scholarly journals Dynamics of hematological parameters in rabbits for osteosubstitution by hydroxyapatite ceramics doped with germanium and in complex with blood coagulation activator

2021 ◽  
Vol 23 (102) ◽  
pp. 78-86
Author(s):  
T. P. Todosiuk ◽  
M. V. Rublenko ◽  
V. M. Vlasenko
Keyword(s):  
Blood Coagulation ◽  
Hematological Parameters ◽  
The Body ◽  
Hematological Indices ◽  
Reparative Osteogenesis ◽  
Hydroxyapatite Ceramics ◽  
Post Traumatic ◽  
Intensive Course ◽  
And Control ◽  
Experimental Group

Bone tissue has powerful regenerative properties, thanks to which, with stable fixation, quite large amounts of skeletal bone damage can be successfully repaired. However, in the case of fragmentary fractures, the use of osteosynthesis methods alone does not always ensure the optimal course of reparative regeneration, as its regenerative potential is lost. Therefore, there is a need to replace post-traumatic bone defects and stimulate reparative osteogenesis. For this purpose, doped or doped with various elements (Ge, Si, Zn, Ag, Cu) composite materials. The aim of the study was to investigate the dynamics of hematological parameters in rabbits for osteosubstitution by hydroxyapatite ceramics doped with germanium and in combination with a blood clotting activator. Model defects were formed in the radial diaphysis and femur metaphysis in rabbits with a 3 mm and 4.2 mm diameter drill bit, respectively. Anesthesia included acepromazine, thiopenate, and lidocaine infiltration anesthesia. Animals of the first experimental group (n = 12) were replaced by defects with granules of hydroxyapatite ceramics doped with germanium (HTGe), the second (n = 12) – hydroxyapatite ceramics doped with germanium with blood coagulation activator (HTGe + a), the third – (n = 12). hydroxyapatite α + β with active (α + β + a), and control – granules of undoped ceramics (HT). On day 7, animals of all groups had minor post-traumatic erythrocytopenia and oligochromemia. There was also a gradual increase in the content of leukocytes in the blood with a peak on the 14th day, which in the group HTGe + a lasted until the 30th day. The increase in the number of leukocytes and their peak values occurred within the physiological norm and only approached its upper limit. In most groups there was a pronounced thrombocytosis during the first 14 days with normalization to the 30th day, but in the 2nd experimental group the number of platelets returned to normal only on the 60th day. Changes in most integral hematological indices, which reflect the relationship between blood cell populations, are characteristic of the inflammatory-resorptive phase of reparative osteogenesis. The dynamics of hematological parameters indicates the absence of a pronounced reaction of the body to the implantation of hydroxyapatite composite doped with germanium. The combination of hydroxyapatite ceramics doped with germanium with coagulation activator eliminates post-traumatic oligochromemia and erythrocytopenia and significantly increases the level of hematological integral indices, which indicates a more intensive course of inflammatory-resorptive clinical resorptive aparation phase.

scholarly journals Radiographic, macromorphological and hematological evaluation of hydroxyapatite ceramics with diff erent physicochemical properties

2020 ◽  
pp. 140-152
Author(s):  
V. Chemerovsky
Keyword(s):  
Composite Materials ◽  
Bone Tissue ◽  
Blood Clot ◽  
Hematological Parameters ◽  
Indirect Evidence ◽  
External Jugular Vein ◽  
Control Group ◽  
Reparative Osteogenesis ◽  
Hydroxyapatite Ceramics ◽  
Experimental Group

Treatments of fragmentary fractures in case of loss regenerative potential of bone tissue require usingdiff erent composite materials. Among all of them, ceramics based on synthetic hydroxyapatite and tricalcium phosphate are considered promising. The reparative osteogenesis in spongy and compact rabbit’s bone tissueusing 3 composite materials with diff erent physicochemical propertieswas studied. A reparative osteogenesiscontrolled by monitoring clinical, radiologic and macromorphologic parameters. Composite materials infl uence on the organism was studied by determining the dynamics of hematological parameters throughout the study period. To realize this scheme was formed a 3 experimental groups and one control group of rabbits. The created defects of animals of fi rst experimental group were fi lled out with GT + α-TKF-500, thedefects of second oneanimals group fi lled out with GTlKl-2, and defects ofthird oneanimals group fi lled out with GTlKg-700. The GTlKl-700materials were doped with silicon. In control animals, bone defects were allowed to heal under a blood clot. During the studies, all animals were located in the same environment conditions. A blood samples for hematological investigation was taken from the external jugular vein before anesthesia and at the 3rd, 7th, 14th, 21st and 42nd day after surgery. X-ray examination was performed on 14th, 21st, and 42nddays.The animals were removed from the experiment on 21stand 42nd days. The samples were examined by macromorphologic method. By radiologic examination was found that composite materials had osteoconductive properties except the silicondoped example which hadosteoinductive properties. The samples of compact bone tissue of 1st and 3rd experimental groups characterized by formation of punctate osteosclerosis with a compacted contour of the periosteumon the 42nd day. But the bone samples of 2nd experimental group had compacted contours of the periosteum only. However, in the cancellous bone tissue on the 42nd day in the animals of the 1st experimental group developed point osteosclerosis, which visualized as individual granules of the composite.But in the animals of 2nd group thewhite spot which was outlined shape and homogeneous structure at the defect was found. The bone samples of 3rd animals group shoved a clearly limited point osteosclerosisforming were was found ceramic granules. In case using ofGT + α-TKF-500, a strong connection of granules from the formed bone tissue and without any periosteal growthswas noted. Using GTlKl-2 places of defects were identical to not injured sites of radial bones. Using GTlKg-700 shows that bone defect is fi lled to the level of the plane of the bone surface and covered with a periosteum without visible growths. The granules of the composite are evenly distributed in the regenerate and associated with bone tissue. The analysis of hematological parameters did not reveal any fundamental diff erences, but the use of hydroxyapatite implants is not accompanied, in contrast to spontaneous reparative osteogenesis, the development of leukocytosis, which indicates a moderate course of its infl ammatory reaction. But the latter is accompanied by a platelet reaction, the most pronounced when using implants, which is probably due to the infl uence of platelet factors and is indirect evidence of early osteoblastic reaction. Key words: fractures, rabbits, erythrocytes, leukocytes, platelets, hemoglobin.

scholarly journals Експериментальне зараження поросят вірусом епідемічної діареї свиней

2017 ◽  
Vol 19 (77) ◽  
pp. 208-213
Author(s):  
D. Masiuk ◽  
A. Sosnitskiy ◽  
A. Kokarev ◽  
S. Koliada
Keyword(s):  
Real Time ◽  
The Body ◽  
Control Group ◽  
Rt Pcr ◽  
Internal Organs ◽  
Real Time System ◽  
E Coli ◽  
Neonatal Piglets ◽  
And Control ◽  
Experimental Group

There were infected neonatal piglets in the first days of their lives PED virus suspension derived from pigs previously PED patients. Diagnosis for PED in piglets donor virus PED was inserted complex method for clinical and epizootic performance and confirmed the identification PEDV by PCR-RT using the test system «EZ-RED/TGE/PDCoV MPX 1.0 Real time RT-PCR» company Tetracore (USA) Thermocyclers CFX 96 Real-Time System company BIO RAD (USA). Homogenate small intestine of pigs PEDV donor, prepared in a blender for PCR in a thick band of 18 animal carcasses, frozen at -18 °C without cryopreservation and kept 359 days. Before infecting pigs and strip defrost by RT-PCR identified the concentration of the virus genome equivalents (GE) without establishing viable virions quantitative pathogen. For Sample 20 selected analog neonatal piglets, divided them into 3 experimental groups (group 1 – 5 piglets, group 2 – 5 piglets and group 3 – 7 piglets) and one control (3 piglets). Research pigs infected per os virus-containing suspension with a concentration PEDV 1.03×106 GE/cm3. The dose for infection first group was 6 cm3 (6.18×106 GE/cm3), for the second – 5 cm3 (5,15 × 106 GE/cm3), for the third – 4 cm3 (4.12 GE×106/cm3) homogenate. The fourth group – control (not infected). All the pigs were in identical conditions that fully meet the physiological needs of the body. Of the 17 infected pigs only 2 was infected PEDV. PED was confirmed by laboratory methods. In bacteriological examination of internal organs of pigs that came out of a research experiment and control group were diagnosed colibacteriosis. In the control group was isolated from heart and intestinal non-pathogenic for white mice E. coli. From pigs 1 and 2 research groups has been allocated to white mice nonpathogenic E. coli, is set colibacteriosis; 2 experimental group found in one pig hemolytic E. coli; 3 experimental group from the internal organs of pigs in conjunction with non-pathogenic for mice intestinal former cane isolated Klesiella spp., is diagnosed with mixed infection (E. coli, Klesiella spp.). From the intestine of experimental and control pigs do not identified beneficial microflora – aerococcus, lactobacteria, bifidobacteria and cultured putrefactive anaerobic spore facultative and non spore microflora.

scholarly journals The needs and characteristics of feeding dairy cows

2017 ◽  
Vol 19 (79) ◽  
Author(s):  
L. G. Levitskaya
Keyword(s):  
Dairy Cows ◽  
Milk Yield ◽  
Positive Impact ◽  
The Body ◽  
Control Group ◽  
Milk Productivity ◽  
Feed Management ◽  
Feed Costs ◽  
Intensive Course ◽  
Experimental Group

The influence of feed with different contents in them in certain ratios lekarstvennogo and insoluble protein on the body of dairy cows. In particular, digestion in the rumen, digestibility of nutrients of feed, milk productivity and milk quality. Identify the differences in some indicators of digestion in the rumen of cows, the digestion of protein (carrying experience) on the background of the use of the experimental feed. In this grain component was subjected to extrusion and introduced maize, the protein which vikoristannya. Because of this, reduced the solubility of protein from 38% to 29% that is 9%. As a result, extended the time of their digestion in the rumen. So scar microflora sufficiently transformed the ammonia available for absorption of microbial protein, which has a positive impact on the processes of digestion and assimilation of nutrients and contributed to improving milk productivity in cows. The structure of the diets of both groups was identical and included: roughage is 12.5% juicy – 75.0%, concentrated to 12.5%. One fodder unit in the control group had 90.2 g digestible protein in experimental 89.6 g Sharapodinov attitude in control and experimental diets is 0,98. The ratio of mineral substances: CA : P in the control and experience is 1.6 : 1, N : S respectively of 7.3 and 6.9. On a more intensive course of metabolic processes show physiological and biochemical parameters in animals of the experimental group. Namely: reducing the amount of ammonia and the increase in the number of bacteria in the rumen. The composition of basic ration (PR), which included feed management. All experimental animals during the preparatory period received the same diet. Research in accounting period II (experienced) group – experimental mixed fodder in which to reduce the solubility of the protein component of grain (oats, barley, wheat) extrudable and additionally introduced maize grain (15%), the control group received commercial feed. Found that feeding the experimental feed, improved nitrogen metabolism in the organism of cows, heifers, which ultimately increased milk yield by 4.4% (vs. 26.3 to 25.2 kg). The reduction in feed costs and increase milk yield in cows of the experimental group resulted in a reduction of cost of 1 quintal of milk 27.1 UAH. and improve profitability by 2.3% against the control.

scholarly journals CONVERGES OF SKY YOGA AND KAYAKALPA ON THE CORE MUSCLE STRENGTH AND AGEING FACTOR

2019 ◽  
pp. 1-2
Author(s):  
K. Krishnaveni ◽  
M. Jothilakshmi*
Keyword(s):  
The Body ◽  
Test Method ◽  
Control Group ◽  
Mental Ability ◽  
Not Given ◽  
The Core ◽  
Group I ◽  
And Control ◽  
Experimental Group ◽  
Neurological System

INTRO: Today's generation children are growing faster in all aspects mentally,physiologically and physically too than before generation.The life span of the human being got reduced to averagely 63 age,value tells more than the words and What will be the future? Are the children are not going to see their grandfather and grandmother? These are the question which lead this study to overtaken. METHOD:The college boys from in and around Coimbatore were selected as subjects for the propose of study.The total 60 subjects were selected and they age in 18 to 21 years.Then subjects were divided into three groups,each containing 20 members.Experimental Group I given SKY yoga with Kayakalpa training and Experimental Group II were given only SKY yoga training for 5 days in week for 10 weeks and control group not given any training. RESULTS:The study shown that there was improvement in the coordination of hand- mind and ability to think measured by Shuttle cross pick-up test method to the Experimental groups I & II than the subjects in control group. The coordination of hand- mind has increased this shown that SKY yoga effects the neurological system and mental ability of the body. CONCLUSION: The result shown that SKY Yoga with Kayakalpa on college students has positively impact on the core muscles and ageing factor

Hematological Indicators of Moose in Domestication Process

2020 ◽  
Keyword(s):  
Hematological Parameters ◽  
Physiological State ◽  
Blood Parameters ◽  
The Body ◽  
Sex Characteristics ◽  
Hematological Indices ◽  
Blood Indices ◽  
Hematological Indicators ◽  
Age And Sex ◽  
Selection Of

Presented hematological indices for a stress-free selection of material from moose in the process of domestication depending on age and sex characteristics. Fluctuations in the blood parameters of moose have been established within various limits, taking into account the influence of various factors - the climatic, environmental, technological, physiological state of the animal, and age and sex characteristics. Considerable attention is paid to the selection of blood from moose, one of the most labor-intensive processes when working with them. Some tendency to fluctuations in hematological parameters in young moose has been recorded. This, possibly, is associated with a decrease in the action of colostral immunity, the formation of a specific defense of the body. Differences in blood indices in moose depending on gender were unreliable. However, in males, some of them - in terms of the content of erythrocytes, basophils, and monocytes - was higher than in females, and vice versa, in females - the number of leukocytes, lymphocytes, and eosinophils. It was shown that in young animals of 21-50 days of age, a neutrophilic profile prevails with increased content of segmented forms, and in adults - a lymphocytic profile. Compared with moose, which are in the process of domestication in animals living in natural conditions, expressed leukocytosis was recorded. As a result of the studies, the estimated norms of morphological parameters of the blood of moose in the process of domestication are established, depending on age and gender.

Evaluation of the effectiveness of ironcontaining drugs in the complex therapy of antenatal hypotrophy of piglets

2021 ◽  
Vol 1 (6) ◽  
pp. 13-17
Author(s):  
R. V. Rogov ◽  
◽  
Zh. Yu. Muradyan ◽  
I. G. Ryazanov ◽  
◽  
...  
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Clinical Study ◽  
Body Weight Gain ◽  
Hematological Parameters ◽  
Live Weight ◽  
The Body ◽  
Treatment Regimens ◽  
Complex Therapy ◽  
Experimental Group

In the conditions of the pig breeding complex, the effectiveness of two treatment regimens for antenatal hypotrophy of piglets was studied. Two groups of piglets were formed, where the ironcontaining drug «Ursoferran – 200» was used in complex therapy in the 1st experimental group, and «Ferropen-200»in the 2nd experimental group. In a clinical study, hypotrophic piglets showed characteristic symptoms of the disease. In the experiment, the live weight of piglets was measured at the time of farrowing and on the 3rd, 7th, 14th and 21st days of life. The hematological parameters were studied. The most pronounced therapeutic effect was observed in the 1st experimental group, where the body weight gain was higher by 8,3% than in the 2nd experimental group.

DYNAMICS OF PROTEIN METABOLISM AND ACTIVITY OF AMINOTRANSFERASES OF DOGS BY ADDING DIHYDROQUERCETIN

2016 ◽  
Vol 1 (2) ◽  
pp. 60-63
Author(s):  
Полищук ◽  
Sergey Polishchuk ◽  
Молянова ◽  
Galina Molyanova
Keyword(s):  
Body Weight ◽  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
The Body ◽  
German Shepherd ◽  
Physiological Indicators ◽  
Formalin Edema ◽  
And Function ◽  
Experimental Group

The purpose of research is to increase protective and adaptive reactions, the service and the working potential of an organism of dogs through the use of DHQ. The article presents the results of an experiment to the effect of DHQ on the dynamics of hematological parameters of dogs blood. Dihydroquercetin – an active antioxidant, a natural scavenger of oxygen free radicals, hepatoprotective, has anti-inflammatory action due to limitations of the formalin edema and histamine, and inhibits the formation of serous fluid, pain killers, immunocorrectional properties. Due to the high complexing properties it displays the body of heavy metals, including radionuclides, it helps to restore the tone of blood vessels, normalization of the lipid spectrum of the blood and slows the development of atherosclerotic plaques. Investigations were carried out in Dogs State Service Ministry of I A of Russia zonal center in Samara Region by clinically healthy of dog breed German Shepherd, age 2-4 years with an average body weight of 30 kg on the background conditions and feeding, adopted by the enterprise. Dogs experimental group received Dihydroquercetin to 0.001 g/kg body weight dose once a day with food. When added to the basic diet Dihydroquercetin dog experimental group show an increase in indicators such as red blood cells – by 18.3% (p<0.01), hemoglobin – by 11.7% (p <0.01) hematocrit – 7.1% (p<0.01), white blood cells – by 9.1% (p<0.05) compared to control data. The research results give reason to believe that the use of dietary supplements in Dihydroquercetin in dogs diet dose 0.001 g/kg allows significantly increase the morphological and physiological indicators of animal organism. In this case a significant increase erythrocytes and hemoglobin and hematocrit indicates increased oxidative metabolism and function of the intensity of the blood in dogs treated with the drug, and increase in the blood indicates an increase in immune status.

The Comparison on Changes of the Body Heats in Electroacupuncture Analgesia and Anesthesia by Ketamine Hydrochloride in Dogs

2006 ◽  
Vol 34 (01) ◽  
pp. 69-76 ◽  
Author(s):  
Sang-Eun Lee ◽  
Ji-Min Seo ◽  
Jianzhu Liu ◽  
Min-Sung Hong ◽  
Young-Won Lee ◽  
...  
Keyword(s):  
The Body ◽  
Control Group ◽  
Control Groups ◽  
Significant Difference ◽  
Ketamine Hydrochloride ◽  
Ketamine Anesthesia ◽  
Healthy Dogs ◽  
And Control ◽  
Experimental Group ◽  
Body Heat

This study was performed to clarify the differences of the body heats between electroacupuncture analgesia (EA) and anesthesia by ketamine hydrochloride (ketamine anesthesia) in dogs. Nine clinically healthy dogs were divided into ketamine anesthesia (control: 5 heads) and EA (experimental: 4 heads) groups, respectively. The acupoints GV-5 and Bai-Hui were used. The infrared thermographic system was used to determine the body heats. The body heats was determined at areas such as the dorsocranial (DCr), dorsocaudal (DCd), ventrocranial (VCr) and ventrocaudal (VCd) regions, on pretreatment, 10, 20, 30, 50 and 90 minutes after treatments, respectively in control and experimental groups. The body heats showed decreasing tendency until 30 minutes after ketamine injection, and then showed increasing pattern until 90 minutes after at all areas investigated in the control group. However, no significant differences of the body heats in the DCr, DCd, VCr and VCd regions were found in the control group. On the other hand, the body heats showed increasing tendency until 30 minutes, and then showed decreasing pattern until 90 minutes after EA, in the experimental group. The significant difference was observed at 30 minutes in the DCr region, and also at 10, 20 and 30 minutes in the DCd regions in the experimental group ( p < 0.05). The significant differences of the body heats were detected at 20 minutes in the DCr region, at 30 minutes in the DCd region and at 30 minutes in the VCd region between the experimental and control groups ( p < 0.05). In conclusion, EA increases of the body heat in the contrary to that of ketamine anesthesia.

scholarly journals Evaluation of Sperm Impairing Factor from Serratia marcescens as Male Contraceptive in Mouse Model

2019 ◽  
Vol 2019 ◽  
pp. 1-9
Author(s):  
Aditi Chauhan ◽  
Vijay Prabha
Keyword(s):  
Body Weight ◽  
Serum Level ◽  
Serratia Marcescens ◽  
Hematological Parameters ◽  
The Body ◽  
Saline Control ◽  
Seminiferous Tubules ◽  
Hematological Indices ◽  
Seminal Parameters ◽  
The Right

The present study was carried out to assess the contraceptive efficacy of sperm agglutinating factor (SAF) isolated from Serratia marcescens, in male Balb/c mice. Mice were administered via an intratesticular route with different concentrations of SAF, viz., 10, 50, 100, 200, or 400 µg, in the right testis only which served as a test while the left side served as control except otherwise stated. Mice were sacrificed on day 3, 7, 14, 21, 30, 45, 60, and 90 after administration, and results in terms of change in body weight, seminal parameters, tissue somatic indices (TSI), hematological parameters, serum level of testosterone, lipid peroxidation, and histology were studied. The body weight and TSI remained unaffected in all the experimental groups. In case of seminal parameters, the right testis treated with 10 μg, 50 μg, 100 μg, 200 μg, or 400 μg of SAF showed azoospermia up to day 7, 14, 21, 45, and 90, respectively. The hematological indices, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) were found to be unaltered when the group receiving SAF (test) was compared with the groups receiving phosphate buffer saline (control) in the right testis; however, the treatment had a negative effect on the serum level of testosterone. It also affected the oxidative status of the right testis. Furthermore, histological studies revealed hypospermatogenesis and alterations in the seminiferous tubules which included intraepithelial vacuolation and exfoliation in the right side as compared to the left side. Thus, the results suggest that SAF (400 µg) causes suppression of spermatogenesis, without causing apparent toxic effects.

scholarly journals Effects of Diversified Sports Activity Module on Physical Fitness and Mental Health of 4–5-Year-Old Preschoolers

2021 ◽  
Author(s):  
Jinfu Liu ◽  
Feng Gao ◽  
Lugang Yuan
Keyword(s):  
Mental Health ◽  
Physical Fitness ◽  
Body Shape ◽  
The Body ◽  
Control Group ◽  
Sports Activity ◽  
Standing Long Jump ◽  
Long Jump ◽  
And Control ◽  
Experimental Group

Background: Chinese children are poorer in sports activity as compared to foreign children and their prospects as to physical fitness are not optimistic. This study aimed to discuss the effects of diversified sports activity modules on physical fitness and mental health of preschoolers ages 4–5 years. Methods: Sixty preschoolers aged 4–5-yr-old from two kindergartens in Jiaxing of China were selected randomly during Mar-Oct 2019. These respondents were divided into the experimental group (n=30) and the control group (n=30). The experimental group implemented the diversified sports activity module for 16 wk successively, 5 d per week, and 30-45 min per day. The control group adopted conventional sports activity. The body shape, physical fitness, and mental health of the two groups were measured and compared. Results: For body shape, the height of the experimental group increased is significantly higher than that of the control group. The experimental and control groups show no significant differences in body weight. For physical fitness, the experimental group is significantly superior to the control group in testing results of standing long jump, double-feet continuous jump, balancing on one foot, tennis shot, sit-and-reach, and 10 m repeated run. For mental health, the testing results of action development, language development, and social development of the experimental group are significantly better than those of the control group. Conclusion: The diversified sports activity module is not only conducive to strengthening the physical fitness of 4–5-year-old preschoolers but also improves their mental health.

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