scholarly journals Antibiotic resistance patterns of bacterial strains isolated from Periplaneta americana and Musca domestica in Tangier, Morocco

2010 ◽  
Vol 4 (04) ◽  
pp. 194-201 ◽  
Author(s):  
Lamiaa Bouamamaa ◽  
Antonio Sorlozano ◽  
Amin Laglaoui ◽  
Mariam Lebbadi ◽  
Ahmed Aarab ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Musca Domestica ◽  
Pathogenic Bacteria ◽  
Periplaneta Americana ◽  
Human Beings ◽  
Residential Areas ◽  
Bacterial Strains ◽  
Human Pathogenic Bacteria ◽  
Enterococcus Spp ◽  
Staphylococcus Spp

Background:  Flies and cockroaches are two insects in close contact with human beings. They are carriers of human pathogenic bacteria on the external areas of their bodies or in their digestive tracts. This study examines Periplaneta americana and Musca domestica collected from the residential areas of six districts in Tangier, Morocco. Methodology: In total, 251 bacteria were isolated from external areas of the participants' bodies and the antimicrobial susceptibility was calculated. Results: The predominant bacterial species included Escherichia coli (17.9%), Klebsiella spp. (14.7%), Providencia spp. (9.6%), Staphylococcus spp. (15.1%) and Enterococcus spp. (11.6%). The study showed no difference between the species of bacterial strains from American cockroaches and houseflies. Carbapenems and aminoglycosides were active against 100% of the Gram-negative bacilli isolated in this study. Staphylococcus spp. strains were susceptible to linezolid, vancomycin, daptomycin, levofloxacin and cotrimoxazole, and no antibiotic resistance was found in Enterococcus spp. Conclusions: In our setting, although both cockroaches and flies collected from residential areas may be vectors of human pathogenic bacteria, the infections caused by them are easily treatable as a result of the high susceptibility of their bacteria to antibiotics routinely used in the community or in hospitals.

scholarly journals Antibacterial Compounds from Mushrooms: A Lead to Fight ESKAPEE Pathogenic Bacteria?

Planta Medica ◽  
2020 ◽  
Author(s):  
Violette Hamers ◽  
Clément Huguet ◽  
Mélanie Bourjot ◽  
Aurélie Urbain
Keyword(s):  
Antibiotic Resistance ◽  
Global Health ◽  
Pathogenic Bacteria ◽  
Resistance Mechanisms ◽  
Pathogenic Microorganisms ◽  
Bacterial Strains ◽  
Antibacterial Compounds ◽  
New Antibiotics ◽  
Hospital Stays ◽  
Antibacterial Potential

AbstractInfectious diseases are among the greatest threats to global health in the 21st century, and one critical concern is due to antibiotic resistance developed by an increasing number of bacterial strains. New resistance mechanisms are emerging with many infections becoming more and more difficult if not impossible to treat. This growing phenomenon not only is associated with increased mortality but also with longer hospital stays and higher medical costs. For these reasons, there is an urgent need to find new antibiotics targeting pathogenic microorganisms such as ESKAPEE bacteria. Most of currently approved antibiotics are derived from microorganisms, but higher fungi could constitute an alternative and remarkable reservoir of anti-infectious compounds. For instance, pleuromutilins constitute the first class of antibiotics derived from mushrooms. However, macromycetes still represent a largely unexplored source. Publications reporting the antibacterial potential of mushroom extracts are emerging, but few purified compounds have been evaluated for their bioactivity on pathogenic bacterial strains. Therefore, the aim of this review is to compile up-to-date data about natural products isolated from fruiting body fungi, which significantly inhibit the growth of ESKAPEE pathogenic bacteria. When available, data regarding modes of action and cytotoxicity, mandatory when considering a possible drug development, have been discussed in order to highlight the most promising compounds.

scholarly journals Evaluation of sanitary quality of goat milk in dairy industries from the Cariri region, state of Paraíba

2021 ◽  
Vol 10 (12) ◽  
pp. e596101220735
Author(s):  
Iara Nunes de Siqueira ◽  
Aline Antas Cordeiro Cavalcanti ◽  
Joyce Galvão de Souza ◽  
Filipe Jordão Pereira de Medeiros ◽  
João Carlos Taveira ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Pathogenic Bacteria ◽  
Goat Milk ◽  
Salmonella Spp ◽  
Pasteurized Milk ◽  
Enterococcus Spp ◽  
Bacillus Spp ◽  
Thermotolerant Coliforms ◽  
Staphylococcus Spp

The sanitary evaluation of equipment and hands is fundamental to investigate the presence of pathogens in the dairy industry. Then, this study aims to evaluate the sanitization of equipment, workers’ hands, raw and pasteurized milk in goat milk dairies in the Cariri region, state of Paraíba.  Collected 32 samples of four dairies represented by letters A, B, C, and D. The followings contents were analyzed: mesophiles, total and thermotolerant coliforms, Escherichia coli, Staphylococcus aureus, Samonella spp. and Listeria monocytogenes in the reception tank, pasteurization tank, packing machine, package, wall, workers’ hand, and each dairy’s raw and pasteurized milk. After isolation, 84 colonies were confirmed by MALDI TOF. The indicator microorganisms presented variations for the workers’ hands, while A and B stayed within the patterns. For the equipment, only dairy B was within limits. They were out of the standard for mesophiles, total coliforms, and thermotolerant regarding raw and pasteurized milk. The microorganisms, the Enterobacteriaceae family presented a higher frequency, with 77.38%, and within this family, Escherichia coli, Klebsiella spp., and Enterobacter spp. were the most prevalent. Gram-positive corresponded to 22.62%, Bacillus spp., Staphylococcus spp., Enterococcus spp., and Macrococcus caseolyticus. Listeria monocytogenes and Salmonella spp. were not isolated. These demonstrate failures in goat milk processing with pathogenic bacteria in several dairy plants, indicating the need to adjust the product’s quality control.

scholarly journals Antibiotic Sensitivity and Resistance Among the Most Common Uropathogens in Kidney Transplant Recipients

2015 ◽  
Vol 12 (1) ◽  
pp. 27-32
Author(s):  
Borelli Zlatkov ◽  
Jean Filipov ◽  
Emil Paskalev ◽  
Boyka Markova ◽  
Yuliya Marteva-Proevska ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Kidney Transplant ◽  
Antibiotic Sensitivity ◽  
High Sensitivity ◽  
Transplant Recipients ◽  
Kidney Transplant Recipients ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Transplant Center ◽  
Enterococcus Spp

Abstract Introduction. Urinary tract infection (UTI) among kidney transplant recipients (KTRs) is one of the most common complications after transplantation. The aim of our study was to analyze the antibiotic sensitivity and resistance of the most common agents causing UTI in Bulgarian KTRs followed up in our Transplant Center. Methods. We analyzed the antibiotic resistance and sensitivity of the most common strains of bacteria causing UTI in the Bulgarian KTRs, namely class Enterobac-teriaceae and Enterococcus spp. We used conventional biochemical methods to identify different strains of uro-pathogens-miniApi (bioMerieux, France) and BBL Crystal (BD). The antibiotic sensitivity was determined via disc-diffusing method, according to the accepted Bulgarian CLSI standard. We used WHONET, version 5.6 to analyze the antibiotic resistance data. Results. The total number of tested patients was 366 [males 228, females 138]. The total number of tested urine samples was 829 [positive ones-203), negative samples 606, contaminated 20]. The most commonly detected uropathogens in Bulgarian KTRs were Gram /-/ negative bacteria (63.80%). Of these, 93.28% belonged to the Enterobacteriaceae group, with E. coli, K. pneumoniae and the PPM /Proteus, Providentia, Mor-ganela/subgroup being the most common (54.5%, 19.20% and 16%, respectively). Gram /+/ positive bacteria were detected in 28.09% of the patients, Enterococcus spp being the most commonly isola-ted-67.79%. In the Enterococcus group, the strains of E. faecalis and E. faecium were the most commonly detected. The bacteria belonging to Enterobacteriaceae group were most sensitive to carbapenems and aminoglycosides, with sensitivity peaking to almost 100%, whereas they were least sensitive to aminopenicillines [sensitivity below 20%]. The PPM subgroup revealed very high sensitivity to beta-lactamase protected broad spectrum penicillins (Piperacillin/Tazobactam, sensitivity - 90%). Gram /+/ positive uropathogens were mostly sensitive to Linezolid, Vancomycin, Teicoplanin (100%). These strains were least sensitive to Erythromycin and Tetracicline (17.50%). Conclusions. Our results were similar to previous studies. The differences detected can be explained with the characteristics of the bacterial strains and the specific practice of each transplant center. Having in mind the possible complications of UTIs, further studies are needed to clarify the problem with antimicrobial resistance in uropathogens and the use of antibiotics after KT.

Antimicrobial Effect of Brazillian Propolis/Polycaprolactone Polymer on some Human Pathogenic Bacteria

2012 ◽  
Vol 506 ◽  
pp. 537-540 ◽  
Author(s):  
S. Sanpa ◽  
Krit Sutjarittangtham ◽  
Tawee Tunkasiri ◽  
Sukum Eitssayeam ◽  
P. Chantawannakul
Keyword(s):  
Pathogenic Bacteria ◽  
Antimicrobial Effect ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Electrospinning Technique ◽  
Propolis Extract ◽  
Human Pathogenic Bacteria ◽  
Polymer Nanofiber ◽  
History Of Use

The antimicrobial activities of propolis against some bacterial strains have been studied. However, there are few reports on biopolymers from propolis. Polycaprolactone (PCL) is a biodegradable polymer that has a long history of use in the biomedical field. In this work, the polymer nanofiber was grown from a Brazillian propolis extract solution on PCL using an electrospinning technique. Antimicrobial activities were investigated by the disc diffusion method. The propolis extract and polymer nanofiber could inhibit some human pathogenic bacteria. In addition, the inhibitory effects on tested microorganism depended on the concentration of propolis extract mixed with the polymer nanofiber. Such properties of the propolis/PCL nanofiners can be further developed for biomedical applications.

scholarly journals A Preliminary Study on Antimicrobial Susceptibility of Staphylococcus spp. and Enterococcus spp. Grown on Mannitol Salt Agar in European Wild Boar (Sus scrofa) Hunted in Campania Region—Italy

Animals ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 85
Author(s):  
Francesca Paola Nocera ◽  
Gianmarco Ferrara ◽  
Emanuela Scandura ◽  
Monica Ambrosio ◽  
Filomena Fiorito ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Wild Boar ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Mannitol Salt Agar ◽  
Campania Region ◽  
Wild Boars ◽  
Nasal Cavities ◽  
Enterococcus Spp ◽  
Staphylococcus Spp

The importance of wild boar lies in its role as a bioindicator for the control of numerous zoonotic and non-zoonotic diseases, including antibiotic resistance. Mannitol Salt Agar (MSA) is a selective medium used for isolation, enumeration, and differentiation of pathogenic staphylococci. Other genera such as Enterococcus spp. are also salt tolerant and able to grow on MSA. The present study focused on the identification, by matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS), of bacteria grown on MSA isolated from the nasal cavities of 50 healthy wild boars hunted in Campania Region (southern Italy) in the year 2019. In addition, the antimicrobial resistance phenotype of the isolated strains was determined by disk diffusion method. Among genus Staphylococcus, coagulase-negative Staphylococcus (CoNS) were the most common isolated species, with Staphylococcus xylosus as the most prevalent species (33.3%). Furthermore, Enterococcus spp. strains were isolated, and Enterococcus faecalis was the species showing the highest frequency of isolation (93.8%). For staphylococci, high levels of resistance to oxacillin (93.3%) were recorded. Differently, they exhibited low frequencies of resistance to tested non-β-lactams antibiotics. Among enterococci, the highest resistances were observed for penicillin (93.7%), followed by ampicillin (75%), and ciprofloxacin (68.7%). Interestingly, 43.7% of the isolated strains were vancomycin-resistant. In conclusion, this study reports the phenotypic antibiotic resistance profiles of Staphylococcus spp. and Enterococcus spp. strains isolated from nasal cavities of wild boars hunted in Campania Region, highlighting that these wild animals are carriers of antibiotic resistant bacteria.

scholarly journals Conjugation dynamics of self-transmissible and mobilisable plasmids intoE. coliO157:H7 onArabidopsis thalianarosettes

2018 ◽  
Author(s):  
Mitja N.P. Remus-Emsermann ◽  
Cosima Pelludat ◽  
Pascal Gisler ◽  
David Drissner
Keyword(s):  
Antibiotic Resistance ◽  
Pathogenic Bacteria ◽  
Antibiotic Resistance Genes ◽  
Laboratory Strain ◽  
Time Frame ◽  
Plasmid Transfer ◽  
Intermediate Hosts ◽  
E Coli ◽  
Plant Parts ◽  
Human Pathogenic Bacteria

AbstractMany antibiotic resistance genes present in human pathogenic bacteria are believed to originate from environmental bacteria and conjugation of antibiotic resistance conferring plasmids is considered to be one of the major reasons for the increasing prevalence of antibiotic resistances. A hotspot for plasmid-based horizontal gene transfer is the phyllosphere,i.e.the surfaces of aboveground plant parts. Bacteria in the phyllosphere might serve as intermediate hosts with transfer capability to human pathogenic bacteria. In this study, the exchange of mobilisable and self-transmissible plasmids via conjugation was evaluated. The conjugation from the laboratory strainE. coliS17-1, the model phyllosphere colonizerPantoea eucalypti299R, and the model pathogenE. coliO157:H7∆stxto the recipient strainE. coliO157:H7∷MRE1O3∆stxin the phyllosphere ofArabidopsis thalianawas determined. The results suggest that short-term occurrence of a competent donor is sufficient to fix plasmids in a recipient population ofE. coliO157:H7∷MRE1O3∆stx. The spread of self-transmissible plasmids was limited after initial steep increases of transconjugants that contributed up to 10% of the total recipient population. The here-presented data of plasmid transfer will be important for future modelling approaches to estimate environmental spread of antibiotic resistance in agricultural production environments.ImportanceThis study investigated the transfer of antibiotic resistance conferring plasmids to enteropathogenicE. colion plant leaf surfaces. The results indicate that plasmid transfer may be high within the first 24 hours after inoculation. Transconjugant populations are maintained and stable for a considerable time frame on plant leaves, but invasion of the plasmid to the recipient population is limited.

scholarly journals Identification of antibiotic resistance proteins via MiCId's augmented workflow. A mass spectrometry-based proteomics approach

2021 ◽  
Author(s):  
Gelio Alves ◽  
Aleksey Y Ogurtsov ◽  
Roger Karlsson ◽  
Daniel Jaen-Luchoro ◽  
Beatriz Pineiro-Iglesias ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Antibiotic Resistance ◽  
Pathogenic Bacteria ◽  
High Sensitivity ◽  
Multidrug Resistant ◽  
Bacterial Strains ◽  
Resistance Proteins ◽  
Proteomics Approach ◽  
Resistance Protein ◽  
Microorganism Classification

Fast and accurate identifications of pathogenic bacteria along with their associated antibiotic resistance proteins are of paramount importance for patient treatments and public health. While mass spectrometry has become an important, technique for diagnostics of infectious disease, there is a need for mass spectrometry workflows offering this capability. To meet this need, we have augmented the previously published Microorganism Classification and Identification (MiCId) workflow for this capability. To evaluate the performance of the newly augmented MiCId workflow, we have used MS/MS datafiles from samples of 10 antibiotic resistance bacterial strains belonging to three different species: Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa. The evaluation results show that MiCId's workflow has a sensitivity value around 85% (with a lower bound at about 72%) and a precision greater than 95% in the identification of antibiotic resistance proteins. Using MS/MS datasets from samples of two bacterial clonal isolates, one being antibiotic-sensitive while the other (obtained from the same patient at different times) being multidrug-resistant, we applied MiCId's workflow to investigate possible mechanisms of antibiotic resistance in these pathogenic bacteria; the results showed that MiCId's conclusions are in agreement with the published study. Furthermore, we show that MiCId's workflow is fast. It provides microorganismal identifications, protein identifications, sample biomass estimates, and antibiotic resistance protein identifications in 6-17 minutes per MS/MS sample using computing resources that are available in most desktop and laptop computers, making it a highly portable workflow. This study demonstrated that MiCId's workflow is fast, portable, and with high sensitivity and high precision, making it a valuable tool for rapid identifications of bacteria as well as detection of their antibiotic resistance proteins. The new version of MiCId (v.07.01.2021) is freely available for download at https://www.ncbi.nlm.nih.gov/CBBresearch/Yu/downloads.html.

Sign in / Sign up

Export Citation Format

Share Document