scholarly journals Effects of Imidacloprid-Contaminated Feed Exposure on Hematological Parameters in Adult Rabbits (Oryctolagus Cuniculus)

2020 ◽  
Vol 7 (3) ◽  
pp. 439-444
Author(s):  
Md Alamgir Kobir ◽  
Latifa Akther ◽  
Imam Hasan ◽  
Md Ahosanul Haque Shahid ◽  
Ziaul Haque ◽  
...  
Keyword(s):  
Leukocyte Count ◽  
Hematological Parameters ◽  
Blood Parameters ◽  
The Body ◽  
Standard Diet ◽  
Cell Analysis ◽  
Differential Leukocyte Count ◽  
Once Daily ◽  
Important Index ◽  
Fresh Vegetables

Blood parameters are an important index to assess the body homeostasis in mammals. Here, the effect of pesticide (imidacloprid) contaminated feed exposure on the haematological parameters of adult rabbits (n=15) was studied. Pesticides (Imidacloprid, Bildor® 0.5ml (100mg)/L water spray on vegetables and green grass) exposed feed fed to rabbits with wash (washed feed rabbit) or without a wash (not washed feed rabbit) in the once-daily morning for every alternative day up to 15 days. Control rabbits have received a standard diet (fresh vegetables and green grass). The blood cell analysis showed that the total erythrocyte count, packed cell volume, and haemoglobin values were not changed significantly in pesticide exposed rabbit. For differential leukocyte count, the percentage of neutrophil and eosinophil was significantly decreased in the pesticide-exposed rabbit as compared to the control rabbit. The lymphocyte percentage was increased significantly in pesticide-exposed rabbits compared to control rabbits. Interestingly, the number of lymphocytes was significantly increased in not washed feed rabbits compared to wash feed rabbits. The results of the present study suggest that residue of agriculturally used pesticides may affect the body homeostasis of animals. Res. Agric., Livest. Fish.7(3): 439-444,  December 2020

scholarly journals The effect of Ricinus communis seeds extract on reproductive activity and blood values of male rabbits

2019 ◽  
Vol 12 (1) ◽  
pp. 97-106
Author(s):  
AL-Khafaji Nazar
Keyword(s):  
Estrogenic Activity ◽  
Ricinus Communis ◽  
Hematological Parameters ◽  
Abdominal Cavity ◽  
Hemoglobin Concentration ◽  
Reproductive Activity ◽  
Treated Group ◽  
Blood Parameters ◽  
The Body ◽  
Tropical Regions

Ricinus communis L. of Euphorbiaceae family is a widespread plant in tropical regions. It is used in traditional medicines as an anti- fertility agent in India and different parts of the world. The ether soluble portion of the methanol extract of R. communis var minor possesses anti-implantation, anti - conceptive and estrogenic activity in rats and mice when administered subcutaneously.The study was conducted on 10 local breed male rabbits, 1-2 years old, of 1-2 kg body weight. The animals were divided into two groups, control non – treated group and treated group in which animals were treated with single daily dose of 50 mg /kg b. wt. P.O. of decorticated and defatted castor seeds (DDCS) for 14 days. 28th day post treatment, animals were anesthetized by diethyl ether, sacrificed, abdominal cavity was open. The sexual organ (testes, epididymis, prostate and seminal vesical) weighed. In addition to take a biopsy from each one for histopathological changes. The study also included clinical and hematological parameters, in addition to sperm counts and the changes in sperm morphology.Body weight, body temperature increased significantly in treated males. While in non- treated group there were no significant changes. Respiratory rates and heart rate were none significantly changed in treated and non- treated males.Bleeding time none significantly increased in treated males, but increased significantly in none treated males. Clotting times decreased none significantly in treated and non- treated males. The blood parameters including, total erythrocytes count, hemoglobin concentration, PCV% , MCV, MCH, MCHC, total leucocyte and differential leucocyte counts were either increased or decreased none significantly in both groups. The results revealed that the effects of exposure to extract of ricin for 14 days on reproductive efficiency of rabbits, exhibited Significant decrease in weights of testes, epididymis, tails, heads of epididymis, seminal vesicles and prostate in treated males in comparison with those of non- treated males. While the body of epididymis did not show a significant changes.Significant decrease in live sperm numbers, number of sperms in epididymal head, in addition to deformities in high numbers of sperm, including enlarged or small sperms. breaks head, and its detachment, presence of two heads in one sperm, bifurcation of tail and its breaking, sperm coiling in samples from treated males in comparison with those from non-treated males.Histological changes were hyperplasia of lining epithelial cells and vacuolar degenerative changes, loss of spermatogenesis, and spermatocytes necrosis in those from treated males.

scholarly journals Valores hematológicos do gavião carijó Buteo magnirostris (GMELIN, 1788) (AVE, FALCONIFORME)

2017 ◽  
Vol 2 (1) ◽  
pp. 43-54
Author(s):  
Antenor A. Santos ◽  
Adriana A. Joper ◽  
Mizue I. Egami
Keyword(s):  
Blood Cells ◽  
Leukocyte Count ◽  
Hematological Parameters ◽  
Total Count ◽  
Morphological Description ◽  
Differential Leukocyte Count ◽  
Blood Samples ◽  
Green Area ◽  
Blood Smears ◽  
Spherical Core

RESUMO: Os animais utilizados neste trabalho foram fornecidos pelo Departamento de Parque de Área Verde (SP). As amostras de sangue foram colhidas entre agosto de 1999 a novembro de 2001 e determinados os seguintes parâmetros hematológicos: contagem total de eritrócitos (Er), hematócrito (Ht), taxa de hemoglobina (Hb), contagem total e diferencial de leucócitos, índices hematimétricos absolutos VCM e CHCM. Foi realizada também a descrição morfológica das células sanguíneas em extensões sanguíneas após aplicação do método de Leishman. Os valores referentes à série eritrocítica apresentaram as seguintes médias: Er= 2,62 ± 0.48 x 106/µL; Ht=43,16 ± 11,19%; Hb= 14,59 ± 3,38g/dl; VCM=166,33 fL; CHCM= 33,84 ± 2,55g/dl e àqueles referentes a contagem de leucócitos apresentaram média: Lc= 3,95 ± 2,06 x 103/µL; Ht=45,33 ± 9,48%; Eo=17,66 ± 10,40%; B=7,3 ± 3,05%; L= 27,33 ± 11,53% e M= 2 ± 2,30%. Foram observados eritrócitos elípticos, com núcleo heterocromático alongado e citoplasma acidófilo; trombócitos elípticos com núcleo basófilo, cromatina condensada e citoplasma hialino; heterófilos esféricos, com núcleo lobulado e presença de grânulos acidófilos fusiformes; eosinófilos esféricos com núcleo lobulado apresentando grânulos eosinófilos esféricos; basófilos com núcleo volumoso e grânulos basófilos; linfócitos de aspecto irregular, com núcleo esférico e grânulos azurófilos; monócitos esféricos, com núcleo reniforme, cromatina frouxa, nucléolo visível e grânulos azurófilos finos.Palavras-chave: Gavião Carijó; Buteo magnirostris; Hematologia. ABSTRACT: The animals used in this study were provided by the Green Area Park Department (SP). Blood samples were collected from August 1999 to November 2001 and determined the following hematological parameters: total count of erythrocytes (Er), hematocrit (Ht), hemoglobin (Hb), total count and differential leukocyte count, erythrocyte indices absolute MCV and MCHC. It also performed the morphological description of the blood cells in blood smears after application of Leishman method. The figures for the erythrocyte series presented the following averages: Er = 2.62 ± 0:48 x 106 / µL; Ht = 43.16 ± 11.19%; Hb = 14.59 ± 3,38g / dl; VCM = 166.33 fL; MCHC = 33.84 ± 2.55g / dl and those related to leukocyte count showed average: Lc = 3.95 ± 2.06 x 103 / µL; Ht = 45.33 ± 9.48%; And = 17.66 ± 10.40%; B = 7.3 ± 3.05%; L = 27.33 ± 11.53% and M = 2 ± 2.30%. erythrocytes were observed elliptical, with elongated heterochromatic nucleus and cytoplasm acidophilus; thrombocytes Elliptical with basophil nucleus, condensed chromatin and hyaline cytoplasm; Spherical heterophile with lobed nucleus and presence of acidophilic spindle granules; eosinophil-lobed spherical core having eosinophils spherical granules; basophils with large nuclei and basophil granules; irregular aspect of lymphocytes, with spherical core and azurophilic granules; Spherical monocyte, a kidney-shaped core, loose chromatin and nucleoli visible fine azurophilic granules.Keywords: Sparrowhawk Carijó; Buteo magnirostris; Hematology.

scholarly journals Comparison of hematological parameters between single and twin pregnancies in Dorper ewes during gestation, lambing, and postpartum

2022 ◽  
Vol 52 (1) ◽  
Author(s):  
Bianca Paola Santarosa ◽  
Gabriela Nascimento Dantas ◽  
Danilo Otávio Laurenti Ferreira ◽  
Henrique Barbosa Hooper ◽  
Ana Carolina Rusca Correa Porto ◽  
...  
Keyword(s):  
Leukocyte Count ◽  
Hematological Parameters ◽  
Hypochromic Anemia ◽  
Fixed Time ◽  
The Body ◽  
Endogenous Cortisol ◽  
Group 2 ◽  
Twin Pregnancies ◽  
Slight Alteration ◽  
Group 1

ABSTRACT: The hematopoietic system changes during the pregnancy to carry fetal development and maternal needs. This study compared the hematological parameters between ewes with single and twin pregnancies during gestation, delivery, and postpartum. The experiment was conducted on 60 healthy pregnant Dorper ewes that were divided into two experimental groups: Group 1 (G1), with single pregnancies (n=30), and Group 2 (G2), with twin pregnancies (n=30). Blood samples were collected from all ewes at different times: immediately before fixed-time artificial insemination (AI); on day 30, 90, 120, 130, and 140 of pregnancy; immediately after delivery; and at 24h and 48h postpartum. Statistical analysis compared the two groups at different times (P<0.05). Mild, normocytic, and hypochromic anemia was detected in all ewes from AI time and throughout pregnancy from both groups, but did not prove to be of clinical relevance. In the peripartum stage (from the 140th day of pregnancy to 48h postpartum), the ewes with twin pregnancies (G2) exhibited higher erythrogram values and neutrophil:lymphocyte ratio than did ewes with single pregnancies (G1). This indicated a greater hematopoietic adaptation in the body during the development of two fetuses. Except for the eosinophil numbers, all leukogram parameters were influenced by pregnancy in a similar way in both groups, and was characterized mainly by leukocytosis with neutrophilia during peripartum due to the high presence of endogenous cortisol at delivery. Thus, these findings showed that pregnancy was a stressful physiological event that increased the leukocyte count with a slight alteration in the erythrogram of Dorper ewes.

scholarly journals EFFECT OF USING DIFFERENT LEVELS PERCENTAGES OF SAND TREATED SMUT WHEAT ON HYSIOLOGICAL PARAMETER OF BROILER

2008 ◽  
Vol 32 (2) ◽  
pp. 1-16
Author(s):  
Maha A. Al-Hemairi
Keyword(s):  
Leukocyte Count ◽  
Fungal Growth ◽  
Blood Parameters ◽  
The Other ◽  
Poultry Farm ◽  
Physical Treatment ◽  
Differential Leukocyte Count ◽  
Enzymes Activity ◽  
Different Levels

This study was carried out at the poultry farm of the college ofveterinary medicine / Baghdad University during 12/5/2004 – 6/7/2004 tostudy the effect of substitution of smut wheat instead of intact wheat inpoultry ration at the following levels (0%, 25% , 50% and 100%)representing the following groups (T0, T1, T2 and T3), respectively. Physicaltreatment was obtained for smut wheat by polishing the seed by sand toremove the superficial fungal growth. Each group was sub divided into twosub groups, one was physically treated with sand and the other was nottreated to study the hematology traits at 28 and 56 days of the experiment.Characterristics included in this study were PCV, Hb, RBC, WBC,differential leukocyte count and some of serum blood enzymes activity(ALT, AST and AP). The results obtained revealed the following:Replacing partial of smut wheat at the level of 50% or total 100%caused an imbalance of blood parameters by decreasing PCV, Hb, RBC,WBC and increased blood enzymes activity of ALT, AST and decreased APactivity. Results of differential leukocyte count revealed that heterophilswere increased while lymphocytes were decreased significantly (P<0.05) at28 and 56 days of age. Physical treatment of smut wheat by sand improve theblood parameters by rebalancing these parameter in comparison with these ofuntreated group .

scholarly journals Biodegradable Zinc Oxide Nanoparticles Doped with Iron as Carriers of Exogenous Iron in the Living Organism

2021 ◽  
Vol 14 (9) ◽  
pp. 859
Author(s):  
Paula Kiełbik ◽  
Aneta Jończy ◽  
Jarosław Kaszewski ◽  
Mikołaj Gralak ◽  
Julita Rosowska ◽  
...  
Keyword(s):  
Hematological Parameters ◽  
Iron Concentration ◽  
Living Organism ◽  
Blood Parameters ◽  
The Body ◽  
Zno Nanoparticle ◽  
Hepcidin Expression ◽  
Adult Mice ◽  
Tissue Iron ◽  
Average Size

Iron plays an important role in various crucial processes in the body and its deficiency is considered currently as a serious health problem. Thus, iron supplementation strategies for both humans and animals need to be effective and safe. According to our previous studies, zinc-based nanoparticles provide safe, biodegradable, fast and efficient transport system of orally given substances to the tissues. In the current manuscript we present results of a study aimed at investigation of the ZnO nanoparticle-based Fe supplementation system (average size 100 × 250 nm). Nanostructures were orally (gavage) administered to adult mice. Animals were sacrificed at different time points with collection of blood and internal organs for analyses (tissue iron concentration, hepatic level of hepcidin, blood parameters, liver and spleen levels of ferritin, histopathology). Initial experiment was performed to compare the biological effect of doping type (Fe3+ doping vs. a mixture of Fe3+ and Fe2+). Then, the effect of acute/chronic exposure models was determined. The increase in ferritin, along with improved, crucial hematological parameters and lack of the influence on hepcidin expression indicated the chronic application of Fe3+,2+ doped ZnO nanostructures to be the most effective among tested.

Hematological Indicators of Moose in Domestication Process

2020 ◽  
Keyword(s):  
Hematological Parameters ◽  
Physiological State ◽  
Blood Parameters ◽  
The Body ◽  
Sex Characteristics ◽  
Hematological Indices ◽  
Blood Indices ◽  
Hematological Indicators ◽  
Age And Sex ◽  
Selection Of

Presented hematological indices for a stress-free selection of material from moose in the process of domestication depending on age and sex characteristics. Fluctuations in the blood parameters of moose have been established within various limits, taking into account the influence of various factors - the climatic, environmental, technological, physiological state of the animal, and age and sex characteristics. Considerable attention is paid to the selection of blood from moose, one of the most labor-intensive processes when working with them. Some tendency to fluctuations in hematological parameters in young moose has been recorded. This, possibly, is associated with a decrease in the action of colostral immunity, the formation of a specific defense of the body. Differences in blood indices in moose depending on gender were unreliable. However, in males, some of them - in terms of the content of erythrocytes, basophils, and monocytes - was higher than in females, and vice versa, in females - the number of leukocytes, lymphocytes, and eosinophils. It was shown that in young animals of 21-50 days of age, a neutrophilic profile prevails with increased content of segmented forms, and in adults - a lymphocytic profile. Compared with moose, which are in the process of domestication in animals living in natural conditions, expressed leukocytosis was recorded. As a result of the studies, the estimated norms of morphological parameters of the blood of moose in the process of domestication are established, depending on age and gender.

scholarly journals Evaluation of Effect of Melatonin on Hematological Parameters in Patients of Periodontitis

2019 ◽  
pp. 46-49
Author(s):  
Anagha Marawar ◽  
Pramod Marawar ◽  
Nandal D H ◽  
Rahul Kunkulol ◽  
Sandeep Narwane
Keyword(s):  
Leukocyte Count ◽  
Hematological Parameters ◽  
Total Leukocyte Count ◽  
Differential Leukocyte Count ◽  
Erythrocyte Sedimentation ◽  
Significant Difference ◽  
Group A ◽  
Group B ◽  
Root Planning ◽  
Scaling And Root Planning

Introduction: Considering the immunity enhancing property of melatonin, a study on the evaluation of the effect of melatonin on the hematological parameters in patients suffering from Periodontitis.  Methods: This study was conducted in the Department of Periodontics, Rural Dental College, Loni. Patients of chronic Periodontitis, of age between 18 to 65 years of either gender ready to give informed consent to participate in the study were included. Postoperative patients, patients having night duties, drivers and those using heavy machinery, pregnant women, lactating mothers, patients with any clinically significant systemic disease and patients on any other drugs were excluded from the study. Patients were divided into two Groups. Group B received scaling and root planning (SRP) and melatonin 3 mg per day were compared to Group A which received SRP only. The Total Leukocyte count, Differential Leukocyte count and Erythrocyte sedimentation rate of each patient was studied at a baseline, 1st (30 days) visit, 2nd (60 days) visit and 3rd (90 days) visit. Results: Both the groups consisted of 80 subjects each. Group A consisted of 71.25% male and 28.75% female patients. There were 56.25% male and 43.75% female patients in Group B. With respect to visit 3, the TLC was statistically lower in Group B. The neutrophil count of Group B was significantly lower during visit 3 as compared to Group A. The lymphocyte count of Group B was significantly lower than that of Group A during the visit 1, and also significantly higher during visit 3 on comparison with Group A. There was no statistically significant difference in the groups with respect to eosinophil and basophil count. The Monocyte count of Group B was statistically lower when compared to that of Group A. There was no statistically significant difference between ESR of Group A & Group B.  Conclusions: It can be concluded that melatonin has a positive effect on TLC and differential count of patients of periodontitis, conferring a new facet to the management of periodontitis and an attempt to impede the disease progression. Keywords: Melatonin; Hematological parameters; Periodontitis; Total Leukocyte count; Differential Leukocyte count; Erythrocyte sedimentation rate; Scaling and Root Planning.

scholarly journals Comparison of hematological parameters in mild and severe COVID-19 infected patients: A retrospective observational study

Biomedicine ◽  
2021 ◽  
Vol 41 (4) ◽  
pp. 799-804
Author(s):  
Tania Eltrida Pinto ◽  
Rithesh Joseph D'Cunha ◽  
Shannon Fernandes ◽  
. Nireeksha ◽  
Gurumurthy T.
Keyword(s):  
Medical Records ◽  
Leukocyte Count ◽  
Hematological Parameters ◽  
Demographic Data ◽  
Severe Disease ◽  
Disease Group ◽  
Differential Leukocyte Count ◽  
Mild Disease ◽  
Significant Difference ◽  
D Dimer

Introduction and Aim: With the coronavirus disease 2019 (COVID-19) pandemic raging on, there is a need to identify clinical and laboratory predictors which predict progression towards severe and fatal forms of this illness. Our study aims to evaluate the ability of hematologic and biochemical biomarkers to discriminate between patients with and without severe or fatal forms of COVID-19.   Materials and Methods: A retrospective study was conducted on 200 Covid positive patients;100 with mild disease and 100 with severe disease. Medical records were reviewed to collect demographic data and results of the following blood investigations were noted at admission: Hb, Platelet count, Total and Differential leukocyte count, CRP, AST, ALT, LDH, Ferritin and D-Dimer. Comparative analysis was performed between the 2 groups.   Results: A significant difference in the basophil count (mean 2.35 and 5.92) among those with mild and severe disease respectively was noted as also with the eosinophil count (mean 6.88 and 1.79). The levels of CRP were higher in those with severe disease as compared to the mild disease group (mean 276.29 and 65.23). Ferritin levels were markedly increased severe disease patients (mean 1275.66 and 533.94). D-dimer was markedly increased in COVID-19 patients with severe disease (mean 3813.91 ng/ml) compared to those with mild disease group (mean 521.78 ng/ml).   Conclusion: Hematological and biochemical markers positively correlate to the severity of covid infection, thus highlighting their role in the early diagnosis of the disease and can act as independent markers in predicting severity and prognosis of disease.

scholarly journals IMMUNOMODULATING EFFECTS OF THE PURIFIED HEV B 13 FRACTION ON SEPTIC RATS

2017 ◽  
Vol 30 (2) ◽  
pp. 93-97 ◽  
Author(s):  
Maxley Martins ALVES ◽  
Lilhian Alves de ARAÚJO ◽  
Fátima MRUÉ ◽  
Clayson Moura GOMES ◽  
Milton Adriano Pelli de OLIVEIRA ◽  
...  
Keyword(s):  
Inflammatory Responses ◽  
Leukocyte Count ◽  
Multiple Organ ◽  
The Body ◽  
Organ Injury ◽  
Total Leukocyte Count ◽  
Histopathological Analysis ◽  
Differential Leukocyte Count ◽  
Sepsis Induction ◽  
Life Threatening

ABSTRACT Background: Sepsis is a potentially life-threatening complication of an infection that occurs when chemicals released into the bloodstream to fight the infection trigger inflammatory responses throughout the body, especially in the acute phase of the disease, producing excessive pro-inflammatory cytokines, leading to multiple organ injury and death. The Hev b 13 fraction has demonstrated biological activity capable of inducing IL-10 production and shrinking inflammatory disease lesions. Aim: To investigate the immunomodulating effects of the Hev b 13 fraction on septic rats. Methods: Acinetobacter baumannii was injected into the peritoneal cavity of the animals after sustaining a lesion in the pancreas, with the stomach as an entry point. After 10 h of infection, they were euthanized for blood and lung collection, followed by total and differential leukocyte count, determination of cytokine level and histopathological analysis. Results: Administering a single dose of the Hev b 13 fraction 2 h after sepsis induction significantly decreased total leukocyte count. Higher IL-10 and IL-4 and lower IL-6 production shrank the lung tissue lesions compared to the control groups. Conclusion: The Hev b 13 fraction exhibits an anti-inflammatory tendency, with potential for sepsis treatment.

scholarly journals Growth and blood parameters of soybean based milk replacer fed and naturally suckled Black Bengal kids

2021 ◽  
Vol 50 (1) ◽  
pp. 22-27
Author(s):  
US Alam ◽  
A Khatun ◽  
RC Chanda ◽  
MH Alam ◽  
MN Islam ◽  
...  
Keyword(s):  
Blood Cell ◽  
Leukocyte Count ◽  
Live Weight ◽  
Blood Parameters ◽  
Milk Replacer ◽  
Body Measurement ◽  
Differential Leukocyte Count ◽  
Treatment Groups ◽  
Significant Difference ◽  
Rbc Count

The present study was aimed to determine growth and blood parameters of soybean based milk replacer fed kids and naturally suckled counterparts in three villages of Mymensingh Sadar, Bangladesh. Twelve Black Bengal kids were allocated into two treatment groups; one group (n=6) was reared with milk replacer while the other (as control; n=6) was reared naturally keeping with their dam. Live weight and body measurement of kids were recorded weekly. Blood samples were collected from 8 weeks old kids to determine their red blood cell (RBC), white blood cell (WBC), haemoglobin (Hb), packed cell volume (PCV) and differential leukocyte count (DLC). The average initial live weight of the suckled and replacer fed kids did not show significant difference (p<0.05). The average final live weight was significantly (p<0.05) higher in artificially fed males (7.7±0.18 kg) than naturally suckled male (6.89±0.24 kg) kids. Body length, wither height and heart girth were significantly (p<0.05) higher in milk replacer fed male kids than naturally suckled counterparts. RBC count was significantly (p<0.05) higher in artificially fed group than naturally suckled kids. Hb, PCV and DLC did not differ significantly (p>0.05) between treatment groups. In conclusion, feeding with soybean based milk replacer enhanced growth of kids comparing naturally reared counterparts. Bang. J. Anim. Sci. 2021. 50 (1):22-27

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