scholarly journals Ecotoxical influence of plumbum and cadmіum on gematological parameters of sylver carp (hypopthalmichtys molitrix)

2017 ◽  
Vol 19 (74) ◽  
pp. 24-28
Author(s):  
N. I. Hradovych
Keyword(s):  
Heavy Metals ◽  
Hematological Parameters ◽  
Silver Carp ◽  
Ecological Factors ◽  
Blood Parameters ◽  
The Body ◽  
Control Group ◽  
Research Station ◽  
Hematological Indices ◽  
Experimental Part

Many authors noticed the actuality of fish hematological parameters usage as indices of anthropogenic influence. Blood system reflects the reaction of the body on the action of different ecological factors. The problem of heavy metals influence on hematological parameters of silver cap (Hypopthalmichtys molitrix) needs to be investigated. The aim of the work was studying of ecotoxical Plumbum and Cadmium influence on hematological indices of silver carp (Hypopthalmichtys molitrix) during the period exposition. The results of the investigations concerning the influence of Plumbum and Cadmium on some hematological indices of silver carp (Hypopthalmichtys molitrix) for 12 days exposition up to the action of these heavy metals are given in this article. Experimental part was realized at the Lviv research station of Institute of fisheries NAAS (Lviv oblast, Horodok region, Velykyi Lyubin). Experimental groups contained 10 two-years individuals each. Fish were adopted to laboratory conditions for 5 days under the temperature 19 ± 2ºС, рН = 7.3 ± 0.2, oxygen content 7–8 mg/l. Blood parameters were measured on the sixth and twelfth days at the control group and groups which were under the influence on the level of 2 and 5 MPC of the appropriate metal. It was proved that the influence of Plumbum and Cadmium causes the lowering of erythrocytes number, hemoglobin and corpuscular volume: the effect becomes stronger when he concentration increases and in time. The effect from intoxication by Cadmium comes faster than by Plumbum.

Hematological Indicators of Moose in Domestication Process

2020 ◽  
Keyword(s):  
Hematological Parameters ◽  
Physiological State ◽  
Blood Parameters ◽  
The Body ◽  
Sex Characteristics ◽  
Hematological Indices ◽  
Blood Indices ◽  
Hematological Indicators ◽  
Age And Sex ◽  
Selection Of

Presented hematological indices for a stress-free selection of material from moose in the process of domestication depending on age and sex characteristics. Fluctuations in the blood parameters of moose have been established within various limits, taking into account the influence of various factors - the climatic, environmental, technological, physiological state of the animal, and age and sex characteristics. Considerable attention is paid to the selection of blood from moose, one of the most labor-intensive processes when working with them. Some tendency to fluctuations in hematological parameters in young moose has been recorded. This, possibly, is associated with a decrease in the action of colostral immunity, the formation of a specific defense of the body. Differences in blood indices in moose depending on gender were unreliable. However, in males, some of them - in terms of the content of erythrocytes, basophils, and monocytes - was higher than in females, and vice versa, in females - the number of leukocytes, lymphocytes, and eosinophils. It was shown that in young animals of 21-50 days of age, a neutrophilic profile prevails with increased content of segmented forms, and in adults - a lymphocytic profile. Compared with moose, which are in the process of domestication in animals living in natural conditions, expressed leukocytosis was recorded. As a result of the studies, the estimated norms of morphological parameters of the blood of moose in the process of domestication are established, depending on age and gender.

scholarly journals Antioxidant Effect of Saccharomyces cerevisiae Enriched with Selenium Against Pseudomonas aeruginosa Infection

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Khadijeh Mansouri-Habibabadi ◽  
Mahnoosh Fatemi ◽  
Fereshteh Ghandehari
Keyword(s):  
Oxidative Stress ◽  
Saccharomyces Cerevisiae ◽  
Pseudomonas Aeruginosa ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Blood Parameters ◽  
The Body ◽  
Control Group ◽  
Antioxidant Agents ◽  
Total Antioxidant

Background: The reaction of immune cells to the introduction of pathogens into the body is an increase in reactive oxygen species, which leads to the induction of oxidative stress. Reducing the level of oxidative stress through the use of antioxidants strengthens the immune system. Objectives: This study aimed to investigate the antioxidant effects of Saccharomyces cerevisiae and selenium-enriched S. cerevisiae against the oxidative stress induced by Pseudomonas aeruginosa in rats. Methods: A total of 36 rats were randomly divided into six groups, including control (A), S. cerevisiae (B), S. cerevisiae enriched with selenium (C), P. aeruginosa infection treated with S. cerevisiae (D), P. aeruginosa infection treated with S. cerevisiae enriched with selenium (E), and P. aeruginosa infection (F). At the end of the treatment period, the blood samples were collected to evaluate hematological parameters, total antioxidant capacity, glutathione concentration, and glutathione peroxidase activity. Results: The concentration and activity of antioxidant agents in group F were significantly decreased compared to those of the control group; however, in groups D and E, the levels of the aforementioned factors showed a significant increase compared to those of the infected group. The number of white blood cells in group F showed a significant increase, compared to that of the control group. In other groups, the observed differences in antioxidant factors and blood parameters were not significant, compared to those reported for the control group. Conclusions: The results showed that the induction of oxidative stress by P. aeruginosa was partially reduced by treatment with yeast and selenium-enriched yeast. Further studies are essential to confirm the aforementioned results.

scholarly journals DYNAMICS OF HEMATOLOGICAL INDICES IN RATS AFTER ADMINISTRATION OF DIAMOND-CARBON COATED STEEL IMPLANTS INTO THE FEMUR

2019 ◽  
Vol 19 (3) ◽  
pp. 138-141
Author(s):  
V.B. Makarov ◽  
D.V. Morozenko ◽  
K. B. Gliebova
Keyword(s):  
Hematological Parameters ◽  
White Blood Cells ◽  
Test Group ◽  
The Body ◽  
Male Rats ◽  
Control Group ◽  
Coated Steel ◽  
Hematological Indices ◽  
Metal Implants ◽  
Body Response

Introduction. The pathological body response to metal implants is considered to be well known. There are a lot of studies devoted to this issue. This work is devoted to investigating the dynamic changes of haematological indicators as markers reflecting body response to the insertion of steel implants with diamond-carbon coating in rats. The purpose of the study is to assess the effects caused by steel implant with a diamond-coated coating on rat’s body by assessing hematological parameters. Materials and methods. The study involved 61 male rats: five of which were intact animals, and the rest was divided into two groups (28 animals in each). The first group was the control group, in which rats were inserted non-coated steel implants into the femoral metaphysic; the second group was test group, in which rats were inserted steel diamond-coated implants). The age of animals at the beginning of the experiment was 5–6 months, the body weight was 300–400 g. Blood for research was taken from the animals after decapitation in 7, 14, 30 and 90 days following the implantation. The blood samples were measured for the red blood cell, white blood cells, haemoglobin, leukogram. The findings obtained were processed by statistical analysis methods. Results and conclusions. The first group of the rats with inserted steel implants without coating showed the number of erythrocytes and haemoglobin did not differ from those in the intact rats, the leukocyte count on the 7th and 14th days was reduced by 31.8 % to 39.4 %, respectively; on the 7th day the percentage of lymphocytes and monocytes increased by 13.0 % and 5.0 % due to the toxic immunosuppressive effect of steel implants on the body of the experimental animals. The 2nd group of rats, which were inserted diamond-coated steel implants in their femurs, the rates of erythrocytopoiesis, the leukocyte number and leukogram characteristics remained unchanged during all the observation periods, except for an increase in the percentage of eosinophils on the 7th day and lymphocytes on the 90th day of follow- by 3 % that indicates a less pronounced body response in comparison with the animals of I group.

Erythrocyte and reticulocyte parameters during biological monitoring of lead exposure to the body

2020 ◽  
Vol 60 (11) ◽  
pp. 873-876
Author(s):  
Anastasiya G. Khotuleva ◽  
Mariya S. Kozyreva
Keyword(s):  
Biological Monitoring ◽  
Hematological Parameters ◽  
Lead Level ◽  
Statistical Processing ◽  
Absorption Spectrometry ◽  
The Body ◽  
Control Group ◽  
Processing Plant ◽  
Hematological Disorders ◽  
Globin Synthesis

Introduction. The most susceptible to lead is the hematopoietic system of hematopoietic organs due to lead inhibition of heme and globin synthesis and cytotoxic effect on the membrane of Mature red blood cells. The aim of study was to evaluate the informative value of the study of erythrocyte and reticulocyte parameters determined on modern hematological analyzers in patients working in contact with lead during medical and biological monitoring. Materials and methods. 45 employees of the lead battery processing plant and 30 persons of control group were examined. The level of lead in the blood was determined by atomic absorption spectrometry, δ-ALA in the urine-by the reaction of pyrol formation with acetylacetone in terms of gram of creatinine, the study of hematological parameters was performed on a Sysmex HT-2000i analyzer. Statistical processing of the results was performed using the program STATISTICA 10.0. Results. Significant changes in erythrocytic (RDW) and reticulocytic (RET, IRF, LFR, MFR, HFR, RET-He) parameters, erythropoietin in workers in contact with lead compared to the control group, changes in MCV, MCH, RDW, RET indicators in the group working in dynamics after 2 years were revealed. Associations of hematological parameters with biomarkers of exposure and effect (lead level in blood and ALA in urine) were revealed. Conclusions. Assessment of erythrocyte (MCV, MCH, RDW) and reticulocyte parameters (RET% and their distribution by maturity) in dynamics during periodic medical examinations of workers in contact with lead allows us to detect the development of hematological disorders at early stages.

scholarly journals RESVERATROL INCLUSION COMPLEX WITH β-CYCLODEXTRIN (RCD): CHARACTERIZATION AND EVALUATION OF TOXICITY IN WISTAR RATS

2016 ◽  
Vol 9 (1) ◽  
pp. 27 ◽  
Author(s):  
V. S. K. Nishihira ◽  
N. J. Mezzomo ◽  
M. D. Baldissera ◽  
R. A. Vaucher ◽  
C. G. Pinto ◽  
...  
Keyword(s):  
Inclusion Complex ◽  
Oral Administration ◽  
Biochemical Parameters ◽  
Wistar Rats ◽  
Metabolic Diseases ◽  
Hematological Parameters ◽  
Density Lipoprotein ◽  
Blood Parameters ◽  
Control Group ◽  
Scanning Calorimetry

<p class="RSCB01ARTAbstract"><strong>Objective</strong>:<strong> </strong>The aim of this study was to characterise the resveratrol inclusion complex with β-cyclodextrin (RCD) and evaluate their toxicity in wistar rats.</p><p class="RSCB01ARTAbstract"><strong>Methods: </strong>The RCD were prepared in ultra-turrax. For characterization of the RCD were used: Fourier transform infra-red Spectroscopy, Nuclear Magnetic Resonance (NMR), Differential Scanning Calorimetry (DSC) and X-ray powder diffraction. The RCD and others 4 treatments were performed by the chronic oral administration in 35 rats during 60 ds. After the treatments they were euthanized and the serum blood were collected to analyzed some hemogram and biochemical parameters including aspartyl aminotransferase (AST); alanine aminotransferase (AST); phosphatase alkaline (ALP); total bilirubin (TB); direct bilirubin (DB); total protein (TP); total cholesterol (TC), triacylglycerol (TAG), very low-density lipoprotein (VLDL), high-density lipoprotein (HDL), calcium, iron and phosphate using fully automated biochemistry analyzer.</p><p class="RSCB01ARTAbstract"><strong>Results: </strong>The characterization results indicated a successful formation of the RCD. All hematological parameters analysed were within the normal values in all the groups. Furthermore, the hemogram and biochemical parameters were significantly (P&gt;0.05) similar to the control group.</p><p class="RSCB01ARTAbstract"><strong>Conclusion: </strong>The daily oral administration during 60 d of RCD are not harmful on blood parameters of Wistar rats. Thus, RCD can be used safely for treatment of some metabolic diseases.</p>

scholarly journals Hematological parameters of carp juveniles in terms of introducing Quercus cortex extract into diet

2019 ◽  
Vol 2019 (4) ◽  
pp. 124-131
Author(s):  
Elena Petrovna Miroshnikova ◽  
Julia Vladimirovna Kilyakova ◽  
Sergey Vladimirovich Ponomarev ◽  
Azamat Ersainovich Arinzhanov ◽  
Maria Sergeevna Miroshnikova
Keyword(s):  
Hematological Parameters ◽  
Blood Parameters ◽  
Average Concentration ◽  
Control Group ◽  
Physiological Status ◽  
Metabolic Processes ◽  
Maximum Increase ◽  
Dehydrogenase Enzyme ◽  
Feed Analysis ◽  
Experimental Group

The article presents the results of studies of morphological and biochemical blood parameters of carp juveniles when adding the extract Quercus cortex (1, 2, 3 mg/kg of feed), probiotic preparation of Soya-bifidum (0.7 ml/kg of feed), antibiotics Ciprofloxacin hydrochloride in the composition of the drug Antibak 250 (100 mg/kg of feed). Analysis of hematological parameters of carp juveniles showed that adding Quercus cortex extract to the diet at a dosage of 2 mg/kg of feed contributed to increasing SGE by 66%, the average concentration of hemoglobin in the red blood cell by 20% compared to the control group. An increase in the level of leukocytes compared to the control was recorded only in the IV experimental group and made 13% (P≤0.05). An increase in glucose in the groups receiving Quercus cortex extract has been stated: in the I experimental group- by 4.2 times (P≤0.001), in II – by 3.4 (P≤0.001) and in III – by 3.3 (P≤0.001) compared to the control. Increasing glucose concentration indicates active metabolic processes and better stress resistance in the fish body. Iron content in carp blood was higher only in the first experimental group (by 3 times) compared to the control. Increasing activity of alanine aminotransferase was observed in all experimental groups, the highest rate of this enzyme being recorded in the IV experimental group (feed with probiotic additive Soya-bifidum) with a significant excess of control by 48%. Lactate dehydrogenase enzyme activity exceeded the control in all experimental groups, maximum increase (by 67%) was observed in the IV experimental group. Alkaline phosphatase was below the control in all experimental groups, minimum values being registered in the IV experimental group- by 37% less than the control. Indicators of p-amylase in the IV group exceeded the control by 2.6 times, in the V group – by 1.5 times. In the II group p-amylase was lower than the control by 44%, and in the III – by 1.9 times. According to the obtained hematological data, it has been established that including Quercus cortex extract into the diet of carp juveniles is promising due to the positive effect on the fish physiological status, immune status, metabolic processes, hematopoiesis and digestibility of feed.

scholarly journals Metallothionein and Glutathione Content as Biomarkers of Metal Pollution in Mussels and Local Fishermen in Abu Qir Bay, Egypt

2016 ◽  
Vol 6 (12) ◽  
pp. 50-60 ◽  
Author(s):  
Aziza A. Saad ◽  
Amany El-Sikaily ◽  
Hany Kassem
Keyword(s):  
Oxidative Stress ◽  
Heavy Metals ◽  
Metal Pollution ◽  
The Body ◽  
Metal Exposure ◽  
Control Group ◽  
Blood Levels ◽  
Industrial Workers ◽  
High Concentrations ◽  
Abu Qir Bay

Background. When heavy metals accumulate in air, soil, and water, the risk of human exposure increases among industrial workers, as well as in people living near polluted areas. Heavy metals adversely affect a variety of bodily systems such as the cardiovascular, respiratory, endocrine, immune, and reproductive systems. In addition, long-term exposure and accumulation of heavy metals in the body may disturb oxidative stress genes and thus increase the susceptibility to various diseases. Objectives. The aim of this study is to estimate the metallothionein concentration in both mussel samples from Abu Qir Bay, Egypt and the blood of local fishermen as a biomarker of exposure to metal pollution. Methods. Levels of metallothionein and heavy metals were measured in mussels. Blood levels of metallothionein and heavy metals of local fishermen were measured and compared with a control group. The effect of heavy metal exposure on oxidative stress status was investigated through the determination of malondialdehyde (MDA), catalase and glutathione content. Results. The results of this study showed high concentrations of metallothionein in mussels and in fishermen's blood, accompanied by high concentrations of metals such as cadmium (Cd), copper (Cu), lead (Pb), chromium (Cr), and zinc (Zn). At the same time, a significant decrease in glutathione content and catalase enzyme activity was associated with a significant increase in the malondialdehyde concentrations in sera of fishermen. Conclusions. The present study found that the El Maadiya region is polluted with heavy metals, inducing oxidative stress in fishermen in the vicinity. These results reveal the necessity of further environmental monitoring in the study area in order to evaluate other types of pollutants and their effects on human health.

scholarly journals Biotechnological aspects of transformation of heavy metal salts and their detoxication in milk of milk cows

2021 ◽  
Vol 262 ◽  
pp. 02010
Author(s):  
Boris Dzagurov ◽  
Oleg Getokov ◽  
Vladimir Gukezhev ◽  
Taladin Kokov ◽  
Vitaly Vorokov
Keyword(s):  
Heavy Metals ◽  
Dairy Cows ◽  
Particle Diameter ◽  
Bentonite Clay ◽  
The Body ◽  
Control Group ◽  
Milk Cows ◽  
Heavy Metal Salts ◽  
Cadmium Lead ◽  
Experimental Group

Based on the results of previous studies on pigs and poultry, in which encouraging results of a significant decrease in the concentration of heavy metals in the body were obtained, respectively, in pork, poultry and eggs, the study provided for the use of the bentonite clay of the Zamankul deposit (RNO-Alania) as an enterosorbent in relation to heavy metals and detoxification of the organism of dairy cows. In order to study the feasibility of using bentonite as an enterosorbent, studies were performed on dairy cows of the Simmental breed in the Kaloyev farm located in st. Zmeyskaya, Kirovsky district of North Ossetia-Alania. To conduct research on the principle of analog pairs, two experimental groups of cows (control and experimental, five cows in each group) of the 3rd and 4th lactation were formed. The experiment lasted for 305 days of lactation from March 2018 to January 2019. Herewith, the control group of cows was fed with a basic diet balanced in all nutritional elements, the experimental livestock, together with the main diet, daily in the composition of the concentrates was injected with crushed bentonite with a particle diameter of 4-6 mm based on the dry matter of the feed (137 g/animal). The study of the concentration of heavy metals (cadmium, lead and zinc) in soil, feed, blood and milk was performed in two periods of lactation (on the 250th and 300th days of lactation). Herewith, an increased content of heavy metals in the soil was established, relative to the MPL: cadmium - 10.1; lead - 7.4; zinc - 9.7 times more, in drinking water: cadmium - 2.5 times; lead - 9.0 times and zinc - 9.7 times more, in feed: cadmium - from 1.7 to 5.3 times; lead - from 1.1 to 1.7; zinc - from 1.0 to 2.5 times the MPL. The inclusion of bentonite to the ration of the animals of the experimental group, in comparison with the control group, in both study periods (250 and 300 days of lactation) contributed to a significant decrease in the concentration of the studied heavy metals in the blood from 17 to 20%, in milk - from 16 to 18 %. The transformation ration values of heavy metals from feed into the body, respectively, into milk, were also lower in the animals of the experimental group, relative to the control.

scholarly journals Dietary supplements based on modified and enriched with amino acids zeolite in the cultivation of young turkeys

2022 ◽  
Vol 354 (11-12) ◽  
pp. 20-23
Author(s):  
S. V. Dezhatkina ◽  
N. A. Feoktistova ◽  
E. V. Pankratova ◽  
N. A. Provorova ◽  
E. S. Salmina
Keyword(s):  
Amino Acids ◽  
Hematological Parameters ◽  
Live Weight ◽  
High Growth ◽  
Mineral Elements ◽  
High Growth Rate ◽  
The Body ◽  
Control Group ◽  
Modified Zeolite ◽  
Negative Effect

Relevance. Modern turkey crosses have a genetically determined high growth rate. These farm birds are very sensitive to even minor fluctuations in their diet, are susceptible to a deficiency of amino acids, protein, mineral elements and vitamins, which can contribute to a decrease in their productivity, the development of pathological processes and diseases. The use of modern technologies and scientific achievements has made it possible to prepare a new product — modified zeolite. This natural mineral is already free of impurities and microbes, with open pore windows, has a high ability to selectively exchange, and is obtained by various methods of activating zeolite in industrial conditions. At the same time, Ulyanovsk specialists have mastered the technology of enriching modified zeolite with useful substances.Methods. The aim of the work was to study the effect on the body of turkeys and the level of their productivity of additives based on modified zeolite enriched with amino acids. The experiment was organized for 60 days on 250 turkeys of the breed Hybrid Cread-mayker aged 55–60 days in the conditions of the Ulyanovsk region. The control group received the main ration (MR), the experimental group was given once a day in addition to the MR in the morning feeding in a mixture with mixed feed 5% additives of modified zeolite enriched with amino acids.Results. The inclusion of a supplement based on modified zeolite enriched with amino acids in the diet of turkeys does not have a negative effect on the hematological parameters of their blood, all indicators corresponded to the physiological norm. During the experiment in the 2nd group, the live weight of poultry was obtained larger by 8.70%, which amounted to 12,296 kg, respectively, more profit was obtained from the sale of turkey meat, total revenue was 133,210, and additional profit was 27,960 rubles, 2.41 rubles of profit was received for 1 ruble of costs. Тhe data indicate the normalization of mineral homeostasis in the body of turkeys and better absorption of nutrients in the feed diet.

scholarly journals The effect of Ricinus communis seeds extract on reproductive activity and blood values of male rabbits

2019 ◽  
Vol 12 (1) ◽  
pp. 97-106
Author(s):  
AL-Khafaji Nazar
Keyword(s):  
Estrogenic Activity ◽  
Ricinus Communis ◽  
Hematological Parameters ◽  
Abdominal Cavity ◽  
Hemoglobin Concentration ◽  
Reproductive Activity ◽  
Treated Group ◽  
Blood Parameters ◽  
The Body ◽  
Tropical Regions

Ricinus communis L. of Euphorbiaceae family is a widespread plant in tropical regions. It is used in traditional medicines as an anti- fertility agent in India and different parts of the world. The ether soluble portion of the methanol extract of R. communis var minor possesses anti-implantation, anti - conceptive and estrogenic activity in rats and mice when administered subcutaneously.The study was conducted on 10 local breed male rabbits, 1-2 years old, of 1-2 kg body weight. The animals were divided into two groups, control non – treated group and treated group in which animals were treated with single daily dose of 50 mg /kg b. wt. P.O. of decorticated and defatted castor seeds (DDCS) for 14 days. 28th day post treatment, animals were anesthetized by diethyl ether, sacrificed, abdominal cavity was open. The sexual organ (testes, epididymis, prostate and seminal vesical) weighed. In addition to take a biopsy from each one for histopathological changes. The study also included clinical and hematological parameters, in addition to sperm counts and the changes in sperm morphology.Body weight, body temperature increased significantly in treated males. While in non- treated group there were no significant changes. Respiratory rates and heart rate were none significantly changed in treated and non- treated males.Bleeding time none significantly increased in treated males, but increased significantly in none treated males. Clotting times decreased none significantly in treated and non- treated males. The blood parameters including, total erythrocytes count, hemoglobin concentration, PCV% , MCV, MCH, MCHC, total leucocyte and differential leucocyte counts were either increased or decreased none significantly in both groups. The results revealed that the effects of exposure to extract of ricin for 14 days on reproductive efficiency of rabbits, exhibited Significant decrease in weights of testes, epididymis, tails, heads of epididymis, seminal vesicles and prostate in treated males in comparison with those of non- treated males. While the body of epididymis did not show a significant changes.Significant decrease in live sperm numbers, number of sperms in epididymal head, in addition to deformities in high numbers of sperm, including enlarged or small sperms. breaks head, and its detachment, presence of two heads in one sperm, bifurcation of tail and its breaking, sperm coiling in samples from treated males in comparison with those from non-treated males.Histological changes were hyperplasia of lining epithelial cells and vacuolar degenerative changes, loss of spermatogenesis, and spermatocytes necrosis in those from treated males.

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