scholarly journals Antimicrobial effect of natural kinds of toothpaste on oral pathogenic bacteria

2021 ◽  
Vol 15 (10) ◽  
pp. 1436-1442
Author(s):  
Serdar Demir ◽  
Gul Keskin ◽  
Nese Akal ◽  
Yasemin Zer
Keyword(s):  
Antimicrobial Activity ◽  
Adverse Effects ◽  
Pathogenic Bacteria ◽  
Natural Kinds ◽  
Aloe Vera ◽  
Antimicrobial Effect ◽  
Diffusion Method ◽  
Oral Pathogens ◽  
Inhibition Zones ◽  
Agar Well Diffusion Method

Introduction: Because of the adverse effects on human health of some antimicrobial ingredients in traditional toothpaste, consumers are increasingly turning to toothpastes with natural ingredients. This study evaluates the antimicrobial effect of toothpastes containing different natural active agents against three oral pathogens: Streptococcus mutans, Streptococcus sanguinis, and Enterococcus faecalis. Methodology: This study tested one traditional toothpaste and seven different natural toothpastes containing theobromine, aloe vera, miswak, propolis, chitosan, enzymes and probiotics. The agar-well diffusion method was used to test the antimicrobial effect. Inhibition zones formed around toothpastes after 24 hours of incubation were measured and the data collected were statistically analyzed. Results: Toothpastes containing theobromine and chitosan and the traditional toothpaste showed antimicrobial efficacy for all tested bacteria. Toothpastes containing aloe vera, miswak, and propolis were only effective on S. mutans, while toothpastes containing probiotics and enzymes did not show any antimicrobial effect on the bacteria. Among toothpastes with natural ingredients, the theobromine-containing toothpaste showed the highest efficacy on S. mutans, while the aloe vera- and propolis-containing toothpastes had the lowest efficacy (p < 0.05). Conclusions: Theobromine- and chitosan-containing toothpastes, which showed antimicrobial activity against all bacteria, can be recommended as alternatives to traditional toothpastes.

scholarly journals Synthesis of Silver Nano Particles from Adansonia digitata Leaf Extract and Its Antimicrobial Properties

2020 ◽  
pp. 10-22
Author(s):  
A. O. Akintola ◽  
A. M. Azeez ◽  
B. D. Kehinde ◽  
I. C. Oladipo
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Aspergillus Niger ◽  
Pathogenic Bacteria ◽  
Leaf Extract ◽  
Biomedical Application ◽  
Nano Particles ◽  
Diffusion Method ◽  
Adansonia Digitata ◽  
Inhibition Zones

Silver nano particles (AgNPs) were green synthesized using Adansonia digitata leaf extract. The synthesized silver nano particles were characterized in terms of synthesis, size, shape, morphology and capping functionalities by UV-Visible Spectroscopy, Scanning Electron Microscopy (SEM) and Fourier Transform Infrared Spectroscopy (FTIR). Antimicrobial activity of the synthesized silver nano particles was investigated by well diffusion method. The antibacterial activity of the nano particle was studied against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeurigunosa, Salmonella typhi and Klebsiella pneumonae while the antifungal activity was studied against Candida albicans, Aspergillus niger, Penicillum notatum and Rhizopus stolomifer. The synthesized AgNPs was active against all the studied microorganisms. Staphylococcus aureus was the most susceptible bacterium (inhibition zones ranging from 12.00 to 28.00 mm, MIC: 30 µl, MBC: 50 µl) while Aspergillus niger was the most susceptible fungi (inhibition zones ranging from 10.00 to 18.00 mm, MIC: 90 µl, MFC: 120 µl. In conclusion the synthesized silver nanoparticles was found to have antimicrobial activity against the pathogenic bacteria and fungi tested and hence has a great potential in biomedical application for the treatment of microbial infections.

scholarly journals Antimicrobial Efficacy of Few Commercially Available Herbal and Non Herbal Tooth Pastes Against Clinically Isolated Human Cariogenic Pathogens

2015 ◽  
Vol 5 (1) ◽  
pp. 14-18 ◽  
Author(s):  
Amitava Bora ◽  
Sharmista Sen ◽  
Abhirup Goswami ◽  
Gautam Kumar Kundu ◽  
Biswajit Ghosh
Keyword(s):  
Antimicrobial Activity ◽  
Laboratory Method ◽  
Antimicrobial Efficacy ◽  
Diffusion Method ◽  
Occlusal Caries ◽  
Molar Teeth ◽  
Inhibition Zones ◽  
Research And Education ◽  
Herbal Components ◽  
Agar Well Diffusion Method

Objective: The aim of this study was to compare the antimicrobial efficacy of ten commercially available herbal and non herbal toothpastes. Methods: Ten toothpaste brands (A-J) were collected. A, C, D, E contained Herbal components only, toothpastes B, F, G contained herbal components and fluoride as main anticariogenic agent, toothpastes H and I contained fluoride and triclosan combination and toothpaste J contained fluoride only as main anticariogenic component. Dental caries samples were collected from 6-14 years old children having occlusal caries in permanent fully erupted molar teeth involving enamel and dentin but caries not involving dental pulp and Streptococcus mutans bacteria isolated by laboratory method. The antimicrobial activity was assessed by measuring the inhibition zones by agar well diffusion method. Results: Fluoridated toothpaste with triclosan had maximum antimicrobial activity at all concentrations when compared to Herbal toothpaste. Conclusion: Toothpastes containing fluoride and triclosan are most effective but herbal tooth pastes have the prospect of becoming safer and effective altenative and provide an ideal home care anticariogenic regimen. DOI: http://dx.doi.org/10.3329/bjdre.v5i1.22454 Bangladesh Journal of Dental Research and Education Vol.5(1) 2015: 14-18

scholarly journals Determination of Antimicrobial Activity of Different Essential Oils Obtained from Plants on Staphylococcus aureus Strains Isolated from Foods

2020 ◽  
Vol 8 (4) ◽  
pp. 1012-1017 ◽  
Author(s):  
Hacer Aslan Canberi ◽  
Esra Şentürk ◽  
Simge Aktop ◽  
Pınar Şanlıbaba
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Antimicrobial Effect ◽  
Diffusion Method ◽  
Thyme Oil ◽  
Staphyloccocus Aureus ◽  
Hypericum Perforatum L ◽  
Different Strains

Essential oils (EOs) are known for its antimicrobial activity against several pathogenic bacteria. The present work evaluated the antimicrobial activity of 15 different EOs on survival of different strains of different Staphyloccocus aureus strains isolated from traditional cheeses by disc diffusion method. The most antimicrobial activity on the strains was found as oil thyme oil (mean zone diameter 23.203 mm). Clove oil and black seed oil had the highest antimicrobial activity after thyme oil with average zone diameters of 13.698 mm and 11.267 mm respectively. Hypericum perforatum L. oil (mean zone 6.209 mm), ginger oil (mean zone 6.250 mm) and garlic oil (mean zone 6.267 mm) were the lowest antimicrobial activity. New studies about antimicrobial effect of EOs in vivo conditions are recommended.

scholarly journals The antimicrobial activity of honey, bee pollen loads and beeswax from Slovakia

2012 ◽  
Vol 64 (3) ◽  
pp. 927-934 ◽  
Author(s):  
Miroslava Kacániová ◽  
N. Vukovic ◽  
R. Chlebo ◽  
P. Hascík ◽  
Katarína Rovná ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Pathogenic Bacteria ◽  
Ethanol Extract ◽  
Antimicrobial Effect ◽  
Diffusion Method ◽  
Microscopic Fungi ◽  
Microscopic Fungus ◽  
Bee Pollen ◽  
Pollen Loads ◽  
Different Strains

The aim of this study was to test the antimicrobial activity of propolis, bee pollen loads and beeswax samples collected in the year 2009 from two locations in Slovakia to pathogenic bacteria, microscopic fungi and yeasts. The antimicrobial effect of the bee product samples were tested using the agar well diffusion method. For extraction, 99.9% and 70% methanol (aqueous, v/v) and 96% and 70% ethanol (aqueous, v/v) were used. Five different strains of bacteria, i.e. Listeria monocytogenes ccM 4699, Pseudomonas aeruginosa ccM 1960; Staphylococcus aureus ccM 3953; Salmonella enterica ccM 4420, Escherichia coli ccM 3988, three different strains of microscopic fungi, Aspergillus fumigatus, Aspergillus flavus, Aspergillus niger, and seven different strains of yeasts Candida krusei, Candida albicans, Candida glabrata, Candida parapsilosis, Candida tropicalis, Geotrichum candidum, Rhodotorula mucilaginosa, were tested. After 48 hours S. aureus was the bacterium most sensitive to the 70% ethanol extract of pollen, A. fumigatus was the most sensitive microscopic fungus (70% ethanol) and C. glabrata the most sensitive yeast (70% methanol). Microorganisms most sensitive to propolis extracts were L. monocytogenes, A. fumigatus (70% ethanol) and G. candidum (70% methanol). Most sensitive to beeswax extracts were E. coli, A. niger and C. tropicalis.

COMPOSICIÓN QUÍMICA Y ACTIVIDAD ANTIMICROBIANA DE EXTRACTOS DE MACROALGA Gracilaria vermiculophylla SOBRE Vibrio parahaemolyticus/ CHEMICAL COMPOSITION AND ANTIMICROBIAL ACTIVITY OF DIFFERENT EXTRACTS FROM THE MACROALGAE Gracilaria vermiculophylla AGAINST

Biotecnia ◽  
1969 ◽  
Vol 18 (2) ◽  
pp. 27-31
Author(s):  
Pablo Sergio Osuna Amarillas ◽  
Anselmo Miranda Baeza ◽  
Martha Elisa Rivas Vega ◽  
Edgard Esquer Miranda ◽  
Daniel García Bedoya ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Vibrio Parahaemolyticus ◽  
Pathogenic Bacteria ◽  
Antimicrobial Effect ◽  
Marine Macroalgae ◽  
Diffusion Method ◽  
Gracilaria Vermiculophylla ◽  
Agar Diffusion Method

La búsqueda de compuestos naturales como una alternativa ante el uso de antibióticos sintéticos ha tomado mucha importancia. Se ha demostrado que las macroalgas marinas contienen compuestos capaces de inhibir el crecimiento bacteriano, incluyendo bacterias patógenas para el humano. El objetivo del presente trabajo fue determinar el efecto de dos extractos (acetona y metanol) de la macroalga Gracilaria vermiculophylla sobre el crecimiento de la bacteria Vibrio parahaemolyticus. La determinación del efecto antimicrobiano de los extractos crudos se llevó a cabo utilizando el método de difusión en disco; para lo cual se utilizaron 10, 30 y 50 μL de los extractos en los sensidiscos. Se determinó que el volumen de 50 μL, con ambos extractos presentó los halos de inhibición más altos (3.24 mm para el extracto en metanol y 3.17 mm para el extracto en acetona). ABSTRACT The search for natural compounds as an alternative to the use of synthetic antibiotics has become increasingly important. It has been shown that the marine macroalgae contain compounds capable of inhibiting bacterial growth, including pathogenic bacteria for humans. The aim of this study was to determine the effect of two extracts (acetone and methanol) of macroalgae Gracilaria vermiculophylla on the growth of Vibrio parahaemolyticus. Determination of antimicrobial effect of the crude extracts was performed using the agar diffusion method; for which 10, 30 and 50 μL of the extracts were loaded on sterile antibiotic discs. It was determined that the 50 μL with both extracts showed higher inhibiting zone diameter (3.24 mm for the extract in methanol and 3.17 mm for the extract in acetone).

scholarly journals Comparative antimicrobial potential of Tribhuvana-Mishrana and its ingredients against clinical bacteria

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Vashisht Kiran ◽  
Thakur Vivek ◽  
Kumar Deepak ◽  
Sharma Khemchand
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Effect ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Single Drug ◽  
Microbial Infections ◽  
Agar Well Diffusion Method

There are many single drug and compound formulations prescribed for Jwara (~ fever) in Ayurvedic classics. Tribhuvanakirti Rasa (TKR), Godanti Bhasma (GB) and Sudarshana Ghana Vati (SGV) are widely used compound formulations that are separately indicated for the treatment of Jwara (~fever) in different dosage schedules. In Ayurvedic Formulary of India Tribhuvana-Mishrana (TM) has been chiefly indicated in treatment of Sarva Jwara (~ fever). Therefore, in this study comparative antimicrobial activity of TM and its individual ingredients viz. TKR, GB and SGV were evaluated against four fever-causing microbes such as gram-positive bacteria Staphylococcus aureus and the gram-negative bacteria Salmonella typhii, Escherichia coli and Pseudomonas aeruginosa. The aqueous extract of all the four samples (formulation as whole and the three individual ingredients separately) in a dose 125mcg/ml were tested for their antimicrobial activity against four different microbes by agar well diffusion method. The study shows that TM and its ingredients independently show significant antimicrobial activity. S. aureus was found to be most sensitive to SGV and moderately sensitive to TKR and TM while S. typhii was found to be most sensitive to TM as compared to others. TKR was found to be most effective against E.coli while SGV and TM both showed significant antimicrobial effect against E. coli. TM could provide an alternative to synthetic antibiotics against human microbial infections.

scholarly journals An In Vitro Study on the Antimicrobial Properties of Essential Oil Modified Resin Composite against Oral Pathogens

Materials ◽  
2020 ◽  
Vol 13 (19) ◽  
pp. 4383
Author(s):  
Barbara Lapinska ◽  
Aleksandra Szram ◽  
Beata Zarzycka ◽  
Janina Grzegorczyk ◽  
Louis Hardan ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Antimicrobial Agents ◽  
Resin Composite ◽  
Antimicrobial Properties ◽  
In Vitro Study ◽  
Diffusion Method ◽  
Oral Pathogens

Modifying the composition of dental restorative materials with antimicrobial agents might induce their antibacterial potential against cariogenic bacteria, e.g., S.mutans and L.acidophilus, as well as antifungal effect on C.albicans that are major oral pathogens. Essential oils (EOs) are widely known for antimicrobial activity and are successfully used in dental industry. The study aimed at evaluating antibacterial and antifungal activity of EOs and composite resin material (CR) modified with EO against oral pathogens. Ten EOs (i.e., anise, cinnamon, citronella, clove, geranium, lavender, limette, mint, rosemary thyme) were tested using agar diffusion method. Cinnamon and thyme EOs showed significantly highest antibacterial activity against S.mutans and L.acidophilus among all tested EOs. Anise and limette EOs showed no antibacterial activity against S.mutans. All tested EOs exhibited antifungal activity against C.albicans, whereas cinnamon EO showed significantly highest and limette EO significantly lowest activity. Next, 1, 2 or 5 µL of cinnamon EO was introduced into 2 g of CR and microbiologically tested. The modified CR showed higher antimicrobial activity in comparison to unmodified one. CR containing 2 µL of EO showed the best antimicrobial properties against S.mutans and C.albicans, while CR modified with 1 µL of EO showed the best antimicrobial properties against L.acidophilus.

Biosynthesis of Silver Nanoparticles by Punica Granatum Peel Extract and their Biological Activity on different Pathogenic Bacteria

2021 ◽  
Vol 19 (9) ◽  
pp. 38-45
Author(s):  
Hussein H. Al-Turnachy ◽  
Fadhilk. alibraheemi ◽  
Ahmed Abd Alreda Madhloom ◽  
Zahraa Yosif Motaweq ◽  
Nibras Yahya Abdulla
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Biological Activity ◽  
Pathogenic Bacteria ◽  
Punica Granatum ◽  
Gram Positive ◽  
Gram Negative ◽  
Inhibition Zones ◽  
Peel Extract

The present study was included the assessment of the antimicrobial activity of AgNPs synthesized by Punica granatum peel extract against pathogenic bacteria by testing warm aqueous P. granatum peel extract and silver nanoparticles. Punica granatum indicated potency for AgNP extracellular nanobiosynthesis after addition of silver nitrate (AgNO3) 4mM to the extract supernatant, in both concentrations (100mg and 50mg). The biogenic AgNPs showed potency to inhibit both gram-negative and gram-positive bacterial growth. Zons of inhibition in (mm) was lesser in gram-positive than gram-negative bacteria. The resulted phytogenic AgNPs gave higher biological activity than warm aqueous Punica granatum peel extract. The inhibition zone of the phytogenic AgNPs on E. coli reached 17.53, 22.35, and 26.06 mm at (0.1, 0.5, and 1) mg/ml respectively. While inhibition zones of Punica warm aqueous extract reached 5.33, 10.63, and 16.08 mm at the same concentrations. phytogenic AgNPs gave smaller inhibition zones in gram-positive than gram- negative. Cytotoxic activity of the phytogenic AgNPs was assayed in vitro agaist human blood erythrocytes (RBCs), spectroscopic results showed absorbance at 540 nm hemolysis was observed. In general, AgNPs showed least RBCs hemolysis percentage, at 1 mg/ml concentration, hemolysis percentage was (4.50%). This study, concluded that the Punica granatum peel extract has the power of synthses of AgNPs characterized by broad spectrum antimicrobial activity with cyto-toxicity proportional to AgNPs concentration.

scholarly journals Evaluation of the antimicrobial effect of calcium hydroxide combined to different vehicles

2016 ◽  
Vol 31 (2) ◽  
pp. 89
Author(s):  
Tatiana Dantas Piana ◽  
Maria de Fátima Malvar Gesteira ◽  
Erica Dos Santos Carvalho ◽  
Josilene Borges Torres Lima Matos ◽  
Monica Franca ◽  
...  
Keyword(s):  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Antimicrobial Effect ◽  
Antimicrobial Action ◽  
Diffusion Method ◽  
Agar Diffusion ◽  
Inhibition Zones ◽  
Agar Diffusion Method

Objective: To evaluate the potentiating effect of different substances in antimicrobial action of calcium hydroxide.Methods: The agar diffusion method, was used with well technique, to analyze seven substances associated to calcium hydroxide to make some pastes, they are: chlorexidine, saline, anesthetic, malvatricin, propolis, hypochlorite, paramonochlorophenol.Results: The pastes with malvatricin and paramonochlorophenol presented greater inhibition zones against Enterococcus faecalis.Conclusion: There was potentialization in antimicrobial effect of calcium hydroxide when associated to paramonochlorophenol and to malvatricin especially against Enterococcus faecalis. Low increase of the antimicrobial capacity was observed when using chlorexidine as vehicle to calcium hydroxine paste.

scholarly journals Antibacterial activity of ginger extracts and its essential oil on some of pathogenic bacteria

2010 ◽  
Vol 7 (3) ◽  
pp. 1159-1165
Author(s):  
Baghdad Science Journal
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Pathogenic Bacteria ◽  
Cold Water ◽  
Water Extract ◽  
Ethanolic Extract ◽  
Hot Water ◽  
Diffusion Method ◽  
Resistant Bacteria

The antimicrobial activity of ginger extracts ( cold-water, hot-water, ethanolic and essential oil ) against some of pathogenic bacteria ( Escherichia coli , Salmonella sp , Klebsiella sp , Serratia marcescens, Vibrio cholerae , Staphylococcus aureus , Streptococcus sp) was investigated using Disc diffusion method , and the results were compared with the antimicrobial activity of 12 antibiotics on the same bacteria . The results showed that the ginger extracts were more effective on gram-positive bacteria than gram-negative . V. cholerae and S. marcescens,were the most resistant bacteria to the extracts used , while highest inhibition was noticed against Streptococcus sp (28 mm) . The ethanolic extract showed the broadest antibacterial activity ( 11 to 28 mm ) , in comparison with moderate activity of essential oil , it was observed that the cold-water extract was more effective on the bacteria than hot-water extract . Ginger ethanolic extract presented higher diameter of inhibition zone for Streptococcus sp than in Ciprofloxacin , Cefotaxime , Cefalotin , Cephalexin and Cephaloridine , also it was found a similarity between the higher inhibition zones of ethanolic extract of ginger and some antibiotics for S. aureus , E. coli , Salmonella sp and Klebsiella sp . V. cholerae and S. marcescens,also highly resistant to antibiotics . Phytochemical analysis of ethanolic extract of ginger revealed the present of glycosides, terpenoids, flavonids and phenolic compounds

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