conditions of keeping and feeding, hygienic parameters, in the dynamics of the dry season, in different seasons of the year, etcetera. We have not found any information on studies of the dynamics of blood parameters in pregnant animals, depending on their age, in the study of literary sources in recent years. The purpose of our work was to study the age-related dynamics of hematological blood parameters in cows during the dry period. Our studies aimed to determine the hematological indices of Holstein breed cows in the physiological course of the prenatal period during the dry period, depending on the number of deep calving. Control and experimental groups were formed on the principle of group-analogues (by breed, age, sex, physiological state, weight, milk productivity). Animals kept in typical rooms with satisfactory microclimate parameters (in winter) and at camps (in summer). Laboratory studies were carried out in the laboratory of the Dnipro State Agrarian and Economic University. Hematologic studies were performed according to the following indicators: erythrocytes, hemoglobin, color index, erythrocyte sedimentation rate, leukocytes, monocytes, lymphocytes, neutrophils with rod-like shape and segmented nucleus, basophils and eosinophils according to conventional methods. The results of our studies indicate that the lowest number of erythrocytes was detected in the cows of the sixth calving and the highest hemoglobin content, which led to the rise of the color index – in cows of the fifth pregnancy present the reverse tendency; increase to the maximum values of the number of leukocytes in cows of the third body (1.32 times), which is a reflection of the greater reactivity of the body of cows of this age group; a probable decrease (by 10.59 %, P < 0.05) of lymphocyte counts was found in sixth-calf cows, indicating a weakened immune response; also increased eosinophils almost 1.75 times (P < 0.05) compared to fifth pregnancy; the trend of a gradual decrease in the number of segmented neutrophils in calving cows is followed by a second to fifth pregnancy and a significant reduction in the number of stab neutrophils in cows of the fifth pregnancy of up to 1.33 %.


2019 ◽  
Vol 51 (3) ◽  
pp. 26-31
Author(s):  
E.A. Azhmuldinov ◽  
◽  
Yu.N. Chernyshenko ◽  
M.G. Titov ◽  
◽  
...  

A study was conducted of the effect of heat stress on the clinical and physiological state of male rabbits at the age of 5 months with an average live weight of 1551 g. The maximum temperature the animals were exposed to during the experiment was +42 °C. The study results showed a decrease in feed intake and an increase in water consumption, which contributed to a decrease in live weight. Hematological parameters, including hemoglobin, erythrocytes, leukocytes, total plasma protein, globulins and cholesterol increased during the action of this stress factor. It was found that the effect of high ambient temperatures on rabbits negatively affected their physiological functions; the negative effect was reflected by the morphological and biochemical blood parameters.


2019 ◽  
Vol 12 (1) ◽  
pp. 97-106
Author(s):  
AL-Khafaji Nazar

Ricinus communis L. of Euphorbiaceae family is a widespread plant in tropical regions. It is used in traditional medicines as an anti- fertility agent in India and different parts of the world. The ether soluble portion of the methanol extract of R. communis var minor possesses anti-implantation, anti - conceptive and estrogenic activity in rats and mice when administered subcutaneously.The study was conducted on 10 local breed male rabbits, 1-2 years old, of 1-2 kg body weight. The animals were divided into two groups, control non – treated group and treated group in which animals were treated with single daily dose of 50 mg /kg b. wt. P.O. of decorticated and defatted castor seeds (DDCS) for 14 days. 28th day post treatment, animals were anesthetized by diethyl ether, sacrificed, abdominal cavity was open. The sexual organ (testes, epididymis, prostate and seminal vesical) weighed. In addition to take a biopsy from each one for histopathological changes. The study also included clinical and hematological parameters, in addition to sperm counts and the changes in sperm morphology.Body weight, body temperature increased significantly in treated males. While in non- treated group there were no significant changes. Respiratory rates and heart rate were none significantly changed in treated and non- treated males.Bleeding time none significantly increased in treated males, but increased significantly in none treated males. Clotting times decreased none significantly in treated and non- treated males. The blood parameters including, total erythrocytes count, hemoglobin concentration, PCV% , MCV, MCH, MCHC, total leucocyte and differential leucocyte counts were either increased or decreased none significantly in both groups. The results revealed that the effects of exposure to extract of ricin for 14 days on reproductive efficiency of rabbits, exhibited Significant decrease in weights of testes, epididymis, tails, heads of epididymis, seminal vesicles and prostate in treated males in comparison with those of non- treated males. While the body of epididymis did not show a significant changes.Significant decrease in live sperm numbers, number of sperms in epididymal head, in addition to deformities in high numbers of sperm, including enlarged or small sperms. breaks head, and its detachment, presence of two heads in one sperm, bifurcation of tail and its breaking, sperm coiling in samples from treated males in comparison with those from non-treated males.Histological changes were hyperplasia of lining epithelial cells and vacuolar degenerative changes, loss of spermatogenesis, and spermatocytes necrosis in those from treated males.


2020 ◽  
Vol 7 (3) ◽  
pp. 439-444
Author(s):  
Md Alamgir Kobir ◽  
Latifa Akther ◽  
Imam Hasan ◽  
Md Ahosanul Haque Shahid ◽  
Ziaul Haque ◽  
...  

Blood parameters are an important index to assess the body homeostasis in mammals. Here, the effect of pesticide (imidacloprid) contaminated feed exposure on the haematological parameters of adult rabbits (n=15) was studied. Pesticides (Imidacloprid, Bildor® 0.5ml (100mg)/L water spray on vegetables and green grass) exposed feed fed to rabbits with wash (washed feed rabbit) or without a wash (not washed feed rabbit) in the once-daily morning for every alternative day up to 15 days. Control rabbits have received a standard diet (fresh vegetables and green grass). The blood cell analysis showed that the total erythrocyte count, packed cell volume, and haemoglobin values were not changed significantly in pesticide exposed rabbit. For differential leukocyte count, the percentage of neutrophil and eosinophil was significantly decreased in the pesticide-exposed rabbit as compared to the control rabbit. The lymphocyte percentage was increased significantly in pesticide-exposed rabbits compared to control rabbits. Interestingly, the number of lymphocytes was significantly increased in not washed feed rabbits compared to wash feed rabbits. The results of the present study suggest that residue of agriculturally used pesticides may affect the body homeostasis of animals. Res. Agric., Livest. Fish.7(3): 439-444,  December 2020


Author(s):  
S. S. Mirgalimova ◽  
A. O. Trufanova ◽  
R. R. Fatkullin

The physiological state of animals is to a certain extent characterized by hematological indicators, since blood occupies a special place in the body. By transferring nutrients and biologically active substances, the blood performs the general regulation of vital functions of the body. The use of Vitartil as a feed additive in the diets of animals leads to an increase in glycolytic processes and a decrease in the body’s need for oxygen delivery, which contributes to the growth of the animal body. The purpose of the research was to study the changes in blood parameters taking into account the age of Hereford steers against the background of the use of Vitartil feed additive to the main diet and its effect on the productive traits of animals. In order to carry out the experiment two groups of steers-analogs of Hereford breed have been formed at the age of 6 months per 10 heads in each. The difference between the groups was that the steers of the experimental group have been received the feed additive Vitartil in addition to the main diet. The greater yield of the meat part was in the steers of the experimental group in comparison with the herdmates of the control group. This indicator in the experimental group was 78,9 %, while in the control group it was lower by 1,6 %. The animals of the experimental group were superior to their herdmates in terms of meat weight by 1,4 kg or by 8,8 %. The relative number of bones was lower in the experimental group and amounted to 18,4 %, and in the control group 19,2 %. The meat content index was high in the steer have been received the biologically active additive Vitartil and was 4,3, and in the control group 4,0. It has been found that the use of Vitartil feed additive to the main diet of Hereford steers increases the blood content of hemoglobin and glucose, which has a certain effect on the morphological and chemical composition of meat, as well as increases its energy value.


2019 ◽  
Vol 75 (05) ◽  
pp. 6256-2019
Author(s):  
KRZYSZTOF PATKOWSKI ◽  
KATARZYNA OGNIK ◽  
MARIUSZ KULIK ◽  
MONIKA GREGUŁA – KANIA ◽  
TOMASZ M. GRUSZECKI

The aim of the study was to determine the effect of sheep’s genotype and grazing site on their hematological and redox status indicators. The experiment was carried out on sheep of the Świniarka and Uhruska breeds grazed in naturally valuable areas (xerothermic grasslands) and kept in a sheepfold. Several blood indices were determined by ABACUS Junior Vet, including the numbers of leukocytes, thrombocytes and erythrocytes, hemoglobin and procalcitonin concentrations, hematocrit, the mean red cell volume and the average hemoglobin concentration in red blood cells, the percentages of lymphocytes, monocytes and granulocytes, the degree of erythrocyte anisocytosis and the degree of variation in platelet volume. The concentrations of lipid peroxides and malonic dialdehyde, the activity of superoxide dismutase and catalase and total antioxidant potential were determined by spectrophotometric methods. The study showed that Świniarka sheep had a greater antioxidant potential and higher white blood cell counts than Uhrusian sheep. It was found that sheep’s grazing place had a significant influence on the antioxidant status and hematological indicators of their blood. An improvement in antioxidant potential and hematological blood parameters was achieved in sheep grazed in naturally valuable areas, that is, in the Stawska Góra nature reserve and on xerothermic grasslands in Gródek and Kąty, compared to sheep fed in a sheepfold


2021 ◽  
Vol 23 (102) ◽  
pp. 78-86
Author(s):  
T. P. Todosiuk ◽  
M. V. Rublenko ◽  
V. M. Vlasenko

Bone tissue has powerful regenerative properties, thanks to which, with stable fixation, quite large amounts of skeletal bone damage can be successfully repaired. However, in the case of fragmentary fractures, the use of osteosynthesis methods alone does not always ensure the optimal course of reparative regeneration, as its regenerative potential is lost. Therefore, there is a need to replace post-traumatic bone defects and stimulate reparative osteogenesis. For this purpose, doped or doped with various elements (Ge, Si, Zn, Ag, Cu) composite materials. The aim of the study was to investigate the dynamics of hematological parameters in rabbits for osteosubstitution by hydroxyapatite ceramics doped with germanium and in combination with a blood clotting activator. Model defects were formed in the radial diaphysis and femur metaphysis in rabbits with a 3 mm and 4.2 mm diameter drill bit, respectively. Anesthesia included acepromazine, thiopenate, and lidocaine infiltration anesthesia. Animals of the first experimental group (n = 12) were replaced by defects with granules of hydroxyapatite ceramics doped with germanium (HTGe), the second (n = 12) – hydroxyapatite ceramics doped with germanium with blood coagulation activator (HTGe + a), the third – (n = 12). hydroxyapatite α + β with active (α + β + a), and control – granules of undoped ceramics (HT). On day 7, animals of all groups had minor post-traumatic erythrocytopenia and oligochromemia. There was also a gradual increase in the content of leukocytes in the blood with a peak on the 14th day, which in the group HTGe + a lasted until the 30th day. The increase in the number of leukocytes and their peak values occurred within the physiological norm and only approached its upper limit. In most groups there was a pronounced thrombocytosis during the first 14 days with normalization to the 30th day, but in the 2nd experimental group the number of platelets returned to normal only on the 60th day. Changes in most integral hematological indices, which reflect the relationship between blood cell populations, are characteristic of the inflammatory-resorptive phase of reparative osteogenesis. The dynamics of hematological parameters indicates the absence of a pronounced reaction of the body to the implantation of hydroxyapatite composite doped with germanium. The combination of hydroxyapatite ceramics doped with germanium with coagulation activator eliminates post-traumatic oligochromemia and erythrocytopenia and significantly increases the level of hematological integral indices, which indicates a more intensive course of inflammatory-resorptive clinical resorptive aparation phase.


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