scholarly journals Age dynamics of hematological indices in dry cows

2020 ◽  
Vol 22 (98) ◽  
pp. 42-45
Author(s):  
L. V. Koreyba ◽  
Y. V. Duda

The requirement for the study of blood parameters is determined by its physiological significance and changes that occur in different physiological and pathological conditions. The hematological profile is an important criterion that shows the physiological condition of animals. The physiological changes of blood in cows have been established concerning the conditions of keeping and feeding, hygienic parameters, in the dynamics of the dry season, in different seasons of the year, etcetera. We have not found any information on studies of the dynamics of blood parameters in pregnant animals, depending on their age, in the study of literary sources in recent years. The purpose of our work was to study the age-related dynamics of hematological blood parameters in cows during the dry period. Our studies aimed to determine the hematological indices of Holstein breed cows in the physiological course of the prenatal period during the dry period, depending on the number of deep calving. Control and experimental groups were formed on the principle of group-analogues (by breed, age, sex, physiological state, weight, milk productivity). Animals kept in typical rooms with satisfactory microclimate parameters (in winter) and at camps (in summer). Laboratory studies were carried out in the laboratory of the Dnipro State Agrarian and Economic University. Hematologic studies were performed according to the following indicators: erythrocytes, hemoglobin, color index, erythrocyte sedimentation rate, leukocytes, monocytes, lymphocytes, neutrophils with rod-like shape and segmented nucleus, basophils and eosinophils according to conventional methods. The results of our studies indicate that the lowest number of erythrocytes was detected in the cows of the sixth calving and the highest hemoglobin content, which led to the rise of the color index – in cows of the fifth pregnancy present the reverse tendency; increase to the maximum values of the number of leukocytes in cows of the third body (1.32 times), which is a reflection of the greater reactivity of the body of cows of this age group; a probable decrease (by 10.59 %, P < 0.05) of lymphocyte counts was found in sixth-calf cows, indicating a weakened immune response; also increased eosinophils almost 1.75 times (P < 0.05) compared to fifth pregnancy; the trend of a gradual decrease in the number of segmented neutrophils in calving cows is followed by a second to fifth pregnancy and a significant reduction in the number of stab neutrophils in cows of the fifth pregnancy of up to 1.33 %.

Presented hematological indices for a stress-free selection of material from moose in the process of domestication depending on age and sex characteristics. Fluctuations in the blood parameters of moose have been established within various limits, taking into account the influence of various factors - the climatic, environmental, technological, physiological state of the animal, and age and sex characteristics. Considerable attention is paid to the selection of blood from moose, one of the most labor-intensive processes when working with them. Some tendency to fluctuations in hematological parameters in young moose has been recorded. This, possibly, is associated with a decrease in the action of colostral immunity, the formation of a specific defense of the body. Differences in blood indices in moose depending on gender were unreliable. However, in males, some of them - in terms of the content of erythrocytes, basophils, and monocytes - was higher than in females, and vice versa, in females - the number of leukocytes, lymphocytes, and eosinophils. It was shown that in young animals of 21-50 days of age, a neutrophilic profile prevails with increased content of segmented forms, and in adults - a lymphocytic profile. Compared with moose, which are in the process of domestication in animals living in natural conditions, expressed leukocytosis was recorded. As a result of the studies, the estimated norms of morphological parameters of the blood of moose in the process of domestication are established, depending on age and gender.


Author(s):  
S. S. Mirgalimova ◽  
A. O. Trufanova ◽  
R. R. Fatkullin

The physiological state of animals is to a certain extent characterized by hematological indicators, since blood occupies a special place in the body. By transferring nutrients and biologically active substances, the blood performs the general regulation of vital functions of the body. The use of Vitartil as a feed additive in the diets of animals leads to an increase in glycolytic processes and a decrease in the body’s need for oxygen delivery, which contributes to the growth of the animal body. The purpose of the research was to study the changes in blood parameters taking into account the age of Hereford steers against the background of the use of Vitartil feed additive to the main diet and its effect on the productive traits of animals. In order to carry out the experiment two groups of steers-analogs of Hereford breed have been formed at the age of 6 months per 10 heads in each. The difference between the groups was that the steers of the experimental group have been received the feed additive Vitartil in addition to the main diet. The greater yield of the meat part was in the steers of the experimental group in comparison with the herdmates of the control group. This indicator in the experimental group was 78,9 %, while in the control group it was lower by 1,6 %. The animals of the experimental group were superior to their herdmates in terms of meat weight by 1,4 kg or by 8,8 %. The relative number of bones was lower in the experimental group and amounted to 18,4 %, and in the control group 19,2 %. The meat content index was high in the steer have been received the biologically active additive Vitartil and was 4,3, and in the control group 4,0. It has been found that the use of Vitartil feed additive to the main diet of Hereford steers increases the blood content of hemoglobin and glucose, which has a certain effect on the morphological and chemical composition of meat, as well as increases its energy value.


2021 ◽  
Vol 36 ◽  
pp. 06014
Author(s):  
Zh.Kh. Kurashev ◽  
V.M. Gukezhev ◽  
M.Kh. Zhekamukhov ◽  
V.Kh. Vorokov ◽  
Z.S. Shibzukhov

The article presents a comparative assessment of the dynamics of biochemical and hematological parameters of the blood of the black-and-white Holstein breed of the Dutch breeding in the conditions of a robotic farm for the first two lactation to establish the influence of climatic conditions on the adaptation process. It was found that, with the exception of the hemoglobin concentration (HGB), increases in the level of their content were noted for all other analyzed blood parameters, which indicates a different degree of overload experienced by animals in conditions of a sharp change in climatic conditions. It is quite natural that the climate, the feeding conditions, and the maintenance (composition and structure of the diet), although the level of feeding is quite high, cannot but affect the physiological state of the animals to varying degrees, which must be considered during operation.


Author(s):  
А.В. МАТВЕЕВА ◽  
Э.О. САЙТХАНОВ

Цель исследований заключалось в изучении клинико-физиологического состояния и оценке интенсивности процессов перекисного окисления липидов у коров с патологиями конечностей в сравнении со здоровыми. Работа проводилась в СПК «Вышгородский» Рязанского района Рязанской области, мы проводили физикальное обследование для определения клинически здоровых коров и коров с заболеваниями конечностей. В контрольную и опытную группы включались коровы голштинской породы, массой 550-650 кг, 3-4-й лактации. В опытную группу 2 включались коровы с заболеваниями конечностей: язвы Рустергольца, абсцесса венчика, пальцевого пододерматита, гнойного пододерматита. Определяли продукты перекисного окисления: количество диеновых конъюгатов, малонового диальдегида, кетодиенов; определяли содержание холестерина в сыворотке крови. У коров с заболеваниями конечностей и находящихся в стрессовом состоянии в крови повышаются глюкокортикостеройды – гормон кортизол, вследствие этого запускается процесс перекисного окисления липидов (ПОЛ), что приводит к деградации гормонов стресса, и организм начинает усилено отвечать кортикотропной функцией гипофиза. В процессе перекисного окисления липидов накапливается значительное количество продуктов ПОЛ, у коров нарушается процесс глюкогенеза, нарушается гомеостаз. При патологических процессах, а именно при патологиях конечностей, возникает окислительный стресс в организме коров, который сопровождается процессами перекисного окисления липидов, что сопровождается повышением в крови количества конъюгированных диенов, кетодиенов, малонового диальдегида; на фоне повышения продуктов перекисного окисления снижается содержание холестерина у больных коров. При окислительном стрессе вследствие возникших патологических процессов конечностей у коров происходит изменение клинико-физиологических показателей: снижение активности, аппетита, реакции на внешние раздражители; повышается температура тела, частота пульса. The aim of the research was to study the clinical and physiological state and assess the intensity of lipid peroxidation processes in cows with limb pathologies in comparison with healthy ones.The work was carried out in the farm of the Ryazan region in the SEC "Vyshgorodsky" of the Ryazan region, we conducted a physical examination to determine clinically healthy cows and diseases of the limbs. The control and experimental groups included cows of the Holstein breed, weighing 550-650 kg, 3-4 lactations. The experimental group N2 included cows with diseases of the extremities: Rustergolts ulcer, corolla abscess, digital subdermatitis, purulent subdermatitis. Peroxidation products were determined, namely: the amount of diene conjugates, malondialdehyde, ketodienes, and serum cholesterol was also determined. Glucocorticosteroids, namely the cortisol hormone, increase in cows with limb diseases and in a stressed state, namely, the lipid peroxidation process, which leads to the degradation of stress hormones and the body begins to respond more intensely to the corticotropic function of the pituitary gland. In the process of lipid peroxidation, a signifi cant amount of lipid peroxidation products is accumulated, the process of glucogenesis is disrupted in cows, and homeostasis is disrupted. In pathological processes, namely in pathologies of the extremities, oxidative stress arises in the body of cows, which is accompanied by lipid peroxidation (LPO) processes, which is accompanied by an increase in the number of conjugated dienes, ketodienes, malondialdehyde in the blood, while cholesterol content decreases in the presence of increased peroxidation products sick cows. With oxidative stress due to the arising pathological processes of the limbs in cows, there is a change in clinical and physiological parameters, there is a decrease: activity of cows, appetite, reaction to external stimuli; rises: body temperature, pulse rate.


2017 ◽  
Vol 19 (74) ◽  
pp. 24-28
Author(s):  
N. I. Hradovych

Many authors noticed the actuality of fish hematological parameters usage as indices of anthropogenic influence. Blood system reflects the reaction of the body on the action of different ecological factors. The problem of heavy metals influence on hematological parameters of silver cap (Hypopthalmichtys molitrix) needs to be investigated. The aim of the work was studying of ecotoxical Plumbum and Cadmium influence on hematological indices of silver carp (Hypopthalmichtys molitrix) during the period exposition. The results of the investigations concerning the influence of Plumbum and Cadmium on some hematological indices of silver carp (Hypopthalmichtys molitrix) for 12 days exposition up to the action of these heavy metals are given in this article. Experimental part was realized at the Lviv research station of Institute of fisheries NAAS (Lviv oblast, Horodok region, Velykyi Lyubin). Experimental groups contained 10 two-years individuals each. Fish were adopted to laboratory conditions for 5 days under the temperature 19 ± 2ºС, рН = 7.3 ± 0.2, oxygen content 7–8 mg/l. Blood parameters were measured on the sixth and twelfth days at the control group and groups which were under the influence on the level of 2 and 5 MPC of the appropriate metal. It was proved that the influence of Plumbum and Cadmium causes the lowering of erythrocytes number, hemoglobin and corpuscular volume: the effect becomes stronger when he concentration increases and in time. The effect from intoxication by Cadmium comes faster than by Plumbum.


Author(s):  
V. A. Utkina ◽  
◽  
L. M. Stepchenko ◽  

The research objective is to establish Hyplus meat breed of young rabbits and to determine growth and development characteristics during the period from weaning to slaughter age under conditions of the biologically active feed additive of humic nature «Humilid» introducted into their general diet. The control and experimental groups of animals were formed according to the principle of similar groups. Experiment study lasted for 35 days, including 7 days preparatory period, 21 days research period and 7 days aftereffect period. The young rabbits of the control and experimental groups were fed with the main diet, which included compound feed with an individual calculation of nutrients per animal. The young rabbits of the experimental group were additionally injected with a biologically active feed additive «Humilid» in the form of an aqueous solution in an amount of 5 mg/kg of body weight according to the active substance to the main diet for 21 days. During the experiment, the effect of «Humilid» on the growth and development of young rabbits and their physiological state was studied. The weighing of young rabbits in the experimental groups was carried out once a week. At the same time, their body weight was determined on average and average daily growth, the safety of the livestock, in addition, the weight and mass index of the liver, heart and kidneys after slaughter by the calculation method. With the introduction of the feed additive «Humilid» to young rabbits, an increase in the body weight of the animals is observed throughout the experiment. The body weight on average of young animals of the experimental group exceeded this indicator in comparison with animals in the control group by 17.2 % (p˂0.001). Under the conditions of the introduction of the biologically active feed additive «Humilid» over the period of the experiment, the average daily weight gain of young rabbits in the experimental group was higher by 20.7 % (p˂0.01) compared to this indicator in the animals of the control group, respectively. The safety of the livestock was 100% in both groups. However, it should be noted that young rabbits of the control group had more pronounced motor activity and signs of anxiety than the animals of the experimental group, which obtained the feed additive of humic nature «Humilid». When «Humilid» was used in the diet, the mass of organs and the index of their development in young rabbits in the experimental and control groups of animals were within the range of age-related changes, which indicates the non-toxic effect of this additive.


Author(s):  
A. E. Chernikova ◽  
Yu. P. Potekhina

Introduction. An osteopathic examination determines the rate, the amplitude and the strength of the main rhythms (cardiac, respiratory and cranial). However, there are relatively few studies in the available literature dedicated to the influence of osteopathic correction (OC) on the characteristics of these rhythms.Goal of research — to study the influence of OC on the rate characteristics of various rhythms of the human body.Materials and methods. 88 adult osteopathic patients aged from 18 to 81 years were examined, among them 30 men and 58 women. All patients received general osteopathic examination. The rate of the cranial rhythm (RCR), respiratory rate (RR) heart rate (HR), the mobility of the nervous processes (MNP) and the connective tissue mobility (CTM) were assessed before and after the OC session.Results. Since age varied greatly in the examined group, a correlation analysis of age-related changes of the assessed rhythms was carried out. Only the CTM correlated with age (r=–0,28; p<0,05) in a statistically significant way. The rank dispersion analysis of Kruskal–Wallis also showed statistically significant difference in this indicator in different age groups (p=0,043). With the increase of years, the CTM decreases gradually. After the OC, the CTM, increased in a statistically significant way (p<0,0001). The RCR varied from 5 to 12 cycles/min in the examined group, which corresponded to the norm. After the OC, the RCR has increased in a statistically significant way (p<0,0001), the MNP has also increased (p<0,0001). The initial heart rate in the subjects varied from 56 to 94 beats/min, and in 15 % it exceeded the norm. After the OC the heart rate corresponded to the norm in all patients. The heart rate and the respiratory rate significantly decreased after the OC (р<0,0001).Conclusion. The described biorhythm changes after the OC session may be indicative of the improvement of the nervous regulation, of the normalization of the autonomic balance, of the improvement of the biomechanical properties of body tissues and of the increase of their mobility. The assessed parameters can be measured quickly without any additional equipment and can be used in order to study the results of the OC.


2014 ◽  
Vol 1 (1) ◽  
pp. 42-46
Author(s):  
L. Yuskiv ◽  
V. Vlizlo

Aim. To investigate the vitamin D status in highly productive cows during winter housing period and effect of cholecalciferol by various ways of vitamin D 3 injection to cows in last days of gestation and after calving. Methods. Enzyme-linked immunoassay, spectrophotometry. Results. It has been stated that intramuscular injection of cholecalciferol into cows caused increase of the vitamin D 3 active metabolite – 25-OHD 3 , calcium, phosphorus and magnesium levels together with decrease of alkaline phosphatase level in pre- and post-natal periods. Oral supplementation makes little infl uence on the studied blood parameters of cows. Conclusions. Extrabuccal administration and oral supplementation of cholecalciferol in winter housing period to high-yield cows in the last days of gestation and after calving is accompanied by increased levels of its metabolites and their effect on mineral metabolism in the postnatal period. The nature of these changes depends on the mode of vitamin D administration and the physiological state of the cows.


Author(s):  
N. I. Maslova

The article presents analysis of material and results of their own studies on changes in the permeability of cellular structures, organs and tissues in carp, which is of great importance in determining age-related indicators. The cells permeability in liver and gonads estimation was carried out under the experimental base of VNIIR on two carp genotypes during the pre-spawning period. The carp groups taken for analysis differed significantly in their genotypes. In females of the Khrapunov group the fecundity was 2023.0 thousand units, while the number of oocytes filled with yolk was only 0.7%, in the Ostashevsky ones - 1370.0 thousand units and 8.6%, respectively. During estimation the chemical composition of the generative tissue in females and males it was established that the cholesterol and lecithin content in males is higher than that of females, while feeding dependence is observed, especially on the amount of protein in the diet. For example, in females on protein diet contained less glycogen in gonads than on females on carbohydrate diet. Lecithin and cholesterol are higher in males than in females, which corresponds to increasing the Gyurdy Ratio (estimation of cell membrane strength). In spermatogenesis the content of phospholipids and cholesterol in the liver was decreased less than during ovogenesis. This indicates a lower level of synthetic processes in the milts compared with the ovaries. The cholesterol content in sperm is higher than in caviar in 19.6 times, and phospholipids almost doubled. With increasing age, the Gyordy Ratio for caviar decreases, for sperm it increases, the percentage of caviar fertilization increases. As the body age metabolism deteriorates, cellular permeability decreases (the ratio of lecithin and cholesterol changes significantly). At the same time, the permeability of cells in different organs and tissues varies and depends on living conditions, especially feeding and to some extent on the origin. In fish the gross productivity decreases as growth slows down and more energy is spent on adaptation to environmental conditions.


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