scholarly journals Detection of Babesia spp by using of Acridine Orange and Giemsa Stain with Study of its Effect on Blood Picture in Local Breed Cows.

2019 ◽  
Vol 12 (2) ◽  
Keyword(s):  
Acridine Orange ◽  
Leukocyte Count ◽  
Total Leukocyte Count ◽  
Blood Picture ◽  
Differential Leukocyte Count ◽  
Local Breed ◽  
Acridine Orange Staining ◽  
Fluorescent Microscope ◽  
Blood Smears ◽  
Babesia Spp

This study was conducted to diagnose of Babesia spp by using fluorescent microscope and light microscope, the study also included evaluating of Hemogram (RBC, HB, PCV, MCV, MCHC, Total Leukocyte Count, Differential Leukocyte Count) in cows infected with Babesia spp which cause piroplasmosis. The study included examination of 60 local breed Cows from many districts of Mosul city from the beginning of February 2018 until the end of July 2018, 17 were clinically infected with piroplasmosis and the infection was confirmed by using Giemsa and Acridine orange staining blood smears, and 10 cows were clinically intact and served as control. the results indicated that the percentage of the infection with Babesia spp was 28.33%, and the percentage of parasitemia ranged between (5-23%) with mean of (12.57%). The Results showed a statistical significant decrease (P<0.05) in RBC, Hb, and PCV values of diseased animals, Normocytic Normochromic type of anemia was found, the results also indicated a significant decrease in total Leukocyte count and neutrophil, with significant increase in lymphocytes number. the results also indicated non-significant changes in Eosinophil, Basophil and Monocyte numbers.

scholarly journals Immune function during pregnancy varies between ecologically distinct populations

2020 ◽  
Vol 2020 (1) ◽  
pp. 114-128
Author(s):  
Carmen Hové ◽  
Benjamin C Trumble ◽  
Amy S Anderson ◽  
Jonathan Stieglitz ◽  
Hillard Kaplan ◽  
...  
Keyword(s):  
Leukocyte Count ◽  
Total Leukocyte Count ◽  
Future Research ◽  
Differential Leukocyte Count ◽  
Ecological Conditions ◽  
Trade Offs ◽  
The Usa ◽  
Immunological Changes ◽  
Fetal Antigens ◽  
Pathogen Exposure

Abstract Background and objectives Among placental mammals, females undergo immunological shifts during pregnancy to accommodate the fetus (i.e. fetal tolerance). Fetal tolerance has primarily been characterized within post-industrial populations experiencing evolutionarily novel conditions (e.g. reduced pathogen exposure), which may shape maternal response to fetal antigens. This study investigates how ecological conditions affect maternal immune status during pregnancy by comparing the direction and magnitude of immunological changes associated with each trimester among the Tsimane (a subsistence population subjected to high pathogen load) and women in the USA. Methodology Data from the Tsimane Health and Life History Project (N = 935) and the National Health and Nutrition Examination Survey (N = 1395) were used to estimate population-specific effects of trimester on differential leukocyte count and C-reactive protein (CRP), a marker of systemic inflammation. Results In both populations, pregnancy was associated with increased neutrophil prevalence, reduced lymphocyte and eosinophil count and elevated CRP. Compared to their US counterparts, pregnant Tsimane women exhibited elevated lymphocyte and eosinophil counts, fewer neutrophils and monocytes and lower CRP. Total leukocyte count remained high and unchanged among pregnant Tsimane women while pregnant US women exhibited substantially elevated counts, resulting in overlapping leukocyte prevalence among all third-trimester individuals. Conclusions and implications Our findings indicate that ecological conditions shape non-pregnant immune baselines and the magnitude of immunological shifts during pregnancy via developmental constraints and current trade-offs. Future research should investigate how such flexibility impacts maternal health and disease susceptibility, particularly the degree to which chronic pathogen exposure might dampen inflammatory response to fetal antigens. Lay Summary This study compares immunological changes associated with pregnancy between the Tsimane (an Amazonian subsistence population) and individuals in the USA. Results suggest that while pregnancy enhances non-specific defenses and dampens both antigen-specific immunity and parasite/allergy response, ecological conditions strongly influence immune baselines and the magnitude of shifts during gestation.

scholarly journals The mongolian gerbil: qualitative and quantitative aspects of the cellular blood picture

1975 ◽  
Vol 9 (4) ◽  
pp. 283-287 ◽  
Author(s):  
Walter G. Dillon ◽  
Chester A. Glomski
Keyword(s):  
Haemoglobin Level ◽  
Cell Volume ◽  
Mongolian Gerbil ◽  
Leukocyte Count ◽  
Absolute Number ◽  
Total Leukocyte Count ◽  
Red Cell ◽  
Blood Picture ◽  
Qualitative And Quantitative ◽  
Circulating Lymphocytes

The gerbil displays sex determined differences in packed red-cell volume, haemoglobin level, total leukocyte count and absolute number of circulating lymphocytes. The erythrocytes demonstrate a prominent polychromasia and frequent occurrence of basophilic stippling.

scholarly journals Evaluation of the Effect of Hydroalcoholic Extracts of Cassia Occidentalis Leaves in Neutrophil Adhesion Test in Rats

2020 ◽  
Vol 9 (4-s) ◽  
pp. 1218-1221 ◽  
Author(s):  
Dhaval M Patel ◽  
Advaita B Patel ◽  
Riddhi D Trivedi ◽  
Viram J Parmar ◽  
Urvisha V Bangoriya
Keyword(s):  
Oral Administration ◽  
Leukocyte Count ◽  
Control Group ◽  
Neutrophil Adhesion ◽  
Adhesion Test ◽  
Differential Leukocyte Count ◽  
Hydroalcoholic Extract ◽  
Cassia Occidentalis ◽  
Blood Samples ◽  
Blood Smears

Evaluation of the effect of hydroalcoholic extracts of Cassia occidentalis leaves in neutrophil adhesion test in Rats. The effect of oral administration of hydroalcoholic extract of Cassia occidentalis leaves on neutrophil has been studied and is compared with control group on rats. The differential leukocyte count (DLC) was performed by fixing the blood smears and staining with leucofine and percent neutrophils in each sample was determined. After the initial counts, blood samples were incubated with 80 mg/ml of nylon fibers for 10 min at 37ºC. Supplementation with hydroalcoholic extract of Cassia occidentalis leaves significantly decreased neutrophil adhesion. The results indicate that the leaf of Cassia occidentalis is endowed with protected neutrophil adhesion. These effects could conclude that Cassia occidentalis has an antiasthmatic property. Keywords: Cassia occidentalis, Neutrophil adhesion, analysis of variance

scholarly journals Valores hematológicos do gavião carijó Buteo magnirostris (GMELIN, 1788) (AVE, FALCONIFORME)

2017 ◽  
Vol 2 (1) ◽  
pp. 43-54
Author(s):  
Antenor A. Santos ◽  
Adriana A. Joper ◽  
Mizue I. Egami
Keyword(s):  
Blood Cells ◽  
Leukocyte Count ◽  
Hematological Parameters ◽  
Total Count ◽  
Morphological Description ◽  
Differential Leukocyte Count ◽  
Blood Samples ◽  
Green Area ◽  
Blood Smears ◽  
Spherical Core

RESUMO: Os animais utilizados neste trabalho foram fornecidos pelo Departamento de Parque de Área Verde (SP). As amostras de sangue foram colhidas entre agosto de 1999 a novembro de 2001 e determinados os seguintes parâmetros hematológicos: contagem total de eritrócitos (Er), hematócrito (Ht), taxa de hemoglobina (Hb), contagem total e diferencial de leucócitos, índices hematimétricos absolutos VCM e CHCM. Foi realizada também a descrição morfológica das células sanguíneas em extensões sanguíneas após aplicação do método de Leishman. Os valores referentes à série eritrocítica apresentaram as seguintes médias: Er= 2,62 ± 0.48 x 106/µL; Ht=43,16 ± 11,19%; Hb= 14,59 ± 3,38g/dl; VCM=166,33 fL; CHCM= 33,84 ± 2,55g/dl e àqueles referentes a contagem de leucócitos apresentaram média: Lc= 3,95 ± 2,06 x 103/µL; Ht=45,33 ± 9,48%; Eo=17,66 ± 10,40%; B=7,3 ± 3,05%; L= 27,33 ± 11,53% e M= 2 ± 2,30%. Foram observados eritrócitos elípticos, com núcleo heterocromático alongado e citoplasma acidófilo; trombócitos elípticos com núcleo basófilo, cromatina condensada e citoplasma hialino; heterófilos esféricos, com núcleo lobulado e presença de grânulos acidófilos fusiformes; eosinófilos esféricos com núcleo lobulado apresentando grânulos eosinófilos esféricos; basófilos com núcleo volumoso e grânulos basófilos; linfócitos de aspecto irregular, com núcleo esférico e grânulos azurófilos; monócitos esféricos, com núcleo reniforme, cromatina frouxa, nucléolo visível e grânulos azurófilos finos.Palavras-chave: Gavião Carijó; Buteo magnirostris; Hematologia. ABSTRACT: The animals used in this study were provided by the Green Area Park Department (SP). Blood samples were collected from August 1999 to November 2001 and determined the following hematological parameters: total count of erythrocytes (Er), hematocrit (Ht), hemoglobin (Hb), total count and differential leukocyte count, erythrocyte indices absolute MCV and MCHC. It also performed the morphological description of the blood cells in blood smears after application of Leishman method. The figures for the erythrocyte series presented the following averages: Er = 2.62 ± 0:48 x 106 / µL; Ht = 43.16 ± 11.19%; Hb = 14.59 ± 3,38g / dl; VCM = 166.33 fL; MCHC = 33.84 ± 2.55g / dl and those related to leukocyte count showed average: Lc = 3.95 ± 2.06 x 103 / µL; Ht = 45.33 ± 9.48%; And = 17.66 ± 10.40%; B = 7.3 ± 3.05%; L = 27.33 ± 11.53% and M = 2 ± 2.30%. erythrocytes were observed elliptical, with elongated heterochromatic nucleus and cytoplasm acidophilus; thrombocytes Elliptical with basophil nucleus, condensed chromatin and hyaline cytoplasm; Spherical heterophile with lobed nucleus and presence of acidophilic spindle granules; eosinophil-lobed spherical core having eosinophils spherical granules; basophils with large nuclei and basophil granules; irregular aspect of lymphocytes, with spherical core and azurophilic granules; Spherical monocyte, a kidney-shaped core, loose chromatin and nucleoli visible fine azurophilic granules.Keywords: Sparrowhawk Carijó; Buteo magnirostris; Hematology.

scholarly journals Hematological and Cytometrical Parameters of Japanese Quails Coturnix Coturnix Japonica, Temminck & Schlegel, 1848 as Per Sex and Different Stages of Growth

2021 ◽  
Author(s):  
Ananya Bhattacherjee ◽  
Prafulla Kumar Mohanty ◽  
Bandi Kumar Mallik
Keyword(s):  
Cell Volume ◽  
Japanese Quail ◽  
Packed Cell Volume ◽  
Leukocyte Count ◽  
Age Groups ◽  
Total Leukocyte Count ◽  
Meat Production ◽  
Differential Leukocyte Count ◽  
Coturnix Coturnix ◽  
Length And Area

Abstract This study delineates influence of sex and age on haematological and cytometrical parameters in Japanese quail. Sixty birds were sampled, out of which 20 belong to each age-group (chicks, growers and adults). Haemoglobin, Total Erythrocyte Count, Packed Cell Volume, Erythrocyte indices, Total Leukocyte Count and Differential Leukocyte Count for all 3 age groups and cytometry of blood cells for chicks were analysed. Haemoglobin and packed cell volume of chicks differs significantly with other age-groups at p < 0.01. Significant differences at p = 0.01, p < 0.01 and p < 0.05 observed in lymphocytes, monocytes and Heterophil to Lymphocyte ratio between chicks and growers. Length, length to breadth, nuclear length, breadth, area and Nucleus to Cytoplasm of erythrocytes, length and area of lymphocytes, breadth and area of heterophils also vary significantly at p < 0.01 between Japanese quail chick male and female. Moreover, breadth of erythrocyte and length of heterophil differs significantly at p < 0.05 between two groups. Thus, age and sex influence physiology and hence haematology and may therefore affect egg and meat production.

scholarly journals Bacterial infection in wheezy children.

2021 ◽  
Vol 28 (02) ◽  
pp. 176-180
Author(s):  
Maria Saleem ◽  
Asim Khurshid ◽  
Waqas Imran Khan ◽  
Amna Wajdan
Keyword(s):  
Bacterial Infection ◽  
Absolute Neutrophil Count ◽  
Neutrophil Count ◽  
Leukocyte Count ◽  
Tertiary Care ◽  
Case Series ◽  
Total Leukocyte Count ◽  
X Rays ◽  
Differential Leukocyte Count ◽  
Case Series Study

Objective: To determine the frequency of bacterial infection in children less than five years of age presenting with respiratory wheeze to Tertiary care centre. Study Design: Descriptive Case Series study. Setting: Department of Pediatrics, The Children’s Hospital and The Institute of Child Health, Multan. Period: November 2019 to April 2020. Material & Methods: A total of 130 children, presenting with respiratory symptoms were examined for presence of respiratory wheeze. After confirmation of chest auscultation, patients were enrolled. Information regarding duration of wheeze, axillary temperature, presence of chest in drawing and respiratory rate were recorded. Appropriate blood sample were drawn for total leukocyte count and differential leukocyte count from which absolute neutrophils count was calculated. Children were evaluated for the presence of bacterial infection as per operational definition. Results: There were 38 (29.23%) female and 92 (70.76%) male patients. Overall, mean age was13.17±5.49 with age range of 1-57 months. Bacterial infection was noted in 33 (25.38%) patients. Out of 130 patients presenting with wheeze, 50 patients (38.46%) had temperature range of 98.1-990F. Absolute neutrophil count was more than 5000/dl in 42(32.30%) patients, out of these 33(78.6%) had bacterial infection. Chest X-rays were showing infiltrates in 110 (84.6%) patients. Conclusion: Children suffering from wheeze have bacterial infection if age is less than five years and there is fever, crepitations, elevated absolute neutrophil count and radiographic evidence of pneumonia.

Role of interferon gamma release assay in the diagnosis of Pott disease

2010 ◽  
Vol 12 (5) ◽  
pp. 462-466 ◽  
Author(s):  
Raj Kumar ◽  
Rupant K. Das ◽  
Ashok Kumar Mahapatra
Keyword(s):  
Mr Imaging ◽  
Bone Scan ◽  
Leukocyte Count ◽  
Total Leukocyte Count ◽  
Enzyme Linked Immunosorbent Assay ◽  
Interferon Gamma Release Assay ◽  
Vertebral Collapse ◽  
Diagnostic Dilemma ◽  
Differential Leukocyte Count ◽  
Radiological Criteria

Object Tuberculosis is ubiquitous in its presence and in its myriad manifestations. Despite several advances, it often poses a diagnostic dilemma. The aim of this study was to evaluate the efficacy of the QuantiFERON assay in the diagnosis of Pott disease. Methods In this study, 70 consecutive patients with vertebral collapse underwent a battery of investigations including radiological evaluation (MR imaging and chest and spine radiography), routine blood workup (hemoglobin, total leukocyte count, differential leukocyte count, and erythrocyte sedimentation rate), enzyme-linked immunosorbent assay (ELISA) for tuberculosis, a bone scan and the QuantiFERON assay, perioperative evaluation, and bacteriological studies. The patients were then classified as having tuberculosis on the basis of positive smear or culture, a biopsy consistent with tuberculosis, or a therapeutic response to antituberculosis chemotherapy on follow-up. The efficacy of the investigations, both individually and in combination, were evaluated. Results Tuberculosis was diagnosed in 51 patients, and 19 had vertebral collapse that was attributable to other causes. Based on the authors' data, sensitivity of the QuantiFERON assay in the diagnosis of tuberculosis of the spine was 84% and specificity was 95%. In combination with radiological criteria, bone scan, and ELISA, the QuantiFERON assay was predictive of tuberculosis in 90% of cases (46 patients). Conclusions None of the investigative modalities individually are efficacious enough to make a diagnosis of tuberculosis of the spine with such a degree of certainty as to to obviate the need for a tissue diagnosis or bacteriological confirmation. The ELISA, MR imaging of the spine, and bone scanning findings provide a reasonably certain diagnosis in 90% of cases, providing the grounds for starting a safe trial of antituberculous chemotherapy.

scholarly journals THE EFFECT OF DIET ON THE HEMOGLOBIN, ERYTHROCYTE, AND LEUKOCYTE CONTENT OF THE BLOOD OF THE RHESUS MONKEY (MACACA MULATTA)

Blood ◽  
1947 ◽  
Vol 2 (2) ◽  
pp. 154-163 ◽  
Author(s):  
EDITH SEYMOUR JONES ◽  
KEITH B. MCCALL ◽  
C. A. ELVEHJEM ◽  
P. F CLARK
Keyword(s):  
Ascorbic Acid ◽  
Macaca Mulatta ◽  
Rhesus Monkeys ◽  
Leukocyte Count ◽  
Liver Extract ◽  
Total Leukocyte Count ◽  
Blood Picture ◽  
Neutrophil Lymphocyte Ratio ◽  
B Vitamin ◽  
Nutritional Studies

Abstract 1. Rhesus monkeys fed purified rations supplemented with adequate amounts of the B vitamins, ascorbic acid, and whole liver substance maintained the following average blood picture: See PDF for Table 2. Natural diets or purified rations supplemented with liver extract do not support the above blood picture. The hemoglobin is lower and there is an increase in the range of the total leukocyte count and in the neutrophil-lymphocyte ratio to 2.0 ([unknown]). These figures are similar to the values in the literature and generally accepted as the normal. 3. Previous reports have shown the characteristic blood dyscrasias which develop when monkeys are fed certain B vitamin-deficient diets. These changes are summarized graphically in this paper. 4. The importance of determining the concentration of hemoglobin and the formed elements of the blood as a diagnostic test in nutritional studies has been shown.

scholarly journals Evaluation of the effect of hydroalcoholic extracts of Cassia occidentalis seeds in neutrophil adhesion test in Rats

2021 ◽  
Vol 11 (1-s) ◽  
pp. 117-120
Author(s):  
Dhaval M M Patel ◽  
Advaita B Patel ◽  
Bhumi R Patel ◽  
Riddhi D Trivedi ◽  
Deepa R Patel
Keyword(s):  
Oral Administration ◽  
Leukocyte Count ◽  
Control Group ◽  
Neutrophil Adhesion ◽  
Adhesion Test ◽  
Differential Leukocyte Count ◽  
Hydroalcoholic Extract ◽  
Cassia Occidentalis ◽  
Blood Samples ◽  
Blood Smears

Evaluation of the effect of hydroalcoholic extracts of Cassia occidentalis seeds in neutrophil adhesion test in Rats. The effect of oral administration of hydroalcoholic extract of Cassia occidentalis seeds on neutrophil has been studied and is compared with control group on rats. The differential leukocyte count (DLC) was performed by fixing the blood smears and staining with leucofine and percent neutrophils in each sample was determined. After the initial counts, blood samples were incubated with 80 mg/ml of nylon fibers for 10 min at 37ºC. Supplementation with hydroalcoholic extract of Cassia occidentalis seedss significantly decreased neutrophil adhesion. The results indicate that the seed of Cassia occidentalis is endowed with protected neutrophil adhesion. Keywords: Cassia occidentalis, Neutrophil, analysis of variance

scholarly journals Comparative pathological changes in sheep infected with Theileria annulata and non-infected control

2018 ◽  
Vol 68 (4) ◽  
pp. 629
Author(s):  
W. AKHTER ◽  
A. ASLAM ◽  
M. U. REHMAN ◽  
H. U. REHMAN ◽  
I. RASHID ◽  
...  
Keyword(s):  
Leukocyte Count ◽  
Hemoglobin Concentration ◽  
Theileria Annulata ◽  
Total Leukocyte Count ◽  
Control Group ◽  
Total Proteins ◽  
Blood Smears ◽  
Healthy Control ◽  
Serum Biochemical ◽  
Peripheral Blood Smears

The hematological, serum biochemical and histopathological variations were compared in sheep naturally infected with Theileria annulata and healthy control group. Peripheral blood smears of 300 suspected sheep were observed for the presence of Theileria by microscopy (24%) and confirmed through PCR (34%). The PCR confirmed samples were used for further studies and showed significant decrease in hemoglobin concentration, packed cell volume (PCV), total erythrocyte counts, total leukocyte count, serum total proteins, creatinine and glucose (P < 0.05) as compared to healthy control. Similarly a significant increase was recorded in Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) (P< 0.05) as compared to non-infected sheep. Histopathological changes revealed edema and severe depletion of lymphocytes in lymph nodes. The present study concluded that ovine theileriosis was linked with some pathological alterations in blood and tissues which could be helpful in the diagnosis of disease.

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