scholarly journals Creation of pea recombinant lines with increased level of adaptability to drought conditions

Author(s):  
V. I. Sichkar ◽  
A. O. Vasylenko ◽  
A. I. Kryvenko ◽  
R. V. Solomonov

Introduction. Peas are among the most common legumes in Ukraine, so the creation and introduction into production of new varieties has a significant economic effect. The main purpose of its breeding is to create highly adaptable to biotic and abiotic factors varieties, the yield of which would be stable for many years. Matcrials and mcth0ds. Field studies were conducted in the central zone of Odessa region during 2018–2021. Control nursery, ecological, preliminary and competitive testing were sown in plots 10 m long with a seeder “Klen-1.5C”, sowing of breeding and collection nurseries was carried out manually in a wide rows with 45 sm. In the process of vegetation conducted phenological ob- servations, the necessary accounting and evaluation. Plots of control, ecological, preliminary and competitive testing were harvested with a breeding combine “Sampo-130”, plants of selection and collection nurseries were tied into sheaves and then threshed on a thresher. Seed yield was calculated at 14% humidity. Rcsults and discussi0n. Under arid condi- tions in 2018–2020, the yield of all varieties and breeding lines was low, which indicates the need to strengthen breeding research to improve the adaptive characteristics of the new source material. Analysis of the results of our own research and a large volume of literature sources show that the creation of varieties with a more stable level of productivity will take place gradually through the consistent accumulation of positive gene loci using complex stepwise crosses and large volumes of hybrid populations of early generations, and effective selection highly adaptive forms. The network of ecological tests is of great importance. Under optimal moisture conditions, the yield of the best varieties reaches 5.0 t / ha, and in some recombinant lines exceeds this value. The identification of donors and sources of drought and heat resistance among the world gene pool is becoming increasingly important. It is especially important to identify the genetic loci of these traits by genetic molecular research methods.

Author(s):  
I. A. Meremyanina I. A. ◽  
◽  
V.V. Kenyz V.V.

The article is based on the creation and evaluation of new varieties and complex hybrid populations of alfalfa with economically valuable traits and properties.Varieties with high productivity of green mass and seeds have been created and studied.


Author(s):  
V.N. Zolotarev ◽  
◽  
I.S. Ivanov ◽  
O.N. Lyubtseva

Based on the analysis of data available in the literature and our own experimental material on phytocenotic selection of the stony stalk (Bromopsis inermis Holub.) the important role of competition between plants in the field for the creation of new varieties of perennial grasses that provide high yields of feed polyvid agrophytocenoses is shown.


Author(s):  
O. V. Levakova ◽  
L. M. Eroshenko

The article presents the results of the analysis of the biochemical composition of selected in the competitive variety testing of new varieties and promising lines of spring barley, studied on the experimental field ISA-branch of FGBNU FNAC VIM in 2016-2018. Result of research in different years of water availability among the rooms was allocated a group of high-protein barley with improved feed value: 4 varieties, 2 of which are on the state variety testing, and 2 promising lines. The range of variation of protein content in the grain of the selected samples varied from 13.1 to 14.5 %, lysine – from 0.48 to 0.52 %, weight of 1000 grains-from 48.0 to 53.5 g. According to the data obtained the highest value of protein in grain was observed in krupnosortnogo varieties Zlatoyar (GSI), amounting on average for 14.5 %. The increase was equal to 1.1% in relation to the control variety Yaromir. With an average yield of 6.52 t/ha in the years of research grade Accounting have provided protein per hectare in the amount of 0,94 t/ha. A new variety Sudar´ (GSI) and breeding line 149/4-12 h 788 and 129/1-14 h 907 this indicator has been less productive, however, exceeded the grade Yaromir 0.10 to 0.16 t/ha. Given the higher productivity of new promising varieties and breeding lines, the collection of lysine per unit area they have 12.7, 30.8 per cent was higher than that of the Promej class and 10.6-23.6% more than in the variety Yaromir, which indicates their high biological value. Selected hulless line 1137 h is also an encouraging source of protein and other nutrients of plant origin. The selected lines and new varieties, regardless of weather conditions, were able to ensure the production of high-protein and sources' grain for feed purposes, while maintaining productivity.


1992 ◽  
Vol 6 (1) ◽  
pp. 108-112 ◽  
Author(s):  
W. James Grichar

Field studies were conducted from 1986 through 1988 to evaluate various herbicides for yellow nutsedge control and peanut yields. Three applications of pyridate provided control comparable to two applications of bentazon with yellow nutsedge regrowth beginning 3 to 4 wk after application depending on moisture conditions. Crop oil concentrate did not improve the activity of pyridate. Flurtamone provided control comparable with that of metolachlor. Nutsedge control with fomesafen was erratic with peanut injury noted. Peanut yields did not reflect the competitive nature of nutsedge.


2021 ◽  
pp. 34-37
Author(s):  
Р.Х. Беков

Рассмотрены важные аспекты использования геноносителей различных маркерных признаков при создании и селекции гетерозисных гибридов томата для защищенного грунта. Наряду с общими требованиями к родительским компонентам, касающимися продуктивности растений, скороспелости, устойчивости к болезням и т. д., сегодня при селекции на гетерозис для защищенного грунта к исходному материалу предъявляют более высокие требования по наличию хозяйственно полезных признаков. Эти требования касаются типа роста растений, формы, окраски и качества плодов, типа кисти, формы плодоножки и т. д. Представлены характеристики исходного материала для создания гетерозисных гибридов томата, а также даны характеристики полученных гибридов. Исследования, проведенные в отделе селекции ВНИИО (ВНИИ овощеводства – филиал ФГБНУ ФНЦО), показали большую перспективность использования при гетерозисной селекции томата для защищенного грунта геноносителей различных маркерных признаков, особенно признаков, определяющих коричневую окраску эндосперма семян (гены bs и bs-2) и несочлененную плодоножку (ген j-2). Созданные с использованием этих маркерных признаков гибриды и селекционные линии по-своему уникальны и являются приоритетным достижением этого научного учреждения, так как они представляют большой интерес для гетерозисной селекции томата в качестве исходного материала. Целый ряд гибридов F1 включен в Госреестр селекционных достижений, допущенных к использованию (Удача, Голубчик, Клад овощевода, Заур и др.). В связи с развитием фермерского и любительского овощеводства в последние годы определенное внимание было уделено созданию новых мутантных линий с розовыми, малиновыми и темно-коричневыми плодами с высокими вкусовыми качествами (учитывая, что образцы такого типа пользуются повышенным спросом у населения и стоят дороже). При создании новых гетерозисных гибридов были учтены эти признаки родительских форм. Important aspects of the use of gene carriers of various marker traits in the creation and selection of heterotic tomato hybrids for greenhouse industry are considered. Along with the general requirements for the parent components concerning plant productivity, precocity, resistance to diseases, etc., today, when breeding for heterosis for greenhouse industry, higher requirements are imposed on the source material for the presence of economically useful features. These requirements relate to the type of plant growth, shape, colour and quality of fruits, brush type, stem shape, etc. The characteristics of the initial material for creating heterotic tomato hybrids are presented, as well as the characteristics of the resulting hybrids are given. Studies conducted in the breeding department of the ARRIVG (ARRIVG – a branch of the FSBI FSCV) showed great prospects for the use of gene carriers of various marker traits, especially those that determine the brown colour of the seed endosperm (bs and bs-2 genes) and an undifferentiated peduncle (j-2 gene) in the heterosis breeding of tomatoes for greenhouses. Hybrids and breeding lines created using these marker traits are unique in their own way and are a priority achievement of this scientific institution, since they are of great interest for the heterosis breeding of tomato as a basic material. A number of F1 hybrids are included in the State Register of Breeding Achievements allowed for use (Udacha, Golubchik, Klad ovoschevoda, Zaur, etc.). Due to the development of farming and amateur vegetable growing in recent years, some attention has been paid to the creation of new mutant lines with pink, crimson and dark brown fruits with high taste qualities (given that samples of this type are in high demand among the population and are more expensive). When creating new heterotic hybrids, these features of the parent forms were taken into account.


2018 ◽  
Vol 69 (12) ◽  
pp. 1237 ◽  
Author(s):  
G. C. Wright ◽  
M. G. Borgognone ◽  
D. J. O Connor ◽  
R. C. N. Rachaputi ◽  
R. J. Henry ◽  
...  

Breeding for improved blanchability—the propensity of the testa (skin) to be removed from the kernel following rapid heat treatment—is a priority for improvement in the Australian Peanut Breeding Program (APBP). Easy removal of the testa by blanching is required for processing of peanuts into peanut butter and various other confectionary products. Thus, blanchability is an economically important trait in any newly released cultivar in Australia. A better understanding of the range of genetic variation, nature of inheritance and genotype×environment (G×E) interactions, and the development of a low-cost method to phenotype in early generations, could speed up breeding for this trait. Studies were conducted to develop a low-cost, rapid method utilising minimal amounts of seed to phenotype in early generations, along with an assessment of G×E interactions over a range of years and environments to derive optimal selection protocols. Use of a smaller kernel sample size than standard (50 vs 200g) was effective for accurately assessing blanchability in breeding lines and could allow selection in early generations (e.g. in seed produced from a single F2 plant where seed supply is adequate). G×E interaction for blanchability was shown to be very low. Genotypic variance explained 62–100% of the total variance for blanchability, assessed in two diverse germplasm pools including 107 accessions in the USA mini-core over three environments and multiple APBP breeding lines grown over nine different years–environments. Genotypic correlations between all environments were very high (~0.60–0.96), with heritability for the blanchability trait estimated to be very high (0.74–0.97) across the 13 trials. The results clearly demonstrate that effective selection for improved blanchability can be conducted in early generations and in a limited number of contrasting environments to ensure consistency of results.


2021 ◽  
pp. 15-23
Author(s):  
V. F. Pivovarov ◽  
A. V. Soldatenko ◽  
O. N. Pyshnaya ◽  
L. K. Gurkina

The article reflects the issues of the current state of scientific research at the FSBSI FSVC, shows the directions and prospects for the development of selection and seed production of vegetable crops. The main mission of the selection and seed-growing work of the FSVC is the creation and reproduction of varieties and hybrids of vegetable and melon crops of a new generation, characterized by resistance to abiotic and biotic stressors, high productivity and product quality. One of the main ways to increase the efficiency of the breeding process is the use of modern methods of biotechnology and molecular genetics, which make it possible to obtain new genotypes and reduce the time of breeding. Success has been achieved in the creation of homozygous lines of cabbage, squash, cucumber, carrots. For the first time, it was possible to complete a full cycle of obtaining doubled radish haploids in microspore culture in vitro. The institution pays special attention to immunological research on the resistance of crops and new varieties to diseases, as well as protecting plants from pests and diseases. Phytosanitary monitoring, assessment and selection of resistant genotypes are carried every years. One of the important theoretical and practical problems solved at the institution is the development of environmentally friendly products based on preparations obtained from plants with a high content of biologically active compounds with adaptogenic properties. In the center, a new direction of research in traditional breeding has received significant development - the creation of varieties of vegetable plants with an increased content of biologically active substances and antioxidants, on the basis of which functional products are created. The persistence of modern varieties and hybrids of table carrots, depending on the biochemical composition, has been studied. Methods for enriching vegetable plants with micronutrients are being developed. A large amount of scientific research is being carried out on the development of technologies for the cultivation of new varieties and hybrids, as well as technologies for their seed production. At the branches located in different soil and climatic conditions, hospitals were established to study the interaction of factors controlling soil fertility and the productivity of vegetable crops in agrocenoses (crop rotation, soil treatment systems, fertilizers and plant protection). In general, at the FSBSI FSVC, all research is aimed at developing and improving methods in breeding and seed production of vegetable crops, creating new breeding achievements and developing varietal technologies for their cultivation.


2021 ◽  
pp. 65-67
Author(s):  
O. P. Kigashpaeva ◽  
A. V. Gulin ◽  
L. P. Lavrova

The varieties and hybrids of cucumbers cultivated in the Astrakhan region are insufficiently productive and are largely affected by a number of diseases. The introduction of innovative developments into production, in particular, new varieties and hybrids of domestic selection, largely solves the problem of import substitution and food security. The goal is to use promising breeding lines and their offspring as genetic sources and donors when creating new varieties and hybrids for open field conditions in southern Russia.Methods. Experiments were carried out in the fields of Precaspian agrarian federal scientific center of the Russian academy of sciences, according to the methods used in the Astrakhan region. Within three years, 47 collection samples were tested.Results. The article presents the results of studying the 12 most promising ones, distinguished by a complex of valuable economic traits, in particular by yield, amicability of fruit setting, which are of interest for further breeding work. According to the results of the data obtained, we can say that the best were the lines: 13-88RZ, 13-101RZ, Gherkins, KRASTAVAC, Courage, Zina, they can be used to create new varieties and hybrids of cucumber as donors of a predominantly female type of flowering, harmonious yield.


2019 ◽  
Vol 95 (8) ◽  
Author(s):  
Wietse de Boer ◽  
Xiaogang Li ◽  
Annelein Meisner ◽  
Paolina Garbeva

ABSTRACT There is increasing evidence that microbial volatile organic compounds (mVOCs) play an important role in interactions between microbes in soils. In this minireview, we zoom in on the possible role of mVOCs in the suppression of plant-pathogenic soil fungi. In particular, we have screened the literature to see what the actual evidence is that mVOCs in soil atmospheres can contribute to pathogen suppression. Furthermore, we discuss biotic and abiotic factors that influence the production of suppressive mVOCs in soils. Since microbes producing mVOCs in soils are part of microbial communities, community ecological aspects such as diversity and assembly play an important role in the composition of produced mVOC blends. These aspects have not received much attention so far. In addition, the fluctuating abiotic conditions in soils, such as changing moisture contents, influence mVOC production and activity. The biotic and abiotic complexity of the soil environment hampers the extrapolation of the production and suppressing activity of mVOCs by microbial isolates on artificial growth media. Yet, several pathogen suppressive mVOCs produced by pure cultures do also occur in soil atmospheres. Therefore, an integration of lab and field studies on the production of mVOCs is needed to understand and predict the composition and dynamics of mVOCs in soil atmospheres. This knowledge, together with the knowledge of the chemistry and physical behaviour of mVOCs in soils, forms the basis for the development of sustainable management strategies to enhance the natural control of soil-borne pathogens with mVOCs. Possibilities for the mVOC-based control of soil-borne pathogens are discussed.


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