scholarly journals Effect of Clove essential oil (Syzygium aromaticum) against the growth of dandruff scalps-causing fungal pathogen using Kirby- Bauer method in vitro

2021 ◽  
Vol 3 (1) ◽  
pp. 41-48
Author(s):  
Khusnul Khusnul ◽  
Puput Putuh Anisa ◽  
Dewi Peti Virgianti
Keyword(s):  
Essential Oil ◽  
Fungal Pathogen ◽  
Fungal Infections ◽  
Pathogenic Fungi ◽  
Negative Control ◽  
Diffusion Method ◽  
Microsporum Gypseum ◽  
Syzygium Aromaticum ◽  
Contributing Factors

One of the strongest contributing factors in dandruff scalps is the presence of fungal pathogen or called as Malassezia. Several studies have found a connection between Malassezia and scalp health, one of which is caused by pathogenic fungal infections including Pityrosporum ovale, Microsporum gypseum, and Candida albicans. Clove flowers are known to contain eugenol and its derivative compounds which have antimicrobial, antifungal, antiseptic, and local anesthetic activity. This research aims to determine the effect of clove flower essential oil (Syzygium aromaticum) against some dandruff-causing fungi. The research method was to test the inhibitory power of clove flower essential oil using the diffusion method (Kirby Bauer technique). Samples of pathogenic fungi were cultured on SDA media (Saboroud Dextrose Agar) and given discs that had been given several concentrations of clove flower essential oil. The concentrations used were 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%, positive control using 2% ketoconazole and negative control using virgin coconut oil (VCO). The results showed a significant effect between the ability of essential oils to inhibit the growth of some fungi that cause dandruff. The treatment of clove flower essential oil against M. gypseum at the concentration of 10% has an inhibitory ability of 33,05 mm and at the concentration of 100% amounted to 63.95 mm. Meanwhile, the inhibition percentage in P. ovale with a concentration of 10% and 100% of extract were 14,75 mm and 46,15 mm, respectively. The inhibition rate in C. albicans with the concentration of 10% extract and 100% of extract was 13,5 mm and 39,35 mm. The research shows that clove flower essential oil inhibits the growth of several pathogenic fungal in vitro.

scholarly journals Antifungal Activity of the Essential Oil of Echinops kebericho Mesfin: An In Vitro Study

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Tamirat Bekele Beressa ◽  
Serawit Deyno ◽  
Paul E. Alele
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Cryptococcus Neoformans ◽  
Antioxidant Properties ◽  
Pathogenic Fungi ◽  
Diffusion Method ◽  
Potential Candidate ◽  
Standard Drug

Background. Echinops kebericho is an endemic medicinal plant in Ethiopia widely used in the treatment of infectious and noninfectious diseases. Essential oils are known for their antibacterial, antifungal, antiviral, insecticidal, and antioxidant properties. This study evaluated the antifungal activity of essential oil from E. kebericho against four common pathogenic fungi and two standard strains. Methods. The essential oil was obtained by hydrodistillation. The antifungal screening was done by agar well diffusion method. Minimal inhibitory concentrations (MICs) were determined by broth microdilution. Minimal fungicidal concentrations (MFCs) were determined by subculturing fungal strains with no visible growth onto a Sabouraud dextrose agar (SDA) plate. Results. Candida albicans and Cryptococcus neoformans were highly sensitive while Aspergillus flavus did not show sensitivity up to 1 mg/ml of essential oil; MICs ranged from 0.083 mg/ml to 0.208 mg/ml. Concentration and fungal species showed significant dose-dependent associations ( p < 0.0001 ) with antifungal activity. The MICs of essential oil were comparable to those of the standard drug (fluconazole) against C. glabrata and C. krusei. The lowest MFC of the essential oil was observed against Candida parapsilosis (0.145 mg/ml) while the highest MFC was against Candida krusei (0.667 mg/ml). Conclusion. Echinops kebericho essential oil showed noteworthy antifungal activity against Cryptococcus neoformans, Candida albicans, and Candida glabrata and could be a potential candidate for further antifungal drug development.

scholarly journals Ethnobotanical Survey of Dracaena cinnabari and Investigation of the Pharmacognostical Properties, Antifungal and Antioxidant Activity of Its Resin

Plants ◽  
2018 ◽  
Vol 7 (4) ◽  
pp. 91 ◽  
Author(s):  
Mohamed Al-Fatimi
Keyword(s):  
Skin Diseases ◽  
Antioxidant Activities ◽  
Fungal Infections ◽  
Biological Activities ◽  
Gastrointestinal Diseases ◽  
Diffusion Method ◽  
Microsporum Gypseum ◽  
Ethnobotanical Survey ◽  
Dracaena Cinnabari

Dracaena cinnabari Balf. f. (Dracaenaceae) is an important plant endemic to Soqotra Island, Yemen. Dragon’s blood (Dam Alakhwin) is the resin that exudes from the plant stem. The ethnobotanical survey was carried out by semi-structured questionnaires and open interviews to document the ethnobotanical data of the plant. According to the collected ethnobotanical data, the resin of D. cinnabari is widely used in the traditional folk medicine in Soqotra for treatment of dermal, dental, eye and gastrointestinal diseases in humans. The resin samples found on the local Yemeni markets were partly or totally substituted by different adulterants. Organoleptic properties, solubility and extractive value were demonstrated as preliminary methods to identify the authentic pure Soqotri resin as well as the adulterants. In addition, the resin extracts and its solution in methanol were investigated for their in vitro antifungal activities against six human pathogenic fungal strains by the agar diffusion method, for antioxidant activities using the DPPH assay and for cytotoxic activity using the neutral red uptake assay. The crude authentic resin dissolves completely in methanol. In comparison with different resin extracts, the methanolic solution of the whole resin showed the strongest biological activities. It showed strong antifungal activity, especially against Microsporum gypseum and Trichophyton mentagrophytes besides antioxidant activities and toxicity against FL-cells. These findings confirm and explain the traditional uses of the resin for the treatment of skin diseases and mouth fungal infections.

scholarly journals Determination of chemical composition and evaluation of Antioxidant, and Antimicrobial activities of Clove Oil obtained from Syzygium Aromaticum Moroccan species

2020 ◽  
Vol 11 (2) ◽  
pp. 2568-2574
Author(s):  
Siham. Yanisse ◽  
Hajar. Benhhadou ◽  
Miloud EL Karbane ◽  
Brahim. Mojemmi ◽  
Housaine. Tligui ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Fungal Infections ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Effect ◽  
Diffusion Method ◽  
Syzygium Aromaticum ◽  
Clove Oil

The composition, antimicrobial and antioxidante activities of essential oil obtained from Syzygium aromaticum (Moroccan species), were studied. The extraction was carried out by hydro-distillation and analyzed by gas chromatography-mass spectrometryy (GC-MS). The antioxidant effect was evaluated using two in vitro antioxidant assay using DPPH and FRAP methods, 2,2-Diphenyil picrylhydrazyl free radical (DPPH) and Total reducing ability determination by Fe3+–Fe2+ transformation Method (FRAP). And the antimicrobial effects of essential oils were evaluated by the disk diffusion method.  The determination of composition showed that Twenty-four compounds were identified in the extracts of clove oil by gas chromatographyy/mass spectrometry. The constituents major of clove buds was Eugenol with percentage (63, 68 %).  The result shown that clove oil had an important effect antioxidant in two in vitro assays including reducing power FRAP and DPPH radical scavenging. The result has been compared to that of some natural antioxidant. Based on the measurement of the diameter of inhibition, a moderate to high antimicrobial activity, according to oils was revealed against all yeast strains and moderate against bactéria strains. The Eo has showed a considerable antioxidant effect and antimicrobial activity against bacterial and fungus strains, deserving further investigation for clinical application in thetreatment of fungal infections.

scholarly journals In Vitro Activity of Lawsonia inermis (Henna) on Some Pathogenic Fungi

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Elham Abdelbasit Suleiman ◽  
Elbasheir Ahmed Mohamed
Keyword(s):  
Antifungal Activity ◽  
Fungal Infections ◽  
Pathogenic Fungi ◽  
Diffusion Method ◽  
In Vitro Activity ◽  
Lawsonia Inermis ◽  
Traditional Use ◽  
Yeast Strains ◽  
Inhibition Concentration

The present study was conducted to investigate antifungal activity of Lawsonia inermis (Henna plant). Leaf samples of the plant were collected from Eastern Nile of Khartoum State, Sudan. Ethanol and petroleum ether extracts were obtained by maceration (cold method). The extracts were bioassayed in vitro to know their bioactivity to inhibit the growth of tested fungi. The cup-plate agar diffusion method was adopted to assess the antifungal activity of the extracts against tested yeasts, while agar incorporated method was used for other molds. Both extracts revealed antifungal activity against all yeast strains except Pichia fabianii which was found resistant to both ethanol and ether extracts. The results displayed antifungal activity against tested fungi. Minimum inhibition concentration (MIC) of 5, 7.5, and 10 mg/mL was found to inhibit the growth of tested dermatophytes. The obtained results revealed antifungal activity of Henna leaves extracts which support the traditional use of Henna in therapy of fungal infections. The possibility of therapeutic use of Sudanese Henna as antifungal agent is recommended.

scholarly journals In Vitro Antifungal and Topical Anti-Inflammatory Properties of Essential Oil from Wild-Growing Thymus vulgaris (Lamiaceae) Used for Medicinal Purposes in Algeria: A New Source of Carvacrol

2020 ◽  
Vol 88 (3) ◽  
pp. 33 ◽  
Author(s):  
Mohamed Nadjib Boukhatem ◽  
Noureldien H. E. Darwish ◽  
Thangirala Sudha ◽  
Siham Bahlouli ◽  
Dahbia Kellou ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Fungal Infections ◽  
Positive Control ◽  
Diffusion Method ◽  
Thymus Vulgaris ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Anti Inflammatory

The aim of this study is to investigate the Thymus vulgaris essential oil (TVEO) as an antifungal agent in aromatherapy and/or as an active ingredient in the prevention or management of topical inflammatory diseases. The chemical composition of TVEO was determined with gas chromatography and revealed the presence of 25 compounds. Carvacrol was found to be the major component (56.8%). Antifungal action of TVEO was determined in vitro by using different methods. By the disc diffusion method, TVEO showed more potent antifungal activity against Candida strains than the positive control. The diameter of inhibition zone (DIZ) varied from 34 to 60 mm for Candida yeasts. Significantly higher antifungal activity was observed in the vapor phase at lower quantities. Candida albicans and C. parapsilosis were the most susceptible strains to the oil vapor with DIZ varying from 35 to 90 mm. The minimum inhibitory concentrations (MIC) of yeast were determined with an agar dilution method and revealed that MIC varied from 0.3 to 0.15 µL/mL for yeast species. The topical anti-inflammatory potential of TVEO was also explored in vivo with the croton oil-induced ear edema assay. TVEO exhibited a potent anti-inflammatory effect at all doses (100, 10 and 2 mg/kg), which were statistically similar (p > 0.05) to the positive control. This activity was also confirmed at the cellular level with histopathology analysis. Our results suggest the potential application of this carvacrol-rich TVEO in the prevention and management of fungal infections and topical inflammation and deserve further investigation for clinical applications. Furthermore, while the mode of action remains mainly undetermined and should be studied.

scholarly journals Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency

Antibiotics ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 737
Author(s):  
Marina Pekmezovic ◽  
Melina Kalagasidis Krusic ◽  
Ivana Malagurski ◽  
Jelena Milovanovic ◽  
Karolina Stępień ◽  
...  
Keyword(s):  
Fungal Growth ◽  
Pathogenic Fungi ◽  
Trichophyton Mentagrophytes ◽  
Microsporum Gypseum ◽  
Medium Chain Length ◽  
Gold Drugs ◽  
Growth Inhibitory Activity ◽  
Transdermal Application ◽  
Antifungal Efficiency

Novel biodegradable and biocompatible formulations of “old” but “gold” drugs such as nystatin (Nys) and amphotericin B (AmB) were made using a biopolymer as a matrix. Medium chain length polyhydroxyalkanoates (mcl-PHA) were used to formulate both polyenes (Nys and AmB) in the form of films (~50 µm). Thermal properties and stability of the materials were not significantly altered by the incorporation of polyenes in mcl-PHA, but polyene containing materials were more hydrophobic. These formulations were tested in vitro against a panel of pathogenic fungi and for antibiofilm properties. The films containing 0.1 to 2 weight % polyenes showed good activity and sustained polyene release for up to 4 days. A PHA monomer, namely 3-hydroxydecanoic acid (C10-OH), was added to the films to achieve an enhanced synergistic effect with polyenes against fungal growth. Mcl-PHA based polyene formulations showed excellent growth inhibitory activity against both Candida yeasts (C. albicans ATCC 1023, C. albicans SC5314 (ATCC MYA-2876), C. parapsilosis ATCC 22019) and filamentous fungi (Aspergillus fumigatus ATCC 13073; Trichophyton mentagrophytes ATCC 9533, Microsporum gypseum ATCC 24102). All antifungal PHA film preparations prevented the formation of a C. albicans biofilm, while they were not efficient in eradication of mature biofilms, rendering them suitable for the transdermal application or as coatings of implants.

scholarly journals ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF STEM BARK ESSENTIAL OIL CONSTITUENTS OF LITSEA GLUTINOSA C. B. ROB.

2016 ◽  
Vol 8 (12) ◽  
pp. 258
Author(s):  
Arunodaya H. S. ◽  
Krishna V. ◽  
Shashikumar R. ◽  
Girish Kumar K.
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Stem Bark ◽  
Diffusion Method ◽  
Vibrio Cholera ◽  
Litsea Glutinosa ◽  
Β Carotene ◽  
Mic Value

<p><strong>Objective: </strong>To evaluate the chemical composition, antibacterial and antioxidant properties of stem bark essential oil of <em>Litsea glutinosa </em>C. B. Rob.</p><p><strong>Methods: </strong>The essential oil isolated from stem bark of <em>L. glutinosa </em>and their chemical composition was analyzed by gas chromatography coupled with mass spectrometry detector. The <em>in vitro </em>antibacterial activity of the stem bark essential oil was investigated against eight human pathogenic bacterial clinical isolates using agar disc diffusion method and MIC value was determined by modified resazurin microtitre-plate assay. The antioxidant activity of essential oil was measured by 2, 2-diphenyl-1-picrylhydrazyl radical (DPPH), 2, 2-azinobis-3-ethylbenzothiazoline-6-sulphonate radical cation (ABTS) and β-carotene bleaching assay.</p><p><strong>Results: </strong>GC-MS analysis of stem bark essential oil resulted in the identification of 37 compounds, off which 9,12-octadecadienoic acid (62.57%), hexadecanoic acid (12.68%), stigmast-5-en-3-ol (6.87%) and vitamin E (2.51%) were the main constituents representing 84.63% of the oil. The determination of <em>in vitro</em> antibacterial activity of stem bark essential oil resulted in significant inhibition zone (15.00±0.57 mm) and MIC value (0.15±0.15×10<sup>-2</sup> mg/ml) against the pathogenic bacteria <em>Vibrio cholera</em> followed by <em>Pseudomonas aeruginosa</em> and <em>Salmonella typhi. </em>The results of DPPH radical scavenging (IC<sub>50</sub>:4.540±0.06 µg/ml), ABTS (IC<sub>50</sub>:256.02±0.06 µg/ml) and β-carotene bleaching assay (%I: 78.51±0.42 <strong>%</strong>) showed significant <em>in vitro</em> antioxidant property.</p><p><strong>Conclusion: </strong><em>L. glutinosa</em> stem bark essential oil showed potential antibacterial activity against the <em>Vibrio cholera</em>. The results of this investigation supported the ethnomedical claim of essential oil as a demulcent, antidiarrheal and antioxidant drug.</p>

scholarly journals Komposisi Minyak Atsiri dan Aktivitas Antimikroba Rimpang Temu Putih dan Jahe Gajah sebagai Fitobiotik Pakan Unggas

2019 ◽  
Vol 6 (2) ◽  
pp. 181
Author(s):  
Laila Nur Rohma ◽  
Laila Nur Rohma ◽  
Osfar Sjofjan ◽  
M. Halim Natsir
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Feed Additives ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Activity Test ◽  
Main Component

ABSTRAK                                                                        Imbuhan pakan unggas dapat berasal dari bahan herbal yang mengandung berbagai komponen aktif yang bermanfaat bagi pertumbuhan ternak.Temu putih dan jahe gajah dapat dimanfaatkan sebagai imbuhan pakan karena mengandung minyak atsiri yang dapat berperan sebagai agen antibakteri. Penelitian ini bertujuan untuk mengetahui komponen penyusun minyak atsiri dan aktivitas antimikroba pada rimpang temu putih dan jahe gajah. Penelitian dilakukan dengan percobaan in vitro menggunakan temu putih dan jahe gajah yang diolah menjadi bentuk ekstrak minyak atsiri temu putih dan jahe gajah sebagai materi uji komposisi penyusun minyak atsiri serta bentuktepung dan enkapsulasi sebagai materi uji aktivitas antimikroba. Komposisi minyak atsiri temu putih terdiri dari lima komponen penyusun dengan cis-1,7-octadien-3-yl acetat sebagai komponen utama. Komposisi minyak atsiri jahe gajah terdiri dari tujuh komponen dan benzene,1-(1,5-dimethyl-4-hexenyl)-4-methyl-(CAS) ar-curcumene sebagai komponen utama. Minyak atsiri yang terkandung pada temu putih dan jahe gajah mempunyai peran dalam menghambat mikroba. Uji komposisi penyusun minyak atsiri menggunakan alat GC-MS dan uji aktivitas antimikroba menggunakan metode disc diffusion dan. Hasil dari uji aktivitas antimikroba menunjukkan bahwa temu putih dan jahe gajah dalam bentuk tepung dan enkapsulasi memiliki perbedaan yang sangat nyata (P<0,01) terhadap aktivitas antimikroba pada bakteri asam laktat, Escherichia coli dan Salmonella sp. Campuran temu putih dan jahe gajah (1:1) menunjukkan kemampuan terbaik dalam menghambat pertumbuhan bakteri patogen dengan diameter zona hambat 5,70±0,14 mm  (Escherichia coli) dan 6,88±0,45 mm (Salmonella sp.).Kata Kunci : antimikroba, fitobiotik, jahe gajah, minyak atsiri, temu putihABSTRACTThe poultry feed additives can contain herbal ingredients that contain various beneficial components for livestock growth. White turmeric and giant ginger can be used as feed additives because they contain essential oils that can be used as antibacterial agents. This study aims to determine the constituent components of essential oils and antimicrobial activity in white turmeric and giant ginger rhizomes. The study was carried out by in vitro experiments using white turmeric and giant ginger which were processed into the form of essential oil extract as material for the composition of essential oils test, and powder and encapsulation form as antimicrobial activity test material. The composition of essential oils of white turmeric consists of five constituent components with cis-1,7-octadien-3-yl acetate as the main component. The composition of giant ginger essential oil consists of seven components with benzene, 1- (1,5-dimethyl-4-hexenyl) -4-methyl- (CAS) ar-curcumene as the main component. Essential oils contained in the white turmeric and giant ginger have a role in inhibiting microbes. The composition of the essential oil tested using GC-MS and the antimicrobial activity test used the disc diffusion method. The results of the antimicrobial activity test showed that white turmeric and giant ginger in powder and encapsulation form had significant differences (P <0.01) on antimicrobial activity in lactic acid bacteria, Escherichia coli and Salmonella sp. The mixture of white turmeric and giant ginger (1: 1) showed the best ability to inhibit the growth of pathogenic bacteria with inhibitory zone diameters of 5.70 ± 0.14 mm (Escherichia coli) and 6.88 ± 0.45 mm (Salmonella sp.).Keywords: antimicrobial, essential oil, giant ginger, phytobiotic, white turmeric

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK PERASAN DAUN MENGKUDU (Morinda citrifolia L.) TERHADAP Escherichia coli SECARA IN VITRO

2020 ◽  
Vol 2 (1) ◽  
pp. 26-34
Author(s):  
Margareta Retno Priamsari ◽  
Agastia Cicilia Wibowo
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Drying Shrinkage ◽  
Positive Control ◽  
Negative Control ◽  
Morinda Citrifolia ◽  
Diffusion Method ◽  
Juice Extraction ◽  
Water Inhibition

Noni juice can inhibit the growth of Escherichia coli bacteria. Noni juice extraction needs concentration to extract so that the preparation is more stable in the storage process. The purpose of this study was to determine the antibacterial activity and the amount of the minimum inhibitory concentration of noni juice extract from E. coli bacteria in vitro. This type of experimental research with a completely randomized one-way design. The extract was obtained by concentrating the Noni leaf extract. Extract quality control parameters include organoleptic, yield, drying shrinkage, and qualitative tests of flavonoid and anthraquinone compounds. Antibacterial activity test using the disc diffusion method with an extract concentration of 1.56%; 3.12%; 6.25%; 12.5%; and 25% with 3 replications. Positive control of amoxicillin and negative control of distilled water. Inhibition is known from the zone formed around the paper disc. The data obtained were statistically analyzed using Kruskall Wallis followed by Mann Whitney with a 95% confidence level. The results showed that the variation in the concentration of the noni juice extract had a significant effect (p <0.05). The biggest inhibitory zone was seen at 25% concentration of 10.16 mm and included in the strong category. The minimum inhibitory power was produced at a concentration of 3.12% at 2.50 mm with a weak treatment category.

scholarly journals Essential Oil from Ocimum basilicum (Omani Basil): A Desert Crop

2011 ◽  
Vol 6 (10) ◽  
pp. 1934578X1100601 ◽  
Author(s):  
Ahmed Yahya Al-Maskri ◽  
Muhammad Asif Hanif ◽  
Masoud Yahya Al-Maskari ◽  
Alfie Susan Abraham ◽  
Jamal Nasser Al-sabahi ◽  
...  
Keyword(s):  
Essential Oil ◽  
Pathogenic Fungi ◽  
Essential Oil Composition ◽  
Ocimum Basilicum ◽  
Diffusion Method ◽  
Oil Composition ◽  
Different Seasons ◽  
Dpph Scavenging ◽  
Basil Oil

The focus of the present study was on the influence of season on yield, chemical composition, antioxidant and antifungal activities of Omani basil ( Ocimum basilicum) oil. The present study involved only one of the eight Omani basil varieties. The hydro-distilled essential oil yields were computed to be 0.1%, 0.3% and 0.1% in the winter, spring and summer seasons, respectively. The major components identified were L- linalool (26.5 - 56.3%), geraniol (12.1 - 16.5%), 1,8-cineole (2.5 - 15.1%), ρ-allylanisole (0.2 - 13.8%) and DL-limonene (0.2 -10.4%). A noteworthy extra component was β- farnesene, which was exclusively detected in the oil extracted during winter and spring at 6.3% and 5.8%, respectively. The essential oil composition over the different seasons was quite idiosyncratic, in which the principal components of one season were either trivial or totally absent in another. The essential oil extracted in spring exhibited the highest antioxidant activity (except DPPH scavenging ability) in comparison with the oils from other seasons. The basil oil was tested against pathogenic fungi viz. Aspergillus niger, A. fumigatus, Penicillium italicum and Rhizopus stolonifer using a disc diffusion method, and by determination of minimum inhibitory concentration. Surprisingly high antifungal values were found highlighting the potential of Omani basil as a preservative in the food and medical industries.

Sign in / Sign up

Export Citation Format

Share Document